Evolved cas9 proteins for gene editing

ABSTRACT

Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for enginerring Cas9 and Cas9 variants that have increased activity on target sequences that do not contain the canonical PAM sequence. In some embodiments, fusion proteins comprising such Cas9 variants and nucleic acid editing domains, e.g., deaminase domains, are provided.

RELATED APPLICATIONS

This application claims priority under 35 U.S.C. § 119(e) to U.S. provisional patent applications, U.S. Ser. No. 62/245,828 filed Oct. 23, 2015, U.S. Ser. No. 62/279,346 filed Jan. 15, 2016, U.S. Ser. No. 62/311,763 filed Mar. 22, 2016, U.S. Ser. No. 62/322,178 filed Apr. 13, 2016, U.S. Ser. No. 62/357,352 filed Jun. 30, 2016, U.S. Ser. No. 62/370,700 filed Aug. 3, 2016, U.S. Ser. No. 62/398,490 filed Sep. 22, 2016, U.S. Ser. No. 62/408,686 filed Oct. 14, 2016, and U.S. Ser. No. 62/357,332 filed Jun. 30, 2016; each of which is incorporated herein by reference

BACKGROUND OF THE INVENTION

Targeted editing of nucleic acid sequences, for example, the targeted cleavage or the targeted introduction of a specific modification into genomic DNA, is a highly promising approach for the study of gene function and also has the potential to provide new therapies for human genetic diseases.¹ An ideal nucleic acid editing technology possesses three characteristics: (1) high efficiency of installing the desired modification; (2) minimal off-target activity; and (3) the ability to be programmed to edit precisely any site in a given nucleic acid, e.g., any site within the human genome.² Current genome engineering tools, including engineered zinc finger nucleases (ZFNs),³ transcription activator like effector nucleases (TALENs),⁴ and most recently, the RNA-guided DNA endonuclease Cas9,⁵ effect sequence-specific DNA cleavage in a genome. This programmable cleavage can result in mutation of the DNA at the cleavage site via non-homologous end joining (NHEJ) or replacement of the DNA surrounding the cleavage site via homology-directed repair (HDR).^(6,7)

One drawback of the current technologies is that both NHEJ and HDR are stochastic processes that typically result in modest gene editing efficiencies as well as unwanted gene alterations that can compete with the desired alteration.⁸ Since many genetic diseases in principle can be treated by effecting a specific nucleotide change at a specific location in the genome (for example, a C to T change in a specific codon of a gene associated with a disease),⁹ the development of a programmable way to achieve such precise gene editing would represent both a powerful new research tool, as well as a potential new approach to gene editing-based human therapeutics.

Another drawback of current genome engineering tools is that they are limited with respect to the DNA sequences that can be targeted. When using ZNFs or TALENS, a new protein must be generated for each individual target sequence. While Cas9 can be targeted to virtually any target sequence by providing a suitable guide RNA, Cas9 technology is still limited with respect to the sequences that can be targeted by a strict requirement for a protospacer-adjacent motif (PAM), typically of the nucleotide sequence 5′-NGG-3′, that must be present immediately adjacent to the 3′-end of the targeted DNA sequence in order for the Cas9 protein to bind and act upon the target sequence. The PAM requirement thus limits the sequences that can be efficiently targeted by Cas9 proteins.

SUMMARY OF THE INVENTION

Significantly, 80-90% of protein mutations responsible for human disease arise from the substitution, deletion, or insertion of only a single nucleotide.⁶ Most current strategies for single-base gene correction include engineered nucleases (which rely on the creation of double-strand breaks, DSBs, followed by stochastic, inefficient homology-directed repair, HDR), and DNA-RNA chimeric oligonucleotides.²² The latter strategy involves the design of a RNA/DNA sequence to base pair with a specific sequence in genomic DNA except at the nucleotide to be edited. The resulting mismatch is recognized by the cell's endogenous repair system and fixed, leading to a change in the sequence of either the chimera or the genome. Both of these strategies suffer from low gene editing efficiencies and unwanted gene alterations, as they are subject to both the stochasticity of HDR and the competition between HDR and non-homologous end-joining, NHEJ.²³⁻²⁵ HDR efficiencies vary according to the location of the target gene within the genome,²⁶ the state of the cell cycle,²⁷ and the type of cell/tissue.²⁸ The development of a direct, programmable way to install a specific type of base modification at a precise location in genomic DNA with enzyme-like efficiency and no stochasticity therefore represents a powerful new approach to gene editing-based research tools and human therapeutics.

The clustered regularly interspaced short palindromic repeat (CRISPR) system is a recently discovered prokaryotic adaptive immune system¹⁰ that has been modified to enable robust and general genome engineering in a variety of organisms and cell lines.¹¹ CRISPR-Cas (CRISPR-associated) systems are protein-RNA complexes that use an RNA molecule (sgRNA) as a guide to localize the complex to a target DNA sequence via base-pairing.¹² In the natural systems, a Cas protein then acts as an endonuclease to cleave the targeted DNA sequence.¹³ The target DNA sequence must be both complementary to the sgRNA and also contain a “protospacer-adjacent motif” (PAM) at the 3′-end of the complementary region in order for the system to function.¹⁴ The requirement for a PAM sequence limits the use of Cas9 technology, since not all desired targeted sequences include a PAM sequence at the 3′-end and thus cannot efficiently be targeted by wild-type Cas9 proteins.

Provided herein are novel Cas9 variants that exhibit activity on target sequences that do not include the canonical PAM sequence (5′-NGG-3′, where N is any nucleotide) at the 3′-end. Such Cas9 variants are not restricted to target sequences that include the canonical PAM sequence at the 3′-end.

Among the known Cas proteins, Streptococcus pyogenes Cas9 has been mostly widely used as a tool for genome engineering.¹⁵ This Cas9 protein is a large, multi-domain protein containing two distinct nuclease domains. Point mutations can be introduced into Cas9 to abolish nuclease activity, resulting in a dead Cas9 (dCas9) that still retains its ability to bind DNA in a sgRNA-programmed manner.¹⁶In principle, such Cas9 variants, when fused to another protein or domain, can target that protein to virtually any DNA sequence simply by co-expression with an appropriate sgRNA. Thus, this disclosure also comtemplates fusion proteins comprising such Cas9 variants and a DNA modifying domain (e.g., a deaminase, a nuclease, a nickase, a recombinase, a methyltransferase, a methylase, an acetylase, an acetyltransferase, a transcriptional activator, or a transcriptional repressor domain), as well as the use of such fusion proteins in correcting mutations in a genome (e.g., the genome of a human subject) that are associated with disease, or generating mutations in a genome (e.g., the human genome) to decrease or prevent expression of a gene.

In some embodiments, any of the Cas9 proteins provided herein may be fused to a protein that has an enzymatic activity. In some embodiments, the enzymatic activity modifies a target DNA. In some embodiments, the enzymatic activity is nuclease activity, methyltransferase activity, demethylase activity, DNA repair activity, DNA damage activity, deamination activity, dismutase activity, alkylation activity, depurination activity, oxidation activity, pyrimidine dimer forming activity, integrase activity, transposase activity, recombinase activity, polymerase activity, ligase activity, helicase activity, photolyase activity or glycosylase activity. In some cases, the enzymatic activity is nuclease activity. In some cases, the nuclease activity introduces a double strand break in the target DNA. In some cases, the enzymatic activity modifies a target polypeptide associated with the target DNA. In some cases, the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity, deubiquitinating activity, adenylation activity, deadenylation activity, SUMOylating activity, deSUMOylating activity, ribosylation activity, deribosylation activity, myristoylation activity or demyristoylation activity. In some cases, the target polypeptide is a histone and the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity or deubiquitinating activity.

In some embodiments, any of the Cas9 proteins provided herein may be fused to a protein that has an enzymatic activity. In some embodiments, the enzymatic activity modifies a polypeptide associated with DNA (e.g. a histone). In some embodiments, the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity (i.e., ubiquitination activity), deubiquitinating activity, adenylation activity, deadenylation activity, SUMOylating activity, deSUMOylating activity, ribosylation activity, deribosylation activity, myristoylation activity, demyristoylation activity glycosylation activity (e.g., from O-GlcNAc transferase) or deglycosylation activity. The enzymatic activities listed herein catalyze covalent modifications to proteins. Such modifications are known in the art to alter the stability or activity of the target protein (e.g., phosphorylation due to kinase activity can stimulate or silence protein activity depending on the target protein). Of particular interest as protein targets are histones. Histone proteins are known in the art to bind DNA and form complexes known as nucleosomes. Histones can be modified (e.g., by methylation, acetylation, ubuitination, phosphorylation) to elicit structural changes in the surrounding DNA, thus controlling the accessibility of potentially large portions of DNA to interacting factors such as transcription factors, polymerases and the like. A single histone can be modified in many different ways and in many different combinations (e.g., trimethylation of lysine 27 of histone 3, H3K27, is associated with DNA regions of repressed transcription while trimethylation of lysine 4 of histone 3, H3K4, is associated with DNA regions of active transcription). Thus, a site-directed modifying polypeptide with histone-modifying activity finds use in the site specific control of DNA structure and can be used to alter the histone modification pattern in a selected region of target DNA. Such methods find use in both research and clinical applications.

In some embodiments, the deaminase domain catalyzes the removal of an amine group from a molecule. In further embodiments, cytidine deaminase domains deaminate cytosine to yield uracil. In other embodiments, the nuclease domain has enzymatic activity and may cleave phosphodiester bonds between the nucleotide subunits of nucleic acids. In some embodiments, recombinase domains, which recombine specific sequences of DNA, may be used to manipulate the structure of genomes and to control gene expression. In further embodiments, methylase domains may be utilized to methylate their respective substrates, while acetylase domains may be used to acetylate their respective substrates. In other embodiments, acetyltransferase domains may be used to transfer an acetyl group. Examples of acetyltransferase molecules include, but are not limited to, histone acetyltransferases (e.g., CBP histone acetyltransferase), choline acetyltransferase, chloramphenicol acetytransferase, serotonic N-acetyltransferase, NatA acetyltransferase, and NatB acetyltransferase. The disclosure also contemplates transcriptional activator and transcriptional repressor domains. Transcriptional activator domains are regions of a transcription factor which may activate transcription from a promoter through an interaction or multiple interactions with a DNA binding domain, general transcription factors, and RNA polymerase. Transcriptional repressor domins are regions of a transcription factor which may repress transcription from a protomer through an interaction or multiple interactions with a DNA binding domain, general transcription factors, and RNA polymerase.

The potential of the Cas9 system for genome engineering is immense. Its unique ability to bring proteins to specific sites in a genome programmed by the sgRNA can be developed into a variety of site-specific genome engineering tools beyond nucleases, including transcriptional activators, transcriptional repressors, histone-modifying proteins, integrases, deaminases, and recombinases.¹¹ Some of these potential applications have recently been implemented through dCas9 fusions with transcriptional activators to afford RNA-guided transcriptional activators,^(17,18) transcriptional repressors,^(16,19,20) and chromatin modification enzymes.²¹ Simple co-expression of these fusions with a variety of sgRNAs results in specific expression of the target genes. These seminal studies have paved the way for the design and construction of readily programmable sequence-specific effectors for the precise manipulation of genomes.

Some aspects of this disclosure provide strategies, systems, proteins, nucleic acids, compositions, cells, reagents, methods, and kits that are useful for the targeted binding, editing, and/or cleaving of nucleic acids, including editing a single site within a subject's genome, e.g., a human subject's genome. In some embodiments, recombinant Cas9 proteins are provided that comprise at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten mutations as compared to a naturally occurring Cas9 protein, and that exhibit activity on target sequences that do not include the canonical PAM (5′-NGG-3′, where N is any nucleotide) at the 3′-end. Examples of such Cas9 protein mutations are given in Tables 3, 5, 8, and 9. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzymatic domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid binding, editing, and/or cleaving are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid binding, editing, and/or cleaving proteins, e.g., fusion proteins of Cas9 variants and nucleic acid editing enzymes or domains, are provided.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of Cas9 as provided by any of the sequences set forth in SEQ ID NOs: 9-262, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of S. pyogenes Cas9 having the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the recombinant Cas9 protein comprises a RuvC and an HNH domain. In some embodiments, the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X294R, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of A262T, K294R, S409I, E480K, E543D, M694I, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Other aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of Cas9 as provided by any of the sequences set forth in SEQ ID NOs: 10-262, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262; and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. In some embodiments, the Cas9 protein comprises a RuvC and an HNH domain. In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X267G, X294R, X405I, X409I, X480K, X543D, X694I, X1219V, X1224K, and X1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of A262T, S267G, K294R, F405I, S409I, E480K, E543D, M694I, E1219V, N1224K, and Q1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in in SEQ ID NOs: 10-262.

It should be appreciated that any of the amino acid mutations described herein, (e.g., A262T) from a first amino acid residue (e.g., A) to a second amino acid residue (e.g., T) may also include mutations from the first amino acid residue to an amino acid residue that is similar to (e.g., conserved) the second amino acid residue. For example, a mutation of an alanine to a threonine (e.g., a A262T mutation) may also be a mutation from an alanine to an amino acid that is similar in size and chemical properties to a threonine, for example, serine. Additional similar amino acid pairs include, but are not limited to, the following: phenylalanine and tyrosine; asparagine and glutamine; methionine and cysteine; aspartic acid and glutamic acid; and arginine and lysine. The skilled artisan would recognize that such conservative amino acid substitutions will likely have minor effects on protein structure and are likely to be well tolerated without compromising function. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to a threonine may be an amino acid mutation to a serine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to an arginine may be an amino acid mutation to a lysine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to an isoleucine, may be an amino acid mutation to an alanine, valine, methionine, or leucine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to a lysine may be an amino acid mutation to an arginine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to an aspartic acid may be an amino acid mutation to a glutamic acid or asparigine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to a valine may be an amino acid mutation to an alanine, isoleucine, methionine, or leucine. In some embodiments, any amino of the amino acid mutations provided herein from one amino acid to a glycine may be an amino acid mutation to an alanine. It should be appreciated, however, that additional conserved amino acid residues would be recognized by the skilled artisan and any of the amino acid mutations to other conserved amino acid residues are also within the scope of this disclosure.

In some embodiments, the Cas9 protein is a Cas9 domain of a fusion protein. In some embodiments, the amino acid sequence of the Cas9 protein comprises an X1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In some embodiments, the mutation is X1219A, X1219I, X1219M, or X1219L.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an E1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the mutation is E1219A, E1219I, E1219M or E1219L.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X480K mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In some embodiments, the mutation is X480R.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an E480K mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the mutation is E480R.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X543D mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In some embodiments, the mutation is X543N.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an E543D mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the mutation is E543N.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X480K, X543D, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X262T, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X294R, X480K, X543D, X1219V, X1256K, and X1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X294R, X480K, X543D, X1219V, and X1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X267G, X294R, X480K, X543D, X1219V, X1224K, and X1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X262T, X405I, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations E480K, E543D, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations A262T, S409I, E480K, E543D, M694I, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations K294R, E480K, E543D, E1219V, Q1256K, and L1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations K294R, E480K, E543D, E1219V, and Q1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations S267G, K294R, E480K, E543D, E1219V, N1224K, and Q1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations A262T, F405I, S409I, E480K, E543D, M694I, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

The HNH nuclease domain of Cas9 functions to cleave the DNA strand complementary to the guide RNA (gRNA). Its active site consists of a ββα-metal fold, and its histidine 840 activates a water molecule to attack the scissile phosphate, which is more electrophilic due to coordination with a magnesium ion, resulting in cleavage of the the 3′-5′ phosphate bond. In some embodiments, the amino acid sequence of the HNH domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the HNH domain of any of SEQ ID NOs: 9-262. In some embodiments, the amino acid sequence of the HNH domain is identical to the amino acid sequence of the HNH domain of any of SEQ ID NOs: 9-262.

The RuvC domain of Cas9 cleaves the non-target DNA strand. It is encoded by sequentially disparate sites which interact in the tertiary structure to form the RuvC cleaveage domain and consists of an RNase H fold structure. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 9-262. In some embodiments, the amino acid sequence of the RuvC domain is identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 9-262.

In some embodiments, the Cas9 protein comprises one or more mutations that affects (e.g., inhibits) the ability of Cas9 to cleave one or both strands of a DNA duplex. In some embodiments, the Cas9 protein comprises a D10A and/or a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10X₁ and/or a H840X₂ mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X₁ is any amino acid except for D, and X₂ is any amino acid except for H. In some embodiments, the Cas9 protein comprises an D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an H at amino acid residue 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D at amino acid residue 10 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the Cas9 protein of the present disclosure exhibits activity, for example, increased binding, on a target sequence that does not include the canonical PAM sequence (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9 wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits increased activity on a target sequence that does not comprise the canonical PAM sequence (5′-NGG-3′) at its 3′ end as compared to Streptococcus pyogenes Cas9 as provided in SEQ ID NO: 9. In some embodiments, the Streptococcus pyogenes Cas9 comprises a RuvC and an HNH domain. In other embodiments, the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9

As one example, the Cas9 protein may exhibit increased binding to the target sequence, may exhibit increased nuclease activity at the target sequence, or may exhibit an increase in other activities, depending on whether the Cas 9 protein is fused to an additional domain, such as an enzyme that has enzymatic activity. In some embodiments, the enzymatic activity modifies a target DNA. In some embodiments, the enzymatic activity is nuclease activity, methyltransferase activity, demethylase activity, DNA repair activity, DNA damage activity, deamination activity, dismutase activity, alkylation activity, depurination activity, oxidation activity, pyrimidine dimer forming activity, integrase activity, transposase activity, recombinase activity, polymerase activity, ligase activity, helicase activity, photolyase activity or glycosylase activity. In some cases, the enzymatic activity is nuclease activity. In some cases, the nuclease activity introduces a double strand break in the target DNA. In some cases, the enzymatic activity modifies a target polypeptide associated with the target DNA. In some cases, the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity, deubiquitinating activity, adenylation activity, deadenylation activity, SUMOylating activity, deSUMOylating activity, ribosylation activity, deribosylation activity, myristoylation activity or demyristoylation activity. In some cases, the target polypeptide is a histone and the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity or deubiquitinating activity.

In some embodiments, any of the Cas9 protein is fused to a protein that has an enzymatic activity. In some embodiments, the enzymatic activity modifies a polypeptide associated with DNA (e.g. a histone). In some embodiments, the enzymatic activity is methyltransferase activity, demethylase activity, acetyltransferase activity, deacetylase activity, kinase activity, phosphatase activity, ubiquitin ligase activity (i.e., ubiquitination activity), deubiquitinating activity, adenylation activity, deadenylation activity, SUMOylating activity, deSUMOylating activity, ribosylation activity, deribosylation activity, myristoylation activity, demyristoylation activity glycosylation activity (e.g., from O-GlcNAc transferase) or deglycosylation activity. The enzymatic activities listed herein catalyze covalent modifications to proteins. Such modifications are known in the art to alter the stability or activity of the target protein (e.g., phosphorylation due to kinase activity can stimulate or silence protein activity depending on the target protein). Of particular interest as protein targets are histones. Histone proteins are known in the art to bind DNA and form complexes known as nucleosomes. Histones can be modified (e.g., by methylation, acetylation, ubuitination, phosphorylation) to elicit structural changes in the surrounding DNA, thus controlling the accessibility of potentially large portions of DNA to interacting factors such as transcription factors, polymerases and the like. A single histone can be modified in many different ways and in many different combinations (e.g., trimethylation of lysine 27 of histone 3, H3K27, is associated with DNA regions of repressed transcription while trimethylation of lysine 4 of histone 3, H3K4, is associated with DNA regions of active transcription). Thus, a site-directed modifying polypeptide with histone-modifying activity finds use in the site specific control of DNA structure and can be used to alter the histone modification pattern in a selected region of target DNA. Such methods find use in both research and clinical applications.

In some embodiments, the Cas9 protein exhibits activity on a target sequence having a 3′ end that is not directly adjacent to, or does not have the canonical PAM sequence (5′-NGG-3′), that is at least 2-fold, at least 3-fold, at least 5-fold, at least 10-fold, at least 50-fold, at least 100-fold, at least 500-fold, at least 1,000-fold, at least 5,000-fold, at least 10,000-fold, at least 50,000-fold, at least 100,000-fold, at least 500,000-fold, or at least 1,000,000-fold increased as compared to the activity of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9 on the same target sequence.

In some embodiments, the 3′-end of the target sequence is directly adjacent to an AGC, GAG, TTT, GTG, CAA CAC, GAT, TAA, ACG, CGA, or CGT sequence.

In some embodiments, the Cas9 protein activity is measured by a nuclease assay or a nucleic acid binding assay, which are known in the art and would be apparent to the skilled artisan. As provided herein, the Cas9 protein may be fused to one or more domains that confer an activity to the protein, such as a nucleic acid editing activity (e.g., deaminase activity or transcriptional activation activity), which may be measured (e.g., by a deaminase assay or transcriptional activation assay). In some embodiments, the Cas9 protein is fused to a deaminase domain and its activity may be measured using a deaminase assay. In some embodiments, the Cas9 protein is fused to a transcriptional activation domain and its activity may be measured using a transcriptional activation assay, for example, reporter activation assay where the reporter, e.g., GFP or luciferase, among others, is expressed in response to Cas9 binding to a target sequence.

In some embodiments, the amino acid sequence of the Cas9 protein comprises any of the mutations provided herein. For example, in some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X267G, X294R, X405I, X409I, X480K, X543D, X694I, X1219V, X1224K, X1256K, and X1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In other embodiments, the mutations may be A262T, S267G, K294R, F405I, S409I, E480K, E543D, M694I, E1219V, N1224K, Q1256K, and L1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises any of the mutations provided herein. For example, in some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X294R, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid. In other embodiments, the mutations may be A262T, K294R, S409I, E480K, E543D, M694I, or E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X1219V mutation or an E1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X480K mutation or an E480K mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X543D mutation or a E543D mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 comprises the mutations X480K, X543D, and X1219V; or the mutations E480K, E543D, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 comprises the mutations X262T, X409I, X480K, X543D, X694I, and X1219V; or the mutations A262T, S409I, E480K, E543D, M694I, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X294R, X480K, X543D, X1219V, X1256K, and X1362P; or the mutations K294R, E480K, E543D, E1219V, Q1256K, and L1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X294R, X480K, X543D, X1219V, and X1256K, or mutations K294R, E480K, E543D, E1219V, and Q1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X267G, X294R, X480K, X543D, X1219V, X1224K, and X1256K; or the mutations S267G, K294R, E480K, E543DE1219V, N1224K, and Q1256K of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the mutations X262T, X405I, X409I, X480K, X543D, X694I, and X1219V; or the mutations A262T, F405I, S409I, E480K, E543D, M694I, and E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.

In some embodiments, the amino acid sequence of the HNH domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the HNH domain of any of SEQ ID NOs: 9-262. In some embodiments, the amino acid sequence of the HNH domain is identical to the amino acid sequence of any of the HNH domains of SEQ ID NOs: 9-262.

In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 9-262. In some embodiments, the amino acid sequence of the RuvC domain is identical to the amino acid sequence of any of the RuvC domains of SEQ ID NOs: 9-262.

In some embodiments, the Cas9 protein comprises at D10A and/or a H840A mutation in the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10X₁ and/or a H840X₂ mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X₁ is any amino acid except for D, and wherein X₂ is any amino acid except for H. In some embodiments, the Cas9 protein comprises an D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an H at amino acid residue 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an D at amino acid residue 10 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Some aspects of this disclosure provide fusion proteins comprising a Cas9 protein as provided herein that is fused to a second protein, thus forming a fusion protein. In some embodiments, the second protein is fused to the N-terminus of the Cas9 protein. In some embodiments, the second protein is fused to the C-terminus of the Cas9 protein. In some embodiments, the Cas9 domain and the effector domain are fused via a linker. The linker may be as simple as a covalent bond, or it may be a polymeric linker many atoms in length. In certain embodiments, the linker is a polpeptide or based on amino acids. In other embodiments, the linker is not peptide-like. In certain embodiments, the linker is a covalent bond (e.g., a carbon-carbon bond, disulfide bond, carbon-heteroatom bond, etc.). In certain embodiments, the linker is a carbon-nitrogen bond of an amide linkage. In certain embodiments, the linker is a cyclic or acyclic, substituted or unsubstituted, branched or unbranched aliphatic or heteroaliphatic linker. In certain embodiments, the linker is polymeric (e.g., polyethylene, polyethylene glycol, polyamide, polyester, etc.). In certain embodiments, the linker comprises a monomer, dimer, or polymer of aminoalkanoic acid. In certain embodiments, the linker comprises an aminoalkanoic acid (e.g., glycine, ethanoic acid, alanine, beta-alanine, 3-aminopropanoic acid, 4-aminobutanoic acid, 5-pentanoic acid, etc.). In certain embodiments, the linker comprises a monomer, dimer, or polymer of aminohexanoic acid (Ahx). In certain embodiments, the linker is based on a carbocyclic moiety (e.g., cyclopentane, cyclohexane). In other embodiments, the linker comprises a polyethylene glycol moiety (PEG). In other embodiments, the linker comprises amino acids. In certain embodiments, the linker comprises a peptide. In certain embodiments, the linker comprises an aryl or heteroaryl moiety. In certain embodiments, the linker is based on a phenyl ring. The linker may included functionalized moieties to facilitate attachment of a nucleophile (e.g., thiol, amino) from the peptide to the linker. Any electrophile may be used as part of the linker. Exemplary electrophiles include, but are not limited to, activated esters, activated amides, Michael acceptors, alkyl halides, aryl halides, acyl halides, and isothiocyanates.

In some embodiments, the linker comprises a chemical group or molecule linking two molecules or moieties, e.g., two domains of a fusion protein, such as, for example, a nuclease-inactive Cas9 domain and a effector domain (e.g., a deaminase domain). In some embodiments, the linker comprises one or more amino acid residues. For example, the linker may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 25, 30, 35, 40, 45, 50, or more amino acid residues. In some embodiments, the linker is 3, 9, 16, or 21 amino acids in length. In some embodiments, the linker comprises a (GGGGS)_(n) (SEQ ID NO: 5), a (G)_(n), an (EAAAK)_(n) (SEQ ID NO: 6), a (GGS)_(n), an SGSETPGTSESATPES (SEQ ID NO: 7) (also referred to as XTEN), or an (XP)_(n) motif, or a combination of any of these, wherein n is independently an integer between 1 and 30. In some embodiments, wherein the linker comprises a (GGS)₃ motif or a SGSETPGTSESATPES (SEQ ID NO: 7) (XTEN) motif.

Some aspects of this disclosure provide fusion proteins comprising a Cas9 protein as provided herein that is fused to a second protein, thus forming a fusion protein. In some embodiments, the second protein is fused to the N-terminus of the Cas9 protein. In some embodiments, the second protein is fused to the C-terminus of the Cas9 protein. In some embodiments, the Cas9 domain and the effector domain are fused via a nuclear localization sequence (NLS), for example a NLS comprising the amino acid sequence PKKKRKV (SEQ ID NO: 299), MDSLLMNRRKFLYQFKNVRWAKGRRETYLC (SEQ ID NO: 300), or SPKKKRKVEAS (SEQ ID NO: 284). In some embodiments, a NLS may be combined with any of the linkers listed above.

In some embodiments, the effector domain comprises an enzymatic domain. In some embodiments, the effector domain comprises a nuclease, a nickase, a recombinase, a deaminase, a methyltransferase, a methylase, an acetylase, an acetyltransferase, a transcriptional activator, or a transcriptional repressor domain, which may have nuclease activity, nickase activity, recombinase activity, deaminase activity, methyltransferase activity, methylase activity, acetylase activity, acetyltransferase activity transcriptional activation activity or transcriptional repression activity, respectively. In some embodiments, the effector domain is a effector domain. In some embodiments, the effector domain is a deaminase domain. In some embodiments, the deaminase is a cytosine deaminase or a cytidine deaminase. In some embodiments, the deaminase is an apolipoprotein B mRNA-editing complex (APOBEC) family deaminase. In some embodiments, the deaminase is an APOBEC1 deaminase. In some embodiments, the deaminase is an APOBEC2 deaminase. In some embodiments, the deaminase is an APOBEC3 deaminase. In some embodiments, the deaminase is an APOBEC3A deaminase. In some embodiments, the deaminase is an APOBEC3D deaminase. In some embodiments, the deaminase is an APOBEC3E deaminase. In some embodiments, the deaminase is an APOBEC3F deaminase. In some embodiments, the deaminase is an APOBEC3G deaminase. In some embodiments, the deaminase is an APOBEC3H deaminase. In some embodiments, the deaminase is an APOBEC4 deaminase. In some embodiments, the deaminase is an activation-induced deaminase (AID). In some embodiments, the effector domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the deaminase domain of any one of SEQ ID NOs: 263-281. In some embodiments, the deaminase is a cytidine deaminase. In some embodiments, the deaminase is an apolipoprotein B mRNA-editing complex (APOBEC) family deaminase. In some embodiments, the deaminase is an APOBEC1 family deaminase. In some embodiments, the deaminase is an activation-induced cytidine deaminase (AID). In some embodiments, the deaminase is an ACF1/ASE deaminase. In some embodiments, the deaminase is an adenosine deaminase. In some embodiments, the deaminase is an ADAT family deaminase.

Some aspects of this disclosure provide fusion proteins comprising a Cas9 protein fused to a effector domain, e.g., a deaminase, and a uracil glycosylase inhibitor (UGI). Some aspects of this disclosure are based on the recognition that such fusion proteins may exhibit an increased nucleic acid editing efficiency as compared to fusion proteins not comprising an UGI domain. Domains such as the deaminase domains and UGI domains have been described and are within the scope of this disclosure. For example domains such as deaminase domains and UGI domains have been described in Provisional Application Nos. 62/245,828, filed Oct. 23, 2015, 62/279,346 filed Jan. 15, 2016, 62/311,763 filed Mar. 22, 2016, 62/322,178 filed Apr. 13, 2016, 62/357,352 filed Jun. 30, 2016, 62/370,700 filed Aug. 3, 2016, 62/398,490 filed Sep. 22, 2016, and 62/408,686 filed Oct. 14, 2016; the entire contents of each is incorporated by reference herein. It should be appreciated that the deaminase domains and UGI domains described in the foregoing references are within the scope of this disclosure and may be fused with any of the Cas9 proteisn provided herein.

In some embodiments, the effector domain of the fusion protein is a nuclease domain. In some embodiments, the nuclease domain is a FokI DNA cleavage domain. In some embodiments, the fusion protein dimerizes. In certain embodiments, the dimer of the fusion protein is active. For example, two Fokl DNA cleavage domains may dimerize to cleave a nucleic acid.

In some embodiments, the Cas9 protein is fused to a second Cas9 protein. In some embodiments, the second Cas9 protein is the Cas9 protein of any one of claims 1-345. In some embodiments, the second Cas9 protein is fused to the N-terminus of the fusion protein. In some embodiments, the second Cas9 protein is fused to the C-terminus of the fusion protein. In some embodiments, the Cas9 protein and the second Cas9 protein are fused via a second linker. In some embodiments, the second linker comprises a (GGGGS)_(n) (SEQ ID NO: 5), a (G)_(n), an (EAAAK)_(n)(SEQ ID NO: 6), a (GGS)_(n), an SGSETPGTSESATPES (SEQ ID NO: 7), or an (XP)_(n) motif, or a combination of any of these, wherein n is independently an integer between 1 and 30. In some embodiments, the second linker comprises a (GGS)₃ motif.

Some aspects of this disclosure provide complexes comprising a Cas9 protein, or a Cas9 fusion protein as provided herein, and a guide RNA bound to the Cas9 protein, or the Cas9 fusion protein.

In some embodiments, the target sequence is a DNA sequence. In some embodiments, the target sequence is a sequence in the genome of a mammal. In some embodiments, the target sequence is a sequence in the genome of a human. In some embodiments, the 3′-end of the target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′).

Some aspects of this disclosure provide methods of using the Cas9 proteins, fusion proteins, or complexes provided herein. For example, some aspects of this disclosure provide methods comprising contacting a DNA molecule (a) with a Cas9 protein or a fusion protein as provided herein and a guide RNA, wherein the guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a target sequence; or (b) with a Cas9 protein, a Cas9 fusion protein, or a Cas9 protein or fusion protein complex with a gRNA as provided herein. In some embodiments, the 3′-end of the target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′). In some embodiments, the 3′-end of the target sequence is immediately adjacent to an AGC, GAG, TTT, GTG, or CAA sequence. In some embodiments, the target DNA sequence comprises a sequence associated with a disease or disorder. In some embodiments, the target DNA sequence comprises a point mutation associated with a disease or disorder. In some embodiments, the activity of the Cas9 protein, the Cas9 fusion protein, or the complex results in correction of the point mutation. In some embodiments, the step of contacting is performed in vivo in a subject.

Some aspects of this disclosure provide kits comprising a nucleic acid construct, comprising (a) a nucleotide sequence encoding a Cas9 protein or a Cas9 fusion protein as provided herein; and (b) a heterologous promoter that drives expression of the sequence of (a). In some embodiments, the kit further comprises an expression construct encoding a guide RNA backbone, wherein the construct comprises a cloning site positioned to allow the cloning of a nucleic acid sequence identical or complementary to a target sequence into the guide RNA backbone.

Some aspects of this disclosure provide polynucleotides encoding any of the Cas9 proteins, Cas9 fusion proteins, or guide RNA bound to the Cas9 protein or Cas9 fusion protein provided herein. Some aspects of this disclosure provide vectors comprising such polynucleotides. In some embodiments, the vector comprises a heterologous promoter driving expression of the polynucleotide.

Some aspects of this disclosure provide cells comprising any of the Cas9 proteins, fusion proteins, nucleic acid molecules, and/or a vectors as provided herein.

The summary above is meant to illustrate, in a non-limiting manner, some of the embodiments, advantages, features, and uses of the technology disclosed herein. Other embodiments, advantages, features, and uses of the technology disclosed herein will be apparent from the Detailed Description, the Drawings, the Examples, and the Claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the activity of wild-type Streptococcus pyogenes Cas9 on canonical PAM libraries and non-canonical PAM libraries.

FIG. 2 shows the activity of exemplary evolved Cas9 clones on a PAM library after directed evolution.

FIG. 3 shows a comparison of wild-type and evolved Cas9 in a mammalian GFP activation assay.

FIGS. 4A to 4B show the binding activity of Cas9 (pJH306) and evolved Cas9 proteins with an 5′-NGG-3′ PAM sequence using GFP as a readout. On 5′-NGG-3′ PAMs, many of the evolved Cas9 proteins showed increased Cas9 binding activity relative to wild-type Cas9 based on an increase in GFP fluorescence signal. FIG. 4A is a graph representing Cas9 binding activity as a function of % cells above the background fluorescence. FIG. 4B is a graph showing Cas9 binding activity as a function of mean fluorescence. Cas9 proteins used in these experiments were dCas9 proteins fused to a VPR transcriptional activator.

FIGS. 5A to 5B show the binding activity of wild type dCas9-VPR (pJH306) and evolved dCas9-VPR proteins with an NNN PAM sequence using GFP as a readout. On a library of NNN PAMs, many of the evolved Cas9 proteins showed increased Cas9 binding activity relative to wild-type Cas9 based on an increase in GFP fluorescence signal. FIG. 5A is a graph representing Cas9 binding activity as a function of % cells above background fluorescence. FIG. 5B is a graph showing Cas9 binding activity as a function of mean fluorescence. Cas9 proteins used in these experiments were dCas9 proteins fused to VPR.

FIG. 6 shows dCas9-VPR on all 64 PAM sequences as demonstrated by mean fluorescence on transfected cells gated by iRFP fluorescence. WT dCas9-VPR is pJH306.

FIG. 7 shows in vitro cutting assay. On the gel, WT is wild-type Cas9 (SEQ ID NO: 9), and 1 is Cas9 (SEQ ID NO: 9) with the E1219V mutation.

FIG. 8 shows a Cas9 fusion protein that can be used to modulate PAM specificity. One possible configuration for a linked Cas9-dCas9 system that could be used for increasing Cas9 targeting to non-canonical PAMs is shown. dCas9 binding to 5′-NGG-3′ or another PAM could localize Cas9 to an area close to target 2. This localization could help Cas9 cut a previously inaccessible PAM.

FIGS. 9A to 9B show dCas9-VPR binding activity on the NNNNN PAM Library. FIG. 9A is a graph representing dCas9-VPR binding activity on the NNNNN PAM library as a function of the % cells above background fluorescence. FIG. 9B is a graph representing dCas9-VPR binding activity on the NNNNN PAM library as a function of mean fluorescence.

FIG. 10 shows Cas9 cutting activity using % of cells with GFP loss as a readout. Cas9 proteins were tested with two sgRNAs, which either targeted the canonical 5′-NGG-3′ PAM or a GAT PAM within a GFP gene.

FIG. 11 illustrates double-stranded DNA substrate bound by Cas9:DNA editing enzyme:sgRNA complexes. The DNA editing enzyme may be, without limitation, a nuclease, nickase, recombinase, deaminase, methyltransferase, methylase, acetylase, or acetyltransferase.

FIGS. 12A to 12D show the results of the PAM depletion assay. pJH760 was tested in the PAM depletion assay on four new targets: re2 (FIG. 12A), VEGF (FIG. 12B), CLTA (FIG. 12C), and CCR5D (FIG. 12D).

FIG. 13 shows GFP cutting in mammalian cells.

FIG. 14 shows the results of a PAM depletion assay to test pJH760 (xCas9 v1.0) on the re2 target.

FIG. 15 shows the results of a PAM depletion assay to test pJH760 (xCas9 v1.0) on the VEGF target.

FIG. 16 shows the results of a PAM depletion assay to test pJH760 (xCas9 v1.0) on the CLTA target.

FIG. 17 shows the results of a PAM depletion assay to test pJH760 (xCas9 v1.0) on the CCR5D target.

FIG. 18 shows the results of a PAM depletion assay to test four xCas9v3 mutants on the re2 target.

FIG. 19 shows the results of a PAM depletion assay to test four xCas9v3 mutants on the VEGF target.

FIG. 20 shows the results of a PAM depletion assay to test four xCas9v3 mutants on the CLTA target.

DEFINITIONS

As used herein and in the claims, the singular forms “a,” “an,” and “the” include the singular and the plural reference unless the context clearly indicates otherwise. Thus, for example, a reference to “an agent” includes a single agent and a plurality of such agents.

The term “Cas9” or “Cas9 nuclease” refers to an RNA-guided nuclease comprising a Cas9 protein, or a fragment thereof (e.g., a protein comprising an active or inactive DNA cleavage domain of Cas9, and/or the gRNA binding domain of Cas9). A Cas9 nuclease is also referred to sometimes as a casn1 nuclease or a CRISPR (clustered regularly interspaced short palindromic repeat)-associated nuclease. CRISPR is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and a Cas9 protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently, Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3′-5′ exonucleolytically. In nature, DNA-binding and cleavage typically requires protein and both RNAs. However, single guide RNAs (“sgRNA”, or simply “gNRA”) can be engineered so as to incorporate aspects of both the crRNA and tracrRNA into a single RNA species. See, e.g., Jinek M., Chylinski K., Fonfara I., Hauer M., Doudna J. A., Charpentier E. Science 337:816-821(2012), the entire contents of which are hereby incorporated by reference. Cas9 recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus non-self. Cas9 nuclease sequences and structures are well known to those of skill in the art (see, e.g., “Complete genome sequence of an M1 strain of Streptococcus pyogenes.” Ferretti et al., J. J., McShan W. M., Ajdic D. J., Savic D. J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A. N., Kenton S., Lai H. S., Lin S. P., Qian Y., Jia H. G., Najar F. Z., Ren Q., Zhu H., Song L., White J., Yuan X., Clifton S. W., Roe B. A., McLaughlin R. E., Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001); “CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III.” Deltcheva E., Chylinski K., Sharma C. M., Gonzales K., Chao Y., Pirzada Z. A., Eckert M. R., Vogel J., Charpentier E., Nature 471:602-607(2011); and “A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.” Jinek M., Chylinski K., Fonfara I., Hauer M., Doudna J. A., Charpentier E. Science 337:816-821(2012), the entire contents of each of which are incorporated herein by reference). Cas9 orthologs have been described in various species, including, but not limited to, S. pyogenes and S. thermophilus. Additional suitable Cas9 nucleases and sequences will be apparent to those of skill in the art based on this disclosure, and such Cas9 nucleases and sequences include Cas9 sequences from the organisms and loci disclosed in Chylinski, Rhun, and Charpentier, “The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems” (2013) RNA Biology 10:5, 726-737; the entire contents of which are incorporated herein by reference. In some embodiments, a Cas9 nuclease has an inactive (e.g., an inactivated) DNA cleavage domain.

A nuclease-inactivated Cas9 protein may interchangeably be referred to as a “dCas9” protein (for nuclease-“dead” Cas9). Methods for generating a Cas9 having an inactive DNA cleavage domain are known (See, e.g., Jinek et al., Science. 337:816-821(2012); Qi et al., “Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression” (2013) Cell. 28; 152(5):1173-83, the entire contents of each of which are incorporated herein by reference). For example, the DNA cleavage domain of Cas9 is known to include two subdomains, the HNH nuclease subdomain and the RuvC1 subdomain. The HNH subdomain cleaves the strand complementary to the gRNA, whereas the RuvC1 subdomain cleaves the non-complementary strand. Mutations within these subdomains can silence the nuclease activity of Cas9. For example, the mutations D10A and H840A completely inactivate the nuclease activity of S. pyogenes Cas9 (Jinek et al., Science. 337:816-821(2012); Qi et al., Cell. 28; 152(5):1173-83 (2013).

In some embodiments, proteins comprising fragments of Cas9 are provided. For example, in some embodiments, a protein comprises one of two Cas9 domains: (1) the gRNA binding domain of Cas9; or (2) the DNA cleavage domain of Cas9. In some embodiments, proteins comprising Cas9 or fragments thereof are referred to as “Cas9 variants.” A Cas9 variant shares homology to Cas9. For example a Cas9 variant is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to wild type Cas9. In some embodiments, the Cas9 variant comprises a fragment of Cas9 (e.g., a gRNA binding domain or a DNA-cleavage domain), such that the fragment is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to the corresponding fragment of wild type Cas9. In some embodiments, wild type Cas9 corresponds to Cas9 from Streptococcus pyogenes (NCBI Reference Sequence: NC_017053.1, SEQ ID NO:1 (nucleotide); SEQ ID NO:2 (amino acid)).

(SEQ ID NO: 1) ATGGATAAGAAATACTCAATAGGCTTAGATATCGGCACAAATAGCGTCG GATGGGCGGTGATCACTGATGATTATAAGGTTCCGTCTAAAAAGTTCAA GGTTCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGG GCTCTTTTATTTGGCAGTGGAGAGACAGCGGAAGCGACTCGTCTCAAAC GGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTTATCT ACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTT CATCGACTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAAC GTCATCCTATTTTTGGAAATATAGTAGATGAAGTTGCTTATCATGAGAA ATATCCAACTATCTATCATCTGCGAAAAAAATTGGCAGATTCTACTGAT AAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGT TTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATCCTGATAATAGTGA TGTGGACAAACTATTTATCCAGTTGGTACAAATCTACAATCAATTATTT GAAGAAAACCCTATTAACGCAAGTAGAGTAGATGCTAAAGCGATTCTTT CTGCACGATTGAGTAAATCAAGACGATTAGAAAATCTCATTGCTCAGCT CCCCGGTGAGAAGAGAAATGGCTTGTTTGGGAATCTCATTGCTTTGTCA TTGGGATTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATG CTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTT ATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAG AATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATAGTG AAATAACTAAGGCTCCCCTATCAGCTTCAATGATTAAGCGCTACGATGA ACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGACAACAACTT CCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATG CAGGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTAT CAAACCAATTTTAGAAAAAATGGATGGTACTGAGGAATTATTGGTGAAA CTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAACGGCT CTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAG ACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATTGAA AAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTG GCAATAGTCGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTAC CCCATGGAATTTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCA TTTATTGAACGCATGACAAACTTTGATAAAAATCTTCCAAATGAAAAAG TACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGA ATTGACAAAGGTCAAATATGTTACTGAGGGAATGCGAAAACCAGCATTT CTTTCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAA ATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAAAAAT AGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAAT GCTTCATTAGGCGCCTACCATGATTTGCTAAAAATTATTAAAGATAAAG ATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTTTT AACATTGACCTTATTTGAAGATAGGGGGATGATTGAGGAAAGACTTAAA ACATATGCTCACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTC GCCGTTATACTGGTTGGGGACGTTTGTCTCGAAAATTGATTAATGGTAT TAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGAT GGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATAGTTTGA CATTTAAAGAAGATATTCAAAAAGCACAGGTGTCTGGACAAGGCCATAG TTTACATGAACAGATTGCTAACTTAGCTGGCAGTCCTGCTATTAAAAAA GGTATTTTACAGACTGTAAAAATTGTTGATGAACTGGTCAAAGTAATGG GGCATAAGCCAGAAAATATCGTTATTGAAATGGCACGTGAAAATCAGAC AACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTATGAAACGAATCGAA GAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTGTTG AAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTACAAAA TGGAAGAGACATGTATGTGGACCAAGAATTAGATATTAATCGTTTAAGT GATTATGATGTCGATCACATTGTTCCACAAAGTTTCATTAAAGACGATT CAATAGACAATAAGGTACTAACGCGTTCTGATAAAAATCGTGGTAAATC GGATAACGTTCCAAGTGAAGAAGTAGTCAAAAAGATGAAAAACTATTGG AGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATAATT TAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTT TATCAAACGCCAATTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCA CAAATTTTGGATAGTCGCATGAATACTAAATACGATGAAAATGATAAAC TTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTCTGA CTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAATTAC CATCATGCCCATGATGCGTATCTAAATGCCGTCGTTGGAACTGCTTTGA TTAAGAAATATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAA AGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATAGGC AAAGCAACCGCAAAATATTTCTTTTACTCTAATATCATGAACTTCTTCA AAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCCCTCTAAT CGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGAT TTTGCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTGTCA AGAAAACAGAAGTACAGACAGGCGGATTCTCCAAGGAGTCAATTTTACC AAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGATCCA AAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCCTAG TGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGTTAA AGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAAT CCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACT TAATCATTAAACTACCTAAATATAGTCTTTTTGAGTTAGAAAACGGTCG TAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAATGAGCTG GCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATTATG AAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGT GGAGCAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGTGAA TTTTCTAAGCGTGTTATTTTAGCAGATGCCAATTTAGATAAAGTTCTTA GTGCATATAACAAACATAGAGACAAACCAATACGTGAACAAGCAGAAAA TATTATTCATTTATTTACGTTGACGAATCTTGGAGCTCCCGCTGCTTTT AAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAAG AAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGA AACACGCATTGATTTGAGTCAGCTAGGAGGTGACTGA (SEQ ID NO: 2) MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIG ALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFF HRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLF EENPINASRVDAKAILSARLSKSRRLENLIAQLPGEKRNGLFGNLIALS LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK NLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQS FIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN ASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEERLK TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKK GILQTVKIVDELVKVMGHKPENIVIEMARENQTTQKGQKNSRERMKRIE EGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLS DYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYW RQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVA QILDSRMNTKYDENDKLIREVKVITLKSKLVSDERKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG KATAKYFFYSNIMNFEKTEITLANGEIRKRPLIETNGETGEIVWDKGRD FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDP KKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKN PIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISE FSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAF KYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain) 

In some embodiments, wild type Cas9 corresponds to, or comprises SEQ ID NO:3 (nucleotide) and/or SEQ ID NO: 4 (amino acid):

(SEQ ID NO: 3) ATGGATAAAAAGTATTCTATTGGTTTAGACATCGGCACTAATTCCGTTG GATGGGCTGTCATAACCGATGAATACAAAGTACCTTCAAAGAAATTTAA GGTGTTGGGGAACACAGACCGTCATTCGATTAAAAAGAATCTTATCGGT GCCCTCCTATTCGATAGTGGCGAAACGGCAGAGGCGACTCGCCTGAAAC GAACCGCTCGGAGAAGGTATACACGTCGCAAGAACCGAATATGTTACTT ACAAGAAATTTTTAGCAATGAGATGGCCAAAGTTGACGATTCTTTCTTT CACCGTTTGGAAGAGTCCTTCCTTGTCGAAGAGGACAAGAAACATGAAC GGCACCCCATCTTTGGAAACATAGTAGATGAGGTGGCATATCATGAAAA GTACCCAACGATTTATCACCTCAGAAAAAAGCTAGTTGACTCAACTGAT AAAGCGGACCTGAGGTTAATCTACTTGGCTCTTGCCCATATGATAAAGT TCCGTGGGCACTTTCTCATTGAGGGTGATCTAAATCCGGACAACTCGGA TGTCGACAAACTGTTCATCCAGTTAGTACAAACCTATAATCAGTTGTTT GAAGAGAACCCTATAAATGCAAGTGGCGTGGATGCGAAGGCTATTCTTA GCGCCCGCCTCTCTAAATCCCGACGGCTAGAAAACCTGATCGCACAATT ACCCGGAGAGAAGAAAAATGGGTTGTTCGGTAACCTTATAGCGCTCTCA CTAGGCCTGACACCAAATTTTAAGTCGAACTTCGACTTAGCTGAAGATG CCAAATTGCAGCTTAGTAAGGACACGTACGATGACGATCTCGACAATCT ACTGGCACAAATTGGAGATCAGTATGCGGACTTATTTTTGGCTGCCAAA AACCTTAGCGATGCAATCCTCCTATCTGACATACTGAGAGTTAATACTG AGATTACCAAGGCGCCGTTATCCGCTTCAATGATCAAAAGGTACGATGA ACATCACCAAGACTTGACACTTCTCAAGGCCCTAGTCCGTCAGCAACTG CCTGAGAAATATAAGGAAATATTCTTTGATCAGTCGAAAAACGGGTACG CAGGTTATATTGACGGCGGAGCGAGTCAAGAGGAATTCTACAAGTTTAT CAAACCCATATTAGAGAAGATGGATGGGACGGAAGAGTTGCTTGTAAAA CTCAATCGCGAAGATCTACTGCGAAAGCAGCGGACTTTCGACAACGGTA GCATTCCACATCAAATCCACTTAGGCGAATTGCATGCTATACTTAGAAG GCAGGAGGATTTTTATCCGTTCCTCAAAGACAATCGTGAAAAGATTGAG AAAATCCTAACCTTTCGCATACCTTACTATGTGGGACCCCTGGCCCGAG GGAACTCTCGGTTCGCATGGATGACAAGAAAGTCCGAAGAAACGATTAC TCCATGGAATTTTGAGGAAGTTGTCGATAAAGGTGCGTCAGCTCAATCG TTCATCGAGAGGATGACCAACTTTGACAAGAATTTACCGAACGAAAAAG TATTGCCTAAGCACAGTTTACTTTACGAGTATTTCACAGTGTACAATGA ACTCACGAAAGTTAAGTATGTCACTGAGGGCATGCGTAAACCCGCCTTT CTAAGCGGAGAACAGAAGAAAGCAATAGTAGATCTGTTATTCAAGACCA ACCGCAAAGTGACAGTTAAGCAATTGAAAGAGGACTACTTTAAGAAAAT TGAATGCTTCGATTCTGTCGAGATCTCCGGGGTAGAAGATCGATTTAAT GCGTCACTTGGTACGTATCATGACCTCCTAAAGATAATTAAAGATAAGG ACTTCCTGGATAACGAAGAGAATGAAGATATCTTAGAAGATATAGTGTT GACTCTTACCCTCTTTGAAGATCGGGAAATGATTGAGGAAAGACTAAAA ACATACGCTCACCTGTTCGACGATAAGGTTATGAAACAGTTAAAGAGGC GTCGCTATACGGGCTGGGGACGATTGTCGCGGAAACTTATCAACGGGAT AAGAGACAAGCAAAGTGGTAAAACTATTCTCGATTTTCTAAAGAGCGAC GGCTTCGCCAATAGGAACTTTATGCAGCTGATCCATGATGACTCTTTAA CCTTCAAAGAGGATATACAAAAGGCACAGGTTTCCGGACAAGGGGACTC ATTGCACGAACATATTGCGAATCTTGCTGGTTCGCCAGCCATCAAAAAG GGCATACTCCAGACAGTCAAAGTAGTGGATGAGCTAGTTAAGGTCATGG GACGTCACAAACCGGAAAACATTGTAATCGAGATGGCACGCGAAAATCA AACGACTCAGAAGGGGCAAAAAAACAGTCGAGAGCGGATGAAGAGAATA GAAGAGGGTATTAAAGAACTGGGCAGCCAGATCTTAAAGGAGCATCCTG TGGAAAATACCCAATTGCAGAACGAGAAACTTTACCTCTATTACCTACA AAATGGAAGGGACATGTATGTTGATCAGGAACTGGACATAAACCGTTTA TCTGATTACGACGTCGATCACATTGTACCCCAATCCTTTTTGAAGGACG ATTCAATCGACAATAAAGTGCTTACACGCTCGGATAAGAACCGAGGGAA AAGTGACAATGTTCCAAGCGAGGAAGTCGTAAAGAAAATGAAGAACTAT TGGCGGCAGCTCCTAAATGCGAAACTGATAACGCAAAGAAAGTTCGATA ACTTAACTAAAGCTGAGAGGGGTGGCTTGTCTGAACTTGACAAGGCCGG ATTTATTAAACGTCAGCTCGTGGAAACCCGCCAAATCACAAAGCATGTT GCACAGATACTAGATTCCCGAATGAATACGAAATACGACGAGAACGATA AGCTGATTCGGGAAGTCAAAGTAATCACTTTAAAGTCAAAATTGGTGTC GGACTTCAGAAAGGATTTTCAATTCTATAAAGTTAGGGAGATAAATAAC TACCACCATGCGCACGACGCTTATCTTAATGCCGTCGTAGGGACCGCAC TCATTAAGAAATACCCGAAGCTAGAAAGTGAGTTTGTGTATGGTGATTA CAAAGTTTATGACGTCCGTAAGATGATCGCGAAAAGCGAACAGGAGATA GGCAAGGCTACAGCCAAATACTTCTTTTATTCTAACATTATGAATTTCT TTAAGACGGAAATCACTCTGGCAAACGGAGAGATACGCAAACGACCTTT AATTGAAACCAATGGGGAGACAGGTGAAATCGTATGGGATAAGGGCCGG GACTTCGCGACGGTGAGAAAAGTTTTGTCCATGCCCCAAGTCAACATAG TAAAGAAAACTGAGGTGCAGACCGGAGGGTTTTCAAAGGAATCGATTCT TCCAAAAAGGAATAGTGATAAGCTCATCGCTCGTAAAAAGGACTGGGAC CCGAAAAAGTACGGTGGCTTCGATAGCCCTACAGTTGCCTATTCTGTCC TAGTAGTGGCAAAAGTTGAGAAGGGAAAATCCAAGAAACTGAAGTCAGT CAAAGAATTATTGGGGATAACGATTATGGAGCGCTCGTCTTTTGAAAAG AACCCCATCGACTTCCTTGAGGCGAAAGGTTACAAGGAAGTAAAAAAGG ATCTCATAATTAAACTACCAAAGTATAGTCTGTTTGAGTTAGAAAATGG CCGAAAACGGATGTTGGCTAGCGCCGGAGAGCTTCAAAAGGGGAACGAA CTCGCACTACCGTCTAAATACGTGAATTTCCTGTATTTAGCGTCCCATT ACGAGAAGTTGAAAGGTTCACCTGAAGATAACGAACAGAAGCAACTTTT TGTTGAGCAGCACAAACATTATCTCGACGAAATCATAGAGCAAATTTCG GAATTCAGTAAGAGAGTCATCCTAGCTGATGCCAATCTGGACAAAGTAT TAAGCGCATACAACAAGCACAGGGATAAACCCATACGTGAGCAGGCGGA AAATATTATCCATTTGTTTACTCTTACCAACCTCGGCGCTCCAGCCGCA TTCAAGTATTTTGACACAACGATAGATCGCAAACGATACACTTCTACCA AGGAGGTGCTAGACGCGACACTGATTCACCAATCCATCACGGGATTATA TGAAACTCGGATAGATTTGTCACAGCTTGGGGGTGACGGATCCCCCAAG AAGAAGAGGAAAGTCTCGAGCGACTACAAAGACCATGACGGTGATTATA AAGATCATGACATCGATTACAAGGATGACGATGACAAGGCTGCAGGA (SEQ ID NO: 4) MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFF HRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLF EENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQS FIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN ASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLK TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNY WRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINN YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEK NPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain)

In some embodiments, wild type Cas9 corresponds to Cas9 from Streptococcus pyogenes (NCBI Reference Sequence: NC_002737.2, SEQ ID NO: 282 (nucleotide); and Uniport Reference Sequence: Q99ZW2, SEQ ID NO: 9 (amino acid).

(SEQ ID NO: 282) ATGGATAAGAAATACTCAATAGGCTTAGATATCGGCACAAATAGCGTCG GATGGGCGGTGATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAA GGTTCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGG GCTCTTTTATTTGACAGTGGAGAGACAGCGGAAGCGACTCGTCTCAAAC GGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTTATCT ACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTT CATCGACTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAAC GTCATCCTATTTTTGGAAATATAGTAGATGAAGTTGCTTATCATGAGAA ATATCCAACTATCTATCATCTGCGAAAAAAATTGGTAGATTCTACTGAT AAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGT TTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATCCTGATAATAGTGA TGTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTT GAAGAAAACCCTATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTT CTGCACGATTGAGTAAATCAAGACGATTAGAAAATCTCATTGCTCAGCT CCCCGGTGAGAAGAAAAATGGCTTATTTGGGAATCTCATTGCTTTGTCA TTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATG CTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTT ATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAG AATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATACTG AAATAACTAAGGCTCCCCTATCAGCTTCAATGATTAAACGCTACGATGA ACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGACAACAACTT CCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATG CAGGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTAT CAAACCAATTTTAGAAAAAATGGATGGTACTGAGGAATTATTGGTGAAA CTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAACGGCT CTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAG ACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATTGAA AAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTG GCAATAGTCGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTAC CCCATGGAATTTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCA TTTATTGAACGCATGACAAACTTTGATAAAAATCTTCCAAATGAAAAAG TACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGA ATTGACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTT CTTTCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAA ATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAAAAAT AGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAAT GCTTCATTAGGTACCTACCATGATTTGCTAAAAATTATTAAAGATAAAG ATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTTTT AACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAA ACATATGCTCACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTC GCCGTTATACTGGTTGGGGACGTTTGTCTCGAAAATTGATTAATGGTAT TAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGAT GGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATAGTTTGA CATTTAAAGAAGACATTCAAAAAGCACAAGTGTCTGGACAAGGCGATAG TTTACATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAAAAAA GGTATTTTACAGACTGTAAAAGTTGTTGATGAATTGGTCAAAGTAATGG GGCGGCATAAGCCAGAAAATATCGTTATTGAAATGGCACGTGAAAATCA GACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTATGAAACGAATC GAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTG TTGAAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCA AAATGGAAGAGACATGTATGTGGACCAAGAATTAGATATTAATCGTTTA AGTGATTATGATGTCGATCACATTGTTCCACAAAGTTTCCTTAAAGACG ATTCAATAGACAATAAGGTCTTAACGCGTTCTGATAAAAATCGTGGTAA ATCGGATAACGTTCCAAGTGAAGAAGTAGTCAAAAAGATGAAAAACTAT TGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATA ATTTAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGG TTTTATCAAACGCCAATTGGTTGAAACTCGCCAAATCACTAAGCATGTG GCACAAATTTTGGATAGTCGCATGAATACTAAATACGATGAAAATGATA AACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTC TGACTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAAT TACCATCATGCCCATGATGCGTATCTAAATGCCGTCGTTGGAACTGCTT TGATTAAGAAATATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTA TAAAGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATA GGCAAAGCAACCGCAAAATATTTCTTTTACTCTAATATCATGAACTTCT TCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCCCTCT AATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGA GATTTTGCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTG TCAAGAAAACAGAAGTACAGACAGGCGGATTCTCCAAGGAGTCAATTTT ACCAAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGAT CCAAAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCC TAGTGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGT TAAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAA AATCCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAG ACTTAATCATTAAACTACCTAAATATAGTCTTTTTGAGTTAGAAAACGG TCGTAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAATGAG CTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATT ATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTT TGTGGAGCAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGT GAATTTTCTAAGCGTGTTATTTTAGCAGATGCCAATTTAGATAAAGTTC TTAGTGCATATAACAAACATAGAGACAAACCAATACGTGAACAAGCAGA AAATATTATTCATTTATTTACGTTGACGAATCTTGGAGCTCCCGCTGCT TTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAA AAGAAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTA TGAAACACGCATTGATTTGAGTCAGCTAGGAGGTGACTGA (SEQ ID NO: 9) MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFF HRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLF EENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQS FIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN ASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLK TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNY WRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDERKDFQFYKVREINN YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIEINGETGEIVWDKGR DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEK NPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain)

In some embodiments, Cas9 refers to Cas9 from: Corynebacterium ulcerans (NCBI Refs: NC_015683.1, NC_017317.1); Corynebacterium diphtheria (NCBI Refs: NC_016782.1, NC_016786.1); Spiroplasma syrphidicola (NCBI Ref: NC_021284.1); Prevotella intermedia (NCBI Ref: NC_017861.1); Spiroplasma taiwanense (NCBI Ref: NC_021846.1); Streptococcus iniae (NCBI Ref: NC_021314.1); Belliella baltica (NCBI Ref: NC_018010.1); Psychroflexus torquisI (NCBI Ref: NC_018721.1); Streptococcus thermophilus (NCBI Ref: YP_820832.1); Listeria innocua (NCBI Ref: NP_472073.1); Campylobacter jejuni (NCBI Ref: YP_002344900.1); or Neisseria. meningitidis (NCBI Ref: YP_002342100.1) or to a Cas9 from any of the organisms listed in Example 3.

In some embodiments, dCas9 corresponds to, or comprises in part or in whole, a Cas9 amino acid sequence having one or more mutations that inactivate the Cas9 nuclease activity. For example, in some embodiments, a dCas9 domain comprises D10A and/or H840A mutation.

dCas9 (D10A and H840A): (SEQ ID NO: 8)  MDKK YSIGLA*IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGET AEATRLKRTARRRYTR RKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI*VDEVAYHEKYPTIYH*LRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVD*KLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRR LENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA*KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK* NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS*IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE*VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGE*QKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASL GTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWG*RLSRK

LQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAK

SDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHY LDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLINLGAPAAFKYFDTTIDRKRYTSTK EVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain)

In some embodiments, Cas9 corresponds to, or comprises in part or in whole, a Cas9 amino acid sequence having one or more mutations that alter Cas9's nuclease activity. In some embodiments, Cas9 may be a Cas9 nickase, which is a version of Cas9 that generates a single-stranded DNA break at a specific location based on a co-expressed gRNA-defined target sequence, rather than a double-strand DNA break. For example, in some embodiments, a Cas9 domain comprises D10A mutation (e.g., SEQ ID NO: 301) and/or an H840A mutation (e.g., SEQ ID NO: 302). Exemplary Cas9 nickases are shown below. However, it should be appreciated that additional Cas9 nickases that generate a single-stranded DNA break of a DNA duplex would be apparent to the skilled artisan and are within the scope of this disclosure.

Cas9 D10A nickase: (SEQ ID NO: 301) MDKKYSIGLAIGINSVGWAVITDEYKVPSKKEKVLGNTDRHSIKKNLIG ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFF HRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLF EENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQS FIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN ASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLK TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNY WRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINN YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEK NPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain) Cas9 H840Anickase: (SEQ ID NO: 302) MDKKYSIGLDIGINSVGWAVITDEYKVPSKKEKVLGNTDRHSIKKNLIG ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFF HRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLF EENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNEDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQS FIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAE LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN ASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLK TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL SDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNY WRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDERKDFQFYKVREINN YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIEINGETGEIVWDKGR DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEK NPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD (single underline: HNH domain; double underline: RuvC domain)

In other embodiments, dCas9 variants having mutations other than D10A and H840A are provided, which, e.g., result in nuclease-inactivated Cas9 (dCas9). Such mutations, by way of example, include other amino acid substitutions at D10 and H820, or other substitutions within the nuclease domains of Cas9 (e.g., substitutions in the HNH nuclease subdomain and/or the RuvC1 subdomain). In some embodiments, variants or homologues of dCas9 (e.g., variants of SEQ ID NO: 9) are provided which are at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% identical to SEQ ID NO: 9. In some embodiments, variants of dCas9 (e.g., variants of SEQ ID NO: 9) are provided having amino acid sequences which are shorter, or longer than SEQ ID NO: 9, by about 5 amino acids, by about 10 amino acids, by about 15 amino acids, by about 20 amino acids, by about 25 amino acids, by about 30 amino acids, by about 40 amino acids, by about 50 amino acids, by about 75 amino acids, by about 100 amino acids, or more.

In some embodiments, Cas9 fusion proteins as provided herein comprise the full-length amino acid sequence of a Cas9 protein, e.g., one of the sequences provided above. In other embodiments, however, fusion proteins as provided herein do not comprise a full-length Cas9 sequence, but only a fragment thereof. For example, in some embodiments, a Cas9 fusion protein provided herein comprises a Cas9 fragment, wherein the fragment binds crRNA and tracrRNA or a sgRNA, but does not comprise a functional nuclease domain, e.g., it comprises only a truncated version of a nuclease domain or no nuclease domain at all. Exemplary amino acid sequences of suitable Cas9 domains and Cas9 fragments are provided herein, and additional suitable sequences of Cas9 domains and Cas9 fragments will be apparent to those of skill in the art. In some embodiments, a Cas9 fragment is at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% identical, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% of the amino acid length of a corresponding wild type Cas9 protein. In some embodiments, a Cas9 fragment comprises at least at least 100 amino acids in length. In some embodiments, the Cas9 fragment is at least 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500, 1550, or at least 1600 amino acids of a corresponding wild type Cas9 protein. In some embodiments, the Cas9 fragment comprises an amino acid sequence that has at least 10, at least 15, at least 20, at least 30, at leat 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 350, at least 400, at least 500, at least 600, at least 700, at least 800, at least 900, at least 1000, at least 1100, or at least 1200 identical contiguous amino acid residues of a corresponding wild type Cas9 protein.

Cas9. In some embodiments, Cas9 refers to Cas9 from: Corynebacterium ulcerans (NCBI Refs: NC_015683.1, NC_017317.1); Corynebacterium diphtheria (NCBI Refs: NC_016782.1, NC_016786.1); Spiroplasma syrphidicola (NCBI Ref: NC_021284.1); Prevotella intermedia (NCBI Ref: NC_017861.1); Spiroplasma taiwanense (NCBI Ref: NC_021846.1); Streptococcus iniae (NCBI Ref: NC_021314.1); Belliella baltica (NCBI Ref: NC_018010.1); Psychroflexus torquisI (NCBI Ref: NC_018721.1); Streptococcus thermophilus (NCBI Ref: YP_820832.1); Listeria innocua (NCBI Ref: NP_472073.1); Campylobacter jejuni (NCBI Ref: YP_002344900.1); or Neisseria. meningitidis (NCBI Ref: YP_002342100.1).

The term “deaminase” or “deaminase domain,” as used herein, refers to a protein or enzyme that catalyzes a deamination reaction. In some embodiments, the deaminase or deaminase domain is a cytidine deaminase, catalyzing the hydrolytic deamination of cytidine or deoxycytidine to uridine or deoxyuridine, respectively. In some embodiments, the deaminase or deaminase domain is a cytosine deaminase, catalyzing the hydrolytic deamination of cytosine to uracil. In some embodiments, the deaminase or deaminase domain is a naturally-occurring deaminase from an organism, such as a human, chimpanzee, gorilla, monkey, cow, dog, rat, or mouse. In some embodiments, the deaminase or deaminase domain is a variant of a naturally-occuring deaminase from an organism, that does not occur in nature. For example, in some embodiments, the deaminase or deaminase domain is at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75% at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to a naturally-occuring deaminase from an organism.

The term “effective amount,” as used herein, refers to an amount of a biologically active agent that is sufficient to elicit a desired biological response. For example, in some embodiments, an effective amount of a nuclease may refer to the amount of the nuclease that is sufficient to induce cleavage of a target site specifically bound and cleaved by the nuclease. In some embodiments, an effective amount of a fusion protein provided herein, e.g., of a fusion protein comprising a nuclease-inactive Cas9 domain and a effector domain (e.g., a deaminase domain) may refer to the amount of the fusion protein that is sufficient to induce editing of a target site specifically bound and edited by the fusion protein. As will be appreciated by the skilled artisan, the effective amount of an agent, e.g., a fusion protein, a nuclease, a deaminase, a recombinase, a hybrid protein, a protein dimer, a complex of a protein (or protein dimer) and a polynucleotide, or a polynucleotide, may vary depending on various factors such as, for example, on the desired biological response, e.g., on the specific allele, genome, or target site to be edited; on the cell or tissue being targeted; and on the agent being used.

The term “immediately adjacent” as used in the context of two nucleic acid sequences refers to two sequences that directly abut each other as part of the same nucleic acid molecule and are not separated by one or more nucleotides. Accordingly, sequences are immediately adjacent, when the nucleotide at the 3′-end of one of the sequences is directly connected to nucleotide at the 5′-end of the other sequence via a phosphodiester bond.

The term “linker,” as used herein, refers to a chemical group or a molecule linking two molecules or moieties, e.g., two domains of a fusion protein, such as, for example, a nuclease-inactive Cas9 domain and a effector domain (e.g., a deaminase domain). In some embodiments, a linker joins a gRNA binding domain of an RNA-programmable nuclease, including a Cas9 nuclease domain, and the catalytic domain of a nucleic-acid editing protein. In some embodiments, a linker joins a dCas9 and a nucleic-acid editing protein. Typically, the linker is positioned between, or flanked by, two groups, molecules, or other moieties and connected to each one via a covalent bond, thus connecting the two. In some embodiments, the linker is an amino acid or a plurality of amino acids (e.g., a peptide or protein). In some embodiments, the linker is an organic molecule, group, polymer, or chemical moiety. In some embodiments, the linker is 5-100 amino acids in length, for example, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 30-35, 35-40, 40-45, 45-50, 50-60, 60-70, 70-80, 80-90, 90-100, 100-150, or 150-200 amino acids in length. Longer or shorter linkers are also contemplated.

The term “mutation,” as used herein, refers to a substitution of a residue within a sequence, e.g., a nucleic acid or amino acid sequence, with another residue, or a deletion or insertion of one or more residues within a sequence. Mutations are typically described herein by identifying the original residue followed by the position of the residue within the sequence and by the identity of the newly substituted residue. Various methods for making the amino acid substitutions (mutations) provided herein are well known in the art, and are provided by, for example, Green and Sambrook, Molecular Cloning: A Laboratory Manual (4^(th) ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2012)).

The terms “nucleic acid” and “nucleic acid molecule,” as used herein, refer to a compound comprising a nucleobase and an acidic moiety, e.g., a nucleoside, a nucleotide, or a polymer of nucleotides. Typically, polymeric nucleic acids, e.g., nucleic acid molecules comprising three or more nucleotides are linear molecules, in which adjacent nucleotides are linked to each other via a phosphodiester linkage. In some embodiments, “nucleic acid” refers to individual nucleic acid residues (e.g. nucleotides and/or nucleosides). In some embodiments, “nucleic acid” refers to an oligonucleotide chain comprising three or more individual nucleotide residues. As used herein, the terms “oligonucleotide” and “polynucleotide” can be used interchangeably to refer to a polymer of nucleotides (e.g., a string of at least three nucleotides). In some embodiments, “nucleic acid” encompasses RNA as well as single and/or double-stranded DNA. Nucleic acids may be naturally occurring, for example, in the context of a genome, a transcript, mRNA, tRNA, rRNA, siRNA, snRNA, a plasmid, cosmid, chromosome, chromatid, or other naturally occurring nucleic acid molecule. On the other hand, a nucleic acid molecule may be a non-naturally occurring molecule, e.g., a recombinant DNA or RNA, an artificial chromosome, an engineered genome, or fragment thereof, or a synthetic DNA, RNA, DNA/RNA hybrid, or including non-naturally occurring nucleotides or nucleosides. Furthermore, the terms “nucleic acid,” “DNA,” “RNA,” and/or similar terms include nucleic acid analogs, e.g., analogs having other than a phosphodiester backbone. Nucleic acids can be purified from natural sources, produced using recombinant expression systems and optionally purified, chemically synthesized, etc. Where appropriate, e.g., in the case of chemically synthesized molecules, nucleic acids can comprise nucleoside analogs such as analogs having chemically modified bases or sugars, and backbone modifications. A nucleic acid sequence is presented in the 5′ to 3′ direction unless otherwise indicated. In some embodiments, a nucleic acid is or comprises natural nucleosides (e.g. adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxyguanosine, and deoxycytidine); nucleoside analogs (e.g., 2-aminoadenosine, 2-thiothymidine, inosine, pyrrolo-pyrimidine, 3-methyl adenosine, 5-methylcytidine, 2-aminoadenosine, C5-bromouridine, C5-fluorouridine, C5-iodouridine, C5-propynyl-uridine, C5-propynyl-cytidine, C5-methylcytidine, 2-aminoadenosine, 7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine, 0(6)-methylguanine, and 2-thiocytidine); chemically modified bases; biologically modified bases (e.g., methylated bases); intercalated bases; modified sugars (e.g., 2′-fluororibose, ribose, 2′-deoxyribose, arabinose, and hexose); and/or modified phosphate groups (e.g., phosphorothioates and 5′-N-phosphoramidite linkages). In some embodiments, an RNA is an RNA associated with the Cas9 system. For example, the RNA may be a CRISPR RNA (crRNA), a trans-encoded small RNA (tracrRNA), a single guide RNA (sgRNA), or a guide RNA (gRNA).

The term “proliferative disease,” as used herein, refers to any disease in which cell or tissue homeostasis is disturbed in that a cell or cell population exhibits an abnormally elevated proliferation rate. Proliferative diseases include hyperproliferative diseases, such as pre-neoplastic hyperplastic conditions and neoplastic diseases. Neoplastic diseases are characterized by an abnormal proliferation of cells and include both benign and malignant neoplasias. Malignant neoplasia is also referred to as cancer.

The terms “protein,” “peptide,” and “polypeptide” are used interchangeably herein, and refer to a polymer of amino acid residues linked together by peptide (amide) bonds. The terms refer to a protein, peptide, or polypeptide of any size, structure, or function. Typically, a protein, peptide, or polypeptide will be at least three amino acids long. A protein, peptide, or polypeptide may refer to an individual protein or a collection of proteins. One or more of the amino acids in a protein, peptide, or polypeptide may be modified, for example, by the addition of a chemical entity such as a carbohydrate group, a hydroxyl group, a phosphate group, a farnesyl group, an isofarnesyl group, a fatty acid group, a linker for conjugation, functionalization, or other modification, etc. A protein, peptide, or polypeptide may also be a single molecule or may be a multi-molecular complex. A protein, peptide, or polypeptide may be just a fragment of a naturally occurring protein or peptide. A protein, peptide, or polypeptide may be naturally occurring, recombinant, or synthetic, or any combination thereof. The term “fusion protein” as used herein refers to a hybrid polypeptide which comprises protein domains from at least two different proteins. One protein may be located at the amino-terminal (N-terminal) portion of the fusion protein or at the carboxy-terminal (C-terminal) protein thus forming an “amino-terminal fusion protein” or a “carboxy-terminal fusion protein,” respectively. A protein may comprise different domains, for example, a nucleic acid binding domain (e.g., the gRNA binding domain of Cas9 that directs the binding of the protein to a target site) and a nucleic acid cleavage domain or a catalytic domain of a nucleic-acid editing protein. In some embodiments, a protein comprises a proteinaceous part, e.g., an amino acid sequence constituting a nucleic acid binding domain, and an organic compound, e.g., a compound that can act as a nucleic acid cleavage agent. In some embodiments, a protein is in a complex with, or is in association with, a nucleic acid, e.g., RNA. Any of the proteins provided herein may be produced by any method known in the art. For example, the proteins provided herein may be produced via recombinant protein expression and purification, which is especially suited for fusion proteins comprising a peptide linker. Methods for recombinant protein expression and purification are well known, and include those described by Green and Sambrook, Molecular Cloning: A Laboratory Manual (4^(th) ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2012)), the entire contents of which are incorporated herein by reference.

The term “RNA-programmable nuclease,” and “RNA-guided nuclease” are used interchangeably herein and refer to a nuclease that forms a complex with (e.g., binds or associates with) one or more RNA that is not a target for cleavage. In some embodiments, an RNA-programmable nuclease, when in a complex with an RNA, may be referred to as a nuclease:RNA complex. Typically, the bound RNA(s) is referred to as a guide RNA (gRNA). gRNAs can exist as a complex of two or more RNAs, or as a single RNA molecule. gRNAs that exist as a single RNA molecule may be referred to as single-guide RNAs (sgRNAs), though “gRNA” is used interchangeably to refer to guide RNAs that exist as either single molecules or as a complex of two or more molecules. Typically, gRNAs that exist as single RNA species comprise two domains: (1) a domain that shares homology to a target nucleic acid (e.g., and directs binding of a Cas9 complex to the target); and (2) a domain that binds a Cas9 protein. In some embodiments, domain (2) corresponds to a sequence known as a tracrRNA, and comprises a stem-loop structure. For example, in some embodiments, domain (2) is identical or homologous to a tracrRNA as provided in Jinek et al., Science 337:816-821(2012), the entire contents of which is incorporated herein by reference. Other examples of gRNAs (e.g., those including domain 2) can be found in U.S. Provisional Patent Application, U.S. Ser. No. 61/874,682, filed Sep. 6, 2013, entitled “Switchable Cas9 Nucleases and Uses Thereof,” and U.S. Provisional Patent Application, U.S. Ser. No. 61/874,746, filed Sep. 6, 2013, entitled “Delivery System For Functional Nucleases,” the entire contents of each are hereby incorporated by reference in their entirety. In some embodiments, a gRNA comprises two or more of domains (1) and (2), and may be referred to as an “extended gRNA.” For example, an extended gRNA will, e.g., bind two or more Cas9 proteins and bind a target nucleic acid at two or more distinct regions, as described herein. The gRNA comprises a nucleotide sequence that complements a target site, which mediates binding of the nuclease/RNA complex to said target site, providing the sequence specificity of the nuclease:RNA complex. In some embodiments, the RNA-programmable nuclease is the (CRISPR-associated system) Cas9 endonuclease, for example Cas9 (Csn1) from Streptococcus pyogenes (see, e.g., “Complete genome sequence of an M1 strain of Streptococcus pyogenes.” Ferretti J. J., McShan W. M., Ajdic D. J., Savic D. J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A. N., Kenton S., Lai H. S., Lin S. P., Qian Y., Jia H. G., Najar F. Z., Ren Q., Zhu H., Song L., White J., Yuan X., Clifton S. W., Roe B. A., McLaughlin R. E., Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663 (2001); “CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III.” Deltcheva E., Chylinski K., Sharma C. M., Gonzales K., Chao Y., Pirzada Z. A., Eckert M. R., Vogel J., Charpentier E., Nature 471:602-607 (2011); and “A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.” Jinek M., Chylinski K., Fonfara I., Hauer M., Doudna J. A., Charpentier E. Science 337:816-821 (2012), the entire contents of each of which are incorporated herein by reference.

Because RNA-programmable nucleases (e.g., Cas9) use RNA:DNA hybridization to target DNA cleavage sites, these proteins are able to be targeted, in principle, to any sequence specified by the guide RNA. Methods of using RNA-programmable nucleases, such as Cas9, for site-specific cleavage (e.g., to modify a genome) are known in the art (see e.g., Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823 (2013); Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013); Hwang, W. Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature biotechnology 31, 227-229 (2013); Jinek, M. et al. RNA-programmed genome editing in human cells. eLife 2, e00471 (2013); Dicarlo, J. E. et al. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic acids research (2013); Jiang, W. et al. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nature biotechnology 31, 233-239 (2013); the entire contents of each of which are incorporated herein by reference).

The term “subject,” as used herein, refers to an individual organism, for example, an individual mammal. In some embodiments, the subject is a human. In some embodiments, the subject is a non-human mammal. In some embodiments, the subject is a non-human primate. In some embodiments, the subject is a rodent. In some embodiments, the subject is a sheep, a goat, a cattle, a cat, or a dog. In some embodiments, the subject is a vertebrate, an amphibian, a reptile, a fish, an insect, a fly, or a nematode. In some embodiments, the subject is a research animal. In some embodiments, the subject is genetically engineered, e.g., a genetically engineered non-human subject. The subject may be of either sex, of any age, and at any stage of development.

The term “target site” refers to a sequence within a nucleic acid molecule that is deaminated by a deaminase or a fusion protein comprising a deaminase (e.g., a dCas9-deaminase fusion protein provided herein).

The terms “treatment,” “treat,” and “treating,” refer to a clinical intervention aimed to reverse, alleviate, delay the onset of, or inhibit the progress of a disease or disorder, or one or more symptoms thereof, as described herein. As used herein, the terms “treatment,” “treat,” and “treating” refer to a clinical intervention aimed to reverse, alleviate, delay the onset of, or inhibit the progress of a disease or disorder, or one or more symptoms thereof, as described herein. In some embodiments, treatment may be administered after one or more symptoms have developed and/or after a disease has been diagnosed. In other embodiments, treatment may be administered in the absence of symptoms, e.g., to prevent or delay onset of a symptom or inhibit onset or progression of a disease. For example, treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example, to prevent or delay their recurrence.

The term “recombinant” as used herein in the context of proteins or nucleic acids refers to proteins or nucleic acids that do not occur in nature, but are the product of human engineering. For example, in some embodiments, a recombinant protein or nucleic acid molecule comprises an amino acid or nucleotide sequence that comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations as compared to any naturally occurring sequence.

The term “nucleic acid editing enzyme” as used herein refers to proteins that are able to modify a nucleic acids or one or more nucleotide bases of a nucleic acid. For example, in some embodiments, a nucleic acid editing enzyme is a deaminase, which can catalyze C to T or G to A changes. Other suitable nucleid acid editing enzyme that may be used in accordance with this disclosure include, without limitation, a nuclease, nickase, recombinase, deaminase, methyltransferase, methylase, acetylase, or acetyltransferase.

DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS OF THE INVENTION

Some aspects of this disclosure provide recombinant Cas9 proteins that efficiently target DNA sequences that do not comprise the canonical PAM sequence (5′-NGG-3′, where N is any nucleotide, for example A, T, G, or C) at their 3′-ends. In some embodiments, the Cas9 proteins provided herein comprise one or more mutations identified in directed evolution experiments using a target sequence library comprising randomized PAM sequences. The recombinant non-PAM restricted Cas9 proteins provided herein are useful for targeting DNA sequences that do not comprise the canonical PAM sequence at their 3′-end and thus greatly extend the usefulness of Cas9 technology for gene editing.

Some aspects of this disclosure provide fusion proteins that comprise a Cas9 protein and an effector domain, for example, a DNA-editing domain, such as, e.g., a deaminase domain. The deamination of a nucleobase by a deaminase can lead to a point mutation at the specific residue, which is referred to herein as nucleic acid editing. Fusion proteins comprising a Cas9 protein or variant thereof and a DNA-editing domain can thus be used for the targeted editing of nucleic acid sequences. Such fusion proteins are useful for targeted editing of DNA in vitro, e.g., for the generation of mutant cells or animals; for the introduction of targeted mutations, e.g., for the correction of genetic defects in cells ex vivo, e.g., in cells obtained from a subject that are subsequently re-introduced into the same or another subject; and for the introduction of targeted mutations, e.g., the correction of genetic defects or the introduction of deactivating mutations in disease-associated genes in a subject in vivo. Typically, the Cas9 protein of the fusion proteins described herein does not have any nuclease activity but instead is a Cas9 fragment or a dCas9 protein. Methods for the use of Cas9 fusion proteins as described herein are also provided.

Non-limiting, exemplary nuclease-inactive Cas9 proteins are provided herein. One exemplary suitable nuclease-inactive Cas9 protein is the D10A/H840A Cas9 protein mutant:

MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKEKVLGNTDRHSIKKNLIGA LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLIP NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD LLFKTNRKVIVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ LKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDD SLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV MGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP VENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDD SIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI REVKVITLKSKLVSDERKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK YPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI TLANGEIRKRPLIEINGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEV QTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ SITGLYETRIDLSQLGGD (SEQ ID NO: 262; see, e.g., Qi et al., Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013; 152(5):1173-83, the entire contents of which are incorporated herein by reference).

Additional suitable nuclease-inactive Cas9 proteins will be apparent to those of skill in the art based on this disclosure. Such additional exemplary suitable nuclease-inactive Cas9 proteins include, but are not limited to, D10A, D839A, H840A, N863A, D10A/D839A, D10A/H840A, D10A/N863A, D839A/H840A, D839A/N863A, D10A/D839A/H840A, and D10A/D839A/H840A/N863A mutant proteins (See, e.g., Prashant et al., CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nature Biotechnology. 2013; 31(9): 833-838, the entire contents of which are incorporated herein by reference).

Recombinant Cas9 Proteins

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of Cas9 as provided by any of the sequences provided in SEQ ID NOs: 10-262, wherein the Cas9 protein comprises a RuvC and an HNH domain, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations at an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X294R, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid at the corresponding position.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of A262T, K294R, S409I, E480K, E543D, M694I, or E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an X1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an E1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the HNH domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the HNH domain of SEQ ID NO: 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of SEQ ID NO: 9. In some embodiments, the Cas9 protein comprises a D10A and/or a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Recombinant Cas9 Proteins with Activity on Non-Canonical PAMs

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits activity (e.g., increased activity) on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9.

In some embodiments, the Cas9 protein exhibits activity on a target sequence having a 3′-end that is not directly adjacent to the canonical PAM sequence (5′-NGG-3′) that is at least 2-fold, at least 3-fold, at least 5-fold, at least 10-fold, at least 50-fold, at least 100-fold, at least 500-fold, at least 1,000-fold, at least 5,000-fold, at least 10,000-fold, at least 50,000-fold, at least 100,000-fold, at least 500,000-fold, or at least 1,000,000-fold increased as compared to the activity of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9 on the same target sequence. In some embodiments, the 3′-end of the target sequence is directly adjacent to an AGC, GAG, TTT, GTG, or CAA sequence. In some embodiments, the Cas9 protein activity is measured by a nuclease assay, a deamination assay, or a transcriptional activation assay. In some embodiments, the transcriptional activation assay is a reporter activation assay, such as a GFP activation assay. Exemplary methods for measuring binding activity (e.g., of Cas9) using transcriptional activation assays are known in the art and would be apparent to the skilled artisan. For example, methods for measuring Cas9 activity using the tripartite activator VPR have been described in Chavez A., et al., “Highly efficient Cas9-mediated transcriptional programming.” Nature Methods 12, 326-328 (2015); the entire contents of which are incorporated by reference herein.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X294R, X409I, X480K, X543D, X694I, and X1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid at the corresponding position.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of A262T, K294R, S409I, E480K, E543D, M694I, or E1219V of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an X1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 comprises an X1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the HNH domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the HNH domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10A and/or a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits an increased activity on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the Cas9 protein comprises a D10A and a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits an increased activity on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the Cas9 protein comprises a D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 further comprises a histidine residue at position 840 as provided in SEQ ID NO: 9, or a corresponding histidine residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262. Without wishing to be bound by any particular theory, the presence of the catalytic residue H840 allows Cas9 to cleave the non-targeted strand, i.e., the strand bound by the sgRNA. In some embodiments, a Cas9 having an amino acid residue other than histidine at position 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding amino acid sequence provided in SEQ ID NOs: 10-262 may be changed or reverted such that amino acid residue 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding amino acid sequence provided in SEQ ID NOs: 10-262 is histidine.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9; wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, and 1256 of the amino acid sequence provided in SEQ ID NO: 9; wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein; and wherein the recombinant Cas9 protein exhibits activity (e.g., increased activity) on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9.

In some embodiments, the Cas9 protein exhibits an activity on a target sequence having a 3′-end that is not directly adjacent to the canonical PAM sequence (5′-NGG-3′) that is at least 2-fold, at least 3-fold, at least 5-fold, at least 10-fold, at least 50-fold, at least 100-fold, at least 500-fold, at least 1,000-fold, at least 5,000-fold, at least 10,000-fold, at least 50,000-fold, at least 100,000-fold, at least 500,000-fold, or at least 1,000,000-fold increased as compared to the activity of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9 on the same target sequence. In some embodiments, the 3′-end of the target sequence is directly adjacent to an AAA, AAC, AAG, AAT, CAA, CAC, CAG, CAT, GAA, GAC, GAG, GAT, TAA, TAC, TAG, TAT, ACA, ACC, ACG, ACT, CCA, CCC, CCG, CCT, GCA, GCC, GCG, GCT, TCA, TCC, TCG, TCT, AGA, AGC, AGT, CGA, CGC, CGT, GGA, GGC, GGT, TGA, TGC, TGT, ATA, ATC, ATG, ATT, CTA, CTC, CTG, CTT, GTA, GTC, GTG, GTT, TTA, TTC, TTG, or TTT PAM sequence.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of X262T, X267G, X294R, X405I, X409I, X480K, X543D, X694I, X1219V, X1224K, X1256K, and X1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid at the corresponding position.

In some embodiments, the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations selected from the group consisting of A262T, S267G, K294R, F405I, S409I, E480K, E543D, M694I, E1219V, N1224K, Q1256K, and L1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an X1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an E1219V mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X480K mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an E480K mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises an X543D mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises an E543D mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the Cas9 protein comprises the combination of mutations selected from the group consisting of (X480K, X543D, and X1219V); (X262T, X409I, X480K, X543D, X694I, and X1219V); (X294R, X480K, X543D, X1219V, X1256K, and X1362P); (X294R, X480K, X543D, X1219V, and X1256K); (X267G, X294R, X480K, X543D, X1219V, X1224K, and X1256K); and (X262T, X405I, X409I, X480K, X543D, X694I, and X1219V) of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the amino acid sequence of the Cas9 protein comprises the combination of mutations selected from the group consisting of (E480K, E543D, and E1219V); (A262T, S409I, E480K, E543D, M694I, and E1219V); (K294R, E480K, E543D, E1219V, Q1256K, and L1362P); (K294R, E480K, E543D, E1219V, and Q1256K); (S267G, K294R, E480K, E543DE1219V, N1224K, and Q1256K); and (A262T, F405I, S409I, E480K, E543D, M694I, and E1219V) of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

In some embodiments, the amino acid sequence of the HNH domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the HNH domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the Cas9 protein comprises a D10A and/or a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10A and an H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises an H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits increased activity on a target sequence that does not include the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the Cas9 protein comprises a D10A and a H840A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262.

Some aspects of this disclosure provide recombinant Cas9 proteins comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations at an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits increased activity on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9. In some embodiments, the amino acid sequence of the RuvC domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of the RuvC domain of any of SEQ ID NOs: 2, 4, or 9. In some embodiments, the Cas9 protein comprises a D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 further comprises a histidine residue at position 840 as provided in SEQ ID NO: 9, or a corresponding histidine residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262. Without wishing to be bound by any particular theory, the presence of the catalytic residue H840 allows Cas9 to cleave the non-targeted strand, i.e., the strand bound by the sgRNA. In some embodiments, a Cas9 having an amino acid residue other than histidine at position 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding amino acid sequence provided in SEQ ID NOs: 10-262 may be changed or reverted such that amino acid residue 840 of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding amino acid sequence provided in SEQ ID NOs: 10-262 is histidine.

Cas9 Fusion Proteins

Some aspects of this disclosure provide fusion proteins comprising a Cas9 protein as provided herein that is fused to a second protein, or a “fusion partner”, such as an effector domain, thus forming a fusion protein. In some embodiments, the effector domain is fused to the N-terminus of the Cas9 protein. In some embodiments, the effector domain is fused to the C-terminus of the Cas9 protein. In some embodiments, the Cas9 protein and the effector domain are fused to each other via a linker. Suitable strategies for generating fusion proteins according to aspects of this disclosure using linkers or without the use of linkers will also be apparent to those of skill in the art in view of the instant disclosure and the knowledge in the art. For example, Gilbert et al., CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013; 154(2):442-51, showed that C-terminal fusions of Cas9 with VP64 using 2 NLS's as a linker (SPKKKRKVEAS, SEQ ID NO: 284), can be employed for transcriptional activation. Mali et al., CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013; 31(9):833-8, reported that C-terminal fusions with VP64 without linker can be employed for transcriptional activation. And Maeder et al., CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013; 10: 977-979, reported that C-terminal fusions with VP64 using a Gly₄Ser (SEQ ID NO: 5) linker can be used as transcriptional activators. Recently, dCas9-FokI nuclease fusions have successfully been generated and exhibit improved enzymatic specificity as compared to the parental Cas9 enzyme (In Guilinger J P, Thompson D B, Liu D R. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat. Biotechnol. 2014; 32(6): 577-82, and in Tsai S Q, Wyvekens N, Khayter C, Foden J A, Thapar V, Reyon D, Goodwin M J, Aryee M J, Joung J K. Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014; 32(6):569-76. PMID: 24770325 a SGSETPGTSESATPES (SEQ ID NO: 7) or a GGGGS_(n) (SEQ ID NO: 5) linker was used in FokI-dCas9 fusion proteins, respectively). In some embodiments, the linker comprises a (GGGGS)_(n) (SEQ ID NO: 5), a (G)_(n), an (EAAAK)_(n) (SEQ ID NO: 6), a (GGS)_(n), an SGSETPGTSESATPES (SEQ ID NO: 7), or an (XP)_(n) motif, or a combination of any of these, wherein n is independently an integer between 1 and 30. In some embodiments, the effector domain comprises an enzymatic domain. Suiable effector domains include, without limitation a nuclease, nickase, recombinase, deaminase, methyltransferase, methylase, acetylase, acetyltransferase, transcriptional activator, and transcriptional repressor.

The linker may be as simple as a covalent bond, or it may be a polymeric linker many atoms in length. In certain embodiments, the linker is a polpeptide or based on amino acids. In other embodiments, the linker is not peptide-like. In certain embodiments, the linker is a covalent bond (e.g., a carbon-carbon bond, disulfide bond, carbon-heteroatom bond, etc.). In certain embodiments, the linker is a carbon-nitrogen bond of an amide linkage. In certain embodiments, the linker is a cyclic or acyclic, substituted or unsubstituted, branched or unbranched aliphatic or heteroaliphatic linker. In certain embodiments, the linker is polymeric (e.g., polyethylene, polyethylene glycol, polyamide, polyester, etc.). In certain embodiments, the linker comprises a monomer, dimer, or polymer of aminoalkanoic acid. In certain embodiments, the linker comprises an aminoalkanoic acid (e.g., glycine, ethanoic acid, alanine, beta-alanine, 3-aminopropanoic acid, 4-aminobutanoic acid, 5-pentanoic acid, etc.). In certain embodiments, the linker comprises a monomer, dimer, or polymer of aminohexanoic acid (Ahx). In certain embodiments, the linker is based on a carbocyclic moiety (e.g., cyclopentane, cyclohexane). In other embodiments, the linker comprises a polyethylene glycol moiety (PEG). In other embodiments, the linker comprises amino acids. In certain embodiments, the linker comprises a peptide. In certain embodiments, the linker comprises an aryl or heteroaryl moiety. In certain embodiments, the linker is based on a phenyl ring. The linker may included funtionalized moieties to facilitate attachment of a nucleophile (e.g., thiol, amino) from the peptide to the linker. Any electrophile may be used as part of the linker. Exemplary electrophiles include, but are not limited to, activated esters, activated amides, Michael acceptors, alkyl halides, aryl halides, acyl halides, and isothiocyanates.

In some embodiments, the effector domain comprises a effector enzyme. Suitable effector enzymes that may be used in accordance with this disclosure include nucleases, nickases, recombinases, and deaminases. However additional effector enzymes would be apparent to the skilled artisan and are within the scope of this disclosure. In other embodiments, the effector domain comprises a domain that modulates transcriptional activity. Such transcriptional modulating domains may be, without limitation, a transcriptional activator or transcriptional repressor domain.

In some embodiments, the effector domain is a effector domain. In some embodiments, the effector domain is a deaminase domain. In some embodiments, the deaminase is a cytosine deaminase or a cytidine deaminase. In some embodiments, the deaminase is an apolipoprotein B mRNA-editing complex (APOBEC) family deaminase. In some embodiments, the deaminase is an APOBEC1 deaminase. In some embodiments, the deaminase is an APOBEC2 deaminase. In some embodiments, the deaminase is an APOBEC3 deaminase. In some embodiments, the deaminase is an APOBEC3A deaminase. In some embodiments, the deaminase is an APOBEC3D deaminase. In some embodiments, the deaminase is an APOBEC3E deaminase. In some embodiments, the deaminase is an APOBEC3F deaminase. In some embodiments, the deaminase is an APOBEC3G deaminase. In some embodiments, the deaminase is an APOBEC3H deaminase. In some embodiments, the deaminase is an APOBEC4 deaminase. In some embodiments, the deaminase is an activation-induced deaminase (AID).

In some embodiments, the effector domain is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the deaminase domain of any one of SEQ ID NOs: 263-281.

In some embodiments, the effector domain is a nuclease domain. In some embodiments, the nuclease domain is a FokI DNA cleavage domain. In some embodiments, this disclosure provides dimers of the fusion proteins provided herein, e.g., dimers of fusion proteins may include a dimerizing nuclease domain.

In some embodiments, the Cas9 protein comprises an amino acid sequence that is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of Cas9 as provided by any of the sequences provided in SEQ ID NOs: 10-262, wherein the Cas9 protein comprises a RuvC and an HNH domain, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. In some embodiments, the Cas9 protein comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein, and wherein the recombinant Cas9 protein exhibits activity on a target sequence that does not comprise the canonical PAM (5′-NGG-3′) at its 3′-end as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9.

In some embodiments, the Cas9 protein comprises an amino acid sequence that is at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to the amino acid sequence of Cas9 as provided by any of the sequences provided in SEQ ID NOs: 10-262, wherein the Cas9 protein comprises a RuvC and an HNH domain, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. In some embodiments, the Cas9 protein comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9, comprising the RuvC and HNH domains of SEQ ID NO: 9, wherein the amino acid sequence of the Cas9 protein comprises at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, and 1256 of the amino acid sequence provided in SEQ ID NO: 9; wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein; and wherein the recombinant Cas9 protein exhibits increased activity on a target sequence that does not include the canonical PAM (5′-NGG-3′) at its 3′-end, as compared to Streptococcus pyogenes Cas9 as provided by SEQ ID NO: 9.

Some aspects of this disclosure provide fusion proteins comprising (i) a nuclease-inactive Cas9 protein; and (ii) a effector domain. In some embodiments, the effector domain is a DNA-editing domain. In some embodiments, the effector domain possesses deaminase activity. In some embodiments, the effector domain comprises or is a deaminase domain. In some embodiments, the deaminase is a cytidine deaminase. In some embodiments, the deaminase is an apolipoprotein B mRNA-editing complex (APOBEC) family deaminase. In some embodiments, the deaminase is an APOBEC1 family deaminase. In some embodiments, the deaminase is an activation-induced cytidine deaminase (AID). Some nucleic-acid editing domains as well as Cas9 fusion proteins including such domains are described in detail herein. Additional suitable effector domains will be apparent to the skilled artisan based on this disclosure. In some embodiments, the nucleid-acid editing domain is a FokI nuclease domain.

The instant disclosure provides Cas9:effector domain fusion proteins of various configurations. In some embodiments, the effector domain is fused to the N-terminus of the Cas9 protein. In some embodiments, the effector domain is fused to the C-terminus of the Cas9 protein. In some embodiments, the Cas9 protein and the effector domain are fused via a linker. In some embodiments, the linker comprises (GGGGS)_(n) (SEQ ID NO: 5), (G)_(n), (EAAAK)_(n) (SEQ ID NO: 6), (GGS)_(n), or SGSETPGTSESATPES (SEQ ID NO: 7) motif (see, e.g., Guilinger J P, Thompson D B, Liu D R. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat. Biotechnol. 2014; 32(6): 577-82; the entire contents are incorporated herein by reference), or an (XP)_(n) motif, or a combination of any of these, wherein n is independently an integer between 1 and 30. In some embodiments, n is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30, or, if more than one linker or more than one linker motif is present, any combination thereof. Additional suitable linker motifs and linker configurations will be apparent to those of skill in the art. In some embodiments, suitable linker motifs and configurations include those described in Chen et al., Fusion protein linkers: property, design and functionality. Adv Drug Deliv Rev. 2013; 65(10):1357-69, the entire contents of which are incorporated herein by reference. Additional suitable linker sequences will be apparent to those of skill in the art based on the instant disclosure and knowledge in the art.

In some embodiments, the general architecture of exemplary Cas9 fusion proteins provided herein comprises the structure:

[NH₂]-[effector domain]-[Cas9]-[COOH] or

[NH₂]-[Cas9]-[effector domain]-[COOH],

wherein NH₂ is the N-terminus of the fusion protein, and COOH is the C-terminus of the fusion protein. FIG. 11 provides a schematic representation of a Cas9 protein fused to an effector domain (e.g., rAPOBEC 1) in complex with sgRNA and bound to the target nucleic acid sequence.

In some embodiments, any of the fusion proteins provided herein may comprise one or more nuclear localization sequence (NLS). As used herein, a nuclear localization sequence refers to an amino acid sequence that promotes importation of a protein, for example any of the fusion proteins provided herein having an NLS, into the cell nucleus (e.g., via nuclear transport). Typically, an NLS comprises one or more short amino acid sequences of positively charged lysines or arginines exposed on the protein surface. Nuclear localization sequences are known in the art and would be apparent to the skilled artisan. For example nuclear localization sequences have been described in Kalderon D., et al., “A short amino acid sequence able to specify nuclear location”. Cell (1984) 39 (3 Pt 2): 499-509; Dingwall C., et al., “The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen”. J Cell Biol. (1988) 107 (3): 841-9; Makkerh J. P., et al., “Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids”. Curr Biol. (1996) 6 (8): 1025-7; and Ray M., et al., “Quantitative tracking of protein trafficking to the nucleus using cytosolic protein delivery by nanoparticle-stabilized nanocapsules”. Bioconjug. Chem. (2015) 26 (6): 1004-7; the entire contents of each are incorporated by reference herein. Additional nuclear localization sequences are described, for example, in Plank et al., PCT/EP2000/011690, the entire contents are incorporated by reference herein. In some embodiments, a NLS comprises the amino acid sequence PKKKRKV (SEQ ID NO: 299) or MDSLLMNRRKFLYQFKNVRWAKGRRETYLC (SEQ ID NO: 300).

Exemplary features that may be present are localization sequences, such as nuclear localization sequences, cytoplasmic localization sequences, export sequences, such as nuclear export sequences, or other localization sequences, as well as sequence tags that are useful for solubilization, purification, or detection of the fusion proteins. Suitable localization signal sequences and sequences of protein tags are provided herein, and include, but are not limited to, biotin carboxylase carrier protein (BCCP) tags, myc-tags, calmodulin-tags, FLAG-tags, hemagglutinin (HA)-tags, polyhistidine tags, also referred to as histidine tags or His-tags, maltose binding protein (MBP)-tags, nus-tags, glutathione-S-transferase (GST)-tags, green fluorescent protein (GFP)-tags, thioredoxin-tags, S-tags, Softags (e.g., Softag 1, Softag 3), strep-tags, biotin ligase tags, FlAsH tags, V5 tags, and SBP-tags. Additional suitable sequences will be apparent to those of skill in the art and are within the scope of this disclosure.

Any of the nuclear localization sequences provided herein may be fused to the fusion protein in any suitable localization. For example, to promote translocation of the fusion protein into a cell nucleus without compromising function of the fusion protein. In some embodiments, the NLS is fused N-terminal to the Cas9 protein of the fusion protein. In some embodiments, the NLS is fused C-terminal to the Cas9 protein of the fusion protein. In some embodiments, the NLS is fused N-terminal to the effector domain of the fusion protein. In some embodiments, the NLS is fused C-terminal to the effector domain of the fusion protein.

In some embodiments, the effector domain is a deaminase. For example, in some embodiments, the general architecture of exemplary Cas9 fusion proteins with a deaminase domain comprises the structure:

[NH₂]-[NLS]-[Cas9]-[deaminase]-[COOH],

[NH₂]-[NLS]-[deaminase]-[Cas9]-[COOH],

[NH₂]-[Cas9]-[NLS]-[deaminase]-[COOH],

[NH₂]-[deaminase]-[NLS]-[Cas9]-[COOH],

[NH₂]-[deaminase]-[Cas9]-[NLS]-[COOH], or

[NH₂]-[Cas9]-[deaminase]-[NLS]-[COOH],

wherein NLS is a nuclear localization signal, NH₂ is the N-terminus of the fusion protein, and COOH is the C-terminus of the fusion protein. In some embodiments, a linker is inserted between the Cas9 protein and the deaminase domain. In some embodiments, any of the “]-[” may be one or more linkers. In some embodiments, the NLS is located C-terminal of the deaminase and/or the Cas9 domain. In some embodiments, the NLS is located between the deaminase and the Cas9 domain. Additional features, such as sequence tags, may also be present.

One exemplary suitable type of effector domain includes cytosine deaminases, for example, of the APOBEC family. The apolipoprotein B mRNA-editing complex (APOBEC) family of cytosine deaminase enzymes encompasses eleven proteins that serve to initiate mutagenesis in a controlled and beneficial manner.²⁹ One family member, activation-induced cytidine deaminase (AID), is responsible for the maturation of antibodies by converting cytosines in ssDNA to uracils in a transcription-dependent, strand-biased fashion.³⁰ The apolipoprotein B editing complex 3 (APOBEC3) enzyme provides protection to human cells against a certain HIV-1 strain via the deamination of cytosines in reverse-transcribed viral ssDNA.³¹ These proteins all require a Zn²⁺-coordinating motif (His-X-Glu-X₂₃₋₂₆-Pro-Cys-X₂₄-Cys; SEQ ID NO: 283) and bound water molecule for catalytic activity. The Glu residue acts to activate the water molecule to a zinc hydroxide for nucleophilic attack in the deamination reaction. Each family member preferentially deaminates at its own particular “hotspot,” ranging from WRC (W is A or T, R is A or G) for hAID, to TTC for hAPOBEC3F.³² A recent crystal structure of the catalytic domain of APOBEC3G revealed a secondary structure comprised of a five-stranded (3-sheet core flanked by six α-helices, which is believed to be conserved across the entire family.³³ The active center loops have been shown to be responsible for both ssDNA binding and in determining “hotspot” identity.³⁴ Overexpression of these enzymes has been linked to genomic instability and cancer, thus highlighting the importance of sequence-specific targeting.³⁵

Some aspects of this disclosure provide a systematic series of fusions between Cas9 and deaminase domains, e.g., cytosine deaminase enzymes such as APOBEC enzymes, or adenosine deaminase enzymes such as ADAT enzymes, that has been generated in order to direct the enzymatic activities of these deaminases to a specific site in genomic DNA. The advantages of using Cas9 as the recognition agent are two-fold: (1) the sequence specificity of Cas9 can be easily altered by simply changing the sgRNA sequence; and (2) Cas9 binds to its target sequence by denaturing the dsDNA, resulting in a stretch of DNA that is single-stranded and therefore a viable substrate for the deaminase. It will be understood that other catalytic domains, or catalytic domains from other deaminases, can also be used to generate fusion proteins with Cas9, and that the disclosure is not limited in this regard.

Some aspects of this disclosure are based on the recognition that cas9:deaminase fusion proteins can efficiently deaminate nucleotides at positions 3-11 according to the numbering scheme in FIG. 11. It should be appreciated that a person of skill in the art will be able to design suitable guide RNAs to target the fusion proteins to a target sequence that comprises a nucleotide to be deaminated. Both PAM-dependent Cas9 proteins or Cas9 proteins that can target PAM-less target sequences as provided herein, can be employed for deamination of a target nucleotide.

Some exemplary suitable nucleic-acid editing domains, e.g., deaminases and deaminase domains, that can be fused to Cas9 domains according to aspects of this disclosure are provided below. Typically, deaminase require a Zn₂₊-coordinating motif (His-X-Glu-X₂₃₋₂₆-Pro-Cys-X₂₄-Cys; SEQ ID NO: 283) and bound water molecule for catalytic activity. The Glu residue acts to activate the water molecule to a zinc hydroxide for nucleophilic attack in the deamination reaction. It will be understood that, in some embodiments, the active domain of the respective sequence can be used, e.g., the domain without a localizing signal (nuclear localizing signal, without nuclear export signal, cytoplasmic localizing signal).

Human AID: (SEQ ID NO: 263) MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYL RNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFL RGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYC WNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTL GL (underline: nuclear localization signal; double underline: nuclear export signal)  Mouse AID: (SEQ ID NO: 264) MDSLLMKQKKFLYHFKNVRWAKGRHETYLCYVVKRRDSATSCSLDFGHL RNKSGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVAEFL RWNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIGIMTFKDYFYC WNTFVENRERTFKAWEGLHENSVRLTRQLRRILLPLYEVDDLRDAFRML GF (underline: nuclear localization signal; double underline: nuclear export signal) Dog AID: (SEQ ID NO: 265) MDSLLMKQRKFLYHFKNVRWAKGRHETYLCYVVKRRDSATSFSLDFGHL RNKSGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFL RGYPNLSLRIFAARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYC WNTFVENREKTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTL GL (underline: nuclear localization signal; double underline: nuclear export signal) Bovine AID: (SEQ ID NO: 266) MDSLLKKQRQFLYQFKNVRWAKGRHETYLCYVVKRRDSPTSFSLDFGHL RNKAGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFL RGYPNLSLRIFTARLYFCDKERKAEPEGLRRLHRAGVQIAIMTFKDYFY CWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRT LGL (underline: nuclear localization signal; double underline: nuclear export signal) Mouse APOBEC-3: (SEQ ID NO: 267) MGPFCLGCSHRKCYSPIRNLISQETFKFHFKNLGYAKGRKDTFLCYEVT RKDCDSPVSLHHGVFKNKDNIHAEICFLYWFHDKVLKVLSPREEFKITW YMSWSPCFECAEQIVRFLATHHNLSLDIFSSRLYNVQDPETQQNLCRLV QEGAQVAAMDLYEFKKCWKKFVDNGGRRFRPWKRLLTNFRYQDSKLQEI LRPCYIPVPSSSSSTLSNICLTKGLPETRFCVEGRRMDPLSEEEFYSQF YNQRVKHLCYYHRMKPYLCYQLEQFNGQAPLKGCLLSEKGKQHAEILFL DKIRSMELSQVTITCYLTWSPCPNCAWQLAAFKRDRPDLILHIYTSRLY FHWKRPFQKGLCSLWQSGILVDVMDLPQFTDCWTNFVNPKRPFWPWKGL EIISRRTQRRLRRIKESWGLQDLVNDFGNLQLGPPMS (italic: nucleic acid editing domain) Rat APOBEC-3: (SEQ ID NO: 268) MGPFCLGCSHRKCYSPIRNLISQETFKFHFKNLRYAIDRKDTFLCYEVT RKDCDSPVSLHHGVFKNKDNIHAEICFLYWFHDKVLKVLSPREEFKITW YMSWSPCFECAEQVLRFLATHHNLSLDIFSSRLYNIRDPENQQNLCRLV QEGAQVAAMDLYEFKKCWKKFVDNGGRRFRPWKKLLTNFRYQDSKLQEI LRPCYIPVPSSSSSTLSNICLTKGLPETRFCVERRRVHLLSEEEFYSQF YNQRVKHLCYYHGVKPYLCYQLEQFNGQAPLKGCLLSEKGKQHAEILFL DKIRSMELSQVIITCYLTWSPCPNCAWQLAAFKRDRPDLILHIYTSRLY FHWKRPFQKGLCSLWQSGILVDVMDLPQFTDCWTNFVNPKRPFWPWKGL EIISRRTQRRLHRIKESWGLQDLVNDFGNLQLGPPMS (italic: nucleic acid editing domain) Rhesus macaque APOBEC-3G: (SEQ ID NO: 269) MVEPMDPRTFVSNFNNRPILSGLNTVWLCCEVKTKDPSGPPLDAKIFQG KVYSKAKYHPEM RFLRWFHKWRQLHHDQEYKVTWYVSWSPCTRCANSVA TFLAKDPKVTLTIFVARLYYFWKPDYQQALRILCQKRGGPHATMKIMNY NEFQDCWNKFVDGRGKPFKPRNNLPKHYTLLQATLGELLRHLMDPGTFT SNFNNKPWVSGQHETYLCYKVERLHNDTWVPLNQHRGFLRNQAPNIFIG FPKGRHAELCFLDHPFWKLDGQQYRVTCFTSWSPCFSCAQEMAKFISNN EHVSLCIFAARIYDDQGRYQEGLRALHRDGAKIAMMNYSEFEYCWDTFV DRQGRPFQPWDGLDEHSQALSGRLRAI (italic: nucleic acid editing domain; underline: cytoplasmic localization signal) Chimpanzee APOBEC-3G: (SEQ ID NO: 270) MKPHFRNPVERMYQDTFSDNFYNRPILSHRNTVWLCYEVKTKGPSRPPL DAKIFRGQVYSKLKYHPEAIRFFHWFSKWRKLHRDQEYEVTWYISWSPC TKCTRDVATFLAEDPKVTLTIFVARLYYFWDPDYQEALRSLCQKRDGPR ATMKIMNYDEFQHCWSKFVYSQRELFEPWNNLPKYYILLHIMLGEILRH SMDPPTFTSNFNNELWVRGRHETYLCYEVERLHNDTWVLLNQRRGFLCN QAPHKHGFLEGRHAELCFLDVIPFWKLDLHQDYRVTCFTSWSPCFSCAQ EMAKFISNNKHVSLCIFAARIYDDQGRCQEGLRTLAKAGAKISIMTYSE FKHCWDTFVDHQGCPFQPWDGLEEHSQALSGRLRAILQNQGN (italic: nucleic acid editing domain; underline: cytoplasmic localization signal) Green monkey APOBEC-3G: (SEQ ID NO: 271) MNPQIRNMVEQMEPDIFVYYFNNRPILSGRNTVWLCYEVKTKDPSGPPL DANIFQGKLYPEAKDHPEMKFLHWFRKWRQLHRDQEYEVTWYVSWSPCT RCANSVATFLAEDPKVTLTIFVARLYYFWKPDYQQALRILCQERGGPHA TMKIMNYNEFQHCWNEFVDGQGKPFKPRKNLPKHYTLLHATLGELLRHV MDPGTFTSNFNNKPWVSGQRETYLCYKVERSHNDTWVLLNQHRGFLRNQ APDRHGFPKGRHAELCFLDHPFWKLDDQQYRVTCFTSWSPCFSCAQKMA KFISNNKHVSLCIFAARIYDDQGRCQEGLRTLHRDGAKIAVMNYSEFEY CWDTFVDRQGRPFQPWDGLDEHSQALSGRLRAI (italic: nucleic acid editing domain; underline: cytoplasmic localization signal) Human APOBEC-3G: (SEQ ID NO: 272) MKPHFRNTVERMYRDTFSYNFYNRPILSRRNTVWLCYEVKTKGPSRPPL DAKIFRGQVYSELKYHPEMRFFHWFSKWRKLHRDQEYEVTWYISWSPCT KCTRDMATFLAEDPKVTLTIFVARLYYFWDPDYQEALRSLCQKRDGPRA TMKIMNYDEFQHCWSKFVYSQRELFEPWNNLPKYYILLHIMLGEILRHS MDPPTFTFNFNNEPWVRGRHETYLCYEVERMHNDTWVLLNQRRGFLCNQ APHKHGFLEGRHAELCFLDVIPFWKLDLDQDYRVTCFTSWSPCFSCAQE MAKFISKNKHVSLCIFTARIYDDQGRCQEGLRTLAEAGAKISIMTYSEF KHCWDTFVDHQGCPFQPWDGLDEHSQDLSGRLRAILQNQEN (italic: nucleic acid editing domain; underline: cytoplasmic localization signal) Human APOBEC-3F: (SEQ ID NO: 273) MKPHFRNTVERMYRDTFSYNFYNRPILSRRNTVWLCYEVKTKGPSRPRL DAKIFRGQVYSQPEHHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCPD CVAKLAEFLAEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVKIM DDEEFAYCWENFVYSEGQPFMPWYKFDDNYAFLHRTLKEILRNPMEAMY PHIFYFHFKNLRKAYGRNESWLCFTMEVVKHHSPVSWKRGVFRNQVDPE THCHAERCFLSWFCDDILSPNTNYEVTWYTSWSPCPECAGEVAEFLARH SNVNLTIFTARLYYFWDTDYQEGLRSLSQEGASVEIIVIGYKDFKYCWE NFVYNDDEPFKPWKGLKYNFLFLDSKLQEILE (italic: nucleic acid editing domain) Human APOBEC-3B: (SEQ ID NO: 274) MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLL WDTGVFRGQVYFKPQYHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCP DCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVTI MDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPD TFTFNFNNDPLVLRRRQTYLCYEVERLDNGTWVLMDQHMGFLCNEAKNL LCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVR AFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIIVITYDEF EYCWDTFVYRQGCPFQPWDGLEEHSQALSGRLRAILQNQGN (italic: nucleic acid editing domain) Human APOBEC-3C: (SEQ ID NO: 275) MNPQIRNPMKAMYPGTFYFQFKNLWEANDRNETWLCFTVEGIKRRSVVS WKTGVFRNQVDSETHCHAERCFLSWFCDDILSPNTKYQVTWYTSWSPCP DCAGEVAEFLARHSNVNLTIFTARLYYFQYPCYQEGLRSLSQEGVAVEI MDYEDFKYCWENFVYNDNEPFKPWKGLKTNFRLLKRRLRESLQ (italic: nucleic acid editing domain) Human APOBEC-3A: (SEQ ID NO: 276) MEASPASGPRHLMDPHIFTSNFNNGIGREIKTYLCYEVERLDNGTSVKM DQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFIS WSPCFSWGCAGEVRAFLENTHVRLRIFAARIYDYDPLYKEALQMLRDAG AQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQN QGN (italic: nucleic acid editing domain) Human APOBEC-3H: (SEQ ID NO: 277) MALLTAETFRLQFNNKRRLRRPYYPRKALLCYQLTPQNGSTPTRGYFEN KKKCHAEICFINEIKSMGLDETQCYQVTCYLTWSPCSSCAWELVDFIKA HDHLNLGIFASRLYYHWKPQQKGLRLLCGSQVPVEVMGFPKFADCWENF VDHEKPLSFNPYKMLEELDKNSRAIKRRLERIKIPGVRAQGRYMDILCD AEV (italic: nucleic acid editing domain) Human APOBEC-3D: (SEQ ID NO: 278) MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLL WDTGVFRGPVLPKRQSNHRQEVYFRFENHAEMCFLSWFCGNRLPANRRF QITWFVSWNPCLPCVVKVTKFLAEHPNVTLTISAARLYYYRDRDWRWVL LRLHKAGARVKIMDYEDFAYCWENFVCNEGQPFMPWYKFDDNYASLHRT LKEILRNPMEAMYPHIFYFHPKNLLKACGRNESWLCFTMEVTKHHSAVF RKRGVFRNQVDPETHCHAERCFLSWFCDDILSPNTNYEVTWYTSWSPCP ECAGEVAEFLARHSNVNLTIFTARLCYFWDTDYQEGLCSLSQEGASVKI IVIGYKDFVSCWKNFVYSDDEPFKPWKGLQTNFRLLKRRLREILQ (italic: nucleic acid editing domain) Human APOBEC-1: (SEQ ID NO: 279) MTSEKGPSTGDPTLRRRIEPWEFDVFYDPRELRKEACLLYEIKWGMSRK IWRSSGKNTTNHVEVNFIKKFTSERDFHPSMSCSITWFLSWSPCWECSQ AIREFLSRHPGVTLVIYVARLFWHMDQQNRQGLRDLVNSGVTIQIMRAS EYYHCWRNFVNYPPGDEAHWPQYPPLWM MLYALELHCIlLSLPPCLKI SRRWQNHLTFFRLHLQNCHYQTIPPHILLATGLIHPSVAWR Mouse APOBEC-1: (SEQ ID NO: 280) MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHS VWRHTSQNTSNHVEVNFLEKFTTERYFRPNTRCSITWFLSWSPCGECSR AITEFLSRHPYVTLFIYIARLYHHTDQRNRQGLRDLISSGVTIQIIVIT EQEYCYCWRNFVNYPPSNEAYWPRYPHLWVKLYVLELYCIILGLPPCLK ILRRKQPQLTFFTITLQTCHYQRIPPHLLWATGLK Rat APOBEC-1: (SEQ ID NO: 281) MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHS IWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSR AITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIIVIT EQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLN ILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK

In some embodiments, fusion proteins as provided herein comprise the full-length amino acid of a effector domain, e.g., one of the sequences provided above. In other embodiments, however, fusion proteins as provided herein do not comprise a full-length sequence of a effector domain, but only a fragment thereof. For example, in some embodiments, a fusion protein provided herein comprises a Cas9 protein and a fragment of a effector domain, e.g., wherein the fragment comprises a effector domain. Exemplary amino acid sequences of effector domains are shown in the sequences above as italicized letters, and additional suitable sequences of such domains will be apparent to those of skill in the art.

Additional suitable nucleic-acid editing domain, e.g., deaminase domain sequences, that can be used according to aspects of this disclosure, e.g., that can be fused to a nuclease-inactive Cas9 protein, will be apparent to those of skill in the art based on this disclosure. In some embodiments, such additional domain sequences include deaminase domain sequences that are at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% similar to the sequences provided herein. Additional suitable Cas9 proteins, variants, and sequences will also be apparent to those of skill in the art. Examples of such additional suitable Cas9 proteins include, but are not limited to Cas9 proteins with the following mutations: D10A, D10A/D839A/H840A, and D10A/D839A/H840A/N863A (See, e.g., Prashant et al., CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nature Biotechnology. 2013; 31(9): 833-838 the entire contents of which are incorporated herein by reference).

Additional suitable strategies for generating fusion proteins comprising a Cas9 protein and an effector domain, such as a DNA-editing domain, will be apparent to those of skill in the art based on this disclosure in combination with the general knowledge in the art. Suitable strategies for generating fusion proteins according to aspects of this disclosure using linkers or without the use of linkers will also be apparent to those of skill in the art in view of the instant disclosure and the knowledge in the art. For example, Gilbert et al., CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013; 154(2):442-51, showed that C-terminal fusions of Cas9 with VP64 using 2 NLS's as a linker (SPKKKRKVEAS, SEQ ID NO: 284), can be employed for transcriptional activation. Mali et al., Cas9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013; 31(9):833-8, reported that C-terminal fusions with VP64 without linker can be employed for transcriptional activation. And Maeder et al., CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013; 10: 977-979, reported that C-terminal fusions with VP64 using a Gly₄Ser (SEQ ID NO: 5) linker can be used as transcriptional activators. Recently, dCas9-FokI nuclease fusions have successfully been generated and exhibit improved enzymatic specificity as compared to the parental Cas9 enzyme (In Guilinger J P, Thompson D B, Liu D R. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat. Biotechnol. 2014; 32(6):577-82, and in Tsai S Q, Wyvekens N, Khayter C, Foden J A, Thapar V, Reyon D, Goodwin M J, Aryee M J, Joung J K. Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat. Biotechnol. 2014; 32(6):569-76. PMID: 24770325 a SGSETPGTSESATPES (SEQ ID NO: 7) or a GGGGS (SEQ ID NO: 5) linker was used in FokI-dCas9 fusion proteins, respectively).

In some embodiments, the Cas9 fusion protein comprises: (i) Cas9 protein; and (ii) a transcriptional activator domain. In some embodiments, the transcriptional activator domain comprises a VPR. VPR is a VP64-SV40-P65-RTA tripartite activator. In some embodiments, VPR comprises a VP64 amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 292:

(SEQ ID NO: 292) GAGGCCAGCGGTTCCGGACGGGCTGACGCATTGGACGATTTTGATCTGGA TATGCTGGGAAGTGACGCCCTCGATGATTTTGACCTTGACATGCTTGGTT CGGATGCCCTTGATGACTTTGACCTCGACATGCTCGGCAGTGACGCCCTT GATGATTTCGACCTGGACATGCTGATTAACTCTAGATAG

In some embodiments, VPR comprises a VP64 amino acid sequence as set forth in SEQ ID NO: 293:

(SEQ ID NO: 293) EASGSGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDAL DDFDLDMLINSR

In some embodiments, VPR comprises a VP64-SV40-P65-RTA amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 294:

(SEQ ID NO: 294) TCGCCAGGGATCCGTCGACTTGACGCGTTGATATCAACAAGTTTGTACAA AAAAGCAGGCTACAAAGAGGCCAGCGGTTCCGGACGGGCTGACGCATTGG ACGATTTTGATCTGGATATGCTGGGAAGTGACGCCCTCGATGATTTTGAC CTTGACATGCTTGGTTCGGATGCCCTTGATGACTTTGACCTCGACATGCT CGGCAGTGACGCCCTTGATGATTTCGACCTGGACATGCTGATTAACTCTA GAAGTTCCGGATCTCCGAAAAAGAAACGCAAAGTTGGTAGCCAGTACCTG CCCGACACCGACGACCGGCACCGGATCGAGGAAAAGCGGAAGCGGACCTA CGAGACATTCAAGAGCATCATGAAGAAGTCCCCCTTCAGCGGCCCCACCG ACCCTAGACCTCCACCTAGAAGAATCGCCGTGCCCAGCAGATCCAGCGCC AGCGTGCCAAAACCTGCCCCCCAGCCTTACCCCTTCACCAGCAGCCTGAG CACCATCAACTACGACGAGTTCCCTACCATGGTGTTCCCCAGCGGCCAGA TCTCTCAGGCCTCTGCTCTGGCTCCAGCCCCTCCTCAGGTGCTGCCTCAG GCTCCTGCTCCTGCACCAGCTCCAGCCATGGTGTCTGCACTGGCTCAGGC ACCAGCACCCGTGCCTGTGCTGGCTCCTGGACCTCCACAGGCTGTGGCTC CACCAGCCCCTAAACCTACACAGGCCGGCGAGGGCACACTGTCTGAAGCT CTGCTGCAGCTGCAGTTCGACGACGAGGATCTGGGAGCCCTGCTGGGAAA CAGCACCGATCCTGCCGTGTTCACCGACCTGGCCAGCGTGGACAACAGCG AGTTCCAGCAGCTGCTGAACCAGGGCATCCCTGTGGCCCCTCACACCACC GAGCCCATGCTGATGGAATACCCCGAGGCCATCACCCGGCTCGTGACAGG CGCTCAGAGGCCTCCTGATCCAGCTCCTGCCCCTCTGGGAGCACCAGGCC TGCCTAATGGACTGCTGTCTGGCGACGAGGACTTCAGCTCTATCGCCGAT ATGGATTTCTCAGCCTTGCTGGGCTCTGGCAGCGGCAGCCGGGATTCCAG GGAAGGGATGTTTTTGCCGAAGCCTGAGGCCGGCTCCGCTATTAGTGACG TGTTTGAGGGCCGCGAGGTGTGCCAGCCAAAACGAATCCGGCCATTTCAT CCTCCAGGAAGTCCATGGGCCAACCGCCCACTCCCCGCCAGCCTCGCACC AACACCAACCGGTCCAGTACATGAGCCAGTCGGGTCACTGACCCCGGCAC CAGTCCCTCAGCCACTGGATCCAGCGCCCGCAGTGACTCCCGAGGCCAGT CACCTGTTGGAGGATCCCGATGAAGAGACGAGCCAGGCTGTCAAAGCCCT TCGGGAGATGGCCGATACTGTGATTCCCCAGAAGGAAGAGGCTGCAATCT GTGGCCAAATGGACCTTTCCCATCCGCCCCCAAGGGGCCATCTGGATGAG CTGACAACCACACTTGAGTCCATGACCGAGGATCTGAACCTGGACTCACC CCTGACCCCGGAATTGAACGAGATTCTGGATACCTTCCTGAACGACGAGT GCCTCTTGCATGCCATGCATATCAGCACAGGACTGTCCATCTTCGACACA TCTCTGTTTTGA

In some embodiments, VPR comprises a VP64-SV40-P65-RTA amino acid sequence as set forth in SEQ ID NO: 295:

(SEQ ID NO: 295) SPGIRRLDALISTSLYKKAGYKEASGSGRADALDDFDLDMLGSDALDDFD LDMLGSDALDDFDLDMLGSDALDDFDLDMLINSRSSGSPKKKRKVGSQYL PDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSA SVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQ APAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEA LLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTT EPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIAD MDFSALLGSGSGSRDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFH PPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEAS HLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDE LTTTLESMTEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDT SLF

Some aspects of this disclosure provide fusion proteins comprising a transcription activator. In some embodiments, the transcriptional activator is VPR. In some embodiments, the VPR comprises a wild type VPR or a VPR as set forth in SEQ ID NO: 293. In some embodiments, the VPR proteins provided herein include fragments of VPR and proteins homologous to a VPR or a VPR fragment. For example, in some embodiments, a VPR comprises a fragment of the amino acid sequence set forth in SEQ ID NO: 293. In some embodiments, a VPR comprises an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 293 or an amino acid sequence homologous to a fragment of the amino acid sequence set forth in SEQ ID NO: 293. In some embodiments, proteins comprising VPR or fragments of VPR or homologs of VPR or VPR fragments are referred to as “VPR variants.” A VPR variant shares homology to VPR, or a fragment thereof. For example a VPR variant is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to a wild type VPR or a VPR as set forth in SEQ ID NO: 293. In some embodiments, the VPR variant comprises a fragment of VPR, such that the fragment is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to the corresponding fragment of wild type VPR or a VPR as set forth in SEQ ID NO: 293. In some embodiments, the VPR comprises the amino acid sequence set forth in SEQ ID NO: 293. In some embodiments, the VPR comprises an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO: 292. In some embodiments, a VPR is a VP64-SV40-P65-RTA triple activator. In some embodiments, the VP64-SV40-P65-RTA comprises a VP64-SV40-P65-RTA as set forth in SEQ ID NO: 295. In some embodiments, the VP64-SV40-P65-RTA proteins provided herein include fragments of VP64-SV40-P65-RTA and proteins homologous to a VP64-SV40-P65-RTA or a VP64-SV40-P65-RTA fragment. For example, in some embodiments, a VP64-SV40-P65-RTA comprises a fragment of the amino acid sequence set forth in SEQ ID NO: 295. In some embodiments, a VP64-SV40-P65-RTA comprises an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 295 or an amino acid sequence homologous to a fragment of the amino acid sequence set forth in SEQ ID NO: 295. In some embodiments, proteins comprising VP64-SV40-P65-RTA or fragments of VP64-SV40-P65-RTA or homologs of VP64-SV40-P65-RTA or VP64-SV40-P65-RTA fragments are referred to as “VP64-SV40-P65-RTA variants.” A VP64-SV40-P65-RTA variant shares homology to VP64-SV40-P65-RTA, or a fragment thereof. For example a VP64-SV40-P65-RTA variant is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to a VP64-SV40-P65-RTA as set forth in SEQ ID NO: 295. In some embodiments, the VP64-SV40-P65-RTA variant comprises a fragment of VP64-SV40-P65-RTA, such that the fragment is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to the corresponding fragment of a VP64-SV40-P65-RTA as set forth in SEQ ID NO: 295. In some embodiments, the VP64-SV40-P65-RTA comprises the amino acid sequence set forth in SEQ ID NO: 295. In some embodiments, the VP64-SV40-P65-RTA comprises an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO: 294.

Some aspects of this disclosure provide fusion proteins comprising (i) a Cas9 protein; and (ii) a effector domain. In some aspects, the fusion proteins provided herein further include (iii) a DNA-binding protein, for example, a zinc-finger domain, a TALE, or a second Cas9 protein. Without wishing to be bound by any particular theory, fusing a DNA-binding protein (e.g., a second Cas9 protein) to a fusion protein comprising (i) a protein; and (ii) a effector domain may be useful for improving specificity of the fusion protein to a target nucleic acid sequence, or for improving specificity or binding affinity of the fusion protein to bind a target nucleic acid sequence that does not contain the canonical PAM (5′-NGG-3′) sequence. In some embodiments, the second Cas9 protein is any of the Cas9 proteins provided herein. In some embodiments, the second Cas9 protein is fused to the fusion protein N-terminal to the Cas9 protein. In some embodiments, the second Cas9 protein is fused to the fusion protein C-terminal to the Cas9 protein. In some embodiments, the Cas9 protein and the second Cas9 protein are fused via a linker.

Further provided herein are complexes comprising any of the fusion proteins provided herein, a first guide RNA bound to the Cas9 protein of the fusion protein, and a second guide RNA bound to the second Cas9 protein of the fusion protein. In some embodiments, the first guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a first target sequence and the second guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a second target sequence. In some embodiments, the first guide RNA and/or the second guide RNA is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides long. In some embodiments, the first guide RNA and the second guide RNA are different. In some embodiments, the first guide RNA comprises a sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 contiguous nucleotides that is complementary to a first target sequence, and wherein the second guide RNA comprises a sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 contiguous nucleotides that is complementary to a second target sequence. In some embodiments, the first target sequence and the second target sequence are different. In some embodiments, the first target sequence and the second target sequence are DNA sequences. In some embodiments, the first target sequence and the second target sequence are in the genome of a mammal. In some embodiments, the first target sequence and the second target sequence are in the genome of a human. In some embodiments, the first target sequence is within 30 nucleotides of the second target sequence. In some embodiments, the 3′ end of the first target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′). In some embodiments, the 3′ end of the second target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′).

In some embodiments, the general architecture of exemplary Cas9 fusion proteins provided herein is of the structure:

[NH₂]-[effector domain]-[Cas9]-[second Cas9 protein]-[COOH];

[NH₂]-[second Cas9 protein]-[Cas9]-[effector domain]-[COOH];

[NH₂]-[Cas9]-[effector domain]-[second Cas9 protein]-[COOH];

[NH₂]-[second Cas9 protein]-[effector or domain]-[Cas9]-[COOH];

[NH₂]-[UGI]-[effector domain]-[Cas9]-[second Cas9 protein]-[COOH];

[NH₂]-[UGI]-[second Cas9 protein]-[Cas9]-[effector domain]-[COOH];

[NH₂]-[UGI]-[Cas9]-[effector domain]-[second Cas9 protein]-[COOH];

[NH₂]-[UGI]-[second Cas9 protein]-[effector domain]-[Cas9]-[COOH];

[NH₂]-[effector domain]-[Cas9]-[second Cas9 protein]-[UGI]-[COOH];

[NH₂]-[second Cas9 protein]-[Cas9]-[effector domain]-[UGI]-[COOH];

[NH₂]-[Cas9]-[effector domain]-[second Cas9 protein]-[UGI]-[COOH]; or

[NH₂]-[second Cas9 protein]-[effector domain]-[Cas9]-[UGI]-[COOH];

wherein NH₂ is the N-terminus of the fusion protein, and COOH is the C-terminus of the fusion protein. In some embodiments, the “]-[” used in the general architecture above indicates the presence of an optional linker sequence. In other examples, the general architecture of exemplary Cas9 fusion proteins provided herein is of the structure:

[NH₂]-[effector domain]-[Cas9]-[second Cas9 protein]-[COOH];

[NH₂]-[second Cas9 protein]-[Cas9]-[effector domain]-[COOH];

[NH₂]-[Cas9]-[effector domain]-[second Cas9 protein]-[COOH];

[NH₂]-[second Cas9 protein]-[effector domain]-[Cas9]-[COOH];

[NH₂]-[UGI]-[effector domain]-[Cas9]-[second Cas9 protein]-[COOH],

[NH₂]-[UGI]-[second Cas9 protein]-[Cas9]-[effector domain]-[COOH];

[NH₂]-[UGI]-[Cas9]-[effector domain]-[second Cas9 protein]-[COOH];

[NH₂]-[UGI]-[second Cas9 protein]-[effector domain]-[Cas9]-[COOH];

[NH₂]-[effector domain]-[Cas9]-[second Cas9 protein]-[UGI]-[COOH];

[NH₂]-[second Cas9 protein]-[Cas9]-[effector domain]-[UGI]-[COOH];

[NH₂]-[Cas9]-[effector domain]-[second Cas9 protein]-[UGI]-[COOH]; or

[NH₂]-[second Cas9 protein]-[effector domain]-[Cas9]-[UGI]-[COOH];

wherein NH₂ is the N-terminus of the fusion protein, and COOH is the C-terminus of the fusion protein. In some embodiments, the “-” used in the general architecture above indicates the presence of an optional linker sequence. In some embodiments, the second Cas9 is a dCas9 protein. In some examples, the general architecture of exemplary Cas9 fusion proteins provided herein comprises a structure as shown in FIG. 8. It should be appreciated that any of the proteins provided in any of the general architectures of exemplary Cas9 fusion proteins may be connected by one or more of the linkers provided herein. In some embodiments, the linkers are the same. In some embodiments, the linkers are different. In some embodiments, one or more of the proteins provided in any of the general architectures of exemplary Cas9 fusion proteins are not fused via a linker. In some embodiments, the fusion proteins further comprise a nuclear targeting sequence, for example a nuclear localization sequence. In some embodiments, fusion proteins provided herein further comprise a nuclear localization sequence (NLS). In some embodiments, the NLS is fused to the N-terminus of the fusion protein. In some embodiments, the NLS is fused to the C-terminus of the fusion protein. In some embodiments, the NLS is fused to the N-terminus of the second Cas9 protein. In some embodiments, the NLS is fused to the C-terminus of the second Cas9 protein. In some embodiments, the NLS is fused to the N-terminus of the Cas9 protein. In some embodiments, the NLS is fused to the C-terminus of the Cas9 protein. In some embodiments, the NLS is fused to the N-terminus of the effector domain. In some embodiments, the NLS is fused to the C-terminus of the effector domain. In some embodiments, the NLS is fused to the N-terminus of the UGI protein. In some embodiments, the NLS is fused to the C-terminus of the UGI protein. In some embodiments, the NLS is fused to the fusion protein via one or more linkers. In some embodiments, the NLS is fused to the fusioin protein without a linker.

Uracil Glycosylase Inhibitor Fusion Proteins

Some aspects of this disclosure provide fusion proteins comprising a Cas9 protein fused to a effector domain, e.g., a deaminase, and a uracil glycosylase inhibitor (UGI). In some embodiments, the fusion protein comprises the structure:

[deaminase]-[optional linker sequence]-[Cas9]-[optional linker sequence]-[UGI];

[deaminase]-[optional linker sequence]-[UGI]-[optional linker sequence]-[Cas9];

[UGI]-[optional linker sequence]-[deaminase]-[optional linker sequence]-[Cas9];

[UGI]-[optional linker sequence]-[Cas9]-[optional linker sequence]-[deaminase];

[Cas9]-[optional linker sequence]-[deaminase]-[optional linker sequence]-[UGI]; or

[Cas9]-[optional linker sequence]-[UGI]-[optional linker sequence]-[deaminase].

In some embodiments, the fusion proteins do not comprise a linker sequence. In some embodiments, one or both of the optional linker sequences are present.

In some embodiments, the fusion protein further comprises a second Cas9 protein. For example, the second Cas9 protein may be any of the Cas9 proteins provided herein. In some embodiments, fusion protein comprises the structure:

[deaminase]-[Cas9]-[UGI]; [deaminase]-[UGI]-[Cas9];

[UGI]-[deaminase]-[Cas9];

[UGI]-[Cas9]-[deaminase];

[Cas9]-[deaminase]-[UGI];

[Cas9]-[UGI]-[deaminase];

[second Cas9]-[deaminase]-[Cas9]-[UGI];

[second Cas9]-[deaminase]-[UGI]-[Cas9];

[second Cas9]-[UGI]-[deaminase]-[Cas9];

[second Cas9]-[UGI]-[Cas9]-[deaminase];

[second Cas9]-[Cas9]-[deaminase]-[UGI];

[second Cas9]-[Cas9]-[UGI]-[deaminase];

[deaminase]-[second Cas9]-[Cas9]-[UGI];

[deaminase]-[second Cas9]-[UGI]-[Cas9];

[UGI]-[second Cas9]-[deaminase]-[Cas9];

[UGI]-[second Cas9]-[Cas9]-[deaminase];

[Cas9]-[second Cas9]-[deaminase]-[UGI];

[Cas9]-[second Cas9]-[UGI]-[deaminase]

[deaminase]-[Cas9]-[second Cas9]-[UGI];

[deaminase]-[UGI]-[second Cas9]-[Cas9];

[UGI]-[deaminase]-[second Cas9]-[Cas9];

[UGI]-[Cas9]-[second Cas9]-[deaminase];

[Cas9]-[deaminase]-[second Cas9]-[UGI];

[Cas9]-[UGI]-[second Cas9]-[deaminase];

[deaminase]-[Cas9]-[UGI]-[second Cas9];

[deaminase]-[UGI]-[Cas9]-[second Cas9];

[UGI]-[deaminase]-[Cas9]-[second Cas9];

[UGI]-[Cas9]-[deaminase]-[second Cas9];

[Cas9]-[deaminase]-[UGI]-[second Cas9]; or

[Cas9]-[UGI]-[deaminase]-[second Cas9].

In some embodiments, the “-” used in the general architecture above indicates the presence of an optional linker sequence. In some embodiments, the fusion proteins comprising a UGI further comprise a nuclear targeting sequence, for example a nuclear localization sequence. In some embodiments, fusion proteins provided herein further comprise a nuclear localization sequence (NLS). In some embodiments, the NLS is fused to the N-terminus of the fusion protein. In some embodiments, the NLS is fused to the C-terminus of the fusion protein. In some embodiments, the NLS is fused to the N-terminus of the UGI protein. In some embodiments, the NLS is fused to the C-terminus of the UGI protein. In some embodiments, the NLS is fused to the N-terminus of the Cas9 protein. In some embodiments, the NLS is fused to the C-terminus of the Cas9 protein. In some embodiments, the NLS is fused to the N-terminus of the deaminase. In some embodiments, the NLS is fused to the C-terminus of the deaminase. In some embodiments, the NLS is fused to the N-terminus of the second Cas9. In some embodiments, the NLS is fused to the C-terminus of the second Cas9. In some embodiments, the NLS is fused to the fusion protein via one or more linkers. In some embodiments, the NLS is fused to the fusion protein without a linker.

In some embodiments, the UGI comprises a wild type UGI or a UGI as set forth in SEQ ID NO: 553. In some embodiments, the UGI proteins provided herein include fragments of UGI and proteins homologous to a UGI or a UGI fragment. For example, in some embodiments, a UGI comprises a fragment of the amino acid sequence set forth in SEQ ID NO: 553. In some embodiments, a UGI comprises an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 553 or an amino acid sequence homologous to a fragment of the amino acid sequence set forth in SEQ ID NO: 553. In some embodiments, proteins comprising UGI or fragments of UGI or homologs of UGI or UGI fragments are referred to as “UGI variants.” A UGI variant shares homology to UGI, or a fragment thereof. For example a UGI variant is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to a wild type UGI or a UGI as set forth in SEQ ID NO: 553. In some embodiments, the UGI variant comprises a fragment of UGI, such that the fragment is at least about 70% identical, at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% to the corresponding fragment of wild type UGI or a UGI as set forth in SEQ ID NO: 553. In some embodiments, the UGI comprises the following amino acid sequence: >sp|P14739|UNGI_BPPB2 Uracil-DNA glycosylase inhibitor MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLT SDAPEYKPWALVIQDSNGENKIKML (SEQ ID NO: 553)

Suitable UGI protein and nucleotide sequences are provided herein and additional suitable UGI sequences are known to those in the art, and include, for example, those published in Wang et al., Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase. J Biol. Chem. 264:1163-1171(1989); Lundquist et al., Site-directed mutagenesis and characterization of uracil-DNA glycosylase inhibitor protein. Role of specific carboxylic amino acids in complex formation with Escherichia coli uracil-DNA glycosylase. J Biol. Chem. 272:21408-21419(1997); Ravishankar et al., X-ray analysis of a complex of Escherichia coli uracil DNA glycosylase (EcUDG) with a proteinaceous inhibitor. The structure elucidation of a prokaryotic UDG. Nucleic Acids Res. 26:4880-4887(1998); and Putnam et al., Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase. J Mol. Biol. 287:331-346(1999), the entire contents of each are incorporated herein by reference.

It should be appreciated that additional proteins may be uracil glycosylase inhibitors. For example, other proteins that are capable of inhibiting (e.g., sterically blocking) a uracil-DNA glycosylase base-excision repair enzyme are within the scope of this disclosure. In some embodiments, a uracil glycosylase inhibitor is a protein that binds DNA. In some embodiments, a uracil glycosylase inhibitor is a protein that binds single-stranded DNA. For example, a uracil glycosylase inhibitor may be a Erwinia tasmaniensis single-stranded binding protein. In some embodiments, the single-stranded binding protein comprises the amino acid sequence (SEQ ID NO: 303). In some embodiments, a uracil glycosylase inhibitor is a protein that binds uracil. In some embodiments, a uracil glycosylase inhibitor is a protein that binds uracil in DNA. In some embodiments, a uracil glycosylase inhibitor is a catalytically inactive uracil DNA-glycosylase protein. In some embodiments, a uracil glycosylase inhibitor is a catalytically inactive uracil DNA-glycosylase protein that does not excise uracil from the DNA. For example, a uracil glycosylase inhibitor is a UdgX. In some embodiments, the UdgX comprises the amino acid sequence (SEQ ID NO: 304). As another example, a uracil glycosylase inhibitor is a catalytically inactive UDG. In some embodiments, a catalytically inactive UDG comprises the amino acid sequence (SEQ ID NO: 305). It should be appreciated that other uracil glycosylase inhibitors would be apparent to the skilled artisan and are within the scope of this disclosure.

Erwinia tasmaniensis SSB (themostable single- stranded DNA binding protein) (SEQ ID NO: 303) MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGETK EKTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGALQTRKWTDQAGVEKYTT EVVVNVGGTMQMLGGRSQGGGASAGGQNGGSNNGWGQPQQPQGGNQFSGG AQQQARPQQQPQQNNAPANNEPPIDFDDDIP UdgX (binds to Uracil in DNA but does not excise) (SEQ ID NO: 304) MAGAQDFVPHTADLAELAAAAGECRGCGLYRDATQAVFGAGGRSARIMMI GEQPGDKEDLAGLPFVGPAGRLLDRALEAADIDRDALYVTNAVKHFKFTR AAGGKRRIHKTPSRTEVVACRPWLIAEMTSVEPDVVVLLGATAAKALLGN DFRVTQHRGEVLHVDDVPGDPALVATVHPSSLLRGPKEERESAFAGLVDD LRVAADVRP UDG (catalytically inactive human UDG, binds to Uracil in DNA but does not excise) (SEQ ID NO: 305) MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKK APAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKK HLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVI LGQEPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGD LSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLV FLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELL QKSGKKPIDWKEL

High Fidelity Cas9

Some aspects of the disclosure provide high fidelity Cas9 proteins. In some embodiments, high fidelity Cas9 proteins have decreased electrostatic interactions between the Cas9 protein and a sugar-phosphate backbone of a DNA, as compared to a wild-type Cas9 domain. In some embodiments, any of the Cas9 proteins provided herein comprise one or more mutations that decrease the association between the Cas9 protein and a sugar-phosphate backbone of a DNA. In some embodiments, any of the Cas9 proteins provided herein comprise one or more mutations that decrease the association between the Cas9 protein and a sugar-phosphate backbone of a DNA by at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or at least 95%. In some embodiments, any of the Cas9 proteins provided herein comprise one or more of a N497X, a R661X, a Q695X, and/or a Q926X mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid. In some embodiments, any of the Cas9 proteins provided herein comprise one or more of a N497A, a R661A, a Q695A, and/or a Q926A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises a D10A mutation of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the Cas9 protein comprises the amino acid sequence as set forth in SEQ ID NO: 306. High fidelity Cas9 proteins have been described in the art and would be apparent to the skilled artisan. For example, high fidelity Cas9 proteins have been described in Kleinstiver, B. P., et al. “High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.” Nature 529, 490-495 (2016); and Slaymaker, I. M., et al. “Rationally engineered Cas9 nucleases with improved specificity.” Science 351, 84-88 (2015); the entire contents of each are incorporated herein by reference. It should be appreciated that, based on the present disclosure and knowledge in the art, that mutations in any Cas9 protein may be generated to make high fidelity Cas9 proteins that have decreased electrostatic interactions between the Cas9 protein and a sugar-phosphate backbone of a DNA, as compared to a wild-type Cas9 domain.

Cas9 domain where mutations relative to Cas9 of SEQ ID NO: 9 are shown in bold and underlines.

(SEQ ID NO: 306) MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGA LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTP NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT A FDK NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ LKRRRYTGWG A LSRKLINGIRDKQSGKTILDFLKSDGFANRNFM A LIHDD SLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV MGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP VENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDD SIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL TKAERGGLSELDKAGFIKRQLVETR A ITKHVAQILDSRMNTKYDENDKLI REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK YPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI TLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEV QTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ SITGLYETRIDLSQLGGD Cas9 Proteins with Reduced PAM Exclusivity

Some aspects of the disclosure provide Cas9 proteins that have different PAM specificities. Typically, Cas9 proteins, such as Cas9 from S. pyogenes (spCas9), require a canonical NGG PAM sequence to bind a particular nucleic acid region. This may limit the ability to of the Cas9 protein to bind to a particular nucleotide sequence within a genome. Accordingly, in some embodiments, any of the Cas proteins provided herein may be capable of binding a nucleotide sequence that does not contain a canonical (e.g., NGG) PAM sequence. For example, Cas9 proteins that bind non-canonical PAM sequences have been described in Kleinstiver, B. P., et al., “Engineered CRISPR-Cas9 nucleases with altered PAM specificities” Nature 523, 481-485 (2015); and Kleinstiver, B. P., et al., “Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition” Nature Biotechnology 33, 1293-1298 (2015); the entire contents of each are hereby incorporated by reference.

In some embodiments, the Cas9 protein is a Cas9 protein from Staphylococcus aureus (SaCas9). In some embodiments, the SaCas9 protein is a nuclease active SaCas9, a nuclease inactive SaCas9 (SaCas9d), or a SaCas9 nickase (SaCas9n). In some embodiments, the SaCas9 comprises the amino acid sequence SEQ ID NO: 307. In some embodiments, the SaCas9 comprises a N579X mutation of SEQ ID NO: 307, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 9-262, wherein X is any amino acid except for N. In some embodiments, the SaCas9 comprises a N579A mutation of SEQ ID NO: 307, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SaCas9 protein, the SaCas9d protein, or the SaCas9n protein can bind to a nucleic acid sequence having a non-canonical PAM. In some embodiments, the SaCas9 protein, the SaCas9d protein, or the SaCas9n protein can bind to a nucleic acid sequence having a NNGRRT PAM sequence. In some embodiments, the SaCas9 protein comprises one or more of a E781X, N967X, or R1014X mutation of SEQ ID NO: 307, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid. In some embodiments, the SaCas9 protein comprises one or more of a E781K, N967K, or R1014H mutation of SEQ ID NO: 307, or one or more corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SaCas9 protein comprises a E781K, a N967K, and a R1014H mutation of SEQ ID NO: 307, or corresponding mutations in any of the amino acid sequences provided in SEQ ID NOs: 10-262. It should be appreciated that these mutations may be combined with any of the other mutations provided herein

In some embodiments, the Cas9 protein of any of the fusion proteins provided herein comprises an amino acid sequence that is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to any one of SEQ ID NOs: 307-309. In some embodiments, the Cas9 protein of any of the fusion proteins provided herein comprises the amino acid sequence of any one of SEQ ID NOs: 307-309. In some embodiments, the Cas9 protein of any of the fusion proteins provided herein consists of the amino acid sequence of any one of SEQ ID NOs: 307-309.

Exemplary SaCas9 sequence (SEQ ID NO: 307) KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKR GARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLS EEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVA ELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTY IDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAY NADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAK EILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQI AKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAIN LILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVK RSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQT NERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPF NYEVDHIIPRSVSFDNSFNNKVLVKQEE N SKKGNRTPFQYLSSSDSKISY ETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFINRNLVDTRY ATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYK EIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLI VNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEK NPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSR NKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAK KLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNRIEVNMIDITY REYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIK KG

Residue N579 of SEQ ID NO: 307, which is underlined and in bold, may be mutated (e.g., to a A579) to yield a SaCas9 nickase.

Exemplary SaCas9n sequence (SEQ ID NO: 308) KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKR GARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLS EEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVA ELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTY IDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAY NADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAK EILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQI AKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAIN LILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVK RSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQT NERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPF NYEVDHIIPRSVSFDNSFNNKVLVKQEE A SKKGNRTPFQYLSSSDSKISY ETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFINRNLVDTRY ATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYK EIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLI VNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEK NPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSR NKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAK KLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNRIEVNMIDITY REYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIK KG.

Residue A579 of SEQ ID NO: 308, which can be mutated from N579 of SEQ ID NO: 307 to yield a SaCas9 nickase, is underlined and in bold.

Exemplary SaKKH Cas9 (SEQ ID NO: 309) KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKR GARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLS EEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVA ELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTY IDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAY NADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAK EILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQI AKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAIN LILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVK RSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQT NERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPF NYEVDHIIPRSVSFDNSFNNKVLVKQEE A SKKGNRTPFQYLSSSDSKISY ETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFINRNLVDTRY ATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYK EIFITPHQIKHIKDFKDYKYSHRVDKKPNR K LINDTLYSTRKDDKGNTLI VNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEK NPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSR NKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAK KLKKISNQAEFIASFY K NDLIKINGELYRVIGVNNDLLNRIEVNMIDITY REYLENMNDKRPP H IIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIK KG.

Residue A579 of SEQ ID NO: 309, which can be mutated from N579 of SEQ ID NO: 307 to yield a SaCas9 nickase, is underlined and in bold. Residues K781, K967, and H1014 of SEQ ID NO: 309, which can be mutated from E781, N967, and R1014 of SEQ ID NO: 307 to yield a SaKKH Cas9 are underlined and in italics.

In some embodiments, the Cas9 protein is a Cas9 protein from Streptococcus pyogenes (SpCas9). In some embodiments, the SpCas9 protein is a nuclease active SpCas9, a nuclease inactive SpCas9 (SpCas9d), or a SpCas9 nickase (SpCas9n). In some embodiments, the SpCas9 comprises the amino acid sequence SEQ ID NO: 9. In some embodiments, the SpCas9 comprises a D10X mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid except for D. In some embodiments, the SpCas9 comprises a D10A mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein, the SpCas9d protein, or the SpCas9n protein can bind to a nucleic acid sequence having a non-canonical PAM. In some embodiments, the SpCas9 protein, the SpCas9d protein, or the SpCas9n protein can bind to a nucleic acid sequence having a NGG, a NGA, or a NGCG PAM sequence. In some embodiments, the SpCas9 protein comprises one or more of a D1135X, R1335X, and T1337X mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid. In some embodiments, the SpCas9 protein comprises one or more of a D1135E, R1335Q, and T1337R mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein comprises a D1135E, a R1335Q, and a T1335R mutation of SEQ ID NO: 9, or corresponding mutations in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein comprises one or more of a D1135X, R1335X, and T1337X mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid. In some embodiments, the SpCas9 protein comprises one or more of a D1135V, R1335Q, and T1337R mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein comprises a D1135V, a R1335Q, and a T1337R mutation of SEQ ID NO: 9, or corresponding mutations in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein comprises one or more of a D1135X, G1218X, R1335X, and T1337X mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X is any amino acid. In some embodiments, the SpCas9 protein comprises one or more of a D1135V, G1218R, R1335Q, and T1337R mutation of SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. In some embodiments, the SpCas9 protein comprises a D1135V, a G1218R, a R1335Q, and a T1337R mutation of SEQ ID NO: 9, or corresponding mutations in any of the amino acid sequences provided in SEQ ID NOs: 10-262. It should be appreciated that these mutations may be combined with any of the other mutations provided herein

In some embodiments, the Cas9 protein of any of the fusion proteins provided herein comprises an amino acid sequence that is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% identical to any one of SEQ ID NOs: 9, 310-313. In some embodiments, the Cas9 domain of any of the fusion proteins provided herein comprises the amino acid sequence of any one of SEQ ID NOs: 9, 310-313. In some embodiments, the Cas9 domain of any of the fusion proteins provided herein consists of the amino acid sequence of any one of SEQ ID NOs: 9, 310-313.

Exemplary SpCas9 (SEQ ID NO: 9) MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGA LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTP NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ LKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDD SLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV MGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP VENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDD SIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK YPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI TLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEV QTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ SITGLYETRIDLSQLGGD Exemplary SpCas9n (SEQ ID NO: 10) DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM GRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKY PKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEIT LANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQ TGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKY SLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPED NEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP IREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQS ITGLYETRIDLSQLGGD Exemplary SpEQR Cas9 (SEQ ID NO: 311) DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM GRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKEDNLT KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKY PKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEIT LANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQ TGGFSKESILPKRNSDKLIARKKDWDPKKYGGF E SPTVAYSVLVVAKVEK GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKY SLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPED NEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP IREQAENIIHLFTLTNLGAPAAFKYFDTTIDRK Q Y R STKEVLDATLIHQS ITGLYETRIDLSQLGGD

Residues E1134, Q1334, and R1336 of SEQ ID NO: 311, which can be mutated from D1134, R1334, and T1336 of SEQ ID NO: 9 to yield a SpEQR Cas9, are underlined and in bold.

Exemplary SpVQR Cas9 (SEQ ID NO: 312) DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM GRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKY PKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEIT LANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQ TGGFSKESILPKRNSDKLIARKKDWDPKKYGGF V SPTVAYSVLVVAKVEK GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKY SLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPED NEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP IREQAENIIHLFTLTNLGAPAAFKYFDTTIDRK Q Y R STKEVLDATLIHQS ITGLYETRIDLSQLGGD

Residues V1134, Q1334, and R1336 of SEQ ID NO: 312, which can be mutated from D1134, R1334, and T1336 of SEQ ID NO: 9 to yield a SpVQR Cas9, are underlined and in bold.

Exemplary SpVRER Cas9 (SEQ ID NO: 313) DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM GRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKY PKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEIT LANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQ TGGFSKESILPKRNSDKLIARKKDWDPKKYGGF V SPTVAYSVLVVAKVEK GKSKKLKSVKELLGITIMIERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK YSLFELENGRKRMLASA R ELQKGNELALPSKYVNFLYLASHYEKLKGSPE DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRK E Y R STKEVLDATLIHQ SITGLYETRIDLSQLGGD

Residues V1134, R1217, Q1334, and R1336 of SEQ ID NO: 313, which can be mutated from D1134, G1217, R1334, and T1336 of SEQ ID NO: 9 to yield a SpVRER Cas9, are underlined and in bold.

Cas9 Complexes with Guide RNAs

Some aspects of this disclosure provide complexes comprising a Cas9 protein or a Cas9 fusion protein as provided herein, and a guide RNA bound to the Cas9 protein or the Cas9 fusion protein. In some embodiments, the guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a target sequence. In some embodiments, the guide RNA is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides long. In some embodiments, the guide RNA comprises a sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 contiguous nucleotides that is complementary to a target sequence. In some embodiments, the target sequence is a DNA sequence. In some embodiments, the target sequence is a sequence in the genome of a mammal. In some embodiments, the target sequence is a sequence in the genome of a human. In some embodiments, the 3′ end of the target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′).

Some aspects of the disclosure provide complexes comprising a first guide RNA bound to a Cas9 protein of a fusion protein, and a second guide RNA bound to a second Cas9 protein of the fusion protein. In some embodiments, the first guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a first target sequence and the second guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a second target sequence. In some embodiments, the first guide RNA and/or the second guide RNA is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides long. In some embodiments, the first guide RNA and the second guide RNA are different. In some embodiments, the first guide RNA comprises a sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 contiguous nucleotides that is complementary to a first target sequence and wherein the second guide RNA comprises a sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 contiguous nucleotides that is complementary to a second target sequence.

In some embodiments, first target sequence and the second target sequence are different. In some embodiments, the first target sequence and the second target sequence are DNA sequences. In some embodiments, the first target sequence and the second target sequence are in the genome of a mammal. In some embodiments, the first target sequence and the second target sequence are in the genome of a human. In some embodiments, the first target sequence is within at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150, or 200 nucleotides of the second target sequence. In some embodiments, the 3′-end of the first target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′). In some embodiments, the 3′-end of the second target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′).

Methods of Using Cas9 Fusion Proteins

Some aspects of this disclosure provide methods of using the Cas9 proteins, fusion proteins, or complexes provided herein. For example, some aspects of this disclosure provide methods comprising contacting a DNA molecule (a) with any of the the Cas9 proteins or fusion proteins provided herein, and with at least one guide RNA, wherein the guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a target sequence; or (b) with a Cas9 protein, a Cas9 fusion protein, or a Cas9 protein or fusion protein complex with at least one gRNA as provided herein. In some embodiments, the 3′ end of the target sequence is not immediately adjacent to the canonical PAM sequence (5′-NGG-3′). In some embodiments, the 3′ end of the target sequence is immediately adjacent to an AGC, GAG, TTT, GTG, or CAA sequence.

In some embodiments, the target DNA sequence comprises a sequence associated with a disease or disorder. In some embodiments, the target DNA sequence comprises a point mutation associated with a disease or disorder. In some embodiments, the activity of the Cas9 protein, the Cas9 fusion protein, or the complex results in a correction of the point mutation. In some embodiments, the target DNA sequence comprises a T-C point mutation associated with a disease or disorder, and wherein the deamination of the mutant C base results in a sequence that is not associated with a disease or disorder. In some embodiments, the target DNA sequence encodes a protein and wherein the point mutation is in a codon and results in a change in the amino acid encoded by the mutant codon as compared to the wild-type codon. In some embodiments, the deamination of the mutant C results in a change of the amino acid encoded by the mutant codon. In some embodiments, the deamination of the mutant C results in the codon encoding the wild-type amino acid. In some embodiments, the contacting is in vivo in a subject. In some embodiments, the subject has or has been diagnosed with a disease or disorder. In some embodiments, the disease or disorder is cystic fibrosis, phenylketonuria, epidermolytic hyperkeratosis (EHK), Charcot-Marie-Toot disease type 4J, neuroblastoma (NB), von Willebrand disease (vWD), myotonia congenital, hereditary renal amyloidosis, dilated cardiomyopathy (DCM), hereditary lymphedema, familial Alzheimer's disease, HIV, Prion disease, chronic infantile neurologic cutaneous articular syndrome (CINCA), desmin-related myopathy (DRM), a neoplastic disease associated with a mutant PI3KCA protein, a mutant CTNNB 1 protein, a mutant HRAS protein, or a mutant p53 protein.

Some embodiments provide methods for using the Cas9 DNA editing fusion proteins provided herein. In some embodiments, the fusion protein is used to introduce a point mutation into a nucleic acid by deaminating a target nucleobase, e.g., a C residue. In some embodiments, the deamination of the target nucleobase results in the correction of a genetic defect, e.g., in the correction of a point mutation that leads to a loss of function in a gene product. In some embodiments, the genetic defect is associated with a disease or disorder, e.g., a lysosomal storage disorder or a metabolic disease, such as, for example, type I diabetes. In some embodiments, the methods provided herein are used to introduce a deactivating point mutation into a gene or allele that encodes a gene product that is associated with a disease or disorder. For example, in some embodiments, methods are provided herein that employ a Cas9 DNA editing fusion protein to introduce a deactivating point mutation into an oncogene (e.g., in the treatment of a proliferative disease). A deactivating mutation may, in some embodiments, generate a premature stop codon in a coding sequence, which results in the expression of a truncated gene product, e.g., a truncated protein lacking the function of the full-length protein.

In some embodiments, the purpose of the methods provide herein is to restore the function of a dysfunctional gene via genome editing. The Cas9 deaminase fusion proteins provided herein can be validated for gene editing-based human therapeutics in vitro, e.g., by correcting a disease-associated mutation in human cell culture. It will be understood by the skilled artisan that the fusion proteins provided herein, e.g., the fusion proteins comprising a Cas9 domain and a nucleic acid deaminase domain can be used to correct any single point T->C or A->G mutation. In the first case, deamination of the mutant C back to U corrects the mutation, and in the latter case, deamination of the C that is base-paired with the mutant G, followed by a round of replication, corrects the mutation.

An exemplary disease-relevant mutation that can be corrected by the provided fusion proteins in vitro or in vivo is the H1047R (A3140G) polymorphism in the PI3KCA protein. The phosphoinositide-3-kinase, catalytic alpha subunit (PI3KCA) protein acts to phosphorylate the 3-OH group of the inositol ring of phosphatidylinositol. The PI3KCA gene has been found to be mutated in many different carcinomas, and thus it is considered to be a potent oncogene.⁵⁰ In fact, the A3140G mutation is present in several NCI-60 cancer cell lines, such as, for example, the HCT116, SKOV3, and T47D cell lines, which are readily available from the American Type Culture Collection (ATCC).⁵¹

In some embodiments, a cell carrying a mutation to be corrected, e.g., a cell carrying a point mutation, e.g., an A3140G point mutation in exon 20 of the PI3KCA gene, resulting in a H1047R substitution in the PI3KCA protein, is contacted with an expression construct encoding a Cas9 deaminase fusion protein and an appropriately designed sgRNA targeting the fusion protein to the respective mutation site in the encoding PI3KCA gene. Control experiments can be performed where the sgRNAs are designed to target the fusion enzymes to non-C residues that are within the PI3KCA gene. Genomic DNA of the treated cells can be extracted, and the relevant sequence of the PI3KCA genes PCR amplified and sequenced to assess the activities of the fusion proteins in human cell culture.

It will be understood that the example of correcting point mutations in PI3KCA is provided for illustration purposes and is not meant to limit the instant disclosure. The skilled artisan will understand that the instantly disclosed DNA-editing fusion proteins can be used to correct other point mutations and mutations associated with other cancers and with diseases other than cancer including other proliferative diseases.

The successful correction of point mutations in disease-associated genes and alleles opens up new strategies for gene correction with applications in therapeutics and basic research. Site-specific single-base modification systems like the disclosed fusions of Cas9 and deaminase enzymes or domains also have applications in “reverse” gene therapy, where certain gene functions are purposely suppressed or abolished. In these cases, site-specifically mutating Trp (TGG), Gln (CAA and CAG), or Arg (CGA) residues to premature stop codons (TAA, TAG, TGA) can be used to abolish protein function in vitro, ex vivo, or in vivo.

The instant disclosure provides methods for the treatment of a subject diagnosed with a disease associated with or caused by a point mutation that can be corrected by a Cas9 DNA editing fusion protein provided herein. For example, in some embodiments, a method is provided that comprises administering to a subject having such a disease, e.g., a cancer associated with a PI3KCA point mutation as described above, an effective amount of a Cas9 deaminase fusion protein that corrects the point mutation or introduces a deactivating mutation into the disease-associated gene. In some embodiments, the disease is a proliferative disease. In some embodiments, the disease is a genetic disease. In some embodiments, the disease is a neoplastic disease. In some embodiments, the disease is a metabolic disease. In some embodiments, the disease is a lysosomal storage disease. Other diseases that can be treated by correcting a point mutation or introducing a deactivating mutation into a disease-associated gene will be known to those of skill in the art, and the disclosure is not limited in this respect.

The instant disclosure provides methods for the treatment of additional diseases or disorders, e.g., diseases or disorders that are associated or caused by a point mutation that can be corrected by deaminase-mediated gene editing. Some such diseases are described herein, and additional suitable diseases that can be treated with the strategies and fusion proteins provided herein will be apparent to those of skill in the art based on the instant disclosure. Exemplary suitable diseases and disorders are listed below. It will be understood that the numbering of the specific positions or residues in the respective sequences depends on the particular protein and numbering scheme used. Numbering might be different, e.g., in precursors of a mature protein and the mature protein itself, and differences in sequences from species to species may affect numbering. One of skill in the art will be able to identify the respective residue in any homologous protein and in the respective encoding nucleic acid by methods well known in the art, e.g., by sequence alignment and determination of homologous residues. Exemplary suitable diseases and disorders include, without limitation, cystic fibrosis (see, e.g., Schwank et al., Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cellstem cell. 2013; 13: 653-658; and Wu et. al., Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cellstem cell. 2013; 13: 659-662, neither of which uses a deaminase fusion protein to correct the genetic defect); phenylketonuria—e.g., phenylalanine to serine mutation at position 835 (mouse) or 240 (human) or a homologous residue in phenylalanine hydroxylase gene (T>C mutation)—see, e.g., McDonald et al., Genomics. 1997; 39:402-405; Bernard-Soulier syndrome (BSS)—e.g., phenylalanine to serine mutation at position 55 or a homologous residue, or cysteine to arginine at residue 24 or a homologous residue in the platelet membrane glycoprotein IX (T>C mutation)—see, e.g., Noris et al., British Journal of Haematology. 1997; 97: 312-320, and Ali et al., Hematol. 2014; 93: 381-384; epidermolytic hyperkeratosis (EHK)—e.g., leucine to proline mutation at position 160 or 161 (if counting the initiator methionine) or a homologous residue in keratin 1 (T>C mutation)—see, e.g., Chipev et al., Cell. 1992; 70: 821-828, see also accession number P04264 in the UNIPROT database at www[dot]uniprot[dot]org; chronic obstructive pulmonary disease (COPD)—e.g., leucine to proline mutation at position 54 or 55 (if counting the initiator methionine) or a homologous residue in the processed form of α₁-antitrypsin or residue 78 in the unprocessed form or a homologous residue (T>C mutation)—see, e.g., Poller et al., Genomics. 1993; 17: 740-743, see also accession number P01011 in the UNIPROT database; Charcot-Marie-Toot disease type 4J—e.g., isoleucine to threonine mutation at position 41 or a homologous residue in FIG. 4 (T>C mutation)—see, e.g., Lenk et al., PLoS Genetics. 2011; 7: e1002104; neuroblastoma (NB)—e.g., leucine to proline mutation at position 197 or a homologous residue in Caspase-9 (T>C mutation)—see, e.g., Kundu et al., 3 Biotech. 2013, 3:225-234; von Willebrand disease (vWD)—e.g., cysteine to arginine mutation at position 509 or a homologous residue in the processed form of von Willebrand factor, or at position 1272 or a homologous residue in the unprocessed form of von Willebrand factor (T>C mutation)—see, e.g., Lavergne et al., Br. J. Haematol. 1992, see also accession number P04275 in the UNIPROT database; 82: 66-72; myotonia congenital—e.g., cysteine to arginine mutation at position 277 or a homologous residue in the muscle chloride channel gene CLCN1 (T>C mutation)—see, e.g., Weinberger et al., The J. of Physiology. 2012; 590: 3449-3464; hereditary renal amyloidosis—e.g., stop codon to arginine mutation at position 78 or a homologous residue in the processed form of apolipoprotein AII or at position 101 or a homologous residue in the unprocessed form (T>C mutation)—see, e.g., Yazaki et al., Kidney Int. 2003; 64: 11-16; dilated cardiomyopathy (DCM)—e.g., tryptophan to Arginine mutation at position 148 or a homologous residue in the FOXD4 gene (T>C mutation), see, e.g., Minoretti et. al., Int. J. of Mol. Med. 2007; 19: 369-372; hereditary lymphedema—e.g., histidine to arginine mutation at position 1035 or a homologous residue in VEGFR3 tyrosine kinase (A>G mutation), see, e.g., Irrthum et al., Am. J. Hum. Genet. 2000; 67: 295-301; familial Alzheimer's disease—e.g., isoleucine to valine mutation at position 143 or a homologous residue in presenilin1 (A>G mutation), see, e.g., Gallo et. al., J Alzheimer's disease. 2011; 25: 425-431; Prion disease—e.g., methionine to valine mutation at position 129 or a homologous residue in prion protein (A>G mutation)—see, e.g., Lewis et. al., J. of General Virology. 2006; 87: 2443-2449; chronic infantile neurologic cutaneous articular syndrome (CINCA)—e.g., Tyrosine to Cysteine mutation at position 570 or a homologous residue in cryopyrin (A>G mutation)—see, e.g., Fujisawa et. al. Blood. 2007; 109: 2903-2911; and desmin-related myopathy (DRM)—e.g., arginine to glycine mutation at position 120 or a homologous residue in cB crystallin (A>G mutation)—see, e.g., Kumar et al., J. Biol. Chem. 1999; 274: 24137-24141. The entire contents of all references and database entries is incorporated herein by reference.

The instant disclosure provides lists of genes comprising pathogenic T>C or A>G mutations, which may be corrected using any of the Cas9 fusion proteins provided herein. Provided herein, are the names of these genes, their respective SEQ ID NOs, their gene IDs, and sequences flanking the mutation site. See Tables 4 and 5. Without wishing to be bound by any particular theory, the mutations provided in Tables 4 and 5 may be corrected using the Cas9 fusions provided herein, which are able to bind to target sequences lacking the canonical PAM sequence. In some embodiments, a Cas9-deaminase fusion protein demonstrates activity on non-canonical PAMs and therefore can correct all the pathogenic T>C or A>G mutations listed in Tables 4 and 5 (SEQ ID NOs: 674-2539 and 3144-5083), respectively. In some embodiments, a Cas9-deaminase fusion protein recognizes canonical PAMs and therefore can correct the pathogenic T>C or A>G mutations with canonical PAMs, e.g., 5′-NGG-3′. It should be appreciated that a skilled artisan would understand how to design an RNA (e.g., a gRNA) to target any of the Cas9 proteins or fusion proteins provided herein to any target sequence in order to correct any of the mutations provided herein, for example, the mutations provided in Tables 4 and 5. It will be apparent to those of skill in the art that in order to target a Cas9:effector domain fusion protein as disclosed herein to a target site, e.g., a site comprising a point mutation to be edited, it is typically necessary to co-express the Cas9:effector domain fusion protein together with a guide RNA, e.g., an sgRNA. As explained in more detail elsewhere herein, a guide RNA typically comprises a tracrRNA framework allowing for Cas9 binding, and a guide sequence, which confers sequence specificity to the Cas9:effector domain fusion protein. In some embodiments, the guide RNA comprises a structure 5′-[guide sequence]-guuuuagagcuagaaauagcaaguuaaaauaaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuu uuu-3′ (SEQ ID NO: 285),

wherein the guide sequence comprises a sequence that is complementary to the target sequence. The guide sequence is typically 20 nucleotides long. The sequences of suitable guide RNAs for targeting Cas9:effector domain fusion proteins to specific genomic target sites will be apparent to those of skill in the art based on the instant disclosure. Such suitable guide RNA sequences typically comprise guide sequences that are complementary to a nucleic sequence within 50 nucleotides upstream or downstream of the target nucleotide to be edited.

Kits, Vectors, Cells

Some aspects of this disclosure provide kits comprising a nucleic acid construct, comprising (a) a nucleotide sequence encoding a Cas9 protein or a Cas9 fusion protein as provided herein; and (b) a heterologous promoter that drives expression of the sequence of (a). In some embodiments, the kit further comprises an expression construct encoding a guide RNA backbone, wherein the construct comprises a cloning site positioned to allow the cloning of a nucleic acid sequence identical or complementary to a target sequence into the guide RNA backbone.

Some aspects of this disclosure provide polynucleotides encoding a Cas9 protein of a fusion protein as provided herein. Some aspects of this disclosure provide vectors comprising such polynucleotides. In some embodiments, the vector comprises a heterologous promoter driving expression of polynucleotide.

Some aspects of this disclosure provide cells comprising a Cas9 protein, a fusion protein, a nucleic acid molecule, and/or a vector as provided herein.

The description of exemplary embodiments of the reporter systems herein is provided for illustration purposes only and not meant to be limiting. Additional reporter systems, e.g., variations of the exemplary systems described in detail above, are also embraced by this disclosure.

EXAMPLES Example 1: PACE Evolution of a Cas9 without PAM Sequence Restriction

Building PAM Libraries.

Four different protospacer target sequences were synthesized: Doench 1-5′-AAGAGAGACAGTACATGCCC-3′(SEQ ID NO: 286);

Doench 2-5′-GGAGCCCACCGAGTACCTGG-3′(SEQ ID NO: 287); G7′-5′-AGTCTCCTCAGCAAAACGAA-3′(SEQ ID NO: 288); and VEGF Target 2-5′-GACCCCCTCCACCCCGCCTC-3′ (SEQ ID NO: 289). For each protospacer target sequence, a 3′-NNN PAM library was built. While the canonical PAM sequence is 5′-NGG-3′, (e.g., an exemplary [Doench 1]-[canonical PAM] target sequence could be 5′-[AAGAGAGACAGTACATGCCC]-[NGG]-3′ (SEQ ID NO: 291)), the 3 NNN PAM libraries for each protospacer target sequence contained a fully random PAM sequence, e.g., for Doenchl 5′-AAGAGAGACAGTACATGCCCNNN-3′ (SEQ ID NO: 290), wherein N represents any nucleotide. The NNN PAM libraries thus included every possible combination of PAM sequences at the 3′ end of the respective protospacer target sequence.

Testing the Activity of Cas9 on PAM Libraries in a Co-dCas9 Luciferase Assay.

Cas9 activity was tested using a bacterial luciferase activation assay in which a fusion protein of the co subunit of Escherichia coli RNA polymerase (rpoZ) to dCas9 (see, e.g., Bikard et al., Nucleic Acids Res. 2013 August; 41(15): 7429-7437) drives the production of luciferase encoded by a nucleic acid under the control of a weak promoter comprising a sequence targeted by an sgRNA. Each PAM library was cloned into a plasmid comprising such a weak promoter, where the [target sequence]-[PAM library] nucleic acid sequences served as the sequence targeted by an sgRNA. A PAM library was cloned into the promoter for The ω-dCas9 assay was run on all four protospacer targets for both the canonical PAM and the random PAM library. FIG. 1 shows the activity of wild-type S. pyogenes Cas9 on PAM Libraries.

Evolution of Cas9 on PAM Libraries.

S. pyogenes dCas9 was fused to the co unit of RNA polymerase. The resulting ω-dCas9 fusion protein was cloned into an M13 phage-based selection phagemid (SP), comprising the entire M13 phage genome except for a functional version of the gene encoding pIII, a gene essential for the generation of infectious phage particles. The phage gene encoding pIII was provided on a separate plasmid (accessory plasmid, AP), under the control of a promoter that is transcriptionally activated by ω-dCas9. The PAM library was cloned into the promoter region of the accessory plasmid. Host cells used for the directed evolution of Cas9 proteins without PAM restriction were provided that harbored the accessory plasmid. Upon infection with a selection phage, the amount of infectious phage particles produced by a given host cell thus depends on the activity of the ω-dCas9 fusion protein encoded by the selection phage on the promoter of the accessory plasmid, which is required for the production of pIII protein. The accessory plasmid thus confers a selective advantage to those selection phages encoding ω-dCas9 fusion protein variants with an increased activity on different non-canonical PAM sequences.

A lagoon was provided and a flow of host cells comprising the accessory plasmid was generated through the lagoon. The host cells were contacted with the selection phagemid, resulting in a population of selection phage propagating in the flow of host cells in the lagoon. Phage-infected host cells were removed from the lagoon and fresh, uninfected host cells were fed into the lagoon at a rate resulting in the average time a host cell remained in the lagoon being shorter than the average time between cell division of the host cells, but longer than the average M13 phage life cycle time.

In order to generate Cas9 variants during a directed evolution experiment, the host cells in the lagoon were incubated under conditions resulting in an increased mutation rate. The host cells were harboring a mutagenesis plasmid (MP), which increased the mutagenesis rate, thus introducing mutations in the ω-dCas9 fusion protein encoded by the selection phagemid during the phage life cycle. Because the flow rate of host cells through the lagoon results in the average time a host cell remains in the lagoon being shorter than the average time between host cell divisions, the host cells in the lagoon cannot accumulate mutations resulting from the increased mutation rate conferred by the mutagenesis plasmid in their genome or on the accessory plasmid. The selection phage, however, replicate in the lagoon in the flow of host cells and thus accumulate mutations over time, resulting in the generation of new, evolved ω-dCas9 fusion protein variants.

If any of these evolved ω-dCas9 fusion protein variants includes a mutation that confers an increased activity on the accessory plasmid comprising the PAM library, this will directly translate into the generation of more pIII by a host cell infected with a selection phage encoding the respective ω-dCas9 fusion protein variant. The production of more pIII will, in turn, result in the generation of more infectious selection phage particles, which, over time, results in a competitive advantage of the mutant selection phage harboring such beneficial mutations over selection phage not harboring such mutations. After a period of time, the selective pressure exerted by the accessory plasmid will, therefore, result in selection phage having acquired beneficial mutations being the predominant species replicating in the flow of host cells, while selection phage with no mutations or with detrimental mutations will be washed out of the lagoon.

Because the ω-dCas9 fusion protein activity on the PAM library was very low at the beginning of the experiment, multiple rounds of overnight propagation of the selection phagemid in host cells harboring an accessory plasmid containing the PAM library were carried out to evolve Cas9 variants that show increased activity on noncanonical PAM sequences. At the end of a directed evolution experiment, the evolved population of selection phage was isolated from the lagoon, and a representative number of clones was analyzed to detect Cas9 variants having beneficial mutations. While all mutations observed confer a beneficial phenotype, mutations shared by more than one clone, or by all clones, are of particular interest.

Mutations from Cas9 PACE.

A number of selection phage clones was isolated from a directed evolution experiment using a PAM library accessory plasmid as described above. The mutations identified in the Cas9 amino acid sequence of some exemplary clones is provided below in Table 1 (residue numbering according to SEQ ID NO: 9):

TABLE 1 Clone Mutations 1 D182 E1219V 2 H137 D182 E1219V 3 D182 G660 E1219V 4 I122 D182 E1219V Cas9 mutations identified in PACE (residue numbering according to SEQ ID NO: 9.

Clones 1-4 were tested in the ω-dCas9 luciferase activation assay described above. When tested on the PAM library as a whole, the different clones showed an improvement in luciferase expression (FIG. 2—Cas9 activity of exemplary evolved clones on PAM library after directed evolution).

Improvement of Cas9 Activity on Non-Canonical PAM Sequences.

The activity of evolved Cas9 proteins on target sequences with noncanonical PAMs was evaluated in more detail. The relative activity of Clone 4, harboring 1122, D182, and E1219V mutations, for various [Doench 2 (5′-GGAGCCCACCGAGTACCTGG-3′ (SEQ ID NO: 287))]-[PAM] target sequences was tested in the ω-dCas9 luciferase activation assay and compared to the activity of wild-type dCas9.

Improvement of Cas9 Activity on Non-Canonical PAM Sequences.

The activity of evolved Cas9 proteins on target sequences with noncanonical PAMs was evaluated in more detail. The relative activity of Clone 4, harboring 1122, D182, and E1219V mutations, for various [Doench 2 (5′-GGAGCCCACCGAGTACCTGG-3′(SEQ ID NO: 287))]-[PAM] target sequences was tested in the ω-dCas9 luciferase activation assay and compared to the activity of wild-type dCas9. The data are shown in Table 2.

TABLE 2 Clone 4 dCas9 Wild-type dCas9 PAM Luciferase Activity Luciferase Activity GTC 44.84 4.07 AGC 25.01 4.42 GAG 186.22 73.60 TTT 36.49 3.96 GTG 119.53 6.86 AGG 238.29 244.27 CAA 13.90 5.28 Relative activity of clone 4 on various PAM sequences.

Example 2: PA CE Evolution of a Cas9 without any PAM Sequence Restriction

Because the activity of the ω-dCas9 fusion protein on the NNN-PAM libraries was very low, a second round of PACE experiments was performed, in which an initial phase of diversification of the ω-dCas9 fusion protein population was carried out in the absence of selective pressure by providing a source of pIII that did not depend on ω-dCas9 fusion protein activity. The initial diversification stage allows mutations to develop that may not be accessible in a PACE experiment where selective pressure is applied throughout the experiment.

Selection phage harboring a ω-dCas9 fusion protein with a dCas9 sequence provided as SEQ ID NO: 8 with D10A and H840A mutations was propagated overnight in 1030 host cells together with MP6 mutagenesis plasmid in the presence of arabinose in order to create a library of mutated selection phage encoding a library of ω-dCas9 fusion protein variants. PIII was expressed from a separate plasmid in the host cells during this initial diversification stage. After overnight (12 h) diversification, 1030 host cells harboring an accessory plasmid comprising an NNN PAM library cloned into a weak promoter as the guide RNA target sequence and a mutagenesis plasmid were grown to log phase, and used as the source of host cells to create a flow of host cells through a lagoon. The cells in the lagoon were infected with the diversified selection phage from the overnight incubation. The host cells in the lagoon were contacted with arabinose in order to maintain a high level of expression of the mutagenic genes from the mutagenesis plasmid.

The initial phage titers were about 10⁸ pfu/mL. A PACE experiment was performed for each of the four NNN-PAM libraries ([Doench 1]-[NNN-PAM], [Doench 2]-[NNN-PAM], [G7]-[NNN-PAM], and [VEGF target]-[NNN-PAM] cloned into an accessory plasmid driving pIII expression from a weak promoter as described above. The phage titers were monitored during the PACE experiments. A slow drop in phage titers to 10⁴ pfu/mL was observed. The phage population was isolated from the lagoon at that point, and grown on 2208 host cells containing a separate source of pIII (psp-driven pIII). After this low-stringency propagation period, a 1:100 dilution of the supernatant was added to fresh host cells harboring the accessory plasmid as the only source of pIII in a new lagoon, and the PACE experiments were continued. No drop in phage titers was observed after this low-stringency incubation in 2208 cells.

One exemplary PACE experiment that was run for 72 hours. After that period of time, 24 surviving clones were isolated from the lagoon, sequenced, and characterized. The mutations identified included A262T, K294R, S409I, M694I, E480K, E543D, and E1219V (amino acid numbering according to SEQ ID NO: 9). In another exemplary experiment, surviving clones were isolated after 15 days of incubation. Activity of the identified dVas9 mutants was characterized in a ω-dCas9 luciferase assay. Clones with the best ω-dCas9 fusion protein activity on noncanonical PAM target sequences had the following mutations: E480K, E543D, E1219V, and T1329.

Cas9 Mammalian GFP Activation.

Both wild-type dCas9 (SEQ ID NO: 9) and the evolved Cas9 clones were tested in a dCas9-GFP assay in Hek293T cells. The cells were contacted with a reporter construct in which a GFP-encoding sequence was driven from a weak promoter that includes a [gRNA target sequence]-[PAM] sequence. Fusion proteins of dCas9 (wild-type and PACE variants) attached to the transcriptional activator VP64-p65-Rta (VPR) were generated, and the various dCas9-VPR variants were tested for their capacity to activate the GFP reporter in HEK293 cells.

Hek293T were transfected with four separate plasmids: a dCas9-VPR expression plasmid; a plasmid expressing the sgRNA targeting the gRNA target sequence of the GFP reporter plasmid; the GFP reporter plasmid; and an iRFP transfection control. In one experiment, the HEK293 cells were contacted with a GFP reporter included a TAA PAM, and in another experiment, the HEK293 cells were contacted with a population of reporter plasmids containing an NNN PAM library. Cells were harvested 48 hours after transfection and GFP expressing cells were quantified using a BD LSR-FORTESSA cell analyzer.

FIG. 3—Cas9 Mammalian GFP Activation. Compared with WT Cas9, the evolved Cas9s showed much higher activity on both the TAA PAM (21.08% vs. 0.60% of cells above negative control) and a library of NNN PAM (22.76% vs. 3.38% of cells above negative control).

Evolved Cas9 Cutting Activity on Target Sequences with Noncanonical PAM.

In order to demonstrate that the PACE mutations universally confer Cas9 activity without PAM restriction, nuclease-active Cas9 proteins were generated based on the sequence provided in, i.e., without the D10A and H840A mutations, but harboring the various PACE mutations. The evolved Cas9 variants were tested in a Cas9 GFP assay, assessing the capacity of the evolved Cas9 protein variants to target and inactivate an emGFP gene integrated into the genome of HEK293 cells using a guide RNA targeting a sequence with a noncanonical PAM. It was observed that 6.45% of cells showed loss of GFP expression when contacted with a wild-type nuclease-active Cas9 (SEQ ID NO: 9), while 54.55% of cells contacted with the evolved Cas9 (E480K, E543D, E1219V, and T1329) showed a loss of GFP expression.

Example 3: Cas9 Variants without PAM Restrictions

The beneficial mutations conferring Cas9 activity on noncanonical PAM sequences were mapped to a S. pyogenes wild-type sequence. Below is an exemplary Cas9 sequence (S. pyogenes Cas9 with D10 and H840 residues marked with an asterisk following the respective amino acid residues, SEQ ID NO: 9). The D10 and H840 residues of SEQ ID NO: 9 may be mutated to generate a nuclease inactive Cas9 (e.g., to D10A and H840A) or to generate a nickase Cas9 (e.g., to D10A with H840; or to D10 with H840A). The HNH domain (bold and underlined) and the RuvC domain (boxed) are identified. The residues found mutated in the clones isolated from the various PACE experiments, amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 are identified with an asterisk following the respective amino acid residue.

(SEQ ID NO: 9) MDKK YSIGLD*IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGET AEATRLKRTARRRYTR RKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI*VDEVAYHEKYPTIYH*LRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVD*KLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRR LENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA*KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK* NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTEDNGS*IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE*VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGE*QKKAIVDLLFKTNRKVTVKQLKEDYFKKIECEDSVEISGVEDRFNASL GTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWG*RLSRK

YLQNGRDMYVDQELDINRLSDYDVDH*IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLN

RNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYK EVKKDLIIKLPKYSLFELENGRKRMLASAGE*LQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDT*TIDRKRY TSTKEVLDATLIHQSITGLYETRIDLSQLGGD

The beneficial mutations conferring Cas9 activity on noncanonical PAM sequences were mapped to additional exemplary wild-type Cas9 sequences. The HNH domain (bold and underlined) and the RuvC domain (boxed) are identified. The residues homologous to the residues found mutated in SEQ ID NO: 9, amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 are identified with an asterisk following the respective amino acid residue. In addition, amino acid residues 10 and 840, which are mutated in dCas9 protein variants, are also identified by an asterisk.

(SEQ ID NO: 297) MDKK YSIGLD*IGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFGSGET AEATRLKRTARRRYTR RKNRICYLQEIFSNEMAKVDDSFEHRLEESFLVEEDKKHERHPIEGNI*VDEVAYHEKYPTIYH*LRKKLADSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVD*KLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRR LENLIAQLPGEKRNGLEGNLIALSLGLIPNFKSNFDLAEDA*KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK* NLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS*IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE*VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGE*QKKAIVDLLFKTNRKVTVKQLKEDYFKKIECEDSVEISGVEDRFNASL GAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEERLKTYAHLFDDKVMKQLKRRRYTGWG*RLSRK

LQNGRDMYVDQELDINRLSDYDVDH*IVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNA

NSDKLIARKKDWDPKKYGGFDSPIVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKE VKKDLIIKLPKYSLFELENGRKRMLASAGE*LQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLINLGAPAAFKYFDT*TIDRKRYT STKEVLDATLIHQSITGLYETRIDLSQLGGD (SEQ ID NO: 298) MDKK YSIGLA*IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGET AEATRLKRTARRRYTR RKNRICYLQEIFSNEMAKVDDSFFHRLEESELVEEDKKHERHPIFGNI*VDEVAYHEKYPTIYH*LRKKLVDSTD KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVD*KLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRR LENLIAQLPGEKKNGLFGNLIALSLGLIPNFKSNFDLAEDA*KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK* NLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTEDNGS*IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE*VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGE*QKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASL GTYHDLLKIIKDKDFLDNEENEDILEDIVLILTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWG*RLSRK

YLQNGRDMYVDQELDINRLSDYDVDH*IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLN

RNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYK EVKKDLIIKLPKYSLFELENGRKRMLASAGE*LQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLINLGAPAAFKYFDT*TIDRKRY TSTKEVLDATLIHQSITGLYETRIDLSQLGGD >gi|504540549|ref|WP_014727651.1| type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus thermophilus] (SEQ ID NO: 260) MTKP YSIGLD*IGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLLFDSGIT AEGRRLKRTARRRYTR RRNRILYLQEIFSTEMATLDDAFFQRLDDSFLVPDDKRDSKYPIFGNL*VEEKAYHDEEPTIYH*LRKYLADSTK KADLRLVYLALAHMIKYRGHFLIEGEFNSKNNDIQ*KNFQDFLDTYNAIFESDLSLENSKQLEEIVKDKISKLEK KDRILKLFPGEKNSGIFSEFLKLIVGNQADFRKCFNLDEKA*SLHFSKESYDEDLETLLGYIGDDYSDVFLKAK* KLYDAILLSGFLTVTDNETEAPLSSAMIKRYNEHKEDLALLKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQ EDFYVYLKNLLAEFEGADYFLEKIDREDFLRKQRTEDNGS*IPYQIHLQEMRAILDKQAKFYPFLAKNKERIEKI LTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFED*VIDKESSAEAFINRMTSFDLYLPEEKVLPKHSLLYET FNVYNELTKVRFIAESMRDYQFLDSK*QKKDIVRLYFKDKRKVTDKDIIEYLHAIYGYDGIELKGIEKQFNSSLS TYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQRLSKFENIFDKSVLKKLSRRHYTGWG*KLSAKL

NDRLYLYYLQNGKDMYTGDDLDIDRLSNYDIDH*IIPQAFLKDNSIDNKVLVSSASNRGKSDDFPSLEVVKKRKT

FNANLSSKPKPNSNENLVGAKEYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRINYRKD KLNFLLEKGYKDIELIIELPKYSLFELSDGSRRMLASILSTNNKRGE*IHKGNQIFLSQKFVKLLYHAKRISNTI NENHRKYVENHKKEFEELFYYILEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFELTSR GSAADFEFLGV*KIPRYRDYTPSSLLKDATLIHQSVTGLYETRIDLAKLGEG >gi 924443546 | Staphylococcus Aureus Cas9 (SEQ ID NO: 261) GSHMKRN YILGLD*IGITSVGYGIIDYETRDVIDAGVRLFKEANVEN NEGRRSKRGARRLKRRRRHRIQRVKKLL FDYNLLTDHSELSGINP*YEARVKGLSQKLSEEEFSAALLHLAKRRGVHNVNEVEEDTGNELST*KEQISRNSKA LEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGS PFGWKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYY*EKFQIIENVEKQKKK PTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAKILTIYQSSEDIQEEL

AKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDH*IIPRSVSFDNSFNNKVLVKQEEASKKGNRTPFQ

LKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAH LDITDDYPNS*RNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKAYEEAKKLKKISNQAEFIA SFYNNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLEN*MNDKRPPRIIKTIASKTQSIKKYSTDILGNL YEVKSKKHPQIIKKG

This disclosure provides Cas9 variants in which one or more of the amino acid residues identified by an asterisk are mutated as described herein. In some embodiments, the D10 and H840 residues are mutated, e.g., to an alanine residue, and the Cas9 variants provided include one or more additional mutations of the amino acid residues identified by an asterisk as provided herein. In some embodiments, the D10 residue is mutated, e.g., to an alanine residue, and the Cas9 variants provided include one or more additional mutations of the amino acid residues identified by an asterisk as provided herein.

A number of Cas9 sequences from various species were aligned to determine whether corresponding homologous amino acid residues can be identified in other Cas9 proteins, allowing the generation of Cas9 variants with corresponding mutations of the homologous amino acid residues. The alignment was carried out using the NCBI Constraint-based Multiple Alignment Tool (COBALT (accessible at st-va.ncbi.nlm.nih.gov/tools/cobalt), with the following parameters. Alignment parameters: Gap penalties −11, −1; End-Gap penalties −5, −1. CDD Parameters: Use RPS BLAST on; Blast E-value 0.003; Find Conserved columns and Recompute on. Query Clustering Parameters: Use query clusters on; Word Size 4; Max cluster distance 0.8; Alphabet Regular.

An exemplary alignment of four Cas9 sequences is provided below. The Cas9 sequences in the alignment are: Sequence 1 (S1): SEQ ID NO: 10|WP_010922251|gi 499224711|type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes]; Sequence 2 (S2): SEQ ID NO: 11|WP_039695303|gi 746743737|type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus gallolyticus]; Sequence 3 (S3): SEQ ID NO: 12|WP_045635197|gi 782887988|type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mitis]; Sequence 4 (S4): SEQ ID NO: 13|5AXW_A|gi 924443546|Staphylococcus Aureus Cas9. The HNH domain (bold and underlined) and the RuvC domain (boxed) are identified for each of the four sequences. Amino acid residues 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and 1329 in S1 and the homologous amino acids in the aligned sequences are identified with an asterisk following the respective amino acid residue.

S1 1 --MDKK- YSIGLD*IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI--GALLFDSG--ET AEATRLKRTARRRYT 73 S2 1 --MTKKN YSIGLD*IGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLL--GALLFDSG--ET AEATRLKRTARRRYT 74 S3 1 --M-KKG YSIGLD*IGTNSVGFAVITDDYKVPSKKMKVLGNTDKRFIKKNLI--GALLFDEG--TT AEARRLKRTARRRYT 73 S4 1 GSHMKRN YILGLD*IGITSVGYGII--DYET-----------------RDVIDAGVRLFKEANVEN NEGRRSKRGARRLKR 61 S1 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEESELVEEDKKHERHPIFGNI*VDEVAYHEKYPTIYH*LRKKLVDSTDKADLRL 153 S2 75 RRKNRLRYLQEIFANEIAKVDESFFQRLDESFLTDDDKTFDSHPIFGNK*AEEDAYHQKFPTIYH*LRKHLADSSEKADLRL 154 S3 74 RRKNRLRYLQEIFSEEMSKVDSSFFHRLDDSFLIPEDKRESKYPIFATL*TEEKEYHKQFPTIYH*LRKQLADSKEKTDLRL 153 S4 62 RRRHRIQRVKKLL--------------FDYNLLTD---------------------HSELSGINP*YEARVKGLSQKLSEEE 107 S1 154 IYLALAHMIKFRGHFLIEGDLNPDNSDVD*KLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEK 233 S2 155 VYLALAHMIKFRGHFLIEGELNAENTDVQ*KIFADFVGVYNRTFDDSHLSEITVDVASILTEKISKSRRLENLIKYYPTEK 234 S3 154 IYLALAHMIKYRGHFLYEEAFDIKNNDIQ*KIFNEFISIYDNTFEGSSLSGQNAQVEAIFTDKISKSAKRERVLKLFPDEK 233 S4 108 FSAALLHLAKRRG-----------------------VHNVNEVEEDT---------------------------------- 131 S1 234 KNGLFGNLIALSLGLTPNFKSNFDLAEDA*KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAK*NLSDAILLSDILRVNTEIT 313 S2 235 KNTLFGNLIALALGLQPNFKTNFKLSEDA*KLQFSKDTYEEDLEELLGKIGDDYADLFTSAK*NLYDAILLSGILTVDDNST 314 S3 234 STGLFSEFLKLIVGNQADFKKHFDLEDKA*PLQFSKDTYDEDLENLLGQIGDDFTDLFVSAK*KLYDAILLSGILTVTDPST 313 S4 132 -----GNELS------------------T*KEQISRN--------------------------------------------- 144 S1 314 KAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM--DGTEELLV 391 S2 315 KAPLSASMIKRYVEHHEDLEKLKEFIKANKSELYHDIFKDKNKNGYAGYIENGVKQDEFYKYLKNILSKIKIDGSDYFLD 394 S3 314 KAPLSASMIERYENHQNDLAALKQFIKNNLPEKYDEVFSDQSKDGYAGYIDGKTTQETFYKYIKNLLSKF--EGTDYFLD 391 S4 145 ----SKALEEKYVAELQ-------------------------------------------------LERLKKDG------ 165 S1 392 KLNREDLLRKQRTFDNGS*IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEE 471 S2 395 KIEREDFLRKQRTFDNGS*IPHQIHLQEMHAILRRQGDYYPFLKEKQDRIEKILTFRIPYYVGPLVRKDSRFAWAEYRSDE 474 S3 392 KIEREDFLRKQRTFDNGS*IPHQIHLQEMNAILRRQGEYYPFLKDNKEKIEKILTFRIPYYVGPLARGNRDFAWLTRNSDE 471 S4 166 --EVRGSINRFKTSD---------YVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGP--GEGSPFGW------K 227 S1 472 TITPWNFEE*VVDKGASAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGE*QKKAIVDL 551 S2 475 KITPWNFDK*VIDKEKSAEKFITRMTLNDLYLPEEKVLPKHSHVYETYAVYNELTKIKYVNEQGKE-SFFDSN*MKQEIFDH 553 S3 472 AIRPWNFEE*IVDKASSAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIAEGLRDYQFLDSG*QKKQIVNQ 551 S4 228 DIKEW----------------YEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEK---LEYY*EKFQIIEN 289 S1 552 LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDR---FNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFED 628 S2 554 VFKENRKVTKEKLLNYLNKEFPEYRIKDLIGLDKENKSFNASLGTYHDLKKIL-DKAFLDDKVNEEVIEDIIKTLTLFED 632 S3 552 LFKENRKVTEKDIIHYLHN-VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDKEFMDDAKNEAILENIVHTLTIFED 627 S4 290 VFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEF---TNLKVYHDIKDITARKEII---ENAELLDQIAKILTIYQS 363 S1 629 REMIEERLKTYAHLFDDKVMKQLKR-RRYTGWG*RLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM*QLIHDDSLTEKED 707 S2 633 KDMIHERLQKYSDIFTANQLKKLER-RHYTGWG*RLSYKLINGIRNKENNKTILDYLIDDGSANRNFM*QLINDDTLPFKQI 711 S3 628 REMIKQRLAQYDSLFDEKVIKALTR-RHYTGWG*KLSAKLINGICDKQTGNTILDYLIDDGKINRNFM*QLINDDGLSFKEI 706 S4 364 SEDIQEELTNLNSELTQEEIEQISNLKGYTGTH*NLSLKAINLILDE------LWHTNDNQIAIFNRL*KLVP--------- 428 S1 708

781 S2 712

784 S3 707

779 S4 429

505 S1 782 KRIEEGIKELGSQIL-------KEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSD----YDVDH*IVPQSFLKDD 850 S2 785 KKLQNSLKELGSNILNEEKPSYIEDKVENSHLQNDQLFLYYIQNGKDMYTGDELDIDHLSD----YDIDH*IIPQAFIKDD 860 S3 780 KRIEDSLKILASGL---DSNILKENPTDNNQLQNDRLFLYYLQNGKDMYTGEALDINQLSS----YDIDH*IIPQAFIKDD 852 S4 506 ERIEEIIRTTGK---------------ENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDH*IIPRSVSFDN 570 S1 851

922 S2 861

932 S3 853

924 S4 571

650 S1 923

1002 S2 933

1012 S3 925

1004 S4 651

712 S1 1003

1077 S2 1013 

1083 S3 1005 

1081 S4 713

764 S1 1078

1149 S2 1084

1158 S3 1082

1156 S4 765

835 S1 1150 EKGKSKKLKSVKELLGITIMERSSFEKNPI-DFLEAKG-----YKEVKKDLIIKLPKYSLFELENGRKRMLASAGE*LQKG 1223 S2 1159 EKGKAKKLKTVKELVGISIMERSFFEENPV-EFLENKG-----YHNIREDKLIKLPKYSLFEFEGGRRRLLASASE*LQKG 1232 S3 1157 EKGKAKKLKTVKTLVGITIMEKAAFEENPI-TFLENKG-----YHNVRKENILCLPKYSLFELENGRRRLLASAKE*LQKG 1230 S4 836 DPQTYQKLK--------LIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNS*RNKV 907 S1 1224 NELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKH------ 1297 S2 1233 NEMVLPGYLVELLYHAHRADNF-----NSTEYLNYVSEHKKEFEKVLSCVEDFANLYVDVEKNLSKIRAVADSM------ 1301 S3 1231 NEIVLPVYLTTLLYHSKNVHKL-----DEPGHLEYIQKHRNEFKDLLNLVSEFSQKYVLADANLEKIKSLYADN------ 1299 S4 908 VKLSLKPYRFD-VYLDNGVYKFV-----TVKNLDVIK--KENYYEVNSKAYEEAKKLKKISNQAEFIASFYNNDLIKING 979 S1 1298 RDKPIREQAENIIHLFTLTNLGAPAAFKYFDT*TIDRKRYTSTKEVLDATLIHQSIT--------GLYETRI----DLSQL 1365 S2 1302 DNFSIEEISNSFINLLTLTALGAPADFNFLGE*KIPRKRYTSTKECLNATLIHQSIT--------GLYETRI----DLSKL 1369 S3 1300 EQADIEILANSFINLLTFTALGAPAAFKFFGK*DIDRKRYTTVSEILNATLIHQSIT--------GLYETWI----DLSKL 1367 S4 980 ELYRVIGVNNDLLNRIEVNMIDITYR-EYLEN*MNDKRPPRIIKTIASKT---QSIKKYSTDILGNLYEVKSKKHPQIIKK 1055 S1 1366 GGD 1368 S2 1370 GEE 1372 S3 1368 GED 1370 S4 1056 G-- 1056

The alignment demonstrates that amino acid sequences and amino acid residues that are homologous to a reference Cas9 amino acid sequence or amino acid residue can be identified across Cas9 sequence variants, including, but not limited to Cas9 sequences from different species, by identifying the amino acid sequence or residue that aligns with the reference sequence or the reference residue using alignment programs and algorithms known in the art. This disclosure provides Cas9 variants in which one or more of the amino acid residues identified by an asterisk in SEQ ID NO: 9 are mutated as described herein. The residues in Cas9 sequences other than SEQ ID NO: 9 that correspond to the residues identified in SEQ ID NO: 9 by an asterisk are referred to herein as “homologous” or “corresponding” residues. Such homologous residues can be identified by sequence alignment, e.g., as described above, and by identifying the sequence or residue that aligns with the reference sequence or residue. Similarly, mutations in Cas9 sequences other than SEQ ID NO: 9 that correspond to mutations identified in SEQ ID NO: 9 herein, e.g., mutations of residues 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and 1329 in SEQ ID NO: 9, are referred to herein as “homologous” or “corresponding” mutations. For example, the mutations corresponding to the D10A mutation in S1 for the four aligned sequences above are D10A for S2, D9A for S3, and D13A for S4; the corresponding mutations for H840A in S1 are H850A for S2, H842A for S3, and H560 for S4; the corresponding mutation for X1219V in S1 are X1228V for S2, X1226 for S3, and X903V for S4, and so on.

A total of 250 Cas9 sequences (SEQ ID NOs: 10-262) from different species were aligned using the same algorithm and alignment parameters outlined above. Amino acid residues homologous to residues 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and 1329 were identified in the same manner as outlined above. The alignments are provided below. The HNH domain (bold and underlined) and the RuvC domain (boxed) are identified for each of the four sequences. Residues corresponding to amino acid residues 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and 1329 in SEQ ID NO: 9 are boxed in SEQ ID NO: 10 in the alignments, allowing for the identification of the corresponding amino acid residues in the aligned sequences.

Cas9 variants with one or more mutations in amino acid residues homologous to amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of SEQ ID NO: 9 are provided herein. In some embodiments, the Cas9 variants provided herein comprise mutations corresponding to the D10A and the H840A mutations in SEQ ID NO: 9, resulting in a nuclease-inactive dCas9, and at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations of amino acid residues homologous to amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of SEQ ID NO: 9.

Cas9 variants with one or more mutations in amino acid residues homologous to amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of SEQ ID NO: 9 are provided herein. In some embodiments, the Cas9 variants provided herein comprise mutations corresponding to the D10A mutations in SEQ ID NO: 9, resulting in a partially nuclease-inactive dCas9, wherein the Cas9 can nick the non-target strand but not the targeted strand, and at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations of amino acid residues homologous to amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of SEQ ID NO: 9.

WP_010922251.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 10 WP_039695303.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus gallolyticus] SEQ ID NO: 11 WP_045635197.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mitis] SEQ ID NO: 12 5AXW_A Cas9, Chain A, Crystal Structure [Staphylococcus aureus] SEQ ID NO: 13 WP_009880683.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 14 WP_010922251.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 15 WP_011054416.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 16 WP_011284745.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 17 WP_011285506.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 18 WP_011527619.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 19 WP_012560673.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 20 WP_014407541.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 21 WP_020905136.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 22 WP_023080005.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 23 WP_023610282.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 24 WP_030125963.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 25 WP_030126706.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 26 WP_031488318.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 27 WP_032460140.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 28 WP_032461047.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 29 WP_032462016.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 30 WP_032462936.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 31 WP_032464890.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 32 WP_033888930.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 33 WP_038431314.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 34 WP_038432938.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 35 WP_038434062.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] SEQ ID NO: 36 BAQ51233.1 CRISPR-associated protein, Csn1 family [Streptococcus pyogenes] SEQ ID NO: 37 KGE60162.1 hypothetical protein MGA52111_0903 [Streptococcus pyogenes MGAS2111] SEQ ID NO: 38 KGE60856.1 CRISPR-associated endonuclease protein [Streptococcus pyogenes SS1447] SEQ ID NO: 39 WP_002989955.1 MULTISPECIES: type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus] SEQ ID NO: 40 WP_003030002.1 MULTISPECIES: type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus] SEQ ID NO: 41 WP_003065552.1 MULTISPECIES: type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus] SEQ ID NO: 42 WP_001040076.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 43 WP_001040078.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 44 WP_001040080.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 45 WP_001040081.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 46 WP_001040083.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 47 WP_001040085.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 48 WP_001040087.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 49 WP_001040088.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 50 WP_001040089.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 51 WP_001040090.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 52 WP_001040091.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 53 WP_001040092.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 54 WP_001040094.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 55 WP_001040095.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 56 WP_001040096.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 57 WP_001040097.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 58 WP_001040098.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 59 WP_001040099.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 60 WP_001040100.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 61 WP_001040104.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 62 WP_001040105.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 63 WP_001040106.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 64 WP_001040107.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 65 WP_001040108.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 66 WP_001040109.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 67 WP_001040110.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 68 WP_015058523.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 69 WP_017643650.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 70 WP_017647151.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 71 WP_017648376.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 72 WP_017649527.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 73 WP_017771611.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 74 WP_017771984.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 75 CFQ25032.1 CRISPR-associated protein [Streptococcus agalactiae] SEQ ID NO: 76 CFV16040.1 CRISPR-associated protein [Streptococcus agalactiae] SEQ ID NO: 77 KLJ37842.1 CRISPR-associated protein Csn1 [Streptococcus agalactiae] SEQ ID NO: 78 KLJ72361.1 CRISPR-associated protein Csn1 [Streptococcus agalactiae] SEQ ID NO: 79 KLL20707.1 CRISPR-associated protein Csn1 [Streptococcus agalactiae] SEQ ID NO: 80 KLL42645.1 CRISPR-associated protein Csn1 [Streptococcus agalactiae] SEQ ID NO: 81 WP_047207273.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 82 WP_047209694.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 83 WP_050198062.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 84 WP_050201642.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 85 WP_050204027.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 86 WP_050881965.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 87 WP_050886065.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus agalactiae] SEQ ID NO: 88 AHN30376.1 CRISPR-associated protein Csn1 [Streptococcus agalactiae 138P] SEQ ID NO: 89 EAO78426.1 reticulocyte binding protein [Streptococcus agalactiae H36B] SEQ ID NO: 90 CCW42055.1 CRISPR-associated protein, SAG0894 family [Streptococcus agalactiae ILRI112] SEQ ID NO: 91 WP_003041502.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus anginosus] SEQ ID NO: 92 WP_037593752.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus anginosus] SEQ ID NO: 93 WP_049516684.1 CRISPR-associated protein Csn1 [Streptococcus anginosus] SEQ ID NO: 94 GAD46167.1 hypothetical protein ANG6_0662 [Streptococcus anginosus T5] SEQ ID NO: 95 WP_018363470.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus caballi] SEQ ID NO: 96 WP_003043819.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus canis] SEQ ID NO: 97 WP_006269658.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus constellatus] SEQ ID NO: 98 WP_048800889.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus constellatus] SEQ ID NO: 99 WP_012767106.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus dysgalactiae] SEQ ID NO: 100 WP_014612333.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus dysgalactiae] SEQ ID NO: 101 WP_015017095.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus dysgalactiae] SEQ ID NO: 102 WP_015057649.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus dysgalactiae] SEQ ID NO: 103 WP_048327215.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus dysgalactiae] SEQ ID NO: 104 WP_049519324.1 CRISPR-associated protein Csn1 [Streptococcus dysgalactiae] SEQ ID NO: 105 WP_012515931.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus equi] SEQ ID NO: 106 WP_021320964.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus equi] SEQ ID NO: 107 WP_037581760.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus equi] SEQ ID NO: 108 WP_004232481.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus equinus] SEQ ID NO: 109 WP_009854540.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus gallolyticus] SEQ ID NO: 110 WP_012962174.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus gallolyticus] SEQ ID NO: 111 WP_039695303.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus gallolyticus] SEQ ID NO: 112 WP_014334983.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus infantarius] SEQ ID NO: 113 WP_003099269.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus iniae] SEQ ID NO: 114 AHY15608.1 CRISPR-associated protein Csn1 [Streptococcus iniae] SEQ ID NO: 115 AHY17476.1 CRISPR-associated protein Csn1 [Streptococcus iniae] SEQ ID NO: 116 ESR09100.1 hypothetical protein IUSA1_08595 [Streptococcus iniae IUSA1] SEQ ID NO: 117 AGM98575.1 CRISPR-associated protein Cas9/Csn1, subtype II/NMEMI [Streptococcus iniae SF1] SEQ ID NO: 118 ALF27331.1 CRISPR-associated protein Csn1 [Streptococcus intermedius] SEQ ID NO: 119 WP_018372492.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus massiliensis] SEQ ID NO: 120 WP_045618028.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mitis] SEQ ID NO: 121 WP_045635197.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mitis] SEQ ID NO: 122 WP_002263549.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 123 WP_002263887.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 124 WP_002264920.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 125 WP_002269043.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 126 WP_002269448.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 127 WP_002271977.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 128 WP_002272766.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 129 WP_002273241.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 130 WP_002275430.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 131 WP_002276448.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 132 WP_002277050.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 133 WP_002277364.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 134 WP_002279025.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 135 WP_002279859.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 136 WP_002280230.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 137 WP_002281696.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 138 WP_002282247.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 139 WP_002282906.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 140 WP_002283846.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 141 WP_002287255.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 142 WP_002288990.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 143 WP_002289641.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 144 WP_002290427.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 145 WP_002295753.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 146 WP_002296423.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 147 WP_002304487.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 148 WP_002305844.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 149 WP_002307203.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 150 WP_002310390.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 151 WP_002352408.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 152 WP_012997688.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 153 WP_014677909.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 154 WP_019312892.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 155 WP_019313659.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 156 WP_019314093.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 157 WP_019315370.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 158 WP_019803776.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 159 WP_019805234.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 160 WP_024783594.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 161 WP_024784288.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 162 WP_024784666.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 163 WP_024784894.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 164 WP_024786433.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus mutans] SEQ ID NO: 165 WP_049473442.1 CRISPR-associated protein Csn1 [Streptococcus mutans] SEQ ID NO: 166 WP_049474547.1 CRISPR-associated protein Csn1 [Streptococcus mutans] SEQ ID NO: 167 EMC03581.1 hypothetical protein SMU69_09359 [Streptococcus mutans NLML4] SEQ ID NO: 168 WP_000428612.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus oralis] SEQ ID NO: 169 WP_000428613.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus oralis] SEQ ID NO: 170 WP_049523028.1 CRISPR-associated protein Csn1 [Streptococcus parasanguinis] SEQ ID NO: 171 WP_003107102.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus parauberis] SEQ ID NO: 172 WP_054279288.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus phocae] SEQ ID NO: 173 WP_049531101.1 CRISPR-associated protein Csn1 [Streptococcus pseudopneumoniae] SEQ ID NO: 174 WP_049538452.1 CRISPR-associated protein Csn1 [Streptococcus pseudopneumoniae] SEQ ID NO: 175 WP_049549711.1 CRISPR-associated protein Csn1 [Streptococcus pseudopneumoniae] SEQ ID NO: 176 WP_007896501.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pseudoporcinus] SEQ ID NO: 177 EFR44625.1 CRISPR-associated protein, Csn1 family [Streptococcus pseudoporcinus SPIN 20026] SEQ ID NO: 178 WP_002897477.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus sanguinis] SEQ ID NO: 179 WP_002906454.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus sanguinis] SEQ ID NO: 180 WP_009729476.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus sp. F0441] SEQ ID NO: 181 CQR24647.1 CRISPR-associated protein [Streptococcus sp. FF10] SEQ ID NO: 182 WP_000066813.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus sp. M334] SEQ ID NO: 183 WP_009754323.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus sp. taxon 056] SEQ ID NO: 184 WP_044674937.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus suis] SEQ ID NO: 185 WP_044676715.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus suis] SEQ ID NO: 186 WP_044680361.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus suis] SEQ ID NO: 187 WP_044681799.1 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus suis] SEQ ID NO: 188 WP_049533112.1 CRISPR-associated protein Csn1 [Streptococcus suis] SEQ ID NO: 189 WP_029090905.1 type II CRISPR RNA-guided endonuclease Cas9 [Brochothrix thermosphacta] SEQ ID NO: 190 WP_006506696.1 type II CRISPR RNA-guided endonuclease Cas9 [Catenibacterium mitsuokai] SEQ ID NO: 191 AIT42264.1 Cas9hc:NLS:HA [Cloning vector pYB196] SEQ ID NO: 192 WP_034440723.1 type II CRISPR endonuclease Cas9 [Clostridiales bacterium S5-A11] SEQ ID NO: 193 AKQ21048.1 Cas9 [CRISPR-mediated gene targeting vector p(bh5p68-Cas9)] SEQ ID NO: 194 WP_004636532.1 type II CRISPR RNA-guided endonuclease Cas9 [Dolosigranulum pigrum] SEQ ID NO: 195 WP_002364836.1 MULTISPECIES: type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus] SEQ ID NO: 196 WP_016631044.1 MULTISPECIES: type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus] SEQ ID NO: 197 EM575795.1 hypothetical protein H318_06676 [Enterococcus durans IPLA 655] SEQ ID NO: 198 WP_002373311.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 199 WP_002378009.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 200 WP_002407324.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 201 WP_002413717.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 202 WP_010775580.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 203 WP_010818269.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 204 WP_010824395.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 205 WP_016622645.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 206 WP_033624816.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 207 WP_033625576.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 208 WP_033789179.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecalis] SEQ ID NO: 209 WP_002310644.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 210 WP_002312694.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 211 WP_002314015.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 212 WP_002320716.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 213 WP_002330729.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 214 WP_002335161.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 215 WP_002345439.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 216 WP_034867970.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 217 WP_047937432.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus faecium] SEQ ID NO: 218 WP_010720994.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus hirae] SEQ ID NO: 219 WP_010737004.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus hirae] SEQ ID NO: 220 WP_034700478.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus hirae] SEQ ID NO: 221 WP_007209003.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus italicus] SEQ ID NO: 222 WP_023519017.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus mundtii] SEQ ID NO: 223 WP_010770040.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus phoeniculicola] SEQ ID NO: 224 WP_048604708.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus sp. AM1] SEQ ID NO: 225 WP_010750235.1 type II CRISPR RNA-guided endonuclease Cas9 [Enterococcus villorum] SEQ ID NO: 226 AII16583.1 Cas9 endonuclease [Expression vector pCas9] SEQ ID NO: 227 WP_029073316.1 type II CRISPR RNA-guided endonuclease Cas9 [Kandleria vitulina] SEQ ID NO: 228 WP_031589969.1 type II CRISPR RNA-guided endonuclease Cas9 [Kandleria vitulina] SEQ ID NO: 229 KDA45870.1 CRISPR-associated protein Cas9/Csn1, subtype II/NMEMI [Lactobacillus animalis] SEQ ID NO: 230 WP_039099354.1 type II CRISPR RNA-guided endonuclease Cas9 [Lactobacillus curvatus] SEQ ID NO: 231 AKP02966.1 hypothetical protein ABB45 04605 [Lactobacillus farciminis] SEQ ID NO: 232 WP_010991369.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria innocua] SEQ ID NO: 233 WP_033838504.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria innocua] SEQ ID NO: 234 EHN60060.1 CRISPR-associated protein, Csn1 family [Listeria innocua ATCC 33091] SEQ ID NO: 235 EFR89594.1 crispr-associated protein, Csn1 family [Listeria innocua FSL S4-378] SEQ ID NO: 236 WP_038409211.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria ivanovii] SEQ ID NO: 237 EFR95520.1 crispr-associated protein Csn1 [Listeria ivanovii FSL F6-596] SEQ ID NO: 238 WP_003723650.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 239 WP_003727705.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 240 WP_003730785.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 241 WP_003733029.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 242 WP_003739838.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 243 WP_014601172.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 244 WP_023548323.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 245 WP_031665337.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 246 WP_031669209.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 247 WP_033920898.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria monocytogenes] SEQ ID NO: 248 AKI42028.1 CRISPR-associated protein [Listeria monocytogenes] SEQ ID NO: 249 AKI50529.1 CRISPR-associated protein [Listeria monocytogenes] SEQ ID NO: 250 EFR83390.1 crispr-associated protein Csn1 [Listeria monocytogenes FSL F2-208] SEQ ID NO: 251 WP_046323366.1 type II CRISPR RNA-guided endonuclease Cas9 [Listeria seeligeri] SEQ ID NO: 252 AKE81011.1 Cas9 [Plant multiplex genome editing vector pYLCRISPR/Cas9Pubi-H] SEQ ID NO: 253 CUO82355.1 Uncharacterized protein conserved in bacteria [Roseburia hominis] SEQ ID NO: 254 WP_033162887.1 type II CRISPR RNA-guided endonuclease Cas9 [Sharpea azabuensis] SEQ ID NO: 255 AGZ01981.1 Cas9 endonuclease [synthetic construct] SEQ ID NO: 256 AKA60242.1 nuclease deficient Cas9 [synthetic construct] SEQ ID NO: 257 AK540380.1 Cas9 [Synthetic plasmid pFC330] SEQ ID NO: 258 4UN5_B Cas9, Chain B, Crystal Structure SEQ ID NO: 259 WP_010922251 1

73 WP_039695303 1 MTKKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_045635197 1 K-KG-YSIGLDIGTNSVGFAVITDDYKVPSKKMKVLGNTDKRFIKKNLIGALLFDEGTTA--EARRLKRTARRRYT 73 5AXW_A 1 MKRN-YILGLDIGITSVGYGII--DYET------------RDVIDA---GVRLFKEANVEnnEGRRSKRGARRLKR 61 WP_009880683 ---------------------------------------------------------------------------- WP_010922251 1 MDKK-YSIGLDIGINSVGWAVITDEYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_011054416 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKLKGLGNIDRHGIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_011284745 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_011285506 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_011527619 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGEIA--EATRLKRTARRRYT 73 WP_012560673 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_014407541 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFGSGETA--EATRLKRTARRRYT 73 WP_020905136 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_023080005 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKLKVLGNIDRHGIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_023610282 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKLKVLGNIDRHGIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_030125963 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_030126706 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHGIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_031488318 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_032460140 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_032461047 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_032462016 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_032462936 1 MDKK-YSIGLDIGINSVGWAVITDDYKVPSKKFKVLGNIDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_032464890 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGEIA--EATRLKRTARRRYT 73 WP_033888930 ---------------------------------------------------------------------------- WP_038431314 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_038432938 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_038434062 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 BAQ51233 ---------------------------------------------------------------------------- KGE60162 ---------------------------------------------------------------------------- KGE60856 ---------------------------------------------------------------------------- WP_002989955 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGEIA--EATRLKRTARRRYT 73 WP_003030002 1 MDQK-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 73 WP_003065552 1 MTKKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_001040076 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKIRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040078 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040080 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040081 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040083 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040085 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040087 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040088 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040089 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040090 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040091 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040092 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040094 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040095 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040096 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040097 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040098 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040099 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040100 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040104 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_001040105 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTSRRRYT 73 WP_001040106 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLEDGGNTA--SDRRLKRTARRRYT 73 WP_001040107 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLEDGGNTA--SDRRLKRTARRRYT 73 WP_001040108 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLEDGGNTA--SDRRLKRTARRRYT 73 WP_001040109 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLEDGGNTA--SDRRLKRTARRRYT 73 WP_001040110 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLEDGGNTA--SDRRLKRTARRRYT 73 WP_015058523 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_017643650 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_017647151 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--SDRRLKRTARRRYT 73 WP_017648376 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--SDRRLKRTARRRYT 73 WP_017649527 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_017771611 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--SDRRLKRTARRRYT 73 WP_017771984 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 CFQ25032 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 CFV16040 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 KLJ37842 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 KLJ72361 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 KLL20707 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 KLL42645 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--SDRRLKRTARRRYT 73 WP_047207273 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGRNTA--ADRRLKRTARRRYT 73 WP_047209694 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_050198062 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_050201642 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_050204027 1 MNKP-YSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--SDRRLKRTARRRYT 73 WP_050881965 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 WP_050886065 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 AHN30376 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 EAO78426 1 MNKP-YSIGXDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRTARRRYT 73 CCW42055 1 MNKP-YSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTA--ADRRLKRIARRRYT 73 WP_003041502 1 MNQK-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 73 WP_037593752 1 MKKE-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_049516684 1 MKKE-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 GAD46167 1 MKKE-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 73 WP_018363470 1 MTKKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_003043819 1 MEKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTNRKSIKKNLMGALLFDSGETA--EATRLKRTARRRYT 73 WP_006269658 1 MGKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 73 WP_048800889 1 MTQK-YSIGLDIGTNSVGWAIVTDDYKVPAKKMKILGNTNKQYIKKNLLGALLFDSGETA--KATRLKRTARRRYT 73 WP_012767106 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_014612333 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_015017095 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_015057649 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_048327215 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_049519324 1 MDKK-YSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_012515931 1 MKKP-YTIALDIGTNSVGWVVVTDDYRVPTKKMKVLGNTERKTIKKNLIGALLFDSGDTA--EGTRLKRTARRRYT 73 WP_021320964 1 MKKP-YTIALDIGTNSVGWVVVTDDYRVPTKKMKVLGNTERKTIKKNLIGALLFDSGDTA--EGTRLKRTARRRYT 73 WP_037581760 1 MKKP-YTIALDIGTNSVGWVVVTDDYRVPTKKMKVLGNTERKTIKKNLIGALLFDSGDTA--EGTRLKRTARPRYT 73 WP_004232481 1 M-EKtYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRAARRRYT 73 WP_009854540 1 MTKKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_012962174 1 MTEKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDNGETA--EATRLKRTARRRYT 74 WP_039695303 1 MTKKnYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EATRLKRTARRRYT 74 WP_014334983 1 M-EKsYSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTDKKYIKKNLLGALLFDSGETA--EVTRLKRTARRRYT 73 WP_003099269 1 MRKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMRIQGTTDRTSIKKNLIGALLFDNGETA--EATRLKRTTRRRYT 73 AHY15608 1 MRKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMRIQGTTDRTSIKKNLIGALLFDNGETA--EATRLKRTTRRRYT 73 AHY17476 1 MRKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMRIQGTTDRTSIKKNLIGALLFDNGETA--EATRLKRTTRRRYT 73 ESR09100 ---------------------------------------------------------------------------- AGM98575 1 MRKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMRIQGTTDRTSIKKNLIGALLFDNGETA--EATRLKRTTRRRYT 73 ALF27331 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_018372492 1 MKKP-YSIGLDIGTNSVGWAVVMEDYKVPSKKMKVLGNTDKQSIKKNLIGALLFDSGETAv--ERRLNRTTSRRYD 73 WP_045618028 1 NNKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMKVLGNTDKHFIKKNLLGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_045635197 1 K-KG-YSIGLDIGTNSVGFAVITDDYKVPSKKMKVLGNTDKRFIKKNLIGALLFDEGTTA--EARRLKRTARRRYT 73 WP_002263549 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002263887 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002264920 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002269043 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002269448 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002271977 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002272766 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002273241 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002275430 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_002276448 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002277050 1 MKKS-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002277364 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_002279025 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002279859 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002280230 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_002281696 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_002282247 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002282906 1 MKKP-YSIGLDIGTNSVGWSVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002283846 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002287255 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVSAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002288990 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002289641 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTTRRRYT 73 WP_002290427 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002295753 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002296423 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002304487 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002305844 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002307203 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002310390 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_002352408 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_012997688 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_014677909 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPDKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_019312892 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_019313659 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_019314093 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_019315370 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_019803776 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_019805234 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_024783594 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_024784288 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTARRRYT 73 WP_024784666 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_024784894 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTTRRRYT 73 WP_024786433 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_049473442 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 73 WP_049474547 1 MKKP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--EDRRLKRTTRRRYT 73 EMC03581 1 MDL--------IGTNSVGWAVVTDDYKVPAKKMKVLGNTDKSHIKKNLLGALLFDSGNTA--ADRRLKRTARRRYT 66 WP_000428612 1 ENKN-YSIGLDIGTNSVGWAVITDDYKVPSKKMKVLGNTDKRFIKKNLIGALLFDEGTTA--EARRLKRTARRRYT 74 WP_000428613 1 ENKN-YSIGLDIGTNSVGWSVITDDYKVPSKKMKVLGNTDKRFIKKNLIGALLFDEGTTA--EARRLKRTARRRYT 74 WP_049523028 1 K-KP-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTNKESIKKNLIGALLFDAGNTA--ADRRLKRTARRRYT 73 WP_003107102 1 --------------------------------MKVLGNTDRQTVKKNMIGTLLFDSGETA--EARRLKRTARRRYT 42 WP_054279288 1 -KKS-YSIGLDIGTNSVGWAVITDDYKVPAKKMKVLGNTSRQSIKKNMIGALLFDEGGPA--ASTRVKRTTRRRYT 75 WP_049531101 1 SNKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMKVLGNTDKHFIKKNLIGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_049538452 1 SNKP-YSIGLDIGTNSVGWVIITDDYKVPSKKMKVLGNTDKHFIKKNLIGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_049549711 1 SNKP-YSIGLDIGTNSVGWAVITDDYKVPSKKMTVLGNTDKHFIKKNLIGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_007896501 1 --YS-YSIGLDIGTNSVGWAVINEDYKVPAKKMTVFGNTDRKTIKKNLLGTVLFDSGETA--QARRLKRTNRRRYT 75 EFR44625 1 -----------------------------------------------MLGTVLFDSGETA--QARRLKRTNRRRYT 27 WP_002897477 1 K-KP-YSIGLDIGTNSVGWAVVTDDYKVPAKKMRVFGDTDRSHIKKNLLGTLLFDDGNTA--ESRRLKRTARRRYT 73 WP_002906454 1 K-KP-YSIGLDIGTNSVGWAVITDDYKVPSKKMKVLGNTDKHFIKKNLIGALLFDEGTTA--EDRRLKRTSRRRYT 73 WP_009729476 1 ENKN-YSIGLDIGTNSVGWSVITDDYKVPSKKMKVLGNTDKHFIKKNLIGALLFDEGTTA--EARRLKRTARRRYT 74 CQR24647 1 MKKP-YSIGLDIGTNSVGWSVVTDDYKVPAKKMKVLGNTDKEYIKKNLIGALLFDSGETA--EATRMKRTARRRYT 73 WP_000066813 1 SNKS-YSIGLDIGTNSVGWAVITDDYKVPSKKMKVLGNTDKHFIKKNLIGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_009754323 1 NNNN-YSIGLDIGTNSVGWAVITDDYKVPSKKMRVLGNTDKRFIKKNLIGALLFDEGTTA--EDRRLKRTARRRYT 74 WP_044674937 1 MKKK-YAIGIDIGTNSVGWSVVTDDYKVPSKKMKVFGNTEKRYIKKNLLGTLLFDEGNTA--ENRRLKRTARRRYT 73 WP_044676715 1 MKKK-YAIGIDIGTNSVGWSVVTDDYKVPSKKMKVFGNTEKRYIKKNLLGTLLFDEGNTA--ENRRLKRTARRRYT 73 WP_044680361 1 MKKK-YAIGIDIGTNSVGWSVVTDDYKVPSKKMKVFGNTEKRYIKKNLLGTLLFDEGNTA--ENRRLKRTARRRYT 73 WP_044681799 1 MKKK-YAIGIDIGTNSVGWSVVTDDYKVPSKKMKVFGNTEKRYIKKNLLGTLLFDEGNTA--ENRRLKRTARRRYT 73 WP_049533112 1 MDQK-YSIGLDIGTNSVGWAVVTDDYKVPAKKMKVLGNTDKQSIKKNLLGALLFDSGETA--EATRLKRTARRRYT 73 WP_029090905 1 -----------------------------------------------MWGVSLFEAGKTA--AERRGYRSTRRRLN 27 WP_006506696 1 I-VD-YCIGLDLGTGSVGWAVVDMNHRLMKRN------------GKHLWGSRLFSNAETA--ANRRASRSIRRRYN 60 AIT42264 1 MDKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_034440723 1 -MKN-YTIGLDIGTNSVGWAVIKDDLTLVRKKIKISGNTDKKEVKKNLWGSFLFEQGDTA--QDTRVKRIARRRYE 72 AKQ21048 1 MDKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 WP_004636532 1 MQKN-YTIGLDIGTNSVGWAVMKDDYTLIRKRMKVLGNTDIKKIKKNFWGVRLFDEGETA--KETRLKRGTRRRYQ 73 WP_002364836 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_016631044 1 --------------------------------------------------MRLFEEGHTA--EDRRLKRTARRRIS 24 EMS75795 ---------------------------------------------------------------------------- WP_002373311 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_002378009 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_002407324 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_002413717 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_010775580 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_010818269 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_010824395 1 MKKD-YVIGLDIGSNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_016622645 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_033624816 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_033625576 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_033789179 1 MKKD-YVIGLDIGTNSVGWAVMTEDYQLVKKKMPIYGNTEKKKIKKNFWGVRLFEEGHTA--EDRRLKRTARRRIS 73 WP_002310644 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002312694 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002314015 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002320716 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002330729 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002335161 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_002345439 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_034867970 1 MTKD-YTIGLDIGTNSVGWAVLTDDYQLMKRKMSVHGNTEKKKIKKNFWGARLFDEGQTA--EFRRTKRTNRRRLA 73 WP_047937432 1 MKKE-YTIGLDIGTNSVGWSVLTDDYRLVSKKMKVAGNTEKSSTKKNFWGVRLFDEGQTA--EARRSKRTARRRLA 73 WP_010720994 1 MTKD-YTIGLDIGTNSVGWAVLTDDYQLMKRKMSVHGNTEKKKIKKNFWGARLFDEGQTA--EFRRTKRTNRRRLA 73 WP_010737004 1 MTKD-YTIGLDIGTNSVGWAVLTDDYQLMKRKMSVHGNTEKKKIKKNFWGARLFDEGQTA--EFRRTKRTNRRRLA 73 WP_034700478 1 MTKD-YTIGLDIGTNSVGWAVLTDDYQLMKRKMSVHGNTEKKKIKKNFWGARLFDEGQTA--EFRRTKRTNRRRLA 73 WP_007209003 1 MKND-YTIGLDIGTNSVGYSVVTDDYKVISKKMNVFGNTEKKSIKKNFWGVRLFESGQTA--QEARMKRTSRRRIA 73 WP_023519017 1 MEKE-YTIGLDIGTNSVGWAVLTDDYRLVARKMSIQGDSNRKKIKKNFWGARLFEEGKTA--QFRRIKRTNRRRIA 73 WP_010770040 1 MKKE-YTIGLDIGTNSVGWAVLTENYDLVKKKMKVYGNTETKYLKKNLWGVRLFDEGETA--ADRRLKRTTRRRYS 73 WP_048604708 1 MGKE-YTIGLDIGTNSVGWAVLQEDLDLVRRKMKVYGNTEKNYLKKNFWGVDLFDEGMTA--KDTRLKRTTRRRYF 73 WP_010750235 1 MNKA-YTLGLDIGTNSVGWAVVTDDYRLMAKKMPVHSKMEKKKIKKNFWGARLFDEGQTA--EERRNKRATRRRLR 73 AII16583 1 ADKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 112 WP_029073316 1 NNKI-YNIGLDIGDASVGWAVVDEHYNLLKRH------------GKHMWGSRLFTQANTA--VERRSSRSTRRRYN 65 WP_031589969 1 NNKI-YNIGLDIGDASVGWAVVDEHYNLLKRH------------GKHMWGSRLFTQANTA--VERRSSRSTRRRYN 65 KDA45870 1 LKKD-YSIGLDIGTNSVGHAVVTDDYKVPTKKMKVFGDTSKKTIKKNMLGVLLFNEGQTA--ADTRLKRGARRRYT 74 WP_039099354 1 MSRP-YNIGLDIGTSSIGWSVVDDQSKLVSVR------------GKYGYGVRLYDEGQTA--AERRSFRTTRRRLK 61 AKP02966 1 KEQP-YNIGLDIGTGSVGWAVTNDNYDLLNIK------------KKNLWGVRLFEGAQTA--KETRLNRSTRRRYR 64 WP_010991369 1 MKKP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKIAGDSEKKQIKKNFWGVRLFDEGQTA--ADRRMARTARRRIE 73 WP_033838504 1 MKKP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKIAGDSEKKQIKKNFWGVRLFDEGQTA--ADRRMARTARRRIE 73 EHN60060 1 MKKP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKIAGDSEKKQIKKNFWGVRLFDEGQTA--ADRRMARTARRRIE 76 EFR89594 ---------------------------------------------------------------------------- WP_038409211 1 MRKP-YTIGLDIGTNSVGWAVLTDQYNLVKRKMKVAGSAEKKQIKKNFWGVRLFDEGEVA--AGRRMNRTTRRRIE 73 EFR95520 ---------------------------------------------------------------------------- WP_003723650 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_003727705 1 MKNP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_003730785 1 MKNP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_003733029 1 MKKP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKISGDSEKKQIKKNFWGVRLFEKGETA--AKRRMSRTARRRIE 73 WP_003739838 1 MKNP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDEGETA--ADRRMNRTARRRIE 73 WP_014601172 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_023548323 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_031665337 1 MKNP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 WP_031669209 1 MKKP-YTIGLDIGTNSVGWAVLTDQYDLVKRKMKISGDSEKKQIKKNFWGVRLFEKGETA--AKRRMSRTARRRIE 73 WP_033920898 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 73 AKI42028 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 76 AKI50529 1 MKNP-YTIGLDIGTNSVGWAVLTNQYDLVKRKMKVAGNSDKKQIKKNFWGVRLFDDGQTA--VDRRMNRTARRRIE 76 EFR83390 ---------------------------------------------------------------------------- WP_046323366 1 MKKP-YTIGLDIGTNSVGWAALTDQYDLVKRKMKVAGNSEKKQIKKNLWGVRLVDEGKTA--AHRRVNRTTRRRIE 73 AKE81011 1 ADKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 89 CUO82355 1 I-VD-YCIGLDLGTGSVGWAVVDMNHRLMKRN------------GKHLWGSRLFSNAETA--ATRRSSRSIRRRYN 64 WP_033162887 1 KDIR-YSIGLDIGTNSVGWAVMDEHYELLKKG------------NHHMWGSRLFDAAEPA--ATRRASRSIRRRYN 65 AGZ01981 1 ADKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 106 AKA60242 1 MDKK-YSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 AKS40380 1 MDKK-YSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 73 4UN5_B 1 MDKK-YSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA--EATRLKRTARRRYT 77 WP_010922251 74

143 WP_039695303 75 RRKNRLRYLQEIFANEIAKVDESFFQRLDE-SFLT--DDDKT---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 144 WP_045635197 74 RRKNRLRYLQEIFSEEMSKVDSSFFHRLDD-SFLI--PEDKR---E SKYPIFATLT-EEKEYHKQFPTIYHLRKQLA 143 5AXW_A 62 RRRHRIQRVKKLLFD---------YNLLTDhSELS----------G --NPYEARVK--------------GLSQKLS 104 WP_009880683 ---------------------------------------------- ------------------------------- WP_010922251 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_011054416 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_011284745 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_011285506 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_011527619 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_012560673 74 RRKNRICYLQEIFSNEIAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_014407541 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_020905136 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_023080005 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_023610282 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_030125963 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_030126706 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_031488318 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_032460140 74 RRKNRICYLQEIFSNEIAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_032461047 74 RRKNRICYLQEIFSNEIAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_032462016 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_032462936 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_032464890 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_033888930 ---------------------------------------------- ------------------------------- WP_038431314 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_038432938 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_038434062 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 BAQ51233 1 ----------------MAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 54 KGE60162 ---------------------------------------------- ------------------------------- KGE60856 ---------------------------------------------- ------------------------------- WP_002989955 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_003030002 74 RRRNRLRYLQEIFAEEMNKVDENFFQRLDD-SFLV--DEDKR---G ERHPIFGNIA-AEVKYHDDFPTIYHLRKHLA 143 WP_003065552 75 RRKNRLRYLQEIFAEEMTKVDESFFQRLDE-SFLRwdDDNKK---L GRYPIEGNKA-DVVKYHQEEPTIYHLRKHLA 146 WP_001040076 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040078 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040080 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040081 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040083 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040085 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040087 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040088 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040089 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040090 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040091 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040092 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040094 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040095 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040096 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040097 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040098 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040099 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040100 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_001040104 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYXIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040105 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_001040106 74 CRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040107 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040108 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040109 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_001040110 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_015058523 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_017643650 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_017647151 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_017648376 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_017649527 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFPTIYHLRKELA 143 WP_017771611 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_017771984 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 CFQ25032 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 CFV16040 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 KLJ37842 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 KLJ72361 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 KLL20707 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 KLL42645 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_047207273 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_047209694 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKYYHEKFPTIYHLRKELA 143 WP_050198062 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_050201642 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_050204027 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EDDKR---G SKYPIFATMQ-EEKDYHEKFPTIYHLRKELA 143 WP_050881965 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 WP_050886065 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 AHN30376 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATMQ-EEKDYHEKEPTIYHLRKELA 143 EAO78426 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKFSTIYHLRKELA 143 CCW42055 74 RRRNRILYLQEIFAEKMSKVDDSFFHRLED-SFLV--EEDKR---G SKYPIFATLQ-EEKDYHEKEPTIYHLRKELA 143 WP_003041502 74 RRRNRLRYLQEIFAEEMMQVDESFFQRLDD-SFLV--DEDKR---G ERHPIEGNIA-AEVKYHDEEPTIYHLRKHLA 143 WP_037593752 75 RRKNRLRYLQEIFTEEMNKVDENFFQRLDD-SFLV--EEDKQ---G SKYPIEGILK-EEKEYHKKEKTIYHLREELA 144 WP_049516684 75 RRRNRLRYLQEIFAEEMMQVDESFFQRLDD-SFLV--EEDKR---G SRYPIEGNIA-AEVKYHDDEPTIYHLRKHLV 144 GAD46167 74 RRKNRLRYLQEIFTEEMNKVDENFFQRLDD-SFLV--EEDKQ---G SKYPIEGILK-EEKEYHKKEKTIYHLREELA 143 WP_018363470 75 RRKNRLRYLQDIFTEEMAKVDDSFFQRLDE-SELT--DNDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 144 WP_003043819 74 RRKNRIRYLQEIFANEMAKLDDSFFQRLEE-SFLV--EEDKK---N ERHPIFGNLA-DEVAYHRNYPTIYHLRKKLA 143 WP_006269658 74 RRKNRLRYLQEIFTGEMNKVDENFFQRLDD-SFLV--DEDKR---G EHHPIEGNIA-AEVKYHDDEPTIYHLRRHLA 143 WP_048800889 74 RRKNRLRYLQEIFIEEMNKVDENFFQRLDD-SFLV--TEDKR---G SKYPIEGILK-EEKEYYKEFETIYHLRKRLA 143 WP_012767106 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_014612333 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_015017095 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_015057649 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_048327215 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_049519324 74 RRKNRIRYLQEIFSSEMSKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLA 143 WP_012515931 74 RRKNRLRYLKEIFTEEMAKVDDGFFQRLED-SFYV--LEDKE---G NKHPIFANLA-DEVAYHKKYPTIYHLRKELV 143 WP_021320964 74 RRKNRLRYLKEIFTEEMAKVDDGFFQRLED-SFYV--LEDKE---G NKHPIFANLA-DEVAYHKKYPTIYHLRKELV 143 WP_037581760 74 RRKNRLRFLKEIFTEEMAKVDDGFFQRLED-SFYV--LEDKE---G NKHPIFANLA-DEVAYHKKYPTIYHLRKELV 143 WP_004232481 74 RRKNRLRYLQEIFAKEMAKVDESFFQRLEE-SELT--DDDKT---F DSHPIEGNKA-EEDTYHQEFPTIYHLRKHLA 143 WP_009854540 75 RRKNRLRYLQEIFAEEMTKVDESFFYRLDE-SELT--TDEKD---F ERHPIEGNKA-EEDAYHQKEPTIYHLRNYLA 144 WP_012962174 75 RRKNRLRYLQEIFAEEMAKVDESFFYRLDE-SELT--TDDKD---F ERHPIFGNKA-DEIKYHQEFPTIYHLRKHLA 144 WP_039695303 75 RRKNRLRYLQEIFANEIAKVDESFFQRLDE-SELT--DDDKT---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 144 WP_014334983 74 RRKNRLRYLQEIFAKEMTKVDESFFQRLEE-SELT--DDDKT---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKYLA 143 WP_003099269 74 RRKYRIKELQKIFSSEMNELDIAFFPRLSE-SFLV--SDDKE---F ENHPIFGNLK-DEITYHNDYPTIYHLRQTLA 143 AHY15608 74 RRKYRIKELQKIFSSEMNELDIAFFPRLSE-SFLV--SDDKE---F ENHPIFGNLK-DEITYHNDYPTIYHLRQTLA 143 AHY17476 74 RRKYRIKELQKIFSSEMNELDIAFFPRLSE-SFLV--SDDKE---F ENHPIFGNLK-DEITYHNDYPTIYHLRQTLA 143 ESR09100 ---------------------------------------------- ------------------------------- AGM98575 74 RRKYRIKELQKIFSSEMNELDIAFFPRLSE-SFLV--SDDKE---F ENHPIFGNLK-DEITYHNDYPTIYHLRQTLA 143 ALF27331 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLED-SFLV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_018372492 74 RRRNRIRYLQHIFAEEMNRADENFFHRLKE-SFFV--EEDKT---Y SKYPIFGTLE-EEKNYHKNYPTIYHLRKTLA 143 WP_045618028 75 RRKNRLRYLQEIFTEEMSKVDISFFHRLDD-SFLV--PEDKR---G SKYPIFATLE-EEKEYHKNFPTIYHLRKHLA 144 WP_045635197 74 RRKNRLRYLQEIFSEEMSKVDSSFEHRLDD-SFLI--PEDKR---E SKYPIFATLT-EEKEYHKQFPTIYHLRKQLA 143 WP_002263549 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLED-SFLV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002263887 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLED-SFLV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002264920 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLDE-SELT--DDDKN---F DSYPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_002269043 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLED-SFLV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002269448 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002271977 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002272766 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002273241 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002275430 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002276448 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002277050 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLDE-SFLT--DDDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_002277364 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002279025 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-FELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002279859 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLDE-SFLT--DDDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_002280230 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002281696 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002282247 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLDE-SFLT--DDDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_002282906 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002283846 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002287255 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002288990 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002289641 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002290427 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002295753 74 RRRNRILYLQEIFSEEMGKVNDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002296423 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002304487 74 RRRNRILYLQEIFAEEMMQVDESFFQRLDD-SFLV--EEDKR---G SRYPIEGILK-EEKKYHKEEKTIYHLREKLA 143 WP_002305844 74 RRRNRILYLQEIFSEEMDKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002307203 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002310390 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_002352408 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_012997688 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_014677909 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019312892 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019313659 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019314093 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019315370 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ECHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019803776 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_019805234 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_024783594 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_024784288 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLDE-SFLT--DDDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_024784666 74 RRRNRILYLQEIFAEEMSKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_024784894 74 RRRNRILYLQEIFSEEMGKVDDSFEHRLED-SELV--TEDKR---G ERHPIEGNLE-EEVKYHENEPTIYHLRQYLA 143 WP_024786433 74 RRRNRILYLQEIFAEEMNKVDDSFEHRLDE-SFLT--DDDKN---F DSHPIEGNKA-EEDAYHQKEPTIYHLRKHLA 143 WP_049473442 74 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--TEDKR---G ERHPIFGNLE-EEVKYYENFPTIYHLRQYLA 143 WP_049474547 74 RRRNRILYLQEIFSEEMGKVDDSFFHRLED-SFLV--TEDKR---G ERHPIFGNLE-EEVKYHENFPTIYHLRQYLA 143 EMC03581 67 RRRNRILYLQEIFAEEMSKVDDSFFHRLED-SFLV--TEDKR---G ERHPIFGNLE-EEVKYHENFPTIYHLRQYLA 136 WP_000428612 75 RRKNRLRYLQEIFAEEMSKVDSSFFHRLDD-SFLI--PEDKK---G SKYPIFATLI-EEKEYHKQFPTIYHLRKQLA 144 WP_000428613 75 RRKNRLRYLQEIFAEEMSKVDSSFFHRLDD-SFLI--PEDKR---G SKYPIFATLA-EEKEYHKQFPTIYHLRKQLA 144 WP_049523028 74 RRRNRILYLQEIFAAEMNKVDESFFHRLDD-SFLV--PEDKR---G SKYPIFGTLE-EEKEYHKQFPTIYYLRKILA 143 WP_003107102 43 RRINRIKYLQSIFDDEMSKIDSAFFQRIKD-SFLV--PDDKN---D DRHPIFGNIK-DEVDYHKNYPTIYHLRKKLA 112 WP_054279288 76 RRKNRLCYLRDIFESEMHTIDKHFFLRLED-SFLH--KSDKR---Y EAHPIEGTLQ-EEKAYHDNYPTIYHLRKALA 145 WP_049531101 75 RRKNRLRYLQEIFSEEISKVDNSFFHRLDD-SFLV--PEDKR---G SKYPIFATLT-EEKEYYKQFPTIYHLRKQLA 144 WP_049538452 75 RRKNRLRYLQEIFAEEMNKVDSSFFHRLDD-SFLV--PEDKR---G SKYPIFATLA-EEKEYHKNFPTIYHLRKQLA 144 WP_049549711 75 RRKNRLRYLQEIFSGEMSKVDSSFFHRLDD-SFLV--PEDKR---G SKYPIFATLV-EEKEYHKQFPTIYHLRKQLA 144 WP_007896501 76 RRRYRLCQLQNIFATEMVKVDDIFFQRLSE-SFFY--YQDKA---F DKHPIFGNSK-EERAYHKTYPTIYHLRKDLA 145 EFR44625 28 RRRYRLCQLQNIFATEMVKVDDIFFQRLSE-SFFY--YQDKA---F DKHPIFGNSK-EERAYHKTYPTIYHLRKDLA 97 WP_002897477 74 RRRNRILYLQEIFTESMNEIDESFFHRLDD-SFLV--PEDKR---G SKYPIFATLQ-EEKEYHKQFPTIYHLRKQLA 143 WP_002906454 74 RRKNRLRYLQEIFSEEISKLDSSFFHRLDD-SFLV--PEDKR---G SKYPIFATLE-EEKEYHKKFPTIYHLRKHLA 143 WP_009729476 75 RRKNRLRYLQEIFSEEIGKVDSSFFHRLDD-SFLI--PEDKR---G SKYPIFATLA-EEKKYHKQFPTIYHLRKQLA 144 CQR24647 74 RRRNRILYLQDIFSPELNQVDESFLHRLDD-SFLVa--EDKR---G ERHVIFGNIA-DEVKYHKEFPTIYHLRKHLA 143 WP_000066813 75 RRKNRLRYLQEIFSQEISKVDSSFFHRLDD-FFLV--PEDKR---G SKYPIFATLV-EEKEYHKKEPTIYHLRKHLA 144 WP_009754323 75 RRKNRLRYLQEIFAEEMSKVDSSFFHRLDD-SFLV--PEDKS---G SKYPIFATLA-EEKEYHKKFPTIYHLRKHLA 144 WP_044674937 74 RRRNRILYLQEIFAEEINKIDDSFFQRLDD-SFLIv--EDKQ---G SKHPIFGTLQ-EEKKYHKQFPTIYHLRKQLA 143 WP_044676715 74 RRRNRILYLQEIFAEEINKIDDSFFQRLDD-SFLIv--EDKQ---G SKHPIFGTLQ-EEKEYHKQFPTIYHLRKQLA 143 WP_044680361 74 RRRNRILYLQEIFAEEINKIDDSFFQRLDD-SFLIv--EDKQ---G SKHPIFGTLQ-EEKEYHKQFPTIYHLRKQLA 143 WP_044681799 74 RRRNRILYLQEIFAEEINKIDDSFFQRLDD-SFLIv--EDKQ---G SKHPIFGTLQ-EEKKYHKQFPTIYHLRKQLA 143 WP_049533112 74 RRRNRLRYLQEIFAEEMNKVDENFFQRLDD-SFLV--DEDKR---G ERHPIFGNIA-AEVKYHDDFPTIYHLRKHLA 143 WP_029090905 28 HRKFRLRLLEDMFEKEILSKDPSFFIRLKE-AFLSpkDEQKQ---F ----LFNDKDyTDADYYEQYKTIYHLRYDLI 100 WP_006506696 61 KRRERIRLLRAILQDMVLEKDPTFFIRLEHtSFLD--EEDKAkylG DNYNLFIDEDfNDYTYYHKYPTIYHLRKALC 139 AIT42264 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_034440723 73 RRRFRIRELQKIFDKSMGEVDSNFFHRLDE-SFLV--EEDKE---Y SKYPIFSNEK-EDKNYYDKYPTIYHLRKDLA 142 AKQ21048 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 WP_004636532 74 RRRNRLIYLQDIFQQPMLAIDENFFHRLDD-SFFV--PDDKS---Y DRHPIFGSLE-EEVAYHNTYPTIYHLRKHLA 143 WP_002364836 74 RRRNRLRYLQAFFEEAMIDLDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_016631044 25 RRRNRLRYLQAFFEEAMIDLDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 94 EMS75795 ---------------------------------------------- ------------------------------- WP_002373311 74 RRRNRLRYLQAFFEEAMIDLDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_002378009 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_002407324 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_002413717 74 RRRNRLRYLQAFFEEAMIDLDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_010775580 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_010818269 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_010824395 74 RRRNRLRYLQAFFEEAMTDLDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_016622645 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_033624816 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_033625576 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_033789179 74 RRRNRLRYLQAFFEEAMTALDENFFARLQE-SFLV--PEDKK---W HRHPIFAKLE-DEVAYHETYPTIYHLRKKLA 143 WP_002310644 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002312694 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--PDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002314015 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002320716 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002330729 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002335161 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_002345439 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_034867970 74 RRKYRLSKLQDLFAEELCKQDDCFFVRLEE-SFLV--PEEKQ---Y KPASIFPTLE-EEKEYYQKYPTIYHLRQKLV 143 WP_047937432 74 RRRQRILELQKIFAPEILKIDEHFFARLNE-SFLV--LDEKK---Q SRHPVFATIK-QEKSYHQTYPTIYHLRQALA 143 WP_010720994 74 RRKYRLSKLQDLFAEELCKQDDCFFVRLEE-SFLV--PEEKQ---Y KPASIFPTLE-EEKEYYQKYPTIYHLRQKLV 143 WP_010737004 74 RRKYRLSKLQDLFAEELCKQDDCFFVRLEE-SFLV--PEEKQ---Y KPASIFPTLE-EEKEYYQKYPTIYHLRQKLV 143 WP_034700478 74 RRKYRLSKLQDLFAEELCKQDDCFFVRLEE-SFLV--PEEKQ---Y KPASIFPTLE-EEKEYYQKYPTIYHLRQKLV 143 WP_007209003 74 RRKNRICYLQEIFQPEMNHLDNNFFYRLNE-SFLVa--DDAK---Y DKHPIFGILD-EEIHFHEQFPTIYHLRKYLA 143 WP_023519017 74 RRRQRVLALQDIFAEEIHKKDPNFFARLEE-GDRV--EADKR---F AKFFVFAILS-EEKNYHRQYPTIYHLRHDLA 143 WP_010770040 74 RRRNRICRLQDLFTEEMNQVDANFFHRLQE-SFLV--PDEKE---F ERHAIFGKME-EEVSYYREFPTIYHLRKHLA 143 WP_048604708 74 RRRQRISYLQIFFQEEMNRIDPNFFNRLDE-SFLI--EEDKL---S ERHPIFGTIE-EEVAYHKNYATIYHLRKELA 143 WP_010750235 74 RRKYRILELQKIFSEEILKKDSHFFARLDE-SFLI--PEDKQ---Y ARFPIFPILL-EEKAYYQNYPTIYHLRQKLA 143 AII16583 113 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 182 WP_029073316 66 KRRERIRLLRGIMEDMVLDVDPIFFIRLANvSFLD--QEDKKdylK SNYNLFIDKDfNDKTYYDKYPTIYHLRKHLC 144 WP_031589969 66 KRRERIRLLREIMEDMVLDVDPIFFIRLANvSFLD--QEDKKdylK SNYNLFIDKDfNDKTYYDKYPTIYHLRKHLC 144 KDA45870 75 RRKNRLRYLQEIFAPALAKVDPNFFYRLEE-SSLVa--EDKK---Y DVYPIFGKRE-EELLYHDTHKTIYHLRSELA 144 WP_039099354 62 RRKWRLGLLREIFEPYITPVDDIFFLRKKQ-SNLS--PKDQR---K -QTSLENDRT--DRAFYDDYPTIYHLRYKLM 132 AKP02966 65 RRKNRINWLNEIFSEELANTDPSFLIRLQN-SWVSkkDPDRK---R DKYNLFIDNPyTDKEYYREFPTIFHLRKELI 137 WP_010991369 74 RRRNRISYLQGIFAEEMSKTDANFFCRLSD-SFYV--DNEKR---N SRHPFFATIE-EEVEYHKNYPTIYHLREELV 143 WP_033838504 74 RRRNRISYLQGIFAEEMSKTDANFFCRLSD-SFYV--DNEKR---N SRHPFFATIE-EEVEYHKNYPTIYHLREELV 143 EHN60060 77 RRRNRISYLQGIFAEEMSKTDANFFCRLSD-SFYV--DNEKR---N SRHPFFATIE-EEVEYHKNYPTIYHLREELV 146 EFR89594 ---------------------------------------------- ------------------------------- WP_038409211 74 RRRNRIAYLQEIFAAEMAEVDANFFYRLED-SFYI--ESEKR---H SRHPFFATIE-EEVAYHEEYKTIYHLREKLV 143 EFR95520 ---------------------------------------------- ------------------------------- WP_003723650 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFFATIE-EEVAYHDNYRTIYHLREKLV 143 WP_003727705 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFFATIE-EEVAYHKNYRTIYHLREELV 143 WP_003730785 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFFATIE-EEVAYHKNYRTIYHLREELV 143 WP_003733029 74 RRRNRISYLQEIFAIQMNEVDDNFFNRLKE-SFYA--ESDKK---Y NRHPFFGTVE-EEVAYYKDFPTIYHLRKELI 143 WP_003739838 74 RRRNRISYLQEIFALEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFFATIE-EEVAYHKNYRTIYHLREELV 143 WP_014601172 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 143 WP_023548323 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 143 WP_031665337 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 143 WP_031669209 74 RRRNRISYLQEIFAIQMNEVDDNFFNRLKE-SFYA--ESDKK---Y NRHPFEGTVE-EEVAYYKDEPTIYHLRKELI 143 WP_033920898 74 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 143 AKI42028 77 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 146 AKI50529 77 RRRNRISYLQEIFAVEMANIDANFFCRLND-SFYV--DSEKR---N SRHPFEATIE-EEVAYHKNYRTIYHLREELV 146 EFR83390 ---------------------------------------------- ------------------------------- WP_046323366 74 RRRNRISYLQEIFTAEMFEVDANFFYRLED-SFYI--ESEKR---Q SRHPFEATIE-EEVAYHENYRTIYHLREKLV 143 AKE81011 90 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 159 CUO82355 65 KRRERIRLLRAILQDMVLEKDPTFFIRLEHtSFLD--EEDKAkylG DNYNLFIDEDfNDYTYYHKYPTIYHLRKALC 143 WP_033162887 66 KRRERIRLLRDLLGDMVMEVDPIFFIRLLNvSFLD--EEDKQknlG DNYNLFIEKDfNDKTYYDKYPTIYHLRKELC 144 AGZ01981 107 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 176 AKA60242 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 AKS40380 74 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 143 4UN5_B 78 RRKNRICYLQEIFSNEMAKVDDSFFHRLEE-SFLV--EEDKK---H ERHPIFGNIV-DEVAYHEKYPTIYHLRKKLV 147 WP_010922251 144

211 WP_039695303 145 DSSEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKI--FADFVGVYNRT--FDDS-H LSEITVDVA---SI 212 WP_045635197 144 DSKEKTDLRLIYLALAHMIKYRGHFLYEEA-FDIKNNDIQKI--FNEFISIYDNT--FEGS-S LSGQNAQVE---AI 211 5AXW_A 105 EEEFSA-------ALLHLAKRRG---VHNV------NEVE------------EDT----GN-- --------E----- 134 WP_009880683 --------------------------------------------------------------- -------------- WP_010922251 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_011054416 144 DSTDKVDLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_011284745 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_011285506 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_011527619 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_012560673 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_014407541 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQIYNQL--FEEN-- INASRVDAK---AI 211 WP_020905136 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_023080005 144 DSTDKVDLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_023610282 144 DSTDKVDLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_030125963 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_030126706 144 DSTDKVDLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_031488318 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_032460140 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_032461047 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGG-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_032462016 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INANGVDAK---AI 211 WP_032462936 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_032464890 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_033888930 1 ---------------------------------PDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 36 WP_038431314 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_038432938 144 DSTDKVDLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_038434062 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 BAQ51233 55 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 122 KGE60162 --------------------------------------------------------------- -------------- KGE60856 --------------------------------------------------------------- -------------- WP_002989955 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_003030002 144 DISQKADLRLVYLALAHMIKFRGHFLIEGQ-LKAENTNVQAL--FKDFVEVYDKT--VEES-H LSEMTVDAL---SI 211 WP_003065552 147 DSSEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKI--FADFVGVYDRT--FDDS-H LSEITVDAA---SI 214 WP_001040076 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQDVDVE---AI 212 WP_001040078 144 DKQEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-H LLSQNVDVE---AI 212 WP_001040080 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040081 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNTI--FENN-D LLSQNVDVE---AI 212 WP_001040083 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040085 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040087 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040088 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040089 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040090 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040091 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040092 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTS--FENN-H LLSQNVDVE---AI 212 WP_001040094 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040095 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040096 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040097 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040098 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040099 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040100 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_001040104 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040105 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_001040106 144 DKKEKANLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_001040107 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_001040108 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_001040109 144 DKKEKANLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_001040110 144 DKKEKANLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_015058523 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTS--FENN-H LLSQNVDVE---AI 212 WP_017643650 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDrFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_017647151 144 DKKEKADLRLFYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDIE---GI 212 WP_017648376 144 DKKEKADLRLFYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_017649527 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_017771611 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_017771984 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 CFQ25032 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 CFV16040 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 KLJ37842 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 KLJ72361 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 KLL20707 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 KLL42645 144 DKKEKANLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_047207273 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_047209694 144 DKKEKADLRLIYLALAHIIKFRGHFLIEDDsFDVRNIDIQKQ--YQAFLEIFDTT--FENN-D LLSQNVDVE---AI 212 WP_050198062 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_050201642 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_050204027 144 DKKEKANLRLVYLALAHIIKFRGHFLIEDDsFDVRNIDIQRQ--YQAFLEIFDTT--FENN-H LLSQNIDVE---GI 212 WP_050881965 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNIDISKQ--YQDFLEIFNIT--FENN-D LLSQNVDVE---AI 212 WP_050886065 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNTDISKQ--YQDFLEIENTT--FENN-D LLSQNVDVE---AI 212 AHN30376 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDrEDVRNTDIQKQ--YQAFLEIEDTS--FENN-H LLSQNVDVE---AI 212 EAO78426 144 DKKEKADLRLIYIALAHIIKFRGHFLIEDDsFDVRNTDISKQ--YQDFLEIENTT--FENN-D LLSQNVDVE---AI 212 CCW42055 144 DKKEKADLRLVYLALAHIIKFRGHFLIEDDrEDVRNTDIQKQ--YQAFLEIFDTT--FENN-H LLSQNVDVE---AI 212 WP_003041502 144 DISQKADLRLVYLALAHMIKFRGHFLIEGQ-LKAENTNVQAL--FKDFVEVYDKT--VEES-H LSEITVDAL---SI 211 WP_037593752 145 NSKEKADLRLVYLALAHMIKFRGHFLYEGD-LKAENTDVQAL--FKDFVEEYDKT--IEES-H LSEITVDAL---SI 212 WP_049516684 145 DISQKADLRLVYLALAHMIKFRGHFLIEGQ-LKAENTNVQAL--FKDFVEVYDKT--VEES-H LSEMTVDAL---SI 212 GAD46167 144 NSKEKADLRLVYLALAHMIKFRGHFLYEGD-LKAENTDVQAL--FKDFVEEYDKT--IEES-H LSEITVDAL---SI 211 WP_018363470 145 DSTEKADLRLVYLALAHMIKERGHFLIEGE-LNAENTDVQKL--FTDEVGVYDRT--FDDS-H LSEITVDAA---SI 212 WP_003043819 144 DSPEKADLRLIYLALAHIIKFRGHFLIEGK-LNAENSDVAKL--FYQLIQTYNQL--FEES-- LDEIEVDAK---GI 211 WP_006269658 144 DTSKKADLRLVYLALAHMIKFRGHFLYEGD-LKAENTDVQAL--FKDFVEEYDKT--IEES-H LSEITVDAL---SI 211 WP_048800889 144 DSTGKVDLRLVYLALAHMIKFRGHFLIEGQ-LKAENTDVQTL--ENDEVEVYDKT--IEES-H LAEITVDAL---SI 211 WP_012767106 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDMDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_014612333 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEK-- INASGVDAK---AI 211 WP_015017095 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDMDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_015057649 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDMDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_048327215 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDMDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_049519324 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASRVDAK---AI 211 WP_012515931 144 DNPQKADLRLIYLAVAHIIKFRGHFLIEGT-LSSKNNNLQKS--FDHLVDTYNLL--FEEQ-- LLTEGINAK---EL 211 WP_021320964 144 DNPQKADLRLIYLAVAHIIKFRGHFLIEGT-LSSKNNNLQKS--FDHLVDTYNLL--FEEQ-- LLTEGINAK---EL 211 WP_037581760 144 DNPQKADLRLIYLAVAHIIKFRGHFLIEGT-LSSKNNNLQKS--FDHLVDTYNLL--FEEQ-- LLTEGINAK---EL 211 WP_004232481 144 DSPEKVDLRLVYLALAHMIKFRGHFLIEGQ-LNAENTDVQKI--FADFVGVYDRT--FDDS-H LSEITVDAA---SI 211 WP_009854540 145 DSSEKADLRLVYLALAHMIKYRGHFLIEGK-LNAENTDVQKL--FTDEVGVYDRT--FDDS-H LSEITVDVA---ST 212 WP_012962174 145 DSHEKADLRLIYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FEAFVEVYDRT--FDDS-N LSEITVDAS---SI 212 WP_039695303 145 DSSEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKI--FADFVGVYNRT--FDDS-H LSEITVDVA---SI 212 WP_014334983 144 DSQEKADLRLVYLALAHMIKYRGHFLIEGE-LNAENTDVQKL--ENVEVETYDKI--VDES-H LSEIEVDAS---SI 211 WP_003099269 144 DSDQKADLRLIYLALAHIIKFRGHFLIEGN-LDSENTDVHVL--FLNLVNIYNNL--FEED-- VETASIDAE---KI 211 AHY15608 144 DSDQKADLRLIYLALAHIIKFRGHFLIEGN-LDSENTDVHVL--FLNLVNIYNNL--FEED-- VETASIDAE---KI 211 AHY17476 144 DSDQKADLRLIYLALAHIIKFRGHFLIEGN-LDSENTDVHVL--FLNLVNIYNNL--FEED-- VETASIDAE---KI 211 ESR09100 --------------------------------------------------------------- -------------- AGM98575 144 DSDQKADLRLIYLALAHIIKFRGHFLIEGN-LDSENTDVHVL--FLNLVNIYNNL--FEED-- VETASIDAE---KI 211 ALF27331 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_018372492 144 DTPDKMDIRLIYLALAHIIKYRGHFLIEGD-LDIENIGIQDS--FKSFIEEYNTQ--FGTK-- -LDSTTKVE---AI 209 WP_045618028 145 DSKEKADFRLIYLALAHIIKYRGHFLYEES-FDIKNNDIQKI--FNEFISIYDNT--EEGS-S LNGQNAQVE---AI 212 WP_045635197 144 DSKEKTDLRLIYLALAHMIKYRGHFLYEEA-FDIKNNDIQKI--FNEFISIYDNT--EEGS-S LSGQNAQVE---AI 211 WP_002263549 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002263887 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002264920 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_002269043 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002269448 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002271977 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002272766 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002273241 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002275430 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002276448 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002277050 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_002277364 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002279025 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002279859 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_002280230 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002281696 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002282247 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_002282906 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002283846 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002287255 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002288990 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002289641 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002290427 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002295753 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002296423 144 DNPEKTDLRLVYLALAHIIKFGGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002304487 144 NSTEKADLRLVYLSLAHMIKFRGHFLIEGQ-LKAENTNVQAL--FKDFVEVYDKT--VEES-H LSEMTVDAL---SI 211 WP_002305844 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002307203 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQKL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002310390 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_002352408 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_012997688 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_014677909 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019312892 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019313659 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019314093 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQKL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019315370 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019803776 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_019805234 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_024783594 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_024784288 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_024784666 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_024784894 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_024786433 144 DSTEKADLRLVYLALAHMIKFRGHFLIEGE-LNAENTDVQKL--FADFVGVYDRT--FDDS-H LSEITVDAS---SI 211 WP_049473442 144 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 WP_049474547 144 DNPEKTDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 211 EMC03581 137 DNPEKVDLRLVYLALAHIIKFRGHFLIEGK-FDTRNNDVQRL--FQEFLAVYDNT--FENS-S LQEQNVQVE---EI 204 WP_000428612 145 DSKEKTDLRLIYLALAHMIKYRGHFLYEDT-FDIKNNDIQKI--FNEFISIYNNT--FEGN-S LSGQNVQVE---AI 212 WP_000428613 145 DSKEKTDLRLIYLALAHMIKYRGHFLYEDT-FDIKNNDIQKI--FSEFISIYDNT--FEGS-S LSGQNAQVE---AI 212 WP_049523028 144 DSKEKVDLRLIYLALAHIIKYRGHFLYEDS-FDIKNNDIQKI--FNEFTILYDNT--FEES-S LSKGNAQVE---EI 211 WP_003107102 113 DSDEKADLRLIYLALAHIIKERGHFLIEGD-LDSQNTDVNAL--FLKLVDTYNLM--FEDD-- IDTQTIDAT---VI 180 WP_054279288 146 DNTEKADLRLIYLALAHIIKFRGHFLIEGA-LSANNTDVQQL--VHALVDAYNIM--FEED-- LDIEAIDVK---AI 213 WP_049531101 145 DSKEKADLRLIYLTLAHMIKYRGHFLYEES-FDIKNNDIQKI--FNEFISIYDNT--FEGS-S LSGQNAQVE---AI 212 WP_049538452 145 DSKEKADLRLIYLALAHMIKYRGHFLYEEA-FDIKNNDIQKI--FNEFINIYDNT--FEGS-S LSGQNEQVE---AI 212 WP_049549711 145 DSKEKADLRLIYLVLAHMIKYRGHFLYEEA-FDIKNNDIQKI--FNEFISIYDNT--FEGS-S LSGQNAQVE---TI 212 WP_007896501 146 DRDQKADLRLIYLALSHIIKFRGHFLIEGK-LNSENTDVQKL--FIALVIVYNLL--FEEE-- IAGETCDAK---AL 213 EFR44625 98 DRDQKADLRLIYLALSHIIKFRGHFLIEGK-LNSENTDVQKL--FIALVIVYNLL--FEEE-- IAGETCDAK---AL 165 WP_002897477 144 DSKEKSDVRLIYLALAHMIKYRGHFLYEET-FDIKNNDIQKI--FNEFINIYDNT--FEGS-S LSGQNAQVE---AI 211 WP_002906454 144 DSKEKTDLRLIYLALAHMIKYRGHFLYEES-FDIKNNDIQKI--FNEFISIYDNT--FEGS-S LSGQNAQVE---AI 211 WP_009729476 145 DSKEKTDLRLIYLALAHMIKYRGHFLYEEA-FDIKNNDIQKI--FNEFISIYNNT--FEGN-S LSGQNVQVE---AI 212 CQR24647 144 DSSEKADLRLVYLALAHIIKYRGHFLIDEP-IDIRNMNSQNL--FKEFLLAFDGI--QVDC-Y LASKHTDIS---GI 211 WP_000066813 145 DSKEKTDLRLIYLALAHMIKYRGHFLYEES-FDIKNNDIQKI--FSEFISIYDNT--FEGK-S LSGQNAQVE---AI 212 WP_009754323 145 DSKEKADLRLIYLALAHITKYRGHFLYEEA-FDIKNNDIQKI--FNEFINIYDNT--FEGS-S LSGQNAQVE---AI 212 WP_044674937 144 DSSQKADIRLIYLALAHIIKYRGHFLFEGD-LKSENKDVQHL--FNDFVEMFDKT--VEGS-Y LSENLPNVA---DV 211 WP_044676715 144 DSSQKADIRLIYLALAHIIKYRGHFLFEGD-LKSENKDVQHL--FNDFVEMFDKT--VEGS-Y LSENLPNVA---DV 211 WP_044680361 144 DSSQKADIRLIYLALAHIIKYRGHFLFEGD-LKSENKDVQHL--FNDFVEMFDKT--VEGS-Y LSENLPNVA---DV 211 WP_044681799 144 DSSQKADIRLIYLALAHIIKYRGHFLFEGD-LKSENKDVQHL--FNDFVEMFDKT--VEGS-Y LSENLPNVA---DV 211 WP_049533112 144 DISQKADLRLVYLALAHMIKFRGHFLIEGQ-LKAENTNVQAL--FKDFVEVYDKT--VEES-H LSEMTVDAL---SI 211 WP_029090905 101 SQHRQFDIREVYLAIHHLIKYRGHFIYEDQtFTIDGNQLQHH--IKAIITMINSTl---NR-- IIPETIDINvfeKI 171 WP_006506696 140 ESTEKADPRLIYLALHHIVKYRGNFLYEGQkFNMDASNIEDK--LSDIFTQFTSFnnIPYEdD --KKNLEIL---EI 210 AIT42264 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_034440723 143 DSNQKADLRLIYLALAHMIKYRGHFLIEGD-LKMDGISISES--FQEFIDSYNEVcaLEDE-N NDELLTQIE---NI 217 AKQ21048 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 WP_004636532 144 DNPEKADLRLVYTALAHIVKYRGHFLIEGE-LNTENTSISET--FEQFLDTYSDI--FKEQ-- LVGDISKVE---EI 210 WP_002364836 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_016631044 95 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENTSVKDQ--FQQFMVIYNQT--FVNGeS PLPESVLIE---EE 168 EMS75795 --------------------------------------------------------------- -------------- WP_002373311 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENTSVKEQ--FQQFMVIYNQT--FVNGeS PLPESVLIE---EE 217 WP_002378009 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_002407324 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_002413717 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_010775580 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_010818269 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_010824395 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENTSVKDQ--FQQFMVIYNQT--FVNGeS PLPESVLIE---EE 217 WP_016622645 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEK--FQQFMIIYNQT--FVNGeG PLPESVLIE---EE 217 WP_033624816 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKDQ--FQQFMVIYNQT--FVNGeS PLPESVLIE---EE 217 WP_033625576 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_033789179 144 DSSEQADLRLIYLALAHIVKYRGHFLIEGK-LSTENISVKEQ--FQQFMIIYNQT--FVNGeS PLPESVLIE---EE 217 WP_002310644 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_002312694 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-G KLDEAVDCS---FV 216 WP_002314015 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_002320716 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_002330729 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_002335161 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_002345439 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_034867970 144 DSTEKEDLRLVYLALAHLLKYRGHFLFEGD-LDTENTSIEES--FRVFLEQYSKQ--SDQP-- -LIVHQPVL---TI 209 WP_047937432 144 DSSEKADIRLVYLAMAHLLKYRGHFLIEGE-LNTENSSVIET--FRQFLSTYNQQ--FSEA-D KLDEAVDCS---FV 216 WP_010720994 144 DSTEKGDLRLVYLALAHLLKYRGHFLFEGD-LDTENTSIEES--FRVFLEQYGKQ--SDQP-- -LIVHQPVL---TI 209 WP_010737004 144 DSTEKEDLRLVYLALAHLLKYRGHFLFEGD-LDTENTSIEES--FRVFLEQYSKQ--SDQP-- -LIVHQPVL---TI 209 WP_034700478 144 DSTEKEDLRLVYLALAHLLKYRGHFLFEGD-LDTENTSIEES--FRVFLEQYGKQ--SDQP-- -LIVHQPVL---TI 209 WP_007209003 144 DGDEKADLRLVYLAIAHIIKFRGNFLIEGE-LNTENNSVIELs--KVFVQLYNQTl-SELE-- FIDESIDFS---EV 214 WP_023519017 144 NSKEQADIRLVYLAIAHCLKYRGHFLFEGE-LDTENTSVIEN--YQQFLQAYQQF--FPEP-- -IGDLDDAV---PI 209 WP_010770040 144 DISEQADLRLVYLALAHIVKYRGHFLIEGE-LNTENSSVSET--FRIFIQVYNQI--FRENe- PLAVPDNIE---EL 212 WP_048604708 144 DAEEKADLRLVYLALAHIIKYRGHFLIEGR-LSTENTSTEET--FKTFLQKYNQT--FN---- PVDETISIG---SI 208 WP_010750235 144 DSTEKADIRLVYLALAHMIKYRGHFLFEGE-LDTENTSVEET--FKEFIDIYNEQ--FEEG-- -IIFYKDIP---LI 209 AII16583 183 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 250 WP_029073316 145 ESKEKEDPRLIYLALHHIVKYRGNFLYEGQkFSMDVSNIEDK--MIDVLRQFNEInlFEYVeD --KKIDEVL---NV 215 WP_031589969 145 ESKEKEDPRLIYLALHHIVKYRGNFLYEGQkFSMDVSNIEDK--MIDVLRQFNEInlFEYVeD --KKIDEVL---NV 215 KDA45870 145 NNDRPADLRLVYLALAHIIKYRGNELLEGE-IDLRITDINKV--FAEFSETLNEN--SDENlG ----KLDVA---DI 209 WP_039099354 133 TEKRQFDIREIYLAMHHIVKYRGHFLNEAPvSSEKSSEINLVahFDRLNTIFADL--FSESgF -TDKLAEVK---AL 206 AKP02966 138 INKNKADIRLVYLALHNILKYRGNFTYEHQkFNISTLNSNLS---KELIELNQQLikYDIS-- -FPDNCDWNhisDI 208 WP_010991369 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTQNTSVDGI--YKQFIQTYNQV--FASGiE KLEDNKDVA---KI 217 WP_033838504 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTQNTSVDGI--YKQFIQTYNQV--FASGiE KLEDNKDVA---KI 217 EHN60060 147 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTQNTSVDGI--YKQFIQTYNQV--FASGiE KLEDNKDVA---KI 220 EFR89594 --------------------------------------------------------------- -------------- WP_038409211 144 NSSDKADLRLVYLALAHIIKYRGNFLIEGM-LDTKNTSVDEV--FKQFIQTYNQI--FASDiE RLEENKEVA---EI 217 EFR95520 --------------------------------------------------------------- -------------- WP_003723650 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDEV--YKQFIETYNQV--FMSNiE KVEENIEVA---NI 217 WP_003727705 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDEV--YKQFIQTYNQV--FMSNiE KVEENTEVA---SI 217 WP_003730785 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDEV--YKQFIQTYNQV--FMSNiE KVEENTEVA---SI 217 WP_003733029 144 DSQKKADLRLVYLALAHIIKYRGHFLIEGA-LDTKNISIDEM--FKQFLQIYNQV--FANDiE KTEKNQEVA---QI 217 WP_003739838 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YKQFIQTYNQV--FISNiE KMEENTTVA---DI 217 WP_014601172 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFIQTYNQV--FMSNiE KVEENIEVA---NI 217 WP_023548323 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFILTYNQV--FMSNiE KVEENIEVA---NI 217 WP_031665337 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFIQTYNQV--FMSNiE KVEENIEVA---NI 217 WP_031669209 144 DSQKKADLRLVYLALAHIIKYRGHFLIEGA-LDTKNISIDEM--FKQFLQIYNQV--FANDiE KTEKNQEVA---QI 217 WP_033920898 144 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFIQTYNQV--FMSNiE KVEENIEVA---NI 217 AKI42028 147 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFIQTYNQV--FMSNiE KVEENIEVA---NI 220 AKI50529 147 NSSEKADLRLVYLALAHIIKYRGNFLIEGA-LDTKNTSVDGV--YEQFIQTYNQV--FMSNiE KVEENIEVA---NI 220 EFR83390 --------------------------------------------------------------- -------------- WP_046323366 144 NSSDKADLRLVYLALAHIIKYRGNFLIEGK-LDTKNTSVDEV--FKQFIKTYNQV--FASDiE RIEENNEVA---KI 217 AKE81011 160 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 227 CUO82355 144 ESTEKADPRLIYLALHHIVKYRGNFLYEGQkFNMDASNIEDK--LSDVFTQFADFnnIPYEdD --KKNLEIL---EI 214 WP_033162887 145 ENKEKADPRLIYLALHHIVKYRGNFLYEGQsFTMDNSDIEER--LNSAIEKFMSIneFDNRiV --SDINSMI---AV 215 AGZ01981 177 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 244 AKA60242 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 AKS40380 144 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 211 4UN5_B 148 DSTDKADLRLIYLALAHMIKFRGHFLIEGD-LNPDNSDVDKL--FIQLVQTYNQL--FEEN-- INASGVDAK---AI 215 WP_010922251 212

277 WP_039695303 213 LTEK-ISKSRRLENLIKY-Y-PT EKKNTLEGNLIALALGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 278 WP_045635197 212 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEDK-A---PLQ--FSKDTYDEDLEN 277 5AXW_A 135 LSTK--------EQISRN-S--K ----------------------------LEEKyVa--ELQ-------------- 157 WP_009880683 ----------------------- ------------------------------------------------------ WP_010922251 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_011054416 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_011284745 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_011285506 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_011527619 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_012560673 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_014407541 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_020905136 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_023080005 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_023610282 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_030125963 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_030126706 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_031488318 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_032460140 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_032461047 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_032462016 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_032462936 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALLLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_032464890 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_033888930 37 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 102 WP_038431314 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_038432938 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-T---KLQ--LSKDTYDDDLDN 277 WP_038434062 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 BAQ51233 123 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 188 KGE60162 ----------------------- ------------------------------------------------------ KGE60856 ----------------------- ------------------------------------------------------ WP_002989955 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_003030002 212 LTEK-VSKSRRLENLIAH-Y-PA EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEG 277 WP_003065552 215 LTEK-ISKSRRLENLIKY-Y-PT EKKNTLEGNLIALALGLQPNEKMNF--KLSED-A---KLQ--FSKDSYEEDLGE 280 WP_001040076 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040078 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040080 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040081 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040083 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040085 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040087 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040088 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040089 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040090 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040091 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040092 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040094 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040095 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040096 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040097 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040098 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040099 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040100 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040104 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040105 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040106 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040107 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040108 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040109 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_001040110 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_015058523 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017643650 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017647151 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017648376 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017649527 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017771611 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_017771984 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 CFQ25032 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 CFV16040 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 KLJ37842 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 KLJ72361 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 KLL20707 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 KLL42645 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_047207273 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_047209694 213 LTDK-ISKSAKKDRILAR-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_050198062 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_050201642 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_050204027 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_050881965 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_050886065 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 AHN30376 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 EAO78426 213 LTDK-ISKSAKKDRILAQ-Y-PN QKSTGIFAEFLKLIVGNQADFKKYF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 CCW42055 213 LTDK-ISKSAKKDRILAQ-Y-PD QKSTGIFAEFLKLIVGNQADFKKHF--NLEDK-T---PLQ--FAKDSYDEDLEN 278 WP_003041502 212 LTEK-VSKSRRLENLIAH-Y-PA EKKNTLEGNLIALFLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEG 277 WP_037593752 213 LTEK-VSKSSRLENLIAH-Y-PT EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEE 278 WP_049516684 213 LTEK-VSKSRRLENLVEC-Y-PT EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEG 278 GAD46167 212 LTEK-VSKSSRLENLIAH-Y-PT EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEE 277 WP_018363470 213 LTEK-ISKSRRLENLINN-Y-PK EKKNTLEGNLIALALGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 278 WP_003043819 212 LSAR-LSKSKRLEKLIAV-F-PN EKKNGLFGNIIALALGLTPNEKSNF--DLTED-A---KLQ--LSKDTYDDDLDE 277 WP_006269658 212 LTEK-VSKSSRLENLIAH-Y-PT EKKNTLEGNLIALSLDLHPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEG 277 WP_048800889 212 LTEK-VSKSRRLENLVKC-Y-PT EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEE 277 WP_012767106 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_014612333 212 LSAR-LSKSKRLENLIAQ-L-PG EKKNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_015017095 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_015057649 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_048327215 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_049519324 212 LSAR-LSKSRRLENLIAQ-L-PG EKRNGLEGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_012515931 212 LSAA-LSKSKRLENLISL-I-PG QKKTGIFGNIIALSLGLTPNEKANF--GLSKD-V---KLQ--LAKDTYADDLDS 277 WP_021320964 212 LSAA-LSKSKRLENLISL-I-PG QKKTGIFGNIIALSLGLTPNEKANF--GLSKD-V---KLQ--LAKDTYADDLDS 277 WP_037581760 212 LSAA-LSKSKRLENLISL-I-PG QKKTGIFGNIIALSLGLTPNEKANF--GLSKD-V---KLQ--LAKDTYADDLDS 277 WP_004232481 212 LTEK-ISKSRRLENLIKQ-Y-PT EKKNTLFGNLVALALGLQPNFKTNF--KLSED-A---KLQ--FSKDTYDEDLEE 277 WP_009854540 213 LTEK-ISKSRRLENLIKY-Y-PT EKKNTLEGNLIALALGLQPNEKMNF--KLSED-A---KLQ--FSKDTYEEDLEE 278 WP_012962174 213 LTEK-FSKSRRLENLIKH-Y-PT EKKNTLFGNLVALALGLQPNFKTSF--KLSED-A---KLQ--FSKDTYEEDLEE 278 WP_039695303 213 LTEK-ISKSRRLENLIKY-Y-PT EKKNTLEGNLIALALGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 278 WP_014334983 212 LTEK-VSKSRRLENLIKQ-Y-PT EKKNTLEGNLIALALGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_003099269 212 LTSK-TSKSRRLENLIAE-I-PN QKRNMLFGNLVSLALGLTPNFKTNF--ELLED-A---KLQ--ISKDSYEEDLDN 277 AHY15608 212 LTSK-TSKSRRLENLIAE-I-PN QKRNMLFGNLVSLALGLTPNFKTNF--ELLED-A---KLQ--ISKDSYEEDLDN 277 AHY17476 212 LTSK-TSKSRRLENLIAE-I-PN QKRNMLFGNLVSLALGLTPNFKTNF--ELLED-A---KLQ--ISKDSYEEDLDN 277 ESR09100 ----------------------- ------------------------------------------------------ AGM98575 212 LTSK-TSKSRRLENLIAE-I-PN QKRNMLFGNLVSLALGLTPNFKTNF--ELLED-A---KLQ--ISKDSYEEDLDN 277 ALF27331 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_018372492 210 FTEN-SSKAKRVETILGL-F-PD ETAAGNLDKFLKLMLGNQADFKKVF--DLEEK----iTLQ--FSKDSYEEDLEL 275 WP_045618028 213 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEDLEN 278 WP_045635197 212 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEDK-A---PLQ--FSKDTYDEDLEN 277 WP_002263549 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002263887 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002264920 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLFRNLVALSLGLQPNFKTNF--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_002269043 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002269448 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002271977 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEDLEE 277 WP_002272766 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEDLEE 277 WP_002273241 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002275430 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002276448 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002277050 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLEGNLIALSLGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_002277364 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002279025 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002279859 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLEGNLIALSLGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEELEV 277 WP_002280230 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002281696 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002282247 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLEGNLIALSLGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_002282906 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002283846 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002287255 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002288990 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002289641 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGCFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002290427 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002295753 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002296423 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002304487 212 LTEK-VSKSRRLENLVEC-Y-PT EKKNTLEGNLIALSLGLQPNEKTNE--QLSED-A---KLQ--FSKDTYEEDLEG 277 WP_002305844 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_002307203 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--LSKDTYEEELEV 277 WP_002310390 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_002352408 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_012997688 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_014677909 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_019312892 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_019313659 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIIGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_019314093 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--LSKDTYEEELEV 277 WP_019315370 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_019803776 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-V---PLQ--FSKDTYEEELEV 277 WP_019805234 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDIYEEELEV 277 WP_024783594 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_024784288 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLEGNLIALSLGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_024784666 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_024784894 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 WP_024786433 212 LTEK-ISKSRRLEKLINN-Y-PK EKKNTLEGNLIALSLGLQPNEKTNE--KLSED-A---KLQ--FSKDTYEEDLEE 277 WP_049473442 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEDLEE 277 WP_049474547 212 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 277 EMC03581 205 LTDK-ISKSAKKDRVLKL-F-PN EKSNGRFAEFLKLIVGNQADFKKHF--ELEEK-A---PLQ--FSKDTYEEELEV 270 WP_000428612 213 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSRDTYDEDLEN 278 WP_000428613 213 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLGEK-A---PLQ--FSKDTYDEDLEN 278 WP_049523028 212 FTDK-ISKSAKRDRVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYEEDLES 277 WP_003107102 181 LTEK-MSKSRRLENLIAK-I-PN QKKNTLEGNLISLSLGLTPNFKANF--ELSED-A---KLQ--ISKESFEEDLDN 246 WP_054279288 214 LTEK-ISKTRRLENLISN-I-PG QKKNGLFGNLIALSLGLTPNEKSHF--NLPED-A---KLQ--LAKDTYDEELNN 279 WP_049531101 213 FTDK-ISKSTKRERVLKL-F-PD QKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEDLEN 278 WP_049538452 213 FSDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEDLEN 278 WP_049549711 213 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLGEK-A---PLQ--FSKDTYDEDLEN 278 WP_007896501 214 LTAK-TSKSKRLESLISE-F-PG QKKNGLFGNLLALALGLRPNFKSNF--GLSED-A---KLQ--ITKDTYEEELDN 279 EFR44625 166 LTAK-TSKSKRLESLISE-F-PG QKKNGLFGNLLALALGLRPNFKSNF--GLSED-A---KLQ--ITKDTYEEELDN 231 WP_002897477 212 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEELEN 277 WP_002906454 212 FTDK-ISKSTKRERVLKL-F-SD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEDLEN 277 WP_009729476 213 FTDK-ISKSAKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSRDTYDEDLEN 278 CQR24647 212 ITAK-ISKSRKVEAVLEQ-F-PD QKKNSFEGNMVSLVEGLMPNEKSNF--ELDED-A---KLQ--FSRDSYDEDLEN 277 WP_000066813 213 FTDK-ISKSTKRERVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---PLQ--FSKDTYDEDLEN 278 WP_009754323 213 FTGK-ISKSVKREHVLKL-F-PD EKSTGLFSEFLKLIVGNQADFKKHF--DLEEK-A---SLQ--FSKDTYDEDLEN 278 WP_044674937 212 LVEK-VSKSRRLENILHY-F-PN EKKNGLFGNFLALALGLQPNFKTNF--ELAED-A---KIQ--FSKETYEEDLEE 277 WP_044676715 212 LVEK-VSKSRRLENILHY-F-PN EKKNGLFGNFLTLALGLQPNFKTNF--ELAED-A---KIQ--FSKETYEEDLEE 277 WP_044680361 212 LVEK-VSKSRRLENILHY-F-PN EKKNGLFGNFLALALGLQPNFKTNF--ELAED-A---KIQ--FSKETYEEDLEE 277 WP_044681799 212 LVEK-VSKSRRLENILHY-F-PN EKKNGLFGNFLALALGLQPNFKTNF--ELAED-A---KIQ--FSKETYEEDLEE 277 WP_049533112 212 LTEK-VSKSRRLENLIAH-Y-PA EKKNTLFGNLIALSLGLQPNEKTNF--QLSED-A---KLQ--FSKDTYEEDLEG 277 WP_029090905 172 LLDRmMNRSSKVKFLIEL---TG KQDKPLLKELFNLIVGLKAKPASIFe---QENlAtivETM-nMSTEQVQLDLLT 243 WP_006506696 211 LKKP-LSKKAKVDEVMTL-IaPE KDYKSAFKELVTGIAGNKMNVTKMI1cEPIKQ-Gds-EIK1kFSDSNYDDQFSE 283 AIT42264 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLFGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_034440723 218 FKQD-ISRSKKLDQAIAL-F-QG -KRQSLFGIFLTLIVGNKANFQKIF--NLEDD----iKLD--LKEEDYDENLEE 283 AKQ21048 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLFGNLIALSLGLTPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 WP_004636532 211 LSSK-QSRSRKHEQIMAL-F-PN ENKLGNFGRFMMLIVGNTSNFKPVF--DLDDE-Y---KLK--LSDETYEEDLDT 276 WP_002364836 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_016631044 169 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 234 EMS75795 1 ----------------------- ----------------------------MDEE-A---KIQ--LSKESYEEELES 20 WP_002373311 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_002378009 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_002407324 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_002413717 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_010775580 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KIKitYASESYEEDLEG 285 WP_010818269 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_010824395 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_016622645 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_033624816 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_033625576 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_033789179 218 LTEK-ASRTKKSEKVLQQ-F-PQ EKANGLFGQFLKLMVGNKADFKKVF--GLEEE-A---KI--tYASESYEEDLEG 283 WP_002310644 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EE-A---KLQ--FSKETYEEDLEE 281 WP_002312694 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_002314015 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_002320716 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_002330729 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EE-A---KLQ--FSKETYEEDLEE 281 WP_002335161 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_002345439 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_034867970 210 LTDK-LSKIKKVEEILKY-Y-PT EKINSFFAQCLKLIVGNQANFKRIF--DLEAE-V---KLQ--FSKETYEEDLES 275 WP_047937432 217 FTEK-MSKIKKAEILLKY-F-PH EKSNGYLSQFIKLMVGNQGNFKNVF--GL-EEeA---KLQ--FSKETYEEDLEE 282 WP_010720994 210 LTDK-LSKIKKVEEILKY-Y-PT EKINSFFAQCLKLIVGNQANFKRIF--DLEAE-V---KLQ--FSKETYEEDLES 275 WP_010737004 210 LTDK-LSKIKKVEEILKY-Y-PT EKINSFFAQCLKLIVGNQANFKRIF--DLEAE-V---KLQ--FSKETYEEDLES 275 WP_034700478 210 LTDK-LSKIKKVEEILKY-Y-PT EKINSFFAQCLKLIVGNQANFKRIF--DLEAE-V---KLQ--FSKETYEEDLES 275 WP_007209003 215 LTQQ-LSKSERADNVLKL-F-PD EKGIGIFAQFIKLIVGNQGNFKKVF--QLEED----qKLQ--LSTDDYEENIEN 280 WP_023519017 210 LTER-LSKAKRVEKVLAY-Y-PS EKSTGNFAQFLKLMVGNQANFKKTF--DLEEE-M---KLN--FTRDCYEEDLNE 275 WP_010770040 213 FSEK-VSRARKVEAILSV-Y-SE EKSTGTLAQFLKLMVGNQGRFKKTF--DLEED-G---IIQ--IPKEEYEEELET 278 WP_048604708 209 FADK-VSRAKKAEGVLAL-F-PD EKRNGTFDQFLKMIVGNQGNFKKTF--ELEED-A---KLQ--FSKEEYDESLEA 274 WP_010750235 210 LTDK-LSKSKKVEKILQY-Y-PK EKTIGCLAQFLKLIVGNQGNFKQAF--HLDEE-V---KIQ--ISKETYEEDLEK 275 AII16583 251 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLFGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 316 WP_029073316 216 LKEP-LSKKHKADKAFAL-FdTT KDNKAAYKELCAALAGNKENVIKMLkeAELHD-EdekDISfkFSDATFDDAFVE 289 WP_031589969 216 LKEP-LSKKHKAEKAFAL-FdTT KDNKAAYKELCAALAGNKENVIKMLkeAELHD-EdekDISfkFSDATFDDAFVE 289 KDA45870 210 FKDNtFSKTKKSEELLKL---SG -KKNQLAHQLFKMMVGNMGSFKKVL--GTDEE----hKLS--FGKDTYEDDLND 275 WP_039099354 207 LLDNhQSASNRQRQALLLiYtPS KQNKAIATELLKAILGLKAKFNVLT--GIEAEdVktwTLT--FNAENFDEEMVK 285 AKP02966 209 LIGR-GNATQKSSNILNN-F--T KETKKLLKEVINLILGNVAHLNTIFktSLIKDeE---KLS--FSGKDIESKLDD 278 WP_010991369 218 LVEK-VTRKEKLERILKL-Y-PG EKSAGMFAQFISLIVGSKGNFQKPF--DLIEK-S---DIE--CAKDSYEEDLES 283 WP_033838504 218 LVEK-VTRKEKLERILKL-Y-PG EKSAGMFAQFISLIVGSKGNFQKPF--DLIEK-S---DIE--CAKDSYEEDLES 283 EHN60060 221 LVEK-VTRKEKLERILKL-Y-PG EKSAGMFAQFISLIVGSKGNFQKPF--DLIEK-S---DIE--CAKDSYEEDLES 286 EFR89594 1 ---------------LKL-Y-PG EKSTGMFAQFISLIVGSKGNFQKPF--DLIEK-S---DIE--CAKDSYEEDLES 52 WP_038409211 218 LSEK-LTRREKLDKILKL-Y-TG EKSIGMFARFINLIIGSKGDFKKVF--DLDEK-A---EIE--CAKDIYEEDLEA 283 EFR95520 ----------------------- ------------------------------------------------------ WP_003723650 218 LAGK-FTRREKFERILQL-Y-PG EKSIGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLET 283 WP_003727705 218 LAGK-FTRREKFERILRL-Y-PG EKSIGMFAQFISLIVGNKGNFQKVF--NLVEK-T---DIE--CAKDSYEEDLEA 283 WP_003730785 218 LAGK-FTRREKFERILRL-Y-PG EKSIGMFAQFISLIVGNKGNFQKVF--NLVEK-T---DIE--CAKDSYEEDLEA 283 WP_003733029 218 LAEK-FTRKDKLDKILSL-Y-PG EKTIGVFAQFVNIIVGSTGKEKKHF--NLHEK-K---DIN--CAEDTYDIDLES 283 WP_003739838 218 LAGK-FTRKEKLERILQL-Y-PG EKSIGMFAQFISLIVGSKGNFQKVF--DLVEK-T---DIE--CAKDSYEEDLEA 283 WP_014601172 218 LAGK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLEA 283 WP_023548323 218 LAGK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLET 283 WP_031665337 218 LAGK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLET 283 WP_031669209 218 LAEK-FTRKDKLDKILSL-Y-PG EKTTGVFAQFVNIIVGSTGKEKKHF--NLHEK-K---DIN--CAEDTYDIDLES 283 WP_033920898 218 LARK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLET 283 AKI42028 221 LAGK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLEA 286 AKI50529 221 LARK-FTRREKFERILQL-Y-PG EKSTGMFAQFISLIVGSKGNFQKVF--DLIEK-T---DIE--CAKDSYEEDLET 286 EFR83390 ----------------------- ------------------------------------------------------ WP_046323366 218 FSEK-LTKREKLDKILNL-Y-PN EKSTDLFAQFISLIIGSKGNEKKFF--NLTEK-T---DIE--CAKDSYEEDLEV 283 AKE81011 228 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 293 CUO82355 215 LKKP-LSKKAKVDEVMAL-IsPE KEEKSAYKELVTGIAGNKMNVTKMIlcESIKQ-Gds-EIKlkFSDSNYDDQFSE 287 WP_033162887 216 LSKI-YQRSKKADDLLKI-MnPT KEEKAAYKEFTKALVGLKENISKMIlaQEVKK-Gdt-DIVleFSNANYDSTIDE 288 AGZ01981 245 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 310 AKA60242 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 AKS40380 212 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 277 4UN5_B 216 LSAR-LSKSRRLENLIAQ-L-PG EKKNGLEGNLIALSLGLIPNEKSNF--DLAED-A---KLQ--LSKDTYDDDLDN 281 WP_010922251 278

356 WP_039695303 279 LLGKIGDDYADLFTSAKNLYDAILLSGILTVDDNSTKAPLSASMIKRYVEHHEDLEKLKEFIKAN-KSELYHDIFKDKNK 357 WP_045635197 278 LLGQIGDDFIDLFVSAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQNDLAALKQFIKNN-LPEKYDEVFSDQSK 356 5AXW_A 158 ---------------------------------------------------------LERLKKDG-------EVR----- 168 WP_009880683 1 ---------------------------------------LSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 40 WP_010922251 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_011054416 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_011284745 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_011285506 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_011527619 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_012560673 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_014407541 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_020905136 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_023080005 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_023610282 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_030125963 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKASLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_030126706 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_031488318 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_032460140 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_032461047 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_032462016 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_032462936 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_032464890 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_033888930 103 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 181 WP_038431314 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_038432938 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_038434062 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 BAQ51233 189 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 267 KGE60162 -------------------------------------------------------------------------------- KGE60856 -------------------------------------------------------------------------------- WP_002989955 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_003030002 278 LLGEIGDEYADLFASAKNLYDAILLSGILTVDDNSTKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APDQYNAIFKDKNK 356 WP_003065552 281 LLGKIGDDYADLFTSAKNLYDAILLSGILIVDDNSTKAPLSASMIKRYVEHQEDLEKLKEFIKAN-KSELYHDIFKDKNK 359 WP_001040076 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIVADSSK 357 WP_001040078 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040080 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040081 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040083 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040085 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040087 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040088 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040089 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040090 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040091 279 LLRQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040092 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSAYMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040094 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQHYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040095 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040096 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040097 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040098 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040099 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040100 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040104 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040105 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_001040106 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_001040107 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_001040108 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_001040109 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_001040110 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_015058523 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_017643650 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_017647151 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_017648376 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_017649527 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_017771611 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVTALSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_017771984 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 CFQ25032 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKASLSDSMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 CFV16040 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 KLJ37842 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 KLJ72361 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 KLL20707 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 KLL42645 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_047207273 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_047209694 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_050198062 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_050201642 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_050204027 279 LLGQIGDEFADLFSVAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFTDSSK 357 WP_050881965 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_050886065 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 AHN30376 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 EAO78426 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 CCW42055 279 LLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLKQLKQFVKAS-LPEKYQEIFADSSK 357 WP_003041502 278 LLGEVGDEYADLFASAKNLYDAILLSGILTVDDNSTKAPLSASMVKRYEEHQKDLKKFEDFIKVN-ALDQYNAIFKDKNK 356 WP_037593752 279 LLGEIGDEYADLFASAKNLYDAILLSGILAVDDNITKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APDQYNAIFKDKNK 357 WP_049516684 279 LLGEIGDEYADLFASAKNLYDAILLSGILAVDDNITKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APAQYDDIFKDETK 357 GAD46167 278 LLGEIGDEYADLFASAKNLYDAILLSGILAVDDNITKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APDQYNAIFKDKNK 356 WP_018363470 279 LLGKIGDDYADLFTSSKNLYDAILLSGILTVDDNSTKAPLSASMIKRYVEHHEDLEKLKEFIKAN-KSELYHDIFKDKTQ 357 WP_003043819 278 LLGQIGDQYADLFSAAKNLSDAILLSDILRSNSEVTKAPLSASMVKRYDEHHQDLALLKTLVRQQ-FPEKYAEIFKDDTK 356 WP_006269658 278 FLGEVGDEYADLFASAKNLYDAILLSGILTVDDNSTKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APDQYNAIFKDKNK 356 WP_048800889 278 LLGKIGDDYADLFTSAKNLYDTILLSGILAVDDNSTKALLSASMIKRYEEHQKDLKKLKDFIKVN-APAQYDDIFKDETK 356 WP_012767106 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_014612333 278 LLAQIGNQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_015017095 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_015057649 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_048327215 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_049519324 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_012515931 278 LLAQIGDQYADLFLAAKNLSDAILLSDILTESDEITRAPLSASMVKRYREHHKDLVTLKTLIKDQ-LPEKYQEIFLDKTK 356 WP_021320964 278 LLAQIGDQYADLFLAAKNLSDAILLSDILTESDEITRAPLSASMVKRYREHHKDLVTLKTLIKDQ-LPEKYQEIFLDKTK 356 WP_037581760 278 LLAQIGDQYADLFLAAKNLSDAILLSDILTESDEITRAPLSASMVKRYREHHKDLVTLKTLIKDQ-LPEKYQEIFLDKTK 356 WP_004232481 278 LLGKIGDDYADLETAAKNLYDAILLSGILTVDDNSTKAPLSASMIKRYEEHHEDLEKLKTFIKVN-NEDKYHEIFKDKSK 356 WP_009854540 279 LLGKIGDDYADLFTSAKNLYDAILLSGILTVDDNSTKAPLSASMIKRYVEHHEDLEKLKEFIKAN-KSELYHDIFKDKNK 357 WP_012962174 279 LIGKIGDEYADLFTSAKNLYDAILLSGILTVADNITKAPLSASMIKRYNEHQVDLKKLKEFIKNN-ASDKYDEIFNDKDK 357 WP_039695303 279 LLGKIGDDYADLFTSAKNLYDAILLSGILTVDDNSTKAPLSASMIKRYVEHHEDLEKLKEFIKAN-KSELYHDIFKDKNK 357 WP_014334983 278 LLGKVGDDYADLFISAKNLYDAILLSGILTVDDNSTKAPLSASMIKRYVEHHEDLEKLKEFIKIN-KLKLYHDIFKDKIK 356 WP_003099269 278 LLAQIGDQYADLFIAAKKLSDAILLSDIITVKGASTKAPLSASMVQRYEEHQQDLALLKNLVKKQ-IPEKYKEIFDNKEK 356 AHY15608 278 LLAQIGDQYADLFIAAKKLSDAILLSDIITVKGASTKAPLSASMVQRYEEHQQDLALLKNLVKKQ-IPEKYKEIFDNKEK 356 AHY17476 278 LLAQIGDQYADLFIAAKKLSDAILLSDIITVKGASTKAPLSASMVQRYEEHQQDLALLKNLVKKQ-IPEKYKEIFDNKEK 356 ESR09100 -------------------------------------------------------------------------------- AGM98575 278 LLAQIGDQYADLFIAAKKLSDAILLSDIITVKGASTKAPLSASMVQRYEEHQQDLALLKNLVKKQ-IPEKYKEIFDNKEK 356 ALF27331 278 LLAQIEDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_018372492 276 LLSKIDEEYAALFDLAKKVYDAVLLSNILTVKEKNTKAPLSASMIKRYEEHKDDLKAFKRFFRER-LPEKYETMEKDLTK 354 WP_045618028 279 LLVQIGDDFADLELVAKKLYDAILLSGILTVTDPSTKAPLSASMIDRYENHQKDLAALKQFIKTN-LPEKYDEVESDQSK 357 WP_045635197 278 LLGQIGDDFIDLEVSAKKLYDAILLSGILTVTDPSTKAPLSASMIERYENHQNDLAALKQFIKNN-LPEKYDEVESDQSK 356 WP_002263549 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002263887 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002264920 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIKRYAEHHEDLEKLKEFIKAN-KPELYHDIFKDETK 356 WP_002269043 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002269448 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002271977 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVESDVSK 356 WP_002272766 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVESDVSK 356 WP_002273241 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002275430 278 LLTQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002276448 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002277050 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIKRYAEHHEDLEKLKEFIKAN-KPELYHDIFKDETK 356 WP_002277364 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002279025 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTADDSSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002279859 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002280230 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002281696 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002282247 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIKRYAEHHEDLEKLKEFIKAN-KPELYHDIFKDETK 356 WP_002282906 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002283846 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002287255 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002288990 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002289641 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLTQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002290427 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002295753 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002296423 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002304487 278 LLGEIGDEYADLFASAKNLYDAILLSGILAVDDNITKAPLSASMVKRYKEHKEELAAFKRFIKEK-LPKKYEEIFKDDTK 356 WP_002305844 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002307203 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002310390 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_002352408 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_012997688 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_014677909 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019312892 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019313659 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTQAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019314093 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019315370 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLTQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019803776 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_019805234 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_024783594 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVGTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_024784288 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIKRYAEHHEDLEKLKEFIKAN-KPELYHDIFKDETK 356 WP_024784666 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLVQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_024784894 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVTDVSTKAPLSASMIQRYNEHQMDLTQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_024786433 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIKRYAEHHEDLEKLKEFIKAN-KPELYHDIFKDETK 356 WP_049473442 278 LLGKIGDDYADLFTLAKNLYDAILLSGILTADDSSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 356 WP_049474547 278 LLAQIGDNYAELFLSAKKLYDSILLSGILTVIDVSTKAPLSASMIQRYNEHQMDLTQLKQFIRQK-LSDKYNEVFSDVSK 356 EMC03581 271 LLAQIGDNYAELFLSAKKLYDSILLSGILTVIDVSTKAPLSASMIQRYNEHQMDLAQLKQFIRQK-LSDKYNEVFSDVSK 349 WP_000428612 279 LLGQIGDDFADLEVAAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQKDLATLKQFIKTN-LPEKYDEVFSDQSK 357 WP_000428613 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQKDLAVLKQFIKNN-LPEKYDEVFSDQSK 357 WP_049523028 278 LLGQIGDVYADLFVVAKKLYDAILLAGILSVKDPGIKAPLSASMIERYDNHQNDLSALKQFVRRN-LPEKYAEVFSDDSK 356 WP_003107102 247 LLAQIGDQYADLFIAAKNLSDAILLSDILTVKGVNTKAPLSASMVQRFNEHQDDLKLLKKLVKVQ-LPEKYKEIFDIKDK 325 WP_054279288 280 LLTQIGDEYADLELSAKNLSDAILLSDILIVNGDGIQAPLSASLIKRYEEHRQDLALLKQMFKEQ-LPDLYRDVFIDENK 358 WP_049531101 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQKDLAALKQFIKNN-LPEKYDEVFSDQSK 357 WP_049538452 279 LLGQIGDGFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYQNHQNDLASLKQFIKNN-LPEKYDEVFSDQSK 357 WP_049549711 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQKDLITLKQFIKNN-LPEKYDEVFSDQSK 357 WP_007896501 280 LLAEIGDHYADLFLAAKNLSDAILLSDILTLSDENTRAPLSASMIKRYEEHQEDLALLKKLVKEQ-MPEKYWEIFSNAKK 358 EFR44625 232 LLAEIGDHYADLFLAAKNLSDAILLSDILTLSDENTRAPLSASMIKRYEEHQEDLALLKKLVKEQ-MPEKYWEIFSNAKK 310 WP_002897477 278 LLGQIGDDFADLFLIAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQKDLAALKQFIKNN-LPEKYVEVFSDQSK 356 WP_002906454 278 LLGQIGDGFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQEDLAALKQFIKNN-LSEKYAEVFSDQSK 356 WP_009729476 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVINPSTKAPLSASMIERYENHQKDLASLKQFIKNN-LPEKYDEVFSDQSE 357 CQR24647 278 LLGIIGDEYADVFVAAKKVYDSILLSGILTINNHSTKAPLSASMIDRYDEHNSDKKLLRDFIRTNiGKEVFKEVFYDISK 357 WP_000066813 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVKDLSTKAPLSASMIERYENHQKDLAALKQFIQNN-LQEKYDEVFSDQSK 357 WP_009754323 279 LLGQIGDDFADLFLVAKKLYDAILLSGILTVIDPSTKAPLSASMIERYENHQEDLAALKQFIKNN-LPEKYAEVFSDQSK 357 WP_044674937 278 LLGKIGDDYADLFIATKSLYDGILLAGILSTIDSTIKAPLSSSMVNRYEEHKKDLALLKNFIHQN-LSDSYKEVFNDKLK 356 WP_044676715 278 LLGKIGDDYADLFIATKSLYDGILLAGILSTIDSTIKAPLSSSMVNRYEEHQKDLALLKNFIHQN-LSDSYKEVFNDKLK 356 WP_044680361 278 LLGKIGDDYADLFIATKSLYDGILLAGILSTIDSTIKAPLSSSMVNRYEEHQKDLALLKNFIHQN-LSDSYKEVFNDKLK 356 WP_044681799 278 LLGKIGDDYADLFIATKSLYDGILLAGILSTIDSTIKAPLSSSMVNRYEEHKKDLALLKNFIHQN-LSDSYKEVFNDKLK 356 WP_049533112 278 LLGEIGDEYADLFASAKNLYDAILLSGILTVDDNSTKAPLSASMVKRYEEHQKDLKKLKDFIKVN-APDQYNAIFKDKNK 356 WP_029090905 244 LADVLADEEYDLLLTAQKIYSAIILDESMDGYEYFA-----EAKKESYRKHQEELVLVKKMLKSNaIINDERAKF---EY 315 WP_006506696 284 VEKDLGE-YVEFVDALHNVYSWVELQIIMGATHTD-NASISEAMVSRYNKHHDDLKLLKDCIKNN-VPNKYFDMERNDSE 360 AIT42264 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_034440723 284 LLSNIDEGYRDVFLQAKNVYNAIELSKILKIDGKETKAPLSAQMVELYNQHREDLKKYKDYIKAY-LPEKYGETFKDATK 362 AKQ21048 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 356 WP_004636532 277 LLGMTDDVFLDVFMAAKNVYDAVEMSAIISTDIGNSKAVLSNQMINFYDEHKVDLAQLKQFFKTH-LPDKYYECFSDPSK 355 WP_002364836 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_016631044 235 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 313 EMS75795 21 LLEKSGEEFRDVFLQAKKVYDAILLSDILSIKKQNSKAKLSLGMIERYDSHKKDLEELKQFVKAN-LPEKTAIFFKDSSK 99 WP_002373311 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_002378009 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 362 WP_002407324 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 362 WP_002413717 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_010775580 286 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 364 WP_010818269 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_010824395 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 362 WP_016622645 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSYAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_033624816 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 362 WP_033625576 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKKFKRFIREN-CPDEYDNLFKNEQK 362 WP_033789179 284 ILAKVGDEYSDVFLAAKNVYDAVELSTILADSDKKSHAKLSSSMIVRFTEHQEDLKNFKRFIREN-CPDEYDNLFKNEQK 362 WP_002310644 282 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 360 WP_002312694 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_002314015 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_002320716 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_002330729 282 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 360 WP_002335161 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_002345439 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_034867970 276 LLEKIGDEYLDIFLQAKKVHDAILLSEIISSIVKHTKAKLSSGMVERYERHKADLAKFKQFVKEN-VPQKATVFFKDITK 354 WP_047937432 283 LLEKIGDDYIDLFVQAKNVYDAVLLSEILSDSTKNTRAKLSAGMIRRYDAHKEDLVLLKRFVKEN-LPKKYRAFFGDNSV 361 WP_010720994 276 LLEKIGDEYLDIFLQAKKVHDAILLSEIISSIVKHTQAKLSSGMVERYERHKADLAKFKQFVKEN-VPQKATVFFKDITK 354 WP_010737004 276 LLEKIGDEYLDIFLQAKKVHDAILLSEIISSIVKHTKAKLSSGMVERYERHKADLAKFKQFVKEN-VPQKATVFFKDITK 354 WP_034700478 276 LLEKIGDEYLDIFLQAKKVHDAILLSEIISSIVKHTQAKLSSGMVERYERHKADLAKFKQFVKEN-VPQKATVFFKDITK 354 WP_007209003 281 LLAIIGDEYGDIFVAAQNLYQAILLAGILTSTEK-TRAKLSASMIQRYEEHAKDLKLLKRFVKEH-IPDKYAEIFNDATK 358 WP_023519017 276 LLEKTSDDYAELFLKAKGVYDAILLSQILSKSDDETKAKLSANMKLRFEEHQRDLKQLKELVRRD-LPKKYDDFFKNRSK 354 WP_010770040 279 LLAIIGDEYAEIFSATKSVYDAVALSGILSVIDGDTKAKLSASMVERYEAHQKDLVQFKQFIRKE-LPEMYAPIERDNSV 357 WP_048604708 275 LLGEIGDEYADVFEAAKNVYNAVELSGILTVIDNSTKAKLSASMIKRYEDHKIDLKLEKEFIRKN-LPEKYHEIENDKNT 353 WP_010750235 276 LLRKSNEEMIDVFLQVKKVYDAILLSDILSTKMKDTKAKLSAGMIERYQNHKKDLEELKQFVRAH-LHEKVIVFFKDSSK 354 AII16583 317 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLILLKALVRQQ-LPEKYKEIFFDQSK 395 WP_029073316 290 KQPLLGD-CVEFIDLLHDIYSWVELQNILGSAHTS-EPSISAAMIQRYEDHKNDLKLLKDVIRKY-LPKKYFEVERDEKS 366 WP_031589969 290 KQPLLGD-CVEFIDLLHDIYSWVELQNILGSAHTS-EPSISAAMIQRYEDHKNDLKLLKDVIRKY-LPKKYFEVERDEKS 366 KDA45870 276 LLAEAGDQYLDIEVAAKKVYDAAILASILDVKDTQTKIVESQAMIERYEEHQKDLIELKRVEKKY-LPEKCHDFFSE-PK 353 WP_039099354 286 LESSLDDNAHQIIESLQELYSGVLLAGIVPENQSLS-----QAMITKYDDHQKHLKMLKAVREAL-APEDRQRLKQAYDQ 359 AKP02966 279 LDSILDDDQFTVLDTANRIYSTITLNEIL-----NGESYFSMAKVNQYENHAIDLCKLRDMWHTT----KNEKAV-GLSR 348 WP_010991369 284 LLALIGDEYAELEVAAKNAYSAVVLSSIITVAETETNAKLSASMIERFDTHEEDLGELKAFIKLH-LPKHYEEIFSNTEK 362 WP_033838504 284 LLALIGDEYAELEVAAKNAYSAVVLSSIITVAETETNAKLSASMIERFDTHEEDLGELKAFIKLH-LPKHYEEIFSNTEK 362 EHN60060 287 LLALIGDEYAELEVAAKNAYSAVVLSSIITVAETETNAKLSASMIERFDTHEEDLGELKAFIKLH-LPKHYEEIFSNTEK 365 EFR89594 53 LLALIGDEYAELEVAAKNAYSAVVLSSIITVAETETNAKLSASMIERFDTHEEDLGELKAFIKLH-LPKHYEEIFSNTEK 131 WP_038409211 284 LLAKIGDEYAEIEVAAKSTYNAVVLSNIITVIDTETKAKLSASMIERFDKHAKDLKRLKAFFKMQ-LPEKENEVENDIEK 362 EFR95520 -------------------------------------------------------------------------------- WP_003723650 284 LLAIIGDEYAELEVAAKNTYNAVVLSSIITVIDTETNAKLSASMIERFDAHEKDLVELKAFIKLN-LPKQYEEIFSNAAI 362 WP_003727705 284 LLAIIGDEYAELEVAAKNTYNAVVLSSIITVTATETNAKLSASMIERFDAHEKELGELKAFIKLH-LPKQYQEIENNAEI 362 WP_003730785 284 LLAIIGDEYAELEVAAKNTYNAVVLSSIITVTATETNAKLSASMIERFDAHEKELGELKAFIKLH-LPKQYQEIENNAEI 362 WP_003733029 284 LLAIIGDEFAEVEVAAKNAYNAVVLSNIITVIDSTTRAKLSASLIERFENHKEDLKKMKRFVRTY-LPEKYDEIFDDTEK 362 WP_003739838 284 LLAIIGDEYAELEVAAKNTYNAVVLSSIITVIDTETNAKLSASMIERFDAHEKDLSELKAFIKLH-LPKQYEEIFSNVAI 362 WP_014601172 284 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVTATETNAKLSASMIERFDAHEKDLGELKAFIKLH-LPKQYQEIFNNAAI 362 WP_023548323 284 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVTDTETNAKLSASMIERFDAHEKDLVELKAFIKLN-LPKQYEEIFSNAAI 362 WP_031665337 284 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVNDTETNAKLSASMIERFDAHEKDLVELKAFIKLN-LPKQYEEIFSNAAI 362 WP_031669209 284 LLAIIGDEFAEVFVAAKNAYNAVVLSNIITVTDSTTRAKLSASLIERFENHKEDLKKMKRFVRTY-LPEKYDEIFDDTEK 362 WP_033920898 284 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVTDTETNAKLSASMIERFDAHEKDLVELKAFIKLN-LPKQYEEIFSNAAI 362 AKI42028 287 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVTATETNAKLSASMIERFDAHEKDLGELKAFIKLH-LPKQYQEIFNNAAI 365 AKI50529 287 LLAIIGDEYAELFVAAKNTYNAVVLSSIITVTDTETNAKLSASMIERFDAHEKDLVELKAFIKLN-LPKQYEEIFSNAAI 365 EFR83390 -------------------------------------------------------------------------------- WP_046323366 284 LLARVGDEYAEIFVAAKNAYNAVVLSSIITVSNTETKAKLSASMIERFDKHDKDLKRMKAFFKVR-LPENFNEVENDVEK 362 AKE81011 294 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 372 CUO82355 288 VENDLGE-YVEFIDSLHNIYSWVELQTIMGATHTD-NASISEAMVSRYNKHHEDLQLLKKCIKDN-VPKKYFDMERNDSE 364 WP_033162887 289 LQSELGE-YIEFIEMLHNIYSWVELQAILGATHTD-NPSISAAMVERYEEHKKDLRVLKKVIREE-LPDKYNEVFRKDNR 365 AGZ01981 311 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 389 AKA60242 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 356 AKS40380 278 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 356 4UN5_B 282 LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ-LPEKYKEIFFDQSK 360 WP_010922251 357

419 WP_039695303 358 --NGYAG YIEN G VKQDEFYKYLKNILSK-IkiDGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 422 WP_045635197 357 --DGYAG YIDG K TIQETFYKYIKNLLSK-F--EGTDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 5AXW_A 169 ------G SINR - ---------------K------TSDYVk----------------------------EA 183 WP_009880683 41 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 103 WP_010922251 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_011054416 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPYQIHLGEL 419 WP_011284745 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_011285506 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_011527619 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_012560673 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_014407541 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_020905136 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_023080005 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPYQIHLGEL 419 WP_023610282 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPYQIHLGEL 419 WP_030125963 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_030126706 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPYQIHLGEL 419 WP_031488318 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_032460140 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_032461047 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_032462016 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_032462936 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_032464890 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNRKDLLRKQRTFDNGSIPHQIHLGEL 419 WP_033888930 182 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 244 WP_038431314 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_038432938 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPYQIHLGEL 419 WP_038434062 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 BAQ51233 268 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 330 KGE60162 ------- ---- - ---------------------------------------------------------- KGE60856 ------- ---- - ---------------------------------------------------------- WP_002989955 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_003030002 357 --KGYAG YIEN G VKQDEFYKYLKGILLQ-I--NGSGDFL--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_003065552 360 --NGYAG YIEN G VKQDEFYKYLKNILSK-Ia--GSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 422 WP_001040076 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040078 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040080 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040081 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040083 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040085 358 --DGYAG YIEG K INQEAFYKYLSKLLIK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040087 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040088 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040089 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040090 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040091 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040092 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040094 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040095 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040096 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040097 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040098 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040099 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040100 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040104 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040105 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040106 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040107 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040108 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040109 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_001040110 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_015058523 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017643650 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017647151 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017648376 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017649527 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017771611 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_017771984 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 CFQ25032 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 CFV16040 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 KLJ37842 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 KLJ72361 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 KLL20707 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 KLL42645 358 --DGYAG YIEG K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_047207273 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_047209694 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_050198062 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_050201642 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_050204027 358 --DGYAG YIES K TNQGAFYKYLSKLLTK-Q--EGSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_050881965 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_050886065 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 AHN30376 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSEYFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 EAO78426 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EDSENFL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 CCW42055 358 --DGYAG YIEG K TNQEAFYKYLSKLLTK-Q--EGSEYLL--EKIKNEDFLRKQRTFDNGSIPHQVHLTEL 420 WP_003041502 357 --KGYAG YIES G VKQDEFYKYLKGILLQ-I--NGSGDFL--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_037593752 358 --KGYAG YIES G VEQDEFYKYLKGILLK-I--NGSGDFL--DKIDCEDFLRKQRTFDNGSIPHQIHLQEM 420 WP_049516684 358 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--DGSDYFL--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 420 GAD46167 357 --KGYAG YIES G VEQDEFYKYLKGILLK-I--NGSGDFL--DKIDCEDFLRKQRTFDNGSIPHQIHLQEM 419 WP_018363470 358 --NGYAG YIEN G VKQDEFYKYLKGILTK-I--NGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 420 WP_003043819 357 --NGYAG YVGI G ATQEEFYKFIKPILEK-M--DGAEELLa--KLNRDDLLRKQRTFDNGSIPHQIHLKEL 429 WP_006269658 357 --KGYAS YIES G VKQDEFYKYLKGILLK-I--NGSGDFL--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_048800889 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--DGSGYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_012767106 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_014612333 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_015017095 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_015057649 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_048327215 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_049519324 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLa--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 WP_012515931 357 --NGYAG YIEG Q VSQEEFYKYLKPILAR-L--DGSEPLL1--KIDREDFLRKQRTEDNGSIPHQIHLEEL 419 WP_021320964 357 --NGYAG YIEG Q VSQEEFYKYLKPILAR-L--DGSEPLL1--KIDREDFLRKQRTEDNGSIPHQIHLEEL 419 WP_037581760 357 --NGYAG YIEG Q VSQEEFYKYLKPILAR-L--DGSEPLL1--KIDREDFLRKQRTEDNGSIPHQIHLEEL 419 WP_004232481 357 --NGYAG YIEN G VKQDIFYKHLKSIISE-K--NGGQYFL--DKIEREDFLRKQRTFDNGSIPYQIHLQEM 419 WP_009854540 358 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--DGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 420 WP_012962174 358 --NGYAG YIEN G VKQDEFYKYLKTTLSK-I--DGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 420 WP_039695303 358 --NGYAG YIEN G VKQDEFYKYLKNILSK-IkiDGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 422 WP_014334983 357 --NGYAG YIDN G VKQDEFYKYLKTILTK-I--DDSDYFL--DKIERDDFLRKQRTFDNGSIPHQIHLQEM 419 WP_003099269 357 --NGYAG YIDG K TSQEEFYKYIKPILLK-L--DGTEKLIs--KLEREDFLRKQRTFDNGSIPHQIHLNEL 419 AHY15608 357 --NGYAG YIDG K TSQEEFYKYIKPILLK-L--DGTEKLIs--KLEREDFLRKQRTFDNGSIPHQIHLNEL 419 AHY17476 357 --NGYAG YIDG K TSQEEFYKYIKPILLK-L--DGTEKLIs--KLEREDFLRKQRTFDNGSIPHQIHLNEL 419 ESR09100 ------- ---- - ---------------------------------------------------------- AGM98575 357 --NGYAG YIDG K TSQEEFYKYIKPILLK-L--DGTEKLIs--KLEREDFLRKQRTFDNGSIPHQIHLNEL 419 ALF27331 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_018372492 355 --PSYAA YVSG A VTEDDFYKFSKGLLID-V--EGAEYFL--EKIEREDFLRKQRTFDNGAIPNQVHVKEL 432 WP_045618028 358 --DGYAG YIDG K TTQEAFYKYIKNLLSK-L--EGADYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 420 WP_045635197 357 --DGYAG YIDG K TTQETFYKYIKNLLSK-F--EGIDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_002263549 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_002263887 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_002264920 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--AGSDYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_002269043 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_002269448 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002271977 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002272766 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002273241 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002275430 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002276448 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002277050 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--AGSDYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002277364 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002279025 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002279859 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002280230 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002281696 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002282247 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--TGSDYFL--DQIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002282906 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002283846 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002287255 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002288990 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002289641 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002290427 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002295753 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002296423 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002304487 357 --NGYAG YVGA D ATEEEFYKYVKGILNK-V--EGADVWL--DKIDREDFLRKQRTEDNGSIPHQIHLQEM 429 WP_002305844 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002307203 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002310390 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002352408 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_012997688 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_014677909 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019312892 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019313659 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019314093 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019315370 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGNGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019803776 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_019805234 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_024783594 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_024784288 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--TGSDYFL--DQIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_024784666 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_024784894 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_024786433 357 --NGYAG YIEN G VKQDEFYKYLKNTLSK-I--AGSDYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_049473442 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_049474547 357 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 EMC03581 350 --DGYAG YIDG K TNQEAFYKYLKGLLNK-I--EGSGYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 412 WP_000428612 358 --DGYAG YIDG K TTQESFYKYIKNLLSK-F--EGADYFL--EKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_000428613 358 --DGYAG YIDG K TTQEAFYKYIKNLLSK-F--EGTDYFL--EKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_049523028 357 --DGYAG YIDG K TTQEGFYKYIKNLISK-I--EGAEYFL--EKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_003107102 326 --NGYAG YING K TSQEDFYKYIKPILSK-L--KGAESLIs--KLEREDFLRKQRTFDNGSIPHQIHLNEL 388 WP_054279288 359 --DGYAG YISG K TSQEAFYKYIKPILET-L--DGAEDFLt--KINREDFLRKQRTEDNGSIPHQIHLGEL 421 WP_049531101 358 --EGYAG YIDS K TTQEAFYKYIKNLLSK-I--DGADYLL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_049538452 358 --DGYAG YVDG K TTQEAFYKYIKNLLSK-F--EGADYFL--EKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_049549711 358 --DGYAG YIDG K TTQEAFYKYIKNLLSK-F--EGTDYFL--EKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_007896501 359 --NGYAG YIEG K VSQEDFYRYIKPILSR-L--KGGDEFLa--KIDRDDFLRKQRTEDNGSIPHQIHLKEL 421 EFR44625 311 --NGYAG YIEG K VSQEDFYRYIKPILSR-L--KGGDEFLa--KIDRDDFLRKQRTEDNGSIPHQIHLKEL 373 WP_002897477 357 --DGYAG YIDG K TTQEAFYKYIKNLLSK-F--EGADYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_002906454 357 --DGYAG FIDG K TTQEAFYKYIKNLLSK-L--EGADYFL--NKIEREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_009729476 358 --DGYAG YIDG K TTQETFYKYIKNLLSK-F--EGADYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 CQR24647 358 --NGYAG YIDG K TNQEDFYKYLKNLLQK-V--DGGDYFI--EKIEREDFLRKQRTFDNGSIPHQVHLDEM 420 WP_000066813 358 --DGYAG YIDG K TTQEAFYKYIKNLLSK-F--EGADYFL--DKIEREDFLKKQRTEDNGSIPHQIHLQEM 420 WP_009754323 358 --DGYAG YIDG K TTQEAFYKYIKNLLSK-F--EGADYFL--DKIEREDFLRKQRTEDNGSIPHQIHLQEM 420 WP_044674937 357 --DGYAG YIEG K TTQENFYRFIKKAIEK-I--EGSDYFI--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_044676715 357 --DGYAG YIEG K TTQENFYRFIKKAIEK-I--EGSNYFI--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_044680361 357 --DGYAG YIEG K TTQENFYRFIKKAIEK-I--EGSNYFI--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_044681799 357 --DGYAG YIEG K TTQENFYRFIKKAIEK-I--EGSDYFI--DKIDREDFLRKQRTFDNGSIPHQIHLQEM 419 WP_049533112 357 --KGYAG YIEN G VKQDEFYKYLKGILLQ-I--NGSGDFL--DKIDREDFLRKQRTEDNGSIPHQIHLQEM 419 WP_029090905 316 fyIDYIG YEES K SKEERLEKHIELLLAKeNvlTTVEHALleKNITFASLLPLQRSSRNAVIPYQVHEKEL 403 WP_006506696 361 ksKGYYN YINR K APVDEFYKYVKKCIEK-VdtPEAKQILn--DIELENFLLKQNSRINGSVPYQMQLDEM 429 AIT42264 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_034440723 363 --NGYAG YIDG K TSQEDFYKFVKAQLKG---eENGEYFL--EAIENENFLRKQRSFYNGVIPYQIHLQEL 425 AKQ21048 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 419 WP_004636532 356 --NGYAG YIDG K TNQEDFYKYIEKVMKT-IksDKKDYFL--DKIDREVFLRKQRSFYNSVIPHQIHLQEM 420 WP_002364836 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_016631044 314 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTEDNGVIPHQIHLAEL 378 EMS75795 100 --NGYAG YIDG K TTQEDFYKFLKKELNG-I--AGSERFM--EKVDQENFLLKQRTTANGVIPHQVHLTEL 162 WP_002373311 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_002378009 363 --DGYAG YITH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_002407324 363 --DGYAG YITH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_002413717 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_010775580 365 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTEDNGVIPHQIHLAEL 429 WP_010818269 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_010824395 363 --DGYAG YITH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_016622645 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_033624816 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_033625576 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_033789179 363 --DGYAG YIAH A VSQLKFYQYVKKIIQD-I--AGAEYFL--EKIAQENFLRKQRTFDNGVIPHQIHLAEL 427 WP_002310644 361 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 423 WP_002312694 362 --NGYAG YIEG H ATQEAFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLSEL 424 WP_002314015 362 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 424 WP_002320716 362 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 424 WP_002330729 361 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 423 WP_002335161 362 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 424 WP_002345439 362 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 424 WP_034867970 355 --NGYAG YIKG K TIQEEFYKFVKKELSG-V--VGSEPFL--EKIDQETFLLKQRTYINGVIPHQVHLIEL 417 WP_047937432 362 --NGYAG YIEG H ATQEDFYKFVKKELTG-I--RGSEVFL--TKIEQENFLRKQRTFDNGVIPHQIHLTEL 424 WP_010720994 355 --NGYAG YIKG K TIQEEFYKFVKKELSG-V--VGSEPFL--EKIDQETFLLKQRTYINGVIPHQVHLIEL 417 WP_010737004 355 --NGYAG YIKG K TIQEEFYKFVKKELSG-V--VGSEPFL--EKIDQETFLLKQRTYINGVIPHQVHLIEL 417 WP_034700478 355 --NGYAG YIKG K TIQEEFYKFVKKELSG-V--VGSEPFL--EKIDQETFLLKQRTYINGVIPHQVHLIEL 417 WP_007209003 359 --NGYAG YIDG K TKEEEFYKYLKTTLVQ---kSGYQYFI--EKIEQENFLRKQRIYDNGVIPHQVHAEEL 421 WP_023519017 355 --NGYAG YVKG K ATQEDFYKFLRTELAG-L--EESQSIM--EKIDLEIYLLKQRTFANGVIPHQIHLVEM 417 WP_010770040 358 --SGYAG YVEN S VTQAEFYKYIKKAIEK-V--PGAEYFL--EKIEQETFLDKQRTENNGVIPHQIHLEEL 422 WP_048604708 354 --DGYAG YIDN S ISQEKFYKYITNLIEK-I--DGAEYFL--KKIENEDFLRKQRTFDNGIIPHQIHLEEL 418 WP_010750235 355 --DGYAG YIDG K TTQADFYKFLKKELTG-V--PGSEPML--AKIDQENFLLKQRTPINGVIPHQVHLTEF 417 AII16583 396 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTEDNGSIPHQIHLGEL 458 WP_029073316 367 kkNNYCN YINH K TPVDEFYKYIKKLIEK-IddPDVKTILn--KIELESFMLKQNSRINGAVPYQMQLDEL 435 WP_031589969 367 kkNNYCN YINH K TPVDEFYKYIKKLIEK-IddPDVKTILn--KIELESFMLKQNSRINGAVPYQMQLDEL 435 KDA45870 354 -iSGYAG YIDG K VSEEDFYKYIKKILKG-I--PETEEILq--KIDANNYLRKQRTEDNGAIPHQVHLKEL 417 WP_039099354 360 ------- YVDG K -SKEDFYGDITKALKNnPdhPIVSEIKk--LIELDQFMPKQRTKDNGAIPHQLHQQEL 425 AKP02966 349 --QAYDD YINK K ---KELYISLKKELKVaLp-INLAKEAe-EKISKGTYLVKPRNSENGVVPYQLNKIEM 415 WP_010991369 363 --HGYAG YIDG - TKQADFYKYMKMTLEN-I--EGADYFI--AKIEKENFLRKQRTFDNGAIPHQLHLEEL 425 WP_033838504 363 --HGYAG YIDG - TKQADFYKYMKMTLEN-I--EGADYFI--AKIEKENFLRKQRTFDNGAIPHQLHLEEL 425 EHN60060 366 --HGYAG YIDG - TKQADFYKYMKMTLEN-I--EGADYFI--AKIEKENFLRKQRTFDNGAIPHQLHLEEL 428 EFR89594 132 --HGYAG YIDG - TKQADFYKYMKTTLEN-I--EGADYFI--AKIEKENFLRKQRTFDNGAIPHQLHLEEL 194 WP_038409211 363 --DGYAG YIDG - TIQEKFYKYMKKMLAN-I--DGADYFI--DQIEEENFLRKQRTFDNGTIPHQLHLEEL 425 EFR95520 1 ------- ---- - ---------MKKMLAN-I--DGADYFI--DQIEEENFLRKQRTFDNGTIPHQLHLEEL 44 WP_003723650 363 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGSDYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 425 WP_003727705 363 --DGYAG YIDG - TKQVDFYKYLKTTLEN-V--EGADYFI--TKIEEENFLRKQRTFDNGVIPHQLHLEEL 425 WP_003730785 363 --DGYAG YIDG - TKQVDFYKYLKTTLEN-V--EGADYFI--TKIEEENFLRKQRTFDNGVIPHQLHLEEL 425 WP_003733029 363 --HGYAG YISG - TKQADFYKYMKATLEK-I--EGADYFI--AKIEEENFLRKQRTEDNGVIPHQLHLEEL 425 WP_003739838 363 --DGYAG YIDG - TKQVDFYKYLKILLEN-I--EGADYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 425 WP_014601172 363 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGADYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 425 WP_023548323 363 --DGYAG YIDG - TKQVDFYKYLKTTLEN-V--EGADYFI--TKIEEENFLRKQRTFDNGVIPHQLHLEEL 425 WP_031665337 363 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGSDYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 425 WP_031669209 363 --HGYAG YISG - TKQADFYKYMKATLEK-I--EGADYFI--AKIEEENFLRKQRTFDNGVIPHQLHLEEL 425 WP_033920898 363 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGADYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 425 AKI42028 366 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGADYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 428 AKI50529 366 --DGYAG YIDG - TKQVDFYKYLKTILEN-I--EGADYFI--AKIEEENFLRKQRTFDNGAIPHQLHLEEL 428 EFR83390 ------- ---- - ---------------------------------------------------------- WP_046323366 363 --DGYAG YIEG - TKQEAFYKYMKKMLEH-V--EGADYFI--NQIEEENFLRKQRTFDNGAIPHQLHLEEL 425 AKE81011 373 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTFDNGSIPHQIHLGEL 435 CUO82355 365 kvKGYYN YINR K APVDEFYKFVKKCIEK-VdtPEAKQILh--DIELENFLLKQNSRINGSVPYQMQLDEM 433 WP_033162887 366 klHNYLG YIKY D TPVEEFYKYIKGLLAK-VdtDEAREILe--RIDLEKFMLKQNSRTNGSIPYQMQKDEM 434 AGZ01981 390 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTFDNGSIPHQIHLGEL 452 AKA60242 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 AKS40380 357 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTFDNGSIPHQIHLGEL 419 4UN5_B 361 --NGYAG YIDG G ASQEEFYKFIKPILEK-M--DGTEELLv--KLNREDLLRKQRTFDNGSIPHQIHLGEL 423 WP_010922251 420

486 WP_039695303 423 HAILRRQGDYYPFLKE--KQD RIEKILTFRIPYYVGPL VRKD--SRFAWAEY---RSDEKITPWNFDKVIDKEK 489 WP_045635197 420 NAILRRQGEYYPFLKD--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDEAIRPWNFEEIVDKAS 486 5AXW_A 184 KQLLKVQKAYHQLDQSfi--D TYIDLLETRRTYYEGPG ---Eg-SPFGWKDI---------------------- 229 WP_009880683 104 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 170 WP_010922251 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_011054416 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_011284745 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_011285506 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_011527619 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_012560673 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_014407541 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_020905136 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_023080005 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_023610282 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_030125963 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_030126706 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_031488318 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_032460140 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_032461047 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_032462016 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_032462936 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_032464890 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_033888930 245 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 311 WP_038431314 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_038432938 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_038434062 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 BAQ51233 331 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 397 KGE60162 --------------------- ----------------- ------------------------------------ KGE60856 --------------------- ----------------- ------------------------------------ WP_002989955 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_003030002 420 HAILRRQEEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_003065552 423 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARKD--SRFSWAEY---HSDEKITPWNFDKVIDKEK 489 WP_001040076 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040078 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040080 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040081 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040083 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040085 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040087 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040088 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040089 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040090 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040091 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040092 421 KAIIRRQSEYYPFLKE--NQD KIEKILTFRIPYYVGPL ARGN--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040094 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040095 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040096 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040097 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040098 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040099 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040100 421 RAIIRRQSEYYPLLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040104 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040105 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_001040106 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_001040107 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_001040108 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_001040109 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_001040110 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_015058523 421 KAIIRRQSEYYPFLKE--NQD KIEKILTFRIPYYVGPL ARGN--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_017643650 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_017647151 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_017648376 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_017649527 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_017771611 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_017771984 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 CFQ25032 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 CFV16040 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 KLJ37842 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 KLJ72361 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 KLL20707 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 KLL42645 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_047207273 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_047209694 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_050198062 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_050201642 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_050204027 421 KAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_050881965 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 WP_050886065 421 KDIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 AHN30376 421 KAIIRRQSEYYPFLKE--NQD KIEKILTFRIPYYVGPL ARGN--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 EAO78426 421 KAIIRRQSEYYPFLKE--NQD RIEKILTFRIPYYIGPL AREK--SDFAWMTR---KTDDSIRPWNFEDLVDKEK 487 CCW42055 421 RAIIRRQSEYYPFLKE--NLD RIEKILTFRIPYYVGPL AREK--SDFAWMTR---KTDDSIRPWNFEELVDKEA 487 WP_003041502 420 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_037593752 421 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 487 WP_049516684 421 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 487 GAD46167 420 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_018363470 421 HAILRRQGDYYPFLKE--NQE EIEKILTFRIPYYVGPL ARKD--SRFAWAEY---RSDEKITPWNFDKVIDKEK 487 WP_003043819 430 HAILRRQEEFYPFLKE--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWLTR---KSEEAITPWNFEEVVDKGA 496 WP_006269658 420 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_048800889 420 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL VRKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_012767106 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_014612333 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_015017095 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_015057649 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_048327215 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_049519324 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_012515931 420 HAILRRQEVEYPFLKD--NRK KIESLLTFRIPYYVGPL ARG-h-SRFAWVKR---KFDGAIRPWNFEEIVDEEA 486 WP_021320964 420 HAILRRQEVEYPFLKD--NRK KIESLLTFRIPYYVGPL ARG-h-SRFAWVKR---KFDGAIRPWNFEEIVDEEA 486 WP_037581760 420 HAILRRQEVEYPFLKD--NRK KIESLLTFRIPYYVGPL ARG-h-SRFAWVKR---KFDGAIRPWNFEEIVDEEA 486 WP_004232481 420 RTILRRQGEYYPFLKE--NQA KIEKILTFRIPYYVGPL ARKN--SRFAWAKY---HSDEPITPWNFDEVVDKEK 486 WP_009854540 421 HAILRRQGDYYPFLKE--KQD RIEKILTFRIPYYVGPL VRKD--SRFAWAEY---RSDEKITPWNFDKVIDKEK 487 WP_012962174 421 HAILRRQGEHYAFLKE--NQA KIEKILTFRIPYYVGPL ARKN--SRFAWAEY---HSDEKITPWNFDEIIDKEK 487 WP_039695303 423 HAILRRQGDYYPFLKE--KQD RIEKILTFRIPYYVGPL VRKD--SRFAWAEY---RSDEKITPWNFDKVIDKEK 489 WP_014334983 420 HSILRRQGDYYPFLKE--NQA KIEKILTFRIPYYVGPL ARKD--SRFAWANY---HSDEPITPWNFDEVVDKEK 486 WP_003099269 420 KAIIRRQEKEYPFLKE--NQK KIEKLFTFKIPYYVGPL ANG-q-SSFAWLKR---QSNESITPWNFEEVVDQEA 486 AHY15608 420 KAIIRRQEKEYPFLKE--NQK KIEKLFTFKIPYYVGPL ANG-q-SSFAWLKR---QSNESITPWNFEEVVDQEA 486 AHY17476 420 KAIIRRQEKEYPFLKE--NQK KIEKLFTFKIPYYVGPL ANG-q-SSFAWLKR---QSNESITPWNFEEVVDQEA 486 ESR09100 --------------------- ----------------- ------------------------------------ AGM98575 420 KAIIRRQEKEYPFLKE--NQK KIEKLFTFKIPYYVGPL ANG-q-SSFAWLKR---QSNESITPWNFEEVVDQEA 486 ALF27331 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYIGPL ARGK--SDFSWLSR---KSADKITPWNFDEIVDKES 486 WP_018372492 433 QAIILNQSKYYPFLAE--NKE KIEKILTFRIPYYVGPL ARGN--SSFAWLQR---KSDEAIRPWNFEQVVDMET 499 WP_045618028 421 NAIIRRQGEHYPFLQE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAR 487 WP_045635197 420 NAILRRQGEYYPFLKD--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDEAIRPWNFEEIVDKAS 486 WP_002263549 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002263887 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002264920 420 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002269043 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002269448 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002271977 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002272766 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002273241 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002275430 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002276448 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002277050 420 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARKN--SRFAWAEY---HSDEAVMPWNFDQVIDKES 486 WP_002277364 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002279025 420 RAIIRRQSEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002279859 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002280230 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002281696 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002282247 420 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARKN--SRFAWAEY---HSDEAVTPWNFDQVIDKES 486 WP_002282906 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002283846 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002287255 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002288990 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002289641 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ASGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002290427 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002295753 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002296423 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002304487 430 HAILRRQGEHYPFLKE--NQD KIEKILTFRIPYYVGPL VRKG--SRFAWAEY---KADEKITPWNFDDILDKEK 496 WP_002305844 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002307203 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002310390 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_002352408 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_012997688 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_014677909 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019312892 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019313659 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019314093 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019315370 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019803776 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_019805234 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_024783594 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_024784288 420 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARKN--SRFAWAEY---HSDEAVTPWNFDQVIDKES 486 WP_024784666 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_024784894 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ASGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_024786433 420 HAILRRQGDYYPFLKE--NQD RIEKILTFRIPYYVGPL ARKN--SRFAWAEY---HSDEAVTPWNFDQVIDKES 486 WP_049473442 420 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 WP_049474547 420 RAIIRRQAEFYPFLAD--NQD RIEKLLTFRIPYYVGPL ASGK--SDFAWLSR---KSADKITPWNFDEIVDKES 486 EMC03581 413 RAIIRRQAEFYPFLAD--NQD RIEKILTFRIPYYVGPL ARGK--SDFAWLSR---KSADKITPWNFDEIVDKES 479 WP_000428612 421 NAILRRQGEHYPFLKE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 WP_000428613 421 NAILRRQGEHYPFLKD--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDEAIRPWNFEEIVDKAS 487 WP_049523028 420 NAILRHQGEYYPFLKE--NKD KIEQILTFRIPYYVGPL ARGN--SDFAWLSR---NSDEAIRPWNFEEMVDKSS 486 WP_003107102 389 KSIIRRQEKYYPFLKD--KQV RIEKIFTFRIPYFVGPL ANG-n-SSFAWVKR---RSNESITPWNFEEVVEQEA 455 WP_054279288 422 QAILERQQAYYPFLKD--NQE KIEKILTFRIPYYIGPL ARG-n-SRFAWLTR---TSDQKITPWNFDEMVDQEA 488 WP_049531101 421 NAILRRQGEHYPFLKE--NRE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 WP_049538452 421 NAILRRQGEHYPFLKE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 WP_049549711 421 NAILRRQGEHYPFLKE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 WP_007896501 422 HAILRRQEKYYPFLAE--QKE KIEQLLCFRIPYYVGPL AKGGn-SSFAWLKR---RSDEPITPWNFKDVVDEEA 489 EFR44625 374 HAILRRQEKYYPFLAE--QKE KIEQLLCFRIPYYVGPL AKGGn-SSFAWLKR---RSDEPITPWNFKDVVDEEA 441 WP_002897477 420 NAILRRQGEHYPFLKE--NRE KIEKILTFRIPYYVGPL ARDN--RDFSWLTR---NSDEPIRPWNFEEVVDKAR 486 WP_002906454 420 NAILRRQGEHYLFLKE--NRE KIEKILAFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEVVDKAS 486 WP_009729476 421 NAILRRQGEHYPFLKE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 CQR24647 421 KAILRRQGEFYPFLKE--NAE KIQQILTFKIPYYVGPL ARGN--SRFAWASY---NSNEKMTPWNFDNVIDKTS 487 WP_000066813 421 NAIIRRQGEHYPFLQE--NKE KIEKILTFRIPYYVGPL ARGN--GDFAWLTR---NSDQAIRPWNFEEIVDQAS 487 WP_009754323 421 NAILRRQGEHYPLLKE--NKE KIEKILTFRIPYYVGPL ARGN--RDFAWLTR---NSDQAIRPWNFEEIVDKAS 487 WP_044674937 420 HAIIRRQAEFYPFLVE--NQD KIEKILTFRIPYYVGPL ARGK--SEFAWLNR---KSDEKIRPWNFDEMVDKET 486 WP_044676715 420 HAIIRRQAEFYPFLVE--NQD KIEKILTFRIPYYVGPL ARGK--SEFAWLNR---KSDEKIRPWNFDEMVDKET 486 WP_044680361 420 HAIIRRQAEFYPFLVE--NQD KIEKILTFRIPYYVGPL ARGK--SEFAWLNR---KSDEKIRPWNFDEMVDKET 486 WP_044681799 420 HAIIRRQAEFYPFLVE--NQD KIEKILTFRIPYYVGPL ARGK--SEFAWLNR---KSDEKIRPWNFDEMVDKET 486 WP_049533112 420 HAILRRQEEHYPFLKE--NQD KIEKILTFRIPYYVGPL ARKG--SRFAWAEY---KADEKITPWNFDDILDKEK 486 WP_029090905 404 VAILENQATYYPFLLE--QKD NIHKLLTFRIPYYVGPL ADQKd-SEFAWMVR---KQAGKITPFNFEEMVDIDA 471 WP_006506696 430 IKIIDNQAEYYPILKE--KRE QLLSILTFRIPYYFGPL ETSEh----AWIKRlegKENQRILPWNYQDIVDVDA 498 AIT42264 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_034440723 426 TAVLDQQEKHYSFLKE--NRD KIISLLTFRIPYYVGPL AKGE--SRFAWLER--sNSEEKIKPWNFDKIVDIDK 493 AKQ21048 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 WP_004636532 421 QAILDRQSQYYPFLAE--NRD KIESLVTFRIPYYVGPL TVSDg-SEFAWMER---QSDEPIRPWNFDEIVNKER 488 WP_002364836 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_016631044 379 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 446 EMS75795 163 KAIIERQKPYYPSLEE--ARD KMIRLLTFRIPYYVGPL AQGEetSSFAWLER---KTPEKVTPWNATEVIDYSA 231 WP_002373311 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_002378009 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-NTFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_002407324 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_002413717 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_010775580 430 QAIIHRQAAYYPFLKE--NQK KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 497 WP_010818269 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_010824395 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_016622645 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_033624816 428 QAIIHRQAAYYPFLKE--NQK KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_033625576 428 QAIIHRQAAYYPFLKE--NQE KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QNEKPIRPWNLQETVDLDQ 495 WP_033789179 428 QAIIHRQAAYYPFLKE--NQK KIEQLVTFRIPYYVGPL SKGDa-STFAWLKR---QSEEPIRPWNLQETVDLDQ 495 WP_002310644 424 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 492 WP_002312694 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_002314015 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_002320716 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_002330729 424 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 492 WP_002335161 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_002345439 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_034867970 418 KAIIDQQKQHYPFLEE--AGP KIIALFKFRIPYYVGPL AKEQeaSSFAWIER---KTAEKINPWNFSEVVDIEK 486 WP_047937432 425 RAIIANQKKHYPFLKE--EQE KLESLLTFKIPYYVGPL AKKQenSPFAWLIR---KSEEKIKPWNLPEIVDMEG 493 WP_010720994 418 KAIIDQQKQHYPFLEE--AGP KIIALFKFRIPYYVGPL AKEQeaSSFAWIER---KTAEKINPWNFSEVVDIEK 486 WP_010737004 418 KAIIDQQKQHYPFLEE--AGP KIIALFKFRIPYYVGPL AKEQeaSSFAWIER---KTAEKINPWNFSEVVDIEK 486 WP_034700478 418 KAIIDQQKQHYPFLEE--AGP KIIALFKFRIPYYVGPL AKEQeaSSFAWIER---KTAEKINPWNFSEVVDIEK 486 WP_007209003 422 RAILRKQEKYYSFLKE--NHE KIEQIFKVRIPYYVGPL AKHNegSRFAWNIR---KSDEPIRPWNMNDVVDENA 490 WP_023519017 418 REIMDRQKRFYPFLKG--AQG KIEKLLTFRIPYYVGPL AQEGg-SPFAWIKR---KSPSQITPWNFAEVVDKEN 485 WP_010770040 423 EAIIQKQATYYPFLAD--NKE EMKQLVTFRIPYYVGPL ADGN--SPFAWLER---ISSEPIRPGNLAEVVDIKK 489 WP_048604708 419 KAILHHQAMYYPFLQE--KFS NFVDLLTFRIPYYVGPL ANGN--SRFSWLSR---KSDEPIRPWNLAEVVDLSK 485 WP_010750235 418 KAIIDQQKQYYPFLEK--SKE KMIQLLTFRIPYYVGPL AQDKetSSFAWLER---KTTEKIKPWNAKDVIDYGA 486 AII16583 459 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 525 WP_029073316 436 NKILENQSVYYSDLKD--NED KIRSILTFRIPYYFGPL ITKDr--QFDWIIKkegKENERILPWNANEIVDVDK 506 WP_031589969 436 NKILENQSVYYSDLKD--NED KIRSILTFRIPYYFGPL ITKDr--QFDWIIKkegKENERILPWNANEIVDVDK 506 KDA45870 418 VAIVENQGKYYPFLRE--NKD KFEKILNFRIPYYVGPL ARGN--SKFAWLTR--a-GEGKITPYNFDEMIDKET 484 WP_039099354 426 DRIIENQQQYYPWLAE-1NPN KLDELVAFRVPYYVGPL QQQSsdAKFAWMIR---KAEGQIIPWNFDDKVDRQA 509 AKP02966 416 EKIIDNQSQYYPFLKE--NKE KLLSILSFRIPYYVGPL -QSSekNPFAWMER---KSNGHARPWNFDEIVDREK 483 WP_010991369 426 EAILHQQAKYYPFLKE--NYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_033838504 426 EAILHQQAKYYPFLKE--NYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 EHN60060 429 EAILHQQAKYYPFLKE--NYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 495 EFR89594 195 EAILHQQAKYYPFLKE--NYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 261 WP_038409211 426 EAILHQQAKYYPFLRK--DYE KIRSLVTFRIPYFIGPL ANGQ--SDFAWLTR---KADGEIRPWNIEEKVDFGK 492 EFR95520 45 EAILHQQAKYYPFLRK--DYE KIRSLVTFRIPYFIGPL ANGQ--SDFAWLTR---KADGEIRPWNIEEKVDFGK 111 WP_003723650 426 EAIIHQQAKYYPFLKE--DYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_003727705 426 EAILHQQAKYYPFLRE--GYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KDDGEIRPWNIEEKVDFGK 492 WP_003730785 426 EAILHQQAKYYPFLRE--GYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KDDGEIRPWNIEEKVDFGK 492 WP_003733029 426 EAILHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_003739838 426 EAILHQQAKYYPFLKE--AYD KIKSLVTFRIPYFVGPL ANGQ--SDFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_014601172 426 EAIIHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_023548323 426 EAILHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_031665337 426 EAIIHQQAKYYTELKE--DYD KIKSLVTFRIPYFVGPL ANGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_031669209 426 EAILHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 WP_033920898 426 EAIIHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 492 AKI42028 429 EAIIHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 495 AKI50529 429 EAIIHQQAKYYPFLRE--DYE KIKSLVTFRIPYFVGPL AKGQ--SEFAWLTR---KADGEIRPWNIEEKVDFGK 495 EFR83390 --------------------- ----------------- ------------------------------------ WP_046323366 426 EAILHQQAKYYPFLKV--DYE KIKSLVTFRIPYFVGPL ANGQ--SEFSWLTR---KADGEIRPWNIEEKVDFGK 492 AKE81011 436 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 502 CUO82355 434 IKIIDNQAKYYPVLKE--KRE QLLSILTFRIPYYFGPL ETSEh----AWIKRlegKENQRILPWNYQDTVDVDA 502 WP_033162887 435 IQIIDNQSVYYPQLKE--NRD KLISILEFRIPYYFGPL AHSE----FAWIKKfedKQKERILPWNYDQIVDIDA 503 AGZ01981 453 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 519 AKA60242 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 AKS40380 420 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 486 4UN5_B 424 HAILRRQEDFYPFLKD--NRE KIEKILTFRIPYYVGPL ARG-n-SRFAWMTR---KSEETITPWNFEEVVDKGA 490 WP_010922251 487

561 WP_039695303 490 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETYAVYNELTKIKYVN--EQGKES-FFDSNMKQEIFDHVEK--ENR-KVTK 563 WP_045635197 487 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--ENR-KVTE 561 5AXW_A 230 --KEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITR--DENEKLeYYE---KFQIIENVFK--QKK-KPTL 299 WP_009880683 171 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 245 WP_010922251 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_011054416 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_011284745 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_011285506 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_011527619 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_012560673 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_014407541 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_020905136 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_023080005 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_023610282 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_030125963 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_030126706 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_031488318 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGEQKKAIVDLLFK--TNR-KVTV 561 WP_032460140 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_032461047 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_032462016 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_032462936 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_032464890 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_033888930 312 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 386 WP_038431314 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_038432938 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_038434062 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 BAQ51233 398 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 472 KGE60162 -------------------------------------------------------------------------------- KGE60856 -------------------------------------------------------------------------------- WP_002989955 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_003030002 487 SAEKFITRMTLNDLYLPEEKVLPKHSLLYETFTVYNELTKVKYVN--EQGEAK-FFDANMKQEIFDHVEK--ENR-KVTK 560 WP_003065552 490 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETYAVYNELTKIKYVN--EQGKDS-FFDSNMKQEIFDHVEK--ENR-KVTK 563 WP_001040076 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040078 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040080 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040081 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040083 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040085 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040087 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040088 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040089 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040090 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040091 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040092 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNVKQEIFDGVFK--EHR-KVSK 561 WP_001040094 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040095 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040096 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040097 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040098 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040099 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040100 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_001040104 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040105 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040106 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040107 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040108 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040109 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_001040110 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_015058523 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNVKQEIFDGVFK--EHR-KVSK 561 WP_017643650 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_017647151 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_017648376 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_017649527 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_017771611 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_017771984 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 CFQ25032 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 CFV16040 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 KLJ37842 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 KLJ72361 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 KLL20707 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 KLL42645 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_047207273 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_047209694 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLA--EGFKDFqFLNRKQKETIENSLEK--EKR-KVTE 562 WP_050198062 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_050201642 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_050204027 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_050881965 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVFK--EHR-KVSK 561 WP_050886065 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVEK--EHR-KVSK 561 AHN30376 488 SAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNVKQEIFDGVEK--EHR-KVSK 561 EAO78426 488 SAEAFIHRMTNNDFYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVEK--EHR-KVSK 561 CCW42055 488 SAEAFIHCMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRYKN--EQGETY-FFDSNIKQEIFDGVEK--EYR-KVSK 561 WP_003041502 487 SAEKFITRMTLNDLYLPEEKVLPKHSLLYETFTVYNELTKVKYVN--EQGEAK-FFDANMKQEIFDHVEK--ENR-KVTK 560 WP_037593752 488 SAEKFITRMTLNDLYLPEEKVLPKHSPLYETFTVYNELTKVKYVN--EQGEAK-FFDTNMKQEIFDHVEK--ENR-KVTK 561 WP_049516684 488 SAEKFITRMTLNDLYLPEEKVLPKHSPLYETFTVYNELTKVKYVN--EQGEAK-FFDTNMKQEIFDHVEK--ENR-KVTK 561 GAD46167 487 SAEKFITRMTLNDLYLPEEKVLPKHSPLYETFTVYNELTKVKYVN--EQGEAK-FFDTNMKQEIFDHVEK--ENR-KVTK 560 WP_018363470 488 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETFAVYNELTKVKYVN--EQGKDS-FFDSNMKQEIFDHVEK--ENR-KVTK 561 WP_003043819 497 SAQSFIERMTNEDEQLPNKKVLPKHSLLYEYFTVYNELTKVKYVT--ERMRKPeFLSGEQKKAIVDLLFK--TNR-KVTV 571 WP_006269658 487 SAEKFITRMTLNDLYLPEEKVLPKHSPLYEAFTVYNELTKVKYVN--EQGEAK-FFDTNMKQEIFDHVEK--ENR-KVTK 560 WP_048800889 487 SAEKFITRMTLNDLYLPEEKVLPKHSLLYEIFTVYNELTKVKYVN--EQGEAK-FFDANMKQEIFDHVEK--ENP-KVTK 560 WP_012767106 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGKQKEAIVDLLFK--TNR-KVTV 561 WP_014612333 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGKQKEAIVDLLFK--TNR-KVTV 561 WP_015017095 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGKQKEAIVDLLFK--TNR-KVTV 561 WP_015057649 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGKQKEAIVDLLFK--TNR-KVTV 561 WP_048327215 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPeFLSGKQKEAIVDLLFK--TNR-KVTV 561 WP_049519324 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_012515931 487 SAQIFIEKMTKNDLYLPNEKVLPKHSLLYETFTVYNELTKVKYAT--EGMTRPqFLSADQKQAIVDLLFK--TNR-KVTV 561 WP_021320964 487 SAQIFIEKMTKNDLYLPNEKVLPKHSLLYETFTVYNELTKVKYAT--EGMTRPqFLSADQKQAIVDLLFK--TNR-KVTV 561 WP_037581760 487 SAQIFIEKMTKNDLYLPNEKVLPKHSLLYETFTVYNELTKVKYAT--EGMTRPqFLSADQKQAIVDLLFK--TNR-KVTV 561 WP_004232481 487 SAEKFITRMTLNDLYLPEEKVLPKHSYVYETFAVYNELTKIKYVN--EQGKSF-FFDANMKQEIFDHVEK--ENR-KVTK 560 WP_009854540 488 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETYAVYNELTKIKYVN--EQGKES-FFDSNMKQEIFDHVEK--ENR-KVTK 561 WP_012962174 488 SAEKFITRMTLNDLYLPEEKVLPKHSLVYETYTVYNELTKVKYVN--EQGKSN-FFDANMKQEIFEHVEK--ENR-KVTK 561 WP_039695303 490 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETYAVYNELTKIKYVN--EQGKES-FFDSNMKQEIFDHVEK--ENR-KVTK 563 WP_014334983 487 SAEKFITRMTLNDLYLPEEKVLPKHSHVYETFTVYNELTKIKYVN--EQGESF-FFDANMKQEIFDHVEK--ENR-KVTK 560 WP_003099269 487 SARAFIERMTNEDTYLPEEKVLPKHSPLYEMFMVYNELTKVKYQT--EGMKRPvFLSSEDKEEIVNLLFK--KER-KVTV 561 AHY15608 487 SARAFIERMTNEDTYLPEEKVLPKHSPLYEMFMVYNELTKVKYQT--EGMKRPvFLSSEDKEEIVNLLFK--KER-KVTV 561 AHY17476 487 SARAFIERMTNEDTYLPEEKVLPKHSPLYEMFMVYNELTKVKYQT--EGMKRPvFLSSEDKEEIVNLLFK--KER-KVTV 561 ESR09100 -------------------------------------------------------------------------------- AGM98575 487 SARAFIERMTNEDTYLPEEKVLPKHSPLYEMFMVYNELTKVKYQT--EGMKRPvFLSSEDKEEIVNLLFK--KER-KVTV 561 ALF27331 487 SAEAFINRMTNYDLYLPNQKVLPRHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIEDGVEK--VYR-KVTK 560 WP_018372492 500 SASRFIERMTLHDLYLPDEKVLPRHSLIYEKYTVFNELTKVRETP--EGGKEV-YESKTDKENIFDSLEK--RYR-KVTK 573 WP_045618028 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKQQIVTQLFK--EKR-KVTE 562 WP_045635197 487 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--ENR-KVTE 561 WP_002263549 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIEDGVEK--VYR-KVTK 560 WP_002263887 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELIKVKYKT--EQGKTA-FFDANMKQEIEDGVEK--VYR-KVTK 560 WP_002264920 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIEDGVEK--VYR-KVTK 560 WP_002269043 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIEDGVEK--VYR-KVTK 560 WP_002269448 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002271977 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002272766 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002273241 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002275430 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002276448 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002277050 487 SAQAFIEHMTNNDLYLPNEKVLPKHSPLYEKYTVYNELTKIKYVT--EIGEAK-FFDANLKQEIFDGLFK--HER-KVTK 560 WP_002277364 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002279025 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGETA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002279859 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002280230 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002281696 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002282247 487 SAQAFIEHMTNNDLYLPNEKVLPKHSPLYEKYTVYNELTKIKYVT--EIGEAK-FFDANLKQEIFDGLFK--HER-KVTK 560 WP_002282906 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002283846 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002287255 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002288990 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002289641 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002290427 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002295753 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002296423 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002304487 497 SAEKFITRMTLNDLYLPEEKVLPKHSLLYETFTVYNELTKVKYVN--EQGEAK-FFDANMKQEIFDHVFK--ENR-KVTK 570 WP_002305844 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002307203 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002310390 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_002352408 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_012997688 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_014677909 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019312892 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019313659 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019314093 487 SVEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019315370 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019803776 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_019805234 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_024783594 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_024784288 487 SAQAFIEHMTNNDLYLPNEKVLPKHSPLYEKYTVYNELTKIKYVT--EIGEAK-FFDANLKQEIFDGLFK--HER-KVTK 560 WP_024784666 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_024784894 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_024786433 487 SAQAFIEHMTNNDLYLPNEKVLPKHSPLYEKYTVYNELTKIKYVT--EIGEAK-FFDANLKQEIFDGLFK--HER-KVTK 560 WP_049473442 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_049474547 487 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 560 EMC03581 480 SAEAFINRMTNYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKT--EQGKTA-FFDANMKQEIFDGVFK--VYR-KVTK 553 WP_000428612 488 SAESFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSRQKKDIFYTLFKaeDKR-KVTE 564 WP_000428613 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVTQLFK--EKR-KVTE 562 WP_049523028 487 SAEDFIHRMTNYDLYLPEEKVLPKHSLLYETFTVYNELTKVKYIA--EGMKDYqFLDSGQKKQIVNQLFK--EKR-KVTE 561 WP_003107102 456 SAKVFIERMTNFDTYLPEEKVLPKHSLLYEMFTVYNELTKVKYQA--EGMRKPeFLSSEEKIEIVSNLFK--TER-KVTV 530 WP_054279288 489 SAQAFIERMTNFDEYLPQEKVLPKHSLTYEYFTVYNELTKVKYVT--EGMTKPeFLSAGQKEQIVELLFK--KYR-KVTV 563 WP_049531101 488 SAEAFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKKIINQLFK--EKR-KVTE 562 WP_049538452 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--EKR-KVTE 562 WP_049549711 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--EKR-KVTE 562 WP_007896501 490 SAQAFIEGMTNYDTYLPEEKVLPKHSPLYEMFTVYNELTKVKYIA--ENMTKPlYLSAEQKEATIDHLFK--QTR-KVTV 564 EFR44625 442 SAQAFIEGMTNYDTYLPEEKVLPKHSPLYEMFTVYNELTKVKYIA--ENMTKPlYLSAEQKEATIDHLFK--QTR-KVTV 516 WP_002897477 487 SAEDFIHRMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--EKR-KVTE 561 WP_002906454 487 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVNQLFK--DKR-KVTE 561 WP_009729476 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFLDSGQKKQIVTQLFK--EKR-KVTE 562 CQR24647 488 SAQAFIERMTNNDLYLPDQKVLPKHSLLYQKFAVYNELTKIKYVT--ETGEAR-LFDVFLKKEIFDGLFK--KER-KVTK 561 WP_000066813 488 SAEDFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLTRYqFLDKKQKKDIFDTFFKaeNKR-KVTE 564 WP_009754323 488 SAESFINKMTNYDLYLPEEKVLPKHSLLYETFAVYNELTKVKFIA--EGLRDYqFFDSGQKKQIVNQLFK--EKR-KVTE 562 WP_044674937 487 SAENFITRMTNYDQYLPDQKVLPKHSLLYEKFAVYNELTKVKFIA--EGMRDYqFLDSGQKKDIVKTLFK--TKR-KVTA 561 WP_044676715 487 SAENFITRMTNYDQYLPDQKVLPKHSLLYEKFAVYNELTKVRYVT--EQGKSF-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_044680361 487 SAENFITRMTNYDQYLPDQKVLPKHSLLYEKFAVYNELTKVRYVT--EQGKSF-FFDANMKQEIFDGVFK--VYR-KVTK 560 WP_044681799 487 SAENFITRMTNYDQYLPDQKVLPKHSLLYEKFAVYNELTKVKFIA--EGMRDYqFLDSGQKKDIVKTLFK--TKR-KVTA 561 WP_049533112 487 SAEKFITRMTLNDLYLPEEKVLPKHSLLYETFTVYNELTKVKYVN--EQGEAK-FFDANMKQEIFDHVEK--ENR-KVTK 560 WP_029090905 472 SSEAFIKRMTNKCTYLIHEDVIPKHSFSYAKFEVLNELNKIRLDG------KP--IDIPLKKRIFEGLFL---EKtKVTQ 540 WP_006506696 499 TAEGFIKRMRSYCTYFPDEEVLPKNSLIVSKYEVYNELNKIRVDD--------kLLEVDVKNDIYNELFM--KNK-TVTE 567 AIT42264 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_034440723 494 SAELFIENLTSRDTYLPDEPVLPKRSLIYQKFTIFNELTKISYID--ERGILQ-NFSSREKIAIFNDLFK---NKsKVTK 567 AKQ21048 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 WP_004636532 489 SAEKFIERMTNMDTYLLEEKVLPKRSLLYQTFEVYNELTKVRYTN--EQGKTE-KLNRQQKAEIIETLFK-qKNR--VRE 562 WP_002364836 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_016631044 447 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 520 EMS75795 232 SAMKFIQRMINYDTYLPTEKVLPKHSILYQKYTIFNELTKVAYKD--ERGIKH-QFSSKEKREIFKELFQ--KQR-KVTV 305 WP_002373311 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_002378009 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_002407324 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_002413717 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_010775580 498 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 571 WP_010818269 496 SATAFIERMTNEDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_010824395 496 SATAFIERMINFDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_016622645 496 SATAFIERMINFDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_033624816 496 SATAFIERMINFDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_033625576 496 SATAFIERMINFDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_033789179 496 SATAFIERMINFDTYLPSEKVLPKHSLLYEKFMVFNELTKISYTD--DRGIKA-NFSGKEKEKIFDYLFK--TRR-KVKK 569 WP_002310644 493 SAVRFIERMINTDMYMPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 566 WP_002312694 494 SAVRFIERMINTDMYMPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_002314015 494 SAVRFIERMNNTDMYIPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_002320716 494 SAVRFIERMINTDMYIPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_002330729 493 SAVRFIERMINTDMYIPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 566 WP_002335161 494 SAVRFIERMINTDMYMPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_002345439 494 SAVRFIERMINTDMYIPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_034867970 487 SAMRFIQRMTKQDTYLPTEKVLPKNSLLYQKYMIFNELTKVSYKD--ERGVKQ-YFSGDEKQQIFKQLFQ--KERgKITV 561 WP_047937432 494 SAVRFIERMINTDMYMPHNKVLPKNSLLYQKFSIYNELTKVRYQD--ERGQMN-YFSSIEKKEIFHELFE--KNR-KVIK 567 WP_010720994 487 SAMRFIQRMTKQDTYLPTEKVLPKNSLFYQKYMIFNELTKVSYKD--ERGVKQ-YFSGDEKQQIFKQLFQ--KERgKITV 561 WP_010737004 487 SAMRFIQRMTKQDTYLPTEKVLPKNSLLYQKYMIFNELTKVSYKD--ERGVKQ-YFSGDEKQQIFKQLFQ--KERgKITV 561 WP_034700478 487 SAMRFIQRMTKQDTYLPTEKVLPKNSLLYQKYMIFNELTKVSYKD--ERGVKQ-YFSGDEKQQIFKQLFQ--KERgKITV 561 WP_007209003 491 SAVAFIERMTIKDIYL-NENVLPRHSLIYEKFTVFNELTKVLYAD--DRGVFQ-RFSAEEKEDIFEKLFK--SER-KVIK 563 WP_023519017 486 SAIEFIERMINQDTYLPKEKVLPKQSLIYQRFMIFNELTKVSYTD--ERGKSH-YFSSEQKRKIFNELFK--QHP-RVIE 559 WP_010770040 490 SATKFIERMINFDTYLPTEKVLPKHSMIYEKYMVYNELTKVSYVD--ERGMNQ-RFSGEEKKQIVEELFK--QSR-KVIK 563 WP_048604708 486 SAELFIERMINFDLYLPSEKVLPKHSMLYEKYTVYNELTKVTYKD--EQGKVQ-NFSSEEKERIFIDLFK--QHR-KVIK 559 WP_010750235 487 SATKFIQRMINYDTYLPTEKVLPKYSMLYQKYTIFNELTKVAYKD--DRGIKH-QFSSEEKLRIFQELFK--KQR-RVIK 560 AII16583 526 SAQSFIERMINFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaFLSGEQKKAIVDLLFK--TNR-KVIV 600 WP_029073316 507 TADEFIKRMRNFCTYFPDEPVLAKNSLIVSKYEVLNEINKLRIND--------hLIKRDIKDKMLHTLFM--DHK-SISA 575 WP_031589969 507 TADEFIKRMRNECTYFPDEPVMAKNSLIVSKYEVLNEINKLRIND--------hLIKRDMKDKMLHTLFM--DHK-SISA 575 KDA45870 485 SAEDFIKRMTINDLYLPTEPVLPKHSLLYERYTIFNELAGVRYVT--ENGEAK-YEDAQTKRSIFE-LFKl--DR-KVSE 557 WP_039099354 510 SANEFIKRMITIDTYLLAEDVLPKQSLIYQRFEVLNELNGLKIDD--QPITTE------LKQAIFTDLFM---QKtSVTV 578 AKP02966 484 SSNKFIRRMTVIDSYLVGEPVLPKNSLIYQRYEVLNELNNIRITEnlKINPIGsRLIVETKQHIYNELFK--NYK-KITV 560 WP_010991369 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYLVYNELTKVRYIN--DQGKTS-YFSGQEKEQIENDLFK--QKR-KVKK 566 WP_033838504 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYLVYNELTKVRYIN--DQGKTS-YFSGQEKEQIENDLFK--QKR-KVKK 566 EHN60060 496 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYLVYNELTKVRYIN--DQGKTS-YFSGQEKEQIENDLFK--QKR-KVKK 569 EFR89594 262 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYLVYNELTKVRYIN--DQGKTS-YFSGQEKEQIENDLFK--QKR-KVKK 335 WP_038409211 493 SAIDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKTH-HFSGQEKQQIFNGLFK--QQR-KVKK 566 EFR95520 112 SAIDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKTH-HFSGQEKQQIFNGLFK--QQR-KVKK 185 WP_003723650 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKIN-YFSGREKQQVFNDLFK--QKR-KVKK 566 WP_003727705 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKIN-YFSGREKQQIENDLFK--QKR-KVKK 566 WP_003730785 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKIN-YFSGREKQQIENDLFK--QKR-KVKK 566 WP_003733029 493 SAVDFIEKMINKDTYLPKENVLPKHSLCYQKYMVYNELTKVRYID--DQGKIN-YFSGQEKQQIENDLFK--QKR-KVKK 566 WP_003739838 493 SAVDFIEKMINKDTYLPKENVLPKHSLYYQKYMVYNELTKVRYID--DQGKIN-YFSGQEKQQIENDYFK--QKR-KVSK 566 WP_014601172 493 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKVRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 566 WP_023548323 493 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 566 WP_031665337 493 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKVRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 566 WP_031669209 493 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKVRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 566 WP_033920898 493 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 566 AKI42028 496 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKVRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 569 AKI50529 496 SAVDFIEKMTNKDTYLPKENVLPKHSLCYQKYMVYNELTKIRYID--DQGKTN-YFSGQEKQQIENDLEK--QKR-KVKK 569 EFR83390 1 ---------------------------------------------------------------IFNDLFK--QKR-KVKK 14 WP_046323366 493 SAIDFIEKMTNKDTYLPKENVLPKHSMCYQKYMVYNELTKIRYTD--DQGKTH-YESGQEKQQIENDLEK--QKR-KVKK 566 AKE81011 503 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 577 CUO82355 503 TAEGFIKRMRSYCTYFPDEEVLPKNSLIVSKYEVYNELNKIRVDD--------kLLEVDVKNDIYNELFM--KNK-TVTE 571 WP_033162887 504 TAEGFIERMKNTGTYFPDEPVMAKNSLTVSKFEVLNELNKIRING--------kLIAVETKKELLSDLFM--KNK-TITD 572 AGZ01981 520 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 594 AKA60242 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 AKS40380 487 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 561 4UN5_B 491 SAQSFIERMTNEDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVT--EGMRKPaELSGEQKKAIVDLLFK--TNR-KVTV 565 WP_010922251 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_039695303 564 EKLLNYLNKE--FPEYRIKDLIGLDKEnkSFNASLGTYHDLKKIL-DK AFLDDKVNEEVIEDIIKTLTLFEDKDMIH 637 WP_045635197 562 KDIIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDAKNEAILENIVHTLTIFEDREMIK 632 5AXW_A 300 KQIAKEILVNe--EDIKGYRVISIGKPe---FTNLKVYHDIKDITARK ------ENAELLDQIAKILTIYQSSEDIQ 368 WP_009880683 246 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 317 WP_010922251 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_011054416 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_011284745 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_011285506 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_011527619 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_012560673 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_014407541 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGAYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDRGMIE 633 WP_020905136 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_023080005 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNTSLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDKEMIE 633 WP_023610282 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNTSLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDKEMIE 633 WP_030125963 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_030126706 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_031488318 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_032460140 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_032461047 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_032462016 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_032462936 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDKEMIE 633 WP_032464890 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_033888930 387 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 458 WP_038431314 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_038432938 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNTSLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDKEMIE 633 WP_038434062 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 BAQ51233 473 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 544 KGE60162 ------------------------------------------------ ----------------------------- KGE60856 ------------------------------------------------ ----------------------------- WP_002989955 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_003030002 561 DKLLNYLNKE--FEEFRIVNLIGLDKEnkAFNSSLGTYHDLRKIL-DK SFLDDKANEKTIEDIIQTLTLFEDREMIR 634 WP_003065552 564 EKLLNYLNKE--FPEYRIKDLIGLDKEnkSFNASLGTYHDLKKIL-DK AFLDDKVNEEVIEDIIKTLTLFEDKDMIH 637 WP_001040076 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIK 632 WP_001040078 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040080 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040081 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040083 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIIQTLTLFEDREMIK 635 WP_001040085 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040087 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040088 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040089 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040090 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040091 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040092 562 KQLLDFLAKE--FEEFRIVDVTGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTITLFEDREMIK 635 WP_001040094 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040095 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040096 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040097 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040098 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040099 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040100 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_001040104 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040105 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040106 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040107 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040108 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040109 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_001040110 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_015058523 562 KQLLDFLAKE--FEEFRIVDVTGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTITLFEDREMIK 635 WP_017643650 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_017647151 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_017648376 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_017649527 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_017771611 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_017771984 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 CFQ25032 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 CFV16040 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 KLJ37842 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 KLJ72361 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 KLL20707 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 KLL42645 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_047207273 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_047209694 563 KDIISFLNK---VDGYEGIAIKGIEKQ---FNASLSTYHDLKKIL-GK DFLDNIDNELILEDIVQTLTLFEDREMIR 632 WP_050198062 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_050201642 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_050204027 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_050881965 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_050886065 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 AHN30376 562 KQLLDFLAKE--FEEFRIVDVTGLDKEnkAFNASLGTYHDLKKIL-DK DFLDNPDNESILEDIVQTITLFEDREMIK 635 EAO78426 562 KKLLDFLAKE--YEEFRIVDVIGLDKEnkAFNASLGTYHDLEKIL-DK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 CCW42055 562 KQLLDFLAKE--FEEFRIVDVTGLDKEnkAFNASLGTYHDLEKIL-GK DFLDNPDNESILEDIVQTLTLFEDREMIK 635 WP_003041502 561 DKLLNYLNKE--FEEFRIVNLTGLDKEnkVENSSLGTYHDLRKIL-NK SFLDNKENAQIIEDIIQTLTLFEDREMIR 634 WP_037593752 562 DKLLNYLNKE--FEEFRIVNLTGLDKEnkAENSSLGTYHDLRKIL-DK SFLDDKANEKTIEDIIQTLTLFEDREMIR 635 WP_049516684 562 DKLLNYLNKE--FEEFRIVNLTGLDKEnkAFNASLGTYHDLRKIL-DK SFLDDKVNEKIIEDIIQTLTLFEDREMIR 635 GAD46167 561 DKLLNYLNKE--FEEFRIVNLTGLDKEnkAENSSLGTYHDLRKIL-DK SFLDDKANEKTIEDIIQTLTLFEDREMIR 634 WP_018363470 562 EKLLNYLDKE--FPEYRIQDLVGLDKEnkSFNASLGTYHDLKKIL-DK SFLDDKVNEEVIEDIIKTLTLFEDREMIQ 635 WP_003043819 572 KQLKEDYFKK--IECFDSVEIIGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 643 WP_006269658 561 DKLLNYLNKE--FEEFRIVNLTGLDKEnkAENSSLGTYHDLRKIL-DK SFLDDKANEKTIEDIIQTLTLFEDREMIR 634 WP_048800889 561 DKLLNYLDKE--FDEFRIVDLTGLDKEnkAFNASLGTYHDLRKIL-DK SFLDDKANEKTIEDIIQTLTLFEDREMIR 634 WP_012767106 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_014612333 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_015017095 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_015057649 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_048327215 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_049519324 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDKEMIE 633 WP_012515931 562 KQLKENYFKK--IECWDSVEITGVEDS---FNASLGTYHDLLKIIQDK DFLDNPDNQKIIEDIILTLTLFEDKKMIS 633 WP_021320964 562 KQLKENYFKK--IECWDSVEITGVEDS---FNASLGTYHDLLKIIQDK DFLDNPDNQKIIEDIILTLTLFEDKKMIS 633 WP_037581760 562 KQLKENYFKK--IECWDSVEITGVEDS---FNASLGTYHDLLKIIQDK DFLDNPDNQKIIEDIILTLTLFEDKKMIS 633 WP_004232481 561 AKLLSYLNNE--FEEFRINDLIGLDKDskSFNASLGTYHDLKKIL-DK SFLDDKTNEQIIEDIVLTLTLFEDRDMIH 634 WP_009854540 562 EKLLNYLNKE--FPEYRIKDLIGLDKEnkSFNASLGTYHDLKKIL-DK AFLDDKVNEEVIEDIIKTLTLFEDKDMIH 635 WP_012962174 562 DKFLNYLNKE--FPEYRIQDLIGLDKEnkSFNASLGTYHDLKKIL-DK SFLDDKTNETIIEDIIQTLTLFEDRDMIR 635 WP_039695303 564 EKLLNYLNKE--FPEYRIKDLIGLDKEnkSFNASLGTYHDLKKIL-DK AFLDDKVNEEVIEDIIKTLTLFEDKDMIH 637 WP_014334983 561 AKLLSYLNNE--FEEFRINDLIGLDKDskSFNASLGTYHDLKKIL-DK SELDDKINGQIIEDIVLILTLFEDRDMIH 634 WP_003099269 562 KQLKEEYESK--MKCFHTVTILGVEDR---FNASLGTYHDLLKIFKDK AFLDDEANQDILEEIVWTLTLFEDQAMIE 633 AHY15608 562 KQLKEEYESK--MKCFHTVTILGVEDR---FNASLGTYHDLLKIFKDK AFLDDEANQDILEEIVWTLTLFEDQAMIE 633 AHY17476 562 KQLKEEYESK--MKCFHTVTILGVEDR---FNASLGTYHDLLKIFKDK AFLDDEANQDILEEIVWTLTLFEDQAMIE 633 ESR09100 ------------------------------------------------ ----------------------------- AGM98575 562 KQLKEEYESK--MKCFHTVTILGVEDR---FNASLGTYHDLLKIFKDK AFLDDEANQDILEEIVWTLTLFEDQAMIE 633 ALF27331 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_018372492 574 RKLKDFIEKElgYGYIDIDNIKGVEEQ---FNASYTTYQDLLKIIGDK EFLDNEENKDLLEEIIYILTVFEDRKMIE 647 WP_045618028 563 KDIIQYLHN---VDSYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDSKNEAILENIVHTLTIFEDREMIK 633 WP_045635197 562 KDIIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDAKNEAILENIVHTLTIFEDREMIK 632 WP_002263549 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002263887 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002264920 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002269043 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002269448 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002271977 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002272766 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002273241 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002275430 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002276448 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002277050 561 KKLRTFLDKN--FDEFRIVDIQGLDKEteTENASYATYQDLLKVIKDK VFMDNPENAEILENIVLTLTLFEDREMIK 635 WP_002277364 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002279025 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002279859 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002280230 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002281696 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002282247 561 KKLRTFLDKN--FDEFRIVDIQGLDKEteTENASYATYQDLLKVIKDK VFMDNPENAEILENIVLTLTLFEDREMIK 635 WP_002282906 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002283846 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002287255 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002288990 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002289641 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002290427 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002295753 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002296423 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002304487 571 DKLLNYLNKE--FEEFRIVNLTGLDKEnkVENSSLGTYHDLRKIL-NK SFLDNKENEQIIEDIIQTLTLFEDREMIR 644 WP_002305844 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002307203 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002310390 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_002352408 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_012997688 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_014677909 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019312892 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019313659 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019314093 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019315370 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019803776 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_019805234 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_024783594 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_024784288 561 KKLRTFLDKN--FDEFRIVDIQGLDKEteTENASYATYQDLLKVIKDK VFMDNPENAEILENIVLTLTLFEDREMIK 635 WP_024784666 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGTYHDLRKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_024784894 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGTYHDLCKIL-DK DELDNSKNEKILEDIVLILTLFEDREMIR 634 WP_024786433 561 KKLRTFLDKN--FDEFRIVDIQGLDKEteTENASYATYQDLLKVIKDK VFMDNPENAEILENIVLTLTLFEDREMIK 635 WP_049473442 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGIYHDLCKIL-DK DFLDNSKNEKILEDIVLTLTLFEDREMIR 634 WP_049474547 561 DKLMDFLEKE--FDEFRIVDLTGLDKEnkVFNASYGIYHDLCKIL-DK DFLDNSKNEKILEDIVLTLTLFEDREMIR 634 EMC03581 554 DKLMDFLEKE--FDEFRIVDLTGLDKEnkAFNASYGIYHDLRKIL-DK DFLDNSKNEKILEDIVLTLTLFEDREMIR 627 WP_000428612 565 KDIIQYLHT---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDPNNEEILENIVHTLTIFEDREMIK 635 WP_000428613 563 KDIIQFLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDSKNEEILENIVHTLTIFEDREMIK 633 WP_049523028 562 KDIIHYLHN---VDGYDGIELKGIEKH---FNSSLSTYHDLLKIIKDK EFMDDPKNEEIFENIVHTLTIFEDRVMIK 632 WP_003107102 531 KQLKENYFNK--IRCLDSITISGVEDK---FNASLGTYHDLLNIIKNQ KILDDEQNQDSLEDIVLTLTLFEDEKMIA 602 WP_054279288 564 KQLKEDFFSK--IECFDTVDISGVEDK---FNASLGTYHDLLKIIKDK AFLDNSENENIIEDIILTLTLFEDKEMIA 635 WP_049531101 563 KDLIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK RFMDEPKNQEILENIVHTLTIFEDREMIK 633 WP_049538452 563 KDIIQYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDSKNEEILENIVHTLTIFEDREMIK 633 WP_049549711 563 KDIIHYLHT---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDDSKNEAILENIVHTLTIFEDREMIK 633 WP_007896501 565 KDLKEKYFSQ--IEGLENVDVTGVEGA---FNASLGTYNDLLKIIKDK AFLDDEANAEILEEIVLILTLFQDEKLIE 636 EFR44625 517 KDLKEKYFSQ--IEGLENVDVTGVEGA---FNASLGTYNDLLKIIKDK AFLDDEANAEILEEIVLILTLFQDEKLIE 588 WP_002897477 562 KDIIHYLHN---VDGYDGIELKGIEKQ---FNANLSTYHDLLKITKDK EFMDDPKNEEILENIVHTLTIFEDREMIK 632 WP_002906454 562 KDIIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDNPKNGEILENIIHTLTIFEDREMIK 632 WP_009729476 563 KDIIQFLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK AFMDDAKNEAILENIVHTLTIFEDREMIK 633 CQR24647 562 KKILNFLDKN--FDEFRITDIQGLDNEtgNFNASYGIYHDLLKIIGDK EFMDSSDNVDVLEDIVLSLTLFEDREMIK 636 WP_000066813 565 KDIIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK AFMDDSKNEEILENIIHTLTIFEDREMIK 635 WP_009754323 563 KDIIHYLHN---VDGYDGIELKGIEKQ---FNASLSTYHDLLKIIKDK EFMDNHKNQEILENIVHTLTIFEDREMIK 633 WP_044674937 562 KDIKAYL-EN--SNGYAGVELKGLEEQ---FNASLPTYHDLLKILRDK AFIDAEENQEILEDIVLTLTLFEDREMIR 632 WP_044676715 561 EKLMDFLGKE--FDEFRIVDLLGLDKDnkSFNASLGTYHDLKKIV-SK DLLDNPENEDILENVVLTLTLFEDREMIR 634 WP_044680361 561 EKLMDFLGKE--FDEFRIVDLLGLDKDnkSFNASLGTYHDLKKIV-SK DLLDNPENEDILENVVLTLTLFEDREMIR 634 WP_044681799 562 KDIKAYL-EN--SNGYAGVELKGLEEQ---FNASLPTYHDLLKILRDK AFIDAEENQEILEDIVLTLTLFEDREMIR 632 WP_049533112 561 DKLLNYLGKE--FDEFRIVDLTGLDKEnkVFNSSLGTYHDLRKIL-DK SFLDNKENEQIIEDIIQTLTLFEDREMIR 634 WP_029090905 541 TSLKKWLAEH---EHMTVSVVQGTQKEt-EFATSLQAFHRFVKIF-DR ETVSNPANEEMFEKIIYWSTVFEDKKIMR 612 WP_006506696 568 KKLKNWLVNNgcCS--KDAEIKGFQKEn-QESTSLIPWIDETNIFGKI ----DQSNFDLIENIIYDLTVFEDKKIMK 637 AIT42264 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_034440723 568 NQLVKYIENK---EQIIAPEIKGIEDS---FNSNYSTYIDLSKIPDMK --LLEKDEDEILEEIIKILTIFEDRKMRK 637 AKQ21048 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 633 WP_004636532 563 KDIANYLEQ---YGYVDGTDIKGVEDK---FNASLSTYNDLAKIDGAK AYLDDPEYADVWEDIIKILTIFEDKAMRK 633 WP_002364836 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_016631044 521 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 592 EMS75795 306 KKLQQFLSAN--YN-IEDAEILGVDKA---FNSSYATYHDFLDLAKPN ELLEQPEMNAMFEDIVKILTIFEDREMIR 381 WP_002373311 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_002378009 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_002407324 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_002413717 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_010775580 572 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 643 WP_010818269 570 KDIIQFYRNE--YN-TEIVILSGLEED--QFNASFSTYQDLLKCGLIR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_010824395 570 KDIIQFYRNE--YN-TEIVTLSGLEED--QFNASFSTYQDLLKCGLTR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_016622645 570 KDIIQFYRNE--YN-TEIVTLSGLEED--QFNASFSTYQDLLKCGLTR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_033624816 570 KDIIQFYRNE--YN-TEIVTLSGLEED--QFNASFSTYQDLLKCGLTR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_033625576 570 KDIIQFYRNE--YN-TEIVTLSGLEED--QFNASFSTYQDLLKCGLTR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_033789179 570 KDIIQFYRNE--YN-TEIVTLSGLEED--QFNASFSTYQDLLKCGLTR AELDHPDNAEKLEDIIKILTIFEDRQRIR 641 WP_002310644 567 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 641 WP_002312694 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_002314015 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_002320716 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_002330729 567 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 641 WP_002335161 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_002345439 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_034867970 562 KKLQNFLYTH--YH-IENAQIFGIEKA---FNASYSTYHDFMKLAKTN EWLEQPEMEPIFEDIVKILTIFEDRQMIK 637 WP_047937432 568 KDLQEFLYLK--YD-IKHAELSGIEKA---FNASYTTYHDFLTMSENK QWLEDPELASMFEEIIKILTVFEDREMIK 642 WP_010720994 562 KKLQNFLYTH--YH-IENAQIFGIEKA---FNASYSTYHDFMKLAKTN EWLEQPEMEPIFEDIVKILTIFEDRQMIK 637 WP_010737004 562 KKLQNFLYTH--YH-IENAQIFGIEKA---FNASYSTYHDFMKLAKTN EWLEQPEMEPIFEDIVKILTIFEDRQMIK 637 WP_034700478 562 KKLQNFLYTH--YH-IENAQIFGIEKA---FNASYSTYHDFMKLAKTN EWLEQPEMEPIFEDIVKILTIFEDRQMIK 637 WP_007209003 564 KKLENYLRIEl---SISSPSVKGIEEQ---FNANFGTYLDLKKFDELH PYLDDEKYQDTLEEVIKVLTVFEDRSMIQ 634 WP_023519017 560 KQLRKFLELN--EQ-IDSTEIKGIETS---FNASYSTYHDLLKLS--- ILLDDPDMITMFEEIIKILTIFEDREMIR 631 WP_010770040 564 KLLEKFLSNE--FG-LVDVAIKGIE-T--SFNAGYGTYHDFLKIGITR EQLDKEENSETLEEIVKILTVFEDRKMIR 634 WP_048604708 560 KDLSNFLRNE--YN-LDDVIIDGIE-N--KFNASFNTYHDFLKLKIDP KVLDDPANEPMFEEIVKILTIFEDRKMLR 630 WP_010750235 561 KKLQHFLSAN--YN-IEDAEILGVDKV---FNSSYATYHDFLELAKPY ELLEQPEMEEMFEDIVKIITIFEDREMVR 636 AII16583 601 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DFLDNEENEDILEDIVLTLTLFEDREMIE 672 WP_029073316 576 NAMKKWLVKNgyFSNIDDIKIEGFQKEn-ACSTSLIPWIDETKIFGEI ----NNSNYELIEKIIYDVTVFEDKKILR 647 WP_031589969 576 NAMKKWLVKNgyFSNIDDIKIEGFQKEn-ACSTSLIPWIDETKIFGKI ----NESNYDFIEKIIYDVTVFEDKKILR 647 KDA45870 558 KMVIKHLKVV--MPAIRIQALKGLDNGk--FNASYGTYKDLVDMGVAP ELLNDEVNSEKWEDIIKTLTIFEGRKLIK 630 WP_039099354 579 KNIQDYLVSEk--RYASRPAITGLSDEnk-FNSRLSTYHDLKTIVGDA --VDDVDKQADLEKCIEWSTIFEDGKIYS 650 AKP02966 561 KKLIKWLIAQg---YYKNPILIGLSQKd-EFNSTLITYLDMKKIFGSS -FMENNKNYNQIEELIEWLTIFEDKQILN 632 WP_010991369 567 KDLELFLRNM--SH-VESPTIEGLE-D--SFNSSYSTYHDLLKVGIKQ EILDNPVNTEMLENIVKILTVFEDKRMIK 637 WP_033838504 567 KDLELFLRNM--SH-VESPTIEGLE-D--SFNSSYSTYHDLLKVGIKQ EILDNPVNTEMLENIVKILTVFEDKRMIK 637 EHN60060 570 KDLELFLRNM--SH-VESPTIEGLE-D--SFNSSYSTYHDLLKVGIKQ EILDNPVNTEMLENIVKILTVFEDKRMIK 640 EFR89594 336 KDLELFLRNM--SH-VESPTIEGLE-D--SFNSSYSTYHDLLKVGIKQ EILDNPVNTEMLENIVKILTVFEDKRMIK 406 WP_038409211 567 KDLERFLYTI--NH-IESPTIEGVE-D--AFNSSFATYHDLQKGGVTQ EILDNPLNADMLEEIVKILTVFEDKRMIK 637 EFR95520 186 KDLERFLYTI--NH-IESPTIEGVE-D--AFNSSFATYHDLQKGGVTQ EILDNPLNADMLEEIVKILTVFEDKRMIK 256 WP_003723650 567 KDLELFLRNI--NH-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 WP_003727705 567 KDLELFLRNI--NH-IESPTIEGLE-D--SFNASYATYHDLLKVGLKQ EILDNPLNTEILEDIVKILTVFEDKRMIK 637 WP_003730785 567 KDLELFLRNI--NH-IESPTIEGLE-D--SFNASYATYHDLLKVGLKQ EILDNPLNTEILEDIVKILTVFEDKRMIK 637 WP_003733029 567 KDLELFLRNI--NQ-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKRMIK 637 WP_003739838 567 KDLEQFLRNM--SH-IESPTIEGLE-D--SENSSYATYHDLLKVGIKQ EVLENPLNTEMLEDIVKILTVFEDKRMIK 637 WP_014601172 567 KDLELFLRNI--NH-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 WP_023548323 567 KDLELFLRNI--NH-VESPTIEGLE-D--SFNASYATYHDLMKVGIKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 WP_031665337 567 KDLELFLRNI--NQ-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 WP_031669209 567 KDLELFLRNI--NQ-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 WP_033920898 567 KDLELFLRNI--NH-VESPTIEGLE-D--SFNASYATYHDLMKVGIKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 637 AKI42028 570 KDLELFLRNI--NH-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 640 AKI50529 570 KDLELFLRNI--NH-VESPTIEGLE-D--SFNASYATYHDLMKVGIKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 640 EFR83390 15 KDLELFLRNI--NQ-IESPTIEGLE-D--SFNASYATYHDLLKVGMKQ EILDNPLNTEMLEDIVKILTVFEDKPMIK 85 WP_046323366 567 KDLELFLYNM--NH-VESPTVEGVE-D--AFNSSFTTYHDLQKVGVPQ EILDDPLNTEMLEEIIKILTVFEDKRMIN 637 AKE81011 578 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 649 CUO82355 572 KKLKNWLVNNqcCR--KDAEIKGFQKEn-QESTSLTPWIDETNIFGKI ----DQSNFDLIEKIIYDLTVFEDKKIMK 641 WP_033162887 573 KKLKDWLVTHqyYDINEELKIEGYQKDl-QESTSLAPWIDETKIFGEI ----NASNYQLIEKIIYDISIFEDKKILK 644 AGZ01981 595 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 666 AKA60242 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 633 AKS40380 562 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 633 4UN5_B 566 KQLKEDYFKK--IECFDSVEISGVEDR---FNASLGTYHDLLKIIKDK DELDNEENEDILEDIVLILTLFEDREMIE 637 WP_010922251 634

702 WP_039695303 638 ERLQKYSDIFTANQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDYLI DDG---SANRNFMQLINDDIL 706 WP_045635197 633 QRLAQYDSLFDEKVIKALTR-RHYTGWGKLSAKLINGICDK QTGNTILDYLI DDG---KINRNFMQLINDDGL 701 5AXW_A 369 EELTNLNSELTQEEIEQISN1KGYIGTHNLSLKAINLILDE ---------LW -------TNDNQIAIFNRLKL 426 WP_009880683 318 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 386 WP_010922251 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_011054416 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_011284745 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_011285506 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_011527619 634 ERLKTYAHLFDDKVMKQLKR-RRYTVWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_012560673 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_014407541 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_020905136 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_023080005 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_023610282 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_030125963 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_030126706 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_031488318 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_032460140 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_032461047 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_032462016 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_032462936 634 ERLKKYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_032464890 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_033888930 459 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 527 WP_038431314 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_038432938 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_038434062 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 BAQ51233 545 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 613 KGE60162 ----------------------------------------- ----------- --------------------- KGE60856 ----------------------------------------- ----------- --------------------- WP_002989955 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_003030002 635 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 703 WP_003065552 638 ERLQKYSDIFTADQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDYLI DDG---SANRNFMQLINDDIL 706 WP_001040076 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040078 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040080 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040081 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040083 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040085 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040087 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040088 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040089 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040090 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040091 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040092 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDR ESQKTILDYLI SDG---RANRNFMQLINDDGL 704 WP_001040094 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040095 633 KRLDIYKDFFTESQLKKLYR-RHYTGWERLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040096 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040097 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040098 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040099 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040100 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_001040104 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040105 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_001040106 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_001040107 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_001040108 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_001040109 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_001040110 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_015058523 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDR ESQKTILDYLI SDG---RANRNFMQLINDDGL 704 WP_017643650 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_017647151 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---KSNRNFMQLIHDDGL 704 WP_017648376 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---KSNRNFMQLIHDDGL 704 WP_017649527 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_017771611 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_017771984 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 CFQ25032 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 CFV16040 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 KLJ37842 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 KLJ72361 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 KLL20707 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 718 KLL42645 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_047207273 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_047209694 633 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 701 WP_050198062 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_050201642 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_050204027 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI SDG---RANRNFMQLIHDDGL 704 WP_050881965 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 WP_050886065 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 AHN30376 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDR ESQKIILDYLI SDG---RANRNFMQLINDDGL 704 EAO78426 636 KRLENYKDLFTESQLKKLYR-RHYTGWGRLSAKLINGIRDK ESQKTILDYLI DDG---RSNRNFMQLINDDGL 704 CCW42055 636 KRLDIYKDFFTESQLKKLYR-RHYTGWGRLSAKLINGIRNK ENQKTILDYLI DDG---SANRNFMQLIKDAGL 704 WP_003041502 635 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 703 WP_037593752 636 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 704 WP_049516684 636 QRLQKYSDIFTTQQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 704 GAD46167 635 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 703 WP_018363470 636 QRLQKYSDIFTKQQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDYLI DDG---SANRNFMQLINDDAL 704 WP_003043819 644 ERLKTYAHLFDDKVMKQLKR-RHYTGWGRLSRKMINGIRDK QSGKTILDFLK -DGf---SNRNFMQLIHDDSL 712 WP_006269658 635 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 703 WP_048800889 635 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILEYLV DDG---YANRNFMQLINDDIL 703 WP_012767106 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_014612333 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_015017095 634 ERLKTYAHLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFIQLIHDDSL 702 WP_015057649 634 ERLKKYANLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLINDDSL 702 WP_048327215 634 ERLKTYAHLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFIQLIHDDSL 702 WP_049519324 634 ERLKTYAHLFDDKVMKQLKR-RHYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFIQLIHDDSL 702 WP_012515931 634 KRLDQYAHLFDKVVLNKLER-HHYTGWGRLSGKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDSEL 702 WP_021320964 634 KRLDQYAHLFDKVVLNKLER-HHYTGWGRLSGKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDSEL 702 WP_037581760 634 KRLDQYAHLFDKVVLNKLER-HHYTGWGRLSGKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDSEL 702 WP_004232481 635 ERLQKYSDIFTSQQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDFLI DDG---DANRNFMQLINDDSL 703 WP_009854540 636 ERLQKYSDIFTANQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDYLI DDG---SANRNFMQLINDDIL 704 WP_012962174 636 QRLQKYSDIFTPQQLKKLER-RHYTGWGRLSYKLINGIRNK ENGKSILDYLI DDG---YANRNFMQLISDDTL 704 WP_039695303 638 ERLQKYSDIFTANQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDYLI DDG---SANRNFMQLINDDIL 706 WP_014334983 635 ERLQKYSDFFTSQQLKKLER-RHYTGWGRLSYKLINGIRNK ENNKTILDFLI DDG---HANRNFMQLINDESL 703 WP_003099269 634 RRLVKYADVFEKSVLKKLKK-RHYTGWGRLSQKLINGIKDK QTGKTILGFLK -DGv---ANRNFMQLINDSSL 702 AHY15608 634 RRLVKYADVFEKSVLKKLKK-RHYTGWGRLSQKLINGIKDK QTGKTILGFLK -DGv---ANRNFMQLINDSSL 702 AHY17476 634 RRLVKYADVFEKSVLKKLKK-RHYTGWGRLSQKLINGIKDK QTGKTILGFLK -DGv---ANRNFMQLINDSSL 702 ESR09100 ----------------------------------------- ----------- --------------------- AGM98575 634 RRLVKYADVFEKSVLKKLKK-RHYTGWGRLSQKLINGIKDK QTGKTILGFLK -DGv---ANRNFMQLINDSSL 702 ALF27331 635 KRLENYSDLLTKEQVKNLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_018372492 648 KRLSELNIPFENKIIKKLAR-KKYTGWGNLSRKLIDGIRNR ETNRTILGHLI DDGf---SNRNLMQLINDDGL 716 WP_045618028 634 QRLAHYASIFDEKVIKALTR-RHYTGWGKLSAKLINGIYDK QSKKTILDYLI DDG---EINRNFMQLINDDGL 702 WP_045635197 633 QRLAQYDSLFDEKVIKALTR-RHYTGWGKLSAKLINGICDK QTGNTILDYLI DDG---KINRNFMQLINDDGL 701 WP_002263549 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002263887 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002264920 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002269043 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002269448 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002271977 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002272766 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002273241 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002275430 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTLLDYLI DDG---NSNRNFMQLINDDAL 703 WP_002276448 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002277050 636 QRLAKYADVFDKKVIDQLAR-RHYTGWGRLSAKLLNGIRDK QSCKTIMDYLI DDA---QSNRNLMQLITDDNL 704 WP_002277364 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002279025 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002279859 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002280230 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002281696 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002282247 636 QRLAKYADVFDKKVIDQLAR-RHYTGWGRLSAKLLNGIRDK QSCKTIMDYLI DDA---QSNRNLMQLITDDNL 704 WP_002282906 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002283846 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002287255 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002288990 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002289641 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002290427 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002295753 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002296423 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002304487 645 QRLQKYSDIFTKAQLKKLER-RHYTGWGRLSYKLINGIRDK QSNKTILGYLI DDG---YSNRNFMQLINDDAL 713 WP_002305844 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002307203 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTLLDYLI DDG---NSNRNFMQLINDDAL 703 WP_002310390 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_002352408 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTLLDYLI DDG---NSNRNFMQLINDDAL 703 WP_012997688 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_014677909 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_019312892 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_019313659 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_019314093 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTLLDYLI DDG---NSNRNFMQLINDDAL 703 WP_019315370 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTLLDYLI DDG---NSNRNFMQLINDDAL 703 WP_019803776 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_019805234 635 KRLKNYSDLLTKEQLKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_024783594 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_024784288 636 QRLAKYADVFDKKVIDQLAR-RHYTGWGRLSAKLLNGIRDK QSCKTIMDYLI DDA---QSNRNLMQLITDDNL 704 WP_024784666 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_024784894 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_024786433 636 QRLAKYADVFDKKVIDQLAR-RHYTGWGRLSAKLLNGIRDK QSCKTIMDYLI DDA---QSNRNLMQLITDDNL 704 WP_049473442 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 WP_049474547 635 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 703 EMC03581 628 KRLENYSDLLTKEQVKKLER-RHYTGWGRLSAELIHGIRNK ESRKTILDYLI DDG---NSNRNFMQLINDDAL 696 WP_000428612 636 QRLAQYDSLFDEKVIKALTR-RHYTGWGKLSSKLINGIRDK QTGKTILDYLM DDG---YNNRNFMQLINDDEL 704 WP_000428613 634 QRLAQYDSLFDEKVIKALIR-RHYTGWGKLSAKLIDGICDK QTGNTILDYLI DDG---KNNRNFMQLINDDGL 702 WP_049523028 633 QRLNQYDSIFDEKVIKALTR-RHYTGWGKLSAKLINGIRDK KTSKTILDYLI DDG---YSNRNFMQLINDDGL 701 WP_003107102 603 KRLSKYESIFDPSILKKLKK-RHYTGWGRLSQKLINGIRDK QTGKTILDFLI -DGq---ANRNFMQLINDPSL 671 WP_054279288 636 NRLAVYEDLFDQNVLKQLKR-RHYTGWGRLSKQLINGMRDK HTGKTILDFLK -DGf---INRNFMQLINDDNL 704 WP_049531101 634 QRLAQYASIFDEKVIKTLTR-RHYTGWGKLSAKLINCIRDR KTGKTILDYLI DDG---YNNRNFMQLINDDGL 702 WP_049538452 634 QRLAQYDSIFDEKVIKALTR-RHYTGWGKLSAKLINGIRDK QTGKTILDYLI DDG---YSNRNFMQLINDDGL 702 WP_049549711 634 QRLAQYDSLFDKKVIKALTR-RHYTGWGKLSAKLINGICDK QTGNTILDYLI DDG---EINRNFMQLINDDGL 702 WP_007896501 637 KRLAKYANLFEKSVLKKLRK-RHYRGWGRLSRQLIDGMKDK ASGKTILDFLK -DDf---ANRNFIQLINDSSL 705 EFR44625 589 KRLAKYANLFEKSVLKKLRK-RHYRGWGRLSRQLIDGMKDK ASGKTILDFLK -DDf---ANRNFIQLINDSSL 657 WP_002897477 633 QRLAQYDTLFDEKVIKALTR-RHYTGWGKLSAKLINGIRDK QSGKTILDYLI DDD---KINRNFMQLINDDGL 701 WP_002906454 633 QRLAQYDTLFDEKVIKALTR-RHYTGWGKLSAKLINGIRDK QTGKTILEYLI DDG---DCNRNFMQLINDDGL 701 WP_009729476 634 QRLAQYDSLFDEKVIKALTR-RHYTGWGKLSAKLINGISDK QTGNTILDYLI DDG---EINRNFMQLINDDGL 702 CQR24647 637 QRLLKYEDIFSKKVIANLTR-RHYTGWGRLSAKLINGIKDK HSRKTILDYLI DDG---HSNRNFMQLINDDNL 705 WP_000066813 636 QRLAQYDSLFDEKVIKALTR-RHYTGWGKLSAKLINGIRDK KSGKTILDYLI DDG---EINRNFMQLIHDDGL 704 WP_009754323 634 QRLAQYDSIFDEKVIKALTR-RHYTGWGKLSAKLINGICDK KTGKTILDYLI DDG---YNNRNFMQLINDDGL 702 WP_044674937 633 KRLEKYKDILTEEQRKKLER-RHYTGWGRLSAKLINGILDK VTRKTILGYLI DDG---TSNRNFMQLINDDIL 701 WP_044676715 635 KRLEKYKDVLTEEQRKKLER-RHYTGWGRLSAKLINGIRDK VTRKTILDYLI DDG---TSNRNFMQLINDDIL 703 WP_044680361 635 KRLEKYKDVLTEEQRKKLER-RHYTGWGRLSAKLINGIRDK VTRKTILDYLI DDG---TSNRNFMQLINDDIL 703 WP_044681799 633 KRLEKYKDILTEEQRKKLER-RHYTGWGRLSAKLINGILDK VTRKTILGYLI DDG---TSNRNFMQLINDDIL 701 WP_049533112 635 QRLQKYSDIFTKAQLKKLER-CHYTGWGRLSYKLINGIRNK ENKKTILDYLI DDG---YANRNFMQLINDDAL 703 WP_029090905 613 RKLSEYPQLTEQQQVQLAQV--RFRGWGRLSQRLINRIKTP EDHKLSINEIL ------QTNENFMQIIRNKDY 682 WP_006506696 638 RRLKKKYALPDDKVKQILKL--KYKDWSRLSKKLLDGIVAD SV--TVLDVLE -------SRLNLMEIINDKDL 705 AIT42264 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_034440723 638 RQLMKFKDKLSEKAINQLSK-KHYTGWGQLSEKLINGIRDE QSNKTILDYLI DNGcpkNMNRNFMQLINDDIL 710 AKQ21048 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 WP_004636532 634 KQLQTYSDTLSPEILKKLER-KHYTGWGRFSKKLINGLRDE GSNKTILDYLK DEGssgPINRNFMQLIRDNIL 706 WP_002364836 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILGYLI DDGvskHYNRNFMQLINDSQL 714 WP_016631044 593 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLV DDGvskHYNRNFMQLINDSQL 665 EMS75795 382 TQLKKYQSVLGDGFFKKLVK-KHYTGWGRLSERLINGIRDK KTNKTILDYLI DDDfpyNRNRNFMQLINDDSL 454 WP_002373311 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLV DDGvskHYNRNFMQLINDSQL 714 WP_002378009 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 714 WP_002407324 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLV DDGvskHYNRNFMQLINDSQL 714 WP_002413717 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLV DDGvskHYNRNFMQLINDSQL 714 WP_010775580 644 TQLSTFKGQFSEEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 716 WP_010818269 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILGYLI DDGvskHYNRNFMQLINDSQL 714 WP_010824395 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 714 WP_016622645 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLV DDGvskHYNRNFMQLINDSQL 714 WP_033624816 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 714 WP_033625576 642 TQLSTFKGQFSAEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 714 WP_033789179 642 TQLSTFKGQFSEEVLKKLER-KHYTGWGRLSKKLINGIYDK ESGKTILDYLI DDGvskHYNRNFMQLINDSQL 714 WP_002310644 642 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 714 WP_002312694 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 715 WP_002314015 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 715 WP_002320716 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 715 WP_002330729 642 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 714 WP_002335161 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 715 WP_002345439 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNCMQLINDDSL 715 WP_034867970 638 HQLSKYQEVFGEKLLKEFAR-KHYTGWGRFSAKLIHGIRDR KTNKTILDYLI DDDvpaNRNRNLMQLINDEHL 710 WP_047937432 643 TRLSHHEATLGKHIIKKLIK-KHYTGWGRLSKELIQGIRDK QSNKTILDYLI DDDfphHRNRNFMQLINDDSL 715 WP_010720994 638 HQLSKYQEVFGEKLLKEFAR-KHYTGWGRFSAKLIHGIRDR KTNKTILDYLI DDDvpaNRNRNLMQLINDEHL 710 WP_010737004 638 HQLSKYQEVFGEKLLKEFAR-KHYTGWGRFSAKLIHGIRDR KTNKTILDYLI DDDvpaNRNRNLMQLINDEHL 710 WP_034700478 638 HQLSKYQEVFGEKLLKEFAR-KHYTGWGRFSAKLIHGIRDR KTNKTILDYLI DDDvpaNRNRNLMQLINDEHL 710 WP_007209003 635 NQLEQLPLNLSTKTIKALSR-RKYTGWGRLSARLIDGIHDK NSGKTILDYLI DESdsyIVNRNFMQLINDDHL 707 WP_023519017 632 EQLKPYETVLGLPAIKKLAK-KHYTGWGRLSEKMIQGMREK QSRKTILDYLI DDDfpcNRNRNFMQLINDDHL 704 WP_010770040 635 EQLKKYTYLFDEEVLKKLER-RHYTGWGRLSAKLLIGIKEK RTHKTILDYLI DDGgkqPINRNLMQLINDSDL 707 WP_048604708 631 EQLSKFSDRLSEKTIKDLER-RHYTGWGRLSAKLINGIHDK QSNKTILDYLI DDApkkNINRNFMQLINDNRL 703 WP_010750235 637 TQLKKYQRILGEEIFKKLVK-KKYTGWGRLSKRLINGIRDQ KTNKTILDYLI DDDfpyNRNRNFMQLINDDHL 709 AII16583 673 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 741 WP_029073316 648 RRLKKEYDLDEEKIKKILKL--KYSGWSRLSKKLLSGIKTK RTPETVLEVME -------TNMNLMQVINDEKL 717 WP_031589969 648 RRLKKEYDLDEEKIKKILKL--KYSGWSRLSKKLLSGIKTK RTPETVLEVME -------TNMNLMQVINDEKL 717 KDA45870 631 RRLENYRDFLGEDILRKLSR-KKYTGWGRLSAKLLDGIYDK KTHKTILDCLM EDYs-----QNFMQLINDDTY 698 WP_039099354 651 AKLNEIDWLIDQQRVQLAAK--RYRGWGRLSAKLLTQIVN- ANGQRIMDLLW -------TTDNFMRIVHSE-- 712 AKP02966 633 EKLHSSNYSYTSDQIKKISN-MRYKGWGRLSKKILICITTE TNTPKSLQLSN -DLm-wITNNNFISIISNDKY 706 WP_010991369 638 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLMGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_033838504 638 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLMGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 EHN60060 641 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLMGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 709 EFR89594 407 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLMGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 475 WP_038409211 638 EQLQSFSDVLDGTILKKLER-RHYTGWGRLSAKLLTGIRDK HSHLTILDYLM DDG----LNRNLMQLINDSNL 706 EFR95520 257 EQLQSFSDVLDGTILKKLER-RHYTGWGRLSAKLLTGIRDK HSHLTILDYLM DDG----LNRNLMQLINDSNL 325 WP_003723650 638 EQLQQFSDVLDGGVLKKLER-RHYTGWGRLSAKLLVGIREK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_003727705 638 EQLEQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_003730785 638 EQLEQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_003733029 638 EQLQQFSDVLDGTVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_003739838 638 EQLQQFSDVLDGAVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_014601172 638 EQLQQFSDVLDGGVLKKLER-RHYTGWGRLSAKLLVGIREK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_023548323 638 EQLQQFSDVLDGTVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_031665337 638 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILEYLM DDG----LNRNLMQLINDSNL 706 WP_031669209 638 EQLQQFSDVLDGTVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 WP_033920898 638 EQLQQFSDVLDGTVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 706 AKI42028 641 EQLQQFSDVLDGGVLKKLER-RHYTGWGRLSAKLLVGIREK QSHLTILDYLM DDG----LNRNLMQLINDSNL 709 AKI50529 641 EQLQQFSDVLDGTVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILDYLM DDG----LNRNLMQLINDSNL 709 EFR83390 86 EQLQQFSDVLDGVVLKKLER-RHYTGWGRLSAKLLVGIRDK QSHLTILEYLM DDG----LNRNLMQLINDSNL 154 WP_046323366 638 ERLQEFSNVLDEAVLKKLER-RHYTGWGRLSAKLLIGIRDK ESHLTILDYLM DDK----HNRNLMQLINDSNL 706 AKE81011 650 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 718 CUO82355 642 RRLKKKYALPDDKIKQILKL--KYKDWSRLSKKLLDGIVAD SV--TVLDVLE -------SRLNLMEIINDKEL 709 WP_033162887 645 RRLKKVYQLDDLLVDKILKL--NYTGWSRLSEKLLTGMTAD KA--TVLFVLE -------SNKNLMEIINDEKL 712 AGZ01981 667 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 735 AKA60242 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 AKS40380 634 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 702 4UN5_B 638 ERLKTYAHLFDDKVMKQLKR-RRYTGWGRLSRKLINGIRDK QSGKTILDFLK -DGf---ANRNFMQLIHDDSL 706 WP_010922251 703

777 WP_039695303 707 PFKQIIQKSQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-GNPDNIVIEMARENQ TTNRGRSQS 780 WP_045635197 702 SFKEIIQKAQVIG-KTDD-VKQVVQELSGSPAIKKGILQSIKIVDELVKVMG-HAPESIVIEMARENQ TTARGKKNS 775 5AXW_A 427 VPKKVDLSQQKEI---PT---TLVDDFILSPVVKRSFIQSIKVINAIIKKYG--LPNDIIIELAREKN --------S 487 WP_009880683 387 TFKEDLQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 461 WP_010922251 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_011054416 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_011284745 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_011285506 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_011527619 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_012560673 703 TFKEDLQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_014407541 703 TFKEDIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQTVKIVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_020905136 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_023080005 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQTVKIVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_023610282 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQTVKIVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_030125963 703 TFKEDLQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_030126706 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_031488318 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_032460140 703 TFKEDLQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_032461047 703 TFKEDLQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_032462016 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_032462936 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_032464890 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_033888930 528 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 602 WP_038431314 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_038432938 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQTVKIVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_038434062 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKIVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 BAQ51233 614 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 688 KGE60162 -------------------------------------------------------------------- --------- KGE60856 -------------------------------------------------------------------- --------- WP_002989955 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_003030002 704 SEKEEIARAQIIG-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ MTDKGRRNS 777 WP_003065552 707 PFKQIIQKSQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-DNPDNIVIEMARENQ TTNRGRSQS 780 WP_001040076 702 SFKPIIDKARTGS-HSDN-LKEVIGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040078 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040080 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040081 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040083 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040085 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040087 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040088 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040089 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040090 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVVG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040091 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040092 705 SFKSIISKAQSGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040094 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040095 702 SFKPIIDKARTGS-HLDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040096 702 SFKPIIDKARTGS-HLDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040097 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRL 775 WP_001040098 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040099 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040100 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_001040104 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040105 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_001040106 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_001040107 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_001040108 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_001040109 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_001040110 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_015058523 705 SFKSIISKAQSGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_017643650 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRL 775 WP_017647151 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_017648376 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_017649527 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_017771611 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_017771984 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 CFQ25032 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 CFV16040 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 KLJ37842 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 KLJ72361 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 KLL20707 719 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 792 KLL42645 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNKGRRNT 778 WP_047207273 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_047209694 702 SFKPIIDKARTGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTAKGLSRS 775 WP_050198062 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_050201642 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_050204027 705 SFKPIIDKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQVVVEMARENQ TTNQGRRNT 778 WP_050881965 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 WP_050886065 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 AHN30376 705 SFKSIISKAQSGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 EAO78426 705 SFKSIISKAQAGS-HSDN-LKEVVGELAGSPAIKKGILQSLKIVDELVKVMG-YEPEQIVVEMARENQ TTNQGRRNS 778 CCW42055 705 SFKSIISKAQSGS-HSDN-LKEVVSELAGSPAIKKGILQSLKIVDELVKVMG-YKPEQIVVEMARENQ TTNQGRRNS 778 WP_003041502 704 SFKEEIAKAQIIG-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ TTDRGRRNS 777 WP_037593752 705 SEKEEIARAQIIG-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ TTDKGRRNS 778 WP_049516684 705 SEKEEIARAQIIG-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ TTDKGRRNS 778 GAD46167 704 SEKEEIARAQIIG-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ TTDKGRRNS 777 WP_018363470 705 SFKQIIQEAQVVG-DVDD-IETVVHDLPGSPAIKKGILQSVKIVDELIKVMG-DNPDNIVIEMARENQ TTNRGRSQS 778 WP_003043819 713 TFKEEIEKAQVSG-QGDS-LHEQIADLAGSPAIKKGILQTVKIVDELVKVMG-HKPENIVIEMARENQ TTTKGLQQS 786 WP_006269658 704 SEKEEIARAQIID-DVDD-IANVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPANIIIEMARENQ TTDKGRRNS 777 WP_048800889 704 PFKQIIKDAQAID-DVDD-IELIVHDLPGSPAIKKGILQSIKIVDELVKVMG-YNPDNIVIEMARENQ TTTKGRRNS 777 WP_012767106 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_014612333 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_015017095 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_015057649 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_048327215 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_049519324 703 TFKEAIQKAQVSG-QGHS-LHEQIANLAGSPAIKKGILQSVKVVDELVKVMG-HKPENIVIEMARENQ TTQKGQKNS 776 WP_012515931 703 SFIDEIAKAQVIG-KTEY-SKDLVGNLAGSPAIKKGISQTIKIVDELVKIMG-YLPQQIVIEMARENQ TTAQGIKNA 776 WP_021320964 703 SFIDEIAKAQVIG-KTEY-SKDLVGNLASSPAIKKGISQTIKIVDELVKIMG-YLPQQIVIEMARENQ TTAQGIKNA 776 WP_037581760 703 SFIDEIAKAQVIG-KTEY-SKDLVGNLAGSPAIKKGISQTIKIVDELVKIMG-YLPQQIVIEMARENQ TTAQGIKNA 776 WP_004232481 704 SFKITIQEAQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-HNPQNIVIEMARENQ ITGYGRNRS 777 WP_009854540 705 PFKQIIQKSQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-DNPDNIVIEMARENQ TTNRGRSQS 778 WP_012962174 705 PFKQIIKDAQIIG-DIDD-VISVVRELPGSPAIKKGILQSVKIVDELVKVMG-HNPDNIVIEMARENQ TTNRGRNQS 778 WP_039695303 707 PFKQIIQKSQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-GNPDNIVIEMARENQ TTNRGRSQS 780 WP_014334983 704 SFKIIIQEAQVVG-DVDD-IEAVVHDLPGSPAIKKGILQSVKIVDELVKVMG-DNPDNIVIEMARENQ TTGYGRNKS 777 WP_003099269 703 DFAKIIKNEQEKTiKNES-LEETIANLAGSPAIKKGILQSIKIVDEIVKIMG-QNPDNIVIEMARENQ STMQGIKNS 777 AHY15608 703 DFAKIIKNEQEKTiKNES-LEETIANLAGSPAIKKGILQSIKIVDEIVKIMG-QNPDNIVIEMARENQ STMQGIKNS 777 AHY17476 703 DFAKIIKNEQEKTiKNES-LEETIANLAGSPAIKKGILQSIKIVDEIVKIMG-QNPDNIVIEMARENQ STMQGIKNS 777 ESR09100 -------------------------------------------------------------------- --------- AGM98575 703 DFAKIIKNEQEKTiKNES-LEETIANLAGSPAIKKGILQSIKIVDEIVKIMG-QNPDNIVIEMARENQ STMQGIKNS 777 ALF27331 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_018372492 717 DFKEIIRKAQTIE-NIDT-NQALVSSLPGSPAIKKGILQSLNIVDEIIAIMG-YAPTNIVIEMARENQ TTQKGRDNS 790 WP_045618028 703 SFKEIIQKAQVVG-KIND-VKQVVQELPGSPAIKKGILQSIKLVDELVKVMG-HAPESIVIEMARENQ TTARGKKNS 776 WP_045635197 702 SFKEIIQKAQVIG-KTDD-VKQVVQELSGSPAIKKGILQSIKIVDELVKVMG-HAPESIVIEMARENQ TTARGKKNS 775 WP_002263549 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002263887 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002264920 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002269043 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002269448 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRQNS 777 WP_002271977 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002272766 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002273241 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTKQGRRNS 777 WP_002275430 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002276448 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTKQGRRNS 777 WP_002277050 705 TFKDDIVKAQYVD-NSDD-LHQVVQSLAGSPAIKKGILQSLKIVDELVKVMG-KEPEQIVVEMARENQ TTAKGRRNS 778 WP_002277364 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002279025 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002279859 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002280230 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTKQGRRNS 777 WP_002281696 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002282247 705 TFKDDIVKAQYVD-NSDD-LHQVVQSLAGSPAIKKGILQSLKIVDELVKVMG-KEPEQIVVEMARENQ TTAKGRRNS 778 WP_002282906 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002283846 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002287255 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002288990 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002289641 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002290427 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSLAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002295753 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTKQGRRNS 777 WP_002296423 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002304487 714 SFKEEIAKAQVIG-EMDG-LNQVVSDIAGSPAIKKGILQSLKIVDELVKVMG-HNPANIVIEMARENQ TTAKGRRSS 787 WP_002305844 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTKQGRRNS 777 WP_002307203 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002310390 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSLAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_002352408 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGQRNS 777 WP_012997688 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_014677909 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019312892 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019313659 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019314093 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019315370 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019803776 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_019805234 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSLAIKKGILQNLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_024783594 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_024784288 705 TFKDDIVKAQYVD-NSDD-LHQVVQSLAGSPAIKKGILQSLKIVDELVKVMG-KEPEQIVVEMARENQ TTAKGRRNS 778 WP_024784666 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_024784894 704 SFKEEIAKAQVIG-ETDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_024786433 705 TFKDDIVKAQYVD-NSDD-LHQVVQSLAGSPAIKKGILQSLKIVDELVKVMG-KEPEQIVVEMARENQ TTAKGRRNS 778 WP_049473442 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 WP_049474547 704 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 777 EMC03581 697 SFKEEIAKAQVIG-EIDN-LNQVVSDIAGSPAIKKGILQSLKIVDELVKIMG-HQPENIVVEMARENQ FTNQGRRNS 770 WP_000428612 705 SFKEIIKKAQVVG-KTDD-VKQVVQELPGSPAIKKGILQSIKLVDELVKVMG-HEPESIVIEMARENQ TTARGKKNS 778 WP_000428613 703 SFKEITQKAQVVG-KTDD-VKQVVQELPGSPAIKKGILQSIKIVDELVKVMG-HIPESIVIEMARENQ TTARGKKNS 776 WP_049523028 702 SFKETIQKAQVVG-EIND-VKQVVQELPGSPAIKKGILQSIKIVDELVKVMG-HAPESVVIEMARENQ TTNKGKSKS 775 WP_003107102 672 DFASIIKEAQEKTiKSEK-LEETIANLAGSPAIKKGILQSVKIVDEVVKVMG-YEPSNIVIEMARENQ STQRGINNS 746 WP_054279288 705 SFKEEIKKAQEGG-LKDS-INDQIRDLAGSPAIKKGILQIINIVDEIVKIMG-KAPQHIVVEMARDVQ KTDIGVKQS 778 WP_049531101 703 SFKEIIQESQVVG-KPDD-VKQIVQELPGSSAIKKGILQSIKLVDELVKVMG-HDPESIVIEMARENQ TTARGKKNS 776 WP_049538452 703 SFKEIIQKAQVFG-KIND-VKQVVQELPGSPAIKKGILQSIKIVEELVKVMG-HEPESIVIEMARENQ TTTRGKKNS 776 WP_049549711 703 SFKKIIQKSQVVG-ETDD-VKQVVRELPGSPAIKKGILQSIKIVDELVKVMD-HAPESIVIEMARENQ TTARGKKNS 776 WP_007896501 706 DFEKLIDDAQKKAiKRES-LTEAVANLAGSPAIKKGILQSLKVVDEIVKVMG-HNPDNIVIEMSRENQ TTAQGLKNA 780 EFR44625 658 DFEKLIDDAQKKAiKRES-LTEAVANLAGSPAIKKGILQSLKVVDEIVKVMG-HNPDNIVIEMSRENQ TTAQGLKNA 732 WP_002897477 702 SFKEIIQKAQVVG-KTDD-VKQVVQELPGSPAIKKGILQSIKIVDELVKVMG-YALESIVIEMARENQ TTARGKKNS 775 WP_002906454 702 SFKEIIQKAQVVG-KTDD-VKQVVQEIPGSPAIKKGILQSIKIVDELVKVMG-HNPESIVIEMARENQ TTAKGKKNS 775 WP_009729476 703 SFKEIIQKAQVVG-KIND-VKQVVQELPGSPAIKKGILQSIKIVDELVKVMG-HAPESIVIEMARENQ TTARGKKNS 776 CQR24647 706 SFKDEIANSQVIG-DGDD-LHQVVQELAGSPAIKKGILQSLKIVDELVKVMG-YNPEQIVVEMARENQ TTARGRNNS 779 WP_000066813 705 SFKEIIQKAQVFG-KIND-VKQVVQELPGSPAIKKGILQSIKIVDELVKVMG-HAPESIVIEMARENQ TTARGKKNS 778 WP_009754323 703 SFKEIIQKAQVVG-KTDD-LIQVVRELSGSPAIKKGILQSIKIVDELVKIMG-YAPESIVIEMARENQ TTAKGKKNS 776 WP_044674937 702 SFVDEIRLAQGSG-EAED-YRAEVQNLAGSPAIKKGILQSLKIVDELIEVMG-YDPEHIVVEMARENQ FTNQGRRNS 775 WP_044676715 704 SFVDEIRLAQGSG-EAED-YRAEVQNLAGSPAIKKGILQSLKIVDELIEVMG-YDPEHIVVEMARENQ FTNQGRRNS 777 WP_044680361 704 SFVDEIRLAQGSG-EAED-YRAEVQNLAGSPAIKKGILQSLKIVDELIEVMG-YDPEHIVVEMARENQ FTNQGRRNS 777 WP_044681799 702 SFVDEIRLAQGSG-EAED-YRAEVQNLAGSPAIKKGILQSLKIVDELIEVMG-YDPEHIVVEMARENQ FTNQGRRNS 775 WP_049533112 704 SFKEEIAKAQVIG-ETDD-LNQVVSDIAGSPAIKKGILQSLKIVDELVKVMG-YNPANIVIEMARENQ TTDKGRRNS 777 WP_029090905 683 LFKKIIEEQFENEtALLN--KQRIDELAASPANKKGIWQAIKIVKELEKVLQ-QPAENIFIEFARSDE ES----KRS 752 WP_006506696 706 GYAQMIEEATSCPeDGKF-TYEEVERLAGSPALKRGIWQSLQIVEEITKVMK-CRPKYIYIEFERSEE -----KERT 776 AIT42264 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_034440723 711 SFKEKIRKAQDIN-QVND-IKEIVKDLPGSPAIKKGIYQSIRIVDEIIRKMK-DRPKNIVIEMARENQ TTQEGKNKS 784 AKQ21048 703 TFKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 WP_004636532 707 SFKKKIEDAQTIE-DITH-IYDTVAELPGSPAIKKGIRQALKIVEEIIDIIG-YEPENIVVEMARESQ TTKKGKDLS 780 WP_002364836 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_016631044 666 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 739 EMS75795 455 SFKEELANELALA-GNQS-LLEVVEALLGSPAIKKGIWQTLKIVEELIEIIG-YNPKNIVVEMARENQ RT----NRS 524 WP_002373311 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_002378009 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_002407324 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_002413717 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_010775580 717 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 790 WP_010818269 715 SFKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_010824395 715 SEKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_016622645 715 SEKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_033624816 715 SEKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_033625576 715 SEKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_033789179 715 SEKNAIQKAQSSE-HEET-LSETVNELAGSPAIKKGIYQSLKIVDELVAIMG-YAPKRIVVEMARENQ TTSTGKRRS 788 WP_002310644 715 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 788 WP_002312694 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_002314015 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_002320716 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_002330729 715 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 788 WP_002335161 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_002345439 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_034867970 711 SEKEEIAKATVES-KHKS-LVDVIQDLPGSPAIKKGIWQSLKIVEELIAIIG-YKPKNIVIEMARENQ KT----HRT 780 WP_047937432 716 SFKKEIKKAQMIT-DTEN-LEEIVKELIGSPAIKKGILQSLKIVDEIVGIMG-YEPANIVVEMARENQ TTGRGLKSS 789 WP_010720994 711 SEKEEIAKATVES-KHKS-LVDVIQDLPGSPAIKKGIWQSLKIVEELIAIIG-YKPKNIVIEMARENQ KT----HRT 780 WP_010737004 711 SEKEEIAKATVES-KHKS-LVDVIQDLPGSPAIKKGIWQSLKIVEELIAIIG-YKPKNIVIEMARENQ KT----HRT 780 WP_034700478 711 SEKEEIAKATVES-KHKS-LVDVIQDLPGSPAIKKGIWQSLKIVEELIAIIG-YKPKNIVIEMARENQ KT----HRT 780 WP_007209003 708 SFKKIIEDSQPYK-EQQS-AEEIVSELSGSPAIKKGILQSLKIVDELVAIMG-YKPKNIVVEMARENQ TTGRGKQNS 781 WP_023519017 705 SEKETIANELIMS-DSNV-LLDQVKAIPGSPAVKKGIWQSIKIVEEIIGIIG-KAPKNIVIEMARENQ RTSR----S 774 WP_010770040 708 SFKSEIAEAQSDM-NTED-LHEVVQNLAGSPAIKKGILQSLKIVDELVDIMG-SLPKNIVVEMARENQ TTSRGRTNS 781 WP_048604708 704 TEKEEIEKEQLKA-NSEEsLIEIVQNLAGSPAIKKGIFQSLKIVDELVEIMG-YAPTNIVVEMARENQ TTANGRRNS 778 WP_010750235 710 SFKEEIAKELTLS-DKQS-LLEVVEAIPGSPAIKKGIWQTLKIVEELIAIIG-YKPKNIVIEMARENQ TTTGGKNRS 783 AII16583 742 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 816 WP_029073316 718 GFKKTIDDANSTSvSGKF-SYAEVQELAGSPAIKRGIWQALLIVDEIKKIMK-HEPAHVYIEFARNED -----KERK 788 WP_031589969 718 GFKKTIDDANSTSvSGKF-SYAEVQELAGSPAIKRGIWQALLIVDEIKKIMK-HEPAHVYIEFARNED -----KERK 788 KDA45870 699 SFKETIKNAQVIE-KEET-LAKTVQELPGSPAIKKGILQSLEIVDEIIKVMG-YKPKSIVVEMARETQ --THGTRKR 771 WP_039099354 713 DFDKLITEANQMM-LAENdVQDVINDLYTSPQNKKALRQILLVVNDIQKAMKgQAPERILIEFAREDE VNPRLSVQR 788 AKP02966 707 DFKNYIENHNLNKnEDQN-ISNLVNDIHVSPALKRGITQSIKIVQEIVKFMG-HAPKYIFIEVIRETK TTSRGKRIQ 785 WP_010991369 707 SFKSIIEKEQVIT-ADKD-IQSIVADLAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_033838504 707 SFKSIIEKEQVIT-ADKD-IQSIVADLAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTGKGKNNS 780 EHN60060 710 SFKSIIEKEQVIT-ADKD-IQSIVADLAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTGKGKNNS 783 EFR89594 476 SFKSIIEKEQVIT-ADKD-IQSIVADLAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTGKGKNNS 549 WP_038409211 707 SFKSIIEKEQVST-ADKG-IQSIVAELAGSPAIKKGILQSLKIVDELVGIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 EFR95520 326 SFKSIIEKEQVST-ADKG-IQSIVAELAGSPAIKKGILQSLKIVDELVGIMG-YPPQTIVVEMARENQ TTGKGKNNS 399 WP_003723650 707 SFKSIIEKEQVST-TDKD-LQSIVAELAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_003727705 707 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_003730785 707 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_003733029 707 SFKSIIEKEQVST-TDKD-LQSIVAELAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTNKGKNNS 780 WP_003739838 707 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTVKGKNNS 780 WP_014601172 707 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_023548323 707 SFKSIIEKEQVST-ADKD-LQSIVADLAGSPAIKKGILQSLKVVEELVSVMG-YPPQTIVVEMARENQ TTNKGKNNS 780 WP_031665337 707 SFKSIIEKEQVST-TDKD-LQSIVAELAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 WP_031669209 707 SFKSIIEKEQVST-ADKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSVMG-YPPQTIVVEMARENQ TTNKGKNNS 780 WP_033920898 707 SFKSIIEKEQVST-ADKD-LQSIVADLAGSPAIKKGILQSLKVVEELVSVMG-YPPQTIVVEMARENQ TTNKGKNNS 780 AKI42028 710 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTGKGKNNS 783 AKI50529 710 SFKSIIEKEQVST-ADKD-LQSIVADLAGSPAIKKGILQSLKVVEELVSVMG-YPPQTIVVEMARENQ TTNKGKNNS 783 EFR83390 155 SFKSIIEKEQVST-TDKD-LQSIVADLAGSPAIKKGILQSLKIVDELVSIMG-YPPQTIVVEMARENQ TTVKGKNNS 228 WP_046323366 707 SFKSIIEKEQVST-ADKD-IQSIVADLAGSPAIKKGILQSLKIVDELVGIMG-YPPQTIVVEMARENQ TTGKGKNNS 780 AKE81011 719 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 793 CUO82355 710 GYAQMIEEASSCPkDGKF-TYEEVAKLAGSPALKRGIWQSLQIVEEITKVMK-CRPKYIYIEFERSEE -----KERT 780 WP_033162887 713 GYKQIIEESNMQDiEGPF-KYDEVKKLAGSPAIKRGIWQALLVVREITKFMK-HEPSHIYIEFAREEQ -----KVRK 783 AGZ01981 736 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 810 AKA60242 703 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 AKS40380 703 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 777 4UN5_B 707 TEKEDIQKAQVSG-QGDS-LHEHIANLAGSPAIKKGILQTVKVVDELVKVMGrHKPENIVIEMARENQ TTQKGQKNS 781 WP_010922251 778

841 WP_039695303 781 QQRLKKLQNSLK PSYI E----DK--VE---NSHLQNDQLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 851 WP_045635197 776 QQRYKRIEDSLK ILAS NILKENP--TD---NNQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSYDIDHI 843 5AXW_A 488 KDAQKMINEMQK QTNE EIIRTIGk--E---NAKYLIEKIKLHDMQEGKCLYSLEAIplEdlLNNPFNYEVDHI 561 WP_009880683 462 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 525 WP_010922251 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_011054416 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_011284745 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_011285506 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_011527619 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_012560673 778 RERMKRIEEGIK ELGS DILKEYP--VE---TTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_014407541 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_020905136 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_023080005 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_023610282 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_030125963 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_030126706 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_031488318 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_032460140 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_032461047 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_032462016 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_032462936 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_032464890 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_033888930 603 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 666 WP_038431314 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_038432938 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_038434062 778 RERMKRIEEGIK ELGS DILKEYP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 BAQ51233 689 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 752 KGE60162 1 ------------ ---- -------------------------------------QEL--D--INRLSGYDVDHI 16 KGE60856 ------------ ---- --------------------------------------------------------- WP_002989955 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_003030002 778 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 840 WP_003065552 781 QQRLKKLQNSLK PSYI E----DK--VE---NSHLQNDQLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 851 WP_001040076 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040078 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040080 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040081 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040083 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040085 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDDLSQYDIDHI 846 WP_001040087 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040088 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040089 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040090 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040091 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040092 779 RQRYKLLEDGVK NLAS DILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040094 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040095 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040096 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040097 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040098 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040099 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040100 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_001040104 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040105 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040106 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040107 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040108 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGETL--D--IDNLSQYDIDHI 846 WP_001040109 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_001040110 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_015058523 779 RQRYKLLEDGVK NLAS DILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_017643650 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDLI 846 WP_017647151 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGKAL--D--IDNLSQYDIDHI 846 WP_017648376 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGKAL--D--IDNLSQYDIDHI 846 WP_017649527 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_017771611 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_017771984 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 CFQ25032 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 CFV16040 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 KLJ37842 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 KLJ72361 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 KLL20707 793 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 860 KLL42645 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_047207273 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_047209694 776 RQRLTTLRESLA NLKS EKKPKYV--KDqveNHHLSDDRLFLYYLQNGKDMYTDDEL--D--IDNLSQYDIDHI 846 WP_050198062 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_050201642 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_050204027 779 RQRYKLLEEGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_050881965 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 WP_050886065 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 AHN30376 779 RQRYKLLEDGVK NLAS DILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDSLSQYDIDHI 846 EAO78426 779 RQRYKLLDDGVK NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTGEAL--D--IDNLSQYDIDHI 846 CCW42055 779 RQRYKLLDDGVR NLAS NILKEYP--TD---NQALQNERLFLYYLQNGRDMYTEKAL--D--IDNLSQYDIDHI 846 WP_003041502 778 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 840 WP_037593752 779 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 841 WP_049516684 779 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 841 GAD46167 778 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 840 WP_018363470 779 QQRLKKLQNSLK PSYI E----DK--VE---NSHLQNDQLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 849 WP_003043819 787 RERKKRIEEGIK ELES QILKENP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 850 WP_006269658 778 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 840 WP_048800889 778 QQRLKLLQDSLT PVSI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGEEL--D--IHHLSDYDIDHI 840 WP_012767106 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_014612333 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_015017095 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_015057649 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_048327215 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_049519324 777 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 840 WP_012515931 777 RQRMRKLEETAK KLGS NILKEHP--VD---NSQLQNDKRYLYYLQNGKDMYTGDDL--D--IDYLSSYDIDHI 840 WP_021320964 777 RQRMRKLEETAK KLGS NILKEHP--VD---NSQLQNDKRYLYYLQNGKDMYTGDDL--D--IDYLSSYDIDHI 840 WP_037581760 777 RQRMRKLEETAK KLGS NILKEHP--VD---NSQLQNDKRYLYYLQNGKDMYTGDDL--D--IDYLSSYDIDHI 840 WP_004232481 778 NQRLKRLQDSLK PSYV D----SK--VE---NSHLQNDRLFLYYIQNGKDMYTGEEL--D--IDHLSDYDIDHI 848 WP_009854540 779 QQRLKKLQSSLK PSYI E----DK--VE---NSHLQNDQLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 849 WP_012962174 779 QQRLKKLQDSLK PSYI E----GK--VE---NNHLQDDRLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 849 WP_039695303 781 QQRLKKLQNSLK PSYI E----DK--VE---NSHLQNDQLFLYYIQNGKDMYTGDEL--D--IDHLSDYDIDHI 851 WP_014334983 778 NQRLKRLQDSLK PSYV D----SK--VE---NSHLQNDRLFLYYIQNGKDMYTGEEL--D--IDRLSDYDIDHI 848 WP_003099269 778 RQRLRKLEEVHK NTGS KILKEYN--VS---NTQLQSDRLYLYLLQDGKDMYTGKEL--D--YDNLSQYDIDHI 841 AHY15608 778 RQRLRKLEEVHK NTGS KILKEYN--VS---NTQLQSDRLYLYLLQDGKDMYTGKEL--D--YDNLSQYDIDHI 841 AHY17476 778 RQRLRKLEEVHK NTGS KILKEYN--VS---NTQLQSDRLYLYLLQDGKDMYTGKEL--D--YDNLSQYDIDHI 841 ESR09100 ------------ ---- --------------------------------------------------------- AGM98575 778 RQRLRKLEEVHK NTGS KILKEYN--VS---NTQLQSDRLYLYLLQDGKDMYTGKEL--D--YDNLSQYDIDHI 841 ALF27331 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_018372492 791 AQRLKKIEDGIK -LGS DLLKQNP--IQd--NKDLQKEKLFLYYMQNGIDLYTGQPLncD--PDSLAFYDVDHI 857 WP_045618028 777 QQRYKRIEDALK NLAH NILKEHP--TD---NIQLQNDRLFLYYLQNGKDMYTGKSL--D--INQLSSCDIDHI 844 WP_045635197 776 QQRYKRIEDSLK ILAS NILKENP--TD---NNQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSYDIDHI 843 WP_002263549 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002263887 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002264920 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002269043 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002269448 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002271977 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002272766 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002273241 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002275430 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002276448 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002277050 779 QQRYKRLKEAIK DLNH KILKEHP--TD---NQALQNNRLFLYYLQNGRDMYTGESL--D--INRLSDYDIDHV 846 WP_002277364 778 QQRLKGLTDSIK EFGS QILKEHP--VE---HSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002279025 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002279859 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002280230 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002281696 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002282247 779 QQRYKRLKEAIK DLNH KILKEHP--TD---NQALQNNRLFLYYLQNGRDMYTGESL--D--INRLSDYDIDHV 846 WP_002282906 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002283846 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002287255 778 QQRLKGLTDSIK EFGS QILKEHP--VE---HSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002288990 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002289641 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002290427 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002295753 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002296423 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002304487 788 QKRYKRLEEAIK DLNH KILKEHP--TD---NQALQNDRLFLYYLQNGRDMYTEDPL--D--INRLSDYDIDHI 855 WP_002305844 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002307203 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002310390 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_002352408 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_012997688 778 QQRLKGLTDSIK EFGS QILKEHP--VK---HSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_014677909 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019312892 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019313659 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019314093 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019315370 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019803776 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_019805234 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_024783594 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_024784288 779 QQRYKRLKEAIK DLNH KILKEHP--TD---NQALQNNRLFLYYLQNGRDMYTGESL--D--INRLSDYDIDHV 846 WP_024784666 778 QQRLKGLTDSIK EFGS QILKEHP--VE---HSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_024784894 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_024786433 779 QQRYKRLKEAIK DLNH KILKEHP--TD---NQALQNNRLFLYYLQNGRDMYTGESL--D--INRLSDYDIDHV 846 WP_049473442 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 WP_049474547 778 QQRLKGLTDSIK EFGS QILKEHP--VE---NSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 841 EMC03581 771 QQRLKGLTDSIK EFGS QILKEHP--VE---HSQLQNDRLFLYYLQNGRDMYTGEEL--D--IDYLSQYDIDHI 834 WP_000428612 779 QQRYKRIEDSLK ILAS KILKEHP--TD---NIQLQNDRLFLYYLQNGRDMYTGKPL--D--INQLSSYDIDHI 846 WP_000428613 777 QQRYKRIEDALK NLAS NILKEHP--TN---NIQLQNDRLFLYYLQNGRDMYTGKPL--D--INQLSSYDIDHI 844 WP_049523028 776 QQRLKTLSDAIS ELG- NILKEHP--TD---NIQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSNYDIDHI 839 WP_003107102 747 RERLRKLEEVHK NIGS KILKEHE--IS---NAQLQSDRVYLYLLQDGKDMYTGKDL--D--FDRLSQYDIDHI 810 WP_054279288 779 RERMKRVQEVLK KLGS QLLKEHP--VE---NFQLQNERLYLYYLQNGKDMYTGEEL--S--ISNLSHYDIDHI 842 WP_049531101 777 QQRYKRIEDSLK ILAS NILKEHP--TD---NIQLQNDRLFLYYLQNGKDMYTGNPL--D--INHLSSYDIDHI 844 WP_049538452 777 QQRYKRIENSLK ILAS KILKEHP--TD---NNQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSCDIDHI 844 WP_049549711 777 QQRYKRIEDSLK ILAS NILKENP--TD---NNQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSYDIDHI 844 WP_007896501 781 RQRLKKIKEVHK KTGS RILEDNSerIT---NLTLQDNRLYLYLLQDGKDMYTGQDL--D--INNLSQYDIDHI 846 EFR44625 733 RQRLKKIKEVHK KTGS RILEDNSerIT---NLTLQDNRLYLYLLQDGKDMYTGQDL--D--INNLSQYDIDHI 798 WP_002897477 776 QQRYKRIEDALK NLAP NILKENP--TD---NIQLKNDRLFLYYLQNGKDMYTGKPL--D--INQLSSYDIDHI 843 WP_002906454 776 QQRYKRIEDALK NLAP NILKENP--TD---NIQLQNDRLFLYYLQNGKDMYTGKAI--D--INQLSNYDIDHI 843 WP_009729476 777 QQRYKRIEDSLK ILAS KILKEHP--TD---NIQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSCDIDHI 844 CQR24647 780 QQRLGSLTKAIQ DFGS DILKRYP--VE---NNQLQNDQLYLYYLQNGKDMYTGDTL--D--IHNLSQYDIDHI 843 WP_000066813 779 QQRYKRIEDSLK NLAS NILKENP--TD---NIQLQNDRLFLYYLQNGRDMYTGKPL--E--INQLSNYDIDHI 846 WP_009754323 777 QQRYKRIEDALK NLAP TISKENP--TD---NIQLQNDRLFLYYLQNGKDMYTGEAL--D--INQLSSYDIDHI 844 WP_044674937 776 QQRYKKIENAIK NLNS KILKEYP--TN---NQALQNDRLFLYYLQNGKDMYTDEEL--D--IDQLSQYDIDHI 843 WP_044676715 778 QQRYKKIENAIK NLNS KILKEYP--TN---NQALQNDRLFLYYLQNGKDMYTDEEL--D--IDQLSQYDIDHI 845 WP_044680361 778 QQRYKKIENAIK NLNS KILKEYP--TN---NQALQNDRLFLYYLQNGKDMYTDEEL--D--IDQLSQYDIDHI 845 WP_044681799 776 QQRYKKIENAIK NLNS KILKEYP--TN---NQALQNDRLFLYYLQNGKDMYTDEEL--D--IDQLSQYDIDHI 843 WP_049533112 778 QQRLKLLQDSLK PVNI K-----N--VE---NQQLQNDRLFLYYIQNGKDMYTGETL--D--INNLSQYDIDHI 840 WP_029090905 753 TPRDKFIEKAYA ETDT EHLKELK---Qr--SKQLSSQRLFLYFIQNGKCMYSGEHL--D--IERLDSYEVDHI 823 WP_006506696 777 ESKIKKLENVYK DEQT SVLEELKg-FDn--TKKISSDSLFLYFTQLGKCMYSGKKL--D--IDSLDKYQIDHI 849 AI T42264 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_034440723 785 KARLKKIQEGLE NLDS HVEKQAL---D---EEMLKSPKYYLYCLQNGKDIYTGKDL--D--IGQLQTYDIDHI 848 AKQ21048 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 WP_004636532 781 KERLEKLTEAIK EFDG --VKVKD--LK---NENLRNDRLYLYYLQNGRDMYTNEPL--D--INNLSKYDIDHI 845 WP_002364836 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_016631044 740 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 803 EM575795 525 KPRLKALEEALK SFDS PLLKEQP--VD---NQALQKDRLYLYYLQNGKDMYTGEAL--D--IDRLSEYDIDHI 588 WP_002373311 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_002378009 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_002407324 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_002413717 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_010775580 791 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 854 WP_010818269 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_010824395 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_016622645 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_033624816 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_033625576 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_033789179 789 IQRLKIVEKAMA EIGS NLLKEQP--TT---NEQLRDTRLFLYYMQNGKDMYTGDEL--S--LHRLSHYDIDHI 852 WP_002310644 789 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 852 WP_002312694 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_002314015 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_002320716 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_002330729 789 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 852 WP_002335161 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_002345439 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_034867970 781 SPRLKALENGLK QIGS TLLKEQP--TD---NKALQKERLYLYYLQNGRDMYTGEPL--E--IENLHQYEVDHI 844 WP_047937432 790 RPRLKALEESLK DFGS QLLKEYP--TD---NSSLQKDRLYLYYLQNGRDMYTGAPL--D--IHRLSDYDIDHI 853 WP_010720994 781 KPRLKALENGLK QIGS TLLKEQP--TD---NKALQKERLYLYYLQNGRDMYTGEPL--E--IENLHQYEVDHI 844 WP_010737004 781 SPRLKALENGLK QIGS TLLKEQP--TD---NKALQKERLYLYYLQNGRDMYTGEPL--E--IENLHQYEVDHI 844 WP_034700478 781 KPRLKALENGLK QIGS TLLKEQP--TD---NKALQKERLYLYYLQNGRDMYTGEPL--E--IENLHQYEVDHI 844 WP_007209003 782 KPRLKGIENGLK EFSD SVLKGSS--ID---NKQLQNDRLYLYYLQNGKDMYTGHEL--D--IDHLSTYDIDHI 845 WP_023519017 775 RPRLKALEEALK NIDS PLLKDYP--TD---NQALQKDRLYLYYLQNGKDMYTGEPL--E--IHRLSEYDIDHI 838 WP_010770040 782 NPRMKALEEAMR NLRS NLLKEYP--TD---NQALQNDRLYLYYLQNGKDMYTGLDL--S--LHNLSSYDIDHI 845 WP_048604708 779 RPRLKNLEKAID DLDS EILKKHP--VD---NKALQKDRLYLYYLQNGKDMYTNEEL--D--IHKLSTYDIDHI 842 WP_010750235 784 KPRLKSLEEALK NFDS QLLKERP--VD---NQSLQKDRLYLYYLQNGKDMYTGESL--D--IDRLSEYDIDHI 847 AII16583 817 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 880 WP_029073316 789 DSFVNQMLKLYK DFED EANKHLKg-EDa--KSKIRSERLKLYYTQMGKCMYTGKSL--D--IDRLDTYQVDHI 860 WP_031589969 789 DSFVNQMLKLYK DFED EANKHLKg-EDa--KSKIRSERLKLYYTQMGKCMYTGKSL--D--IDRLDTYQVDHI 860 KDA45870 772 EDRVQQIVKNLK ELPK ------P---S---NAELSDERKYLYCLQNGRDMYTGAPL--D--YDHLQFYDVDHI 833 WP_039099354 789 KRQVEQVYQNIS EL-- EIRNELK---Dl-sNSALSNTRLFLYFMQGGRDMYTGDSL--N--IDRLSTYDIDHI 856 AKP02966 786 RLQSKLLNKANG -LVP EELKKHKn--D------LSSERIMLYFLQNGKSLYSEESL--N--INKLSDYQVDHI 858 WP_010991369 781 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELRNNRLYLYYLQNGKDMYTGQDL--D--IHNLSNYDIDHI 844 WP_033838504 781 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELRNNRLYLYYLQNGKDMYTGQDL--D--IHNLSNYDIDHI 844 EHN60060 784 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELRNNRLYLYYLQNGKDMYTGQDL--D--IHNLSNYDIDHI 847 EFR89594 550 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQDL--D--IHNLSNYDIDHI 613 WP_038409211 781 KPRFISLEKAIK EFGS QILKEHP--TD---NQCLKNDRLYLYYLQNGKDMYTGKEL--D--IHNLSNYDIDHI 844 EFR95520 400 KPRFISLEKAIK EFGS QILKEHP--TD---NQCLKNDRLYLYYLQNGKDMYTGKEL--D--IHNLSNYDIDHI 463 WP_003723650 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_003727705 781 KPRYKSLEKAIK DFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDIYTGQEL--D--IHNLSNYDIDHI 844 WP_003730785 781 KPRYKSLEKAIK DFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDIYTGQEL--D--IHNLSNYDIDHI 844 WP_003733029 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_003739838 781 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELRNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_014601172 781 KPRYKSLEKAIK EFGS KILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_023548323 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_031665337 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_031669209 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 WP_033920898 781 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 844 AKI42028 784 KPRYKSLEKAIK EFGS KILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 847 AKI50529 784 KPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHI 847 EFR83390 229 RPRYKSLEKAIK EFGS QILKEHP--TD---NQELKNNRLYLYYLQNGKDIYTGQEL--D--IHNLSNYDIDHI 292 WP_046323366 781 KPRFTSLEKAIK ELGS QILKEHP--TD---NQGLKNDRLYLYYLQNGKDMYTGQEL--D--IHNLSNYDIDHV 844 AKE81011 794 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 857 CUO82355 781 ESKIKKLENVYK DEQT SVLEELKg-FDn--TKKISSDSLFLYFTQLGKCMYSGKKL--D--IDSLDKYQIDHI 853 WP_033162887 784 ESKIAKLQKIYE NLQT QVYESLKk-EDa--KKRMETDALYLYYLQMGKSMYSGKPL--D--IDKLSTYQIDHI 855 AGZ01981 811 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 874 AKA60242 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDAI 841 AKS40380 778 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDHI 841 4UN5_B 782 RERMKRIEEGIK ELGS QILKEHP--VE---NTQLQNEKLYLYYLQNGRDMYVDQEL--D--INRLSDYDVDAI 845 WP_010922251 842

910 WP_039695303 852 IPQAFIKDDSIDNRVLISSAKNRG-KSDD--VP S--LDIVRARKA-EWVRLYKSGLISKRKEDNLTKA--ERGGLTE 920 WP_045635197 844 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--IEVVQKRKA-FWQQLLDSKLISERKFNNLTKA--ERGGLDE 912 5AXW_A 562 IPRSVSFDNSFNNKVLVKQEEASK-KGNR--TP Fqy-LSSSDSKI-SYETFKKHILNLAKGKGRISKTk-KEYLLEE 632 WP_009880683 526 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 594 WP_010922251 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_011054416 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_011284745 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSNN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_011285506 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_011527619 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_012560673 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_014407541 841 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 909 WP_020905136 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_023080005 841 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 909 WP_023610282 841 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 909 WP_030125963 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_030126706 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_031488318 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_032460140 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_032461047 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_032462016 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_032462936 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_032464890 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_033888930 667 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 735 WP_038431314 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_038432938 841 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 909 WP_038434062 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWKQLLNAKLITQRKFDNLTKA--ERGGLSE 910 BAQ51233 753 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 821 KGE60162 17 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 85 KGE60856 --------------------------------- -------------------------------------------- WP_002989955 842 VPQSFIKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_003030002 841 IPQAFIKDNSLDNRVLIRSDKNRG-KSDD--VP S--IEVVHEMKS-FWSKLLSVKLITQRKFDNLTKA--ERGGLTE 909 WP_003065552 852 IPQAFIKDDSIDNRVLISSAKNRG-KSDD--VP S--LDIVRARKA-EWVRLYKSGLISKRKEDNLTKA--ERGGLTE 920 WP_001040076 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040078 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040080 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040081 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040083 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040085 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040087 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040088 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040089 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040090 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040091 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040092 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--IDIVKARKA-FWKKLLDAKLISQRKYDNLTKA--ERGGLTP 915 WP_001040094 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040095 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040096 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040097 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040098 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040099 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040100 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040104 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040105 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040106 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040107 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040108 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040109 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_001040110 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_015058523 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--IDIVKARKA-FWKKLLDAKLISQRKYDNLTKA--ERGGLTP 915 WP_017643650 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_017647151 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_017648376 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_017649527 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_017771611 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_017771984 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 CFQ25032 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 CFV16040 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 KLJ37842 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 KLJ72361 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 KLL20707 861 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 929 KLL42645 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_047207273 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_047209694 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--VEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_050198062 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_050201642 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_050204027 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDN--VP S--IDIVKARKA-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_050881965 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_050886065 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 AHN30376 847 VPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--IDIVKARKA-FWKKLLDAKLISQRKYDNLTKA--ERGGLTP 915 EAO78426 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 CCW42055 847 IPQAFIKDDSIDNRVLVSSAKNRG-KSDD--VP S--LEIVKDCKV-FWKKLLDAKLMSQRKYDNLTKA--ERGGLTS 915 WP_003041502 841 IPQAYIKDDSFDNRVLTSSSENRG-KSDN--VP S--IEVVCARKA-DWMRLRKAGLISQRKEDNLTKA--ERGGLTE 909 WP_037593752 842 IPQAFIKDNSLDNRVLTRSDKNRG-KSDD--VP S--IEVVHEMKS-FWSKLLSVKLITQRKEDNLTKA--ERGGLTE 910 WP_049516684 842 IPQAFIKDNSLDNRVLTRSDKNRG-KSDD--VP S--IEVVHEMKS-FWSKLLSVKLITQRKEDNLTKA--ERGGLTE 910 GAD46167 841 IPQAFIKDNSLDNRVLTRSDKNRG-KSDD--VP S--IEVVHEMKS-FWSKLLSVKLITQRKEDNLTKA--ERGGLTE 909 WP_018363470 850 IPQAFIKDDSIDNRVLTSSAKNRG-KSDD--VP S--LGIVRARKA-EWVRLYKSGLISKRKEDNLTKA--ERGGLTE 918 WP_003043819 851 VPQSFIKDDSIDNKVLTRSVENRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 919 WP_006269658 841 IPQAFIKDNSLDNRVLTRSDKNRG-KSDD--VP S--IEVVHEMKS-FWSKLLSVKLITQRKEDNLTKA--ERGGLTE 909 WP_048800889 841 IPQAFIKDDSIDNRVLTSSAKNRG-KSDN--VP N--LEVVCDRKA-DWIRLREAGLISQRKEDNLTKA--ERGGLTE 909 WP_012767106 841 VPQSFIKDDSIDNKILTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_014612333 841 VPQSFIKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_015017095 841 VPQSFIKDDSIDNKVLTRSDKNRG-KSDD--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_015057649 841 VPQSFIKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_048327215 841 VPQSFIKDDSIDNKVLTRSDKNRG-KSDD--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_049519324 841 VPQSFIKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 909 WP_012515931 841 IPQSFIKNNSIDNKVLTSQGANRG-KLDN--VP S--EAIVRKMKG-YWQSLLRAGAISKQKEDNLTKA--ERGGLIQ 909 WP_021320964 841 IPQSFIKNNSIDNKVLTSQGANRG-KLDN--VP S--EAIVRKMKG-YWQSLLRAGAISKQKEDNLTKA--ERGGLIQ 909 WP_037581760 841 IPQSFIKNNSIDNKVLTSQGANRG-KLDN--VP S--EAIVRKMKG-YWQSLLRAGAISKQKEDNLTKA--ERGGLIQ 909 WP_004232481 849 IPQAFIKDNSIDNRVLTSSAKNRG-KSDD--VP S--IEIVRNRKS-YWYKLYKSGLISKRKEDNLTKA--ERGGLTE 917 WP_009854540 850 IPQAFIKDDSIDNRVLTSSAKNRG-KSDD--VP S--LDIVRARKA-EWVRLYKSGLISKRKEDNLTKA--ERGGLTE 918 WP_012962174 850 IPQAFIKDDSIDNRVLTSSAKNRG-KSDD--VP S--LDIVHDRKA-DWIRLYKSGLISKRKEDNLTKA--ERGGLTE 918 WP_039695303 852 IPQAFIKDDSIDNRVLTSSAKNRG-KSDD--VP S--LDIVRARKA-EWVRLYKSGLISKRKEDNLTKA--ERGGLTE 920 WP_014334983 849 IPQAFIKDNSIDNKVLTSSAKNRG-KSDD--VP S--IEIVRNRRS-YWYKLYKSGLISKRKEDNLTKA--ERGGLTE 917 WP_003099269 842 IPQSFIKDNSIDNIVLITQASNRG-KSDN--VP N--IETVNKMKS-FWYKQLKSGAISQRKFDHLTKA--ERGALSD 910 AHY15608 842 IPQSFIKDNSIDNIVLITQASNRG-KSDN--VP N--IETVNKMKS-FWYKQLKSGAISQRKFDHLTKA--ERGALSD 910 AHY17476 842 IPQSFIKDNSIDNIVLITQASNRG-KSDN--VP N--IETVNKMKS-FWYKQLKSGAISQRKFDHLTKA--ERGALSD 910 ESR09100 --------------------------------- -------------------------------------------- AGM98575 842 IPQSFIKDNSIDNIVLITQASNRG-KSDN--VP N--IETVNKMKS-FWYKQLKSGAISQRKFDHLTKA--ERGALSD 910 ALF27331 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_018372492 858 VPRSYIKNDSFDNKVLITSKGNRK-KLDD--VP A--KEVVEKMEN-TWRRLHAAGLISDIKLSYLMKGe-----LTE 923 WP_045618028 845 IPQAFIKDDSLDNRVLTSSKDNRG-KSDN--VP S--LEIVQKRKA-FWQQLLDSKLISERKENNLTKA--ERGGLDE 913 WP_045635197 844 IPQAFIKDDSLDNRVLTSSKDNRG-KSDN--VP S--IEVVQKRKA-FWQQLLDSKLISERKENNLTKA--ERGGLDE 912 WP_002263549 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002263887 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002264920 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKG--ERGGLTD 910 WP_002269043 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002269448 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--EDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002271977 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002272766 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKP-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002273241 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002275430 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKP-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002276448 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002277050 847 IPQAFIKDNSIDNRVLTSSKANRG-KSDD--VP S--EDVVNRMRP-FWNKLLSSGLISQRKYNNLTKK--E---LTP 912 WP_002277364 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002279025 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKP-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002279859 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKG--ERGGLTD 910 WP_002280230 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002281696 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002282247 847 IPQAFIKDNSIDNRVLTSSKANRG-KSDD--VP S--EDVVNRMRP-FWNKLLSSGLISQRKYNNLTKK--E---LTL 912 WP_002282906 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002283846 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002287255 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002288990 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002289641 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002290427 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKG--ERGGLTD 910 WP_002295753 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002296423 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002304487 856 IPQAFIKDNSIDNRVLTRSDKNRG-KSDD--VP S--EEVVHKMKP-FWSKLLSAKLITQRKEDNLTKA--ERGGLTD 924 WP_002305844 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002307203 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_002310390 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKG--ERGGLTD 910 WP_002352408 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_012997688 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_014677909 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019312892 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019313659 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019314093 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019315370 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKP-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019803776 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_019805234 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKG--ERGGLTD 910 WP_024783594 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_024784288 847 IPQAFIKDNSIDNRVLTSSKANRG-KSDD--VP S--EDVVNRMRP-FWNKLLSSGLISQRKYNNLTKK--E---LTL 912 WP_024784666 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_024784894 842 IPQAFIKDNSIDNRVLTSSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKEDNLTKA--ERGGLTD 910 WP_024786433 847 IPQAFIKDNSIDNRVLTSSKANRG-KSDD--VP S--EDVVNRMRP-FWNKLLSSGLISQRKYNNLTKK--E---LTL 912 WP_049473442 842 IPQAFIKDNSIDNRVLISSKENRG-KSDD--VP S--KDVVRKMKP-YWSKLLSAKLITQRKFDNLTKA--ERGGLTD 910 WP_049474547 842 IPQAFIKDNSIDNRVLISSKENRG-KSDD--VP S--KDVVRKMKS-YWSKLLSAKLITQRKFDNLTKA--ERGGLTD 910 EMC03581 835 IPQAFIKDNSIDNRVLISSKENRG-KSDD--VP S--KNVVRKMKS-YWSKLLSAKLITQRKFDNLTKA--ERGGLTD 903 WP_000428612 847 VPQAFIKDDSLDNRVLISLKDNRG-KSDN--VP S--LEVVEKMKT-FWQQLLDSKLISYRKFNNLTKA--ERGGLDE 915 WP_000428613 845 VPQAFIKDDSLDNRVLISLKDNRG-KSDN--VP S--IEVVQKRKA-FWQQLLDSKLISERKFNNLTKA--ERGGLDE 913 WP_049523028 840 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEIVEKMKG-FWQQLLDSKLISERKFNNLTKA--ERGGLDE 908 WP_003107102 811 IPQSFIKDNSIDNIVLISQESNRG-KSDN--VP Y--IAIVNKMKS-YWQHQLKSGAISQRKFDNLTKA--ERGGLSE 879 WP_054279288 843 IPRSFIKDDSIDNKVLIRSEHNRG-KIDN--VP S--IEVVKRMKP-YWQKLLDTKVISQRKEDNLTKA--ERGGLQE 911 WP_049531101 845 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEVVQKRKA-FWQQLLESKLISERKFNNLTKA--ERGGLNE 913 WP_049538452 845 IPQAFIKDDSLDNRVLISSKENRG-KSDN--VP C--LEVVDKMKV-FWQQLLDFKLISYRKFNNLTKA--ERGGLDE 913 WP_049549711 845 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEVVQKRKA-FWQQLLDSKLISERKFNNLTKAerERDGLNE 915 WP_007896501 847 IPQSFIKDNSIDNLVLITQKANRG-KSDN--VP S--IEVVRDMKDrVWRRQLANGAISRQKFDHLTKA--ERGGLAD 916 EFR44625 799 IPQSFIKDNSIDNLVLITQKANRG-KSDN--VP S--IEVVRDMKDrVWRRQLANGAISRQKFDHLTKA--ERGGLAD 868 WP_002897477 844 IPQAFIKDDSIDNRVLISSKDNRG-KSDN--VP S--LEVVQKRKA-FWQQLLDSKLISERKFNNLTKA--ERGGLDE 912 WP_002906454 844 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--IEVVQKRKA-FWQQLLDSKLISERKFNNLTKA--KRGGLDE 912 WP_009729476 845 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEVVDKMKV-FWQQLLDSKLISYRKFNNLTKA--ERGGLNE 913 CQR24647 844 IPQSFIKDNSLDNRVLINSKSNRG-KSDN--VP S--NEVVKRMKG-FWLKQLDAKLISQRKEDNLTKA--ERGGLSA 912 WP_000066813 847 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEVVEKMKA-FWQQLLDSKLISERKFNNLTKAerERGGLNE 917 WP_009754323 845 IPQAFIKDDSLDNRVLISSKDNRG-KSDN--VP S--LEVVKKRKA-FWQQLLDSKLISERKFNNLTKA--ERGGLDE 913 WP_044674937 844 IPQAFIKDDSLDNKVLIKSAKNRG-KSDD--VP S--LEIVHKKKN-FWKQLLDSQLISQRKFDNLTKA--ERGGLTN 912 WP_044676715 846 IPQAFIKDDSLDNKVLIKSAKNRG-KSDD--VP S--LEIVHKKKN-FWKQLLDSQLISQRKFDNLTKA--ERGGLTN 914 WP_044680361 846 IPQAFIKDDSLDNKVLIKSAKNRG-KSDD--VP S--LEIVHKKKN-FWKQLLDSQLISQRKFDNLTKA--ERGGLTN 914 WP_044681799 844 IPQAFIKDDSLDNKVLIKSAKNRG-KSDD--VP S--LEIVHKKKN-FWKQLLDSQLISQRKFDNLTKA--ERGGLTN 912 WP_049533112 841 IPQAFIKDDSFDNRVLISSSENRG-KSDN--VP S--IEVVRARKA-DWMRLRKAGLISQRKEDNLTKA--ERGGLTE 909 WP_029090905 824 LPQSYIKDNSIENLALVKKVENQR-KKDSllLN S---SIINQNYS-RWEQLKNAGLIGEKKFRNLTRTk-----ITD 890 WP_006506696 850 VPQSLVKDDSEDNRVLVVPSENQR-KLDDlvVP ---FDIRDKMYR-FWKLLFDHELISPKKFYSLTKTe-----YTE 916 AIT42264 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_034440723 849 IPRSFITDNSFDNLVLISSIVNRG-KLDN--VP Sp--DIVRQQKG-FWKQLLRAGLMSQRKENNLTKGk-----LTD 914 AKQ21048 842 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 WP_004636532 846 IPQSFTIDNSIDNKVLVSRTKNQGnKSDD--VP S--INIVHKMKP-FWRQLHKAGLISDRKFKNLTKA--EHGGLTE 915 WP_002364836 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_016631044 804 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 872 EMS75795 589 IPRSFIVDNSIDNKVLVSSKENRL-KMDD--VP D--QKVVIRMRR-YWEKLLRANLISERKFAYLTKLe-----LTP 654 WP_002373311 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KKVVKKMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_002378009 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_002407324 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_002413717 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_010775580 855 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKKMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 923 WP_010818269 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_010824395 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_016622645 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKDMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_033624816 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKKMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_033625576 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKKMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_033789179 853 IPQSFMKDDSLDNLVLVGSTENRG-KSDD--VP S--KEVVKKMKA-YWEKLYAAGLISQRKFQRLTKG--EQGGLTL 921 WP_002310644 853 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 918 WP_002312694 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_002314015 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_002320716 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_002330729 853 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 918 WP_002335161 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_002345439 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_034867970 845 IPRSFIVDNSIDDKVLVASKQNQK-KRDD--VP K--KQIVNEQRI-FWNQLKEAKLISTKKYAYLTKIe-----LTP 910 WP_047937432 854 IPRSFTIDNSIDNKVLVSSKENRL-KKDD--VP S--EKVVKKMRS-FWYDLYSSKLISKRKLDNLTKIk-----LTE 919 WP_010720994 845 IPRSFIVDNSIDNKVLVASKQNQK-KRDD--VP K--KQIVNEQRI-FWNQLKEAKLISPKKYAYLTKIe-----LTP 910 WP_010737004 845 IPRSFIVDNSIDNKVLVASKQNQK-KRDD--VP K--KQIVNEQRI-FWNQLKEAKLISPKKYAYLTKIe-----LTP 910 WP_034700478 845 IPRSFIVDNSIDNKVLVASKQNQK-KRDD--VP N--KQIVNEQRI-FWNQLKEAKLISPKKYAYLTKIe-----LTP 910 WP_007209003 846 IPQSFLIDNSIDNRVLITSKSNRG-KSDN--VP S--EEVVRKMDR-FWRKLLNAKLISERKYTNLTKKe-----LTE 911 WP_023519017 839 IPRSFIVDNSLDNKVLVSSKVNRG-KLDN--AP D--PLVVKRMRS-HWEKLHQAKLISDKKLANLTKQn-----LTE 904 WP_010770040 846 VPQSFTTDNSLDNRVLVSSKENRG-KKDD--VP S--KEVVQKNIT-LWETLKNSNLISQKKYDNLTKG--LRGGLTE 914 WP_048604708 843 IPQSFIVDNSLDNRVLVSSSKNRG-KLDD--VP S--KEVVKKMRA-FWESLYRSGLISKKKEDNLVKA--ESGGLSE 911 WP_010750235 848 IPRSFIVDHSLDNKVLVSSKENRL-KKDD--VP D--SKVVKRMKA-YWEKLLRANLISERKESYLIKLe-----LTD 913 AII16583 881 VPQSFLKDDSIDNKVLIRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKEDNLTKA--ERGGLSE 949 WP_029073316 861 VPQSLLKDDSIDNKVLVLSSENQR-KLDDlvIP ---EMIRNKMFG-FWNKLYENKIISPKKFYSLIKSe-----YSD 927 WP_031589969 861 VPQSLLKDDSIDNKVLVLSSENQR-KLDDlvIP ---SSIRNKMYG-FWEKLENNKIISPKKFYSLIKTe-----FNE 927 KDA45870 834 IPQSFLKDDSIENKVLTIKKENVR-KTNG--LP S--EAVIQKMGS-FWKKLLDAGAMTNKKYDNLRRNl--HGGLNE 902 WP_039099354 857 LPQSFIKDNSLDNRVLVSQRMNRS-KADQ--VP S--VELGQKMQI-QWEQMLRAGLITKKKYDNLTLNp-------- 923 AKP02966 859 LPRTYIPDDSLENKALVLAKENQR-KADDllLN S---NVIDKNLE-RWTYMLNNNMMGLKKEKNLTRRv-----ITD 925 WP_010991369 845 VPQSFITDNSIDNLVLISSAGNRE-KGDD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_033838504 845 VPQSFITDNSIDNLVLISSAGNRE-KGDD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 EHN60060 848 VPQSFITDNSIDNLVLISSAGNRE-KGDD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 916 EFR89594 614 VPQSFITDNSIDNLVLISSAGNRE-KGND--VP P--LEIVQKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 682 WP_038409211 845 IPQSFITDNSIDNRVLVSSTANRE-KGDN--VP L--LEVVRKRKA-FWEKLYQAKLMSKRKFDYLTKA--ERGGLTE 913 EFR95520 464 IPQSFITDNSIDNRVLVSSTANRE-KGDN--VP L--LEVVRKRKA-FWEKLYQAKLMSKRKFDYLTKA--ERGGLTE 532 WP_003723650 845 VPQSFITDNSIDNLVLISSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_003727705 845 VPQSFITDNSIDNLVLISSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_003730785 845 VPQSFITDNSIDNLVLISSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_003733029 845 VPQSFITDNSVDNLVLISSAGNRE-KGDN--VP P--LEIVQKRKI-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_003739838 845 VPQSFITDNSIDNLVLISSAGNRE-KGDD--VP P--LEIVRKRKV-FWEKLFQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_014601172 845 VPQSFITDNSIDNLVLTSSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTD 913 WP_023548323 845 VPQSFITDNSIDNLVLTSSAGNRE-KGDN--VP P--LEIVQKRKI-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_031665337 845 VPQSFITDNSIDNLVLTSSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_031669209 845 VPQSFITDNSVDNLVLTSSAGNRE-KGDN--VP P--LEIVQKRKI-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 WP_033920898 845 VPQSFITDNSIDNLVLTSSAGNRE-KGDN--VP P--LEIVQKRKI-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 913 AKI42028 848 VPQSFITDNSIDNLVLTSSAGNRE-KGGD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTD 916 AKI50529 848 VPQSFITDNSIDNLVLTSSAGNRE-KGDN--VP P--LEIVQKRKI-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 916 EFR83390 293 VPQSFITDNSIDNLVLTSSAGNRE-KGDD--VP P--LEIVRKRKV-FWEKLYQGNLMSKRKFDYLTKA--ERGGLTE 361 WP_046323366 845 VPQSFITDNSIDNRVLASSAANRE-KGDN--VP S--LEVVRKRKV-YWEKLYQAKLMSKRKFDYLTKA--ERGGLTE 913 AKE81011 858 VPQSFLKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 926 CUO82355 854 VPQSLVKDDSEDNRVLVLPSENQR-KLDDlvVP ---FDIRDKMYR-FWKLLFDHELISPKKFYSLIKTe-----YTE 920 WP_033162887 856 LPQSLIKDDSFDNRVLVLPEENQW-KLDSetVP ---FEIRNKMIG-FWQMLHENGLMSNKKFFSLIRTd-----FSD 922 AGZ01981 875 VPQSFLKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 943 AKA60242 842 VPQSFLKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 AKS40380 842 VPQSFLKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 910 4UN5_B 846 VPQSFLKDDSIDNKVLTRSDKNRG-KSDN--VP S--EEVVKKMKN-YWRQLLNAKLITQRKFDNLTKA--ERGGLSE 914 WP_010922251 911

981 WP_039695303 921 AD KAGFIKRQLVETRQITKHVAQILDARFNTEHDENDKVIR--DVKVITLKSNLVSQFRKDF EFYKVREINDY 991 WP_045635197 913 RD KVGFIKRQLVETRQITKHVAQILDARYNTEVNEKDKKNR--TVKIITLKSNLVSNFRKEF RLYKVREINDY 983 5AXW_A 633 RD QKDFINRNLVDTRYATRGLMNLLRSYFR---------VNnlDVKVKSINGGFTSFLRRKW KFKKERNKGYK 702 WP_009880683 595 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 665 WP_010922251 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_011054416 911 LD KVGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_011284745 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_011285506 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_011527619 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_012560673 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_014407541 910 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_020905136 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_023080005 910 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_023610282 910 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_030125963 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_030126706 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_031488318 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_032460140 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_032461047 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_032462016 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_032462936 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_032464890 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_033888930 736 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 806 WP_038431314 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_038432938 910 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_038434062 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 BAQ51233 822 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 892 KGE60162 86 LD KVGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVRVITLKSKLVSDFRKDF QFYKVREINNY 156 KGE60856 -- ------------------------------------------------------------ ----------- WP_002989955 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 WP_003030002 910 ED KAGFIKRQLVETRQITKHVAQILDERFNIEFDGNKRRIR--NVKIITLKSNLVSNFRKEF ELYKVREINDY 980 WP_003065552 921 AD KAGFIKRQLVETRQITKHVAQILDARFNTESDENDKVIR--DVKVITLKSNLVSQFRKDF EFYKVREINDY 991 WP_001040076 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF VFYKIREVNNY 986 WP_001040078 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_001040080 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040081 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040083 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040085 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040087 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040088 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040089 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040090 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040091 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040092 916 DD KAGFIQRQLVETRQITKHVARILDERFNNKVDDNNKPIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040094 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040095 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040096 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040097 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040098 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040099 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040100 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040104 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040105 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_001040106 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_001040107 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_001040108 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_001040109 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_001040110 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_015058523 916 DD KAGFIQRQLVETRQITKHVARILDERFNNKVDDNNKPIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_017643650 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_017647151 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_017648376 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_017649527 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_017771611 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_017771984 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 CFQ25032 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 CFV16040 916 DD KARFIQRQLVEIRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 KLJ37842 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTVKSNLVSNFRKEF GFYKIREVNNY 986 KLJ72361 916 DD KARFIQRQLVETRQITKHVARILDELFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 KLL20707 930 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 1000 KLL42645 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_047207273 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_047209694 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_050198062 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_050201642 916 DD KARFIQRQLVETRQITKHVASILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_050204027 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_050881965 916 DD KARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 WP_050886065 916 DD KARFIQRQLVETRQIIKHVARILDERENNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 AHN30376 916 DD KAGFIQRQLVETRQITKHVARILDERFNNKVDDNNKPIR--KVKIVILKSNLVSNERKEF GFYKIREVNNY 986 EAO78426 916 DD KARFIQRQLVETRQIIKHVARILDERENNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNNY 986 CCW42055 916 DD KARFIQRQLVETRQIIKHVARILDERENNELDSKGRRIR--KVKIVTLKSNLVSNFRKEF GFYKIREVNDY 986 WP_003041502 910 ND KAGFIKRQLVETRQITKHVAQVLDARFNAKHDENKKVIR--DVKIITLKSNLVSQFRKDF KFYKVREINDY 980 WP_037593752 911 ED KAGFIKRQLVETRQIIKHVAQILDERENTEEDGAQRRIR--NVKIITLKSNLVSNERKEF ELYKVREINDY 981 WP_049516684 911 ED KAGFIKRQLVETRQIIKHVAQILDERENTEEDGAQRRIR--NVKIITLKSNLVSNERKEF ELYKVREINDY 981 GAD46167 910 ED KAGFIKRQLVETRQIIKHVAQILDERENTEEDGAQRRIR--NVKIITLKSNLVSNERKEF ELYKVREINDY 980 WP_018363470 919 AD KAGFIKRQLVETRQIIKHVAQILDARENTERDENDKVIR--DVKVITLKSNLVSQFRKEF KFYKVREINDY 989 WP_003043819 920 AD KAGFIKRQLVETRQITKHVARILDSRMNTKRDKNDKPIR--EVKVITLKSKLVSDFRKDF QLYKVRDINNY 990 WP_006269658 910 ED KAGFIKRQLVETRQIIKHVAQILDERENTEEDGNKRRIR--NVKIITLKSNLVSNERKEF ELYKVREINDY 980 WP_048800889 910 ND KAGFIHRQLVETRQITKHVAQILDARFNPKRDDNKKVIR--DVKIITLKSNLVSQFRRDF KLYKVREINDY 980 WP_012767106 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_014612333 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_015017095 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_015057649 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_048327215 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_049519324 910 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 980 WP_012515931 910 VD KAGFIQRQLVETRQIIKHVAQILDSRENTEFDDHNKRIR--KVHIITLKSKLVSDERKEF GLYKIRDINHY 980 WP_021320964 910 VD KAGFIQRQLVETRQIIKHVAQILDSRENTEFDDHNKRIR--KVHIITLKSKLVSDERKEF GLYKIRDINHY 980 WP_037581760 910 VD KAGFIQLQLVETRQIIKHVAQILDSRENTEFDDHNKRIR--KVHIITLKSKLVSDERKEF GLYKIRDINHY 980 WP_004232481 918 TD KAGFIKRQLVETRQIIKHVAQILDARENTKCDENDKVIR--DVKVITLKSSLVSQFRKEF KFYKVREINDY 988 WP_009854540 919 AD KAGFIKRQLVETRQIIKHVAQILDARENTEHDENDKVIR--DVKVITLKSNLVSQFRKDF EFYKVREINDY 989 WP_012962174 919 ND KAGFIKRQLVETRQIIKHVAQILDSRENTERDENDKVIR--NVKVITLKSNLVSQFRKDF KFYKVREINDY 989 WP_039695303 921 AD KAGFIKRQLVETRQIIKHVAQILDARENTEHDENDKVIR--DVKVITLKSNLVSQFRKDF EFYKVREINDY 991 WP_014334983 918 AD KAGFIKRQLVETRQIIKHVAQILDARENTKRDENDKVIR--DVKVITLKSNLVSQFRKEF KFYKVREINDY 988 WP_003099269 911 FD KAGFIKRQLVETRQIIKHVAQILDSRENSNLTEDSKSNR--NVKIITLKSKMVSDERKDF GFYKLREVNDY 981 AHY15608 911 FD KAGFIKRQLVETRQIIKHVAQILDSRENSNLTEDSKSNR--NVKIITLKSKMVSDERKDF GFYKLREVNDY 981 AHY17476 911 FD KAGFIKRQLVETRQIIKHVAQILDSRENSNLTEDSKSNR--NVKIITLKSKMVSDERKDF GFYKLREVNDY 981 ESR09100 -- ------------------------------------------------------------ ----------- AGM98575 911 FD KAGFIKRQLVETRQIIKHVAQILDSRENSNLTEDSKSNR--NVKIITLKSKMVSDERKDF GFYKLREVNDY 981 ALF27331 911 DD KAGFIKRQLVETRQIIKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_018372492 924 ED KAGFIRRQLVETRQITKHVARLLDEKLNRKKNENGEKLR--TTKIITLKSVFASRFRANF DLYKLRELNHY 994 WP_045618028 914 RD KVGFIKRQLVETRQIIKHVAQILDARENTEVTEKDKKDR--SVKIITLKSNLVSNERKEF RLYKVREINDY 984 WP_045635197 913 RD KVGFIKRQLVETRQIIKHVAQILDARYNTEVNEKDKKNR--TVKIITLKSNLVSNERKEF RLYKVREINDY 983 WP_002263549 911 DD KAGFIKRQLVETRQIIKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002263887 911 DD KAGFIKRQLVETRQIIKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002264920 911 DD KAGFIKRQLVETRQIIKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002269043 911 DD KAGFIKRQLVETRQIIKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002269448 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002271977 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002272766 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002273241 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002275430 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002276448 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002277050 913 DD KAGFIKRQLVETRQITKHVARMLDERFNKEFDDNNKRIR--RVKIVTLKSNLVSSFRKEF ELYKVREINDY 983 WP_002277364 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_002279025 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002279859 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002280230 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002281696 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_002282247 913 DD KAGFIKRQLVETRQITKHVARMLDERFNKEFDDNNKRIR--RVKIVTLKSNLVSSFRKEF ELYKVREINDY 983 WP_002282906 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002283846 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_002287255 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_002288990 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002289641 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002290427 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002295753 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002296423 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_002304487 925 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 995 WP_002305844 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002307203 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002310390 911 DD KAGFIKHQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_002352408 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_012997688 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_014677909 911 DD KAGFIKRQLVETRQITKHVARILDERFYTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_019312892 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_019313659 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_019314093 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_019315370 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_019803776 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_019805234 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_024783594 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_024784288 913 DD KAGFIKRQLVETRQITKHVARMLDERFNKEFDDNNKRIR--RVKIVTLKSNLVSSFRKEF ELYKVREINDY 983 WP_024784666 911 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVTLKSNLVSNFRKEF ELYKVREINDY 981 WP_024784894 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVTLKSNLVSNERKEF ELYKVREINDY 981 WP_024786433 913 DD KAGFIKRQLVETRQITKHVARMLDERFNKEFDDNNKRIR--RVKIVTLKSNLVSSFRKEF ELYKVREINDY 983 WP_049473442 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVILKSNLVSNERKEF ELYKVREINDY 981 WP_049474547 911 DD KAGFIKRQLVETRQITKHVARILDERENTETDENNKKIR--QVKIVILKSNLVSNERKEF ELYKVREINDY 981 EMC03581 904 DD KAGFIKRQLVETRQITKHVARILDERFHTETDENNKKIR--QVKIVILKSNLVSNERKEF ELYKVREINDY 974 WP_000428612 916 RD KVGFIKRQLVETRQITKHVAQILDARYNTEVNEKDKKNR--TVKIITLKSNLVSNFRKEF RLYKIREINDY 986 WP_000428613 914 RD KVGFIKRQLVETRQITKHVAQILDARFNKEVNEKDKKNR--TVKIITLKSNLVSNFRKEF RLYKVREINDY 984 WP_049523028 909 RD KVGFIKRQLVETRQITKHVAQILDDRFNAEVNEKNQKLR--SVKIITLKSNLVSNFRKEF GLYKVREINDY 979 WP_003107102 880 YD KAGFIKRQLVETRQITKHVAQILNNRENNNVDDSSKNKR--PVKIITLKSKMVSDERKEF GFYKIREVNDY 950 WP_054279288 912 SD KANFIQRQLVETRQITKHVAQILDSRENTERDEKDRPIR--RVKVITLKSKEVSDFRQDF GFYKLREINDY 982 WP_049531101 914 RD KVGFIKRQLVETRQITKHVAQILDSRFNIKVNEKNQKIR--TVKIITLKSNLVSNFRKEF RLYKVREINDY 984 WP_049538452 914 RD KVGFIRRQLVETRQITKHVAQILDSRFNIEVTEKDKKNR--NVKIITLKSNLVSNFRKEF GLYKVREINDY 984 WP_049549711 916 LD KVGFIKRQLVETRQITKHVAQILDARFNKEVTEKDKKNR--NVKIITLKSNLVSNFRKEF RLYKVREINDY 986 WP_007896501 917 SD KARFLRRQLVETRQITKHVAQLLDSRENSKSNQNKKLAR--NVKIITLKSKIVSDERKDF GLYKLREVNNY 987 EFR44625 869 SD KARFLRRQLVETRQITKHVAQLLDSRENSKSNQNKKLAR--NVKIITLKSKIVSDERKDF GLYKLREVNNY 939 WP_002897477 913 RD KVGFIRRQLVETQQITKNVAQILDARFNIEVKEKNQKIR--TVKIITLKSNLVSNFRKEF GLYKVREINNY 983 WP_002906454 913 RD KVGFIKRQLVETRQITKHVAQLLDTRFNIEVNEENQKIR--TVKIITLKSNLVSNFRKEF GLYKVREINDY 983 WP_009729476 914 LD KVGFIKRQLVETRQITKHVAQILDARFNKEVTEKDKKNR--TVKIITLKSNLVSNFRKEF ELYKVREINDY 984 CQR24647 913 ED KAGFIKRQLVETRQITKHVARILDERFNRDFDKNDKRIR--NVKIVILKSNLVSNERKEF GFYKVREINNF 983 WP_000066813 918 LD KVGFIKRQLVETRQITKHVAQFLDARFNKEVTEKDKKNR--NVKIITLKSNLVSNFRKEF GLYKVREINDY 988 WP_009754323 914 RD KVGFIKRQLVETRQITKHVARILDARFNIEVSEKNQKIR--SVKIITLKSNLVSNFRKEF KLYKVREINDY 984 WP_044674937 913 ED KARFIQRQLVETRQITKHVARILDTRENTKLDEAGNRIRdpKVNIITLKSNLVSQFRKDY QLYKVREINNY 985 WP_044676715 915 ED KARFIQRQLVETRQITKHVARILDTRENTKLDEAGNRIRdpKVNIITLKSNLVSQFRKDY QLYKVREINNY 987 WP_044680361 915 ED KARFIQRQLVETRQITKHVARILDTRENTKLDEAGNRIRdpKVNIITLKSNLVSQFRKDY QLYKVREINNY 987 WP_044681799 913 ED KARFIQRQLVETRQITKHVARILDTRENTKLDEAGNRIRdpKVNIITLKSNLVSQFRKDY QLYKVREINNY 985 WP_049533112 910 ND KAGFIKRQLVETRQITKHVAQVLDARFNAKHDENKKVIR--DVKIITLKSNLVSQFRKDF KFYKVREINDY 980 WP_029090905 891 RD KEGFIARQLVETRQITKHVTQLLQQEY-----------K-dTTKVFAIKATLVSGLRRKF EFIKNRNVNDY 951 WP_006506696 917 RD EERFINRQLVETRQITKNVIQIIEDHYST-------------TKVAAIRANLSHEFRVKN HIYKNRDINDY 976 AIT42264 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDERKDF QFYKVREINNY 981 WP_034440723 915 RD RQQFINRQLVETRQITKHVANLLSHHLNEK-----KEVG--EINIVLLKSALTSQFRKKF DFYKVREVNDY 980 AKQ21048 911 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDERKDF QFYKVREINNY 981 WP_004636532 916 AD RAHFLNRQLVETRQITKHVANLLDSQYNTAEEQ-----R---INIVLLKSSMTSRFRKEF KLYKVREINDY 980 WP_002364836 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNANSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_016631044 873 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 938 EMS75795 655 ED KARFIQRQLVETRQITKHVAAILDQYFN-QPEE-SK-NK--GIRIITLKSSLVSQFRKTF GINKVREINNH 722 WP_002373311 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_002378009 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_002407324 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_002413717 922 ED KAHFIQRQLVETRQITKNVAGILNQRYNANSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_010775580 924 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 989 WP_010818269 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_010824395 922 ED KAHFIQRQLVETRQITKNVAGILDQLYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_016622645 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_033624816 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_033625576 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_033789179 922 ED KAHFIQRQLVETRQITKNVAGILDQRYNAKSKE-----K--KVQIITLKASLTSQFRSIF GLYKVREVNDY 987 WP_002310644 919 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 988 WP_002312694 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNDPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_002314015 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_002320716 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_002330729 919 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 988 WP_002335161 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_002345439 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDTNEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_034867970 911 ED KARFIQRQLVETRQITKHVANILHQSFN-QEEEGTD-CD--GVQIITLKATLISQFRQTF GLYKVREINPH 979 WP_047937432 920 ED KAGFIKRQLVETRQITKHVAGILHHRFN-KAEDINEPIR--KVRIITLKSALVSQFRNRF GIYKVREINEY 989 WP_010720994 911 ED KARFIQRQLVETRQITKHVANILHQSFN-QEEEGTD-CD--GVQIITLKATLISQFRQTF GLYKVREINPH 979 WP_010737004 911 ED KARFIQRQLVETRQITKHVANILHQSFN-QEEEGTD-CD--GVQIITLKATLISQFRQTF GLYKVREINPH 979 WP_034700478 911 ED KARFIQRQLVETRQITKHVANILHQSFN-QEEEGTD-CD--GVQIITLKATLISQFRQTF GLYKVREINPH 979 WP_007209003 912 SD KAGELKRQLVETRQITKHVATILDSKFNE--DSNNRDVQ-----IITLKSALVSEFRKTF NLYKVREINDL 977 WP_023519017 905 AD KARFIQRQLVETRQITKHVANLLHQHFN-LPEEVSA-TE--KTSIITLKSTLTSQFRQMF DIYKVREINHH 973 WP_010770040 915 DD RAHFIKRQLVETRQITKHVARILDQRFNSQKDEEGKTIR--AVRVVTLKSSLTSQFRKQF AIHKVREINDY 985 WP_048604708 912 DD KAGFIHRQLVETRQITKNVARILHQRFNSEKDEEGNLIR--KVRIITLKSALTSQFRKNY GIYKIREINDY 982 WP_010750235 914 DD KARFIQRQLVETRQITKHVAAILHQYFN-QTQELEK-EK--DIRIITLKSSLVSQFRQVF GIHKVREINHH 982 AII16583 950 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDERKDF QFYKVREINNY 1020 WP_029073316 928 KD KERFINRQIVETRQITKHVAQIISNHYET-------------TKVVTVRADLSHAFRERY HIYKNRDINDF 987 WP_031589969 928 KD QERFINRQIVETRQITKHVAQIIDNHYEN-------------TKVVTVRADLSHQFRERY HIYKNRDINDF 987 KDA45870 903 KL KERFIERQLVETRQITKYVAQLLDQRLN--YDGNGVELD-eKIAIVTLKAQLASQFRSEF KLRKVRALNNL 972 WP_039099354 924 -D MKGFINRQLVETRQVIKLATNLLMEQYGED-----------NIELITVKSGLTHQMRTEF DFPKNRNLNNH 990 AKP02966 926 KD KLGFIHRQLVQTSQMVKGVANILNSMYK---NQGTTCIQ--------ARANLSTAFRKAL ELVKNRNINDF 999 WP_010991369 914 AD KARFIHRQLVETRQITKNVANILHQRFNYEKDDHGNTMK--QVRIVTLKSALVSQFRKQF QLYKVRDVNDY 984 WP_033838504 914 AD KARFIHRQLVETRQITKNVANILHQRFNYEKDDHGNTMK--QVRIVTLKSALVSQFRKQF QLYKVRDVNDY 984 EHN60060 917 AD KARFIHRQLVETRQITKNVANILHQRFNYEKDDHGNTMK--QVRIVTLKSALVSQFRKQF QLYKVRDVNDY 987 EFR89594 683 AD KARFIHRQLVETRQITKNVANILHQRFNYGKDDHGNTMK--QVRIVTLKSALVSQFRKQF QLYKVRGVNDY 753 WP_038409211 914 AD KANFIQRQLVETRQITKNVANILYQRFNCKQDENGNEVE--QVRIVTLKSTLVSQFRKQF QLYKVREVNDY 984 EFR95520 533 AD KANFIQRQLVETRQITKNVANILYQRFNCKQDENGNEVE--QVRIVTLKSTLVSQFRKQF QLYKVREVNDY 603 WP_003723650 914 AD KARFIHRQLVETRQITKNVANILYQRFNKEIDNHGNIME--QVRIVTLKSALVSQFRKQF QLYKVREVNGY 984 WP_003727705 914 AD KARFIHRQLVETRQITKNVANILHQRFNKEIDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 WP_003730785 914 AD KARFIHRQLVETRQITKNVANILHQRFNKEIDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 WP_003733029 914 AD KARFIHRQLVETRQITKNVANILHQRFNYKIDGNKDTME--TVRIVTLKSALVSQFRKQF QFYKVREVNDY 984 WP_003739838 914 AD KATFIHRQLVETRQITKNVANILHQRFNNETDNHGNNME--QVRIVMLKSALVSQFRKQF QLYKVREVNDY 984 WP_014601172 914 AD KARFIHRQLVETRQITKNVANILHQRFNNETDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 WP_023548323 914 AD KARFIHRQLVETRQITKNVANILHQRFNYKTDDNEDTME--PVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 WP_031665337 914 AD KARFIHRQLVETRQITKNVANILHQRFNKETDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 WP_031669209 914 AD KARFIHRQLVETRQITKNVANILHQRFNYKTDGNKDTME--TVRIVTLKSALVSQFRKQF QFYKVREVNDY 984 WP_033920898 914 AD KARFIHRQLVETRQITKNVANILHQRFNYKTDDNEDTME--PVRIVTLKSALVSQFRKQF QLYKVREVNDY 984 AKI42028 917 AD KARFIHRQLVETRQITKNVANILHQRFNNETDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 987 AKI50529 917 AD KARFIHRQLVETRQITKNVANILHQRFNYKTDDNEDTME--PVRIVTLKSALVSQFRKQF QLYKVREVNDY 987 EFR83390 362 AD KARFIHRQLVETRQITKNVANILHQRFNNETDNHGNTME--QVRIVTLKSALVSQFRKQF QLYKVREVNDY 432 WP_046323366 914 AD KARFIHRQLVETRQITKNVANILHQRFNCKKDESGNVIE--QVRIVTLKAALVSQFRKQF QLYKVREVNDY 984 AKE81011 927 LD KAGFIKRQLVETRQITKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 997 CUO82355 921 RD EERFINRQLVETRQITKNVTQIIEDHYST-------------TKVAAIRANLSHEFRVKN HIYKNRDINDY 980 WP_033162887 923 KD KERFINRQLVETRQIIKNVAVIINDHYTN-------------TNIVTVRAELSHQFRERY KIYKNRDINDF 982 AGZ01981 944 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 1014 AKA60242 911 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 AKS40380 911 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 981 4UN5_B 915 LD KAGFIKRQLVETRQIIKHVAQILDSRMNIKYDENDKLIR--EVKVITLKSKLVSDFRKDF QFYKVREINNY 985 WP_010922251 982

1051 WP_039695303 992 HHAHDAYLNAVVGTALLKKYPKL-ASEFVYGEYKKYDI S---SD------ KATAK--YfFYSNLM-NFFKTKVK 1058 WP_045635197 984 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGEYQKYDL SkdpKEV---EK ATEKY--F-FYSNLL-NFFKEEVH 1055 5AXW_A 703 HHAEDALI--------------IaNADFIFKEWKKLDK Nq-mFE----EK ETEQEykEiFITPHQiKHIKDFKD 771 WP_009880683 666 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDI S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 735 WP_010922251 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_011054416 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_011284745 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_011285506 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_011527619 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_012560673 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDI S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_014407541 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1050 WP_020905136 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_023080005 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1050 WP_023610282 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1050 WP_030125963 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_030126706 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_031488318 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_032460140 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDI S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_032461047 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDI S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_032462016 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_032462936 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_032464890 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_033888930 807 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 876 WP_038431314 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_038432938 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1050 WP_038434062 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 BAQ51233 893 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 962 KGE60162 157 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 226 KGE60856 -------------------------------------- ------------ ------------------------ WP_002989955 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_003030002 981 HHAHDAYLNAVVGNALLLKYPQL-EPEFVYGEYPKYN- S---YR---sRK SATEK--FlFYSNIL-RFFKKE-- 1041 WP_003065552 992 HHAHDAYLNAVVGTALLKKYPKL-ASEFVYGEYKKYDI S---SD------ KATAK--YfFYSNLM-NFFKRVIR 1058 WP_001040076 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040078 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGLYRRKK- L---SKI---VR ATRKM--F-FYSNLM-NMFKRVVR 1057 WP_001040080 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040081 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040083 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040085 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040087 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040088 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040089 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040090 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040091 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040092 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040094 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040095 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040096 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040097 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040098 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040099 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040100 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040104 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040105 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040106 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040107 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040108 987 HHAHDAYLNAVVAKAILTKYPQL-EREFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040109 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_001040110 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_015058523 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017643650 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017647151 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017648376 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017649527 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017771611 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_017771984 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 CFQ25032 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 CFV16040 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 KLJ37842 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 KLJ72361 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 KLL20707 1001 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1063 KLL42645 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_047207273 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_047209694 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_050198062 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_050201642 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_050204027 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_050881965 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_050886065 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 AHN30376 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 EAO78426 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 CCW42055 987 HHAHDAYLNAVVAKAILTKYPQL-EPEFVYGDYPKYN- S---YKT---RK ATEKL--F-FYSNIM-NFFKTKVT 1049 WP_003041502 981 HHAHDAYLNAVIGTALLKKYPKL-ASEFVYGEFKKYDV S---DK---eIG KATAK--YfFYSNLM-NFFKKEVK 1050 WP_037593752 982 HHAHDAYLNAVVGNALLLKYPQL-EPEFVYGEYPKYN- S---YR---sRK SATEK--FlFYSNIL-RFFKKE-- 1042 WP_049516684 982 HHAHDAYLNAVVGNALLLKYPQL-EPEFVYGEYPKYN- S---YR---sRK SATEK--FlFYSNIL-RFFKKE-- 1042 GAD46167 981 HHAHDAYLNAVVGNALLLKYPQL-EPEFVYGEYPKYN- S---YR---sRK SATEK--FlFYSNIL-RFFKKE-- 1041 WP_018363470 990 HHAHDAYLNAVVGTALLKKYPKL-APEFVYGEYKKYDV S---SDDhseMG KATAK--YfFYSNLM-NFFKRVIR 1062 WP_003043819 991 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEVK 1060 WP_006269658 981 HHAHDAYLNAVVGNALLLKYPQL-EPEFVYGEYPKYN- S---YR---sRK SATEK--FlFYSNIL-RFFKKE-- 1041 WP_048800889 981 HHAHDAYLNAVVGTALLKKYPKL-TSEFVYGEYKKYDV S---DND--eIG KATAK--YfFYSNLM-NFFKTEVK 1051 WP_012767106 981 HHAHDAYLNAVVGTALIKKYTKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_014612333 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_015017095 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_015057649 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_048327215 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_049519324 981 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKR--F-FYSNIM-NFFKTEIT 1050 WP_012515931 981 HHAHDAYLNAVVAKAILGKYPQL-APEFVYGDYPKYN- S---FKEr--QK ATQKM--L-FYSNIL-KFFKDQES 1043 WP_021320964 981 HHAHDAYLNAVVAKAILGKYPQL-APEFVYGDYPKYN- S---FKEr--QK ATQKT--L-FYSNIL-KFFKDQES 1043 WP_037581760 981 HHAHDAYLNAVVAKAILGKYPQL-APEFVYGDYPKYN- S---FKEr--QK ATQKT--L-FYSNIL-KFFKDQES 1043 WP_004232481 989 HHAHDAYLNAVVGTALLKKYPKL-APEFVYGEYKKYDV S---SDNhseLG KATAK--YfFYSNLM-NFFKTEVK 1061 WP_009854540 990 HHAHDAYLNAVVGTALLKKYPKL-ASEFVYGEYKKYDI S---SD------ KATAK--YfFYSNLM-NFFKTKVK 1056 WP_012962174 990 HHAHDAYLNAVVGTALLKKYPKL-APEFVYGEYKKYDI S---GD------ KATAK--YfFYSNLM-NFFKRVIR 1056 WP_039695303 992 HHAHDAYLNAVVGTALLKKYPKL-ASEFVYGEYKKYDI S---SD------ KATAK--YfFYSNLM-NFFKTKVK 1058 WP_014334983 989 HHAHDAYLNAVVGTALLKKYPKL-TPEFVYGEYKKYDV S---SDDyseMG KATAK--YfFYSNLM-NFFKTEVK 1061 WP_003099269 982 HHAQDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHYDL P---DSSl--GK ATTRM--F-FYSNLM-NFFKKEIK 1051 AHY15608 982 HHAQDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHYDL P---DSSl--GK ATTRM--F-FYSNLM-NFFKKEIK 1051 AHY17476 982 HHAQDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHYDL P---DSSl--GK ATTRM--F-FYSNLM-NFFKKEIK 1051 ESR09100 -------------------------------------- ------------ ------------------------ AGM98575 982 HHAQDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHYDL P---DSSl--GK ATTRM--F-FYSNLM-NFFKKEIK 1051 ALF27331 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_018372492 995 HHAHDAYLNAVVAQALLKVYPKF-ERELVYGSYVKESI ----FS----RK ATERM---rMYNNIL-KFISKD-- 1055 WP_045618028 985 HHAHDPYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL TkdpKEV---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 WP_045635197 984 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGEYQKYDL SkdpKEV---EK ATEKY--F-FYSNLL-NFFKEEVH 1055 WP_002263549 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002263887 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002264920 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002269043 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002269448 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002271977 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002272766 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HE---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002273241 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002275430 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HE---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002276448 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002277050 984 HHAHDAYLNAVVVKALLVKYPKL-EPEFVYGEYPKYN- S---YR---eRK ATQKM--F-FYSNIM-NMFKSKVK 1046 WP_002277364 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002279025 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HE---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002279859 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002280230 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002281696 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002282247 984 HHAHDAYLNAVVVKALLVKYPKL-EPEFVYGEYPKYN- S---YR---eRK ATQKM--F-FYSNIM-NMFKSKVK 1046 WP_002282906 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002283846 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002287255 982 HHTHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002288990 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002289641 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002290427 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002295753 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002296423 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002304487 996 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKG-- 1055 WP_002305844 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002307203 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002310390 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_002352408 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_012997688 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_014677909 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019312892 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019313659 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019314093 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019315370 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HE---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019803776 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_019805234 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_024783594 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_024784288 984 HHAHDAYLNAVVVKALLVKYPKL-EPEFVYGEYLKYN- S---YR---eRK ATQKM--F-FYSNIM-NMFKSKVK 1046 WP_024784666 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_024784894 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_024786433 984 HHAHDAYLNAVVVKALLVKYPKL-EPEFVYGEYPKYN- S---YR---eRK ATQKM--F-FYSNIM-NMFKSKVK 1046 WP_049473442 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HE---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 WP_049474547 982 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1041 EMC03581 975 HHAHDAYLNAVIGKALLGVYPQL-EPEFVYGDYPHFH- G---HK---eNK ATAKK--F-FYSNIM-NFFKKD-- 1034 WP_000428612 987 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SkdpKEI---EK ATEKY--F-FYSNLL-NFFKEEVH 1058 WP_000428613 985 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SrnpKEV---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 WP_049523028 980 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL TkdpKEI---EK ATEKY--F-FYSNLL-NFFKDKVY 1051 WP_003107102 951 HHAHDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHYDL S---DTSl--GK ATAKM--F-FYSNIM-NFFKKEVR 1020 WP_054279288 983 HHAHDAYLNAVVGTALLKMYPKL-ASEFVYGDYQKYDL S---GKAs--GH ATAKY--F-FYSNLM-NFFKSEVK 1052 WP_049531101 985 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SrdpKEI---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 WP_049538452 985 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SkdpKDI---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 WP_049549711 987 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKNDL SkdpKDI---EK ATEKY--F-FYSNLL-NFFKEEVH 1058 WP_007896501 988 HHAHDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHFDL S---DPSl--GK ATAKV--F-FYSNIM-NFFKEELS 1057 EFR44625 940 HHAHDAYLNAVVGTALLKKYPKL-EAEFVYGDYKHFDL S---DPSl--GK ATAKV--F-FYSNIM-NFFKEELS 1009 WP_002897477 984 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL FkpsKEI---EK ATEKY--F-FYSNLL-NFFKEEVL 1055 WP_002906454 984 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SkasNTI---DK ATEKY--F-FYSNLL-NFFKEKVR 1055 WP_009729476 985 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SkdpKEI---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 CQR24647 984 HHAHDAYLNAVVAKALLIRYPKL-EPEFVYGEYPKYN- S---YRE---RK ATEKM--F-FYSNIM-NMFKTTIK 1046 WP_000066813 989 HHAHDAYLNAVLAKAILKKYPKL-EPEFVYGDYQKYDL SrepKEV---EK ATQKY--F-FYSNLL-NFFKEEVH 1060 WP_009754323 985 HHAHDAYLNAVVAKAILKKYPKL-EPEFVYGDYQKYDL SkdpKEV---EK ATEKY--F-FYSNLL-NFFKEEVH 1056 WP_044674937 986 HHAHDAYLNAVVATALLKKYPQL-APEFVYGDYPKYN- S---YKS---RK ATEKV--L-FYSNIM-NFFRRVLV 1048 WP_044676715 988 HHAHDAYLNAVVATALLKKYPQL-APEFVYGDYPKYN- S---YKS---RK ATEKV--L-FYSNIM-NFFRRVLV 1050 WP_044680361 988 HHAHDAYLNAVVATALLKKYPQL-APEFVYGDYPKYN- S---YKS---RK ATEKV--L-FYSNIM-NFFRRVLV 1050 WP_044681799 986 HHAHDAYLNAVVATALLKKYPQL-APEFVYGDYPKYN- S---YKS---RK ATEKV--L-FYSNIM-NFFRRVLV 1048 WP_049533112 981 HHAHDAYLNAVIGTALLKKYPKL-ASEFVYGEFKKYDV S---DK---eIG KATAK--YfFYSNLM-NFFKKEVK 1050 WP_029090905 952 HHAQDAFLVAFLGTNITSNYPKI-EMEYLFKGYQHYLN ------Ev--GK AAKPKftF-IVENLS--------- 1007 WP_006506696 977 HHAHDAYIVALIGGFMRDRYPNMhDSKAVYSEYMKMFR ----NKNd--QK -----g---FVINSM-NYPY-EV- 1038 AIT42264 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_034440723 981 HHAHDAYLNGVIALKLLELYPYM-AKDLIYGKYSYHRK G---------DK ATQAK--Y-KMSNII-ERFSQDL- 1041 AKQ21048 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 WP_004636532 981 HHGHDAYLNAVVATTIMKVYPNL-KPQFVYGQYKKTSM ----FKE---EK ATARK--H-FYSNIT-KFFKKEKV 1042 WP_002364836 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_016631044 939 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 998 EMS75795 723 HHAHDAYLNGVVAIALLKKYPKL-EPEFVYGNYTKFNL ----AT---eNK ATAKK--E-FYSNIL-RFFEKE-- 782 WP_002373311 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQA ----FKE---NK ATAKT--I-IYTNLM-RFFTED-- 1047 WP_002378009 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_002407324 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_002413717 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_010775580 990 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQA ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1049 WP_010818269 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_010824395 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKT--I-IYTNLM-RFFTED-- 1047 WP_016622645 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQT ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_033624816 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQA ----FKE---NK AMAKA--I-IYTNLL-RFFTED-- 1047 WP_033625576 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQA ----FKE---NK ATAKA--I-IYTNLM-RFFTEV-- 1047 WP_033789179 988 HHGQDAYLNCVVATTLLKVYPNL-APEFVYGEYPKFQA ----FKE---NK ATAKA--I-IYTNLL-RFFTED-- 1047 WP_002310644 989 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1048 WP_002312694 990 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_002314015 990 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_002320716 990 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_002330729 989 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1048 WP_002335161 990 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_002345439 990 HHAHDAYLNGVVALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_034867970 980 HHAHDAYLNGFIANVLLKRYPKL-APEFVYGKYVKYSL ----AR---eNK ATAKK--E-FYSNIL-KFLESD-- 1039 WP_047937432 990 HHAHDAYLNGVIALALLKKYPQL-APEFVYGEYLKFNA ----HK---aNK ATVKK--E-FYSNIM-KFFESD-- 1049 WP_010720994 980 HHAHDAYLNGFIANVLLKRYPKL-APEFVYGKYVKYSL ----AR---eNK ATAKK--E-FYSNIL-KFLESD-- 1039 WP_010737004 980 HHAHDAYLNGFIANVLLKRYPKL-APEFVYGKYVKYSL ----AR---eNK ATAKK--E-FYSNIL-KFLESD-- 1039 WP_034700478 980 HHAHDAYLNGFIANVLLKRYPKL-APEFVYGKYVKYSL ----AR---eNK ATAKK--E-FYSNIL-KFLESD-- 1039 WP_007209003 978 HHAHDAYLNAVVALSLLRVYPQL-KPEFVYGEYGKNS- ----IHDg--NK ATIKK---qFYSNIT-RYFASK-- 1037 WP_023519017 974 HHAHDAYLNGVVAMTLLKKYPKL-APEFVYGSYIKGDI ----NQ---iNK ATAKK--E-FYSNIM-KFFESE-- 1033 WP_010770040 986 HHGHDAYLNGVVANSLLRVYPQL-QPEFVYGDYPKFNA ----YKA---NK ATAKK--Q-LYTNIM-KFFAED-- 1045 WP_048604708 983 HHAHDAYLNGVVATALLKIYPQL-EPEFVYGEFHRFNA ----FKE---NK ATAKK--Q-FYSNLM-EFSKSD-- 1042 WP_010750235 983 HHAHDAYLNAVVALALLKKYPRL-APEFVYGSFAKFHL ----VK---eNK ATAKK--E-FYSNIL-KFFEKE-- 1042 AII16583 1021 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1090 WP_029073316 988 HHAHDAYIATILGTYIGHRFESL-DAKYIYGEYQKIFR ----NKNk--DK ---KDg---FILNSM-RNLYADK- 1052 WP_031589969 988 HHAHDAYIATILGTYIGHRFESL-DAKYIYGEYKRIFR ----QKNk--GK ---NDg---FILNSM-RNIYADK- 1052 KDA45870 973 HHAHDAYLNAVVANLIMAKYPEL-EPEFVYGKYRKTK- ----FKGl--GK ATAKN---tLYANVL-YFLKENEV 1034 WP_039099354 991 HHAFDAYLTAFVGLYLLKRYPKL-KPYFVYGEYQKAS- ----QQ----DK ---RN--F----NFL-NGLKKD-- 1043 AKP02966 1000 HHAQDAYLASFLGTYRLRRFPTD-EMLLMNGEYNKFYG -----KElysKK -SRKN-gF-IISPLV--------- 1062 WP_010991369 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_033838504 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 EHN60060 988 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1047 EFR89594 754 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 813 WP_038409211 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-RFFAKE-- 1044 EFR95520 604 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-RFFAKE-- 663 WP_003723650 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_003727705 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_003730785 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_003733029 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFGW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_003739838 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_014601172 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFGQK-- 1044 WP_023548323 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_031665337 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_031669209 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 WP_033920898 985 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1044 AKI42028 988 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFGQK-- 1047 AKI50529 988 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 1047 EFR83390 433 HHAHDAYLNGVVANTLLKVYPQL-EPEFVYGEYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAQK-- 492 WP_046323366 985 HHAHDAYLNCVVANTLLKVYPQL-EPEFVYGDYHQFDW ----FKA---NK ATAKK--Q-FYTNIM-LFFAKK-- 1044 AKE81011 998 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1067 CUO82355 981 HHAHDAYIVALIGGFMRDRYPNMhDSKAVYSEYMKMFR ----NKNd--QK -----g---FVINSM-NYPY-EV- 1042 WP_033162887 983 HHAHDAYIACIVGQFMHQNFEHL-DAKIIYGQYK---- -----KNy--KK ---NYg---FILNSM-NHLQSDI- 1042 AGZ01981 1015 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1084 AKA60242 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 AKS40380 982 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1051 4UN5_B 986 HHAHDAYLNAVVGTALIKKYPKL-ESEFVYGDYKVYDV S---EQEi--GK ATAKY--F-FYSNIM-NFFKTEIT 1055 WP_010922251 1052

1114 WP_039695303 1059 YAD-GTVFERPIIE T-NAD-GE-IAWNKQIDFEKVRKVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1120 WP_045635197 1056 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFAIIKKVLS-LPQVNIVKKREVQT GGFSK ESIL-PKG- 1118 5AXW_A 772 YKYsHRVDKKPNRE VNNLN-GL---YDKDND--KLKKLINkSPEKLLMYHHDPQT --YQK KLIMeQYGd 852 WP_009880683 736 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 798 WP_010922251 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_011054416 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_011284745 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_011285506 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_011527619 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_012560673 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_014407541 1051 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1113 WP_020905136 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_023080005 1051 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1113 WP_023610282 1051 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1113 WP_030125963 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_030126706 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_031488318 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_032460140 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_032461047 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_032462016 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_032462936 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_032464890 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_033888930 877 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 939 WP_038431314 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_038432938 1051 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1113 WP_038434062 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 BAQ51233 963 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1025 KGE60162 227 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 289 KGE60856 1 ------------IE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 52 WP_002989955 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_003030002 1042 -----------DIQ T-NED-GE-IAWNKEKHIKILRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1093 WP_003065552 1059 YSN-GKVIVRPVVE Y-SKD-TEdIAWDKKSNFRTICKVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1121 WP_001040076 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040078 1058 LAD-GSIVVRPVIE TGRYM-GK-TAWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1120 WP_001040080 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040081 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040083 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040085 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040087 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040088 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040089 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040090 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040091 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040092 1050 LAD-ETVVVKDDIE VNNET-GE-IAWDKKKHFATVRKVLS-YPQVNIVKKTEVQT GGFSK ESIL-AHS- 1112 WP_001040094 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040095 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEVQT GGFSK ESIL-AHG- 1112 WP_001040096 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEVQT GGFSK ESIL-AHG- 1112 WP_001040097 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040098 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040099 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040100 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040104 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040105 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040106 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040107 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040108 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040109 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_001040110 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_015058523 1050 LAD-ETVVVKDDIE VNNET-GE-IAWDKKKHFATVRKVLS-YPQVNIVKKTEVQT GGFSK ESIL-AHS- 1112 WP_017643650 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_017647151 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_017648376 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_017649527 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_017771611 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_017771984 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 CFQ25032 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 CFV16040 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 KLJ37842 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 KLJ72361 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 KLL20707 1064 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1126 KLL42645 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_047207273 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_047209694 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_050198062 1050 LAD-GTVVIKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_050201642 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_050204027 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQVNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_050881965 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_050886065 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 AHN30376 1050 LAD-ETVVVKDDIE VNNET-GE-IAWDKKKHFATVRKVLS-YPQVNIVKKTEVQT GGFSK ESIL-AHS- 1112 EAO78426 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 CCW42055 1050 LAD-GTVVVKDDIE VNNDT-GE-IVWDKKKHFATVRKVLS-YPQNNIVKKTEIQT GGFSK ESIL-AHG- 1112 WP_003041502 1051 FAD-GTVVERPDIE T-SED-GE-IAWNKQTDFKIVRKVLS-YPQVNIVKKTEVQT HGLDR PSPK-PKP- 1122 WP_037593752 1043 -----------DIQ T-NED-GE-IAWNKEKHIKILRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1094 WP_049516684 1043 -----------DIQ T-NED-GE-IAWNKEKHIKILRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1094 GAD46167 1042 -----------DIQ T-NED-GE-IAWNKEKHIKILRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1093 WP_018363470 1063 YSN-GKVIVRPVVE Y-SKDtGE-IAWNKRTDFEKVRKVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1125 WP_003043819 1061 LAN-GEIRKRPLIE TNGET-GE-VVWNKEKDFATVRKVLA-MPQVNIVKKTEVQT GGFSK ESIL-SKR- 1123 WP_006269658 1042 -----------DIQ T-NED-GE-IAWNKEKHIKILRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1093 WP_048800889 1052 FAD-GTVVERPDIE T-SED-GE-IAWNKQTDFKIVRKVLS-YPQVNIVKKVEKQT GRFSK ESIL-PKG- 1113 WP_012767106 1051 LAN-GEIRKRPLIE TNEET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_014612333 1051 LAN-GEIRKRPLIE TNEET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_015017095 1051 LAN-GEIRKRPLIE TNEET-GE-IVWNKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_015057649 1051 LAN-GEIRKRPLIE TNEET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_048327215 1051 LAN-GEIRKRPLIE TNEET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_049519324 1051 LAN-GEIRKRPLIE TNEET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GALTN ESIY-ARG- 1113 WP_012515931 1044 L------------H VNSD--GE-EIWNANKHLPIIKNVLS-IPQVNIVKKTEVQT GGFYK ESIL-SKG- 1094 WP_021320964 1044 L------------H VNSD--GE-EIWNANKHLPIIKNVLS-IPQVNIVKKTEVQT GGFYK ESIL-SKG- 1094 WP_037581760 1044 L------------H VNSD--GE-EIWNANKHLPIIKNVLS-IPQVNIVKKTEVQT GGFYK ESIL-SKG- 1094 WP_004232481 1062 YAD-GRVFERPDIE T-NAD-GE-VVWNKQRDFNIVRKVLS-YPQVNIVKKVEVQT GGFSK ESIL-PKG- 1123 WP_009854540 1057 YAD-GTVFERPIIE T-NAD-GE-IAWNKQIDFEKVRKVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1118 WP_012962174 1057 YSN-GKVVVRPVIE C-SKDtGE-IAWNKQTDFEKVRRVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1119 WP_039695303 1059 YAD-GTVFERPIIE T-NAD-GE-IAWNKQIDFEKVRKVLS-YPQVNIVKKVETQT GGFSK ESIL-PKG- 1120 WP_014334983 1062 YAD-GRVFERPDIE T-NAD-GE-VVWNKQKDFDIVRKVLS-YPQVNIVKKVEAQT GGFSK ESIL-SKG- 1123 WP_003099269 1052 LAD-DTIFTRPQIE VNTET-GE-IVWDKVKDMQTIRKVMS-YPQVNIVMKTEVQT GGFSK ESIW-PKG- 1114 AHY15608 1052 LAD-DTIFTRPQIE VNTET-GE-IVWDKVKDMQTIRKVMS-YPQVNIVMKTEVQT GGFSK ESIW-PKG- 1114 AHY17476 1052 LAD-DTIFTRPQIE VNTET-GE-IVWDKVKDMQTIRKVMS-YPQVNIVMKTEVQT GGFSK ESIW-PKG- 1114 ESR09100 -------------- ----------------------------------------- ----- --------- AGM98575 1052 LAD-DTIFTRPQIE VNTET-GE-IVWDKVKDMQTIRKVMS-YPQVNIVMKTEVQT GGFSK ESIW-PKG- 1114 ALF27331 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_018372492 1056 --K----------K --DQEtGE-IVWDKKEIENIVKKVIY-SSPVNIVKKREEQS GALFK QSNM-AVGy 1108 WP_045618028 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKVLS-LPQVNIVKKTEEQT GGLFD NNIV-SKKk 1124 WP_045635197 1056 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFAIIKKVLS-LPQVNIVKKREVQT GGFSK ESIL-PKG- 1118 WP_002263549 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002263887 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002264920 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002269043 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002269448 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002271977 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002272766 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002273241 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002275430 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002276448 1042 -----------DVR T-DRN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002277050 1047 LAD-DQIVERPMIE VNDET-GE-IAWDKTKHITTVKKVLS-YPQVNIVKKVEEQT GGLFD -----PKS- 1111 WP_002277364 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002279025 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002279859 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002280230 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002281696 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002282247 1047 LAD-DQIVERPMIE VNDET-GE-IAWDKTKHITTVKKVLS-YPQVNIVKKVEEQT GGLFD -----PKS- 1111 WP_002282906 1042 -----------DVR I-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002283846 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002287255 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002288990 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002289641 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002290427 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFFK ESIL-PKG- 1093 WP_002295753 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002296423 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002304487 1056 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1107 WP_002305844 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002307203 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_002310390 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFFK ESIL-PKG- 1093 WP_002352408 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_012997688 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_014677909 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019312892 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019313659 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019314093 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019315370 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019803776 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_019805234 1042 -----------DVR T-DKN-GE-IIWKKDEYISNIKKVLS-YPQVNIVKKVEEQT GGFFK ESIL-PKG- 1093 WP_024783594 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_024784288 1047 LAD-DQIVERPMIE VNDET-GE-IAWDKTKHITTVKKVLS-YPQVNIVKKVEEQT GGLFD -----PKS- 1111 WP_024784666 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_024784894 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_024786433 1047 LAD-DQIVERPMIE VNDET-GE-IAWDKTKHITTVKKVLS-YPQVNIVKKVEEQT GGLFD -----PKS- 1111 WP_049473442 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 WP_049474547 1042 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1093 EMC03581 1035 -----------DVR T-DKN-GE-IIWKKDEHISNIKKVLS-YPQVNIVKKVEEQT GGFSK ESIL-PKG- 1086 WP_000428612 1059 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKVLS-LPQVNIVKKREVQT GGFSK ESIL-PKG- 1121 WP_000428613 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKVLS-YPQVNIVKKREVQT GGFSK ESIL-PKG- 1119 WP_049523028 1052 YAD-GTIIQRGNVE Y-SKDtGE-IAWNKKRDFAIVRKVLS-YPQVNIVKKTEEQT GGFSK ESIL-PKG- 1114 WP_003107102 1021 LAD-GTVITRPQIE TNTET-GE-IVWDKVKDIKTIRKVLS-IPQINVVKKTEVQT GGFSK ESIL-SKR- 1083 WP_054279288 1053 LAN-GNIIKRSPIE VNEET-GE-IVWDKIKDFGTVRKVLS-APQVNIVKKTEIQT GGFSN ETIL-SKG- 1115 WP_049531101 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEIDFATIRKILS-LSQVNIVKKTEEQT GGLFD NNIV-SKKk 1124 WP_049538452 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKILS-LPQVNIVKKTEEQT GGLFD NNIV-SKKk 1124 WP_049549711 1059 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKVLS-YPQVNIVKKTEEQT GGLFD NNIV-SKEk 1126 WP_007896501 1058 LAD-GTLMKRPVIE TNTET-GE-VVWDKVKDFKTIRKVLS-YPQVNIVKKTEIQS GAFSK ESVL-SKG- 1120 EFR44625 1010 LAD-GTLMKRPVIE TNTET-GE-VVWDKVKDFKTIRKVLS-YPQVNIVKKTEIQS GAFSK ESVL-SKG- 1072 WP_002897477 1056 YAD-GTIRKRENIE Y-SKDtGE-IAWDKEKDFATIKKVLS-YPQVNIVKKREVQT GGFSK ESIL-PKG- 1118 WP_002906454 1056 YAD-GTIKKRENIE Y-SNDtGE-IAWNKEKDFATIKKVLS-LPQVNIVKKTEEQT GGLFD NNIV-SKKk 1123 WP_009729476 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATIKKVLS-LPQVNIVKKREVQT GGFSK ESIL-PKG- 1119 CQR24647 1047 LAD-GRVVEKPVIE ANEET-GE-IAWDKTKHFANVKKVLS-YPQVSIVKKVEEQT GGFSK ESIL-PKG- 1109 WP_000066813 1061 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFATVKKVLS-LPQVNIVKKTEVQT GGFSK ESIL-PKG- 1123 WP_009754323 1057 YAD-GTIVKRENIE Y-SKDtGE-IAWNKEKDFVTIKKVLS-YPQVNIVKKREVQT GGFSK ESIL-PKG- 1119 WP_044674937 1049 YSKtGEVRIRPVIE VNKET-GE-IVWDKKSDFRTVRKVLS-YPQVNVVKKVEMQT GGFSK ESIL-QHG- 1112 WP_044676715 1051 YSKtGEVRIRPVIE VNKET-GE-IVWDKKSDFRTVRKVLS-YPQVNVVKKVEMQT GGFSK ESIL-QHG- 1114 WP_044680361 1051 YSKtGEVRIRPVIE VNKET-GE-IVWDKKSDFRTVRKVLS-YPQVNVVKKVEMQT GGFSK ESIL-QHG- 1114 WP_044681799 1049 YSKtGEVRIRPVIE VNKET-GE-IVWDKKSDFKTVRKVLS-YPQVNVVKKVEMQT GGFSK ESIL-QHG- 1112 WP_049533112 1051 FAD-GTVVERPDIE T-SED-GE-IAWNKQTDFKIVRKVLS-YPQVNIVKKTEVQT HGLDR PSPK-PKP- 1122 WP_029090905 1008 -KQ----------Q --NSTtGE-VKWNPEVDIAKLKRILN-FKQCNIVRKVEEQS GALFK ETIY-PVEe 1061 WP_006506696 1039 --D----------- ------GK-LIWNP-DLINEIKKCFY-YKDCYCTTKLDQKS GQLFN -TVL-SNDa 1084 AIT42264 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_034440723 1042 ------------LA --NPD-GE-IAWEKDKDLNTIRKVLS-SKQINIIKKAEEGK GRLFK ETIN-SRPs 1092 AKQ21048 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 WP_004636532 1043 -------------- VNEET-GE-ILWDTERHLSTIKRVLS-WKQMNIVKKVEKQK GQLWK ETIY-PKG- 1092 WP_002364836 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_016631044 999 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1049 EMS75795 783 --E----------Y SYDEN-GE-IFWDKARHIPQIKKVIS-SHQVNIVKKVEVQT GGFYK ETVN-PKG- 834 WP_002373311 1048 --E----------P RFTKD-SE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_002378009 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_002407324 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_002413717 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_010775580 1050 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1100 WP_010818269 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_010824395 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_016622645 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_033624816 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_033625576 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_033789179 1048 --E----------P RFTKD-GE-ILWSN-SYLKTIKKELN-YHQMNIVKKVEVQK GGFSK ESIK-PKG- 1098 WP_002310644 1049 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1100 WP_002312694 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_002314015 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_002320716 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_002330729 1049 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSE ETVE-PKK- 1100 WP_002335161 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_002345439 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_034867970 1040 --E----------P FCDEN-GE-IYWEKSHHLPRIKKVLS-SHQVNVVKKVEQQK GGFYK ETVN-SKE- 1091 WP_047937432 1050 --T----------P VCDEN-GE-IFWDKSKSIAQVKKVIN-HHHMNIVKKTEIQK GGFSK ETVE-PKK- 1101 WP_010720994 1040 --E----------P FCDEN-GE-IYWEKSHHLPRIKKVLS-SHQVNVVKKVEQQK GGFYK ETVN-SKE- 1091 WP_010737004 1040 --E----------P FCDEN-GE-IYWEKSHHLPRIKKVLS-SHQVNVVKKVEQQK GGFYK ETVN-SKE- 1091 WP_034700478 1040 --E----------P FCDEN-GE-IYWEKSHHLPRIKKVLS-SHQVNVVKKVEQQK GGFYK ETVN-SKE- 1091 WP_007209003 1038 --D----------- IINDD-GE-ILWNKQETIAQVIKTLG-MHQVNVVKKVEIQK GGFSK ESIQ-PKG- 1089 WP_023519017 1034 --E----------I ICDEQ-GE-VIWNKKRDLSTIKKTIG-AHQVNIVKKVEKQK GGFYK ETIN-SKA- 1085 WP_010770040 1046 --A----------V IIDEN-GE-ILWDK-KNIATVKKVMS-YPQMNIVKKPEIQT GSFSK ETIK-PKG- 1096 WP_048604708 1043 --K----------V IIDEN-GE-ILWNQ-KKIVTVKKVMN-YRQMNIVKKVEIQK GGFSK ESIL-PKG- 1093 WP_010750235 1043 --E----------Q FCDEN-GE-IFWDKRKHIQQIKKVIS-SHQVNIVKKVEVQT GSFYK ETVN-TKE- 1094 A1116583 1091 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1153 WP_029073316 1053 --D----------- ----T-GE-VVWDP-EWISRIKKCFY-YKDCFVTKKLEENN GSFFN -TVR-PNDe 1099 WP_031589969 1053 --D----------- ----T-GE-IVWDP-NYIDRIKKCFY-YKDCFVTKKLEENN GTFFN -TVL-PNDt 1099 KDA45870 1035 YPF----------- -----------WDKARDLPTIKRYLY-RAQVNKVRKAERQT GGFSD EMLV-PKS- 1078 WP_039099354 1044 -------------E LVDEN-TEaVIWNKESGLAYLNKIYQ-FKKILVTREVHENS GALFN QTLYaAKDd 1097 AKP02966 1063 --N-------GTTQ --DRNtGE-IIWNVG-FRDKILKIFN-YHQCNVTRKTEIKT GQFYD QTIYsPKNp 1118 WP_010991369 1045 --D----------R IIDEN-GE-ILWDK-KYLDTVKKVMS-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_033838504 1045 --D----------R IIDEN-GE-ILWDK-KYLDTVKKVMS-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 EHN60060 1048 --D----------R IIDEN-GE-ILWDK-KYLDTVKKVMS-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1098 EFR89594 814 --D----------R IIDEN-GE-ILWDK-KYLDTVKKVMS-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 864 WP_038409211 1045 --N----------Q IIDKN-GE-ILWDN-RYLDTIKKVLS-YRQMNIVKKTEIQK GEFSN ATVN-PKG- 1095 EFR95520 664 --N----------Q IIDKN-GE-ILWDN-RYLDTIKKVLS-YRQMNIVKKTEIQK GEFSN ATVN-PKG- 714 WP_003723650 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_003727705 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQINIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_003730785 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQINIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_003733029 1045 --D----------R IIDEN-GE-ILWDK-RYLETVKKVLG-YRQMNIVKKTEIQK GEFSN VTPN-PKG- 1095 WP_003739838 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_014601172 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_023548323 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLN-YRQMNIVKKTEIQK GEFSN QNPK-PRG- 1095 WP_031665337 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1095 WP_031669209 1045 --D----------R IIDEN-GE-ILWDK-RYLETVKKVLG-YRQMNIVKKTEIQK GEFSN VTPN-PKG- 1095 WP_033920898 1045 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLN-YRQMNIVKKTEIQK GEFSN QNPK-PRG- 1095 AKI42028 1048 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 1098 AKI 50529 1048 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLN-YRQMNIVKKTEIQK GEFSN QNPK-PRG- 1098 EFR83390 493 --E----------R IIDEN-GE-ILWDK-KYLETIKKVLD-YRQMNIVKKTEIQK GEFSK ATIK-PKG- 543 WP_046323366 1045 --D----------R IIDEN-GE-ILWDK-KYLDTIKKVLN-YRQMNIVKKTEIQK GEFSN ATAN-PKG- 1095 AKE81011 1068 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1130 CUO82355 1043 --D----------- ------GK-LIWNP-DLINEIKKCFY-YKDCYCITKLDQKS GQMFN -TVL-PNDa 1088 WP_033162887 1043 --D----------- ----T-GE-VMWDP-AKIGKIKSCFY-YKDVYVTKKLEQNS GTLFN -TVL-PNDa 1089 AGZ 01981 1085 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1147 AKA60242 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 AKS40380 1052 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1114 4UN5_B 1056 LAN-GEIRKRPLIE TNGET-GE-IVWDKGRDFATVRKVLS-MPQVNIVKKTEVQT GGFSK ESIL-PKR- 1118 WP_010922251 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_039695303 1121 --DSD KLIPRKIkKV-YW-DIKKYGGEDSPIVAYSV-FVVAD--VE--KGKAKKLKTVKELVGISIME RSFFEE 1185 WP_045635197 1119 --NSD KLIPRKT-KDILL-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KAAFEE 1183 5AXW_A 853 --EKN -LYKYYEeTGNYL---TKYSKKDNGPVIKKI------------KYYGNKLNAHLDITDDYPNS -VKLSL 912 WP_009880683 799 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELVGITIME RSSFEK 860 WP_010922251 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_011054416 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_011284745 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_011285506 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_011527619 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_012560673 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELVGITIME RSSFEK 1176 WP_014407541 1114 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1175 WP_020905136 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGLTIME RSSFEK 1176 WP_023080005 1114 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1175 WP_023610282 1114 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1175 WP_030125963 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_030126706 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_031488318 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_032460140 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELVGITIME RSSFEK 1176 WP_032461047 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELVGITIME RSSFEK 1176 WP_032462016 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_032462936 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_032464890 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_033888930 940 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1001 WP_038431314 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_038432938 1114 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1175 WP_038434062 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 BAQ51233 1026 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1087 KGE60162 290 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 351 KGE60856 53 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 114 WP_002989955 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_003030002 1094 --ESD KLIPRKT-KNSYW-NPKKYGGEDSPVVAYSI-LVFAD--VE--KGKSKKLRKVQDMVGITIME KKRFEK 1158 WP_003065552 1122 --DSD KLIPRKTkKA-YW-DIKKYGGEDSPIVAYSV-FVVAD--VE--KGKAKKLKTVKELVGISIME RSFFEE 1186 WP_001040076 1113 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040078 1121 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSKFEK 1185 WP_001040080 1113 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040081 1113 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040083 1113 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040085 1113 --NSD KLIPRKT-KDIYL-DPKKYGGEDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040087 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040088 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040089 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040090 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040091 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040092 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVLAD--IK--KGKAQKLKTVKELIGITIME RERFEK 1177 WP_001040094 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040095 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040096 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040097 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040098 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040099 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPKVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040100 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040104 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040105 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_001040106 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_001040107 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_001040108 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_001040109 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_001040110 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_015058523 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVLAD--IK--KGKAQKLKTVKELIGITIME RERFEK 1177 WP_017643650 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_017647151 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_017648376 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_017649527 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_017771611 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_017771984 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVAAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 CFQ25032 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 CFV16040 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 KLJ37842 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 KLJ72361 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 KLL20707 1127 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1191 KLL42645 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_047207273 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_047209694 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_050198062 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_050201642 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_050204027 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RFRFEK 1177 WP_050881965 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_050886065 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 AHN30376 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVLAD--IK--KGKAQKLKTVKELIGITIME RERFEK 1177 EAO78426 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 CCW42055 1113 --NSD KLIPRKT-KDIYL-DPKKYGGFDSPIVAYSV-LVVAD--IK--KGKAQKLKTVTELLGITIME RSRFEK 1177 WP_003041502 1123 --DSS ENLVGVK-RNL---DPKKYGGYAGISNSYAV-LVKAI--IE--KGVKKKETMVLEFQGISILD RITFEK 1185 WP_037593752 1095 --ESD KLIPRKT-KNSYW-NPKKYGGFDSPVVAYSI-LVFAD--VE--KGKSKKLRKVQDMVGITIME KKRFEK 1159 WP_049516684 1095 --ESD KLIPRKT-KNSYW-NPKKYGGFDSPVVAYSI-LVFAD--VE--KGKSKKLRKVQDMVGITIME KKRFEK 1159 GAD46167 1094 --ESD KLIPRKT-KNSYW-NPKKYGGFDSPVVAYSI-LVFAD--VE--KGKSKKLRKVQDMVGITIME KKRFEK 1158 WP_018363470 1126 --DSD KLIPRKTkKV-LW-EPKKYGGFDSPTVAYSV-LVVAD--VE--KGKIKKLKTVKELVGISIME RSFFEK 1190 WP_003043819 1124 --ESA KLIP----RKKGW-DIRKYGGFGSPTVAYSI-LVVAK--VE--KGKAKKLKSVKVLVGITIME KGSYEK 1185 WP_006269658 1094 --ESD KLIPRKT-KNSYW-DPKKYGGFDSPVVAYSI-LVFAD--VE--KGKSKKLRKVQDMVGITIME KKRFEK 1158 WP_048800889 1114 --DSD KLIARKTkEN-YW-DIKKYGGFDSPTVAYSV-LVVAD--IK--KGKAKKLKTVKELVGISIME RPFFEK 1178 WP_012767106 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKskVQ--DGKVKKIKTGKELIGMTLLD KLVFEK 1177 WP_014612333 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKskVQ--DGKVKKIKTGKELIGITLLD KLVFEK 1177 WP_015017095 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKskVQ--DGKVKKIKTGKELIGITLLD KLVFEK 1177 WP_015057649 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKskVQ--DGKVKKIKTGKELIGITLLD KLVFEK 1177 WP_048327215 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKckVQ--DGKVKKIKTGKELIGITLLD KLVFEK 1177 WP_049519324 1114 --SFD KLIS----RKHRF-ESSKYGGFGSPTVTYSV-LVVAKskVQ--DGKVKKIKTGKELIGITLLD KLVFEK 1177 WP_012515931 1095 --NSD KLIP----RKNNW-DTRKYGGFDSPTVAYSV-LVIAK--ME--KGKAKVLKPVKEMVGITIME RTAFEE 1156 WP_021320964 1095 --NSD KLIP----RKNNW-DIRKYGGFDSPTVAYSV-LVIAK--ME--KGKAKVLKPVKEMVGITIME RIAFEE 1156 WP_037581760 1095 --NSD KLIP----RKNNW-DIRKYGGFDSPTVAYSV-LVIAK--ME--KGKAKVLKPVKEMVGITIME RIAFEE 1156 WP_004232481 1124 --DSD KLIPRKTkKL-QW-ETQKYGGFDSPTVAYSV-LVVAD--VE--KGKIRKLKTVKELVGISIME RSSFEE 1188 WP_009854540 1119 --DSD KLIPRKIkKV-YW-DIKKYGGFDSPTVAYSV-FVVAD--VE--KGKAKKLKTVKELVGISIME RSFFEE 1183 WP_012962174 1120 --NSD KLIPRKTkKF-RW-DTPKYGGFDSPNIAYSV-FVIAD--VE--KGKAKKLKTVKELVGISIME RSSFEE 1184 WP_039695303 1121 --DSD KLIPRKIkKV-YW-DIKKYGGFDSPTVAYSV-FVVAD--VE--KGKAKKLKTVKELVGISIME RSFFEE 1185 WP_014334983 1124 --DSD KLIPRKIkKV-YW-NIKKYGGFDSPTVAYSV-LVVAD--IE--KGKAKKLKTVKELVGISIME RSFFEE 1188 WP_003099269 1115 --DSD KLIA----RKKSW-DPKKYGGFDSPIIAYSV-LVVAK--IA--KGKTQKLKTIKELVGIKIME QDEFEK 1176 AHY15608 1115 --DSD KLIA----RKKSW-DPKKYGGFDSPIIAYSV-LVVAK--IA--KGKTQKLKTIKELVGIKIME QDEFEK 1176 AHY17476 1115 --DSD KLIA----RKKSW-DPKKYGGFDSPIIAYSV-LVVAK--IA--KGKTQKLKTIKELVGIKIME QDEFEK 1176 ESR09100 1 ----- -------------------------------------------------------------ME QDEFEK 8 AGM98575 1115 --DSD KLIA----RKKSW-DPKKYGGFDSPIIAYSV-LVVAK--IA--KGKTQKLKTIKELVGIKIME QDEFEK 1176 AL F27331 1094 --NSD KLIPRKT-KKFYW-DTKKYGGFDSPIVAYSI-LVIAN--IE--KGKSKKLKLVKDLVGITIME RTIFEK 1158 WP_018372492 1109 ---NN KLIP----RKKDW-SVDKYGGFIEPAESYSLaIFYID--IN-----GKKPKKKSTIIAISRME KKDYEK 1167 WP_045618028 1125 vvDAS KLTPIKS-G---L-SPEKYGGYARPTIAYSV-LVIAD--IE--KGKAKKLKRIKEMVGITVQD KKKFEA 1188 WP_045635197 1119 --NSD KLIPRKT-KDILL-DTTKYGGFDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KAAFEE 1183 WP_002263549 1094 --NSD KLIPRKT-KKFYW-DIKKYGGFDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002263887 1094 --NSD KLIPRKT-KKFYW-DIKKYGGFDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002264920 1094 --DSD KLIPRKT-KKFYW-DIKKYGGFDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002269043 1094 --NSD KLIPRKT-KKFYW-DIKKYGGFDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002269448 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002271977 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002272766 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002273241 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002275430 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002276448 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002277050 1112 --PLE KLVPLKK----AL-NPEKYGGYQKPTTAYPI-LLIVD---------------TKQLIPISVMD KKRFEQ 1166 WP_002277364 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002279025 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002279859 1094 --DSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002280230 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002281696 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002282247 1112 --PLE KLVPLKK----AL-NPEKYGGYQKPTTAYPI-LLIVD---------------TKQLIPISVMD KKRFEQ 1166 WP_002282906 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002283846 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002287255 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002288990 1094 --NSY KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002289641 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002290427 1094 --DSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002295753 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002296423 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002304487 1108 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1172 WP_002305844 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002307203 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002310390 1094 --DSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_002352408 1094 --DSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_012997688 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_014677909 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019312892 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019313659 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019314093 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019315370 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019803776 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_019805234 1094 --DSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_024783594 1094 --NSD KLIPRKT-KKFYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KSKSKKLKTVKALVGVTIME KMTFER 1158 WP_024784288 1112 --PLE KLVPLKK----AL-NPEKYGGYQKPTTAYPI-LLIVD---------------TKQLIPISVMD KKRFEQ 1166 WP_024784666 1094 --NSD KLIPRKT-KKEYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_024784894 1094 --NSD KLIPRKT-KKEYW-DTKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_024786433 1112 --PLE KLVPLKK----AL-NPEKYGGYQKPTTAYPI-LLIVD---------------TKQLIPISVMD KKRFEQ 1166 WP_049473442 1094 --NSD KLIPRKT-KKEYW-DIKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 WP_049474547 1094 --NSD KLIPRKT-KKEYW-DIKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1158 EMC03581 1087 --NSD KLIPRKT-KKEYW-DIKKYGGEDSPIVAYSI-LVIAD--IE--KGKSKKLKTVKALVGVTIME KMTFER 1151 WP_000428612 1122 --NSD KLIPRKT-KDILW-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKRLKTVKILVGITIME KATFEK 1186 WP_000428613 1120 --NSD KLIPRKT-KDILW-ETTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KAAFEE 1184 WP_049523028 1115 --NSD KLIPRKT-KNVQL-DTTKYGGFDSPVIAYSI-LLVAD--VE--KGKSKKLKTVKSLIGITIME KVKFEA 1179 WP_003107102 1084 --DSD KLIP----RKNNW-DPKKYGGFGSPIIAYSV-LVVAK--VT--KGKSQKIKSVKELVGITIME QNEFEK 1145 WP_054279288 1116 --KSS KLIP----RKNKWrDTTKYGGENTPTVAYSV-LVVAK--VE--KGKAKKLKPVKELVGITIME RTKFEA 1178 WP_049531101 1125 vvDAS KLIPIKS-G---L-SPEKYGGYARPTIAYSV-LVIAD--IE--KGKAKKLKRIKEMVGITIQD KKKFEA 1188 WP_049538452 1125 vvDAS KLIPIKS-G---L-SPEKYGGYARPTIAYSV-LVIAD--IE--KGKIKKLKRIKEMIGITVQD KKIFES 1188 WP_049549711 1127 vvDAS KLIPIKS-G---L-SPEKYGGYARPTIAYSV-LVIAD--IE--KGKIKKLKRIKEMVGITIQD KKKFEA 1190 WP_007896501 1121 --NSD KLIE----RKKGW-DPKKYGGEDSPNTAYSI-FVVAK--VA--KRKAQKLKTVKEIVGITIME QAEYEK 1182 EFR44625 1073 --NSD KLIE----RKKGW-DPKKYGGEDSPNTAYSI-FVVAK--VA--KRKAQKLKTVKEIVGITIME QAEYEK 1134 WP_002897477 1119 --NSD KLIPRKT-KDILW-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KAAFEE 1183 WP_002906454 1124 vvDAS KLIPIKS-S---L-SPEKYGGYARPTIAYSV-LVIAD--IEkgKGKAKKLKRIKEIVGITIQD KKKFES 1189 WP_009729476 1120 --NSD KLIPRKT-KDILW-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KDAFEK 1184 CQR24647 1110 --GSD KLIARKT-KNNYL-STQKYGGFDSPIVAYSI-MFVAD--IE--KGKSKRLKTVKEMIGITIME RSRFES 1174 WP_000066813 1124 --NSD KLIPRKT-KEILW-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KATFEK 1188 WP_009754323 1120 --NSD KLIPRKT-KDILW-DTTKYGGEDSPVIAYSI-LLIAD--IE--KGKAKKLKTVKILVGITIME KAAFEK 1184 WP_044674937 1113 --DSD KLIPRKT-EKFYL-DIKKYGGEDSPTIAYSV-LLIAD--IE--KGKAKKLKRVKELIGITIME RMAFEK 1177 WP_044676715 1115 --DSD KLIPRKT-EKFYL-DIKKYGGEDSPTIAYSV-LLIAD--IE--KGKAKKLKRVKELIGITIME RMAFEK 1179 WP_044680361 1115 --DSD KLIPRKT-EKFYL-DIKKYGGEDSPTIAYSV-LLIAD--IE--KGKAKKLKRVKELIGITIME RMAFEK 1179 WP_044681799 1113 --DSD KLIPRKT-EKFYL-DIKKYGGEDSPTIAYSV-LLIAD--IE--KGKAKKLKRVKELIGITIME RMAFEK 1177 WP_049533112 1123 --DSS ENLVGVK-RNL---DPKKYGGYAGISNSYAV-LVKAI--IE--KGVKKKETMVLEFQGISILD RITFEK 1185 WP_029090905 1062 --SSS KTIP----LKKHL-DTAIYGGYTAVNYASYA---LIQ--FK----KGRKLK--REIIGIPLAV QTRIDN 1117 WP_006506696 1085 haDKG AVVP---vNKNRS-DVHKYGGFSG--LQYTI----VA--IEgqKKKGKKTELVKKISGVPLHL KAASIN 1149 AIT42264 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_034440723 1093 k-KTE KRIP----IKNNL-DPNIYGGYIEEKMAYYI----AInyLE--NGKTKK-----AIVGISIKD KKDFEG 1149 AKQ21048 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 WP_004636532 1093 --DSS KLIP----VKEGM-DPQKYGGLSQVSEAFAV-VIT----HE--KGKKKQLK--SDLISIPIVD QKAYEQ 1150 WP_002364836 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPIVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_016631044 1050 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1107 EMS75795 835 --KPD KLIQ----RKAGW-DVSKYGGFGSPVVAYAV-AFI----YE--KGKAR--KKAKAIEGITIMK QSLFEQ 892 WP_002373311 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_002378009 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPIVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_002407324 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPIVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_002413717 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_010775580 1101 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1158 WP_010818269 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPIVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_010824395 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_016622645 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPIVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_033624816 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_033625576 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTKFEQ 1156 WP_033789179 1099 --PSN KLIP----VKNGL-DPQKYGGFDSPVVAYTV-LF--T--HE--KGK-KPL-IKQEILGITIME KTRFEQ 1156 WP_002310644 1101 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1158 WP_002312694 1102 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_002314015 1102 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_002320716 1102 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_002330729 1101 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1158 WP_002335161 1102 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_002345439 1102 --DSS KLLP----RKNNW-DPTKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_034867970 1092 --KPD KLIE----RKNNW-DVIKYGGFGSPVIAYAI-AFV----YA--KGKTQ--KKTRAIEGITIME QAAFEK 1149 WP_047937432 1102 --DSS KLLP----RKNNW-DPAKYGGLGSPNVAYTV-AFT----YE--KGKAR--KRTNALEGITIME REAFEQ 1159 WP_010720994 1092 --KPD KLIE----RKNNW-DVIKYGGFGSPVIAYAI-AFV----YA--KGKTQ--KKIKAIEGITIME QAAFEK 1149 WP_010737004 1092 --KPD KLIE----RKNNW-DVIKYGGFGSPVIAYAI-AFV----YA--KGKTQ--KKTRAIEGITIME QAAFEK 1149 WP_034700478 1092 --KPD KLIE----RKNNW-DVIKYGGFGSPVIAYAI-AFV----YA--KGKTQ--KKTRAIEGITIME QAAFEK 1149 WP_007209003 1090 --ESQ KLIR----RKQQW-NTKKYGGFDSPVVAYAI---LLS--FD--KGK-RKARSFK-IVGITIQD RESFEG 1147 WP_023519017 1086 --NPE KLIP----RKASL-DPLKYGGYGSPLVAYTV-IFI----FE--KGKQK--KVIKGIEGITVME QLRFEQ 1143 WP_010770040 1097 --DSD KLIS----RKTNW-SPKLYGGFDSPQVAYSV-II--T--YE--KGK-KKVRA-KAIVGITIME QSLFKK 1154 WP_048604708 1094 --DSD KLIS----RKKEW-DTTKYGGFDSPNVAYSV-VI--R--YE--KGK-TRKLV-KTIVGITIME RAAFEK 1151 WP_010750235 1095 --KPD KLIK----RKNNW-DVTKYGGFGSPVVAYAV-VFT----YE--KGKNH--KKAKAIEGITIME QALFEK 1152 AII16583 1154 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1215 WP_029073316 1100 hsEKG AKVP---vNKLRS-NVHKYGGFEG--LKYSI----VA--IKgkKKKGKKIIDVNKLVGIPLMY KNVDDE 1164 WP_031589969 1100 nsDKD ATVP---vNKYRS-NVNKYGGFSG--VNSFI----VA--IKgkKKKGKKVIEVNKLIGIPLMY KNADEE 1164 KDA45870 1079 --DSG KLLP----RKEGL-DPVKYGGYAKAVESYAV-LITAD-eVK--KGKTKKVKT---LVNIPIID SKKYEA 1138 WP_039099354 1098 k-ASG QLIPAKQdRPTAL-----YGGYSGKTVAYMC---IVR--IKnkKGDLYKVCGVETSWLAQLKQ KKAFLK 1170 AKP02966 1119 k---- KLIA----QKKDM-DPNIYGGFSGDNKSSIT---IVK--ID-----NNKIKPVA--IPIRLIN ----DK 1172 WP_010991369 1096 --NSS KLIP----RKTNW-DPMKYGGLDSPNMAYAV-VI--E--YA--KGK-NKLVFEKKIIRVTIME RKAFEK 1154 WP_033838504 1096 --NSS KLIS----RKTNW-DPMKYGGLDSPNMAYAV-VI--E--YA--KGK-NKLVFEKKIIRVTIME RKAFEK 1154 EHN60060 1099 --NSS KLIS----RKTNW-DPMKYGGLDSPNMAYAV-VI--E--YA--KGK-NKLVFEKKIIRVTIME RKAFEK 1157 EFR89594 865 --NSS KLIP----RKTNW-DPMKYGGLDSPNMAYAV-VI--E--YA--KGK-NKLVFEKKIIRVTIME RKAFEK 923 WP_038409211 1096 --NSS KLIS----RKADW-NPIKYGGFDGSNMAYSI-VI--E--YE--KRK-KKTVIKKELIQINIME RVAFEK 1154 EFR95520 715 --NSS KLIS----RKADW-NPIKYGGFDGSNMAYSI-VI--E--YE--KRK-KKTVIKKELIQINIME RVAFEK 773 WP_003723650 1096 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKIVIEKKLIQINIME RKMFEK 1154 WP_003727705 1096 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKIVIEKKLIQINIME RKMFEK 1154 WP_003730785 1096 --NSS KLIP----RKENW-DPVKYGGLDSPNMAYAV-II--E--HA--KGK-KKIVIEKKLIQINIME RKMFEK 1154 WP_003733029 1096 --KSN KLIP----RKKDW-DPIKYGGFDGSKMAYAI-II--E--YE--KQK-RKVRIEKKLIQINIME REAFEK 1154 WP_003739838 1096 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKVVFEKKIIRITIME RKAFEK 1154 WP_014601172 1096 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKLIFEKKIIRITIME RKMFEK 1154 WP_023548323 1096 --DSS KLIP----KKTNL-NPIKYGGFEGSNMAYAI-II--E--HE--KRK-KKVTIEKKLIQINIME RKAFEK 1154 WP_031665337 1096 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KRIVIEKKLIQINIME RKMFEK 1154 WP_031669209 1096 --KSN KLIP----RKKDW-DPIKYGGFDGSKMAYAI-II--E--YE--KQK-RKVRIEKKLIQINIME REAFEK 1154 WP_033920898 1096 --DSS KLIP----KKTNL-NPIKYGGFEGSNMAYAI-II--E--HE--KRK-KKVTIEKKLIQINIME RKAFEK 1154 AKI42028 1099 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKLIFEKKIIRITIME RKMFEK 1157 AKI50529 1099 --DSS KLIP----KKTNL-NPIKYGGFEGSNMAYAI-II--E--HE--KRK-KKVTIEKKLIQINIME RKAFEK 1157 EFR83390 544 --NSS KLIP----RKENW-DPMKYGGLDSPNMAYAV-II--E--HA--KGK-KKIVIEKKLIQINIME RKMFEK 602 WP_046323366 1096 --NSS KLIP----RKADW-DPIKYGGFDGSNMAYAI-VI--E--HE--KRK-KKTVIKKELIQINIME RTAFEK 1154 AKE81011 1131 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1192 CUO82355 1089 hsAKG AVIP---vNKNRK-DVNKYGGFSG--LQYVI----AA--IEgtKKKGKKLVKVRKLSGIPLYL KQADIK 1153 WP_033162887 1090 hsEKG ATVP---lNKYRA-DVHKYGGFGN--VQSII----VA--IEgkKKKGKKLIDVRKLISIPLHL KNAPVE 1154 AGZ01981 1148 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1209 AKA60242 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 AKS40380 1115 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1176 4UN5_B 1119 --NSD KLIA----RKKDW-DPKKYGGFDSPIVAYSV-LVVAK--VE--KGKSKKLKSVKELLGITIME RSSFEK 1180 WP_010922251 1177

1239 WP_039695303 1186 NPV---EFLE---NKGYHN--I-REDKLIK--LPKYSLFE---FEGGRRRLLAS ASELQKGNEMVLPGYLVELLYHA 1248 WP_045635197 1184 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LENGRRRLLAS AKELQKGNEIVLPVYLTTLLYHS 1246 5AXW_A 913 KPYrfdVYLD---NGVYKFvtV-KNLDVIK----KENYYE---VNSKAYEEAKK -KKISNQAEFIASFYNNDLIKIN 978 WP_009880683 861 DPV---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 923 WP_010922251 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_011054416 1177 DPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WPO11284745 1177 NPI---DFLE---AKGYKE--V-RKDLIVK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_011285506 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_011527619 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_012560673 1177 DPV---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_014407541 1176 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1238 WP_020905136 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_023080005 1176 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1238 WP_023610282 1176 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1238 WP_030125963 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_030126706 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_031488318 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_032460140 1177 DPV---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_032461047 1177 DPV---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_032462016 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_032462936 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_032464890 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_033888930 1002 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1064 WP_038431314 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_038432938 1176 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1238 WP_038434062 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 BAQ51233 1088 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1150 KGE60162 352 DPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 414 KGE60856 115 DPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 177 WP_002989955 1177 NPI---DFLE---AKGYKE--V-RKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_003030002 1159 HPV---DFLE---QRGYRN--V-RLEKIIK--LPKYSLFE---LENKRRRLLAS ARELQKGNELVIPQRFTTLLYHS 1221 WP_003065552 1187 NPV---EFLE---NKGYHN--I-REDKLIK--LPKYSLFE---FEGGKRRLLAS ASELQKGNEMVIPGHLVKLLYHA 1249 WP_001040076 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040078 1186 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1248 WP_001040080 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040081 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGETIDRLQKGNELALPTQFMKFLYLA 1240 WP_001040083 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040085 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040087 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040088 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040089 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040090 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040091 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040092 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040094 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040095 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040096 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040097 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS ADELQKGNELALPTQFMKFLYLA 1240 WP_001040098 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040099 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040100 1178 NPS---AFLE---SKGYLD--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040104 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040105 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_001040106 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040107 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040108 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040109 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_001040110 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_015058523 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_017643650 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS ADELQKGNELALPTQFMKFLYLA 1240 WP_017647151 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_017648376 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_017649527 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_017771611 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_017771984 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 CFQ25032 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 CFV16040 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 KLJ37842 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 KLJ72361 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 KLL20707 1192 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1254 KLL42645 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_047207273 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_047209694 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_050198062 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_050201642 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_050204027 1178 NPS---AFLE---SKGYLN--I-RDDKLMI--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 WP_050881965 1178 NLS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_050886065 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 AHN30376 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQYMKFLYLA 1240 EAO78426 1178 NPS---AFLE---SKGYLN--I-RADKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 CCW42055 1178 NPS---AFLE---SKGYLN--I-RTDKLII--LPKYSLFE---LENGRRRLLAS AGELQKGNELALPTQFMKFLYLA 1240 WP_003041502 1186 DKR---AFLL---GKGYKD--I-K--KIIE--LPKYSLFE---LKDGSRRMLAS RGEIHKGNELFVPQKFTTLLYHA 1253 WP_037593752 1160 NPV---DFLE---QRGYRN--V-RLEKIIK--LPKYSLFE---LENKRRRLLAS ARELQKGNELVIPQRFTTLLYHS 1222 WP_049516684 1160 HPV---DFLE---QRGYRN--V-RLEKIIK--LPKYSLFE---LENKRRRLLAS ARELQKGNELVIPQRFTTLLYHS 1222 GAD46167 1159 NPV---DFLE---QRGYRN--V-RLEKIIK--LPKYSLFE---LENKRRRLLAS ARELQKGNELVIPQRFTTLLYHS 1221 WP_018363470 1191 NPV---EFLK---NKGYQN--V-QEDKLMK--LPKYSLFE---FEGGRRRLLAS ATELQKGNEIMLSAHLVALLYHA 1253 WP_003043819 1186 DPI---GFLE---AKGYKD--I-KKELIFK--LPKYSLFE---LENGRRRMLAS --ELQKANELVLPQHLVRLLYYT 1248 WP_006269658 1159 NPV---DFLE---QRGYRN--V-RLEKIIK--LPKYSLFE---LENKRRRLLAS AKELQKGNELVIPQRFTTLLYHS 1221 WP_048800889 1179 NPI---MFLE---SKGYRN--I-QKDKLIK--LPKYSLFE---FEGGRRRLLAS AVELQKGNEMVLPQYLNNLLYHA 1241 WP_012767106 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_014612333 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_015017095 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_015057649 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_048327215 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_049519324 1178 NPL---KFIE---DKGYGN--V-QIDKCIK--LPKYSLFE---FENGTRRMLAS RGDLQKANEMFLPAKLVTLLY-- 1245 WP_012515931 1157 NPV---VFLE---ARGYRE--I-QEHLIIK--LPKYSLFE---LENGRRRLLAS -SELQKGNELFLPVDYMTFLYLA 1219 WP_021320964 1157 NPV---VFLE---AKGYRE--I-QEHLIIK--LPKYSLFE---LENGRRRLLAS -SELQKGNELFLPVDYMTFLYLA 1219 WP_037581760 1157 NPV---VFLE---AKGYRE--I-QEHLIIK--LPKYSLFE---LENGRRRLLAS -SELQKGNELFLPVDYMTFLYLA 1219 WP_004232481 1189 NPV---SFLE---KKGYHN--V-QEDKLIK--LPKYSLFE---FEGGRRRLLAS ATELQKGNEVVLPQYMVNLLYHS 1251 WP_009854540 1184 NPV---EFLE---NKGYHN--I-REDKLIK--LPKYSLFE---FEGGRRRLLAS ASELQKGNEMVLPGYLVELLYHA 1246 WP_012962174 1185 NPV---VFLE---KKGYQN--V-QEDNLIK--LPKYSLFE---FEGGRRRLLAS ASELQKGNEVVLSRHLVELLYHA 1247 WP_039695303 1186 NPV---EFLE---NKGYHN--I-REDKLIK--LPKYSLFE---FEGGRRRLLAS ASELQKGNEMVLPGYLVELLYHA 1248 WP_014334983 1189 NPV---SFLE---KKGYHN--V-QEDKLIK--LPKYSLFE---FEGGRRRLLAS ATELQKGNEVMLPAHLVELLYHA 1251 WP_003099269 1177 DPI---AFLE---KKGYQD--I-QTSSIIK--LPKYSLFE---LENGRKRLLAS --ELQKGNELALPNKYVKFLYLA 1239 AHY15608 1177 DPI---AFLE---KKGYQD--I-QTSSIIK--LPKYSLFE---LENGRKRLLAS --ELQKGNELALPNKYVKFLYLA 1239 AHY17476 1177 DPI---AFLE---KKGYQD--I-QTSSIIK--LPKYSLFE---LENGRKRLLAS --ELQKGNELALPNKYVKFLYLA 1239 ESR09100 9 DPI---AFLE---KKGYQD--I-QTSSIIK--LPKYSLFE---LENGRKRLLAS -KELQKGNELALPNKYVKFLYLA 71 AGM98575 1177 DPI---AFLE---KKGYQD--I-QTSSIIK--LPKYSLFE---LENGRKRLLAS --ELQKGNELALPNKYVKFLYLA 1239 ALF27331 1159 NPV---AFLE---RKGYRN--V-QEENIVK--LPKYSLFE---LENGRRRLLAS ARELQKGNEIVLPNHLGTMLYHA 1221 WP_018372492 1168 EPEr---FLA---QKGFER--V-EKT--IK--LPKYSLFE---MEKGRRRLLAS SGELQKGNQVLLPEHLIRLLSYA 1228 WP_045618028 1189 NPI---AYLE---ECGYKN--I-NPNLIIK--LPKYSLFE---FNNGQRRLLAS SIELQKGNELIVPYHFTALLYHA 1251 WP_045635197 1184 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LENGRRRLLAS AKELQKGNEIVLPVYLTTLLYHS 1246 WP_002263549 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002263887 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002264920 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002269043 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002269448 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002271977 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002272766 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002273241 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002275430 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002276448 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002277050 1167 NPV---KFLK---DKGYQQ--I-EKNNFVK--LPKYTLVD---IGNGIKRLWAS SKEVHKGNQLVVSKKSQDLLYHA 1229 WP_002277364 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002279025 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002279859 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002280230 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002281696 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002282247 1167 NPV---KFLK---DKGYQQ--I-EKNNFVK--LPKYTLVD---IGNGIKRLWAS SKEVHKGNQLVVSKKSQDLLYHA 1229 WP_002282906 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002283846 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002287255 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002288990 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002289641 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002290427 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002295753 1159 DPI---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002296423 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002304487 1173 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLETLLYHA 1235 WP_002305844 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002307203 1159 DPI---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002310390 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_002352408 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPDHLGTLLYHA 1221 WP_012997688 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_014677909 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019312892 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019313659 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019314093 1159 DPI---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019315370 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019803776 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_019805234 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_024783594 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_024784288 1167 NPV---KFLK---DKGYQQ--I-EKNNFVK--LPKYTLVD---IGNGIKRLWAS SKEVHKGNQLVVSKKSQDLLYHA 1229 WP_024784666 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_024784894 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_024786433 1167 NPV---KFLK---DKGYQQ--I-EKNNFVK--LPKYTLVD---IGNGIKRLWAS SKEVHKGNQLVVSKKSQDLLYHA 1229 WP_049473442 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 WP_049474547 1159 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1221 EMC03581 1152 DPV---AFLE---RKGYRN--V-QEENIIK--LPKYSLFK---LENGRKRLLAS ARELQKGNEIVLPNHLGTLLYHA 1214 WP_000428612 1187 SPI---AFLE---NKGYHN--V-RKENILC--LPKYSLFE---LKNGRRRMLAS AKELQKGNEIVLPVHLTTLLYHA 1249 WP_000428613 1185 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LENGRRRLLAS AKELQKGNEIVLPVYLTTLLYHS 1247 WP_049523028 1180 NPV---AFLE---GKGYQN--V-VEENIIR--LPKYSLFE---LENGRRRMLAS AKELQKGNEMVLPSYLIALLYHA 1242 WP_003107102 1146 DRI---TFLE---KKGYQD--I-QESLIIK--LPKFSLFE---LENGRKRLLAS --ELQKGNELSLPNKYIQFLYLA 1208 WP_054279288 1179 NPI---AFLE---SKGYHD--I-QEHLMIT--LPKYSLFE---LENGRRRLLAS --ELQKGNEMVLPQHLVTFLYRV 1241 WP_049531101 1189 NPT---AYLE---EYGYKN--I-NPNLIIK--LPKYSLFK---FNDGQRRLLAS SIELQKGNELILPYHFTTLLYHA 1251 WP_049538452 1189 NPI---AYLE---ECGYKN--I-NPNLIIK--LPKYSLFE---FNGGQRRLLAS SIELQKGNELILPYHFTALLYHT 1251 WP_049549711 1191 NPI---AYLE---ECGYKN--I-NPNLIIK--LPKYSLFE---FNGGQRRLLAS SIELQKGNELILPYHFTALLYHA 1253 WP_007896501 1183 DNI---AFLE---KKGYQD--I-QEKLLIK--LPKYSLFE---LENGRRRLLAS --EFQKGNELALSGKYMKFLYLA 1245 EFR44625 1135 DNI---AFLE---KKGYQD--I-QEKLLIK--LPKYSLFE---LENGRRRLLAS --EFQKGNELALSGKYMKFLYLA 1197 WP_002897477 1184 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LENGRRRLLAS AKELQKGNEIVLPVCLTTLLYHS 1246 WP_002906454 1190 NPV---TYLE---ECGYKN--I-NSNLIIK--LPKYSLFE---FNDGQRRLLAS SIELQKGNELILPYHLTALLYHA 1252 WP_009729476 1185 NPI---AFLE---NKGYHN--V-CKENILC--LPKYSLFE---LENGRRRLLAS AKELQKCNEIVLPVYLTTLLYHS 1247 CQR24647 1175 NSV---TFLE---EKGYRN--I-RENTIIK--FPKYSLFE---LENGRRRLLAS AIELQKGNEMFLPQQFVNLLYHA 1237 WP_000066813 1189 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LESGRRRMLAS AKELQKGNEIVLPVYLTTLLYHS 1251 WP_009754323 1185 NPI---TFLE---NKGYHN--V-RKENILC--LPKYSLFE---LENGRRRLLAS AKELQKGNEIVLPVYLTTLLYHS 1247 WP_044674937 1178 NPI---EFLE---HKGYKN--I-LEKNIIK--LPKYSLFE---LENGRRRLLAS AKELQKGNEMILPPHLVTLLYHS 1240 WP_044676715 1180 NPI---EFLE---HKGYKN--I-LEKNIIK--LPKYSLFE---LENGRRRLLAS AKELQKGNEMILPPHLVTLLYHS 1242 WP_044680361 1180 NPI---EFLE---HKGYKN--I-LEKNIIK--LPKYSLFE---LENGRRRLLAS AKELQKGNEMILPPHLVTLLYHS 1242 WP_044681799 1178 NPI---EFLE---HKGYKN--I-LEKNIIK--LPKYSLFE---LENGRRRLLAS AKELQKGNEMILPPHLVTLLYHS 1240 WP_049533112 1186 DKR---AFLL---GKGYKD--I-K--KIIE--LPKYSLFE---LKDGSRRMLAS RGEIHKGNELFVPQKFTTLLYHA 1253 WP_029090905 1118 SETslgAYIA---EQIKSE--VeILN----grILKYQLIS----NNGNRLYIAG -SERHNARQLIVSDEAAKVIWLI 1181 WP_006506696 1150 EKI---NYIE--eKEGLSD--VrIIK---Dn-IPVNQMIEm----DGGEYLLTS --EYVNARQLVLNEKQCALIADI 1211 AIT42264 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_034440723 1150 QTT---EYLG---KIGFNK--AsIIN---S--FKNYTLFE---LENGSRRMIVG KGELQKGNQMYLPQNLLEFVYHL 1217 AKQ21048 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 WP_004636532 1151 HPT---AYLE---EAGYNN--P-TV--LHE--LFKYQLFE---LEDGSRRMIAS AKEFQKGNQMVLPLELVELLYHA 1211 WP_002364836 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_016631044 1108 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1168 EMS75795 893 DPI---GFLS---NKGYSN--V-TKF--IK--LSKYTLYE---LENGRRRMVAS -KEAQKANSFILPEKLVTLLYHA 953 WP_002373311 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_002378009 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYQ---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_002407324 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_002413717 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_010775580 1159 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1219 WP_010818269 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_010824395 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_016622645 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_033624816 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPERLLTLLYHA 1217 WP_033625576 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_033789179 1157 NPI---LFLE---EKGFLR--P-RV--LMK--LPKYTLYE---FPEGRRRLLAS AKEAQKGNQMVLPEHLLTLLYHA 1217 WP_002310644 1159 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1219 WP_002312694 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_002314015 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_002320716 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_002330729 1159 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1219 WP_002335161 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_002345439 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_034867970 1150 DPT---TFLK---EKGFPQ--V-TEF--IK--LPKYTLFE---FDNGRRRFLAS -KESQKGNPFILSDQLVTLLYHA 1210 WP_047937432 1160 SPV---LFLK---NKGYEQ--A-EIE--MK--LPKYALFE---LENGRKRMVAS -KEAQKANSFLLPEHLVTLLYHA 1220 WP_010720994 1150 DPT---TFLK---DKGFPQ--V-TEF--IK--LPKYTLFE---FDNGRRRFLAS -KESQKGNPFILSDQLVTLLYHA 1210 WP_010737004 1150 DPT---TFLK---EKGFPQ--V-TEF--IK--LPKYTLFE---FDNGRRRFLAS -KESQKGNPFILSDQLVTLLYHA 1210 WP_034700478 1150 DPT---TFLK---DKGFPH--V-TEF--IK--LPKYTLFE---FDNGRRRFLAS -KESQKGNPFILSDQLVTLLYHA 1210 WP_007209003 1148 NPIl---YLS---KKDYHN---pKVEAI----LPKYSLFE---FENGRRRMVAS -SETQKGNQLIIPGHLMELLYHS 1208 WP_023519017 1144 DPR---EFLK---TKGYEG--V-KQW--LI--LPKYILFE---AQGGYRRMIAS -QETQKANSLILPENLVTLLYHA 1204 WP_010770040 1155 DPV---SLLE---EKGYAN--P-EV--LIH--LPKYTLYE---LENGRRRLLAS ANEAQKGNQLVLPASLVTLLYHA 1215 WP_048604708 1152 NER---EFLK---NKGYQN--P-QI--CMK--LPKYSLYE---FDDGRRRLLAS AKEAQKGNQMVLPAHLVTFLYHA 1212 WP_010750235 1153 DPI---SFLI---EKGYSN--V-NQF--IK--LPKYTLFE---LANGQRRMLAS -QELQKANSFILPEKLVTLLYHA 1213 AII16583 1216 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1278 WP_029073316 1165 TKI---NYIK--eSEGLEE--VkIIK---E--ILKNQLIEi----NGGLFYVTS --EIVNARQLILDFNCTRIIDGI 1225 WP_031589969 1165 IKI---NYLK--qAEDLEE--VqIGK---E--ILKNQLIEk----DGGLYYIVA --EIINAKQLILNESQTKLVCEI 1225 KDA45870 1139 DPT---AYLA---SRGYTNvtNsFIL-------PKYSLLEd---PEGRRRYLAS -KEFQKANELILPQHLVELLYWV 1199 WP_039099354 1171 QKI-spQFTKv---KKQKGtiV-KVVEDFEv-IAPHILINqrfFDNGQELTLGS ----HNEQELILDKTAVKLLNGA 1241 AKP02966 1173 KTL--qNWLE---ENVKHKksIqIIK---Nn-VPIGQIIY------SKKVGLLS -REIANRQQLILPPEHSALLRIL 1237 WP_010991369 1155 DEK---AFLE---EQGYRQ--P-KV--LAK--LPKYTLYE---CEEGRRRMLAS ANEAQKGNQQVLPNHLVTLLHHA 1215 WP_033838504 1155 DEK---AFLE---EQGYRQ--P-KV--LAK--LPKYTLYE---CEEGRRRMLAS ANEAQKGNQQVLPNHLVTLLHHV 1215 EHN60060 1158 DEK---AFLE---EQGYRQ--P-KV--LAK--LPKYTLYE---CEEGRRRMLAS ANEAQKGNQQVLPNHLVTLLHHV 1218 EFR89594 924 DEK---AFLE---EQGYRQ--P-KV--LAK--LPKYTLYE---CEEGRRRMLAS ANEAQKGNQQVLPNHLVTLLHHA 984 WP_038409211 1155 DQK---AFLE---EKGYYS--P-KV--LTK--IPKYTLYE---CENGRRRMLGS ANEAQKGNQMVLPNHLMTLLYHA 1215 EFR95520 774 DQK---AFLE---EKGYYS--P-KV--LTK--IPKYTLYE---CENGRRRMLGS ANEAQKGNQMVLPNHLMTLLYHA 834 WP_003723650 1155 DEE---AFLE---EKGYRH--P-KV--LTK--LPKYTLYE---CEKGRRRMLAS ANEAQKGNQLVLSNHLVSLLYHA 1215 WP_003727705 1155 DEE---AFLE---EKGYHQ--P-KV--LTK--LPKYTLYE---CEKGRRRMLSS ANEAQKGNQLVLSNHLVSLLYHA 1215 WP_003730785 1155 DEE---AFLE---EKGYHQ--P-KV--LTK--LPKYTLYE---CEKGRRRMLSS ANEAQKGNQLVLSNHLVSLLYHA 1215 WP_003733029 1155 DEK---TFLE---EKGYHQ--P-KV--LIK--VPKYTLYE---CKNGRRRMLGS ANEAHKGNQMLLPNHLMALLYHA 1215 WP_003739838 1155 DEK---SFLE---KQGYRQ--P-KV--LTK--LPKYTLYE---CENGRRRMLAS ANEAQKGNQQVLKGQLITLLHHA 1215 WP_014601172 1155 DEE---AFLE---EKGYRH--P-KV--LTK--LPKYTLYE---CEKGRRRMLAS ANEAQKGNQLVLSNHLVSLLYHA 1215 WP_023548323 1155 DEK---VFLE---GKGYHQ--P-KV--LTK--LPKYALYE---CENGRRRMLGS ANEVHKGNQMLLPNHLMTLLYHA 1215 WP_031665337 1155 DEE---AFLE---EKGYRH--P-KV--LTK--LPKYTLYE---CEKGRRRMLAS ANEAQKGNQLVLSNHLVSLLYHA 1215 WP_031669209 1155 DEK---TFLE---EKGYHQ--P-KV--LIK--VPKYTLYE---CENGRRRMLGS ANEAHKGNQMLLPNHLMALLYHA 1215 WP_033920898 1155 DEK---VFLE---GKGYHQ--P-KV--LTK--LPKYALYE---CENGRRRMLGS ANEVHKGNQMLLPNHLMTLLYHA 1215 AKI42028 1158 DEE---AFLE---EKGYRH--P-KV--LTK--LPKYTLYE---CEKGRRRMLAS ANEAQKGNQLVLSNHLVSLLYHA 1218 AKI 50529 1158 DEK---VFLE---GKGYHQ--P-KV--LTK--LPKYALYE---CENGRRRMLGS ANEVHKGNQMLLPNHLMTLLYHA 1218 EFR83390 603 DEE---AFLE---EKGYRH--P-KV--LTK--LPKYTLYE---CEKGRRRMLAS ANEAQKGNQLVLSNHLVSLLYHA 663 WP_046323366 1155 DQK---EFLE---GKGYRN--P-KV--ITK--IPKYTLYE---CENGRRRMLGS ANEAQKGNQMVLPNHLMTLLYHA 1215 AKE81011 1193 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1255 CUO82355 1154 EQI---EYVE--kEEKLSD--VkIIK---Nn-IPLNQLIEi----DGRQYLLTS --ECVNAMQLVLNEEQCKLIADI 1215 WP_033162887 1155 EQL---SYIAspeHEDLID--VrIVK---E--ILKNQLIEi----DGGLYYVTS --EYVTARQLSLNEQSCKLISEI 1217 AGZ01981 1210 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1272 AKA60242 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 AKS40380 1177 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1239 4UN5_B 1181 NPI---DFLE---AKGYKE--V-KKDLIIK--LPKYSLFE---LENGRKRMLAS -GELQKGNELALPSKYVNFLYLA 1243 WP_010922251 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_039695303 1249 HRAD----NFNS-TEYLN--YVSEHKKEFEKVLSCVEDFANLYVDVE--KNLSKIR-A VAD-SM---DNFSIEE-- 1308 WP_045635197 1247 KNVH----KLDE-PGHLE--YIQKHRNEFKDLLNLVSEFSQKYVLAD--ANLEKIK-S LYA-DN---EQADIEI-- 1306 5AXW_A 979 GELYRVIgVNND1LNRIE---VNMIDITYREYLENMNDKRPPRIIKTiaSKTQSIK-K LYEyKSk--KHPQIIKkg 1056 WP_009880683 924 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 989 WP_010922251 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_011054416 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_011284745 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_011285506 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_011527619 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_012560673 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_014407541 1239 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1304 WP_020905136 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_023080005 1239 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1304 WP_023610282 1239 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1304 WP_030125963 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_030126706 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_031488318 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_032460140 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_032461047 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_032462016 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_032462936 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_032464890 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_033888930 1065 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1130 WP_038431314 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_038432938 1239 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1304 WP_038434062 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 BAQ51233 1151 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1216 KGE60162 415 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 480 KGE60856 178 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 243 WP_002989955 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 WP_003030002 1222 YQIE----KNYE-PEHRE--YVEKHKDEFKELLEYISVFSRKYVLAD--NNLTKIE-M LFS-KN---KDAEVSS-- 1281 WP_003065552 1250 QRIN----SFNS-TKYLD--YVSAHKKEFEKVLSCVEDFANLYVDVE--KNLSKIR-A VAD-SM---DNFSIEE-- 1309 WP_001040076 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDIFQIINDFSKRVILAD--ANLEKIN-R LYQ-DNk--ENIPVDE-- 1306 WP_001040078 1249 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1314 WP_001040080 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040081 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040083 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040085 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040087 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040088 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040089 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040090 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040091 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040092 1241 SRYNESKgKPEEiEKKQE--FVNQHISYFDDILQLINDFSKRVILAD--ANLEKIN-K LYS-DNk--DNTPVDE-- 1306 WP_001040094 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040095 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040096 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040097 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040098 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040099 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040100 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_001040104 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040105 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040106 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040107 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040108 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040109 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_001040110 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_015058523 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYS-DNk--DNTPVDE-- 1306 WP_017643650 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_017647151 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_017648376 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_017649527 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_017771611 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_017771984 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 CFQ25032 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 CFV16040 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 KLJ37842 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 KLJ72361 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 KLL20707 1255 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1320 KLL42645 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_047207273 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_047209694 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENIPVDE-- 1306 WP_050198062 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_050201642 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_050204027 1241 SRYNELKgKPEEiEQKQE--FVVQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_050881965 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_050886065 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 AHN30376 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYS-DNk--DNTPVDE-- 1306 EAO78426 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQLINDFSKRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 CCW42055 1241 SRYNESKgKPEEiEKKQE--FVNQHVSYFDDILQIINDFSNRVILAD--ANLEKIN-K LYQ-DNk--ENISVDE-- 1306 WP_003041502 1254 KRIN----NPIN-KDHIE--YVKKHRDDFKELLNYVLEFNEKYVGAT--KNGERLK-E AVA-DF---DSKSNEE-- 1313 WP_037593752 1223 YQIE----KNYE-PEHRE--YVEKHKDEFKELLEYISVFSRKYVLAD--NNLTKIE-M LFS-KN---KDAEVSS-- 1282 WP_049516684 1223 YRIE----KDYE-PEHRE--YVEKHKDEFKELLEYISVFSRKYVLAD--NNLTKIE-M LFS-KN---KDAEVSS-- 1282 GAD46167 1222 YQIE----KNYE-PEHRE--YVEKHKDEFKELLEYISVFSRKYVLAD--NNLTKIE-M LFS-KN---KDAEVSS-- 1281 WP_018363470 1254 HRIG----NFNS-AEHLK--YVSEHKKEFEEVLSCVENFANVYVDVE--KNLSKIR-A AAD-SM---DNFSIEE-- 1313 WP_003043819 1249 QNISATIgSNNLg-------YIEQHREEFKEIFEKIIDFSEKYILKN--KVNSNLK-S SFD-EQfavSDSIL--l- 1310 WP_006269658 1222 YRIE----KDYE-PEHRE--YVEKHKDEFKELLEYISVFSRKYVLAD--NNLTKIE-M LFS-KN---KDAEVSS-- 1281 WP_048800889 1242 HRID----NSDN-SEHLK--YITEHKEEFGKLLSYIENFAKSYVDVD--KNLEKIQ-L AVE-KI---DSFSVKE-- 1301 WP_012767106 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_014612333 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_015017095 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_015057649 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_048327215 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_049519324 1246 -HAHKIEsSKE--LEHEA--YILDHYNDLYQLLSYIERFASLYVDVE--KNISKVK-E LFS-NI---ESYSISEi- 1308 WP_012515931 1220 AHYHELIgSSEDvLRKKY--FVDRHLHYFDDIIQMINDFAERHILAS--SNLEKIN-H TYH-NN---SDLPVNEr- 1285 WP_021320964 1220 AHYHELIgSSEDvLRKKY--FVERHLHYFDDIIQMINDFAERHILAS--SNLEKIN-H TYH-NN---SDLPINEr- 1285 WP_037581760 1220 AHYHELIgSSEDvLRKKY--FVERHLHYFDDIIQMINDFAERHILAS--SNLEKIN-H TYH-NN---SDLPVNEr- 1285 WP_004232481 1252 QHVN----NSHK-PEHLN--YVKQHKDEFKDIFNLIISIARINILKP--KVVDNL--- -IN-EF---TEYGQED-- 1308 WP_009854540 1247 HRAD----NFNS-TEYLN--YVSEHKKEFEKVLSCVEDFANLYVDVE--KNLSKIR-A VAD-SM---DNFSIEE-- 1306 WP_012962174 1248 HRVN----SFNN-SEHLK--YVSEHKKEFGEVLSCVENFAKSYVDVE--KNLGKIR-A VAD-KI---DTFSIED-- 1307 WP_039695303 1249 HRAD----NFNS-TEYLN--YVSEHKKEFEKVLSCVEDFANLYVDVE--KNLSKIR-A VAD-SM---DNFSIEE-- 1308 WP_014334983 1252 HRID----SFNS-TEHLK--YVSEHKKEFEKVLSCVENFSNLYVDVE--KNLSKVR-A AAE-SM---TNFSLEE-- 1311 WP_003099269 1240 SHYTKFIgKEEDrEKKRS--YVESHLYYFDEIMQIIVEYSNRYILAD--SNLIKIQ-N LYK-EKd---NFSIEEq- 1305 AHY15608 1240 SHYTKFTgKEEDrEKKRS--YVESHLYYFXEVKSSF---------------------- ------------------ 1273 AHY17476 1240 SHYTKFTgKEEDrEKKRS--YVESHLYXFX---------------------------- ------------------ 1267 ESR09100 72 SHYTKFIgKEEDrEKKRS--YVESHLYYFDEIMQIIVEYSNRYILAD--SNLIKIQ-N LYK--Ek--DNFSIEEq- 137 AGM98575 1240 SHYTKFTgKEEDrEKKRS--YVESHLYYFDVRLSQVFRVTNVEF-------------- ------------------ 1281 ALF27331 1222 KNIH----KVDE-PKHLD--YVKKHKDEFKELLDVVSNFSKKNILAE--SNLEKIE-E LYA-QN---NNKDITE-- 1281 WP_018372492 1229 KKVDVLVkSKDD---DYD---LEEHRAEFAELLDCIKKFNDMYILAS--SNMSKIE-E IYQ-KNi---DAPIEE-- 1289 WP_045618028 1252 QRIN----KISE-PIHKQ--YVETHQSEFKELLTAIISLSKKYI-QK--PNVESL--- LQQ-AF---DQSDKDIyq 1310 WP_045635197 1247 KNVH----KLDE-PGHLE--YIQKHRNEFKDLLNLVSEFSQKYVLAD--ANLEKIK-S LYA-DN---EQADIEI-- 1306 WP_002263549 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002263887 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002264920 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002269043 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002269448 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002271977 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002272766 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002273241 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002275430 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002276448 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002277050 1230 HHL-------DN-DYSNE--YVKNHYQQFDILFNEITSFSKKCKLGK--EHIQKIE-E AYSkER---DSASIEE-- 1287 WP_002277364 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002279025 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002279859 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002280230 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002281696 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002282247 1230 HHL-------DN-DYSNE--YVKNHYQQFDILFNEITSFSKKCKLGK--EHIQKIE-E AYSkER---DFASIEE-- 1287 WP_002282906 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002283846 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002287255 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002288990 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002289641 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002290427 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002295753 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002296423 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002304487 1236 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1295 WP_002305844 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002307203 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002310390 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_002352408 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_012997688 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_014677909 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019312892 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019313659 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019314093 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019315370 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019803776 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_019805234 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_024783594 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_024784288 1230 HHL-------DN-DYSNE--YVKNHYQQFDILFNEITSFSKKCKLGK--EHIQKIE-E AYSkER---DFASIEE-- 1287 WP_024784666 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_024784894 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_024786433 1230 HHL-------DN-DYSNE--YVKNHYQQFDILFNEITSFSKKCKLGK--EHIQKIE-E AYSkER---DSASIEE-- 1287 WP_049473442 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 WP_049474547 1222 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1281 EMC03581 1215 KNIH----KVDE-PKHLD--YVDKHKDEFKELLDVVSNFSKKYTLAE--GNLEKIK-E LYA-QN---NGEDLKE-- 1274 WP_000428612 1250 KNIH----RLDE-PEHLE--YIQKHRNEFKGLLNLVSEFSQKYVLAD--ANLEKIK-N LYA-DN---EQADIEI-- 1309 WP_000428613 1248 KNVH----KLDE-PEHLE--YIQKHRNEFKDLLNLVSEFSQKYVLAD--ANLEKIQ-N LYA-DN---EQADIEI-- 1307 WP_049523028 1243 KRIQ----KKDE-PEHLE--YIKQHHSEFNDLLNFVSEFSQKYVLAE--SNLEKIK-N LYI-DN---EQTNMEE-- 1302 WP_003107102 1209 SRYTSFSgKEEDrEKHRH--FVESHLHYFDEIKDIIADFSRRYILAD--ANLEKIL-T LYN-EKn---QFSIEEg- 1274 WP_054279288 1242 SKRDK--gTQSEnME-----YISNHKEKFIEIFHYIIRYAEKNVIKP--KVIERLN-D TFNqKF---NDSDLTEl- 1303 WP_049531101 1252 QRIN----KISE-PIHKQ--YVETHQSEFEELLTTIISLSKKYI-QK--PIVESL--- LQQ-AF---EQADKDIyg 1310 WP_049538452 1252 QRIN----KISE-PIHKQ--YVEAHQNEFKELLTTIISLSKKYI-QK--PNVESL--- LQQ-AF---EQADKDIyg 1310 WP_049549711 1254 QRIN----KFSE-PIHKQ--YVEAHQNEFKELLTIIISLSKKYI-QK--PNVESL--- LHQ-AF---EQADNDIyg 1312 WP_007896501 1246 SRYDKLSsKIESeQQKKL--FVEQHLHYFDEILDIVVKHATCYIKAE--NNLKKII-S LYK-KK---EAYSINEg- 1311 EFR44625 1198 SRYDKLSsKIESeQQKKL--FVEQHLHYFDEILDIVVKHATCYIKAE--NNLKKII-S LYK-KK---EAYSINEg- 1263 WP_002897477 1247 KNLH----KLDE-PEHLE--YIQKHRNEFKDLLNLVSEFSQKYILAE--ANLEKIK-D LYA-DN---EQADIEI-- 1306 WP_002906454 1253 QRIN----KISE-PIHKQ--YVEAHQNEFKELLTTIISLSKKYI-QK--PNVELL--- LQQ-AF---DQADKDIyg 1311 WP_009729476 1248 KNVH----KLDE-PGHLE--YIQKHRNEFKDLLNLVSEFSQKYVLAD--ANLEKIK-N LYA-DN---EQADIEI-- 1307 CQR24647 1238 QHAN----KEDS----VI--YLEKHRHELSELFHHIIGVSEKTILKP--KVEMTLN-E AFE-KHf--EFDEVSE-- 1295 WP_000066813 1252 KNVH----KLDE-PEHLE--YIQKHRYEFKDLLNLVSEFSQKYVLAD--ANLEKIK-N LYA-DN---EQADIEI-- 1311 WP_009754323 1248 KNVH----KLDE-PEHLE--YIQKHRYEFKDLLNLVSEFSQKYVLAE--ANLEKIK-S LYV-DN---EQADIEI-- 1307 WP_044674937 1241 SNIH----KITE-PIHLN--YVNKNKHEFKELLRHISDFSTRYILAQ--DRLSKIE-E LYD-KN---DGDDISD-- 1300 WP_044676715 1243 SNIH----KITE-PIHLN--YVNKNKHEFKELLRHISDFSTRYILAQ--DRLSKIE-E LYD-KN---DGDDISD-- 1302 WP_044680361 1243 SNIH----KITE-PIHLN--YVNKNKHEFKELLRHISDFSTRYILAQ--DRLSKIE-E LYD-KN---DGDDISD-- 1302 WP_044681799 1241 SNIH----KITE-PIHLN--YVNKNKHEFKELLRHISDFSTRYILAQ--DRLSKIE-E LYD-KN---DGDDISD-- 1300 WP_049533112 1254 KRIN----NPIN-KDHIE--YVKKHRDDFKELLNYVLEFNEKYVGAT--KNGERLK-E AVA-DF---DSKSNEE-- 1313 WP_029090905 1182 STKQA-----DE-AMFLKyyRLEHLEAVFEEL---IRKQAADYQIFE--KLIKKIEvN FYS----c----TYNEk- 1240 WP_006506696 1212 YNAIYKQ-DYDNlDDILMi-----------QLYIELTNKMKVLYPAY-rGIAEKFE-S YVV----i----SKEEk- 1268 AIT42264 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREg- 1305 WP_034440723 1218 KHYNE-----DE--TSHK--FIVEHKAYFDELLNYIVEFANKYLELE--NSIEKIK-D LYH-----gKGPDVEEke 1276 AKQ21048 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREg- 1305 WP_004636532 1212 NRYDKVK-----fPDSIE--YVHDNLAKFDDLLEYVIDFSNKYINAD--KNVQKIQ-K IYK-EH---GTEDVEL-- 1271 WP_002364836 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_016631044 1169 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1228 EMS75795 954 QHYDEIAhKESF-----D--YVNDHLSEFREILDQVIDFSNRYTIAA--KNTEKIA-E LFE-QN---QESTVQS-- 1013 WP_002373311 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_002378009 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_002407324 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_002413717 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_010775580 1220 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1279 WP_010818269 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_010824395 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_016622645 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_033624816 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_033625576 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-TN---QTADVKE-- 1277 WP_033789179 1218 KQCLL----PNQ-SESLA--YVEQHQPEFQEILERVVDFAEVHTLAK--SKVQQIV-K LFE-AN---QTADVKE-- 1277 WP_002310644 1220 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1279 WP_002312694 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_002314015 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_002320716 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_002330729 1220 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1279 WP_002335161 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_002345439 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_034867970 1211 QHYDKITyQESF-----D--YVNTHLSDFSAILTEVLAFAEKYTLAD--KNIERIQ-E LYE-EN---KYGETSM-- 1270 WP_047937432 1221 KQYDEIShKESF-----D--YVNEHHKEFSEVFARVLEFAGKYTLAE--KNIEKLE-K IYK-EN---QTDDLAK-- 1280 WP_010720994 1211 QHYDKITyQESF-----D--YVNTHLSDFSAILTEVLAFAEKYTLAD--KNIERIQ-E LYE-EN---KYGEISM-- 1270 WP_010737004 1211 QHYDKITyQESF-----D--YVNTHLSDFSAILTEVLAFAEKYTLAD--KNIERIQ-E LYE-EN---KYGETSM-- 1270 WP_034700478 1211 QHYDKITyQESF-----D--YVNTHLSDFSAILTEVLAFAEKYTLAD--KNIERIQ-E LYE-EN---KYGEISM-- 1270 WP_007209003 1209 KKIIN--gKNSD---SVS--YIQNNKEKFREIFEYIVDFSSKYISAD--ANLNKIE-K IFE-NNfh----KASEge 1269 WP_023519017 1205 RHYDEINhKVSF-----D--YVNAHKEGFNDIFDFISDFGVRYILAP--QHLEKIK-V AYE-KN---KEVDLKE-- 1264 WP_010770040 1216 KQVDE-----DS-GKSEE--YVREHRAEFAEILNYVQAFSETKILAN--KNLQTIL-K LYE-EN---KEADIKE-- 1274 WP_048604708 1213 KHCNE-----KP-D-SLK--YVTEHQSGFSEIMAHVKDFAEKYTLVD--KNLEKIL-S LYA-KN---MDSEVKE-- 1270 WP_010750235 1214 NHYDEIAyKDSY-----D--YVNEHFSNFQDILDKVIIFAEKYTSAP--QKLNQII-A TYE-KN---QEADRKI-- 1273 AII16583 1279 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREg- 1344 WP_029073316 1226 YKAMKYK-NYSElSQEEIm-----------NVYDIFVEKLKLYYPTY-kNIAINFE-N FEN----i----SDEEk- 1282 WP_031589969 1226 YKAMKYK-NYDNlDSEKIi-----------DLYRLLINKMELYYPEYrkQLVKKFE-D LKV----i----SIEEk- 1283 KDA45870 1200 NAKDG--------EQKLE-----DHKAEFKELFDKIMEFADKYVVAP--KNSEKIR-R LYE-ENg-----DATPme 1253 WP_039099354 1242 LPLTQ-----SEeLAEQV----------YDEILDQVMHYFPLYDINQfrAKLSAGKaA DGN-KMv-----QVGQgv 1306 AKP02966 1238 QIPDE------DpDQILAf----YDKNILVEILQELITKMKKFYPFY--KNEQEFLaS FNQ--------ATTSEk- 1296 WP_010991369 1216 ANCEV-----SD-GKSLD--YIESNREMFAELLAHVSEFAKRYTLAE--ANLNKIN-Q LFE-QN---KEGDIKA-- 1274 WP_033838504 1216 ANCEV-----SD-GKSLD--YIESNREMFAELLAHVSEFAKRYTLAE--ANLNKIN-Q LFE-QN---KEGDIKA-- 1274 EHN60060 1219 ANCEV-----SD-GKSLD--YIESNREMFAELLAHVSEFAKRYTLAE--ANLNKIN-Q LFE-QN---KEGDIKA-- 1277 EFR89594 985 ANCEV-----SD-GKSLD--YIESNREMFAELLAHVSEFAKRYTLAE--ANLNKIN-Q LFE-QN---KEGDIKA-- 1043 WP_038409211 1216 KNCEA-----ND-GESLA--YIEMHREMFAELLAYISEFAKRYTLAN--DRLEKIN-M FFE-QN---KKGDIKV-- 1274 EFR95520 835 KNCEA-----ND-GESLA--YIEMHREMFAELLAYISEFAKRYTLAN--DRLEKIN-M FFE-QN---KKGDIKV-- 893 WP_003723650 1216 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATRYTLAD--ANLSKIN-N LFE-QN---KEGDIKA-- 1274 WP_003727705 1216 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATKYTLAD--ANLSKIN-N LFE-QN---KEGDIKA-- 1274 WP_003730785 1216 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATKYTLAD--ANLSKIN-N LFE-QN---KEGDIKA-- 1274 WP_003733029 1216 EKYEA-----ID-GESLA--YIEVHRALFDELLAYISEFARKYTLSN--DRLDEIN-M LYE-RN---KDGDVKS-- 1274 WP_003739838 1216 KNCEA-----SD-GKSLD--YIESNREMFGELLAHVSEFAKRYTLAD--ANLSKIN-Q LFE-QN---KDNDIKV-- 1274 WP_014601172 1216 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATRYTLAD--ANLSKIN-N LFE-QN---KEGDIQA-- 1274 WP_023548323 1216 EKREA-----ID-GESLA--YIEAHKAVFGELLAHISEFARKYTLAN--DKLDEIN-M LYE-RN---KDGDVKS-- 1274 WP_031665337 1216 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATRYTLAD--ANLSKIN-N LFE-QN---KEGDIKA-- 1274 WP_031669209 1216 EKYEA-----ID-GESLA--YIEVHRALFDELLAYISEFARKYTLSN--DRLDEIN-M LYE-RN---KDGDVKS-- 1274 WP_033920898 1216 EKREA-----ID-GESLA--YIEAHKAVFGELLAHISEFARKYTLAN--DKLDEIN-M LYE-RN---KDGDVKS-- 1274 AKI42028 1219 KNCEA-----SD-GKSLK--YIEAHRETFSELLAQVSEFATRYTLAD--ANLSKIN-N LFE-QN---KEGDIQA-- 1277 AKI50529 1219 EKREA-----ID-GESLA--YIEAHKAVFGELLAHISEFARKYTLAN--DKLDEIN-M LYE-RN---KDGDVKS-- 1277 EFR83390 664 KNCEA-----SD-GKSLK--YTEAHRETFSELLAQVSEFATRYTLAD--ANLSKIN-N LFE-QN---KEGDIKX-- 722 WP_046323366 1216 KNCEA-----SD-GKSLA--YIESHREMFAELLDSISEFASRYTLAD--ANLEKIN-T IFE-QN---KSGDVKV-- 1274 AKE81011 1256 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1321 CUO82355 1216 YNAIYKQ-DEDGlDNMLMi-----------QLYLQLIDKLKTLYPIY-mGIVEKFE-K FVS----i----SKEEk- 1272 WP_033162887 1218 YAAMLKK-RYEYlDEEEIf-----------DLYLQLLQKMDTLYPAY-kGIAKRFF-D FKN----i----DVVEk- 1274 AGZ01981 1273 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1338 AKA60242 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 AKS40380 1240 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1305 4UN5_B 1244 SHYEKLKgSPEDnEQKQL--FVEQHKHYLDEIIEQISEFSKRVILAD--ANLDKVL-S AYN-KH---RDKPIREq- 1309 WP_010922251 1306

1365 WP_039695303 1309 ISN---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL NATLIHQSITGLYETRIDLSKL-- 1369 WP_045635197 1307 LAN---SFI NLLTFTALGAP-AAFKFFG--KDI--DRK--R-YTTVSEIL NATLIHQSITGLYETWIDLSKL-- 1367 5AXW_A --------- ----------------------------------------- ------------------------ WP_009880683 990 -AE---NII HLFTLTNLGAP-AAFKCFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1049 WP_010922251 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_011054416 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_011284745 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATFIHQSITGLYETRIDLSQL-- 1365 WP_011285506 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_011527619 1306 -AE---NII HLFTLTNLGAP-TAFKYFD--TTI--DRK--R-YTSTKEVL DATFIHQSITGLYETRIDLSQL-- 1365 WP_012560673 1306 -AE---NII HLFTLTNLGAP-AAFKCFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_014407541 1305 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1364 WP_020905136 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_023080005 1305 -AK---NII HLFTLTNLGAP-AAFKYFD--TTI--ERN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1364 WP_023610282 1305 -AK---NII HLFTLTNLGAP-AAFKYFD--TTI--ERN--R-YKSIKEVL DATLIHQSITGLYEIRIDLSQL-- 1364 WP_030125963 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_030126706 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_031488318 1306 -AE---NII HLFTLTNFGAP-AAFIYFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_032460140 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_032461047 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_032462016 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_032462936 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_032464890 1306 -AE---NII HLFTLTNLGAP-TAFKYFD--TTI--DRK--R-YTSTKEVL DATFIHQSITGLYETRIDLSQL-- 1365 WP_033888930 1131 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1190 WP_038431314 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_038432938 1305 -AK---NII HLFTLTNLGAP-AAFKYFD--TTI--ERN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1364 WP_038434062 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--GRN--R-YKSIKEVL DATLIHQSITGLYETRIDLSQL-- 1365 BAQ51233 1217 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1276 KGE60162 481 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 540 KGE60856 244 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 303 WP_002989955 1306 -AE---NII HLFTLTNLGAP-TAFKYFD--TTI--DRK--R-YTSTKEVL DATFIHQSITGLYETRIDLSQL-- 1365 WP_003030002 1282 LAK---SFI SLLTFTAFGAP-AAFNFFG--ENI--DRK--R-YTSVTECL NATLIHQSITGLYETRIDLSKL-- 1342 WP_003065552 1310 ISN---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL NATLIHQSITGLYETRIDLSKI-- 1370 WP_001040076 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040078 1315 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLSKL-- 1375 WP_001040080 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040081 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040083 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040085 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040087 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040088 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040089 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040090 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040091 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040092 1307 LAK---NII NLFTFTSLGAP-AAFKFFD--KSV--DRK--R-YTSTKEVL DSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040094 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040095 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040096 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040097 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040098 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040099 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHKSITGLYETRIDLGKL-- 1367 WP_001040100 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040104 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040105 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040106 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040107 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040108 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_001040109 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQFITGLYETRIDLGKL-- 1367 WP_001040110 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_015058523 1307 LAK---NII NLFTFTSLGAP-AAFKFFD--KSV--DRK--R-YTSTKEVL DSTLIHQSITGLYETRIDLGKL-- 1367 WP_017643650 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_017647151 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_017648376 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_017649527 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_017771611 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_017771984 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 CFQ25032 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 CFV16040 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 KLJ37842 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 KLJ72361 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 KLL20707 1321 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1381 KLL42645 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_047207273 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_047209694 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHKSITGLYETRIDLGKL-- 1367 WP_050198062 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_050201642 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_050204027 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_050881965 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KII--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_050886065 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 AHN30376 1307 LAK---NII NLFTFTSLGAP-AAFKFFD--KSV--DRK--R-YTSTKEVL DSTLIHQSITGLYETRIDLGKL-- 1367 EAO78426 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 CCW42055 1307 LAN---NII NLFTFTSLGAP-AAFKFFD--KIV--DRK--R-YTSTKEVL NSTLIHQSITGLYETRIDLGKL-- 1367 WP_003041502 1314 ICT---SFL GLFELTSLGSA-SDFEFLG--VKI--PRY--RdYTPSSLLK DSTLIHQSITGLYETRIDLSKL-- 1383 WP_037593752 1283 LAK---SFI SLLTFTAFGAP-AAFNFFG--ENI--DRK--R-YTSVTECL NATLIHQSITGLYETRIDLSKL-- 1343 WP_049516684 1283 LAK---SFI SLLTFTAFGAP-AAFNFFG--ENI--DRK--R-YTSVTECL NATLIHQSITGLYETRIDLSKL-- 1343 GAD46167 1282 LAK---SFI SLLTFTAFGAP-AAFNFFG--ENI--DRK--R-YTSVTECL NATLIHQSITGLYETRIDLSKL-- 1342 WP_018363470 1314 ISD---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YNSTKECL NATLIHQSITGLYETRIDLSKL-- 1374 WP_003043819 1311 -SN---SFV SLLKYTSFGAS-GGFTFLD--LDVkgGRL--R-YQTVTEVL DATLIYQSITGLYETRTDLSQL-- 1372 WP_006269658 1282 LAK---SFI SLLTFTAFGAP-AAFNFFG--ENI--DRK--R-YTSVTECL NATLIHQSITGLYETRIDLSKL-- 1342 WP_048800889 1302 ISN---SFI HLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL NATLIHQSITGLYETQTDLSKL-- 1362 WP_012767106 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_014612333 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_015017095 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_015057649 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_048327215 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_049519324 1309 -CS---SVI NLLTLTASGAP-ADFKFLG--TTI--PRK--R-YGSPQSIL SSTLIHQSITGLYETRIDLSQL-- 1368 WP_012515931 1286 -AE---NII NVFTFVALGAP-AAFKFFD--ATI--DRK--R-YTSTKEVL NATLIHQSVTGLYETRIDLSQL-- 1345 WP_021320964 1286 -AE---NII NVFTFVALGAP-AAFKFFD--ATI--DRK--R-YTSTKEVL NATLIHQSVTGLYETRIDLSQL-- 1345 WP_037581760 1286 -AE---NII NVFTFVALGAP-AAFKFFD--ATI--DRK--R-YTSTKEVL NATLIHQSVTGLYETRIDLSQL-- 1345 WP_004232481 1309 ISSlseSFI NLLKFISFGAP-GAFKFLK--LDV--KQSnlR-YKSTTEAL SATLIHQSVTGLYETRIDLSKL-- 1374 WP_009854540 1307 ISN---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL TATLIHQSITGLYETRIDLSKL-- 1367 WP_012962174 1308 ISI---SFV NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL NATLIHQSITGLYETRIDLSKL-- 1368 WP_039695303 1309 ISN---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL NATLIHQSITGLYETRIDLSKL-- 1369 WP_014334983 1312 ISA---SFI NLLTLTALGAP-ADFNFLG--EKI--PRK--R-YTSTKECL SATLIHQSVTGLYETRIDLSKL-- 1372 WP_003099269 1306 -AI---NML NLFTFTDLGAP-SAFKFFN--GDI--DRK--R-YSSTNEII NSTLIYQSPTGLYETRIDLSKL-- 1365 AHY15608 --------- ----------------------------------------- ------------------------ AHY17476 --------- ----------------------------------------- ------------------------ ESR09100 138 -AI---NML NLFTFTDLGAP-SAFKFFNg--DI--DRK--R-YSSTNEII NSTLIYQSPTGLYETRIDLSKL-- 197 AGM98575 --------- ----------------------------------------- ------------------------ ALF27331 1282 LAS---SFI NLLTFTAIGAP-AAFKFFD--NNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSRL-- 1342 WP_018372492 1290 VAR---SFV -LLNFTMMGAA-TDFKFFG--QII--PRK--R-YPSTTECL KSTLIHQSVTGLYETRIDLSKL-- 1350 WP_045618028 1311 LSE---SFI SLLKLISFGAP-GTFKFLG--VEI--SQSnvR-YQSVSSCF NATLIHQSITGLYETRIDLSKL-- 1373 WP_045635197 1307 LAN---SFI NLLTFTALGAP-AAFKFFG--KDI--DRK--R-YTTVSEIL NATLIHQSITGLYETWIDLSKL-- 1367 WP_002263549 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_002263887 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_002264920 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002269043 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_002269448 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002271977 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002272766 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002273241 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002275430 1282 LSS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002276448 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002277050 1288 LAD---GFI KLLGFTQLGAT-SPFSFLG--IKL--NQK--Q-YTGKKDYL EATLIHQSITGLYETRIDLNKL-- 1352 WP_002277364 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002279025 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002279859 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002280230 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002281696 1282 LSS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002282247 1288 LAD---GFI KLLGFTQLGAT-SPFSFLG--IKL--NQK--Q-YTGKKDYL EATLIHQSITGLYETRIDLSKL-- 1352 WP_002282906 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002283846 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002287255 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002288990 1282 LAS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002289641 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002290427 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002295753 1282 LAS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002296423 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002304487 1296 LAS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1356 WP_002305844 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002307203 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002310390 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_002352408 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_012997688 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_014677909 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_019312892 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_019313659 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_019314093 1282 LAS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_019315370 1282 LSS---SFI NLLTFTAIGAP-AAFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_019803776 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_019805234 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_024783594 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLNKL-- 1342 WP_024784288 1288 LAD---GFI KLLGFTQLGAT-SPFSFLG--IKL--NQK--Q-YTGKKDYL EATLIHQSITGLYETRIDLSKL-- 1352 WP_024784666 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_024784894 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_024786433 1288 LAD---GFI KLLGFTQLGAT-SPFSFLG--IKL--NQK--Q-YTGKKDYL EATLIHQSITGLYETRIDLSKL-- 1352 WP_049473442 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1342 WP_049474547 1282 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL KATLIHQSITGLYETRIDLSKL-- 1342 EMC03581 1275 LAS---SFI NLLTFTAIGAP-ATFKFFD--KNI--DRK--R-YTSTTEIL NATLIHQSITGLYETRIDLSKL-- 1335 WP_000428612 1310 LAN---SFI NLLTFTALGAP-AAFKFFG--KDV--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1370 WP_000428613 1308 LAN---SFI NLLTFTALGAP-AAFKFFG--KDI--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1368 WP_049523028 1303 IAN---SFI NLLTFTAFGAP-AVFKFFG--KDI--ERK--R-YSTVTEIL KATLIHQSLTGLYETRIDLSKL-- 1363 WP_003107102 1275 -AT---NML NLFTFTGLGAP-ATLKFFN--VDI--DRK--R-YTSSTEIL NSTLIRQSITGLYETRIDLSKI-- 1334 WP_054279288 1304 -SI---SFL NLFKFTSFGAP-EKFTFLN--SEIkgDDV--R-YRSTKECL NSTLIHQSVTGLYETRIDLSQF-- 1365 WP_049531101 1311 LSE---SFI SLLKLTSFGAP-GAFRFLG--VEI--SQSnvR-YQSVSSCF NATLIHQSITGLYETRIDLSKL-- 1373 WP_049538452 1311 LSE---SFI SLLKLTSFGAP-GAFKFLG--VEI--SQSsvR-YKPNSQFL DATLIHQSITGLYETRIDLSKL-- 1373 WP_049549711 1313 LSE---SFI SLLKLTSFGAP-GAFKFLG--AEI--SQSsvR-YKPNSQFL DTTLIHQSITGLYETRIDLSKL-- 1375 WP_007896501 1312 -AL---NML NLFIFTSLGAP-STFVFFD--ETI--DRK--R-YTTSSDVL NGILIQQSITGLYETRIDLSRF-- 1371 EFR44625 1264 -AL---NML NLFIFTSLGAP-STFVFFD--ETI--DRK--R-YTTSSDVL NGILIQQSITGLYETRIDLSRF-- 1323 WP_002897477 1307 LAN---SFI NLLTFTALGAP-AAFKFFG--KDV--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1367 WP_002906454 1312 LSE---SFI SLLKLTSFGAP-GAFKFLG--VEI--SQSsvR-YKPNSQFL DTTLIHQSITGLYETRIDLSKL-- 1374 WP_009729476 1308 LAN---SFI NLLTFTALGAP-AAFKFFG--KDV--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1368 CQR24647 1296 LAQ---SFI SLLKFTAFGAP-GGFKFLD--ADI--KQSnlR-YQTVTEVL SSTLIHQSVTGLYETRIDLSKL-- 1358 WP_000066813 1312 LAN---SFI NLLTFTALGAP-AAFKFLG--KDV--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1372 WP_009754323 1308 LAN---SFI NLLTFTALGAP-AAFKFFG--KDV--DRK--R-YTTVSEIL NATLIHQSITGLYETRIDLSKL-- 1368 WP_044674937 1301 LTS---SFV NLLTFTAIGAP-AAFKFLG--SVI--DRK--R-YTSIAEIL EATLIHQSVTGLYETRIDLSKL-- 1361 WP_044676715 1303 LTS---SFV NLLTFTAIGAP-AAFKFLG--SVI--DRK--R-YTSIAEIL EATLIHQSVTGLYETRIDLSKL-- 1363 WP_044680361 1303 LTS---SFV NLLTFTAIGAP-AAFKFLG--SVI--DRK--R-YTSIAEIL EATLIHQSVTGLYETRIDLSKL-- 1363 WP_044681799 1301 LTS---SFV NLLTFTAIGAP-AAFKFLG--SVI--DRK--R-YTSIAEIL EATLIHQSVTGLYETRIDLSKL-- 1361 WP_049533112 1314 ICT---SFL GLFELTSLGSA-SDFEFLG--VKI--PRY--RdYTPSSLLK DSTLIHQSITGLYETRIDLSKL-- 1383 WP_029090905 1241 -VK----VI ELLKITQANATnGDLKLLK----M-sNREg-R-LGSVSVAL DFKIINQSVTGLYQSIEDYNN--- 1300 WP_006506696 1269 -AN----II QMLIVMHRGPQnGNIVYDDf--KI-sDRIg-R-LKTKNHNL NIVFISQSPTGIYTKKYKL----- 1329 AIT42264 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_034440723 1277 LVE---SFI NLLAITKCGPA-ADITFLG--EKI--SRK--R-YRSTNCLW GSEVIFQSPTGLYETRLRLE---- 1335 AKQ21048 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 WP_004636532 1272 TVE---SFV NLMTFTAMGAP-ATFKFYG--ESI--TRS--R-YTSITEFR GSTLIFQSITGLYETRYKL----- 1329 WP_002364836 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_016631044 1229 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSPTGLYETRRKV----- 1286 EMS75795 1014 LSQ---SFI NLMQLNAMGAP-ADFKFFD--VII--PRK--R-YPSLTEIW ESTITYQSITGLRETRTRMATLwd 1076 WP_002373311 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_002378009 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_002407324 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_002413717 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_010775580 1280 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1337 WP_010818269 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_010824395 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_016622645 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_033624816 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_033625576 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_033789179 1278 IAA---SFI QLMQFNAMGAP-STFKFFQ--KDI--ERA--R-YTSIKEIF DATIIYQSTTGLYETRRKV----- 1335 WP_002310644 1280 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1339 WP_002312694 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_002314015 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_002320716 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_002330729 1280 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1339 WP_002335161 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_002345439 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_034867970 1271 IAQ---SFL QLLQFNAIGAP-ADFKFFG--VTI--PRK--R-YTSLTEIW DATIIYQSVTGLYETRIRMGDLwa 1333 WP_047937432 1281 LAS---SFV NLMQFNAMGAP-ADFKFFD--VTI--PRK--R-YTSLTEIW QSTIIHQSITGLYETRIRMGK--- 1340 WP_010720994 1271 IAQ---SFL QLLQFNAIGAP-ADFKFFG--VTI--PRK--R-YTSLTEIW DATIIYQSVTGLYETRIRMGDLwa 1333 WP_010737004 1271 IAQ---SFL QLLQFNAIGAP-ADFKFFG--VTI--PRK--R-YTSLTEIW DATIIYQSVTGLYETRIRMGDLwa 1333 WP_034700478 1271 IAQ---SFL QLLQFNAIGAP-ADFKFFG--VTI--PRK--R-YTSLTEIW DATIIYQSVTGLYETRIRMGDLwa 1333 WP_007209003 1270 IAK---SFI NLLIFTAMGAP-ADFEFFG--EKI--PRK--R-YVSISEII DAVFIHQSITGLYETRVRLTEV-- 1330 WP_023519017 1265 MID---AIL SLLKFTLFGAS-VEFKFFD--IKI---LK--R-YKSLTDIW EATIIYQSVTGLYERRVEVRKLwd 1326 WP_010770040 1275 IAE---SFV NLMKFSAYGAP-MDFKFFG--KTI--PRS--R-YTSVGELL SATIINQSITGLYETRRKL----- 1332 WP_048604708 1271 IAQ---SFV DLMQLNAFGAP-ADFKFFG--ETI--PRK--R-YTSVNELL EATIINQSITGLYETRRRL----- 1328 WP_010750235 1274 MAH---SFV NLMQFNALGAP-ADFKFFD--TTI--TRK--R-YISLTEIW QSTIIYQSVTGLYETRRRMADLwd 1336 AII16583 1345 -AE---NII HLFTLINLGAP-AAFKYFD--TTI--DRK--R-YISTKEVL DATLIHQSITGLYETRIDLSQL-- 1404 WP_029073316 1283 -CE----VI QMLVVMHAGPQnGNITFDDf--KL-sNRLg-R-LNCKTISL TIVFIADSPIGMYSKKYKL----- 1343 WP_031589969 1284 -CN----II QILATLHCNSSiGKIMYSDf--KI-sTTIg-R-LNGRTISL DISFIAESPTGMYSKKYKL----- 1344 KDA45870 1254 LGK---NFV ELLRYTADGAA-SDFKFFG--ENI--PRK--R-YNSAGSLL NGTLIYQSKTGLYETRIDLGKL-- 1314 WP_039099354 1307 ILDr----V -LIGLHANAAV-SDLGVLKisTPL--GKM--Q---QPSGIS DTQIIYQSPTGLFERRVALRDL-- 1368 AKP02966 1297 INSl-eELI ILLHANSTSAH-LIFNNIE-kKAF--GRK-------THGLT DTDFIYQSVTGLYETRIHIE---- 1356 WP_010991369 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--TTI--ERK--R-YNNLKELL NSTIIYQSITGLYESRKRL----- 1332 WP_033838504 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--TTI--ERK--R-YNNLKELL NSTIIYQSITGLYESRKRL----- 1332 EHN60060 1278 IAQ---SFV DLMAFNAMGAP-ASFKFFE--TTI--ERK--R-YNNLKELL NSTIIYQSITGLYESRKRL----- 1335 EFR89594 1044 IAQ---SFV DLMAFNAMGAP-ASFKFFE--TTI--ERK--R-YNNLKELL NSTIIYQSITGLYESRKRL----- 1101 WP_038409211 1275 IAK---SFD KLKVFNAFGAP-RDFEFFE--TTI--KRK--R-YYNIKELL NATIIYQSITGLYEARKRL----- 1332 EFR95520 894 IAK---SFD KLKVFNAFGAP-RDFEFFE--TTI--KRK--R-YYNIKELL NATIIYQSITGLYEARKRL----- 951 WP_003723650 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1332 WP_003727705 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1332 WP_003730785 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1332 WP_003733029 1275 IAE---SFV SLKKFNAFGVH-QDFSFFG--TKI--ERK--R-DRKLNELL NSTIIYQSITGLYESRKRL----- 1332 WP_003739838 1275 IAQ---SFV NLMAFNAMGAP-ASFKFFE--ATI--ERK--R-YTNLKELL SATIIYQSITGLYEARKRL----- 1332 WP_014601172 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1332 WP_023548323 1275 IAE---SFV SLKKFNAFGVH-KDFNFFG--TTI--KRK--R-DRKLKELL NSTIIYQSITGLYESRKRL----- 1332 WP_031665337 1275 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1332 WP_031669209 1275 IAE---SFV SLKKFNAFGVH-QDFSFFG--TKI--ERK--R-DRKLNELL NSTIIYQSITGLYESRKRL----- 1332 WP_033920898 1275 IAE---SFV SLKKFNAFGVH-KDFNFFG--TTI--KRK--R-DRKLKELL NSTIIYQSITGLYESRKRL----- 1332 AKI42028 1278 IAQ---SFV DLMAFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 1335 AKI50529 1278 IAE---SFV SLKKFNAFGVH-KDFNFFG--TTI--KRK--R-DRKLKELL NSTIIYQSITGLYESRKRL----- 1335 EFR83390 723 IAQ---SFV DLMVFNAMGAP-ASFKFFE--ATI--DRK--R-YTNLKELL SSTIIYQSITGLYESRKRL----- 780 WP_046323366 1275 IAQ---SFV NLLEFNAMGAP-ASFKYFE--TNI--ERK--R-YNNLKELL NATIIYQSITGLYEARKRL----- 1332 AKE81011 1322 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1381 CUO82355 1273 -AN----VI QMLIIMHKGPQnGNIIYDDf--NV-gKRIg-R-LNGRTFYL NIEFISQSPIGIYIKKYKL----- 1333 WP_033162887 1275 -CD----VI QILIIMHAGPMnGNIMYDDf--KF-tNRIg-R-FTHKNIDL KTTFISTSVTGLFSKKYKL----- 1335 AGZ01981 1339 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1398 AKA60242 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 AKS40380 1306 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1365 4UN5_B 1310 -AE---NII HLFTLTNLGAP-AAFKYFD--TTI--DRK--R-YTSTKEVL DATLIHQSITGLYETRIDLSQL-- 1369 WP_010922251 1366 GGD 1368 WP_039695303 1370 GEE 1372 WP_045635197 1368 GED 1370 5AXW_A --- WP_009880683 1050 GGD 1052 WP_010922251 1366 GGD 1368 WP_011054416 1366 GGD 1368 WP_011284745 1366 GGD 1368 WP_011285506 1366 GGD 1368 WP_011527619 1366 GGD 1368 WP_012560673 1366 GGD 1368 WP_014407541 1365 GGD 1367 WP_020905136 1366 GGD 1368 WP_023080005 1365 GGD 1367 WP_023610282 1365 GGD 1367 WP_030125963 1366 GGD 1368 WP_030126706 1366 GGD 1368 WP_031468318 1366 GGD 1368 WP_032460140 1366 GGD 1368 WP_032461047 1366 GGD 1368 WP_032462016 1366 GGD 1368 WP_032462936 1366 GGD 1368 WP_032464890 1366 GGD 1368 WP_033888930 1191 GGD 1193 WP_038431314 1366 GGD 1368 WP_038432938 1365 GGD 1367 WP_038434062 1366 GGD 1368 BAQ51233 1277 GGD 1279 KGE60162 541 GGD 543 KGE60856 304 GGD 306 WP_002989955 1366 GGD 1368 WP_003030002 1343 GED 1345 WP_003065552 1371 GEE 1373 WP_001040076 1368 GED 1370 WP_001040078 1376 GED 1378 WP_001040080 1368 GED 1370 WP_001040081 1368 GED 1370 WP_001040083 1368 GED 1370 WP_001040085 1368 GED 1370 WP_001040087 1368 GED 1370 WP_001040088 1368 GGD 1370 WP_001040089 1368 GED 1370 WP_001040090 1368 GED 1370 WP_001040091 1368 GED 1370 WP_001040092 1368 GED 1370 WP_001040094 1368 GED 1370 WP_001040095 1368 GEG 1370 WP_001040096 1368 GEG 1370 WP_001040097 1368 GED 1370 WP_001040098 1368 GED 1370 WP_001040099 1368 GED 1370 WP_001040100 1368 GED 1370 WP_001040104 1368 GED 1370 WP_001040105 1368 GED 1370 WP_001040106 1368 GED 1370 WP_001040107 1368 GED 1370 WP_001040108 1368 GED 1370 WP_001040109 1368 GED 1370 WP_001040110 1368 GED 1370 WP_015058523 1368 GED 1370 WP_017643650 1368 GED 1370 WP_017647151 1368 GED 1370 WP_017648376 1368 GED 1370 WP_017649527 1368 GED 1370 WP_017771611 1368 GED 1370 WP_017771984 1368 GED 1370 CFQ25032 1368 GED 1370 CFV16040 1368 GED 1370 KLJ37842 1368 GED 1370 KLJ72361 1368 GGD 1370 KLL20707 1382 GED 1384 KLL42645 1368 GED 1370 WP_047207273 1368 GED 1370 WP_047209694 1368 GED 1370 WP_050198062 1368 GED 1370 WP_050201642 1368 GED 1370 WP_050204027 1368 GED 1370 WP_050881965 1368 GED 1370 WP_050886065 1368 GED 1370 AHN30376 1368 GED 1370 EAO78426 1368 GED 1370 CCW42055 1368 GED 1370 WP_003041502 1384 GED 1386 WP_037593752 1344 GED 1346 WP_049516684 1344 GED 1346 GAD46167 1343 GED 1345 WP_018363470 1375 GEE 1377 WP_003043819 1373 GGD 1375 WP_006269658 1343 GED 1345 WP_048800889 1363 GED 1365 WP_012767106 1369 GGD 1371 WP_014612333 1369 GGD 1371 WP_015017095 1369 GGD 1371 WP_015057649 1369 GGD 1371 WP_048327215 1369 GGD 1371 WP_049519324 1369 GGD 1371 WP_012515931 1346 GEN 1348 WP_021320964 1346 GEN 1348 WP_037581760 1346 GEN 1348 WP_004232481 1375 GEE 1377 WP_009854540 1368 GEE 1370 WP_012962174 1369 GEE 1371 WP_039695303 1370 GEE 1372 WP_014334983 1373 GEE 1375 WP_003099269 1366 GGK 1368 AHY15608 --- AHY17476 --- ESR09100 198 GGK 200 AGM98575 --- ALF27331 1343 GGD 1345 WP_018372492 1351 GEN 1353 WP_045618028 1374 GED 1376 WP_045635197 1368 GED 1370 WP_002263549 1343 GGD 1345 WP_002263887 1343 GGD 1345 WP_002264920 1343 GGD 1345 WP_002269043 1343 GGD 1345 WP_002269448 1343 GGD 1345 WP_002271977 1343 GGD 1345 WP_002272766 1343 GGD 1345 WP_002273241 1343 GGD 1345 WP_002275430 1343 GGD 1345 WP_002276448 1343 GGD 1345 WP_002277050 1353 GGD 1355 WP_002277364 1343 GGD 1345 WP_002279025 1343 GGD 1345 WP_002279859 1343 GGD 1345 WP_002280230 1343 GGD 1345 WP_002281696 1343 GGD 1345 WP_002282247 1353 GGD 1355 WP_002282906 1343 GGD 1345 WP_002283846 1343 GGD 1345 WP_002287255 1343 GGD 1345 WP_002288990 1343 GGD 1345 WP_002289641 1343 GGD 1345 WP_002290427 1343 GGD 1345 WP_002295753 1343 GGD 1345 WP_002296423 1343 GGD 1345 WP_002304487 1357 GGD 1359 WP_002305844 1343 GGD 1345 WP_002307203 1343 GGD 1345 WP_002310390 1343 GGD 1345 WP_002352408 1343 GGD 1345 WP_012997688 1343 GGD 1345 WP_014677909 1343 GGD 1345 WP_019312892 1343 GGD 1345 WP_019313659 1343 GGD 1345 WP_019314093 1343 GGD 1345 WP_019315370 1343 GGD 1345 WP_019803776 1343 GGD 1345 WP_019805234 1343 GGD 1345 WP_024783594 1343 GGD 1345 WP_024784288 1353 GGD 1355 WP_024784666 1343 GGD 1345 WP_024784894 1343 GGD 1345 WP_024786433 1353 GGD 1355 WP_049473442 1343 GGD 1345 WP_049474547 1343 GGD 1345 EMC03581 1336 GGD 1338 WP_000428612 1371 GED 1373 WP_000428613 1369 GED 1371 WP_049523028 1364 GEE 1366 WP_003107102 1335 GGD 1337 WP_054279288 1366 GGD 1368 WP_049531101 1374 GED 1376 WP_049538452 1374 GED 1376 WP_049549711 1376 GED 1378 WP_007896501 1372 GGD 1374 EFR44625 1324 GGD 1326 WP_002897477 1368 GEE 1370 WP_002906454 1375 GED 1377 WP_009729476 1369 GED 1371 CQR24647 1359 GGE 1361 WP_000066813 1373 GED 1375 WP_009754323 1369 GED 1371 WP_044674937 1362 GGD 1364 WP_044676715 1364 GGD 1366 WP_044680361 1364 GGD 1366 WP_044681799 1362 GGD 1364 WP_049533112 1384 GED 1386 WP_029090905 --- WP_006506696 --- AIT42264 1366 GGD 1389 WP_034440723 --- AKQ21048 1366 GGD 1384 WP_004636532 1330 -ED 1332 WP_002364836 1336 -VD 1337 WP_016631044 1287 -VD 1288 EMS75795 1077 GEQ 1079 WP_002373311 1336 -VD 1337 WP_002378009 1336 -VD 1337 WP_002407324 1336 -VD 1337 WP_002413717 1336 -VD 1337 WP_010775580 1338 -VD 1339 WP_010818269 1336 -VD 1337 WP_010824395 1336 -VD 1337 WP_016622645 1336 -VD 1337 WP_033624816 1336 -VD 1337 WP_033625576 1336 -VD 1337 WP_033789179 1336 -VD 1337 WP_002310644 --- WP_002312694 --- WP_002314015 --- WP_002320716 --- WP_002330729 --- WP_002335161 --- WP_002345439 --- WP_034867970 1334 GEQ 1336 WP_047937432 --- WP_010720994 1334 GEQ 1336 WP_010737004 1334 GEQ 1336 WP_034700478 1334 GEQ 1336 WP_007209003 --- WP_023519017 1327 GER 1330 WP_010770040 1333 -VD 1334 WP_048604708 1329 -GD 1330 WP_010750235 1337 GVQ 1339 AII16583 1405 GGD 1424 WP_029073316 --- WP_031589969 --- KDA45870 --- WP_039099354 --- AKP02966 --- WP_010991369 1333 -DD 1334 WP_033838504 1333 -DD 1334 EHN60060 1336 -DD 1337 EFR89594 1102 -DD 1103 WP_038409211 1333 -ED 1334 EFR95520 952 -ED 953 WP_003723650 1333 -DD 1334 WP_003727705 1333 -DD 1334 WP_003730785 1333 -DD 1334 WP_003733029 1333 -DN 1334 WP_003739838 1333 -DG 1334 WP_014601172 1333 -DD 1334 WP_023548323 1333 -DS 1334 WP_031665337 1333 -DD 1334 WP_031669209 1333 -DN 1334 WP_033920898 1333 -DS 1334 AKI42028 1336 -DD 1337 AKI50529 1336 -DS 1337 EFR83390 781 -DD 782 WP_046323366 1333 -DD 1334 AKE81011 1382 GGD 1400 CUO82355 --- WP_033162887 --- AGZ01981 1399 GGD 1417 AKA60242 1366 GGD 1368 AKS40380 1366 GGD 1376 4UN5_B 1370 GGD 1372

Additional suitable Cas9 sequences in which amino acid residues homologous to residues 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and/or 1329 of SEQ ID NO: 9 can be identified are known to those of skill in the art. See, e.g., Supplementary Table S2 and Supplementary Figure S2 of Fonfara et al., Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems, Nucl. Acids Res. 2013, doi: 10.1093/nar/gkt1074, which are incorporated herein by reference in their entirety. Cas9 variants of the sequences provided herein or known in the art comprising one or more mutations, e.g., at least one, at least two, at least three, at least four, at least five, at least six, or at least seven mutations as provided herein, e.g., of one or more amino acid residue that is homologous to amino acid residue 10, 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 840, 1219, and/or 1329 in SEQ ID NO: 9 are provided by this disclosure, for example, Cas9 variants comprising a A262T, K294R, S409I, E480K, E543D, M694I, and/or E1219V mutation.

Example 4: Evolution of Cas9 with Broadened PAM Specificities

By evolving S. pyogenes Cas9 on a NNN PAM library using PACE, Cas9s with broadened PAM specificities have been evolved with higher activities for many non-canonical PAMs. Such a Cas9 still retains its native DNA binding and cutting activity and can be utilized with all of the current tools available. It has been hypothesized that by modulating Cas9's interactions with the DNA, the PAM specificity of Cas9 can be modified and expanded. Other Cas9s such as Staphylococcus aureus could also be engineered to change and expand their PAM specificity by such a method. Methods to modulate DNA binding such as targeted mutagenesis of the Cas9 protein, fusions to DNA-binding proteins, and the use of multiple Cas9 proteins tethered to each other could also expand the PAMs that can be targeted.

Cas9 Evolution.

After evolution using overnight propagation of the phage with the mutagenesis plasmid (MP), the resultant phages containing mutations, discussed above, in PACE were used. Twenty-four individual phages were sequenced from the PACE run. Mutations found in the Cas9 gene are documented in Table 3 below. The Cas9 gene containing these mutations was cloned out of the phage and into plasmids to test both DNA binding and cutting activity.

TABLE 3 Cas9 mutations pJH306 (WT dCas9- VPR) pJH407 (WT Cas9) pJH562 E480K E543D E1219V (dCas9-VPR) pJH599 A262T S409I E480K E543D M694I E1219V (dCas9-VPR) pJH600 K294R E480K E543D E1219V Q1256K L1362P (dCas9-VPR) pJH601 A262T S409I E480K E543D M694I E1219V (dCas9-VPR) pJH602 K294R E480K E543D E1219V Q1256K (dCas9-VPR) pJH603 A262T S409I E480K E543D M694I E1219V (dCas9-VPR) pJH604 S267G K294R E480K E543D E1219V N1224K Q1256K (dCas9-VPR) pJH605 A262T F405I S409I E480K E543D M694I E1219V (dCas9-VPR) pJH760 A262T S409I E480K E543D M694I E1219V (pJH599 Minus VPR, Nuclease Positive)

GFP Activation in Human Cell Culture.

Testing was performed on a reporter with a GFP reporter activated by dCas9-VPR. Testing was performed on 5′-NGG-3′ PAM first (FIGS. 4A and 4B) and then with a library of GFP reporters all containing the same Cas9 target site, but with NNN at the PAM position (FIGS. 5A and 5B). In this testing, dCas9-VPR was found to activate the GFP signal. Wild-type Cas9 with no mutations (pJH306), Cas9 evolved from overnight propagation (pJH562), and Cas9 evolved from PACE (pJH599-pJH605) were all tested. Mutations for each Cas9 are documented in Table 3 above.

dCas9-VPR on all 64 PAM Sequences.

pJH306 (WT dCas9-VPR) and pJH599 (WT dCas9-VPR with mutations A262T, S409I, E480K, E543D, M694I, and E1219V) were tested on all 64 PAM sequences (FIG. 6). dCas9-VPR was used to activate GFP as before and a different reporter plasmid was used for each well to ascertain the activation efficiency for all 64 different PAM sequences. The mean GFP fluorescence was measured for all transfected cells, as gated by iRFP signal. pJH599 showed either improved or similar levels of activation as compared to pJH306 for all PAM sequences.

In Vitro Cutting Assay.

The ability of expressed and purified WT Cas9 (WT) and Cas 9 with an E1219V mutation (1) to cut DNA with different PAMs was tested (FIG. 7). Cas9 was incubated with dsDNA containing the target site. Cutting was measured by running the DNA on a gel to compare the amount of uncut product to the amount of cut products, which run faster due to their smaller size. The E1219V mutation was found to increase Cas9 cutting activity on non-canonical PAMs while maintaining activity on 5′-NGG-3′ PAMs.

Evolving Different Systems.

In addition to S. pyogenes Cas9 evolution, other Cas9 systems such as S. aureus, S. thermophilus, N. meningitidis, and T. denticola, etc. can be evolved to modify and expand their PAM specificities. The data indicates that, by using a similar system to the S. pyogenes Cas9 evolution, a phage containing the S. aureus Cas9 can also be evolved to expand its PAM specificity.

Modulating PAM Specificity.

By mutating neutral and negatively charged amino acids to positively charged amino acids, Cas9 could be modified to expand the PAMs that can be targeted. Generally, incorporating mutations into Cas9 proteins that generate a net increase in positive charge may increase the affinity of Cas9 to bind DNA. In combination with the Cas9 mutations provided herein, additional residues that could be mutated for increased PAM targeting in S. pyogenes Cas9 further include ones that have been identified to change the PAM specificity (D1135, G1218, R1333, R1335, T1337)³⁸ and residues that can increase Cas9 activity (S845 and L847)³⁷. Residues that increase Cas9 specificity such as the mutations of arginine, histidine, and lysine to alanine previously identified³⁷ and the mutations of asparagine, arginine, and glutamine to alanine as previously identified³⁹ could lead to lower tolerability for non-canonical PAMs as these mutations presumably decrease the interactions between the Cas9 and DNA.

Fusions to Modulate PAM Specificity.

Programmable DNA-binding proteins such as zinc-finger domains, TALEs, and other Cas9 proteins could be fused to Cas9 to improve the ability to target nucleotide sequences having canonical or non-canonical PAMs, for example to increase activity, specificity or efficiency. A nuclease-null dCas9 could be fused to a nuclease active Cas9 to increase the ability of the nuclease active Cas9 to target different PAM sequences. One example of a nuclease-null dCas9 fused to a nuclease active Cas9 is shown in FIG. 8. Such fusions can be useful for improving the ability to target nucleotide sequences having canonical or non-canonical PAMs. The Cas9s could either be from the same species or from different species. Furthermore, both Cas9s could be nuclease-null dCas9, and could be further fused to effector proteins such as VP64, VP64-p65-Rta, FokI, GFP and other fluorescent proteins, deaminases, or any of the effector proteins provided herein.

Using Cas9 to Localize Other Nucleases and Other DNA-Binding Proteins.

Cas9 can also be used to overcome the native binding specificity of other proteins by localizing them to their DNA targets. DNA nucleases, recombinases, deaminases, and other effectors often have a native DNA specificity. Cas9 can be fused to these proteins to overcome and expand their native DNA specificities. gRNAs will target the Cas9 adjacent to the DNA effector's target site and will help to localize them to their target site.

dCas9-VPR on NNNNN PAM Library.

To test that the evolved Cas9s had not picked up specificity in the 4th and 5th PAM positions, dCas9-VPR on a NNNNN PAM library was tested. As seen with the NNN library, most of the constructs (e.g., pJH562, pJ559, pJH600, pJH601, pJH602, pJH603, and pJH605) showed improved activity. pJH599 consistently showed improvement in both the percentage of cells that showed GFP activation (FIG. 9A) and the mean fluorescence of those cells that showed GFP activation (FIG. 9B).

Cas9 Gfp Cutting.

The WT Cas9, pJH407, was compared with a nuclease-positive evolved Cas9, pJH760 (FIG. 10). pJH760 contained the same mutations as pJH599 but without the D10A and H480A nuclease-inactivating mutations, and without the VPR fusion. A genomically integrated GFP gene was cut by Cas9 and the activity was measured by the loss of GFP signal in cells. On a site with 5′-NGG-3′ PAM, pJH407 and pJH760 showed comparable activity. On a site with GAT PAM, pJH760 showed a significant increase in activity as compared to pJH407.

Example 5 Cas9:DNA Editing Enzyme Fusion Proteins

This disclosure further provides Cas9 fused with a DNA editing enzyme for the targeted editing of DNA sequences. FIG. 11 illustrates double stranded DNA substrate binding by DNA editing enzyme-dCas9:sgRNA complexes. The DNA editing enzyme shown is a deaminase. The structures according to FIG. 11 are identified in these sequences (36 bp: underlined, sgRNA target sequence: bold; PAM: boxed; 21 bp: italicized).

Example 6 PAM Depletion Assay

In E. coli, a library of PAM sequences are encoded in a plasmid that also contains an antibiotic gene. If the Cas9 can cut the PAM sequences on the plasmid, the plasmid is not replicated and is lost; only plasmids that are uncut remain the population. The plasmids that were cut by the Cas9 can be determined by sequencing the initial plasmid population and the final plasmid population through high throughput sequencing. The proportion of the library consisting of each PAM sequence was obtained by dividing the number of reads containing the PAM sequence by the total number of reads. The depletion score was then calculated by dividing the proportion of the library containing the PAM section before the selection by the proportion of the library containing the PAM sequence after selection. Higher depletion scores signify higher cutting activity by the Cas9 of that particular PAM sequence. The results of the PAM depletion assay are shown in FIG. 12.

A number of PAM sequences that were not cut with the wild-type Cas9 were cut with the evolved Cas9 (xCas9 v1.0, pJH760). Notably, all PAM sequences of the form NGN and NNG, as well as GAA and GAT showed a depletion greater than 10-fold with the xCas9. A single G in either the second or third PAM position could be sufficient for cutting with the newly evolved Cas9, opening up the sequence space significantly for the target sites that can be targeted using Cas9. The PAM depletion scores are given in Table 4.

TABLE 4 PAM Depletion Scores PAM xCas9 Sequence WT Cas9 v1.0 TCG 0.96 32.75 CAG 12.63 32.01 GCG 1.38 31.14 ACG 1.12 30.15 TGG 18.99 30.13 TGT 1.01 28.37 GGC 11.78 28.17 AGA 1.25 27.80 GAG 19.47 27.71 GGT 12.22 27.59 CGA 1.27 27.53 CGT 0.97 27.30 TGA 1.72 27.02 GGA 16.03 26.90 AGG 17.80 26.19 TAG 9.54 25.98 CGC 1.76 25.88 AGT 0.97 24.88 CGG 19.03 24.77 AAG 15.40 24.16 GTG 3.76 23.71 ATG 1.26 23.48 GGG 17.59 23.01 TGC 1.71 23.00 TTG 0.98 22.15 GAA 1.04 20.96 AGC 1.43 16.85 GAT 1.02 16.09 CTG 1.29 13.88 CCG 1.02 11.86 CAT 0.99 5.75 CAA 1.00 5.28 GAC 0.98 3.96 TAA 0.97 1.90 AAA 1.02 1.81 TAT 0.95 1.56 CAC 1.01 1.48 GTA 0.94 1.42 AAT 0.98 1.39 GCA 0.98 1.22 ACA 0.95 1.13 AAC 1.08 1.09 TAC 0.92 0.95 ATA 1.04 0.94 GTC 0.99 0.92 GCT 0.99 0.87 TCA 0.94 0.84 CTA 0.95 0.83 GCC 0.99 0.82 GTT 0.97 0.81 TCC 0.98 0.80 ACC 0.96 0.80 CTC 0.92 0.80 CCA 0.97 0.79 CCC 0.95 0.79 CTT 0.96 0.79 ATC 0.96 0.78 CCT 0.94 0.78 ATT 0.93 0.77 ACT 0.89 0.77 TTT 0.94 0.77 TCT 0.95 0.77 TTC 0.96 0.76 TTA 0.94 0.76

Example 7. GFP Cutting Mammalian Cells

Wild-type or evolved Cas9 and gRNAs were transfected into mammalian cells containing a genomically integrated GFP gene. Different gRNAs targeted different sites with different PAM sequences, such that cutting of the GFP by Cas9 would lead to a loss of the GFP signal. The GFP signal was quantified after five days with flow cytometry. As shown in FIG. 13, evolved Cas9 cut GFP in mammalian, in line with the results in the GFP activation assay and in the PAM depletion assay. High throughput sequencing around the cut site verified the results seen with the flow cytometer; the indels were proportional to the cutting percentage seen with the flow cytometer.

Example 8: Further Evoluation of the HHH Library

As SpCas9 has a preference for the G residues at the second and third base, evolution was continued using the end point from the last evolution on a HHH (H=A, C, or T) PAM library. After evolution, 13 colonies were sequenced and a number of new mutations were identified. Three mutations, the E1219V, E480K, and E543D mutations were found in all the clones. A number of the clones either had the S267G/K294R/Q1256K mutations or the A262T/S409I mutations but those mutations were never seen together, suggesting that the clones had taken two divergent paths along the evolutionsary landscape. The new mutations are given in Table 5.

TABLE 5 New Mutations HHH HHH HHH HHH HHH HHH HHH HHH HHH HHH HHH HHH HHH #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 (xCas9 2.0) 2.1) 2.2) 2.3) 2.4) 2.5) 2.6) 2.7) 2.8) 2.9) 2.10) 2.11) 2.12) D23N E108G R115H K141Q D180N P230S P230S D257N A262T A262T A262T S267G S267G S267G S267G S267G S267G S267G D284N K294R K294R K294R K294R K294R K294R K294R K294R R324L Q394H S409I S409I S409I S409I L455F T466A T474I E480K E480K E480K E480K E480K E480K E480K E480K E480K E480K E480K E480K E480K E543D E543D E543D E543D E543D E543D E543D E543D E543D E543D E543D E543D E543D K554R R654L M694I M694I M694I A711E L727P M763I I1063V V1100I E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V K1244N Q1256K Q1256K Q1256K Q1256K Q1256K Q1256K Q1256K Q1256K K1289Q A1323S

Example 9: Further Evolved Cas9 Proteins for Gene Editing

pJH760, described in Example 6, was tested in the PAM depletion assay on a number of new targets. Four new targets were selected: re2 (GGGGCCACTAGGGACAGGAT (SEQ ID NO: 314)), a synthetic target previously used for GFP activation in mammalian cells; VEGF (GGGTGGGGGGAGTTTGCTCC (SEQ ID NO: 315)), a target within the VEGF gene, CLTA (GCAGATGTAGTGTTTCCACA (SEQ ID NO: 316)), a target within the CLTA gene; and CCR5D (TCACTATGCTGCCGCCCAGT (SEQ ID NO: 317)), a target within the CCR5D gene. The results of the PAM depletion assay are given in FIGS. 14 to 17. It was found that, in addition to the canonical NGG sequence, PAM depletion demonstrates cutting for most NGN and some NNG sequences as well.

The HHH PAM library was further evolved using the end point from the last evolution on a HHH (H=A, C, or T) PAM library. After evolution, 13 colonies were sequenced and a number of new mutations were identified. Three mutations, the E1219V, E480K, and E543D mutations were found in all the clones. A number of the clones either had the K294R/Q1256K mutations or the A262T/S409I mutations but those mutations were never seen together, suggesting that the clones had taken two divergent paths along the evolutionsary landscape. The new mutations are given in Tables 8 and 9 below.

As expected, variation in activity was seen with different targets. The PAM depletion assay scores are given in Table 10. NGN consistently showed cutting activity with some targets. Variation was seen amoungst the xCas9 3.x mutants in terms of which mutant had the best activity. Of note, xCas9 3.3 contained the K294R/Q1256K series of mutations, while the other three mutants (3.6, 3.7, and 3.8) contained the A262T/S409I series of mutations. xCas9 3.6 and 3.7 outperformed 3.8. While 3.3 seemed to have the highest activity for most cases, 3.6 and 3.7 performed better on certain PAM sequences. The results of the PAM depletion assays for three of the new targets described above are given in FIGS. 18 to 20.

A NNNNN PAM depletion library was constructed. It was assayed to examine any fourth or fifth base specificity. Initial results of the PAM depletion assay show that there is no preference at the fourth and fifth base as expected.

In summary, E1219V was found to be one of the earliest mutations that fixed in the evoluation. It is close to the PAM sequence in the crystal structure. E480K and E543D were also seen in all of the clones from the early stages of the evolution, and may be important. K294R/Q1256K and A262T/S409I seem to be two divergent paths, and may be important. Their PAM sequence profiles seem to be slightly different, which implies their importance relative to PAM activity determination.

TABLE 6 Diseases/disorders containging T to C Changes.The table includes human gene mutations that may be corrected by changing a cytosine (C) to a thymine (T). The gene name, gene symbol,and Gene ID are indicated. SEQ ID Gene Gene NO:  Name ID Symbol Flanks 674 NM_000410.3(HFE): c.314T > C 3077 HFE [′ATGTTCACTGTTGACTTCTGGACTAYTATGG (p.Ile105Thr) AAAATCACAACCACAGCAAG′] 675 NM_144631.5(ZNF513): c.1015T > 130557 ZNF513 [′GCTGGGAGCTGCCATGTGTGGGCGCYGCAT C (p.Cys339Arg) GCGAGGAGAGGCTGGAGGGGG′] 676 NM_001199107.1(TBC1D24): c. 57465 TBC1D24 [′CGTGGCGCTGGCCATCCTCAAGTTCYTCCAC 751T > C (p.Phe251Leu) AAGGTGAGGGCCGGGCAGCC′] 677 NM_001708.2(OPN1SW): c.640T > 611 OPN1SW [′CATCTTCTGCTTCATTGTGCCTCTCBCCCTCA C (p.Ser214Pro) TCTGCTTCTCCTACACTCA′] 678 NM_000235.3(LIPA): c.599T > C 3988 LIPA [′AAAAGGATTAAAATGTTTTTTGCCCYGGGTC (p.Leu200Pro) CTGTGGCTTCCGTCGCCTTC′] 679 NM_000071.2(CBS): c.833T > C 875 CBS [′CTGAAGCCGCGCCCTCTGCAGATCAYTGGG (p.Ile278Thr) GTGGATCCCGAAGGGTCCATC′] 680 NM_000071.2(CBS): c.1616T > C 875 CBS [′TTCGGGGTGGTCACCGCCATTGACTYGCTGA (p.Leu539Ser) ACTTCGTGGCCGCCCAGGAG′] 681 NM_033409.3(SLC52A3): c.670T > 113278 SLC52A3 [′CGCCCACTTCTCACCCCTGGTCTTCYTCCTCC C (p.Phe224Leu) TCCTATCCATCATGATGGC′] 682 NM_033409.3(SLC52A3): c.1238T > 113278 SLC52A3 [′CTTTTCAGCGGCTGCCTCAGTTACGYCAAGG C (p.Val413Ala) TGATGCTGGGCGTGGTCCTG′] 683 NM_000274.3 (OAT): c.163T > C 4942 OAT [′GGAATATAAGTATGGTGCACACAACYACCA (p.Tyr55His) TCCTTTACCTGTAGCCCTGGA′] 684 NM_000274.3(OAT): c.1205T > C 4942 OAT [′TGTCTACGACTTCGAGATAATGGACYTCTGG (p.Leu402Pro) CCAAGCCAACCCATGGCGAC′] 685 NM_000274.3(OAT): c.1180T > C 4942 OAT [′TTTAGATTGGGATGCTTGGAAGGTGYGTCTA (p.Cys394Arg) CGACTTCGAGATAATGGACT′] 686 NM_001385.2(DPYS): c.1078T > 1807 DPYS [′TGTTGAAGATCGGATGTCCGTAATAYGGGA C (p.Trp360Arg) AAAAGGCGTGGTGGGTTTCAC′] 687 NM_000027.3(AGA): c.916T > C 175 AGA [′TCCAGAATTCTTTGGGGCTGTTATAYGTGCC (p.Cys306Arg) AATGTGACTGGAAGTTACGG′] 688 NM_000027.3(AGA): c.214T > C 175 AGA [′GTGTGAGAGAGAGCAGTGTGACGGCYCTGT (p.Ser72Pro) AGGCTTTGGAGGAAGTCCTGA′] 689 NM_000398.6(CYB5R3): c.382T > 1727 CYB5R3 [′CAAGTTTCCCGCTGGAGGGAAGATGYCTCA C (p.Ser128Pro) GTACCTGGAGAGCATGCAGAT′] 690 NM_000398.6(CYB5R3): c.446T > 1727 CYB5R3 [′GAGTTCCGGGGCCCCAGTGGGCTGCYGGTC C (p.Leu149Pro) TACCAGGGCAAAGGTGATTCG′] 691 NM_000398.6(CYB5R3): c.610T > 1727 CYB5R3 [′GAAGGACCCTGATGACCACACTGTGYGCCA C (p.Cys204Arg) CCTGCTCTTTGCCAACCAGGT′] 692 NM_000398.6(CYB5R3): c.218T > 1727 CYB5R3 [′CCGTCACCCCAGCACATCCTGGGCCYCCCTG C (p.Leu73Pro) TCGGTGAGTCACGCCCCTGC′] 693 NM_000552.3(VWF): c.4883T > C 7450 VWF [′CCTGGAGACATCCAGGTGGTGCCCAYTGGA (p.Ile1628Thr) GTGGGCCCTAATGCCAACGTG′] 694 NM_000552.3(VWF): c.4837T > C 7450 VWF [′CTACATGGTCACCGGAAATCCTGCCYCTGAT (p.Ser1613Pro) GAGATCAAGAGGCTGCCTGG′] 695 NM_000552.3(VWF): c.3814T > C 7450 VWF [′GGAACCGCCGTTGCACGATTTCTACBGCAGC (p.Cys1272Arg) AGGCTACTGGACCTGGTCTT′] 696 NM_000552.3(VWF): c.8317T > C 7450 VWF [′CAACGATGTGCAGGACCAGTGCTCCYGCTG (p.Cys2773Arg) CTCTCCGACACGGACGGAGCC′] 697 NM_000552.3(VWF): c.3445T > C 7450 VWF [′TGAGTGTGAGTGGCGCTATAACAGCYGTGC (p.Cys1149Arg) ACCTGCCTGTCAAGTCACGTG′] 698 NM_000552.3(VWF): c.3178T > C 7450 VWF [′GAAGCAGACGATGGTGGATTCCTCCYGTAG (p.Cys1060Arg) AATCCTTACCAGTGACGTCTT′] 699 NM_001042492.2(NF1): c.5858T > 4763 NF1 [′TGTTTGGAATACATGACTCCATGGCYGTCAA C (p.Leu1953Pro) ATCTAGTTCGTTTTTGCAAG′] 700 NM_000267.3(NF1): c.1523T > C 4763 NF1 [′ATTCATGCAGATCCAAAGCTCTTGCYTTGTG (p.Leu508Pro) TAAGTATTTTTTTATGAAAT′] 701 NM_000267.3(NF1): c.6200T > C 4763 NF1 [′ATTTTAGCACGCTACATGCTGATGCYGTCCT (p.Leu2067Pro) TCAACAATTCCCTTGATGTG′] 702 NM_000267.3(NF1): c.1070T > C 4763 NF1 [′CTGTTGGGGTTTTTATAGAACCTGCYTTTTA (p.Leu357Pro) ATCCAAGTAAGCCATTCTCA′] 703 NM_000267.3(NF1): c.3728T > C 4763 NF1 [′TCTCAGGATGAACTAGCTCGAGTTCYGGTTA (p.Leu1243Pro) CTCTGTTTGATTCTCGGCAT′] 704 NM_000308.2(CTSA): c.247T > C 5476 CTSA [′CTCCGGCTCCAAGCACCTCCACTACYGGTCT (p.Trp83Arg) GCCGCCCTGCCTTCTGGGCG′] 705 NM_001127695.1(CTSA): c.707T > 5476 CTSA [′ATCTCCTACAGGCTTTGGTCTTCTCYCCAGA C (p.Leu236Pro) CCCACTGCTGCTCTCAAAAC′] 706 NM_000308.2(CTSA): c.1271T > 5476 CTSA [′GATGTAGACATGGCCTGCAATTTCAYGGGG C (p.Met424Thr) GATGAGTGGTTTGTGGATTCC′] 707 NM_000405.4(GM2A): c.412T > 2760 GM2A [′GCGTACCTATGGGCTTCCTTGCCACYGTCCC C (p.Cys138Arg) TTCAAAGAAGTAAGTACTTA′] 708 NM_001165974.1(UROC1): c.209T > 131669 UROC1 [′CCAGAGTTTGCCCAGGAGCTGCAACYGTAC C (p.Leu70Pro) GGACACATCTACATGTACCGG′] 709 NM_003730.4(RNASET2): c.550T > 8635 RNASET2 [′ATATGGAGTGATACCCAAAATCCAGYGCCT C (p.Cys184Arg) TCCACCAAGCCAGGTTAGACA′] 710 NM_199069.1(NDUFAF3): c.2T > 25915 NDUFAF3 [′GACTTCGCCGCGCGTTGGTCAGCCAYGGCC C (p.Met1Thr) ACCGCTCTCGCGCTACGTAGC′] 711 NM_178012.4(TUBB2B): c.514T > 347733 TUBB2B [′CATGAACACCTTCAGCGTCATGCCCYCACCC C (p.Ser172Pro) AAGGTGTCAGACACGGTGGT′] 712 NM_178012.4(TUBB2B): c.683T > 347733 TUBB2B [′CCCACCTACGGGGACCTCAACCACCYGGTG C (p.Leu228Pro) TCGGCCACCATGAGCGGGGTC′] 713 NM_178012.4(TUBB2B): c.793T > 347733 TUBB2B [′CATGGTGCCCTTCCCTCGCCTGCACYTCTTC C (p.Phe265Leu) ATGCCCGGCTTCGCGCCCCT′] 714 NM_000110.3(DPYD): c.85T > C 1806 DPYD [′TCGAACACAAACTCATGCAACTCTGYGTTCC (p.Cys29Arg) ACTTCGGCCAAGAAATTAGA′] 715 NM_133459.3(CCBE1): c.520T > 147372 CCBE1 [′CATCCGGGAAGATGATGGGAAGACAYGTAC C (p.Cys174Arg) CAGGGGAGACAAATATCCCAA′] 716 NM_000035.3(ALDOB): c.442T > 229 ALDOB [′GAAAGATGGTGTTGACTTTGGGAAGYGGCG C (p.Trp148Arg) TGCTGTGCTGAGGATTGCCGA′] 717 NM_000320.2(QDPR): c.106T > C 5860 QDPR [′AACCAAAGCTGTTTTCTCCTTCCAGYGGGTT (p.Trp36Arg) GCCAGCGTTGATGTGGTGGA′] 718 NM_173560.3(RFX6): c.380 + 2T > 222546 RFX6 [′GCAGACACAGCTCACGCTGCAGTGGYGAGA C CTCGCCCGCAGGGTACACTGA′] 719 NM_173560.3(RFX6): c.649T > C 222546 RFX6 [′AAAATGTACTAATTTTTTAAGGTTTYCTGGA (p.Ser217Pro) AGCAAGCTAAAGAATGAGGT′] 720 NM_000037.3(ANK1): c.- 286 ANK1 [′CTGCGGGGCCTGTGACGTGCGGGCCRGGCC 108T > C CCCGAGGGCCTTATCGGCCCC′] 721 NM_001145308.4(LRTOMT): c.313T > 220074 LRTOMT [′TCACATCCTCACCACCCTGGACCACYGGAGC C (p.Trp105Arg) AGCCGCTGCGAGTACTTGAG′] 722 NM_001012515.2(FECH): c.1268T > 2235 FECH [′CCTGTCTGCAGGGAGACTAAATCCTYCTTCA C (p.Phe423Ser) CCAGCCAGCAGCTGTGACCC′] 723 NM_000140.3(FECH): c.315- 2235 FECH [′TATTGAGTAGAAAACATTTCTCAGGYTGCTA 48T > C AGCTGGAATAAAATCCACTT′] 724 NM_024120.4(NDUFAF5): c.686T > 79133 NDUFAF5 [′GCTGTCAATGACCTGGGACATCTGCYTGGG C (p.Leu229Pro) AGAGCTGGCTTTAATACTCTG′] 725 NM_000277.1(PAH): c.932T > C 5053 PAH [′TTACAGGAAATTGGCCTTGCCTCTCYGGGTG (p.Leu311Pro) CACCTGATGAATACATTGAA′] 726 NM_000277.1(PAH): c.764T > C 5053 PAH [′GGCCTGCTTTCCTCTCGGGATTTCTYGGGTG (p.Leu255Ser) GCCTGGCCTTCCGAGTCTTC′] 727 NM_000277.1(PAH): c.143T > C 5053 PAH [′TCACTCAAAGAAGAAGTTGGTGCATYGGCC (p.Leu48Ser) AAAGTATTGCGCTTATTTGAG′] 728 NM_000277.1(PAH): c.1045T > C 5053 PAH [′AAAGGCATATGGTGCTGGGCTCCTGBCATCC (p.Ser349Pro) TTTGGTGAATTACAGGTATG′] 729 NM_000277.1(PAH): c.293T > C 5053 PAH [GCTCTGACAAACATCATCAAGATCTYGAGG (p.Leu98Ser) CATGACATTGGTGCCACTGTC′] 730 NM_000277.1(PAH): c.734T > C 5053 PAH [′TGCACTGGTTTCCGCCTCCGACCTGHGGCTG (p.Val245Ala) GCCTGCTTTCCTCTCGGGAT′] 731 NM_000277.1(PAH): c.194T > C 5053 PAH [′GAGAATGATGTAAACCTGACCCACANTGAA (p.Ile65Thr) TCTAGACCTTCTCGTTTAAAG′] 732 NM_000130.4(F5): c.1160T > C 2153 F5 [′CATTTGGATAATTTCTCAAACCAAAYTGGAA (p.Ile387Thr) AACATTATAAGAAAGTTATG′] 733 NM_000512.4(GALNS): c.413T > 2588 GALNS [′AAGGCCGGCTACGTCAGCAAGATTGYCGGC C (p.Val138Ala) AAGTGGTAAGTCTCCTGGCCA′] 734 NM_018077.2(RBM28): c.1052T > 55131 RBM28 [′TCCTTTGACTCAGAAGAAGAAGAACYTGGG C (p.Leu351Pro) GAGCTTCTCCAACAGTTTGGA′] 735 NM_203395.2(IYD): c.347T > C 389434 IYD [′AATGAGCAAGTCCCAATGGAAGTCAYTGAT (p.Ile116Thr) AATGTCATCAGAACGGCAGGT′] 736 NM_015702.2(MMADHC): c.776T > 27249 MMADHC [′CATTTAGGATTCTCTGTTGATGACCYTGGAT C (p.Leu259Pro) GCTGTAAAGTGATTCGTCAT′] 737 NM_014165.3(NDUFAF4): c.194T > 29078 NDUFAF4 [′AAAGATGAAAAGCTGCTGTCGTTTCYAAAA C (p.Leu65Pro) GATGTGTATGTTGATTCCAAA′] 738 NM_001136271.2(NKX2- 137814 NKX2-6 [′GCAGGTGCTGGCCCTGGAGCGGCGCYTCAA 6): c.451T > C (p.Phe151Leu) GCAGCAGCGGTACCTGTCAGC′] 739 NM_013319.2(UBIAD1): c.511T > 29914 UBIAD1 [′TATCTACTTTGGAGGCCTGTCTGGCYCCTTT C (p.Ser171Pro) CTCTACACAGGAGGTAAGAT′] 740 NM_001127628.1(FBP1): c.581T > 2203 FBP1 [′TTGTCTAAAAAGGCCATCGGGGAGTYCATTT C (p.Phe194Ser) TGGTGGACAAGGATGTGAAG′] 741 NM_000046.3(ARSB): c.349T > C 411 ARSB [′TTTACAGCACCAAATAATCTGGCCCYGTCAG (p.Cys117Arg) CCCAGCTGTGTTCCTCTGGA′] 742 NM_000046.3(ARSB): c.707T > C 411 ARSB [′TCTTTCCAGCCTCTGTTTCTCTACCYTGCTCT (p.Leu236Pro) CCAGTCTGTGCATGAGCCC′] 743 NM_000404.2(GLB1): c.152T > C 2720 GLB1 [′AAGGATGGCCAGCCATTTCGCTACAYCTCA (p.Ile51Thr) GGAAGCATTCACTACTCCCGT′] 744 NM_000404.2(GLB1): c.247T > C 2720 GLB1 [′AAGGGGCTGTGTGTGTCTTGGCAGGBATGTG (p.Tyr83His) CCCTGGAACTTTCATGAGCC′] 745 NM_024782.2(NHEJ1) : c.367T > 79840 NHEJ1 [′CCTCCCCTTCTATTGGAATTTCCACYGCATG C (p.Cys123Arg) CTAGCTAGTCCTTCCCTGGT′] 746 NM_139241.3(FGD4): c.893T > C 121512 FGD4 [′CAGAAATTGGCACCATTCCTTAAGABGTATG (p.Met298Thr) GAGAATATGTGAAAGGATTT′] 747 NM_020631.4(PLEKHG5): c.1940T > 57449 PLEKHG5 [′TTGGGCCTTCCCTAACCAGGGTCCTYCCTCC C (p.Phe647Ser) TTATCTACCTGAATGAGTTT′] 748 NM_015559.2(SETBP1): c.2612T > 26040 SETBP1 [′GAGACGATCCCCAGCGACAGCGGCAYTGGG C (p.Ile871Thr) ACAGACAACAACAGCACTTCT′] 749 NM_138387.3(G6PC3): c.554T > 92579 G6PC3 [′CTCCTAGGCGCTGTCCTGGGCTGGCYGATGA C (p.Leu185Pro) CTCCCCGAGTGCCTATGGAG′] 750 NM_022489.3(INF2): c.556T > C 64423 INF2 [′CTTCAGCATTGTCATGAACGAGCTCYCCGGC (p.Ser186Pro) AGCGACAACGTGCCCTACGT′] 751 NM_022489.3(INF2): c.125T > C 64423 INF2 [′GACCCCGAGCTGTGCATCCGGCTGCYCCAG (p.Leu42Pro) ATGCCCTCTGTGGTCAACTAC′] 752 NM_018122.4(DARS2): c.492 + 2T > 55157 DARS2 [′TTTGAAATTAAGAACTTCGTGAAGGBACCA C ACCTCTGTTATTAATAAAATA′] 753 NM_015272.3(RPGRIP1L): c.1975T > 23322 RPGR1P1L [′CCTTCATCCCGAATATAACTTCACTYCTCAA C (p.Ser659Pro) TATCTTGTTCATGTTAATGA′] 754 NM_001029871.3(RSP04): c.319T > 343637 RSPO4 [′CTTCAGCCAGGACTTCTGCATCCGGYGCAAG C (p.Cys107Arg) AGGCAGTTTTACTTGTACAA′] 755 NM_000429.2(MAT1A): c.914T > 4143 MAT1A [′GCTGCCCGCTGGGTGGCCAAGTCTCYGGTG C (p.Leu305Pro) AAAGCAGGGCTCTGCCGGAGA′] 756 NM_032581.3(FAM126A): c.158T > 84668 FAM126A [′AACATTTTGTCTTTCTTCTAGTTGCBAGAAC C (p.Leu53Pro) CTGTCTGTCACCAGCTCTTT′] 757 NM_006364.2(SEC23A): c.1144T > 10484 SEC23A [′GGGTGATTCTTTCAATACTTCCTTAYTCAAA C (p.Phe382Leu) CAAACTTTTCAAAGAGTCTT′] 758 NM_018006.4(TRMU): c.229T > 55687 TRMU [′CCATCAAGTGTCCTACGTAAAGGAGYATTG C (p.Tyr77His) GAATGATGTGTTCAGGTGAGT′] 759 NM_001174089.1(SLC4A11):  83959 SLC4A11 [′CTGCAGGTGCTTCAGCTGCTGCTGCYGTGTG c.2480T > C (p.Leu827Pro) CCTTCGGCATGAGCTCCCTG′] 760 NM_001174089.1(SLC4A11): c.589T > 83959 SLC4A11 [′AGTGACAGGGGTGCGGTACCAGCAGYCGTG C (p.Ser197Pro) GCTCTGCATCATGTGAGTTGC′] 761 NM_148960.2(CLDN19): c.269T > 149461 CLDN19 [′GCCCTGATGGTGGTGGCCGTGCTCCYGGGCT C (p.Leu90Pro) TCGTGGCCATGGTCCTCAGC′] 762 NM_153704.5(TMEM67): c.2498T > 91147 TMEM67 [′AACACAGATGGTCAGACTTTTGAGAYTGCA C (p.Ile833Thr) ATTTCTAACCAGATGAGACAA′] 763 NM_153704.5(TMEM67): c.1769T > 91147 TMEM67 [′ACAGGTCTTTACTGGCTTATTTTCTYCAAAG C (p.Phe590Ser) TGAGTGAGTTTCTGAATTTT′] 764 NM_153704.5(TMEM67): c.1843T > 91147 TMEM67 [′AGAACGTTTTGTCACTTATGTTGGAHGTGCC C (p.Cys615Arg) TTTGCTCTGAAGGTAAGTTT′] 765 NM_153704.5(TMEM67): c.755T > 91147 TMEM67 [′ACATCTTGTCAAGCTCTTGGAAATAYGTGTG C (p.Met252Thr) TGATGAACATGAATTCTTAC′] 766 NM_017777.3(MKS1): c.80 + 2T > 54903 MKS1 [′CCCCGTGCGCAACTTGCGCCTCCGGYAGTCG C CACCGCCCCAGCCCCGAGGC′] 767 NM_001041.3(SI): c.1022T > C 6476 SI [′AATTACATGCTCATTTACTTTAAGCYTGTTG (p.Leu341Pro) GACTACCAGCAATGCCAGCA′] 768 NM_001041.3(SI): c.1859T > C 6476 SI [′ATGGAATGGTCTATAACTGGAATGCYGGAG (p.Leu620Pro) TTCAGTTTGTTTGGAATACCT′] 769 NM_015506.2(MMACHC): c.347T > 25974 MMACHC [′CACCCCAACCGACGCCCCAAGATCCYGGCC C (p.Leu116Pro) CAGACAGCAGCCCATGTAGCT′] 770 NM_000190.3(HMBS): c.739T > 3145 HMBS [′GCACGATCCCGAGACTCTGCTTCGCYGCATC C (p.Cys247Arg) GCTGAAAGGGCCTTCCTGAG′] 771 NM_000190.3(HMBS): c.242T > 3145 HMBS [′GAGAAAAGCCTGTTTACCAAGGAGCYTGAA C (p.Leu81Pro) CATGCCCTGGAGAAGAATGAG′] 772 NM_000237.2(LPL): c.662T > C 4023 LPL [′ACCAGAGGGTCCCCTGGTCGAAGCAYTGGA (p.Ile221Thr) ATCCAGAAACCAGTTGGGCAT′] 773 NM_000237.2(LPL): c.337T > C 4023 LPL [′AGACTCCAATGTCATTGTGGTGGACYGGCTG (p.Trp113Arg) TCACGGGCTCAGGAGCATTA′] 774 NM_000237.2(LPL): c.755T > C 4023 LPL [′AACATTGGAGAAGCTATCCGCGTGAYTGCA (p.Ile252Thr) GAGAGAGGACTTGGAGGTAAA′] 775 NM_000263.3(NAGLU): c.142T > 4669 NAGLU [′GCTGGGGCCAGGCCCCGCGGCCGACYTCTC C (p.Phe48Leu) CGTGTCGGTGGAGCGCGCTCT′] 776 NM_000018.3(ACADVL): c.1372T > 37 ACADVL [′GCGTGTGCTCCGAGATCTTCGCATCYTCCGG C (p.Phe458Leu) ATCTTTGAGGGGACAAATGA′] 777 NM__018105.2(THAP 1): c.241T > 55145 THAP1 [′GAAAGAGAATGCTGTGCCCACAATAYTTCTT C (p.Phe81Leu) TGTACTGAGCCACATGACAA′] 778 NM_005908.3(MANBA): c.1513T > 4126 MANBA [′AGACAAGAGTCGTCCTTTTATTACGYCCAGT C (p.Ser505Pro) CCTACAAATGGGGCTGAAAC′] 779 NM_000124.3(ERCC6): c.2960T > 2074 ERCC6 [′AAGCAGTTTTTGACAAATAGAGTGCYAAAA C (p.Leu987Pro) GACCCAAAACAAAGGCGGTTT′] 780 NM_014845.5(FIG4): c.122T > C 9896 FIG4 [′GAAACGAAATATCGTGTCTTGAAGAYTGAT (p.Ile41Thr) AGAACAGAACCAAAAGATTTG′] 781 NM_000787.3(DBH): c.339+2T > 1621 DBH [′GATGGGGACACTGCCTATTTTGCGGYGAGTC C TCTCCTCCCTGCCAGCTCTC′] 782 NM_000102.3(CYP17A1): c.316T > 1586 CYP17A1 [′TCTAAAGGCAACTCTAGACATCGCGYCCAA C (p.Ser106Pro) CAACCGTAAGGGTATCGCCTT′] 783 NM_000102.3(CYP17A1): c.1216T > 1586 CYP17A1 [′GGCGCTGCATCACAATGAGAAGGAGYGGCA C (p.Trp406Arg) CCAGCCGGATCAGTTCATGCC′] 784 NM_000102.3(CYP17A1): c.1358T > 1586 CYP17A1 [′GAGATCCTGGCCCGCCAGGAGCTCTYCCTCA C (p.Phe453Ser) TCATGGCCTGGCTGCTGCAG′] 785 NM_152783.4(D2HGDH): c.1331T > 728294 D2HGDH [′GGAGATGGTAACCTGCACCTCAATGYGACG C (p.Val444Ala) GCGGAGGCCTTCAGCCCCTCG′] 786 NM_000255.3(MUT): c.313T > C 4594 MUT [′TCCTACCATGTATACCTTTAGGCCCYGGACC (p.Trp105Arg) ATCCGCCAGTATGCTGGTTT′] 787 NM_000411.6(HLCS): c.710T > C 3141 HLCS [′CTGTACCAGAAGTTCATGGCCTATCYTTCTC (p.Leu237Pro) AGGGAGGGAAGGTGTTGGGC′] 788 NM_022132.4(MCCC2): c.499T > 64087 MCCC2 [′AATTGCCATGCAAAACAGGCTCCCCYGCAT C (p.Cys167Arg) CTACTTAGGCAAGTCACCAGA′] 789 NM_020166.4(MCCC1): c.1310T > 56922 MCCC1 [′CATTATGACCCCATGATTGCGAAGCYGGTCG C (p.Leu437Pro) TGTGGGCAGCAGATCGCCAG′] 790 NM_198578.3(LRRK2): c.6059T > 120892 LR12K2 [′ATTGCAAAGATTGCTGACTACGGCAYTGCTC C (p.Ile2020Thr) AGTACTGCTGTAGAATGGGG′] 791 NM_000022.2(ADA): c.320T > C 100 ADA [′GAGGTGCGGTACAGTCCGCACCTGCYGGCC (p.Leu107Pro) AACTCCAAAGTGGAGCCAATC′] 792 NM_199242.2(UNC13D): c.1208T > 201294 UNC13D [′CTGGCCGCCTCATTCAGCTCCCTGCYGACCT C (p.Leu403Pro) ACGGCCTCTCCCTCATCCGG′] 793 NM_152443.2(RDH12): c.523T > 145226 RDH12 [′CCCTGCACGGGTGGTTAATGTGTCCYCGGTG C (p.Ser175Pro) GCTCACCACATTGGCAAGAT′] 794 NM_020435.3(GJC2): c.857T > C 57165 GJC2 [′TGCCTGCTGCTCAACCTCTGTGAGAYGGCCC (p.Met286Thr) ACCTGGGCTTGGGCAGCGCG′] 795 NM_000159.3(GCDH): c.883T > 2639 GCDH [′CTTCGGCTGCCTGAACAACGCCCGGYACGG C (p.Tyr295His) CATCGCGTGGGGCGTGCTTGG′] 796 NM_000920.3(PC): c.434T > C 5091 PC [′CGGTTTATTGGGCCAAGCCCAGAAGBGGTC (p.Val145Ala) CGCAAGATGGGAGACAAGGTG′] 797 NM_207118.2(GTF2H5): c.62T > 404672 GTF2H5 [′GATCCTGCCATGAAGCAGTTTCTGCYGTACT C (p.Leu21Pro) TGGATGAGTCCAATGCCCTG′] 798 NM_005787.5(ALG3): c.211T > C 10195 ALG3 [′TCTTCTTCCAGACACAGAGATTGACYGGAA (p.Trp7lArg) GGCCTACATGGCCGAGGTAGA′] 799 NM_024514.4(CYP2R1): c.296T > 120227 CYP2R1 [′GGCTATGATGTAGTAAAGGAATGCCYTGTTC C (p.Leu99Pro) ATCAAAGCGAAATTTTTGCA′] 800 NM_017929.5(PEX26): c.2T > C 55670 PEX26 [′CTGGGCCTTGGACCCGGACTCGTTAYGAAG (p.Met1Thr) AGCGATTCTTCGACCTCTGCA′] 801 NM_017929.5(PEX26): c.134T > 55670 PEX26 [′CTTCTGGAGGAGGCGGCCGACCTCCYGGTG C (p.Leu45Pro) GTGCACCTGGACTTCCGGGCG′] 802 NM_213599.2(AN05): c.1066T > 203859 ANO5 [′TGGTCAGATGATCATGTGCCCACTCBGTGAT C (p.Cys356Arg) CAAGTGTGTGATTATTGGAG′] 803 NM_022370.3(ROBO3): c.2113T > 64221 ROBO3 [′CCAGCTGGTGCAAGGTTTCCGGGTGHCTTGG C (p.Ser705Pro) AGGGTAGCAGGCCCTGAGGG′] 804 NM_022370.3(ROBO3): c.14T > C 64221 ROBO3 [′CTGGGTCGAGCCATGCTGCGCTACCYGCTGA (p.Leu5Pro) AAACGCTGCTGCAGATGAAC′] 805 NM_207352.3(CYP4V2): c.332T > 285440 CYP4V2 [′GTATGTTTTTCTCTTCCTAAGGTAABTTTAAC C (p.Ile111Thr) TAGTTCAAAGCAAATTGAC′] 806 NM_178857.5(RP1L1): c.2878T > 94137 RP1L1 [′GTCTCCAGAGGCTGTGGTCCGCGAAYGGCT C (p.Trp960Arg) GGACAACATTCCAGAAGAGCC′] 807 NM_018668.4(VPS33B): c.89T > 26276 VPS33B [′GCTCGAGACCAGCTCATCTATCTGCYGGAGC C (p.Leu30Pro) AGGTCAGTGCTTGCCTGACG′] 808 NM_024006.5(VKORC1): c.134T > 79001 VKORC1 [′CGGGATTACCGCGCGCTCTGCGACGYGGGC C (p.Val45Ala) ACCGCCATCAGCTGTTCGCGC′] 809 NM_000551.3(VHL): c.334T > C 7428 VHL [′TGGCACGGGCCGCCGCATCCACAGCHACCG (p.Tyr112His) AGGTACGGGCCCGGCGCTTAG′] 810 NM_000551.3(VHL): c.292T > C 7428 VHL [′CAACTTCGACGGCGAGCCGCAGCCCYACCC (p.Tyr98His) AACGCTGCCGCCTGGCACGGG′] 811 NM_000551.3(VHL): c.188T > C 7428 VHL [′GAGGCCGGGCGGCCGCGGCCCGTGCYGCGC (p.Leu63Pro) TCGGTGAACTCGCGCGAGCCC′] 812 NM_000551.3(VHL): c.488T > C 7428 VHL [′GTGTATACTCTGAAAGAGCGATGCCYCCAG (p.Leu163Pro) GTTGTCCGGAGCCTAGTCAAG′] 813 NM_014874.3(MFN2): c.227T > C 9927 MFN2 [′CCCGTTACCACAGAAGAACAGGTTCBGGAC (p.Leu76Pro) GTCAAAGGTTACCTATCCAAA′] 814 NM_015046.5(SETX): c.1166T > 23064 SETX MACATGTATGAAGAAATGGAAACATYAGCC C (p.Leu389Ser) AGTGTACTTCAGTCAGATATT′] 815 NM_005609.2(PYGM): c.1187T > 5837 PYGM [′TGGCCGGTGCACCTCTTGGAGACGCYGCTGC C (p.Leu396Pro) CGCGGCACCTCCAGATCATC′] 816 NM_005609.2(PYGM): c.2392T > 5837 PYGM [′CTTTGACCTGCAGAACCCAAGAGAGHGGAC C (p.Trp798Arg) GCGGATGGTGATCCGGAACAT′] 817 NM_213653.3(HFE2): c.842T > C 148738 HFE2 [′CATGTGGAGATCCAAGCTGCCTACAYTGGC (p.Ile281Thr) ACAACTATAATCATTCGGCAG′] 818 NM_213653.3(HFE2): c.238T > C 148738 HFE2 [′TGGAGGGGTGGGCTCTGGCGGCCTCBGTCG (p.Cys80Arg) AGCCCTCCGCTCCTATGCGCT′] 819 NM_213653.3(HFE2): c.302T > C 148738 HFE2 [′ACCGCCCGCACCTGCCGCGGGGACCYCGCC (p.Leu101Pro) TTCCATTCGGCGGTACATGGC′] 820 NM_000045.3(ARG1): c.32T > C 383 ARG1 [′GCCAAGTCCAGAACCATAGGGATTAYTGGA (p.Ile11Thr) GCTCCTTTCTCAAAGGGACAG′] 821 NM_032409.2(PINK1): c.1040T > −1 — [′GCCGCCATGATGCTGCTGCAGCTGCYGGAA C (p.Leu347Pro) GGCGTGGACCATCTGGTTCAA′] 822 NM_153006.2(NAGS): c.1289T > −1 — [′GCCAGGTACAACGCCGCCGCCATTCYGACC C (p.Leu430Pro) ATGGAGCCCGTCCTGGGGGGC′] 823 NM_153006.2(NAGS): c.1450T > −1 — [′CCGGGTCACCAACCCCATCAATCCCBGGTAG C (p.Trp484Arg) GTCCTGCCACTCCCAGCTCT′] 824 NM_001173464.1(KIF21A): c.3029T > 55605 KIF21A [′AGTATTTCTGATTGTCAGGCCAACAYAATGC C (p.Ile1010Thr) AGATGGAAGAAGCAAAGGTT′] 825 NM_001173464.1(KIF21A): c.1067T > 55605 KIF21A [′GTCAGCCCTTCAGACAGAGACTTTAYGGAA C (p.Met356Thr) ACGTTAAACACCCTGAAATAC′] 826 NM_000434.3(NEU1): c.1088T > 4758 NEU1 [′TCATGGCGGAAAGAGACAGTCCAGCYATGG C (p.Leu363Pro) CCAGGCCCCAGTGGCTATTCA′] 827 NM_000434.3(NEU1): c.718T > C 4758 NEU1 [′CCTCAGCGATGATCATGGTGCCTCCYGGCGC (p.Trp240Arg) TACGGAAGTGGGGTCAGCGG′] 828 NM_000026.2(ADSL): c.1312T > 158 ADSL [′TGATGCCTACTTCAGTCCCATTCACYCCCAG C (p.Ser438Pro) TTGGATCATTTACTGGATCC′] 829 NM_000026.2(ADSL): c.674T > C 158 ADSL [′TCCAAGGTAGAGCAGCTTGACAAGAYGGTG (p.Met225Thr) ACAGAAAAGGCAGGATTTAAG′] 830 NM_018400.3(SCN3B): c.29T > C 55800 SCN3B [′CCTGCCTTCAATAGATTGTTTCCCCYGGCTT (p.Leu10Pro) CTCTCGTGCTTATCTACTGG′] 831 NM_024577.3(SH3TC2): c.505T > 79628 SH3TC2 [′CCTTCCTGTATCAGGAGTCCCAGGTRTATTG C (p.Tyr169His) TTTCCAGGTGTTTATCATCT′] 832 NM_000243.2(MEFV): c.2177T > 4210 MEFV [′GTGGGCATCTTCGTGGACTACAGAGHTGGA C (p.Val726Ala) AGCATCTCCTTTTACAATGTG′] 833 NM_000483.4(APOC2): c.142T > −1 — [′GGTGAAGGAATCTCTCTCCAGTTACYGGGA C (p.Trp48Arg) GTCAGCAAAGACAGCCGCCCA′] 834 NM_058172.5(ANTXR2): c.566T > 118429 ANTXR2 [′CTCTTCTTTCTAAAGCTTGAAAGAAYTGCTG C (p.Ile189Thr) ATTCCAAGGAGCAAGTTTTC′] 835 M_001128085.1(ASPA): c.454T > −1 — [′TCAGACTTCTCTGGCTCCACTACCCYGCTAC C (p.Cys152Arg) GTTTATCTGATTGAGCATCC′] 836 NM_022464.4(SIL1): c.645 + 2T > 64374 SIL1 [′TGACTTATGGACGAAGAAATACAGTRCCTG C ATGGACATAATATTCAAGATC′] 837 NM_001037633.1(SIL1): c.1370T > 64374 SIL1 [′CTGCTGGGCTCTGTCAACAGCTTGCYGAAGG C (p.Leu457Pro) AGCTGAGATGAGGCCCCACA′] 838 NM_000391.3(TPP1): c.1093T > C 1200 TPP1 [′TCTCTCAGGTGACAGTGGGGCCGGGYGTTG (p.Cys365Arg) GTCTGTCTCTGGAAGACACCA′] 839 NM_182760.3(SUMF1): c.1006T > 285362 SUMF1 [′AGTGAAGAAAGGTGGATCCTACATGYGCCA C (p.Cys336Arg) TAGGGTAAGTCATGTCACTAA′] 840 NM_182760.3(SUMF1): c.463T > 285362 SUMF1 [′CTTATAGGCTGAGAAGTTTGGCGACYCCTTT C (p.Ser155Pro) GTCTTTGAAGGCATGTTGAG′] 841 NM_177986.3(DSG4): c.574T > C −1 — [′TGCAGATGAAGAAAATCATCTGAATHCTAA (p.Ser192Pro) AATTGCCTACAAGATCGTCTC] 842 NM_004183.3(BEST1): c.253T > 7439 BEST1 [′CCCCCGCCCCTCCTGCCCAGGCTTCYACGTG C (p.Tyr85His) ACGCTGGTCGTGACCCGCTG′] 843 NM_004183.3(BEST1): c.122T > 7439 BEST1 [′TATGGCGAGTTCTTAATCTTCCTGCYCTGCT C (p.Leu41Pro) ACTACATCATCCGCTTTATT′] 844 NM_004183.3(BEST1): c.614T > 7439 BEST1 [′CTTGGAGGTCGAATCCGGGACCCTAYCCTGC C (p.Ile205Thr) TCCAGAGCCTGCTGAACGTG′] 845 NM_004183.3(BEST1): c.704T > 7439 BEST1 [′TACGACTGGATTAGTATCCCACTGGYGTATA C (p.Val235Ala) CACAGGTGAGGACTAGGCTG′] 846 NM_024312.4(GNPTAB): c.1120T > 79158 GNPTAB [′TGTTTGTTGTTGTTAAAAGGATGTTYTTCGA C (p.Phe374Leu) AATTTGAGCCACTTGCCTAC′] 847 NM_000158.3(GBE1): c.671T > C 2632 GBE1 [′TATAAACATTTTACATGCAATGTACYACCAA (p.Leu224Pro) GAATCAAAGGCCTTGGTAAG′] 848 NM_017890.4(VPS13B): c.8459T > 157680 VPS13B [′CAGGTGCCATCTTCAAACAGTTCCAYTATTT C (p.Ile2820Thr) ATGTCTGGTGCACAGTTTTG′] 849 NM_000019.3(ACAT1): c.935T > 38 ACAT1 [′CTCAATGTTACACCACTGGCAAGAAYAGTA C (p.Ile312Thr) GGTAAGGCCAGGCGAGGTGGC′] 850 NM_020184.3(CNNM4): c.971T > 26504 CNNM4 [′CTCAGTTTTCCCATTAGCAAGCTCCYGGACT C (p.Leu324Pro) TTTTTCTGGGCCAGGAGATT′] 851 NM_001171507.2(MCFD2): c.407T > 90411 MCFD2 [′GATGACAAGAACAATGATGGATACAYTGAC C (p.Ile136Thr) TATGCTGAATTTGCAAAATCA′] 852 NM_174936.3(PCSK9): c.646T > 255738 PCSK9 [′TGTGCCCGAGGAGGACGGGACCCGCYTCCA C (p.Phe216Leu) CAGACAGGTAAGCACGGCCGT′] 853 NM_000419.3(ITGA2B): c.1787T > 3674 ITGA2B [′GACTTCCGGGACAAGCTGAGCCCCAYTGTG C (p.Ile596Thr) CTCAGCCTCAATGTGTCCCTA′] 854 NM_000419.3(ITGA2B): c.641T > 3674 ITGA2B [′CCCTTCCAGGCCGGAGAGCTGGTGCYTGGG C (p.Leu214Pro) GCTCCTGGCGGCTATTATTTC′] 855 NM_004817.3(TJP2): c.143T > C 9414 TJP2 [′TCCAAAAGAGGATTTGGAATTGCAGYGTCC (p.Val48Ala) GGAGGCAGAGACAACCCCCAC′] 856 NM_173477.4(1JSH1G): c.143T > 124590 USH1G [′GCCTACCATGGCAACCTCGAGTCGCYGCGTC C (p.Leu48Pro) TCATTGTGAGCCGCGGGTGA′] 857 NM_000271.4(NPC1): c.3182T > 4864 NPC1 [′GACGCTCTGAAGAAAGCCCGACTTAYAGCC C (p.Ile1061Thr) AGTAATGTCACCGAAACCATG′] 858 NM_000271.4(NPC1): c.1133T > 4864 NPC1 [′TTTGTCCGGGTCACAACCAATCCAGYTGACC C (p.Val378Ala) TCTGGTCAGCCCCCAGCAGC′] 859 NM_000271.4(NPC1): c.337T > C 4864 NPC1 [′ACTGAACCTGTTTTGTGAGCTGACAYGTAGC (p.Cys113Arg) CCTCGACAGAGTCAGTTTTT′] 860 NM_000543.4(SMPD1): c.911T > 6609 SMPD1 [′CGGGCCCTGACCACCGTCACAGCACYTGTG C (p.Leu304Pro) AGGAAGTTCCTGGGGCCAGTG′] 861 NM_000226.3(KRT9): c.503T > C 3857 KRT9 [CTCAATTCTCGGCTGGCCTCTTACTYGGATA (p.Leu168Ser) AGGTGCAGGCTCTAGAGGAG′] 862 NM_000226.3(KRT9): c.470T > C 3857 KRT9 [′CTGACTGCTAATGAGAAGAGCACCANGCAG (p.Met157Thr) GAACTCAATTCTCGGCTGGCC′] 863 NM_000051.3(ATM): c.7967T > C −1 — [′CCAGCAGACCAGCCAATTACTAAACYTAAG (p.Leu2656Pro) AATTTAGAAGATGTTGTTGTC′] 864 NM_000487.5(ARSA): c.410T > C 410 ARSA [′GGGGTGGGGCCTGAGGGGGCCTTCCYGCCC (p.Leu137Pro) CCCCATCAGGGCTTCCATCGA′] 865 NM_017653.3(DYM): c.1624T > 54808 DYM [′AATGATGTTAGAGATCATCAACTCCYGCCTG C (p.Cys542Arg) ACAAATTCCCTTCACCACAA′] 866 NM_016038.2(SBDS): c.258 + 2T > 51119 SBDS [′GACCAAACTGAAATCTGTAAGCAGGYGGGT C AACAGCTGCAGCATAGCTAAC′] 867 NM_001079802.1(FKTN): c.527T > 2218 FKTN [′ACTCATGCGATCCACTTGGTAGTCTYTCATG C (p.Phe176Ser) AGAGGAGTGGCAACTACCTC′] 868 NM_013382.5(POMT2): c.2242T > 29954 POMT2 [′GGCAGGACTAAGGTGGCTGGACTCAYGGGA C (p.Trp748Arg) CTTTTGAGGCCACTGCAAAGA′] 869 NM_024529.4(CDC73): c.191T > 79577 CDC73 [′ACATTGGATTCCATTTTATTTCTACYTAATA C (p.Leu64Pro) ACGTGCACCTTTCTCATCCT′] 870 NM_000268.3(NF2): c.1079T > C 4771 NF2 [′ACGAGGGATGAGTTGGAGAGGAGGCYGCTG (p.Leu360Pro) CAGATGAAAGAAGAAGCAACA′] 871 NM_000268.3(NF2): c.1604T > C 4771 NF2 [′GAATACATGGAAAAGAGCAAGCATCYGCAG (p.Leu535Pro) GAGCAGCTCAATGAACTCAAG′] 872 NM_000268.3(NF2): c.185T > C 4771 NF2 [′CTGGGGCTCCGAGAAACCTGGTTCTYTGGAC (p.Phe62Ser) TGCAGTACACAATCAAGGAC′] 873 NM_139248.2(LIPH): c.322T > C 200879 LIPH [′AGACATGAACGTAGTTGTTGTTGATYGGAAT (p.Trp108Arg) CGAGGAGCTACAACTTTAAT′] 874 NM_015102.4(NPHP4): c.2972T > 261734 NPHP4 [′GCCACGCTGGGGGTCGCCGAGTTCTYTGAGT C (p.Phe991Ser) TTGTGCTTAAGAACCCCCAC′] 875 NM_024105.3(ALG12): c.473T > 79087 ALG12 [′ACCCTCCCGCACTGTCTTGCAGTCCYGCTGG C (p.Leu158Pro) CCCTCGCGGCCTGGCTGCGG′] 876 NM_000280.4(PAX6): c.773T > C 5080 PAX6 [′GTCCCACCTGATTTCCAGGTATGGTYTTCTA (p.Phe258Ser) ATCGAAGGGCCAAATGGAGA′] 877 NM_024426.4(WT1): c.1351T > C 7490 WT1 [′CTCTGTCCATTTAGGTGTGAAACCAYTCCAG (p.Phe451Leu) TGTAAAACTTGTCAGCGAAA′] 878 NM_024426.4(WT1): c.1378T > C 7490 WT1 [′CCAGTGTAAAACTTGTCAGCGAAAGYTCTCC (p.Phe460Leu) CGGTCCGACCACCTGAAGAC′] 879 NM_005957.4(MTHFR): c.968T > 4524 MTHFR [′GTGCCAGGCCTCCACTTCTACACCCYCAACC C (p.Leu323Pro) GCGAGATGGCTACCACAGAG′] 880 NM_016247.3(IMPG2): c.370T > 50939 IMPG2 [′TCACGCCCAGGAAGTCGATCCCAAARAGTC C (p.Phe124Leu) CTGAAGGCTTCCCAGACAGCT′] 881 NM_002225.3(IVD): c.134T > C 3712 IVD [′CCCGTGGACGATGCAATCAATGGGCYAAGC (p.Leu45Pro) GAGGAGCAGAGGCAGGTGAGG′] 882 NM_001127328.2(ACADM): c. 34 ACADM [′CAGTTAGCTACTGATGCTGTGCAGAYACTTG 1136T > C (p.Ile379Thr) GAGGCAATGGATTTAATACA′] 883 NM_001127328.2(ACADM): c. 34 ACADM [′TTAGGAATTAAACATGGGCCAGCGAYGTTC 742T > C (p.Cys248Arg) AGATACTAGAGGAATTGTCTT′] 884 NM_000016.5(ACADM): c.199T> 34 ACADM [′GGAAATCATCCCAGTGGCTGCAGAAYATGA C (p.Tyr67His) TAAAACTGGTGAAGTAGGTAT′] 885 NM_000155.3(GALT): c.221T > C 2592 GALT [′CCCCGCCATGACCCTCTCAACCCTCBGTGTC (p.Leu74Pro) CTGGGGCCATCCGAGCCAAC′] 886 NM_000155.3(GALT): c.512T > C 2592 GALT [′CTCCGTATCCCTATCTGATAGATCTYTGAAA (p.Phe171Ser) ACAAAGGTGCCATGATGGGC′] 887 NM_000155.3(GALT): c.580T > C 2592 GALT [′CCCTTGACAGGTATGGGCCAGCAGTYTCCTG (p.Phe194Leu) CCAGATATTGCCCAGCGTGA′] 888 NM_000250.1(MPO): c.752T > C 4353 MPO [′ACTCCGGACCAGGAGCGCTCACTCAYGTTC (p.Met251Thr) ATGCAATGGGGCCAGCTGTTG′] 889 NM_020247.4(ADCK3): c.1398 + 56997 ADCK3 [′CTCAGCCAGGAGATTCGGAACGAGGYTTGT 2T > C CTGTGCCAGCAGACAGGTGGG′] 890 NM_000229.1(LCAT): c.508T > C 3931 LCAT [′GACTGTGCGCGCCGCCCCCTATGACYGGCG (p.Trp170Arg) GCTGGAGCCCGGTGAGTGTCT′] 891 NM_000229.1(LCAT): c.698T > C 3931 LCAT [′CGCTTTATTGATGGCTTCATCTCTCYTGGGG (p.Leu233Pro) CTCCCTGGGGTGGCTCCATC′] 892 NM_000229.1(LCAT): c.524- 3931 LCAT [′CAGGTGCCCCAGACCCCAGCTGCCCYGACC 22T > C CCTTCCACCCGCTGCAGGCCA′] 893 NM_000403.3(GALE): c.548T > C 2582 GALE [′CCACAGACTTGGAACGCAGTGCTGCYGCGC (p.Leu183Pro) TATTTCAACCCCACAGGTGCC′] 894 NM_000527.4(LDLR): c.694 + 2T > 3949 LDLR [′ACAAATCTGACGAGGAAAACTGCGGYATGG C GCGGGGCCAGGGTGGGGGCGG′] 895 NM_000375.2(UROS): c.217T > C 7390 UROS [′CAGAGCAGTGGAAGCAGCAGAGTTAYGTTT (p.Cys73Arg) GGAGCAAAACAATAAAACTGA] 896 NM_000375.2(UROS): c.-26 − 7390 UROS [′GCTAACATGCTCTTTCTTGGCCTTAYCAGTG 177T > C ACAGGGGTCTTCAGAAAGAA′] 897 NM_000372.4(TYR): c.265T > C 7299 TYR [′GCCTTCCGTCTTTTATAATAGGACCYGCCAG (p.Cys89Arg) TGCTCTGGCAACTTCATGGG′] 898 NM_000017.3(ACADS): c.529T > 35 ACADS [′CACCGCCCGGGCCGAGGGCGACTCABGGGT C (p.Trp177Arg) TCTGAATGGAACCAAAGCCTG′] 899 NM_000053.3(ATP7B): c.3443T > 540 ATP7B [′GCGCCTCAGCCACTCACGGTTTCCARTCAGC C (p.Ile1148Thr) ACAGAGAAGGTCTGGGGGAC′] 900 NM_000053.3(ATP7B): c.2123T > 540 ATP7B [′GCCTTCACTGTCCTTGTCTTTCAGCYCCTCG C (pLeu708Pro) GTGGGTGGTACTTCTACGTT′] 901 NM_000520.4(HEXA): c.1453T > 3073 HEXA [′AGCAGGGGCTGTTGCCGAAAGGCTGYGGAG C (p.Trp485Arg) CAACAAGTTGACATCTGACCT′] 902 NM_000520.4(HEXA): c.632T > C 3073 HEXA [′TGGCATCTGGTAGATGATCCTTCCTYCCCAT (p.Phe211Ser) ATGAGAGCTTCACTTTTCCA′] 903 NM_000520.4(HEXA): c.538T > C 3073 HEXA [′CTTGCTGTTGGATACATCTCGCCATYACCTG (p.Tyr180His) CCACTCTCTAGCATCCTGGA′] 904 NM_016335.4(PRODH): c.1322T > 5625 PRODH [′CTGGGCCAGGTATGCGCCCCGCACCRGCTTG C (p.Leu441Pro) GCCCCAAAACACCAGCCCTC′] 905 NM_000152.3(GAA): c.953T > C 2548 GAA [′GTGTTCCTGCTAAACAGCAATGCCAYGGGT (p.Met318Thr) AAGCTGCCCGCCGCCCAGCGC′] 906 NM_012213.2(MLYCD): c.119T > 23417 MLYCD [′GCGGCCGGCGCCCTGGAGCGGGCCAYGGAC C (p.Met40Thr) GAGCTGCTGCGCCGCGCGGTG′] 907 NM_012464.4(TLL1): c.713T > C 7092 TLL1 [′AAGAACTGTGATAAATTTGGGATTGYTGTTC (p.Val238Ala) ATGAATTGGGTCATGTGATA′] 908 NM_000112.3(SLC26A2): c.- 1836 SLC26A2 [′CCTGCAGCGGCCCGGACCCGAGAGGYGAGA 26 + 2T > C AGAGGGAAGCGGACCAGGGAA′] 909 NM_138691.2(TMC1): c.1543T > 117531 TMC1 [′CCCTGCAGATGTACCTCGAGGACCTYGCTGG C (p.Cys515Arg) GAAACAATGGTGGGACAGGT′] 910 NM_138694.3(PKHD1): c.10658T > 5314 PKHD1 [′GTTGTCCTACAAGGAGAGGAGCCCAYTGAA C (p.Ile3553Thr) ATACGCTCAGGTGTTTCCATT′] 911 NM_001034116.1(EIF2B4): c.1393T > 8890 EIF2B4 [′TGCAGATGACCCTGATGATCTGCAAYGTAA C (p.Cys465Arg) GCGGGGAGAACATGTTGCGCT′] 912 NM_001034116.1(EIF2B4): c.1465T > 8890 EIF2B4 [′ATCCCTACGGTTGTTGAATCTAGTCYATGAT C (p.Tyr489His) GTGACTCCCCCAGAGCTTGT′] 913 NM_018960.4(GNMT): c.149T > 27232 GNMT [′ACCGCCGAGTACAAGGCATGGCTGCYTGGG C (p.Leu50Pro) CTGCTGCGCCAGCACGGCTGC′] 914 NM_181457.3(PAX3): c.268T > C 5077 PAX3 [′CTGCGTCTCCAAGATCCTGTGCAGGYACCAG (p.Tyr90His) GAGACTGGCTCCATACGTCC′] 915 NM_024301.4(FKRP): c.899T > C 79147 FKRP [′GAGACCACGCGCTGCTTCGGAACCGYGGTG (p.Val300Ala) GGCGACACGCCCGCCTACCTC′] 916 NM_021020.3(LZTS1): c.85T > C 11178 LZTS1 [′TTCGCAGTACAAGCTGCGCAAGTCCYCCCAC (p.Ser29Pro) CTCAAGAAGCTCAACCGGTA′] 917 NM_005857.4(ZMPSTE24): c.1018T > 10269 ZMPSTE24 [′TGTACTAGGCCATGAACTGGGGCACYGGAA  C (p.Trp340Arg) GTTGGGACATACAGTCAAAAA′] 918 NM_017838.3(NHP2): c.415T > C 55651 NHP2 [GCCCCATGAGGAGTACCAGGAGGCTYACGA (p.Tyr139His) TGAGTGCCTGGAGGAGGTGCA′] 919 NM_000157.3(GBA): c.1448T > C 2629 GBA [′GGGATGCATCAGTGCCACTGCGTCCVGGTC (p.Leu483Pro) GTTCTTCTGACTGGCAACCAG′] 920 NM_001005741.2(GBA): c.751T > 2629 GBA [′CATCTACCACCAGACCTGGGCCAGAYACTTT C (p.Tyr251His) GTGAAGTAAGGGATCAGCAA′] 921 NM_001243133.1(NLRP3): c.1718T > 114548 NLRP3 [′AAATTCGAAAAGGGGTATTTGATTTYTGTTG C (p.Phe573Ser) TACGTTTCCTCTTTGGCCTG′] 922 NM_001243133.1(NLRP3): c.926T > 114548 NLRP3 [′GGCTTCGATGAGCTGCAAGGTGCCTYTGAC C (p.Phe309Ser) GAGCACATAGGACCGCTCTGC′] 923 NM_001243133.1(NLRP3): c.1058T > 114548 NLRP3 [′CTCATCACCACGAGACCTGTGGCCCYGGAG C (p.Leu353Pro) AAACTGCAGCACTTGCTGGAC′] 924 NM_057176.2(BSND): c.35T > C 7809 BSND [′GAGAAGACCTTCCGGATCGGCTTCAYTGTGC (p.Ile12Thr) TGGGGCTTTTCCTGCTGGCC′] 925 NM_001195794.1(CLRN1): c.488T > 7401 CLRN1 [′TCTGTTGCAGGCTCCTGTGGCTGTCYTGTCA C (p.Leu163Pro) TGATATTGTTTGCCTCTGAA′] 926 NM_175073.2(APTX): c.668T > C 54840 APTX [′GCTGTGGCCAGGGAACACCTTGAACYCCTT (p.Leu223Pro) AAGCATATGCACACTGTGGGG′] 927 NM_020365.4(E1F2B3): c.1037T > 8891 EIF2B3 [′CCACCAGTCCATTCGTCAGCCCAGAYTGTCA C (p.Ile346Thr) GCAAACACCTGGTAAGTGCT′] 928 NM_031433.3(MFRP): c.545T > C −1 — [′ATCCAGGTGGCCACAGACCATGCAAYACAG (p.Ile182Thr) CTCAAGATCGAAGCCCTCAGC′] 929 NM_016180.4(SLC45A2): c.1082T > 51151 SLC45A2 [′AGTGCACACAACTCCACAGAGTTTCYCATCT C (p.Leu361Pro) ACGAAAGAGGAGTCGAGGTT′] 930 NM_006005.3(VVFS1): c.2486T > 7466 WFS1 [′AGCCTCATCGAGTTCAGCACCATCCYGGAG C (p.Leu829Pro) GGCCGCCTGGGCAGCAAGTGG′] 931 NM_024960.4(PANK2): c.178T > 80025 PANK2 [′GATCAAAGGAATTTTATACATTGACYCAGTC C (p.Ser60Pro) GGATTCAATGGACGGTCACA′] 932 NM_024960.4(PANK2): c.437T > 80025 PANK2 [′ACCACTTTTGAAGAAGCTCTTGAAAYGGCAT C (p.Met146Thr) CTCGTGGAGATAGCACCAAA′] 933 NM_020427.2(SLURP1): c.43T > 57152 SLURP1 [′TGTGCAGCTGCTGCTCGTGGCAGCCYGGAG C (p.Trpl5Arg) CATGGGCTGTGGTGAGTGGGC′] 934 NM_020427.2(SLURP1): c.229T > 57152 SLURP1 [′GGTGACCCGCTCCTGCTCCAGCTCCYGTGTG C (p.Cys77Arg) GCCACCGACCCCGACAGCAT′] 935 NM_021830.4(C10orf2): c.1142T > 56652 C10orf2 [′CTTCGGGAGGAGGTGCTAGGAGAACBGTCA C (p.Leu381Pro) AATGTGGAGCAAGCAGCTGGC′] 936 NM_001033855.2(DCLRE1C): c. 64421 DCLRE1C [′CCGGACTCTGGGATCGGCGGCGCTAYGAGT 2T > C (p.Met1Thr) TCTTTCGAGGGGCAGATGGCC′] 937 NM_153741.1(DPM3): c.254T > C 54344 DPM3 [′CAGATACAGGAGGCCCGAGCCGACTYAGCC (p.Leu85Ser) CGCAGGGGGCTGCGCTTCTGA′] 938 NM_000441.1(SLC26A4): c.707T > 5172 SLC26A4 [′GCCTTCCAAGTGCTGGTCTCACAGCYAAAG C (p.Leu236Pro) ATTGTCCTCAATGTTTCAACC′] 939 NM_000441.1(SLC26A4): c.1588T > 5172 SLC26A4 [′TGGAAGCATCCCTAGCACAGATATCYACAA C (p.Tyr530His) AAGTACCAAGAATTACAAAAA′] 940 NM_000441.1(SLC26A4): c.- −1 — [CTTTCCCTTCGACCAAGGTGTCTGTYGCTCC 103T > C GTAAATAAAACGTCCCACTG′] 941 NM_000441.1(SLC26A4): c.1003T > 5172 SLC26A4 [′ATTTTTCACTTAAAAACTCACTAGGHTTTTG C (p.Phe335Leu) CCTCCTGAACTTCCACCTGT′] 942 NM_022458.3(LMBR1): c.423 + 64327 LMBR1 [′AACAAAGATTTTTTTAATATGTTTCYATCCT 4842T > C GTGTCACAGTTTGAAATTGT′] 943 NM_022458.3(LMBR1): c.423 + 64327 LMBR1 [′AAAGCTGAGCAACATGACAGCACAAYAGAG 4808T > C GAGGAACAAAGATTTTTTTAA′] 944 NM_145693.2(LPIN1): c.1441 + 23175 LPIN1 [′GCGACCACCGGGAGATCACGAAAGGYACCG 2T > C CGGGCCTCGCGCGGGCGCCCT′] 945 NM_022124.5(CDH23): c.5663T > 64072 CDH23 [′AGTGGCTGCAATGCACGCCTCACCTYCAAC C (p.Phe1888Ser) ATCACTGCGGGCAACCGCGAG′] 946 NM_153212.2(GJB4): c.409T > C 127534 GJB4 [′GTGGACGTACTTGCTGAGCCTCATCYTCAAG (p.Phe137Leu) GCCGCCGTGGATGCTGGCTT′] 947 NM_021044.2(DHH): c.2T > C 50846 DHH [′TTTTGGCCGAGGTCCGCTGTATCCAYGGCTC (p.Met1Thr) TCCTGACCAATCTACTGCCC′] 948 NM_021044.2(DHH): c.485T > C 50846 DHH [′CGCGACCGCAACAAGTATGGGTTGCYGGCG (p.Leu162Pro) CGCCTCGCAGTGGAAGCCGGC′] 949 NM_020638.2(FGF23): c.287T > 8074 FGF23 [′GTGATGAGCAGAAGATACCTCTGCAYGGAT C (p.Met96Thr) TTCAGAGGCAACATTTTTGGA′] 950 NM_022041.3(GAN): c.1268T > C 8139 GAN [′TGCTATGCAGCTATGAAAAAGAAAAYCTAC (p.Ile423Thr) GCCATGGGTGGAGGCTCCTAC′] 951 NM_015665.5(AAAS): c.787T > C 8086 AAAS [′CAGTGGGGGGCGGCTGCTCTCAGCTYCACC (p.Ser263Pro) CGTGGATGCTGCTATCCGGGT′] 952 NM_021615.4(CHST6): c.827T > 4166 CHST6 [′TACCGCCTGGTGCGCTTCGAGGACCYGGCG C (p.Leu276Pro) CGGGAGCCGCTGGCAGAAATC′] 953 NM_000368.4(TSC1): c.539T > C 7248 TSC1 [′GTGGCGGAAGTCTATCTCGTCCATCYCCATG (p.Leu180Pro) CCAGTGTGTACGCACTCTTT′] 954 NM_020661.2(AICDA): c.238T > 57379 AICDA [′CCCTGGCCGCTGCTACCGCGTCACCYGGTTC C (p.Trp80Arg) ACCTCCTGGAGCCCCTGCTA′] 955 NM_020661.2(AICDA): c.317T > 57379 AICDA [′CTGCGAGGGAACCCCAACCTCAGTCYGAGG C (p.Leu106Pro) ATCTTCACCGCGCGCCTCTAC′] 956 NM_020661.2(AICDA): c.452T > 57379 AICDA [′GATTATTTTTACTGCTGGAATACTTYTGTAG C (p.Phe151Ser) AAAACCACGAAAGAACTTTC′] 957 NM_020632.2(ATP6V0A4): c. 50617 ATP6V0A4 [′ATCATTCTGCAATTTATCCCTGAGAYGATTT 1739T > C (p.Met580Thr) TTATCCTGTGTCTGTTTGGA′] 958 NM_054027.4(ANKH): c.143T > 56172 ANKH [′GCTGTCAAGGAGGATGCAGTCGAGAYGCTG C (p.Met48Thr) GCCAGCTACGGGCTGGCGTAC′] 959 NM_054027.4(ANKH): c.1015T > 56172 ANKH [′TGTTTGATGTCTTTCTCCCCAGCTCYGTTTCG C (p.Cys339Arg) TGATGTTTTGGACACCCAA′] 960 NM_054027.4(ANKH): c.1172T > −1 — [′GTGAGGGCGCATCTCACCGGGTGGCYGATG C (p.Leu391Pro) ACACTGAAGAAAACCTTCGTC′] 961 NM_016373.3(WWOX): c.872T > 51741 WWOX [′ACAAAAAACGACTATTGGGCGATGCYGGCT C (p.Leu291Pro) TATAACAGGTCCAAGCTCTGC′] 962 NM_021102.3(SPINT2): c.337 + 10653 SPINT2 [′CAGCGGATTCCTCTGTCCCAAGTGGYAGGTT 2T > C CTTAAAGAGACCCGCGATGG′] 963 NM_014588.5(VSX1): c.50T > C 30813 VSX1 [′GACGGGCGCACTAGCAGCAGGGCGCYGGTG (p.Leu17Pro) CCTGGCGGTTCCCCTAGGGGC′] 964 NM_006946.2(SPTBN2): c.758T > 6712 SPTBN2 [′GAAAAGGAACTGGGACTTACCAAGCYGCTG C (p.Leu253Pro) GATCCCGAAGGTGGGGCCAGA′] 965 NM_012452.2(TNFRSF13B): c. 23495 TNFRSF13B [′GCACCCTAAGCAATGTGCATACTTCYGTGAG 310T > C (p.Cys104Arg) AACAAGCTCAGGAGCCCAGT′] 966 NM_170784.2(MKKS): c.830T > 8195 MKKS [′GAAAATGCAGTCTTGGACCAGCTGCYTAAC C (p.Leu277Pro) CTAGGAAGGCAGCTAATCAGT′] 967 NM_015717.4(CD207): c.790T > 50489 CD207 [′AATGGCGTGTCATCCACCCAGGACCRGTCCC C (p.Trp264Arg) CTTCCATCCCTGCTTTAGTC′] 968 NM_153717.2(EVC): c.919T > C 2121 EVC [′TGTGGAAAAGAAGGAGAGAGAATACYCTGA (p.Ser307Pro) ACAGCTAATCGATAATGTGCG′] 969 NM_014384.2(ACAD8): c.455T > 27034 ACAD8 [′AAATTTTGCCCACCGCTCTGTACCAYGGAGA C (p.Met152Thr) AGTTTGCTTCCTACTGCCTC′] 970 NM_004924.4(ACTN4): c.784T > 81 ACTN4 [′CGAGAAGGCCATAATGACCTATGTGYCCAG C (p.Ser262Pro) CTTCTACCATGCCTTTTCAGG′] 971 NM_005097.3(LGI1): c.136T > C 9211 LGI1 [′AGCGAAGCCAAAATGCCCTGCCGTGYGTAC (p.Cys46Arg) TTGTACCAAAGATAATGCTTT′] 972 NM_005097.3(LGI1): c.695T > C 9211 LGI1 [′GCAGAATTTGCAAAGTCTCAAGACCYGCCTT (p.Leu232Pro) ATCAATCATTGTCCATAGAC′] 973 NM_006329.3(FBLN5): c.679T > 10516 FBLN5 [′GCAAACCTGCGTCAACACCTACGGCYCTTTC C (p.Ser227Pro) ATCTGCCGCTGTGACCCAGG′] 974 NM_006329.3(FBLN5): c.506T > 10516 FBLN5 [′TTGCTTGCATTTCTGTTTCCAGACAYTGATG C (p.Ile169Thr) AATGTCGCTATGGTTACTGC′] 975 NM_013339.3(ALG6): c.1432T > 29929 ALG6 [′GTTTTCTGTATTGGTGTGTTTTGTAYCTTGCT C (p.Ser478Pro) TGAACTTCCTGTTCTTCTT′] 976 NM_006899.3(IDH3B): c.395T > 3420 IDH3B [′GAGCTAGCCTCCTATGATATGCGGCYGAGG C (p.Leu132Pro) TAGGTGGTCTGGGTGGGGTGA′] 977 NM_014363.5(SACS): c.5836T > 26278 SACS [′AATGGATTATACTTACTATGCAGTAYGGCCC C (p.Trp1946Arg) GATCCTGATTTAGTTCATGA′] 978 NM_014363.5(SACS): c.9742T > 26278 SACS [′AAGTGAGTCTTGGCTTAAGAATGCAYGGCA C (p.Trp3248Arg) TTTTATTAGTGAATCTGTAAG′] 979 NM_014363.5(SACS): c.3161T > 26278 SACS [′CAGATGGTATCAGCTGGTGAACTCTYTGACC C (p.Phe1054Ser) CTGATATAGAAGTACTAAAG′] 980 NM_014324.5(AMACR): c.154T > −1 — [′GAGCCGCTTGGGCCGGGGCAAGCGCYCGCT C (p.Ser52Pro) AGTGCTGGACCTGAAGCAGCC′] 981 NM_014324.5(AMACR): c.320T > −1 — [′AATCCAAGGCTTATTTATGCCAGGCYGAGTG C (p.Leu107Pro) GATTTGGCCAGTCAGGAAGC′] 982 NM_001040108.1(MLH3): c.3826T > 27030 MLH3 [′GACAGAGGAACAAAGGAGACTCTTAYGGTC C (p.T1p1276Arg) AGTACCACCATGAGAATGTGA′] 983 NM_014336.4(A1PL1): c.715T > C 23746 AIPL1 [′TACTCTGATCCTCAACTACTGCCAGYGCCTG (p.Cys239Arg) CTGAAGAAGGAGGAGTACTA′] 984 NM_001001486.1(ATP2C1): c. 27032 ATP2C1 [′CTCTCATTTGCTTTAGCCAGTCGTCYGGGAT 1751T > C (p.Leu584Pro) TGTATTCCAAAACTTCCCAG′] 985 NM_007194.3(CHEK2): c.470T > 11200 CHEK2 [′GAAGTGGGTCCTAAAAACTCTTACAYTGCAT C (p.Ile157Thr) ACATAGAAGATCACAGTGGC′] 986 NM_007255.2(B4GALT7): c.617T > 11285 B4GALT7 [′ACCTATGTCGGCGGCATCCTGCTGCYCTCCA C (pLeu206Pro) AGCAGCACTACCGGCTGGTG′] 987 NM_000030.2(AGXT): c.613T > C 189 AGXT [′ATCTACAGGCATCGACATCCTGTACYCGGGC (p.Ser205Pro) TCCCAGAAGGCCCTGAACGC′] 988 NM_000030.2(AGXT): c.731T > C 189 AGXT [′AAGCCCTTCTCCTTCTACCTGGACAYCAAGT (p.Ile244Thr) GGCTGGCCAACTTCTGGGGC′] 989 NM_201253.2(CRB1): c.3122T > 23418 CRB1 [′ACATGGCACGAAGTGACCCTTTCCAYGACA C (p.Met1041Thr) GACCCACTGTCCCAGACCTCC′] 990 NM_201253.2(CRB1): c.3541T > 23418 CRB1 [′ACACTGTGAACTCAACATCGATGAAYGCTTT C (p.Cys1181Arg) TCAAACCCCTGTATCCATGG′] 991 NM_005094.3(SLC27A4): c.739T > 10999 SLC27A4 [′AGATAAACTGTTCTACATCTACACAYCCGGC C (p.Ser247Pro) ACCACAGGGCTGCCCAAGGC′] 992 NM_032551.4(KISS1R): c.443T > 84634 KISS1R [′CGCTGGTACGTGACGGTGTTCCCGTYGCGCG C (p.Leu148Ser) CCCTGCACCGCCGCACGCCC′] 993 NM_032551.4(KISS1R): c.305T > 84634 KISS1R [′TGCTGCGTCCCCTTCACGGCCCTGCYGTACC C (p.Leu102Pro) CGCTGCCCGGCTGGGTGCTG′] 994 NM_014270.4(SLC7A9): c.131T > 11136 SLC7A9 [′ATCTCCATCATCGTGGGCACCATCAYTGGCT C (p.Ile44Thr) CTGGGATCTTCGTTTCCCCC′] 995 NM_003332.3(TYROBP): c.2T > 7305 TYROBP [′TGGTGTCCAGCAGCATCCGGCTTCAYGGGG C (p.Met1Thr) GGACTTGAACCCTGCAGCAGG′] 996 NM_000843.3(GRM6): c.1214T > −1 — [′CAGGAGGGCAAGGTGCAGTTTGTGAYTGAT C (p.Ile405Thr) GCGGTGTACGCCATTGCCCAC′] 997 NM_022336.3(EDAR): c.259T > C 10913 EDAR [′GTTTTCCAAAGGAGGCTACCAGATAYGCAG (p.Cys87Arg) GCGTCACAAAGACTGTGAGGG′] 998 NM_003835.3(RGS9): c.895T > C 8787 RGS9 [′CCCAACCAAGATGCGAGTGGAACGAYGGGC (p.Trp299Arg) CTTCAACTTCAGCGAATTGAT′] 999 NM_004870.3(MPDU1): c.356T > 9526 MPDU1 [′CTCCAGACGATCACCATCTGCTTCCYGGTCA C (p.Leu119Pro) TGCACTACAGAGGACAGACT′] 1000 NM_004870.3(MPDU1): c.2T > C 9526 MPDU1 [′ACTGGCGGAAGCTAGCTTTGCAATAYGGCG (p.Met1Thr) GCCGAGGCGGACGGACCGCTT′] 1001 NM_004870.3(MPDU1): c.221T > 9526 MPDU1 [′CTGGGAGCCAAGAGTGCTGAAGGGTYGAGT C (p.Leu74Ser) CTCCAGTCTGTAATGCTGGAG′] 1002 NM_000334.4(SCN4A): c.2078T > 6329 SCN4A [′ACGCTGAACATGCTCATCAAGATCAYTGGC C (p.Ile693Thr) AATTCAGTGGGGGCGCTGGGT′] 1003 NM_006580.3(CLDN16): c.500T > 10686 CLDN16 [′GCTGGGTTTGGATTTCTCACCCTGCYCCTTG C (p.Leu167Pro) GTCTTGACTGCGTGAAATTC′] 1004 NM_006580.3(CLDN16): c.434T > 10686 CLDN16 [′ATATGCCCTGGTCTTCCAGTGAAGCYGGTGG C (p.Leu145Pro) TAACTCGAGCGTTGATGATT′] 1005 NM_003907.2(EIF2B5): c.1882T > 8893 EIF2B5 [′CTTTCTTCCATAGCTGCTAAAGGCCYGGAGC C (p.Trp628Arg) CCTGTTTTTAGGAACTACAT′] 1006 NM_183235.2(RAB27A): c.389T > 5873 RAB27A [′TATTGTGAAAACCCAGATATAGTGCYGTGTG C (p.Leu130Pro) GAAACAAGAGTGATCTGGAG′] 1007 NM_001128227.2(GNE): c.2228T > 10020 GNE [′CCCGCCCTGCTGGGTGCTGCCAGCAYGGTTC C (p.Met743Thr) TGGACTACACAACACGCAGG′] 1008 NM_004273.4(CHST3): c.776T > 9469 CHST3 [′CGCTGCGGCCCCCTCAACGTGACGCYGGCC C (p.Leu259Pro) GCAGAGGCCTGCCGCCGCAAG′] 1009 NM_004273.4(CHST3): c.920T > 9469 CHST3 [′CTGGTGCGCGACCCCCGGGCCGTGCYGGCC C (p.Leu307Pro) TCGCGCATGGTGGCCTTCGCC′] 1010 NM_004273.4(CHST3): c.857T > 9469 CHST3 [′CGGCAGCTGGAGTTCCTGCAGCCGCYGGCC C (p.Leu286Pro) GAGGACCCCCGCCTGGACCTG′] 1011 NM_172201.1(KCNE2): c.161T > 9992 KCNE2 [′TTCTACTATGTCATCCTGTACCTCAYGGTGA C (p.Met54Thr) TGATTGGAATGTTCTCTTTC′] 1012 NM_172201.1(KCNE2): c.170T > 9992 KCNE2 [′GTCATCCTGTACCTCATGGTGATGAYTGGAA C (p.Ile57Thr) TGTTCTCTTTCATCATCGTG′] 1013 NM_172201.1(KCNE2): c.178T > 9992 KCNE2 [′GTACCTCATGGTGATGATTGGAATGHTCTCT C (p.Phe60Leu) TTCATCATCGTGGCCATCCT′] 1014 NM_001139.2(ALOX12B): c.1277T> 242 ALOX12B [′TCATCCTCCTTGCTCTCCCCACAGCYCCTCA > C (p.Leu426Pro) TCCCCCATACCCGATACACC′] 1015 NM_003640.3(IKBKAP): c.2204 + 8518 IKBKAP [′ATTCGGAAGTGGTTGGACAAGTAAGYGCCA 6T > C TTGTACTGTTTGCGACTAGTT′] 1016 NM_005413.3(SIX3): c.749T > C 6496 SIX3 [′GCCACCGGCCTCACTCCCACACAAGYAGGC (p.Val250Ala) AACTGGTTTAAGAACCGGCGG′] 1017 NM_004820.3(CYP7B1): c.647T > 9420 CYP7B1 [HITATTAGTGAGCTAAGAGATGATTYTTTAA C (p.Phe216Ser) AATTTGATGACAAGTTTGCA′] 1018 NM_194248.2(OTOF): c.3032T > 9381 OTOF ′CTGTGTCCCACCTGGGACCAGATGCYGGTGT C (p.Leu1011Pro) TCGACAACCTGGAGCTCTAT] 1019 NM_194248.2(OTOF): c.1544T > 9381 OTOF [′GACGTGGCCATCGGCACCCACTTCAYTGACC C (p.Ile515Thr) TGCGCAAGATTTCTAATGAC′] 1020 NM_004737.4(LARGE): c.1483T > 9215 LARGE [′GATGCTGGAGGCCATCTGCAAGCACYGGGA C (p.Trp495Arg) GGGGCCCATCAGCCTGGCCCT′] 1021 NM_002420.5(TRPM1): c.296T > 4308 TRPM1 [′GAGGCCTCCATGCACAGATATTAAGRGCTTG C (p.Leu99Pro) GGGAGTTCCAGCTGCCAATC′] 1022 NM_004700.3(KCNQ4): c.842T > 9132 KCNQ4 [′ACCTGTTTGTGTCTCCAGATTACATYGACAA C (p.Leu281Ser) CCATCGGCTATGGTGACAAG′] 1023 NM_003839.3(TNFRSF11A): c. 8792 TNFRSF11 [′ACTGAACCACCTTTTCCCCCACAGCYGTACC 523T > C (p.Cys175Arg) TTCCTTGGAAAGAGAGTAGA′] 1024 NM_030761.4(WNT4): c.35T > C 54361 WNT4 [′CGCTCGTGCCTGCGTTCGCTGCGCCYCCTCG (p.Leu12Pro) TCTTCGCCGTCTTCTCAGCC′] 1025 NM_000050.4(ASS1): c.535T > C 445 ASS1 [′CATCCCGGTCACTCCCAAGAACCCGYGGAG (p.Trp179Arg) CATGGATGAGAACCTCATGCA′] 1026 NM_002977.3(SCN9A): c.2543T > −1 — [′ACATTGAACATGCTGATTAAGATCAYTGGTA C (p.Ile848Thr) ACTCAGTAGGGGCTCTAGGT′] 1027 NM_002977.3(SCN9A): c.4382T > −1 — [′TTTCTTTACCTTGGAGGTCAAGACAYCTTTA C (p.Ile1461Thr) TGACAGAAGAACAGAAGAAA′] 1028 NM_002977.3(SCN9A): c.647T > 6335 SCN9A [′GGCAATGTTTCAGCTCTTCGAACTTYCAGAG C (p.Phe216Ser) TATTGAGAGCTTTGAAAACT′] 1029 NM_003880.3(WISP3): c.232T > 8838 WISP3 [′AGATGGCTGTGGATGCTGTAAAATCYGTGC C (p.Cys78Arg) CAAGCAACCAGGGGAAATCTG′] 1030 NM_003880.3(WISP3): c.1000T > 8838 WISP3 [′TAAATGGAAGATGCTGTGGATTACAYCTTGT C (p.Ser334Pro) GTGTGTCAGAGAAACTGCAG′] 1031 NM_001457.3(FLNB): c.703T > C 2317 FLNB [′CGAGCACTCAGTTATGACTTACCTGYCCCAG (p.Ser235Pro) TTCCCCAAAGCCAAGCTCAA′] 1032 NM_003060.3(SLC22A5): c.1051T > 6584 SLC22A5 [′GATTTGTTACTGACAAGGCCTAGGGNAAGTT - C (p.Trp351Arg) TTCACAGCCTAAAACACAGT′, 1036 ′TACAAGACCTAAGTTCCAAGAAGCARCAGTC CCACAACATTCTGGATCTGC′, ′GGTCACCATCATGTCCATAATGCTGYGGTAT GTAAAAGAGACCTGCCTGAG′, ′ATGCCTCAGACAAAATTCAAAGCCTRTGTCA TCAGAGAGTGAAAAGGATAT′, ′GGCTGTTGTGGGAAATATGGACTCTYGTGGG GAATCTCTCCAGATCTTAAG′] 1037 NM_000369.2(TSHR): c.1891T > 7253 TSHR [′TGCCAAGAGGATGGCTGTGTTGATCYTCACC C (p.Phe631Leu) GACTTCATATGCATGGCCCC′] 1038 NM_000369.2(TSHR): c.1358T > 7253 TSHR [′AAACTGAACGTCCCCCGCTTTCTCAYGTGCA C (p.Met453Thr) ACCTGGCCTTTGCGGATTTC′] 1039 NM_000369.2(TSHR): c.1526T > 7253 TSHR [′ACTGTCTTTGCAAGCGAGTTATCGGYGTATA C (p.Val509Ala) CGCTGACGGTCATCACCCTG′] 1040 NM_000369.2(TSHR): c.1798T > 7253 TSHR [′AGTTGCCTTCGTCATCGTCTGCTGCYGTTAT C (p.Cys600Arg) GTGAAGATCTACATCACAGT′] 1041 NM_000369.2(TSHR): c.1400T > 7253 TSHR [′GCGGATTTCTGCATGGGGATGTACCYGCTCC C (p.Leu467Pro) TCATCGCCTCTGTAGACCTC′] 1042 NM_001003722.1(GLE1): c.2051T > 2733 GLE1 [′CAGAAATGTTTGCAACACAAGGACAYTCCT C (p.Ile684Thr) GTCCCCAAGGGCTTTCTGACT′] 1043 NM_024009.2(GJB3): c.101T > C 2707 GJB3 [′TCCGTGGTGTTCGTCTTCCGGGTGCYGGTAT (p.Leu34Pro) ACGTGGTGGCTGCAGAGCGC′] 1044 NM_001080463.1(DYNC2H1): c. 79659 DYNC2H1 [′GATTTGCTCAGAGTAGCTGATACAAYTGTAG 3719T > C (p.Ile1240Thr) CCAAAGCTGCCGACCTTAAA′] 1045 NM_018129.3(PNPO): c.784T > C 55163 PNPO [CTGGCTCTATGAGAGACTTGCACCTYAACTC (p.Ter262Gln) TGGGACCTGCTGGCCCAGAG′] 1046 NM_003722.4(TP63): c.1033T > C 8626 TP63 [′CCGACGCTGCTTTGAGGCCCGGATCYGTGCT (p.Cys345Arg) TGCCCAGGAAGAGACAGGAA′] 1047 NM_003722.4(TP63): c.1646T > C 8626 TP63 [′CCTCCGTATCCCACAGATTGCAGCAYTGTCA (p.Ile549Thr) GGTGAGTCCACAGCATGTGC′] 1048 NM_003722.4(TP63): c.1738T > C 8626 TP63 [′CACCATCTATCAGATTGAGCATTACBCCATG (p.Ser580Pro) GATGTAAGTAACTGTTAGAC′] 1049 NM_004086.2(COCH): c.349T > C −1 — [′CATCCAGTCTCAAATGCTTTCTAGAYGGTCT (p.Trp117Arg) GCTTCTTTCACAGTAACTAG′] 1050 NM_004086.2(COCH): c.1535T > −1 — [′GCACCTCTGGATGACCTGAAAGATAYGGCT C (p.Met512Thr) TCTAAACCGAAGGAGTCTCAT′] 1051 NM_006412.3(AGPAT2): c.683T > 10555 AGPAT2 [′ACAGGAACAGTCACAGTGCAGGTGCYGGAA C (p.Leu228Pro) GCCATCCCCACCAGCGGCCTC′] 1052 NM_003659.3(AGPS): c.1406T > 8540 AGPS [′AATCAGCTAAGTGTAGCCACATTACYGTTTG C (p.Leu469Pro) AGGGGGATCGTGAGAAGGTT′] 1053 NM_004550.4(NDUFS2): c.1237T > 4720 NDUFS2 [′GGGAGAGTTTGGGGTGTACCTGGTGYCTGA C (p.Ser413Pro) TGGCAGCAGCCGCCCTTATCG′] 1054 NM_006892.3(DNMT3B): c.808T > 1789 DNMT3B [′CCAGTGGTTTGGCGATGGCAAGTTCYCCGA C (p.Ser270Pro) GGTGAGTCCGGGGAAGGGCAA′] 1055 NM_002538.3(OCLN): c.656T > C 100506658 OCLN [′CAAATATATGCCCTCTGCAACCAATYTTATA (p.Phe219Ser) CACCTGCAGCTACTGGACTC′] 1056 NM_001211.5(BUB1B): c.3035T > −1 — [′AATGATGAGGCCACAGTGTCTGTTCYTGGG C (p.Leu1012Pro) GAGCTTGCAGCAGAAATGAAT′] 1057 NM_016203.3(PRKAG2): c.1459T > 51422 PRKAG2 [′ACAGAATCTTGCTGCTGAGAAAACAYACAA C (p.Tyr487His) TAACCTAGATATCACGGTGAC′] 1058 NM_016203.3(PRKAG2): c.1642T > 51422 PRKAG2 [′TAGTATTGTGGGTATTATTTCCCTGYCGGAC C (p.Ser548Pro) ATTCTGCAAGCCCTGATCCT′] 1059 NM_001287.5(CLCN7): c.2297T > 1186 CLCN7 [′TTCAAGCTGTTCCGGGCCCTGGGCCYGCGGC C (p.Leu766Pro) ACCTGGTGGTGGTGGACAAC′] 1060 NM_004646.3(NPHS1): c.793T > 4868 NPHS1 [′GGCAGGACAGAGCTTGGAGCTGCCGYGCGT C (p.Cys265Arg) GGCCCGAGGGGGTAATCCCTT′] 1061 NM_003002.3(SDHD): c.416T > C 6392 SDHD [′CTTTCAGCTTTAACCTTTGCTGGGCYTTGCT (p.Leu139Pro) ATTTCAACTATCACGATGTG′] 1062 NM_001164277.1(SLC37A4): c. 2542 SLC37A4 [′TAATGGCCTGGCCCAGGGGCTGGGCYGGCC 352T > C (p.Trp118Arg) CCCATGTGGGAAGGTCCTGCG′] 1063 NM_002546.3(TNFRSF11B): c. 4982 TNFRSF11B [′GGAAGGGCGCTACCTTGAGATAGAGYTCTG 349T > C (p.Phe117Leu) CTTGAAACATAGGAGCTGCCC′] 1064 NM_005422.2(TECTA): c.5509T > 7007 TECTA [′GGGCGTGAGGATCAATGACAGACAGBGCAC C (p.Cys1837Arg) CGGCATCGAGGGGGAAGATTT′] 1065 NM_007262.4(PARK7): c.497T > 11315 PARK7 [′CCTGGGACCAGCTTCGAGTTTGCGCYTGCAA C (p.Leu166Pro) TTGTTGAAGCCCTGAATGGC′] 1066 NM_006009.3(TUBA1A): c.1190T > 7846 TUBA1A [′GCTCGCCTGGACCACAAGTTTGACCYGATGT C (p.Leu397Pro) ATGCCAAACGTGCCTTTGTT′] 1067 NM_002700.2(POU4F3): c.668T > 5459 POU4F3 [′CTCAAGATCCCCGGCGTGGGCTCGCYGAGC C (p.Leu223Pro) CAAAGCACCATCTGCAGGTTC′] 1068 NM_005025.4(SERPINI1): c.145T > 5274 SERPINI1 [′TGGTGAAGATGAAAATATTCTCTTCYCTCCA C (p.Ser49Pro) TTGAGTATTGCTCTTGCAAT′] 1069 NM_001040667.2(HSF4): c.341T > 3299 HSF4 [′TTCGTGCGCGGCCGCGAGCAGCTACYGGAG C (p.Leu114Pro) CGCGTGCGGCGCAAGGTGGGG′] 1070 NM_002942.4(ROBO2): c.2834T > 6092 ROBO2 [′AATAGCAACAGTGGCCCAAATGAGAYTGGA C (p.Ile945Thr) AATTTTGGCCGTGGAGGTAAG′] 1071 NM_000492.3(CFTR): c.3857T > 1080 CFTR [′ACTTTGCAACAGTGGAGGAAAGCCTYTGGA C (p.Phe1286Ser) GTGATACCACAGGTGAGCAAA′] 1072 NM_000492.3(CFTR): c.3763T > 1080 CFTR [′TGGATCAGGGAAGAGTACTTTGTTAYCAGCT C (p.Ser1255Pro) TTTTTGAGACTACTGAACAC′] 1073 NM_000492.3(CFTR): c.3194T > 1080 CFTR [′ACAAGCTTAAAAGGACTATGGACACBTCGT C (p.Leu1065Pro) GCCTTCGGACGGCAGCCTTAC′] 1074 NM_000492.3(CFTR): c.3469 − 1080 CFTR [′TGTCTGCCATTCTTAAAAACAAAAAYGTTGT 20T > C TATTTTTATTTCAGATGCGA′] 1075 NM_005603.4(ATP8B1): c.863T > 5205 ATP8B1 [′TTTTGGAGAAACACAAGTTTTCCTTYGGATG C (p.Leu288Ser) CTGATAAAATTTTGTTACGT′] 1076 NM_005603.4(ATP8B1): c.2097 + 5205 ATP8B1 [′GAGGAGATTGAAAAAGACTTAATTGYGAGT C TTTAGCCTTAATAACTTTTTC′] 1077 NM_005603.4(ATP8B1): c.1982T > 5205 ATP8B1 [′ACCCTATGCCTTTGCTACAAGGAAAYTGAA C (p.Ile661Thr) GAAAAAGAATTTACAGAATGG′] 1078 NM_005144.4(HR): c.-320T > C 55806 HR [′CGCAGCACGGAGTCTCGGCGTCCCAYGGCG CAACCTACGGCCTCGGCCCAG′] 1079 NM_003322.4(TULP1): c.1471T > 7287 TULP1 [′GGTCACCCAGGCCTCAGTCAAGAACYTCCA C (p.Phe491Leu) GATTGTCCACGCTGATGACCG′] 1080 NM_003322.4(TULP1): c.1145T > 7287 TULP1 [CTCCTGGGGAACCGCTTCACGGTCTYTGACA C (p.Phe382Ser) ACGGGCAGAACCCACAGCGT′] 1081 NM_000455.4(STK11): c.200T > 6794 STK11 [′TCTTACGGCAAGGTGAAGGAGGTGCYGGAC C (p.Leu67Pro) TCGGAGACGCTGTGCAGGAGG′] 1082 NM_002241.4(KCNJ10): c.418T > 3766 KCNJ10 [′TGGCTTCCGCTACATCAGTGAGGAAYGTCCA C (p.Cys140Arg) CTGGCCATTGTGCTTCTTAT′] 1083 NM_001172567.1(MYD88): c.317T > 4615 MYD88 [′TCTGTAGGCCGACTGCTCGAGCTGCYTACCA C (p.Leu106Pro) AGCTGGGCCGCGACGACGTG′] 1084 NM_000466.2(PEX1): c.1991T > 5189 PEX1 [′CCATCTGTTGTCCTGCTGGATGACCYTGACC C (p.Leu664Pro) TCATTGCTGGACTGCCTGCT′] 1085 NM_001303.3(COX10): c.2T > C 1352 COX10 [′GGAGCGGCCCCAGACTCGTAAATTAYGGCC (p.Met1Thr) GCATCTCCGCACACTCTCTCC′] 1086 NM_001300.5(KLF6): c.346T > C 1316 KLF6 [′TGTCAGCAGCGAATCCTCTGACAGCYCCGA (p.Ser116Pro) GGAACTTTCTCCCACGGCCAA′] 1087 NM_001300.5(KLF6): c.190T > C 1316 KLF6 [′CAAATTTGACAGCCAGGAAGATCTGYGGAC (p.Trp64Arg) CAAAATCATTCTGGCTCGGGA′] 1088 NM_001300.5(KLF6): c.506T > C 1316 KLF6 [′CTGTGGGGTTGCGTGCCCGGGGAGCYGCCC (p.Leu169Pro) TCGCCAGGGAAGGTGCGCAGC′] 1089 NM_000085.4(CLCNKB): c.1294T > 1188 CLCNKB [′CGGGTACTTCATGCCCATCTTTGTCYATGGT C (p.Tyr432His) GAGTCTGGGGTCCTGAGGTT′] 1090 NM_000214.2(JAG1): c.110T > C 182 JAG1 [TGTGGGGCCTCGGGTCAGTTCGAGTYGGAG (p.Leu37Ser) ATCCTGTCCATGCAGAACGTG′] 1091 NM_003865.2(HESX1): c.77T > C 8820 HESX1 [′ACTTGCTCCTTTTCAATTGAGAGAAYCTTAG (p.Ile26Thr) GACTGGACCAGAAGAAAGAC′] 1092 NM_003865.2(HESX1): c.357 + 8820 HESX1 [′TCATTATTGGGTGAAAAAACTTCCCDCCTGG 2T > C TTTTGAGTAAAAGCAGTTCT′] 1093 NM_002618.3(PEX13): c.977T > 5194 PEX13 [′CTTGATGGCCAAACAACAGGACTTAYACCT C (p.Ile326Thr) GCGAATTATGTCAAAATTCTT′] 1094 NM_000303.2(PMM2): c.395T > 5373 PMM2 [′AATGGGATGTTAAACGTGTCCCCTAYTGGA C (p.Ile132Thr) AGAAGCTGCAGCCAAGAAGAA′] 1095 NM_000303.2(PMM2): c.131T > 5373 PMM2 [′AGGCAGAAGATCAAAATCGGAGTGGYAGGC C (p.Val44Ala) GGATCGGACTTTGAGAAAGTG′] 1096 NM_000104.3(CYP1B1): c.2T > C 1545 CYP1B1 [′CGCCTTCTCCTCTCTGTCCCCAGCAYGGGCA (p.Met1Thr) CCAGCCTCAGCCCGAACGAC′] 1097 NM_032977.3(CASP10): c.440T > 843 CASP10 [′AAAGACTCGCTTCCCAAAACTGAAAYGGTG C (p.Met147Thr) AGTGGGTCATACAGAATGGGT′] 1098 NM_003999.2(OSMR): c.2072T > 9180 OSMR [′GGTTCAGAATGTTGCAAATACAAAAYTGAC C (p.Ile691Thr) AACCCGGAAGAAAAGGCATTG′] 1099 NM_000314.6(PTEN): c.370T > C 5728 PTEN [′TGACAATCATGTTGCAGCAATTCACBGTAAA (p.Cys124Arg) GCTGGAAAGGGACGAACTGG′] 1100 NM_000314.6(PTEN): c.209T > C 5728 PTEN [′AAAAACCATTACAAGATATACAATCYGTAA (p.Leu70Pro) GTATGTTTTCTTATTTGTATG′] 1101 NM_000314.6(PTEN): c.335T > C 5728 PTEN [′TTTTGTGAAGATCTTGACCAATGGCYAAGTG (p.Leu112Pro) AAGATGACAATCATGTTGCA′] 1102 NM_000314.6(PTEN): c.722T > C 5728 PTEN [′CGACGGGAAGACAAGTTCATGTACTYTGAG (p.Phe241Ser) TTCCCTCAGCCGTTACCTGTG′] 1103 NM_006949.3(STXBP2): c.626T > 6813 STXBP2 [′TTGGCCCACGCCGTCCTGGCCAAGCHGAAC C (p.Leu209Pro) GCCTTCAAGGCAGACACTCCC′] 1104 NM_000358.2(TGFBI): c.1619T > 7045 TGFBI [′AACCGGGAAGGAGTCTACACAGTCTYTGCT C (p.Phe540Ser) CCCACAAATGAAGCCTTCCGA′] 1105 NM_000350.2(ABCA4): c.1622T > 24 ABCA4 [′CAGCTCACCCAACGTGCCCTCTCTCYACTGG C (p.Leu541Pro) AGGAAAACATGTTCTGGGCC′] 1106 NM_000350.2(ABCA4): c.5819T > 24 ABCA4 [′GGAAATAAAACTGACATCTTAAGGCYACAT C (p.Leu1940Pro) GAACTAACCAAGGTAAGGGAA′] 1107 NM_000860.5(HPGD): c.577T > C 3248 HPGD [′CTTTGTTAACACAGCCATCCTTGAAYCAATT (p.Ser193Pro) GAAAAAGAAGAAAACATGGG′] 1108 NM_000223.3(KRT12): c.386T > 3859 KRT12 [′CTTTCTGGATCAGAAAAAGAAACTAYGCAA C (p.Met129Thr) AATCTTAATGATAGATTAGCT′] 1109 NM_000503.5(EYA1): c.1459T > 2138 EYA1 [′GTTGACACTGGCCCTGAAAGCACTCYCGCTC C (p.Ser487Pro) ATTCACTCCCGGTGAGGCTC′] 1110 NM_000261.1(MYOC): c.1309T > 4653 MYOC [′TGCCTTCATCATCTGTGGCACCTTGYACACC C (p.Tyr437His) GTCAGCAGCTACACCTCAGC′] 1111 NM_000261.1(MYOC): c.1297T > 4653 MYOC [′GTCAGTCGCCAATGCCTTCATCATCYGTGGC C (p.Cys433Arg) ACCTTGTACACCGTCAGCAG′] 1112 NM_130838.1(UBE3A): c.389T > 7337 UBE3A [′GATTATTCCCCTTTAATCCGTGTTAYTGGAA C (p.Ile130Thr) GAGTTTTTTCTAGTGCTGAG′] 1113 NM_000474.3(TWIST1): c.392T > 7291 TWIST1 [′TCGCTGAACGAGGCGTTCGCCGCGCYGCGG C (p.Leu131Pro) AAGATCATCCCCACGCTGCCC′] 1114 NM_000192.3(TBX5): c.161T > C 6910 TBX5 [′TCCTTCTTGCAGGGCATGGAGGGAAYCAAA (p.Ile54Thr) GTGTTTCTCCATGAAAGAGAA′] 1115 NM_002905.3(RDH5): c.841T > C −1 — [′GACTGCTCGACACCCCCGAACCCGCBACAG (p.Tyr281His) CCCAGGTTGGGATGCCAAGCT′] 1116 NM_001089.2(ABCA3): c.302T > 21 ABCA3 [′GTCACTGAGACAGTGCGCAGGGCACYTGTG C (p.Leu101Pro) ATCAACATGCGAGGTGAGACA′] 1117 NM_001089.2(ABCA3): c.4658T > 21 ABCA3 [′ATGGACCCCGTGGCCCGGCGCCTGCYTTGG C (p.Leu1553Pro) GACACCGTGGCACGAGCCCGA′] 1118 NM_001089.2(ABCA3): c.977T > 21 ABCA3 [′ATCGCCGCCTCCTTCATGACCCTGCYCTTCT C (p.Leu326Pro) GTGTCAAGGTGAGCATGGCG′] 1119 NM_005535.2(IL12RB1): c.592T > 3594 IL12RB1 [′CTGCCTCTCCCCAGAGTCCTGCCTCYGCCCC C (p.Cys198Arg) CTGGAGATGAATGTGGCCCA′] 1120 NM_005055.4(RAPSN): c.41T > C 5913 RAPSN [′ACCAAGCAGCAGATCGAGAAGGGGCYCCAG (p.Leu14Pro) CTGTACCAGTCCAACCAGACA′] 1121 NM_005055.4(RAPSN): c.848T > 5913 RAPSN [′ATCATGACCGAGATCGGAAACCGCCYGGGG C (p.Leu283Pro) CAGGTGCAGGCGCTGCTGGGT′] 1122 NM_005055.4(RAPSN): c.416T > 5913 RAPSN MATGCCTTCCTGGGCCTCAGCGTCTYCCAGA C (p.Phe139Ser) AGGCCCTGGAGAGCTTCGAG′] 1123 NM_005570.3(LMAN1): c.2T > C 3998 LMAN1 [′CTCCTCCGCGTTCCAGAATCCAAGAYGGCG (p.Met1Thr) GGATCCAGGCAAAGGGGTCTC′] 1124 NM_000430.3(PAFAH1B1): c.505T > 5048 PAFAH1B1 [CAGCGGCAAGCTTCTGGCTTCCTGTYCTGCA C (p.Ser169Pro) GATATGACCATTAAACTATG′] 1125 NM_000430.3(PAFAH1B1): c.92T > 5048 PAFAH1B1 [′GGCTATGAAGAGGCATATTCAGTTTYTAAA C (p.Phe31Ser) AAGGAAGCTGAATTAGATGTG′] 1126 NM_006261.4(PROP1): c.263T > 5626 PROP1 [′CAGTTGGAACAGCTGGAGTCAGCCTYTGGG C (p.Phe88Ser) AGGAACCAGTACCCCGACATC′] 1127 NM_175929.2(FGF14): c.449T > 2259 FGF14 [′GAACTTTTTACCCCTGAATGCAAGTYTAAAG C (p.Phe150Ser) AATCTGTTTTTGAAAATTAT′] 1128 NM_004970.2(IGFALS): c.1618T > 3483 IGFALS [′GTGGCTGGAGGGTAACCCCTGGGACYGTGG C (p.Cys540Arg) CTGCCCTCTCAAGGCGCTGCG′] 1129 NM_005379.3(MYO1A): c.2728T > 4640 MYO1A [′CCCTTGCCTGTTCCGTCCCTAGACTYCTTCTC C (p.Ser910Pro) GGATTCTCCTCCTGACCAA′] 1130 NM_006302.2(MOGS): c.1954T > 7841 MOGS [′GCACTGGGCCCCAGAGCTAGGAGTCYTTGC C (p.Phe652Leu) AGACTTTGGGAACCACACAAA′] 1131 NM_001009944.2(PKD1): c.2534T > 5310 PKD1 [′TACGTGCCCACCAACGGCTCAGCCTYGGTGC C (p.Leu845Ser) TCCAGGTGGACTCTGGTGCC′] 1132 NM_004329.2(BMPR1A): c.370T > 657 BMPR1A [′AGCCCAGCTACGCCGGACAATAGAAYGTTG C (p.Cys124Arg) TCGGACCAATTTATGTAACCA′] 1133 NM_004329.2(BMPR1A): c.1409T > 657 BMPR1A [′CCGAGTGATCCGTCATACGAAGATAYGCGT C (p.Met470Thr) GAGGTTGTGTGTGTCAAACGT′] 1134 NM_000452.2(SLC10A2): c.728T > 6555 SLC10A2 [′GTGGCGGGTTACTCCCTGGGGTTTCYTCTGG C (p.Leu243Pro) CTAGAATTGCTGGTCTACCC′] 1135 NM_030662.3(MAP2K2): c.400T > 5605 MAP2K2 [′CAACTCGCCGTACATCGTGGGCTTCYACGGG C (p.Tyr134His) GCCTTCTACAGTGACGGGGA′] 1136 NM_033337.2(CAV3): c.260T > C 859 CAV3 [′CTGCTGGGCGTCCCACTGGCCCTGCHCTGGG (p.Leu87Pro) GCTTCCTGTTCGCCTGCATC′] 1137 NM_000388.3(CASR): c.2417T > 846 CASR [′CCGGAGAACTTCAATGAAGCCAAGTHCATC C (p.Phe806Ser) ACCTTCAGCATGCTCATCTTC′] 1138 NM_000388.3(CASR): c.382T > C 846 CASR [′AATTGATTCTTTGAACCTTGATGAGYTCTGC (p.Phe128Leu) AACTGCTCAGAGCACATTCC′] 1139 NM_000388.3(CASR): c.1835T > 846 CASR [GAGTTTCTGTCGTGGACGGAGCCCTYTGGGA C (p.Phe612Ser) TCGCACTCACCCTCTTTGCC′] 1140 NM_000388.3(CASR): c.2641T > 846 CASR [′GCGTTGCAGCACCGCAGCTCACGCTYTCAA C (p.Phe881Leu) GGTGGCTGCCCGGGCCACGCT′] 1141 NM_000388.3(CASR): c.374T > C 846 CASR [′CAAAACAAAATTGATTCTTTGAACCYTGATG (p.Leu125Pro) AGTTCTGCAACTGCTCAGAG′] 1142 NM_000388.3(CASR): c.2362T > 846 CASR [′CACCTGCCTGCTGGCTGCCATCTGCYTCTTC C (p.Phe788Leu) TTTGCCTTCAAGTCCCGGAA′] 1143 NM_000388.3(CASR): c.38T > C 846 CASR [′AGCTGCTGCTGGGTCCTCTTGGCACYCACCT (p.Leu13Pro) GGCACACCTCTGCCTACGGG′] 1144 NM_030653.3(DDX11): c.2271 + 1663 DDX11 [′CTGGCATATTCCAGGTGCATCCAGGYGCGG 2T > C GCGTCATGCTGGGCTTGGGTC′] 1145 NM_001001557.2(GDF6): c.866T > 392255 GDF6 [′TTCACCAGATCCCAGCGCAAGAACCYGTTC C (p.Leu289Pro) GCAGAGATGCGCGAGCAGCTG′] 1146 NM_000557.4(GDF5): c.1322T > −1 — [′TGCGAGTTCCCATTGCGCTCCCACCYGGAGC C (p.Leu441Pro) CCACGAATCATGCAGTCATC′] 1147 NM_000392.4(ABCC2): c.1967 + 1244 ABCC2 [′TGATTCGGAAGCCACAGTCCGAGAGYGAGT 2T > C TGCCTTCTTTCCATCCTAATG′] 1148 NM_000396.3(CTSK): c.926T > C 1513 CTSK [′AACTGGGGAAACAAAGGATATATCCYCATG (p.Leu309Pro) GCTCGAAATAAGAACAACGCC′] 1149 NM_000304.3(PMP22): c.47T > C 5376 PMP22 [′ATCATCGTCCTCCACGTCGCGGTGCYGGTGC (p.Leu16Pro) TGCTGTTCGTCTCCACGATC′] 1150 NM_000304.3(PMP22): c.82T > C 5376 PMP22 [′ATATCTATCTGATTCTCTCTAGCAAYGGATC (p.Trp28Arg) GTGGGCAATGGACACGCAAC′] 1151 NM_001453.2(FOXC1): c.335T > 2296 FOXC1 [′ATCTACCAGTTCATCATGGACCGCTHCCCCT C (p.Phe112Ser) TCTACCGGGACAACAAGCAG′] 1152 NM_001451.2(FOXF1): c.1138T > 2294 FOXF1 [′CCAAGACATCAAGCCTTGCGTGATGYGAGG C (p.Ter380Arg) CTGCCGCCGCAGGCCCTCCTG′] 1153 NM_006432.3(NPC2): c.199T > C 10577 NPC2 [′CTCTTTTTTTCTCTTAGATATTCAGYCTAAAA (p.Ser67Pro) GCAGCAAGGCCGTGGTGCA′] 1154 NM_001127221.1(CACNA1A): c. 773 CACNA1A [′CTGAATGTGTTCTTGGCCATCGCTGYGGACA 2141T > C (p.Val714Ala) ATCTGGCCAACGCCCAGGAG′] 1155 NM_001127221.1(CACNA1A): c. 773 CACNA1A [′GGGTACCGCATGGAGATGTCCATTTYCTACG 4469T > C (p.Phe1490Ser) TCGTCTACTTTGTGGTGTTC′] 1156 NM_001127221.1(CACNA1A): c. 773 CACNA1A [′ATGCTCTTCTTCATCTATGCCATCAYTGGGA 5126T > C (p.Ile1709Thr) TGCAGGTGAGTGTCGTGTCC′] 1157 NM_000901.4(NR3C2): c.2771T > 4306 NR3C2 [′CAGAGCTGGCAGAGGTTCTACCAACYGACC C (p.Leu924Pro) AAGCTGCTGGACTCCATGCAT′] 1158 NM_000901.4(NR3C2): c.2936T > 4306 NR3C2 [′GTGGAGTCGGGGAACGCCAAGCCGCYCTAC C (p.Leu979Pro) TTCCACCGGAAGTGACTGCCC′] 1159 NM_000339.2(SLC12A3): c.2576T > 6559 SLC12A3 [′CTCACCCTCCTCATTCCCTATCTCCYTGGCC C (p.Leu859Pro) GCAAGAGGAGGTGGAGCAAA′] 1160 NM_000339.2(SLC12A3): c.1261T > 6559 SLC12A3 [′CTGGGGTGCCTGCGAGGGGCTGGCCYGCAG C (p.Cys421Arg) CTATGGCTGGAACTTCACCGA′] 1161 NM_000339.2(SLC12A3): c.1868T > 6559 SLC12A3 [′TCGGTACAGGCTGGCTCCTACAACCYGGCCC C (p.Leu623Pro) TCAGCTACTCGGTGGGCCTC′] 1162 NM_000163.4(GHR): c.341T > C 2690 GHR [′TCTGCTGGGGAAAACAGCTGTTACTYTAATT (p.Phe114Ser) CATCGTTTACCTCCATCTGG′] 1163 NM_000163.4(GHR): c.512T > C 2690 GHR [′AGTTTAACTGGGATTCATGCAGATAYCCAA (p.Ile171Thr) GTGAGATGGGAAGCACCACGC′] 1164 NM_000525.3(KCNJ11): c.440T > 3767 KCNJ11 [′ACTGAGGAGTGCCCACTGGCCATCCYGATC C (p.Leu147Pro) CTCATCGTGCAGAACATCGTG′] 1165 NM_000525.3(KCNJ11): c.124T > 3767 KCNJ11 [′CCGCTTTGTGTCCAAGAAAGGCAACYGCAA C (p.Cys42Arg) CGTGGCCCACAAGAACATCCG′] 1166 NM_001122764.1(PPDX): c.35T > 5498 PPDX [′ACCGTGGTCGTGCTGGGCGGAGGCAYCAGC C (p.Ile12Thr) GGCTTGGCCGCCAGTTACCAC′] 1167 NM_000182.4(HADHA): c.1025T > 3030 HADHA [′AAAGAATCAAAGGCCTTGATGGGACYCTAC C (p.Leu342Pro) CATGGTCAGGTCCTGTGCAAG′] 1168 NM_003476.4(CSRP3): c.131T > 8048 CSRP3 [′CTGGCAGTGGCCTGCAGGAAGGCTCYTGAC C (p.Leu44Pro) AGCACGACAGTCGCGGCTCAT′] 1169 NM_001204.6(BMPR2): c.367T > 659 BMPR2 [′TTTCTGCTGTTGTAGCACAGATTTAHGTAAT C (p.Cys123Arg) GTCAACTTTACTGAGAATTT′] 1170 NM_000336.2(SCNN1B): c.1858T > 6338 SCNN1B [′CATCCCAGGCACCCCGCCCCCCAACYATGA C (p.Tyr620His) CTCCCTGCGTCTGCAGCCGCT′] 1171 NM_002181.3(IHH): c.569T > C 3549 IHH [′TCAAAGGCCCACGTGCATTGCTCCGYCAAGT (p.Val190Ala) CCGGTGAGCCGCCGCCGGGG′] 1172 NM_000193.3(SHH): c.349T > C 6469 SHH [′TTTGGCCATCTCGGTGATGAACCAGBGGCCA (p.Trp117Arg) GGAGTGAAACTGCGGGTGAC′] 1173 NM_000193.3(SHH): c.995T > C 6469 SHH [′GACCGCCGGCTCCTGCCCGCCGCTGYGCAC (p.Val332Ala) AGCGTGACCCTAAGCGAGGAG′] 1174 NM_000179.2(MSH6): c.2633T > 2956 MSH6 [′AAAATTATAGGGATCATGGAAGAAGBTGCT C (p.Val878Ala) GATGGTTTTAAGTCTAAAATC′] 1175 NM_000098.2(CPT2): c.1342T > 1376 CPT2 [′AACCCTCACTATTGACTGCGTCCAGYTTCAG C (p.Phe448Leu) AGAGGAGGCAAAGAATTCCT′] 1176 NM_007315.3(STAT1): c.2117T > 6772 STAT1 [′GGAACTGGATATATCAAGACTGAGTYGATT C (p.Leu706Ser) TCTGTGTCTGAAGTGTAAGTG′] 1177 NM_007315.3(STAT1): c.1799T > 6772 STAT1 [′AAGGACCAGCAGCCGGGGACCTTCCYGCTG C (p.Leu600Pro) CGGTTCAGTGAGAGCTCCCGG′] 1178 NM_024420.2(PLA2G4A): c.331T > 5321 PLA2G4A [′AGGGACAGCAACATTTACTGTATCTYCTATG C (p.Ser111Pro) AAGGTGGGAGAAAAGAAAGA′] 1179 NM_000352.4(ABCC8): c.394T > 6833 ABCC8 [′CTATCACAACATCGAGACTTCCAACBTCCCC C (p.Phe132Leu) AAGCTGCTAATTGGTAGGTG′] 1180 NM_033163.3(FGF8): c.118T > C 2253 FGF8 [′GCTGGGCAGGGAGCTCGCTTCCCTGYTCCGG (p.Phe40Leu) GCTGGCCGGGAGCCCCAGGG′] 1181 NM_153767.3(KCNJ1): c.1013T > 3758 KCNJ1 [′AACATCTTTCTCATTATAAAGGCACRTGGCA C (p.Met338Thr) CAGTGAGGGGTCTCCACTTC′] 1182 NM_000095.2(COMP): c.982T > 1311 COMP [′AACCCACCCTGTCATCCAGGACAACYGCCC C (p.Cys328Arg) GCTGGTGCGGAACCCAGACCA′] 1183 NM_000095.2(COMP): c.1042T > 1311 COMP [′GGACGAGGACAAGTGGGGCGATGCGYGCGA C (p.Cys348Arg) CAACTGCCGGTCCCAGAAGAA′] 1184 NM_002047.2(GARS): c.548T > C 2617 GARS [′ATCCTGGAGATCGATTGCACCATGCYCACCC (p.Leu183Pro) CTGAGCCAGTTTTAAAGTGA′] 1185 NM_000435.2(NOTCH3): c.1363T > 4854 NOTCH3 [′CCTCGACCGCATAGGCCAGTTCACCYGTATC C (p.Cys455Arg) TGTATGGCAGGTGGGTGGTG′] 1186 NM_001166107.1(HMGCS2): c. 3158 HMGCS2 [′CTGCTACGGTGGTACTGCCTCCCTCYTCAAT 520T > C (p.Phe174Leu) GCTGCCAACTGGATGGAGTC′] 1187 NM_001038.5(SCNN1A): c.1477 6337 SCNN1A [′CCAGCTCTCTGCTGGTTACTCACGAYGGCCC C (p.Trp493Arg) TCGGTGACATCCCAGGTAGA′] 1188 NM_000161.2(GCH1): c.662T > C 2643 GCH1 [′ATGGTAATGCGAGGTGTACAGAAAAYGAAC (p.Met221Thr) AGCAAAACTGTGACCAGCACA′] 1189 NM_000059.3(BRCA2): c.7529T > 675 BRCA2 [′CGCGTCTTTCCACAGCCAGGCAGTCYGTATC C (p.Leu2510Pro) TTGCAAAAACATCCACTCTG′] 1190 NM_000180.3(GUCY2D): c.1694T > 3000 GUCY2D [′GGAGACAGGGTTTGGCTGAAGAAATYCCCA C (p.Phe565Ser) GGGGATCAGCACATAGCTATC′] 1191 NM_000180.3(GUCY2D): c.2846T > 3000 GUCY2D [′AATGGGCAGCGACACGCGGCAGAGAYCGCC C (p.Ile949Thr) AACATGTCACTGGACATCCTC′] 1192 NM_198056.2(SCN5A): c.3963 + 6331 SCN5A [′CTGTCACGATTTGAGGGCATGAGGGYAAGA 2T > C GAGGTGGCTGCCTTCCCACCA′] 1193 NM_000335.4(SCN5A): c.5380T > 6331 SCN5A [′GAGTGAGGACGACTTCGATATGTTCYATGA C (p.Tyr1794His) GATCTGGGAGAAATTTGACCC′] 1194 NM_000023.2(SGCA): c.524T > C 6442 SGCA [′CCCGGAGAGCTTCAGCTGCTCAACGYCACCT (p.Val175Ala) CTGCCTTGGACCGTGGGGGC′] 1195 NM_002427.3(MMP13): c.224T > 4322 MMP13 [′AGGCTCCGAGAAATGCAGTCTTTCTYCGGCT C (p.Phe75Ser) TAGAGGTGACTGGCAAACTT′] 1196 NM_002427.3(MMP13): c.221T > 4322 MMP13 [′GAGAGGCTCCGAGAAATGCAGTCTTYCTTC C (p.Phe74Ser) GGCTTAGAGGTGACTGGCAAA′] 1197 NM_002427.3(MMP13): c.272T > 4322 MMP13 [′CTTGACGATAACACCTTAGATGTCAYGAAA C (p.Met91Thr) AAGCCAAGATGCGGGGTTCCT′] 1198 NM_004525.2(LRP2): c.7564T > 4036 LRP2 [′CTCCTTTTGTATGACTAGGTACCTGYACTGG C (p.Tyr2522His) GCTGACTGGGATACACATGC′] 1199 NM_000211.4(ITGB2): c.446T > 3689 ITGB2 [′GATGACCTCAGGAATGTCAAGAAGCYAGGT C (p.Leu149Pro) GGCGACCTGCTCCGGGCCCTC′] 1200 NM_000211.4(ITGB2): c.412T > 3689 ITGB2 [′GTACTATCTGATGGACCTCTCCTACYCCATG C (p.Ser138Pro) CTTGATGACCTCAGGAATGT′] 1201 NM_005502.3(ABCA1): c.4429T > 19 ABCA1 [′CAAAATCAAGAAGATGCTGCCTGTGYGTCC C (p.Cys1477Arg) CCCAGGGGCAGGGGGGCTGCC′] 1202 NM_005502.3(ABCA1): c.6026T > 19 ABCA1 [′TTGACTGGGAGAGAACACGTGGAGTYCTTT C (p.Phe2009Ser) GCCCTTTTGAGAGGAGTCCCA′] 1203 NM_130439.3(MXI1): c.552 + 4601 MXI1 [′AACAAAGCCAAAGCACACATCAAGGYGAGA 2T > C ATTTTTACTTTCAGATTTGCA′] 1204 m.5874T > C 4579 MT-TY [′GTCTTTAGATTTACAGTCCAATGCTYCACTC AGCCATTTTACCTCACCCCC′] 1205 m.7512T > C 4574 MT-TS1 [′CCAACCCCATGGCCTCCATGACTTTYTCAAA AAGGTATTAGAAAAACCATT′] 1206 m.7510T > C 4574 MT-TS1 [′AGCCAACCCCATGGCCTCCATGACTYTTTCA AAAAGGTATTAGAAAAACCA′] 1207 m.7511T > C 4574 MT-TS1 [′GCCAACCCCATGGCCTCCATGACTTYTTCAA AAAGGTATTAGAAAAACCAT′] 1208 m.616T > C 4558 MT-TF [′CCTCCTCAAAGCAATACACTGAAAABGTTTA GACGGGCTCACATCACCCCA′] 1209 m.4409T > C 4569 MT-TM [′TATCACACCCCATCCTAAAGTAAGGYCAGCT AAATAAGCTATCGGGCCCAT′] 1210 m.8356T > C 4566 MT-TK [′AAAGATTAAGAGAACCAACACCTCTYTACA GTGAAATGCCCCAACTAAATA′] 1211 m.12297T > C 4568 MT-TL2 [′AAAGGATAACAGCTATCCATTGGTCYTAGG CCCCAAAAATTTTGGTGCAAC′] 1212 m.3271T > C 4567 MT-TL1 [′CCGGTAATCGCATAAAACTTAAAACYTTAC AGTCAGAGGTTCAATTCCTCT′] 1213 m.3250T > C 4567 MT-TL1 [′GGTTTGTTAAGATGGCAGAGCCCGGYAATC GCATAAAACTTAAAACTTTAC′] 1214 m.3290T > C 4567 MT-TL1 [′TAAAACTTTACAGTCAGAGGTTCAAYTCCTC TTCTTAACAACATACCCATG′] 1215 m.4290T > C 4565 MT-TI [′AAATATGTCTGATAAAAGAGTTACTYTGATA GAGTAAATAATAGGAGCTTA′] 1216 m.4291T > C 4565 MT-TI [′AATATGTCTGATAAAAGAGTTACTTYGATAG AGTAAATAATAGGAGCTTAA′] 1217 m.9997T > C 4563 MT-TG [′ATCTATTGATGAGGGTCTTACTCTTYTAGTA TAAATAGTACCGTTAACTTC′] 1218 m.10010T > C 4563 MT-TG [′GGTCTTACTCTTTTAGTATAAATAGYACCGT TAACTTCCAATTAACTAGTT′] 1219 m.4336T > C 4572 MT-TQ [′GCTTAAACCCCCTTATTTCTAGGACYATGAG AATCGAACCCATCCCTGAGA′] 1220 m.14709T > C 4556 MT-TE [′CGGACTACAACCACGACCAATGATAYGAAA AACCATCGTTGTATTTCAACT′] 1221 m.14674T > C 4556 MT-TE [′TCAACAGAAACAAAGCATACATCATBATTCT CGCACGGACTACAACCACGA′] 1222 m.5692T > C 4570 MT-TN [′GGGACTTAAACCCACAAACACTTAGYTAAC AGCTAAGCACCCTAATCAACT′] 1223 m.5728T > C 4570 MT-TN [′GCACCCTAATCAACTGGCTTCAATCYACTTC TCCCGCCGCCGGGAAAAAAG′] 1224 m.2991T > C 4550 MT-RNR2 [′TCAACAATAGGGTTTACGACCTCGAYGTTGG ATCAGGACATCCCGATGGTG′] 1225 m.1291T > C 4549 MT-RNR1 [′ATCTTCAGCAAACCCTGATGAAGGCYACAA AGTAAGCGCAAGTACCCACGT′] 1226 m.1095T > C 4549 MT-RNR1 [′CTGGGATTAGATACCCCACTATGCTYAGCCC TAAACCTCAACAGTTAAATC′] 1227 m.8528T > C −1 — [′TTATAACAAACCCTGAGAACCAAAAYGAAC GAAAATCTGTTCGCTTCATTC′] 1228 m.8993T > C 4508 MT-ATP6 [′AGCCTACTCATTCAACCAATAGCCCBGGCCG TACGCCTAACCGCTAACATT′] 1229 m.9101T > C 4508 MT-ATP6 [′ATTAACCTTCCCTCTACACTTATCAYCTTCA CAATTCTAATTCTACTGACT′] 1230 m.9176T > C 4508 MT-ATP6 [′ATCCAAGCCTACGTTTTCACACTTCBAGTAA GCCTCTACCTGCACGACAAC′] 1231 m.8851T > C 4508 MT-ATP6 [′AAACCTAGCCATGGCCATCCCCTTAYGAGC GGGCACAGTGATTATAGGCTT′] 1232 m.9185T > C 4508 MT-ATP6 [′TACGTTTTCACACTTCTAGTAAGCCYCTACC TGCACGACAACACATAATGA′] 1233 m.7587T > C 4513 MT-CO2 [′ATAGGCTAAATCCTATATATCTTAAYGGCAC ATGCAGCGCAAGTAGGTCTA′] 1234 m.6742T > C 4512 MT-CO1 [′GGTATGGTCTGAGCTATGATATCAAYTGGCT TCCTAGGGTTTATCGTGTGA′] 1235 m.6721T > C 4512 MT-CO1 [′AAAGAACCATTTGGATACATAGGTAYGGTC TGAGCTATGATATCAATTGGC′] 1236 m.7275T > C 4512 MT-CO1 [′CATGAAACATCCTATCATCTGTAGGYTCATT CATTTCTCTAACAGCAGTAA′] 1237 m.15572T > C 4519 MT-CYB [′CATCAAGCCCGAATGATATTTCCTAYTCGCC TACACAATTCTCCGATCCGT′] 1238 m.15197T > C 4519 MT-CYB [′GGCCACAGTAATTACAAACTTACTAYCCGCC ATCCCATACATTGGGACAGA′] 1239 m.14849T > C 4519 MT-CYB [′CATCTCCGCATGATGAAACTTCGGCYCACTC CTTGGCGCCTGCCTGATCCT′] 1240 NC_012920.1: m.14484T > C 4541 MT-ND6 [′GCTGTAGTATATCCAAAGACAACCAYCATTC CCCCTAAATAAATTAAAAAA′] 1241 m.14487T > C 4541 MT-ND6 [′GTAGTATATCCAAAGACAACCATCAYTCCCC CTAAATAAATTAAAAAAACT′] 1242 m.12706T > C 4540 MT-NDS [′ATCAGTTCTTCAAATATCTACTCATYTTCCT AATTACCATACTAATCTTAG′] 1243 m.10563T > C 4539 MT-ND4L [′CTCACACCTCATATCCTCCCTACTAYGCCTA GAAGGAATAATACTATCGCT′] 1244 m.10663T > C 4539 MT-ND4L [′ATATTGTGCCTATTGCCATACTAGTYTTTGC CGCCTGCGAAGCAGCGGTNG′] 1245 m.10191T > C 4537 MT-ND3 [′TTACGAGTGCGGCTTCGACCCTATAYCCCCC GCCCGCGTCCCTTTCTCCAT′] 1246 m.10158T > C 4537 MT-ND3 [′ACAACTCAACGGCTACATAGAAAAAYCCAC CCCTTACGAGTGCGGCTTCGA′] 1247 m.4681T > C 4536 MT-ND2 [′CAAGCAACCGCATCCATAATCCTTCYAATAG CTATCCTCTTCAACAATATA′] 1248 m.4160T > C 4535 MT-ND1 [′TACCCCCGATTCCGCTACGACCAACYCATAC ACCTCCTATGAAAAAACTTC′] 1249 m.3394T > C 4535 MT-ND1 [′GCTTACCGAACGAAAAATTCTAGGCYATAT ACAACTACGCAAAGGCCCCAA′] 1250 m.3308T > C 4535 MT-ND1 [′GGTTCAATTCCTCTTCTTAACAACABACCCA TGGCCAACCTCCTACTCCTC′] 1251 m.3949T > C 4535 MT-ND1 [′ACTAGTCTCAGGCTTCAACATCGAAYACGCC GCAGGCCCCTTCGCCCTATT′] 1252 NM_003140.2(SRY): c.326T > C 6736 SRY [′CTTACTGAAGCCGAAAAATGGCCATYCTTCC (p.Phe109Ser) AGGAGGCACAGAAATTACAG′] 1253 NM_003140.2(SRY): c.203T > C 6736 SRY [′GTGAAGCGACCCATGAACGCATTCAYCGTG (p.IIe68Thr) TGGTCTCGCGATCAGAGGCGC′] 1254 NM_021083.2(XK): c.880T > C 7504 XK [′ACTCTATACTGGTATCAACATGTTCNGCTGG (p.Cys294Arg) TCTGCTGTACAGCTGAAAAT′] 1255 NM_000354.5(SERPINA7): c. 6906 SERPINA7 [′CACCAGATGGAACAATACTATCACCYAGTG 740T > C (p.Leu247Pro) GATATGGAATTGAACTGCACA′] 1256 NM_000044.3(AR): c.2033T > C 367 AR [′TGTCAGCCCATCTTTCTGAATGTCCYGGAAG (p.Leu678Pro) CCATTGAGCCAGGTGTAGTG′] 1257 NM_000044.3(AR): c.2423T > C 367 AR [′ATCACCCCCCAGGAATTCCTGTGCAYGAAA (p.Met808Thr) GCACTGCTACTCTTCAGCATT′] 1258 NM_000044.3(AR): c.2596T > C 367 AR [′CTACCAGCTCACCAAGCTCCTGGACYCCGTG (p.Ser866Pro) CAGCCTGTAAGCAAACGATG′] 1259 NM_000451.3(SHOX): c.877T > C 6473 SHOX [′GAAGCACGCGGAGGCCCTGGGGCTCYGACC (p.Ter293Arg) CGCCGCGCAGCCCCCCGCGCG′] 1260 NM_000330.3(RS1): c.286T > C −1 — [′TTGAGCCGGGCCTTGTTTGCAGTCCRCGAAG (p.Trp96Arg) AATACCAGCCCACATACTGC′] 1261 NM_000330.3(RS1): c.38T > C 6247 RS1 [′ATAGAAGGCTTTTTGTTATTACTTCYCTTTG (p.Leu13Pro) GCTATGAAGGTATGTGCTAT′] 1262 NM_000330.3(RS1): c.667T > C −1 — [′CCGAGCTGAGGCAGGCATCAGGCACRCTTG (p.Cys223Arg) CTGACGCACTCCAGCAGCTCC′] 1263 NM_002764.3(PRPS1): c.344T > 5631 PRPS1 [′ATCTCAGCCAAGCTTGTTGCAAATAYGCTAT C (p.Met115Thr) CTGTAGCAGGTGCAGATCAT′] 1264 NM_002764.3(PRPS1): c.455T > 5631 PRPS1 [′AATTTGTATGCAGAGCCGGCTGTCCYAAAGT C (p.Leu152Pro) GGATAAGGGAGAATATCTCT′] 1265 NM_002764.3(PRPS1): c.869T > 5631 PRPS1 [′TTTCCTTTCTTGCCTTTCTAGGTGAYTGACAT C (p.Ile290Thr) CTCTATGATCCTTGCAGAA′] 1266 NM_000291.3(PGK1): c.263T > C 5230 PGK1 [′CCAGTTGCTGTAGAACTCAAATCTCYGCTGG (p.Leu88Pro) GCAAGTAAGTGCCAGGCTCT′] 1267 NM_000291.3(PGK1): c.946T > C 5230 PGK1 [′CACCTCTACCCCTCAGGGCTTGGACYGTGGT (p.Cys316Arg) CCTGAAAGCAGCAAGAAGTA′] 1268 NM_000291.3(PGK1): c.758T > C 5230 PGK1 [′CATCATTTTGGCTCCCCTGTGTAGAYTGGCA (p.Ile253Thr) CTTCTCTGTTTGATGAAGAG′] 1269 NM_001122606.1(LAMP2): c.961T > 3920 LAMP2 [′CATTGCAAATAACAATCTCAGCTACYGGGA C (p.Trp321Arg) TGCCCCCCTGGGAAGTTCTTA′] 1270 NM_000206.2(IL2RG): c.343T > 3561 IL2RG [′ATTCTCTGAAGAAATCACTTCTGGCYGTCAG C (p.Cys115Arg) TTGCAAAAAAAGGAGATCCA′] 1271 NM_000206.2(IL2RG): c.452T > 3561 IL2RG [′ACACAGATGCTAAAACTGCAGAATCYGGGT C (p.Leu151Pro) AATTTGGAAAGAAAGGGTCAA′] 1272 NM_000194.2(HPRT1): c.122T > 3251 HPRT1 [′GAAAGGGTGTTTATTCCTCATGGACYAATTA C (p.Leu41Pro) TGGACAGGTAAGTAAGATCT′] 1273 NM_000194.2(HPRT1): c.170T > 3251 HPRT1 [′CTTGCTCGAGATGTGATGAAGGAGAYGGGA C (p.Met57Thr) GGCCATCACATTGTAGCCCTC′] 1274 NM_000132.3(F8): c.6554T > C 2157 F8 [′ATTCGCAGCACTCTTCGCATGGAGTYGATGG (p.Leu2185Ser) GCTGTGATTTAAATAGTAAG′] 1275 NM_000132.3(F8): c.985T > C 2157 F8 [′GGACCTTGGACAGTTTCTACTGTTTYGTCAT (p.Cys329Arg) ATCTCTTCCCACCAACATGG′] 1276 NM_000132.3(F8): c.5372T > C 2157 F8 [′AGAGCAGAAGTTGAAGATAATATCAYGGTG (p.Met1791Thr) AGTTAAGGACAGTGGAATTAC′] 1277 NM_000132.3(F8): c.1754T > C 2157 F8 [′CCTTTCAATATATGTAATTAACAGAYAATGT (p.Ile585Thr) CAGACAAGAGGAATGTCATC′] 1278 NM_000132.3(F8): c.935T > C 2157 F8 [′TCCTTGGAAATCTCGCCAATAACTTYCCTTA (p.Phe312Ser) CTGCTCAAACACTCTTGATG′] 1279 NM_000132.3(F8): c.980T > C 2157 F8 [′TTGATGGACCTTGGACAGTTTCTACYGTTTT (p.Leu327Pro) GTCATATCTCTTCCCACCAA′] 1280 NM_000132.3(F8): c.1174T > C 2157 F8 [′CTCTCCTTCCTTTATCCAAATTCGCYCAGTTG (p.Ser392Pro) CCAAGAAGCATCCTAAAAC′] 1281 NM_000132.3(F8): c.1417T > C 2157 F8 [′ATCAGGAATCTTGGGACCTTTACTTYATGGG (p.Tyr473His) GAAGTTGGAGACACACTGTT′] 1282 NM_000132.3(F8): c.1481T > C 2157 F8 [′AATCAAGCAAGCAGACCATATAACAYCTAC (p.Ile494Thr) CCTCACGGAATCACTGATGTC′] 1283 NM_000132.3(F8): c.1729T > C 2157 F8 [′CCCTCTCCTCATCTGCTACAAAGAAYCTGTA (p.Ser577Pro) GATCAAAGAGGAAACCAGGT′] 1284 NM_000132.3(F8): c.1958T > C 2157 F8 [′CAGTTGTCAGTTTGTTTGCATGAGGYGGCAT (p.Val653Ala) ACTGGTACATTCTAAGCATT′] 1285 NM_000132.3(F8): c.2029T > C 2157 F8 [′TTCTGTCTTCTTCTCTGGATATACCYTCAAAC ACAAAATGGTCTATGAAGA′] 1286 NM_000132.3 (F8): c.6193T > C 2157 F8 [′GTTTCTTTACTTGGGCAAAGGACAGYGGGCC CCAAAGCTGGCCAGACTTCA′] 1287 NM_007325.4(GRIA3): c.2117T > 2892 GRIA3 [′GTGTACGAGAAAATGTGGTCTTACAYGAAA C (p.Met706Thr) TCAGCGGAGCCATCTGTGTTT′] 1288 NM_000402.4(G6PD): c.1058T > 2539 G6PD [′GAGGGCGAGGCCACCAAAGGGTACCYGGAC C (p.Leu353Pro) GACCCCACGGTGCCCCGCGGG′] 1289 NM_000402.4(G6PD): c.1054T > 2539 G6PD [′TGGAGAGGGCGAGGCCACCAAAGGGYACCT C (p.Tyr352His) GGACGACCCCACGGTGCCCCG′] 1290 NM_001097642.2(GJB1): c.397T > 2705 GJB1 [′GGTCCACATCTCAGGGACACTGTGGYGGAC C (p.Trp133Arg) CTATGTCATCAGCGTGGTGTT′] 1291 NM_000166.5(GJB1): c.407T > C 2705 GJB1 [′TCAGGGACACTGTGGTGGACCTATGYCATC (p.Val136Ala) AGCGTGGTGTTCCGGCTGTTG′] 1292 NM_000032.4(ALAS2): c.595T > 212 ALAS2 [′TGTGTCCGTCTGGTGTAGTAATGATYACCTG C (p.Tyr199His) GGCATGAGCCGACACCCTCA′] 1293 NM_020061.5(OPN1MW): c.607T > 5956 OPN1LW [′CTGGCCCCACGGCCTGAAGACTTCAYGCGG C (p.Cys203Arg) CCCAGACGTGTTCAGCGGCAG′] 1294 NM_000513.2(OPN1MW): c.607T > 2652 OPN1MW [′CTGGCCCCACGGCCTGAAGACTTCAYGCGG C (p.Cys203Arg) CCCAGACGTGTTCAGCGGCAG′] 1295 NM_000513.2(OPN1MW): c.529T > 2652 OPN1MW [′GGCCATCGTGGGCATTGCCTTCTCCYGGATC C (p.Trp177Arg) TGGGCTGCTGTGTGGACAGC′] 1296 NM_000273.2(GPR143): c.397T > 4935 GPR143 [′GCTGTTGTACAGTGCCTGCTTCTGGHGGCTG C (p.Trp133Arg) TTTTGCTATGCAGTGGATGC′] 1297 NM_000133.3(F9): c.52T > C 2158 F9 [′AGAATCACCAGGCCTCATCACCATCYGCCTT (p.Cys18Arg) TTAGGATATCTACTCAGTGC′] 1298 NM_000133.3(F9): c.1058T > C 2158 F9 [′TTCCTCAAATTTGGATCTGGCTATGYAAGTG (p.Val353Ala) GCTGGGGAAGAGTCTTCCAC′] 1299 NM_000133.3(F9): c.1144T > C 2158 F9 [′AGTTCCACTTGTTGACCGAGCCACAHGTCTT (p.Cys382Arg) CGATCTACAAAGTTCACCAT′] 1300 NM_000133.3(F9): c.1328T > C 2158 F9 [′TGTGCAATGAAAGGCAAATATGGAAYATAT (p.Ile443Thr) ACCAAGGTATCCCGGTATGTC′] 1301 NM_000133.3(F9): c.1357T > C 2158 F9 [′TACCAAGGTATCCCGGTATGTCAACYGGATT (p.Trp453Arg) AAGGAAAAAACAAAGCTCAC′] 1302 NM_000133.3(F9): c.82T > C 2158 F9 [′TTTAGGATATCTACTCAGTGCTGAAYGTACA (p.Cys28Arg) GGTTTGTTTCCTTTTTTAAA′] 1303 NM_000133.3(F9): c.277 + 2T > C 2158 F9 [′CTGAATTTTGGAAGCAGTATGTTGGYAAGC AATTCATTTTATCCTCTAGCT′] 1304 NM_000133.3(F9): c.1031T > C 2158 F9 [′ATTGCTGACAAGGAATACACGAACAYCTTC (p.Ile344Thr) CTCAAATTTGGATCTGGCTAT′] 1305 NM_000169.2(GLA): c.484T > C −1 — [′CATTGATGCCCAGACCTTTGCTGACYGGGGA (p.Trp162Arg) GTAGATCTGCTAAAATTTGA′] 1306 NM_000169.2(GLA): c.806T > C −1 — [′TTATTTCATTCTTTTTCTCAGTTAGYGATTGG (p.Val269Ala) CAACTTTGGCCTCAGCTGG′] 1307 NM_015107.2(PHF8): c.836T > C 23133 PHF8 [′CCAACAAATGCCAATCTGACTCTCTYTGAGT (p.Phe279Ser) GCTGGAGCAGTTCCTCTAAT′] 1308 NM_033290.3 (MID1): c.1877T > 4281 MID1 [′TATGATGCTTTGAACTCCATCCACCYCTACA C (p.Leu626Pro) CCTTCGACGTCGCATTTGCG′] 1309 NM_033290.3 (MID1): c.884T > C 4281 MID1 [′TTCAAGGTGATGAGGCTTCGCAAACYGGCT (p.Leu295Pro) CAGCAGATTGCAAACTGCAAA′] 1310 NM_000444.5(PHEX): c.755T > C 5251 PHEX [′CAGTATCGGGATGCCCTTTACAAGTYCATGG (p.Phe252Ser) TGGATACTGCCGTGCTTTTA′] 1311 NM_000444.5(PHEX): c.1664T > 5251 PHEX [′CTCATAGGATTTCCAGCAGGAGAGCYCCAG C (p.Leu555Pro) AAGCCTTTCTTTTGGGGAACA′] 1312 NM_001205019.1(GK): c.1525T > 2710 GK [′AGAAAGTGAAATTCGTTATTCTACAYGGAA C (p.Trp509Arg) GAAAGCTGTGATGAAGTCAAT′] 1313 NM_000475.4(NR0B1): c.890T > 190 NR0B1 [′AACTGCTGGGCGTCCCTGCTCATGCYTGAGC C (p.Leu297Pro) TGGCCCAGGACCGCTTGCAG′] 1314 NM_000531.5(OTC): c.332T > C 5009 OTC [′CTTCTGGGAGGACATCCTTGTTTTCYTACCA (p.Leu111Pro) CACAAGATATTCATTTGGGT′] 1315 NM_000531.5(OTC): c.134T > C 5009 OTC [′CTGAAGGGCCGTGACCTTCTCACTCYAAAA (p.Leu45Pro) AACTTTACCGGAGAAGAAATT′] 1316 NM_000531.5(OTC): c.717+2T > 5009 OTC [′TTGGCAGAGCAGTATGCCAAAGAGGYATGC C TCTTTACATGTAAAGCTATTA′] 1317 NM_001399.4(EDA): c.181T > C 1896 EDA [′CCTCCACCTGCTGACGTTGTGCTGCYACCTA (p.Tyr61His) GAGTTGCGCTCGGAGTTGCG′] 1318 NM_001015877.1(PHF6): c.2T > 84295 PHF6 [′CATTCTAAAGGCAATTTAAAAATCAYGTCA C (p.Met1Thr) AGCTCAGTTGAACAGAAAAAA′] 1319 NM_001128834.2(PLP1): c.487T > 5354 PLP1 [′CATCACCTATGCCCTGACCGTTGTGYGGCTC C (p.Trp163Arg) CTGGTGTTTGCCTGCTCTGC′] 1320 NM_001128834.2(PLP1): c.671T > 5354 PLP1 [′CCTGGCAAGGTTTGTGGCTCCAACCYTCTGT C (p.Leu224Pro) CCATCTGCAAAACAGCTGAG′] 1321 NM_001128834.2(PLP1): c.560T > 5354 PLP1 [′AACACCTGGACCACCTGCCAGTCTAYTGCCT C (p.Ile187Thr) TCCCCAGCAAGACCTCTGCC′] 1322 NM_001128834.2(PLP1): c.710T > 5354 PLP1 [ATTTTCCTGCAGTTCCAAATGACCTYCCACC C (p.Phe237Ser) TGTTTATTGCTGCATTTGTG′] 1323 NM_000116.4(TAZ): c.352T > C 6901 TAZ [′CTCCCACTTCTTCAGCTTGGGCAAGYGTGTG (p.Cys118Arg) CCTGTGTGCCGAGGTGAGCT′] 1324 NM_000377.2(WAS): c.244T > C 7454 WAS [′CTTCGTGAAGGATAACCCCCAGAAGYCCTA (p.Ser82Pro) CTTCATCCGCCTTTACGGCCT′] 1325 NM_000377.2(WAS): c.809T > C 7454 WAS [′AACCTCGACCCAGATCTGCGGAGTCYGTTCT (p.Leu270Pro) CCAGGGCAGGAATCAGCGAG′] 1326 NM_182680.1(AMELX): c.2T > C −1 — [′CTTACATTTCAGAACCATCAAGAAAYGGGG (p.Met1Thr) ACCTGGATTTTATTTGCCTGC′] 1327 NM_000074.2(CD40LG): c.464T > 959 CD40LG [′ACCATGAGCAACAACTTGGTAACCCYGGAA C (p.Leu155Pro) AATGGGAAACAGCTGACCGTT′] 1328 NM_139058.2(ARX): c.98T > C 170302 ARX [′TCCTCCTACTGCATCGACAGCATCCYGGGCC (p.Leu33Pro) GGAGGAGCCCGTGCAAAATG′] 1329 NM_001109878.1(TBX22): c.641T > 50945 TBX22 [′TGATCATTTCTCCTCCAGATCATTCYGCAAT C (p.Leu214Pro) CCATGCATAAGTACAAACCC′] 1330 NM_000061.2(BTK): c.2T > C 695 BTK [′GGTGAACTCCAGAAAGAAGAAGCTAYGGCC (p.Met1Thr) GCAGTGATTCTGGAGAGCATC′] 1331 NM_000061.2(BTK): c.1223T > C 695 BTK [′AAGGACCTGACCTTCTTGAAGGAGCYGGGG (p.Leu408Pro) ACTGGACAATTTGGGGTAGTG′] 1332 NM_000061.2(BTK): c.1516T > C 695 BTK [′GCTGCTAGAGATGTGCAAGGATGTCYGTGA (p.Cys506Arg) AGCCATGGAATACCTGGAGTC′] 1333 NM_000061.2(BTK): c.1625T > C 695 BTK [′GTTGTTAAAGTATCTGATTTCGGCCYGTCCA (p.Leu542Pro) GGTGAGTGTGGCTTTTTCAT′] 1334 NM_000061.2(BTK): c.1741T > C 695 BTK [′CAAGTTCAGCAGCAAATCTGACATTYGGGC (p.Trp581Arg) TTTTGGTAAGTGGATAAGATT′] 1335 NM_000061.2(BTK): c.1955T > C 695 BTK [′TTCAAAATTCTTCTGAGCAATATTCYAGATG (p.Leu652Pro) TCATGGATGAAGAATCCTGA′] 1336 NM_015884.3(MBTPS2): c.1424T > 51360 MBTPS2 [′GATGGACAATGGATTCTAAACTCTTYCTTGG C (p.Phe475Ser) ATGCCACCCTTACCTCAGTG′] 1337 NM_014009.3(FOXP3): c.970T > 50943 FOXP3 [′GATTCATCCCCACCCTCTGACAGAGYTCCTC C (p.Phe324Leu) CACAACATGGACTACTTCAA′] 1338 NM_014009.3(FOXP3): c.1099T > 50943 FOXP3 [′GACACTCAATGAGATCTACCACTGGYTCAC C (p.Phe367Leu) ACGCATGTTTGCCTTCTTCAG′] 1339 NM_022567.2(NYX): c.302T > C 60506 NYX [′CTGCGCCACAACAACCTGTCCTTCAYCACGC (p.Ile101Thr) CCGGCGCCTTCAAGGGCCTG′] 1340 NM_003639.4(IKBKG): c.1249T > 8517 EKBKG [′CACCCTGCAGATACATGTCATGGAGYGCATT C (p.Cys417Arg) GAGTAGGGCCGGCCAGTGCA′] 1341 NM_006579.2(EBP): c.53T > C 10682 EBP [′CCATACTGGCCTCAGCACCTAAGACYGGAC (p.Leu18Pro) AACTTTGTACCTAATGACCGC′] 1342 NM_003159.2(CDKL5): c.215T > 6792 CDKL5 [′CTTCGGACTCTCAAGCAGGAAAACAHTGTG C (p.Ile72Thr) GAGTTGAAGGAAGCATTTCGT′] 1343 NM_001011658.3(TRAPPC2): c. −1 — [′GCCTTAATTATTTCACATATGAGGTYTATTA 248T > C (p.Phe83Ser) TGCTTCATGACATAAGACAA′] 1344 NM_001011658.3(TRAPPC2): c. −1 — [′AGTTACCTTTACTAAGAAGGTAAGGRTATGC 238 + 4T > C CCCGCAGTGACAAATGCCGA′] 1345 NM_003688.3(CASK): c.802T > C 8573 CASK [′GGATCCAGCTGAAAGGATCACTGTTYATGA (p.Tyr268His) AGCACTGAATCACCCATGGCT′] 1346 NM_003688.3(CASK): c.2740T > 8573 CASK [′TGAGCTCGTGTGCACAGCCCCACAGYGGGT C (p.Trp914Arg) CCCTGTCTCCTGGGTCTATTA′] 1347 NM_001159702.2(FHL1): c.457T > 2273 FHL1 [′AGGGGAGGACTTCTACTGCGTGACTYGCCA C (p.Cys153Arg) TGAGACCAAGTTTGCCAAGCA′] 1348 NM_001159702.2(FHL1): c.310T > 2273 FHL1 [′GGAGGACTCCCCCAAGTGCAAGGGGYGCTT C (p.Cys104Arg) CAAGGCCATTGTGGCAGGTAC′] 1349 NM_001159702.2(FHL1): c.625T > 2273 FHL1 [′CACCGCTGTGGAGGACCAGTATTACYGCGT C (p.Cys209Arg) GGATTGCTACAAGAACTTTGT′] 1350 NM_001363.4(DKC1): c.113T > C 1736 DKC1 [′ATACAACACGCTGAAGAATTTCTTANCAAA (p.Ile38Thr) CCTGAATCCAAAGTTGCTAAG′] 1351 NM_178152.2(DCX): c.373T > C 1641 DCX [′CTTATTTCTTGCCTTAGGGGAAAGCYATGTC (p.Tyr125His) TGTTCCTCAGACAACTTCTT′] 1352 NM_005183.3(CACNA1F): c.2267T > 778 CACNAF [′ATCCTGTTGAACGTGTTTCTTGCCAYTGCTG C (p.Ile756Thr) TGGACAACCTGGCCAGTGGA′] 1353 NM_001493.2(GDI1): c.275T > C 2664 GDI1 [′ACAGGGCAGCTGGTAAAGATGCTACYGTAT (p.Leu92Pro) ACAGAGGTGACTCGCTACCTG′] 1354 NM_006517.4(SLC16A2): c.1313T > 6567 SLC16A2 [′ATCATGGCCCCCATTGCATTTGAGCYGGTGG C (p.Leu438Pro) GCCCAATGCAGGCCTCACAG′] 1355 NM_006517.4(SLC16A2): c.1190T > 6567 SLC16A2 [′CTCCAGGTCCTTTCCTTCCTGCTCCYGGGCC C (p.Leu397Pro) TGATGTCCATGATGATTCCC′] 1356 NM_006517.4(SLC16A2): c.1481T > 6567 SLC16A2 [′CCCCCCATCATCGGGGCTGTAATCCYCTTCT C (p.Leu494Pro) TCGTCCCTCTGATGCATCAA′] 1357 NM_004586.2(RPS6KA3): c.803T > 6197 RPS6KA3 [′GAAATGCTTACTGGTACACTCCCTTYCCAAG C (p.Phe268Ser) GAAAAGATCGAAAAGAAACA′] 1358 NM_000489.4(ATRX): c.4840T > 546 ATRX [′AAGTTTTCTTCATACAGTTCTTTTGYGTGAC C (p.Cys1614Arg) AAACTGGATTTCAGCACGGC′] 1359 NM_000489.4(ATRX): c.6250T > 546 ATRX [′GAAGTGGCTTCGAAACATTGACTATYACCGT C (p.Tyr2084His) TTAGATGGTTCCACTACTGC′] 1360 NM_000489.4(ATRX): c.1226T > 546 ATRX [′GATATTAAGAAGGCTCATCTTGCATYGGAA C (p.Leu409Ser) GAAGACTTAAATTCCGAGTTT′] 1361 NM_000489.4(ATRX): c.6149T > 546 ATRX [′CAGTCCCTCATATCTCTGGACTTGAYTGAAG C (p.Ile2050Thr) ATTTTCTTGAATTAGCTAGT′] NM_001110556.1(FLNA): c.720 + 2316 FLNA 2T > C 1362 NM_001127899.3(CLCN5): c.1768T > 1184 CLCN5 [′AGGTGGGGTGACTCGGATGACTGTTYCTCTT C (p.Ser590Pro) GTTGTCATAATGTTTGAACT′] 1363 NM_004992.3(MECP2): c.464T > 4204 MECP2 [′GACACATCCCTGGACCCTAATGATTBTGACT C (p.Phe155Ser) TCACGGTAACTGGGAGAGGG′] 1364 NM_001061.4(TBXAS1): c.1463T > 6916 TBXAS1 [′TGCCTCGGGGTGCGTCTAGGGCTGCYTGAG C (p.Leu488Pro) GTCAAGTTGACACTGCTCCAC′] 1365 NM_001061.4(TBXAS1): c.248T > 6916 TBXAS1 [′TTTTTTATTCCTCCCAGGTACTATCYTGGTCG C (p.Leu83Pro) TCGGATGTTTATTGTTATT′] 1366 NM_000128.3(F11): c.901T > C 2160 F11 [′TTCTTCATTTTACCATGACACTGATYTCTTGG (p.Phe301Leu) GAGAAGAACTGGATATTGT′] 1367 NM_000128.3(F11): c.166T > C 2160 F11 [′GGTAGTCTGCACTTACCACCCAAGAYGTTTA (p.Cys56Arg) CTCTTCACTTTCACGGCGGA′] 1368 NM_000203.4(IDUA): c.1469T > 3425 IDUA [′AGCCCCGACGGCGAGTGGCGGCGCCYGGGC C (p.Leu490Pro) CGGCCCGTCTTCCCCACGGCA′] 1369 NM_000431.3(MVK): c.803T > C 4598 MVK [′ATCGTGGCCCCCCTCCTGACCTCAAYAGATG (p.Ile268Thr) CCATCTCCCTGGAGTGTGAG′] 1370 NM_014425.3 (INVS): c.1478T > 27130 INVS [′TTATGCTTATAGGGAAGAACAGCTTYGCATT C (p.Leu493Ser) GGTCCTGCAACAATGGATAC′] 1371 NM_000108.4(DLD): c.1178T > C 1738 DLD [′GACTACAATTGTGTGCCATCAGTGAYTTACA (p.Ile393Thr) CACACCCTGAAGTTGCTTGG′] 1372 NM_000170.2(GLDC): c.2T > C 2731 GLDC [′GGGCAGGGGACGGTGGCCGCGGCCAYGCAG (p.Met1Thr) TCCTGTGCCAGGGCGTGGGGG′] 1373 NM_000151.3(G6PC): c.229T > C 2538 G6PC [′AGACTGGCTCAACCTCGTCTTTAAGYGGTAA (p.Trp77Arg) GAACCATATAGAGAGGAGAT′] 1374 NM_004453.3 (ETFDH): c.2T > C 2110 ETFDH [′CCGAGAGTCCTGGTGACTTTGAACAYGCTG (p.Met1Thr) GTGCCGCTAGCCAAGCTGTCC′] 1375 NM_000136.2(FANCC): c.1661T > −1 — [′CTGGCCCGAGAGCTCCTTAAAGAGCYGCGA C (p.Leu554Pro) ACTCAAGTCTAGAAGGCACGC′] 1376 NM_000131.4(F7): c.38T > C 2155 F7 [′GCCCTCAGGCTCCTCTGCCTTCTGCYTGGGC (p.Leu13Pro) TTCAGGGCTGCCTGGCTGCA′] 1377 NM_000131.4(F7): c.983T > C 2155 F7 [′CCCCTCTGCCTGCCCGAACGGACGTYCTCTG (p.Phe328Ser) AGAGGACGCTGGCCTTCGTG′] 1378 NM_024649.4(BBS1): c.1553T > −1 — [′ACAACCCGTCCTGTCCTGGGGCTGCYGGTCT C (p.Leu518Pro) GCTTCCTGTACAACGAGGCG′] 1379 NM_000490.4(AVP): c.200T > C 551 AVP [′TGCGCGGACGAGCTGGGCTGCTTCGYGGGC (p.Val67Ala) ACGGCTGAGGCGCTGCGCTGC′] 1380 NM_000490.4(AVP): c.61T > C 551 AVP [′CCTACTGGCCTTCTCCTCCGCGTGCYACTTC (p.Tyr21His) CAGAACTGCCCGAGGGGCGG′] 1381 NM_001692.3 (ATP6V1B1): c.242T > 525 ATP6V1B1 [′GGGACTCAGAGGAGCGGGCAGGTGCYTGAG C (p.Leu81Pro) GTGGCTGGCACCAAGGCGATT′] 1382 NM_003361.3 (UMOD): c.649T > 7369 UMOD [′GGGCGGTGCGCGCATGGCCGAGACCBGCGT C (p.Cys217Arg) GCCAGTCCTGCGCTGCAACAC′] 1383 NM_003361.3 (UMOD): c.376T > 7369 UMOD [′TAGCCACTGCCACGCCCTGGCCACAYGTGTC C (p.Cys126Arg) AATGTGGTGGGCAGCTACTT′] 1384 NM_003361.3 (UMOD): c.943T > 7369 UMOD [′CAAATCGAATAATGGCAGATGGCACYGCCA C (p.Cys315Arg) GTGCAAACAGGACTTCAACAT′] 1385 NM_080911.2(UNG): c.752T > C 7374 UNG [′AATCAGAACTCGAATGGCCTTGTTTYCTTGC (p.Phe251Ser) TCTGGGGCTCTTATGCTCAG′] 1386 NM_199292.2(TH): c.707T > C 7054 TH [′CAGGTGTACCGCCAGCGCAGGAAGCYGATT (p.Leu236Pro) GCTGAGATCGCCTTCCAGTAC′] 1387 NM_001065.3(TNFRSF1A): c.175T > 7132 TNFRSF1A [′CCACCCTCAAAATAATTCGATTTGCYGTACC C (p.Cys59Arg) AAGTGCCACAAAGGTAGGGG′] 1388 NM_001065.3(TNFRSF1A): c.349T > 7132 TNFRSF1A [′AATGGGTCAGGTGGAGATCTCTTCTYGCACA C (p.Cys117Arg) GTGGACCGGGACACCGTGTG′] 1389 NM_000546.5(TP53): c.755T > C 7157 TP53 [′GGCGGCATGAACCGGAGGCCCATCCYCACC (p.Leu252Pro) ATCATCACACTGGAAGACTCC′] 1390 NM_000546.5(TP53): c.398T > C 7157 TP53 [′CAGTACTCCCCTGCCCTCAACAAGAYGTTTT (p.Met133Thr) GCCAACTGGCCAAGACCTGC′] 1391 NM_000546.5(TP53): c.1031T > C 7157 TP53 [′GAGCGCTTCGAGATGTTCCGAGAGCYGAAT (p.Leu344Pro) GAGGCCTTGGAACTCAAGGAT′] 1392 NM_001018005.1(TPM1): c.284T > 7168 TPM1 [′TCTCTGAACAGACGCATCCAGCTGGYTGAG C (p.Val95Ala) GAAGAGTTGGATCGTGCCCAG′] 1393 NM_001159287.1(TPI1): c.832T > 7167 TPI1 [′GGGTGGTGCTTCCCTCAAGCCCGAAYTCGTG C (p.Phe278Leu) GACATCATCAATGCCAAACA′] 1394 NM_003242.5(TGFBR2): c.923T > 7048 TGFBR2 [′AAGCATGAGAACATACTCCAGTTCCYGACG C (p.Leu308Pro) GCTGAGGAGCGGAAGACGGAG′] 1395 NM_000660.5(TGFB1): c.673T > 7040 TGFB1 [′CTTTCGCCTTAGCGCCCACTGCTCCYGTGAC C (p.Cys225Arg) AGCAGGGATAACACACTGCA′] 1396 NM_000660.5(TGFB1): c.241T > 7040 TGFB1 [′GCTGCCCGAGGCCGTGCTCGCCCTGYACAA C (p.Tyr81His) CAGCACCCGCGACCGGGTGGC′] 1397 NM_000660.5(TGFB1): c.667T > 7040 TGFB1 [′TGAGGGCTTTCGCCTTAGCGCCCACNGCTCC C (p.Cys223Arg) TGTGACAGCAGGGATAACAC′] 1398 NM_001128177.1(THRB): c.929T > 7068 THRB [′ATCATCCTCCTCAAAGGCTGCTGCAYGGAG C (p.Met310Thr) ATCATGTCCCTTCGCGCTGCT′] 1399 NM_001128177.1(THRB): c.1336T ? 7068 THRB [′CCGCTTCCTGCACATGAAGGTGGAAYGCCC C (p.Cys446Arg) CACAGAACTCTTCCCCCCTTT′] 1400 NM_001128177.1(THRB): c.1373T > 7068 THRB [′CTCTTCCCCCCTTTGTTCTTGGAAGYGTTCG C (p.Val458Ala) AGGATTAGACTGACTGGATT′] 1401 NM_007313.2(ABL1): c.814T > C 25 ABL1 [′GAAGCACAAGCTGGGCGGGGGCCAGYACGG (p.Tyr272His) GGAGGTGTACGAGGGCGTGTG′] 1402 NM_007313.2(ABL1): c.988T > C 25 ABL1 [′AGGGGTCTGCACCCGGGAGCCCCCGYTCTA (p.Phe330Leu) TATCATCACTGAGTTCATGAC′] 1403 NM_007313.2(ABL1): c.1109T > 25 ABL1 [′ATGGCCACTCAGATCTCGTCAGCCAYGGAG C (p.Met370Thr) TACCTGGAGAAGAAAAACTTC′] 1404 NM_021961.5(TEAD1): c.1261T > 7003 TEAD1 [′TGAACACGGAGCACAACATCATATTYACAG C (p.Tyr?His) GCTTGTAAAGGACTGAACATG′] 1405 NM_001256850.1(TTN): c.2926T > 7273 TTN [′TGGATACCCATCCCCGACAGTGACAHGGTA C (p.Trp976Arg) CAGGGAAGACTACCAAATCGA′] 1406 NM_133378.4(TTN): c.100163T > −1 — [′GAAAACACAGATGACCTGACAACCCYGATC C (p.Leu33388Pro) ATCATGGACGTACAGAAACAA′] 1407 NM_003235.4(TG): c.3733T > C 7038 TG [′CCCCACAGGCTCTGCCATGCAGCAGYGCCA (p.Cys1245Arg) ATTGCTGTGCCGCCAGGGCTC′] 1408 NM_003235.4(TG): c.3229T > C 7038 TG [′TCTCTCTCCCACAGGCCCGACAACCYGCGAG (p.Cys1077Arg) AAATCTCGAACCAGTGGGCT′] 1409 NM_000733.3(CD3E): c.520 + 2T > 916 CD3E [′CGGGTGCTGGCGGCAGGCAAAGGGGYAAGG C CTGTGGAGTCCAGTCAGAGGA′] 1410 NM_003000.2(SDHB): c.487T > C 6390 SDHB [′GCCTTATTTGAAGAAGAAGGATGAAYCTCA (p.Ser163Pro) GGAAGGCAAGCAGCAGTATCT′] 1411 NM_003106.3(SOX2): c.290T > C −1 — [′CCGTTCATCGACGAGGCTAAGCGGCYGCGA (p.Leu97Pro) GCGCTGCACATGAAGGAGCAC′] 1412 NM_001024858.2(SPTB): c.604T > 6710 SPTB [′TAATGTCACCAACTTTACCTCCAGCYGGAAG C (p.Trp202Arg) GATGGCTTGGCCTTTAATGC′] 1413 NM_001024858.2(SPTB): c.6055T > 6710 SPTB [′GGTGTGCCAGTTCTCGAGGGATGCCYCTGTG C (p.Ser2019Pro) GCTGAGGCGTGGCTGATTGC′] 1414 NM_003126.2(SPTA1): c.779T > 6708 SPTA1 [′TTGGCTCTCCAGAGACAGAAAGCTCBGTCCA C (p.Leu260Pro) ATGCTGCAAACTTACAACGA′] 1415 NM_003126.2(SPTA1): c.781T > 6708 SPTA1 [′GGCTCTCCAGAGACAGAAAGCTCTGYCCAA C (p.Ser261Pro) TGCTGCAAACTTACAACGATT′] 1416 NM_003126.2(SPTA1): c.620T > 6708 SPTA1 [′AAATTTGAAGACTTCCAAGTGGAGCYGGTA C (p.Leu207Pro) GCTAAAGAAGGGAGAGTTGTT′] 1417 NM_005633.3(SOS1): c.1294T > 6654 SOS1 [′CGAGATTCAGAAGAATATTGATGGTYGGGA C (p.Trp432Arg) GGGAAAAGACATTGGACAGTG′] 1418 NM_006920.4(SCN1A): c.3577T > −1 — [′TGTGGAAGAAGGCAGAGGAAAACAAYGGT C (p.Trp1193Arg) GGAACCTGAGAAGGACGTGTTT′] 1419 NM_006920.4(SCN1A): c.4250T > −1 — [′TTTGGGTATCTCTCTTTGCTTCAAGYTGTAA C (p.Val1417Ala) GTGAACACTATTTTCTCTGA′] 1420 NM_006920.4(SCN1A): c.434T > 6323 SCN1A [′ACTATTTTGACAAACTGTGTGTTTAHGACAA C (p.Met145Thr) TGAGTAACCCTCCTGATTGG′] 1421 NM_006920.4(SCN1A): c.4462T > −1 — [′TCTATACTTTGGAGGTCAAGACATCYTTATG C (p.Phe1488Leu) ACAGAAGAACAGAAGAAATA′] 1422 NM_152296.4(ATP1A3): c.821T > 478 ATP1A3 [′GGGCTGGAGGTGGGCAAGACGCCCAHCGCC C (p.Ile274Thr) ATCGAGATTGAGCACTTCATC′] 1423 NM_152296.4(ATP1A3): c.2338T > 478 ATP1A3 [′CAGCAATATCCCGGAGATCACGCCCYTCCTG C (p.Phe780Leu) CTGTTCATCATGGCCAACAT′] 1424 NM_000702.3(ATP1A2): c.2291T > 477 ATP1A2 [′CCTCAGAATCTCCCCACAGGCCGCCYGATCT C (p.Leu764Pro) TTGACAACTTGAAGAAATCC′] 1425 NM_000702.3(ATP1A2): c.2659T > 477 ATP1A2 [′ACGGCTACTGGGAATCCGCCTCGACYGGGA C (p.Trp887Arg) TGACCGGACCATGAATGATCT′] 1426 NM_000702.3(ATP1A2): c.2192T > 477 ATP1A2 [′AAGAAGGCTGACATTGGCATTGCCAYGGGC C (p.Met731Thr) ATCTCTGGCTCTGACGTCTCT′] 1427 NM_000702.3(ATP1A2): c.857T > 477 ATP1A2 [′GGGCGGACACCCATAGCAATGGAGAYTGAA C (p.Ile286Thr) CACTTCATCCAGCTGATCACA′] 1428 NM_001035.2(RYR2): c.1298T > 6262 RYR2 [′ATGTTTATGGTTTATTTTAGGGGCCYTGATG C (p.Leu433Pro) CTCTCAGCAAGAAAGCGAAG′] 1429 NM_000540.2(RYR1): c.14693T > 6261 RYR1 [′GTGGGTGTCCGGGCTGGCGGAGGCAYTGGG C (p.Ile4898Thr) GACGAGATCGAGGACCCCGCG′] 1430 NM_000539.3(RHO): c.133T > C 6010 RHO [′GTTCTCCATGCTGGCCGCCTACATGYTTCTG (p.Phe45Leu) CTGATCGTGCTGGGCTTCCC′] 1431 NM_000321.2(RB1): c.1960+2T > 5925 RB1 [′CTCTTTCACTGTTTTATAAAAAAGGYTAGTA C GATGATTATTTTCAAGAGCA′] 1432 NM_000321.2(RB1): c.2134T > C 5925 RB1 [′TATGATGTGTTCCATGTATGGCATAYGCAAA (p.Cys712Arg) GTGAAGAATATAGACCTTAA′] 1433 NM_000329.2(RPE65): c.1022T > 6121 RPE65 [′AGATTTGAGTTTGTTTATAATTACTYATATTT C (p.Leu341Ser) AGCCAATTTACGTGAGAAC′] 1434 NM_000322.4(PRPH2): c.554T > 5961 PRPH2 [′ATTCAGTGGATCAGCAATCGCTACCYGGACT C (p.Leu185Pro) TTTCCTCCAAAGAAGTCAAA′] 1435 NM_000322.4(PRPH2): c.2T > C 5961 PRPH2 [′CAACCCGGACTACACTTGGCAAGCAYGGCG (p.Met1Thr) CTACTGAAAGTCAAGTTTGAC′] 1436 NM_001098668.2(SFTPA2): c. 729238 SFTPA2 [′ACTGAGGGTCCCAGCCCTGGAGACTYCCGC 593T > C (p.Phe198Ser) TACTCAGATGGGACCCCTGTA′] 1437 NM_003018.3(SFTPC): c.218T > 6440 SFTPC [′TTTCCCCAGGTTCTGGAGATGAGCAHTGGGG C (p.Ile73Thr) CGCCGGAAGCCCAGCAACGC′] 1438 NM_003018.3 (SFTPC): c.581T > 6440 SFTPC [′AGCACCCTGTGTGGCGAGGTGCCGCYCTACT C (p.Leu194Pro) ACATCTAGGACGCCTCCGGT′] 1439 NM_000055.2(BCHE): c.1004T > 590 BCHE [′CTCACTGACATGCCAGACATATTACYTGAAC C (p.Leu335Pro) TTGGACAATTTAAAAAAACC′] 1440 NM_002739.3(PRKCG): c.355T > 5582 PRKCG [′CCCCACCTTCTGCGACCACTGTGGCYCCCTC C (p.Ser119Pro) CTCTACGGGCTTGTGCACCA′] 1441 NM_002739.3(PRKCG): c.1927T > 5582 PRKCG [′ACAGTGTGGCCGCAGCGGCGAGAACYTTGA C (p.Phe643Leu) CAAGTTCTTCACGCGGGCGGC′] 1442 NM_000141.4(FGFR2): c.1018T > 2263 FGFR2 [′TGTAACTTTTGAGGACGCTGGGGAAYATAC C (p.Tyr340His) GTGCTTGGCGGGTAATTCTAT′] 1443 NM_000141.4(FGFR2): c.1024T > 2263 FGFR2 [′TTTTGAGGACGCTGGGGAATATACGHGCTTG C (p.Cys342Arg) GCGGGTAATTCTATTGGGAT′] 1444 NM_000141.4(FGFR2): c.868T > 2263 FGFR2 [′CAGTGATGCCCAGCCCCACATCCAGBGGAT C (p.Trp290Arg) CAAGCACGTGGAAAAGAACGG′] 1445 NM_000141.4(FGFR2): c.799T > 2263 FGFR2 [′CCAAGCCGGACTGCCGGCAAATGCCYCCAC C (p.Ser267Pro) AGTGGTCGGAGGAGACGTAGA′] 1446 NM_000506.3(F2): c.1139T > C 2147 F2 [′CCTGCTGCCCCTCCCAGGCAGGTGAYGCTTT (p.Met380Thr) TCCGGAAGAGTCCCCAGGAG′] 1447 NM_000313.3 (PROS1): c.1501T > 5627 PROS1 [′TTCATTATTTTAAATAGATAATGTABCCAGT C (p.Ser501Pro) GCTGAGGGTTGGCATGTAAA′] 1448 NM_002834.3(PTPN11): c.854T > 5781 PTPN11 [′TGACCGTGGTCTCTTTTTCTTCTAGNTGATCA C (p.Phe285Ser) TACCAGGGTTGTCCTACAC′] 1449 NM_002755.3(MAP2K1): c.158T > 5604 MAP2K1 [′CAGCAGCGAAAGCGCCTTGAGGCCTYTCTT C (p.Phe53Ser) ACCCAGAAGCAGAAGGTGGGA′] 1450 NM_001042465.1(PSAP): c.1055T > 5660 PSAP [′GCTTTTGACAAAATGTGCTCGAAGCYGCCG C (p.Leu352Pro) AAGTCCCTGTCGGAAGAGTGC′] 1451 NM_000207.2(INS): c.143T > C −1 — [′TACCTAGTGTGCGGGGAACGAGGCTBCTTCT (p.Phe48Ser) ACACACCCAAGACCCGCCGG′] 1452 NM_000311.3(PRNP): c.593T > C 5621 PRNP [′ACCACAACCACCAAGGGGGAGAACTYCACC (p.Phe198Ser) GAGACCGACGTTAAGATGATG′] 1453 NM_000371.3(TTR): c.149T > C 7276 TTR [′GGCAGTCCTGCCATCAATGTGGCCGBGCATG (p.Val50Ala) TGTTCAGAAAGGCTGCTGAT′] 1454 NM_000371.3(TTR): c.224T > C 7276 TTR [′AGGAAAACCAGTGAGTCTGGAGAGCYGCAT (p.Leu75Pro) GGGCTCACAACTGAGGAGGAA′] 1455 NM_000371.3(TTR): c.88T > C 7276 TTR [′CACCCAGGGCACCGGTGAATCCAAGYGTCC (p.Cys30Arg) TCTGATGGTCAAAGTTCTAGA′] 1456 NM_000371.3(TTR): c.272T > C 7276 TTR [′GAATTTGTAGAAGGGATATACAAAGYGGAA (p.Val91Ala) ATAGACACCAAATCTTACTGG′] 1457 NM_000371.3(TTR): c.400T > C 7276 TTR [′CACCATTGCCGCCCTGCTGAGCCCCYACTCC (p.Tyr134His) TATTCCACCACGGCTGTCGT′] 1458 NM_000371.3(TTR): c.250T > C 7276 TTR [′GCATGGGCTCACAACTGAGGAGGAAYTTGT (p.Phe84Leu) AGAAGGGATATACAAAGTGGA′] 1459 NM_000371.3(TTR): c.157T > C 7276 TTR [′TGCCATCAATGTGGCCGTGCATGTGHTCAGA (p.Phe53Leu) AAGGCTGCTGATGACACCTG′] 1460 NM_000371.3(TTR): c.95T > C 7276 TTR [′GGCACCGGTGAATCCAAGTGTCCTCYGATG (p.Leu32Pro) GTCAAAGTTCTAGATGCTGTC′] 1461 NM_000371.3(TTR): c.191T > C 7276 TTR [′GCTGCTGATGACACCTGGGAGCCATYTGCCT (p.Phe64Ser) CTGGGTAAGTTGCCAAAGAA′] 1462 NM_000371.3(TTR): c.265T > C 7276 TTR [′TGAGGAGGAATTTGTAGAAGGGATAYACAA (p.Tyr89His) AGTGGAAATAGACACCAAATC′] 1463 NM_000217.2(KCNA1): c.1223T > 3736 KCNA1 [′ATTGCCCTGCCCGTACCTGTCATTGYGTCCA C (p.Val408Ala) ATTTCAACTATTTCTACCAC′] 1464 NM_000174.4(GP9): c.212T > C 2815 GP9 [′CTTCAGTCCGTGCCCCCGGGAGCCTBTGACC (p.Phe71Ser) ACCTGCCCCAGCTGCAGACC′] 1465 NM_000174.4(GP9): c.167T > C 2815 GP9 [′GCCCTGCCGGCCCGCACCCGCCACCYTCTGC (p.Leu56Pro) TGGCCAACAACAGCCTTCAG′] 1466 NM_000174.4(GP9): c.70T > C 2815 GP9 [′GGCCACCAAGGACTGCCCCAGCCCAYGTAC (p.Cys24Arg) CTGCCGCGCCCTGGAAACCAT′] 1467 NM_000174.4(GP9): c.20T > C 2815 GP9 [′TGTCCCATGCCTGCCTGGGGAGCCCYGTTCC (p.Leu7Pro) TGCTCTGGGCCACAGCAGAG′] 1468 NM_001001547.2(CD36): c.760T > 948 CD36 [′CGATTTTTAAACAGATGCAGCCTCABTTCCA C (p.Phe254Leu) CCTTTTGTTGAGAAAAGCCA′] 1469 NM_000212.2(ITGB3): c.2332T > −1 — [′CAACCCACTGTATAAAGAGGCCACGYCTAC C (p.Ser778Pro) CTTCACCAATATCACGTACCG′] 1470 NM_000212.2(ITGB3): c.176T > 3690 ITGB3 [′GCTCCTGTCTTACAGGCCCTGCCTCYGGGCT C (p.Leu59Pro) CACCTCGCTGTGACCTGAAG′] 1471 NM_000301.3(PLG): c.1771T > C 5340 PLG [′AGGGGGGTGTGTGGCCCACCCACATYCCTG (p.Ser591Pro) GCCCTGGCAAGTCAGTCTTAG′] 1472 NM_001122757.2(POU1F1): c. 5449 POU1F1 [′CAAACTGAAAGCAATATTATCCAAAYGGCT 655T > C (p.Trp219Arg) GGAGGAAGCTGAGCAAGTAGG′] 1473 NM_000293.2(PHKB): c.2923T > 5257 PHKB [′ACCAACCCTGTCAGATATGACCATGHATGA C (p.Tyr975His) GATGAATTTCTCTCTCCTTGT′] 1474 NM_004577.3(PSPH): c.155T > C 5723 PSPH [′CTCCCTCCTAGGACACGGCGAGCCAYGGGC (p.Met52Thr) GGGGCAGTGCCTTTCAAAGCT′] 1475 NM_000175.3(GPI): c.1574T > C 2821 GPI [′CTGGGAAAGCAGCTGGCTAAGAAAABAGAG (p.Ile525Thr) CCTGAGCTTGATGGCAGTGCT′] 1476 NM_000175.3(GPI): c.1016T > C 2821 GPI [′TTTGGGTGTGAGACACACGCCATGCYGCCCT (p.Leu339Pro) ATGACCAGTACCTGCACCGC′] 1477 NM_000478.4(ALPL): c.1306T > 249 ALPL [′ACGAGAGAATGTCTCCATGGTGGACYATGG C (p.Tyr436His) TGAGACCTCCAGGACCCAGGG′] 1478 NM_000478.4(ALPL): c.979T > C 249 ALPL [′GATCCTGCGGAAGAACCCCAAAGGCYTCTT (p.Phe327Leu) CTTGCTGGTGGAAGGTAGGGA′] 1479 NM_018849.2(ABCB4): c.1207T > 5244 ABCB4 [′GGAGTTCAATGATGTTCACTTTTCTYACCCT C (p.Tyr403His) TCTCGAGCTAACGTCAAGGT′] 1480 NM_198965.1(PTHLH): c.179T > 5744 PTHLH [′CAAGATTTACGGCGACGATTCTTCCYTCACC C (p.Leu60Pro) ATCTGATCGCAGAAATCCAC′] 1481 NM_198965.1(PTHLH): c.131T > 5744 PTHLH [′AGAGCTGTGTCTGAACATCAGCTCCYCCATG C (p.Leu44Pro) ACAAGGGGAAGTCCATCCAA′] 1482 NM_000315.2(PTH): c.52T > C 5741 PTH [′AGTTATGATTGTCATGTTGGCAATTYGTTTT (p.Cys18Arg) CTTACAAAATCGGATGGGAA′] 1483 NM_000315.2(PTH): c.67T > C 5741 PTH [′GTTGGCAATTTGTTTTCTTACAAAAYCGGAT (p.Ser23Pro) GGGAAATCTGTTAAGTAAGT′] 1484 NM_003122.4(SPINK1): c.2T > C 6690 SPINK1 [′ACCTCTGGACGCAGAACTTCAGCCAYGAAG (p.Met1Thr) GTAACAGGCATCTTTCTTCTC′] 1485 NM_003122.4(SPINK1): c.41T > 6690 SPINK1 [′ATCTTTCTTCTCAGTGCCTTGGCCCBGTTGA C (p.Leu14Pro) GTCTATCTGGTAAGTGTTGC′] 1486 NM_006194.3(PAX9): c.62T > C 5083 PAX9 [′GGAGTGTTCGTGAACGGGAGGCCGCYGCCC (p.Leu21Pro) AACGCCATCCGGCTTCGCATC′] 1487 NM_005188.3(CBL): c.1111T > C 867 CBL [′TTAATCAAAGGAACAATATGAATTAHACTG (p.Tyr371His) TGAGATGGGCTCCACATTCCA′] 1488 NM_005247.2(FGF3): c.466T > C 2248 FGF3 [′CAGCGCCGAGAGACTGTGGTACGTGYCTGT (p.Ser156Pro) GAACGGCAAGGGCCGGCCCCG′] 1489 NM_005247.2(FGF3): c.17T > C 2248 FGF3 [′GATGCCACGATGGGCCTAATCTGGCYGCTA (p.Leu6Pro) CTGCTCAGCCTGCTGGAGCCC′] 1490 NM_000222.2(KIT): c.1676T > C 3815 KIT [′CCCATGTATGAAGTACAGTGGAAGGNTGTT (p.Val559Ala) GAGGAGATAAATGGAAACAAT′] 1491 NM_005249.4(FOXG1): c.643T > 2290 FOXG1 [′CATCTACGAGTTCATCATGAAGAACYTCCCT C (p.Phe215Leu) TACTACCGCGAGAACAAGCA′] 1492 NM_001127500.1(MET): c.3446T > 4233 MET [′CAATTTCTGACCGAGGGAATCATCAYGAAA C (p.Met1149Thr) GATTTTAGTCATCCCAATGTC′] 1493 NM_020975.4(RET): c.1858T > C 5979 RET [′CTTCCCTGAGGAGGAGAAGTGCTTCNGCGA (p.Cys620Arg) GCCCGAAGACATCCAGGGTGA′] 1494 NM_020975.4(RET): c.1900T > C 5979 RET [′CACAGATCCACTGTGCGACGAGCTGNGCCG (p.Cys634Arg) CACGGTGATCGCAGCCGCTGT′] 1495 NM_020975.4(RET): c.2753T > C 5979 RET [′TAGGGTCGGATTCCAGTTAAATGGAYGGCA (p.Met918Thr) ATTGAATCCCTTTTTGATCAT′] 1496 NM_020630.4(RET): c.1852T > C 5979 RET [′CAACTGCTTCCCTGAGGAGGAGAAGNGCTT (p.Cys618Arg) CTGCGAGCCCGAAGACATCCA′] 1497 NM_020630.4(RET): c.1825T > C 5979 RET [′GGGGATTAAAGCTGGCTATGGCACCNGCAA (p.Cys609Arg) CTGCTTCCCTGAGGAGGAGAA′] 1498 NM_013251.3(TAC3): c.269T > C 6866 TAC3 [′ATGCATGACTTCTTTGTGGGACTTAYGGGCA (p.Met90Thr) AGAGGAGCGTCCAGCCAGGT′] 1499 NM_006158.4(NEFL): c.281T > C 4747 NEFL [′ATCCGCACGCAGGAGAAGGCGCAGCYCCAG (p.Leu94Pro) GACCTCAATGACCGCTTCGCC′] 1500 NM_006177.3(NRL): c.479T > C 4901 NRL [′TGCGGGCGCGACGAGGCGCTGCGGCYGAAG (p.Leu160Pro) CAGAGGCGCCGCACGCTGAAG′] 1501 NM_000432.3(MYL2): c.52T > C 4633 MYL2 [′AGCCGGGGGCGCCAACTCCAACGTGYTCTC (p.Phe18Leu) CATGTTCGAACAGACCCAAAT′] 1502 NM_000257.3(MYH7): c.1046T > 4625 MYH7 [′TTCACTTCAGAGGAGAAAAACTCCAYGTAT C (p.Met349Thr) AAGCTGACAGGCGCCATCATG′] 1503 NM_000257.3(MYH7): c.1594T > 4625 MYH7 [′GCTTCCTCAGCCCATGGGCATCATGYCCATC C (p.Ser532Pro) CTGGAAGAGGAGTGCATGTT′] 1504 NM_000257.3(MYH7): c.5378T > 4625 MYH7 [′AACATGGAACAGACCATTAAGGACCYGCAG C (p.Leu1793Pro) CACCGGCTGGACGAAGCCGAG′] 1505 NM_001040113.1(MYH11): c. 4629 MYH11 [′GAGGTGGAACATAAGAAGAAGAAGCYGGA 3791T > C (p.Leu1264Pro) GGCGCAGGTGCAGGAGCTGCAG′] 1506 NM_000530.6(MPZ): c.404T > C 4359 MPZ [′TGTGACGTCAAAAACCCTCCAGACAYAGTG (p.Ile135Thr) GGCAAGACCTCTCAGGTCACG′] 1507 NM_000530.6(MPZ): c.341T > C 4359 MPZ [′CGCTGGAAGGATGGCTCCATTGTCAYACAC (p.Ile114Thr) AACCTAGACTACAGTGACAAT′] 1508 NM_016835.4(MAPT): c.1839T > 4137 MAPT [′AGTCCAAGTGTGGCTCAAAGGATAAYATCA C (p.Asn613=) AACACGTCCCGGGAGGCGGCA′] 1509 NM_198159.2(MITF): c.1051T > 4286 MITF [′GAACAAGGGAACCATCTTAAAAGCAYCCGT C (p.Ser351Pro) GGACTATATCCGAAAGTTGCA′] 1510 NM_198159.2(MITF): c.1195T > 4286 MITF [′AATGCAGGCTCGAGCTCATGGACTTYCCCTT C (p.Ser399Pro) ATTCCATCCACGGGTCTCTG′] 1511 NM_000426.3 (LAMA2): c.7691T > 3908 LAMA2 [′ACCAAGAATGAGTCCGGCATCATTCYTTTGG C (p.Leu2564Pro) GAAGTGGAGGGACACCAGCA′] 1512 NM_000426.3(LAMA2): c.2584T > 3908 LAMA2 [′ACAACCCTCTGTACCTGGAGGATCAYGTCA C (p.Cys862Arg) GCCATGCCAATGCAATGACAA′] 1513 NM_002435.2(MPI): c.413T > C 4351 MPI [′CCCGATGCCAACCACAAGCCAGAGAYGGCC (p.Met138Thr) ATTGCCCTCACCCCCTTCCAG′] 1514 NM_000239.2(LYZ): c.221T > C 4069 LYZ [′GGAGACAGAAGCACTGATTATGGGAYATTT (p.Ile74Thr) CAGATCAATAGCCGCTACTGG′] 1515 NM_000239.2(LYZ): c.244T > C 4069 LYZ [′GATATTTCAGATCAATAGCCGCTACHGGTGT (p.Trp82Arg) AATGATGGCAAAACCCCAGG′] 1516 NM_000233.3(LHCGR): c.1627T > −1 — [CAATGTGGTGGCCTTCTTCATAATTYGTGCT C (p.Cys543Arg) TGCTACATTAAAATTTATTT′] 1517 NM_000233.3(LHCGR): c.1193T > −1 — [′AAACTTACAGTGCCTCGTTTTCTCAYGTGCA C (p.Met398Thr) ATCTCTCCTTTGCAGACTTT′] 1518 NM_000233.3 (LHCGR): c.391T > −1 — [′TCCTGTCCCTAATCACAGGAGCATCYGTAAC C (p.Cys131Arg) ACAGGCATCAGAAAGTTTCC′] 1519 NM_000233.3(LHCGR): c.1103T > −1 — [′GACTTCCTTAGGGTCCTGATTTGGCYGATTA C (p.Leu368Pro) ATATTCTAGCCATCATGGGA′] 1520 NM_000233.3(LHCGR): c.1505T > −1 — [′TCTTCTCTAATTGCTATGTTGCCCCYTGTCGG C (p.Leu502Pro) TGTCAGCAATTACATGAAG′] 1521 NM_170707.3(LMNA): c.1589T > 4000 LMNA [′TGCGGGAACAGCCTGCGTACGGCTCYCATC C (p.Leu530Pro) AACTCCACTGGGGAAGTAAGT′] 1522 NM_170707.3(LMNA): c.1139T > 4000 LMNA [′GAGATCCACGCCTACCGCAAGCTCTYGGAG C (p.Leu380Ser) GGCGAGGAGGAGAGGTGGGCT′] 1523 NM_002292.3(LAMB2): c.961T > 3913 LAMB2 [′CAAACACAACACACGTGGCCTCAACYGCGA C (p.Cys321Arg) GCAGTGTCAGGATTTCTATCG′] 1524 NM_000228.2(LAMB3): c.565 − 3914 LAMB3 [′AAATCCATAAGGTTAAGTTGGACCTRCAGA 3T > C GGGAAGGGAAAGAGAAGCGCT′] 1525 NM_000421.3(KRT10): c.482T > −1 — [CTGAATGACCGCCTGGCTTCCTACTYGGACA C (p.Leu161Ser) AAGTTCGGGCTCTGGAAGAA′] 1526 NM_000421.3(KRT10): c.449T > −1 — [′CTCTCTGGAAATGAAAAAGTAACCABGCAG C (p.Met150Thr) AATCTGAATGACCGCCTGGCT′] 1527 NM_000422.2(KRT17): c.263T > 3872 KRT17 [′CTGGCTGGAGGTGAGAAGGCCACCAHGCAG C (p.Met88Thr) AACCTCAATGACCGCCTGGCC′] 1528 NM_000422.2(KRT17): c.284T > 3872 KRT17 [′ACCATGCAGAACCTCAATGACCGCCHGGCC C (p.Leu95Pro) TCCTACCTGGACAAGGTGCGT′] 1529 NM_000422.2(KRT17): c.296T > 3872 KRT17 [′CTCAATGACCGCCTGGCCTCCTACCYGGACA C (p.Leu99Pro) AGGTGCGTGCCCTGGAGGAG′] 1530 NM_005557.3(KRT16): c.395T > 3868 KRT16 [′CTCAATGACCGCCTGGCCTCCTACCYGGACA C (p.Leu132Pro) AGGTGCGTGCTCTGGAGGAG′] 1531 NM_005557.3(KRT16): c.362T > 3868 KRT16 [′CTGGTGGGCAGTGAGAAGGTGACCAHGCAG C (p.Met121Thr) AACCTCAATGACCGCCTGGCC′] 1532 NM_000526.4(KRT14): c.1151T > 3861 KRT14 [′ATGATTGGCAGCGTGGAGGAGCAGCYGGCC C (p.Leu384Pro) CAGCTCCGCTGCGAGATGGAG′] 1533 NM_000526.4(KRT14): c.356T > 3861 KRT14 [′CTGGTGGGCAGTGAGAAGGTGACCAYGCAG C (p.Met119Thr) AACCTCAATGACCGCCTGGCC′] 1534 NM_000526.4(KRT14): c.1243T > 3861 KRT14 [′GCGGCTGGAGCAGGAGATCGCCACCYACCG C (p.Tyr415His) CCGCCTGCTGGAGGGCGAGGA′] 1535 NM_153490.2(KRT13): c.356T > 3860 KRT13 [′CTCAACGACCGCCTGGCTTCCTACCYGGAGA C (p.Leu119Pro) AGGTGCGCGCCCTGGAGGAG′] 1536 NM_002273.3(KRT8): c.160T > C 3856 KRT8 [′CTTTCGCGGTGGCCTGGGCGGCGGCHATGGT (p.Tyr54His) GGGGCCAGCGGCATGGGAGG′] 1537 NM_000424.3(KRT5): c.1388T > 3852 KRT5 [′GAGCTCATGAACACCAAGCTGGCCCYGGAC C (p.Leu463Pro) GTGGAGATCGCCACTTACCGC′] 1538 NM_000424.3(KRT5): c.980T > C 3852 KRT5 [′TCTGACACCTCAGTGGTCCTCTCCAHGGACA (p.Met327Thr) ACAACCGCAACCTGGACCTG′] 1539 NM_000424.3(KRT5): c.20T > C 3852 KRT5 [′GCCACCATGTCTCGCCAGTCAAGTGYGTCCT (p.Val7Ala) TCCGGAGCGGGGGCAGTCGT′] 1540 NM_000424.3(KRT5): c.541T > C 3852 KRT5 [′CAAGACCCTCAACAATAAGTTTGCCYCCTTC (p.Ser181Pro) ATCGACAAGGTGAGCTACGA′] 1541 NM_000208.2(INSR): c.779T > C 3643 INSR [′TGCGTGGCCTGCCGCAACTTCTACCYGGACG (p.Leu260Pro) GCAGGTGTGTGGAGACCTGC′] 1542 NM_000208.2(INSR): c.164T > C 3643 INSR [′TTGCATGAGCTGGAGAATTGCTCTGYCATCG (p.Val55Ala) AAGGACACTTGCAGATACTC′] 1543 NM_002198.2(IRF1): c.31T > C 3659 IRF1 [′CATCACTCGGATGCGCATGAGACCCYGGCT (p.Trp11Arg) AGAGATGCAGATTAATTCCAA′] 1544 NM_000213.3(ITGB4): c.467T > 3691 ITGB4 [′AACCTCAAGAAGATGGGGCAGAACCYGGGT C (p.Leu156Pro) ACGGCAGGGCCAGAGTGGAGG′] 1545 NM_000213.3(ITGB4): c.1684T > 3691 ITGB4 [′CCGAGGACGCTGCTCCATGGGCCAGYGTGT C (p.Cys562Arg) GTGTGAGCCTGGTTGGACAGG′] 1546 NM_000213.3 (ITGB4): c.112T > 3691 ITGB4 [′CTGCAAGAAGGCCCCAGTGAAGAGCYGCAC C (p.Cys38Arg) GGAGTGTGTCCGTGTGGATAA′] 1547 NM_000454.4(SOD1): c.338T > C 6647 SOD1 [′ATCTCACTCTCAGGAGACCATTGCAYCATTG (p.Ile113Thr) GCCGCACACTGGTGGTAAGT′] 1548 NM_000454.4(SOD1): c.434T > C 6647 SOD1 [′AAGACAGGAAACGCTGGAAGTCGTTYGGCT (p.Leu145Ser) TGTGGTGTAATTGGGATCGCC′] 1549 NM_000454.4(SOD1): c.455T > C 6647 SOD1 [′CGTTTGGCTTGTGGTGTAATTGGGAYCGCCC (p.Ile152Thr) AATAAACATTCCCTTGGATG′] 1550 NM_001025107.2(ADAR): c.1883T > 103 ADAR [′ACCAGACTTTCTTTTTGTAGGTTTCYCTACA C (p.Leu628Pro) GTGAGTTAATGAAATACAAC′] 1551 NM_001025107.2(ADAR): c.2609T > 103 ADAR [′TCCAAAAAGAACATTTTTCTTCTATYTAAGA C (p.Phe870Ser) AGCTCTGCTCCTTCCGTTAC′] 1552 NM_002185.3(IL7R): c.197T > C 3575 UL7R [′CCATATTTCAAATTCCAGATTGGTGRTGTTG (p.Ile66Thr) ACATCTGGGTCCTCAAAAGC′] 1553 NM_033500.2(HK1): c.1550T > C 3098 HK1 [′ACTGCAACAGAGAATGGTGACTTCTYGGCC (p.Leu517Ser) CTGGATCTTGGAGGAACCAAT′] 1554 NM_000184.2(HB G2): c.- 3048 HBG2 [′CTTCCCCACACTATCTCAATGCAAAYATCTG 228T > C TCTGAAACGGTCCCTGGCTA′] 1555 NM_000184.2(HB G2): c.125T > C 3048 HBG2 [′GTTGTCTACCCATGGACCCAGAGGTYCTTTG (p.Phe42Ser) ACAGCTTTGGCAACCTGTCC′] 1556 NM_000559.2(HB G1): c.- 3047 HBG1 [′TAGCAGTATCCTCTTGGGGGCCCCTYCCCCA 251T > C CACTATCTCAATGCAAATAT′] 1557 NM_000519.3(HBD): c.-127T > C 3045 HBD [′TCTCACAAACTAATGAAACCCTGCTYATCTT AAACCAACCTGCTCACTGGA′] 1558 NM_000518.4(HBB): c.257T > C 3043 HBB [′CACCTGGACAACCTCAAGGGCACCTYTGCC (p.Phe86Ser) ACACTGAGTGAGCTGCACTGT′] 1559 NM_000518.4(HBB): c.128T > C 3043 HBB [′GTCTACCCTTGGACCCAGAGGTTCTBTGAGT (p.Phe43Ser) CCTTTGGGGATCTGTCCACT′] 1560 NM_000518.4(HBB): c.337T > C 3043 HBB [′ACAGCTCCTGGGCAACGTGCTGGTCYGTGTG (p.Cys113Arg) CTGGCCCATCACTTTGGCAA′] 1561 NM_000518.4(HBB): c.127T > C 3043 HBB [′GGTCTACCCTTGGACCCAGAGGTTCBTTGAG (p.Phe43Leu) TCCTTTGGGGATCTGTCCAC′] 1562 NM_000518.4(HBB): c.332T > C 3043 HBB [′CTCCCACAGCTCCTGGGCAACGTGCYGGTCT (pLeu111Pro) GTGTGCTGGCCCATCACTTT′] 1563 NM_000518.4(HBB): c.92 + 6T > C 3043 HBB [′GGTGGTGAGGCCCTGGGCAGGTTGGHATCA AGGTTACAAGACAGGTTTAAG′] 1564 NM_000518.4(HBB): c.344T > C 3043 HBB [′CTGGGCAACGTGCTGGTCTGTGTGCYGGCCC (p.Leu115Pro) ATCACTTTGGCAAAGAATTC′] 1565 NM_000517.4(HBA2): c.427T > C 3040 HBA2 [′CACCGTGCTGACCTCCAAATACCGTNAAGCT (p.Ter143Gln) GGAGCCTCGGTAGCCGTTCC′] 1566 NM_000517.4(HBA2): c.2T > C 3040 HBA2 [′CCACAGACTCAGAGAGAACCCACCAYGGTG (p.Met1Thr) CTGTCTCCTGCCGACAAGACC′] 1567 NM_000517.4(HBA2): c.89T > C 3040 HBA2 [′GCTGGCGAGTATGGTGCGGAGGCCCYGGAG (p.Leu30Pro) AGGTGAGGCTCCCTCCCCTGC′] 1568 NM_006121.3(KRT1): c.482T > C 3848 KRT1 [′CAAGAAGTCACTATCAACCAGAGCCYTCTTC (p.Leu161Pro) AGCCCCTCAATGTGGAGATT′] 1569 NM_006121.3(KRT1): c.1436T > 3848 KRT1 [′ACCAAGCTGGCCCTGGATCTGGAGAYTGCC C (p.Ile479Thr) ACCTACAGGACCCTCCTGGAG′] 1570 NM_006121.3(KRT1): c.1424T > 3848 KRT1 [′GAGCTGATGAACACCAAGCTGGCCCYGGAT C (p.Leu475Pro) CTGGAGATTGCCACCTACAGG′] 1571 NM_001077488.3(GNAS): c.299T > 2778 GNAS [′AAAGTGCAGGACATCAAAAACAACCYGAAA C (p.Leu100Pro) GAGGCGATTGAAGTACGTGCT′] 1572 NM_000515.4(GH1): c.291 + 6T > 2688 GH1 [′AGGAAACACAACAGAAATCCGTGAGYGGAT C GCCTTCTCCCCAGGCGGGGAT′] 1573 NM_002087.3(GRN): c.2T > C 2896 GRN [′TCCTTGGTACTTTGCAGGCAGACCAYGTGGA (p.Met1Thr) CCCTGGTGAGCTGGGTGGCC′] 1574 NM_021957.3(GYS2): c.1447T > 2998 GYS2 [′GGTGATTTTGCACCCAGAGTTTCTAYCCTCC C (p.Ser483Pro) ACCAGTCCCTTACTACCCAT′] 1575 NM_001083112.2(GPD2): c.1904T > 2820 GPD2 [′AGGTATAAGAAGAGATTTCATAAGTYTGAT C (p.Phe635Ser) GCAGACCAGAAAGGCTTTATT′] 1576 NM_000340.1(SLC2A2): c.1166T > 6514 SLC2A2 [′ATCTTCATGTCAGTGGGACTTGTGCBGCTGG C (p.Leu389Pro) TAAGTTTGGTGCCTGCACTG′] 1577 NM_005271.3(GLUD1): c.1501T > 2746 GLUD1 [′GTGTTCTTTTCCCTAATAGGGTGCAYCTGAG C (p.Ser501Pro) AAAGACATCGTGCACTCTGG′] 1578 NM_000162.3(GCK): c.391T > C 2645 GCK [′CTTCGACTACATCTCTGAGTGCATCYCCGAC (p.Ser131Pro) TTCCTGGACAAGCATCAGAT′] 1579 NM_001018077.1(NR3C1): c.1712T > 2908 NR3C1 [′CTCAACATGTTAGGAGGGCGGCAAGYGATT C (p.Val571Ala) GCAGCAGTGAAATGGGCAAAG′] 1580 NM_001018077.1(NR3C1): c.2318T > 2908 NR3C1 [′TCAAATGGAAATATCAAAAAACTTCYGTTTC C (p.Leu773Pro) ATCAAAAGTGACTGCCTTAA′] 1581 NM_001018077.1(NR3C1): c.2209T > 2908 NR3C1 [′GGTTGAAAATCTCCTTAACTATTGCYTCCAA C (p.Phe737Leu) ACATTTTTGGATAAGACCAT′] 1582 NM_002055.4(GFAP): c.1055T > 2670 GFAP [′CGCCACTTGCAGGAGTACCAGGACCYGCTC C (p.Leu352Pro) AATGTCAAGCTGGCCCTGGAC′] 1583 NM_002353.2(TACSTD2): c.557T > 4070 TACSTD2 [′CGGCTCTTCCGCGAGCGCTATCGGCYGCACC C (p.Leu186Pro) CCAAGTTCGTGGCGGCCGTG′] 1584 NM_000821.6(GGCX): c.896T > 2677 GGCX [′TTTCTCCCTGCTTTCCTAGGTATGTYCTCCTA C (p.Phe299Ser) CGTCATGCTGGCCAGCAGC′] 1585 NM_000145.3(FSHR): c.479T > C 2492 FSHR [′CAAGATAACATAAACATCCACACAAYTGAA (p.Ile160Thr) AGAAATTCTTTCGTGGGGCTG′] 1586 NM_000145.3(FSHR): c.1634T > 2492 FSHR [′CTCAATGTCCTGGCCTTTGTGGTCAYCTGTG C (p.Ile545Thr) GCTGCTATATCCACATCTAC′] 1587 NM_182925.4(FLT4): c.3131T > C 2324 FLT4 [′AGAGACCTGGCTGCTCGGAACATTCYGCTGT (p.Leu1044Pro) CGGAAAGCGACGTGGTGAAG′] 1588 NM_182925.4(FLT4): c.3257T > C 2324 FLT4 [′CTGAAGTGGATGGCCCCTGAAAGCAYCTTC (p.Ile1086Thr) GACAAGGTGTACACCACGCAG′] 1589 NM_023110.2(FGFR1): c.899T > 2260 FGFR1 [′CACATCGAGGTGAATGGGAGCAAGAYTGGC C (p.Ile300Thr) CCAGACAACCTGCCTTATGTC′] 1590 NM_023110.2(FGFR1): c.1141T > 2260 FGFR1 [′CCTGTACCTGGAGATCATCATCTATYGCACA C (p.Cys381Arg) GGGGCCTTCCTCATCTCCTG′] 1591 NM_000138.4(FBN1): c.4987T > 2200 FBN1 [′TCCTGGAATCTGTGGTCCAGGGACAYGTTAC C (p.Cys1663Arg) AACACCGTTGGCAACTACAC′] 1592 NM_000138.4(FBN1): c.3220T > 2200 FBN1 [′CTGTTTTTGTGCAGACATTGACGAAYGCCGC C (p.Cys1074Arg) ATATCTCCTGACCTCTGTGG′] 1593 NM_000138.4(FBN1): c.3793T > 2200 FBN1 [′TATCCCTGGAGAGTACAGGTGCTTGYGTTAT C (p.Cys1265Arg) GATGGATTCATGGCATCTGA′] 1594 NM_000043.4(FAS): c.532T > C 355 FAS [′ATCCAGATCTAACTTGGGGTGGCTTYGTCTT (p.Cys178Arg) CTTCTTTTGCCAATTCCACT′] 1595 NM_000043.4(FAS): c.651 + 2T > 355 FAS [′CATGAATCTCCAACTTTAAATCCTGHAGGTA C TTGAAATAGGTATCAGCTTT′] 1596 NM_000129.3(F13A1): c.728T > C 2162 F13A1 [′ATCCTGGACACTTGCCTGTATGTGAYGGACA (p.Met243Thr) GAGCACAAATGGACCTCTCT′] 1597 NM_000186.3(CFH): c.1606T > C 3075 CFH [′GCTGAATGACACATTGGACTATGAAYGCCA (p.Cys536Arg) TGATGGTTATGAAAGCAATAC′] 1598 NM_000123.3(ERCC5): c.2573T > −1 — [′AAGTTAATAAATTTGGCTTATTTGCYTGGAA C (p.Leu858Pro) GTGATTATACCGAAGGAATA′] 1599 NM_000122.1(ERCC3): c.296T > 2071 ERCC3 [′CCAGTTTACAAATATGCCCAAGACTYCTTGG C (p.Phe99Ser) TGGCTATTGCAGAGCCAGTG′] 1600 NM_001113755.2(TYMP): c.854T > 1890 TYMP [′CTGGGTCGCTGCGTGGGCCACGCCCYGGAG C (p.Leu285Pro) GTGGAGGAGGCGCTGCTCTGC′] 1601 NM_000118.3(ENG): c.2T > C 2022 ENG [′CGCACAGGCCCCCACGTGGACAGCAYGGAC (p.Met1Thr) CGCGGCACGCTCCCTCTGGCT′] 1602 NM_203342.2(EPB41): c.2T > C 2035 EPB41 [′AAACCAATCAGAAAACACAGGAACABGCAC (p.Met1Thr) TGCAAGGTTTCTTTGTTGGAT′] 1603 NM_001972.2(ELANE): c.211T > 1991 ELANE [′CAACTTCGTCATGTCGGCCGCGCACYGCGTG C (p.Cys71Arg) GCGAATGTGTGAGTAGCCGG′] 1604 NM_000400.3(ERCC2): c.1454T > 2068 ERCC2 [′ACCATGGCAACCTTCACCATGACGCYGGCA C (p.Leu485Pro) CGGGTCTGCCTCTGCCCTATG′] 1605 NM_000281.3(PCBD1): c.244T > 5092 PCBD1 [′CCACATCACGCTGAGCACCCATGAGYGTGC C (p.Cys82Arg) CGGCCTTTCAGAACGGGACAT′] 1606 NM_001927.3(DES): c.1034T > C 1674 DES [′CCCCCTCTCCTGCAGAACGATTCCCYGATGA (p.Leu345Pro) GGCAGATGCGGGAATTGGAG′] 1607 NM_001927.3(DES): c.1154T > C 1674 DES [′CTCAAGGATGAGATGGCCCGCCATCYGCGC (p.Leu385Pro) GAGTACCAGGACCTGCTCAAC′] 1608 NM_000498.3(CYP11B2): c.1157T > −1 — [′GTTCTGAAGCACCAAGTCTGAGCTCRCCACT C (p.Val386Ala) CGCTCCAAAAACAGACCCAC′] 1609 NM_000498.3(CYP11B2): c.1382T > −1 — [′CGGCGCCTGGCAGAGGCAGAGATGCYGCTG C (p.Leu461Pro) CTGCTGCACCACGTAAGCAGG′] 1610 NM_001886.2(CRYBA4): c.281T > 1413 CRYBA4 [′TACCCCGCCGAGAGGCTCACCTCCTYCCGGC C (p.Phe94Ser) CTGCGGCCTGTGCTGTAAGT′] 1611 NM_001886.2(CRYBA4): c.206T > 1413 CRYBA4 [′GGCTTCCAAGGGCAGCAGTACATTCYGGAA C (p.Leu69Pro) CGAGGCGAATATCCAAGCTGG′] 1612 NM_000762.5(CYP2A6): c.670T > −1 — [′GCAACCCCAGCTCTATGAGATGTTCYCTTCG C (p.Ser224Pro) GTGATGAAACACCTGCCAGG′] 1613 NM_000165.4(GJA1): c.52T > C 2697 GJA1 [′ACTCCTTGACAAGGTTCAAGCCTACYCAACT (p.Ser18Pro) GCTGGAGGGAAGGTGTGGCT′] 1614 NM_000165.4(GJA1): c.32T > C 2697 GJA1 [′GACTGGAGCGCCTTAGGCAAACTCCYTGAC (p.Leu11Pro) AAGGTTCAAGCCTACTCAACT′] 1615 NM_004004.5(GJB2): c.101T > C 2706 GJB2 [′CTCCTTTGCAGCCACAACGAGGATCVTAATG (p.Met34Thr) CGAAAAATGAAGAGGACGGT′] 1616 NM_004004.5(GJB2): c.229T > C 2706 GJB2 [′CTTCCCCATCTCCCACATCCGGCTAYGGGCC (p.Trp77Arg) CTGCAGCTGATCTTCGTGTC′] 1617 NM_004004.5(GJB2): c.269T > C 2706 GJB2 [′ATCTTCGTGTCCACGCCAGCGCTCCYAGTGG (p.Leu90Pro) CCATGCACGTGGCCTACCGG′] 1618 NM_005215.3(DCC): c.503T > C 1630 DCC [′AAGTGTGAAGTCATTGGGGAGCCCAYGCCA (p.Met168Thr) ACAATCCACTGGCAGAAGAAC′] 1619 NM_001849.3(COL6A2): c.2329T > 1292 COL6A2 [′GAGTGAAAACCTCTACTCCATCGCCYGCGA C (p.Cys777Arg) CAAGCCACAGCAGGTGCGCAA′] 1620 NM_000090.3(COL3A1): c.582 + 1281 COL3A1 [′CTGGTCATCCTGGTTCCCCTGTAAGHATAGC 6T > C CATTGGTGGTGTTTTCTTCC′] 1621 NM_000089.3(COL1A2): c.279 + 1278 COL1A2 [′GTTGGACTTGGCCCTGGACCAATGGYATGCT 2T > C TATCTGTTTATCTTAGCCAA′] 1622 NM_000089.3(COL1A2): c.3105 + 1278 COL1A2 [′TTGCAAGGTCTGCCTGGTATCGCTGYAAGTA 2T > C AACTGTAGCCATCTCGCACA′] 1623 NM_000088.3(COL1A1): c.4391T > 1277 COL1A1 [′TTCGACGTTGGCCCTGTCTGCTTCCYGTAAA C (p.Leu1464Pro) CTCCCTCCATCCCAACCTGG′] 1624 NM_000493.3 (COL10A1): c.1841T > −1 — [′AAAGGGACTCATGTTTGGGTAGGCCYGTAT C (p.Leu614Pro) AAGAATGGCACCCCTGTAATG′] 1625 NM_000493.3(COL10A1): c.1771T > −1 — [′TGACCCAAGGACTGGAATCTTTACTYGTCAG C (p.Cys591Arg) ATACCAGGAATATACTATTT′] 1626 NM_000493.3(COL10A1): c.1951T > −1 — [′CGATCTCACAGAAAATGACCAGGTGYGGCT C (p.Trp651Arg) CCAGCTTCCCAATGCCGAGTC′] 1627 NM_000493.3(COL10A1): c.2011T > −1 — [′ATACTCCTCTGAGTATGTCCACTCCYCTTTCT C (p.Ser671Pro) CAGGATTCCTAGTGGCTCC′] 1628 NM_000493.3(COL10A1): c.1798T > −1 — [TCAGATACCAGGAATATACTATTTTYCATAC C (p.Ser600Pro) CACGTGCATGTGAAAGGGAC′] 1629 NM_020549.4(CHAT): c.629T > C 1103 CHAT [′ATGTATCTCAACAACCGCCTGGCCCYGCCTG (p.Leu210Pro) TCAACTCCAGCCCTGCCGTG′] 1630 NM_020549.4(CHAT): c.914T > C 1103 CHAT [′ATCATGCCGGAGCCTGAGCACGTCABCGTA (p.Ile305Thr) GCCTGCTGCAATCAGGTAAGC′] 1631 NM_020549.4(CHAT): c.1007T > 1103 CHAT [′GATCTGTTCACTCAGTTGAGAAAGAYAGTC C (p.Ile336Thr) AAAATGGCTTCCAACGAGGAC′] 1632 NM_001822.5(CHN1): c.427T > C 1123 CHN1 [′TGCCAAGATGACGATAAACCCAATTYATGA (p.Tyr143His) GCACGTAGGATACACAACCTT′] 1633 NM_001904.3(CTNNB1): c.133T > 1499 CTNNB1 [′TTCTGGTGCCACTACCACAGCTCCTBCTCTG C (p.Ser45Pro) AGTGGTAAAGGCAATCCTGA′] 1634 NM_004056.4(CA8): c.298T > C 767 CA8 [′ACATATATTTTCTTTTTCAGTTCTTYCGGGAG (p.Ser100Pro) GACCATTGCCTCAAGGGCA′] 1635 NM_016124.4(RHD): c.329T > C 6007 RHD [′CCTTCTGGGAAGGTGGTCATCACACYGTTCA (p.Leu110Pro) GGTATTGGGATGGTGGCTGG′] 1636 NM_015865.6(SLC14A1): c.871T > 6563 SLC14A1 [′CTTTGGACTCTGGGGTTTCAACAGCYCTCTG C (p.Ser291Pro) GCCTGCATTGCAATGGGAGG′] 1637 NM_001250.5(CD40): c.247T > C 958 CD40 [′GACACACTGCCACCAGCACAAATACYGCGA (p.Cys83Arg) CCCCAGTGCGTGCGCTGTTGG′] 1638 NM_000342.3(SLC4A1): c.2317T > 6521 SLC4A1 [′AGTGTGCGGCTCCCCCACAGGCCTGYCCATC C (p.Ser773Pro) CTCATGGAGCCCATCCTGTC′] 1639 NM_001681.3(ATP2A2): c.1678T > 488 ATP2A2 [′GGGTAGTGGCAGCGACACACTGCGAYGCCT C (p.Cys560Arg) GGCCCTGGCCACTCATGACAA′] 1640 NM_001082971.1(DDC): c.925T > 1644 DDC [TCCCCACAAATGGCTATTGGTGAATYTTGAC C (p.Phe309Leu) TGTTCTGCCATGTGGTAAGT′] 1641 NM_031226.2(CYP19A1): c.743 + −1 — [′ATACAAAAAGTATGAGAAGTCTGTGYAAGT 2T > C AATACAACTTTGGAAGATTTA′] 1642 NM_000486.5(AQP2): c.646T > C −1 — [′ACCCCTGGTGGGCGCCATCCTGGGCYCCCTC (p.Ser216Pro) CTCTACAACTACGTGCTGTT′] 1643 NM_000041.3(APOE): c.388T > C 348 APOE [′GCTGGGCGCGGACATGGAGGACGTGYGCGG (p.Cys130Arg) CCGCCTGGTGCAGTACCGCGG′] 1644 NM_000041.3(APOE): c.137T > C 348 APOE [′TGGCAGAGCGGCCAGCGCTGGGAACYGGCA (p.Leu46Pro) CTGGGTCGCTTTTGGGATTAC′] 1645 NM_000039.1(APOA1): c.220T > −1 — [′CTCCAGCCTAAAGCTCCTTGACAACYGGGA C (p.Trp74Arg) CAGCGTGACCTCCACCTTCAG′] 1646 NM_000039.1(APOA1): c.341T > −1 — [′CTGAGGCAGGAGATGAGCAAGGATCYGGAG C (p.Leu114Pro) GAGGTGAAGGCCAAGGTGCAG′] 1647 NM_000039.1(APOA1): c.593T > 335 APOA1 [′TACAGCGACGAGCTGCGCCAGCGCTYGGCC C (p.Leu198Ser) GCGCGCCTTGAGGCTCTCAAG′] 1648 NM_000416.2(IFNGR1): c.260T > 3459 IFNGR1 [′AATATTTCTCATCATTATTGTAATAYTTCTG C (p.Ile87Thr) ATCATGTTGGTGATCCATCA′] 1649 NM_000488.3(SERPINC1): c.1141T > 462 SERPINC1 [′TGTCGATCTGTTCAGCCCTGAAAAGYCCAAA C (p.Ser381Pro) CTCCCAGGTTTGTCTAGGAA′] 1650 NM_000488.3(SERPINC1): c.442T > 462 SERPINC1 [′GTTTGACACCATATCTGAGAAAACAYCTGAT C (p.Ser148Pro) CAGATCCACTTCTTCTTTGC′] 1651 NM_000488.3(SERPINC1): c.68T > 462 SERPINC1 [′AAGGTTTATCTTTTGTCCTTGCTGCYCATTG C (p.Leu23Pro) GCTTCTGGGACTGCGTGACC′] 1652 NM_000488.3(SERPINC1): c.667T > 462 SERPINC1 [′CAGAGCGGCCATCAACAAATGGGTGYCCAA C (p.Ser223Pro) TAAGACCGAAGGCCGAATCAC′] 1653 NM_000488.3(SERPINC1): c.379T > 462 SERPINC1 [′TTTTGCTATGACCAAGCTGGGTGCCYGTAAT C (p.Cys127Arg) GACACCCTCCAGCAACTGAT′] 1654 NM_001085.4(SERPINA3): c.233T > 12 SERPINA3 [′GATAAGAATGTCATCTTCTCCCCACYGAGCA C (p.Leu78Pro) TCTCCACCGCCTTGGCCTTC′] 1655 NM_000341.3(SLC3A1): c.1400T > 6519 SLC3A1 [′AATCAGTATGTCAACGTGATGAACAHGCTTC C (p.Met467Thr) TTTTCACACTCCCTGGAACT′] 1656 NM_000341.3(SLC3A1): c.2033T > −1 — [′AATCGAGCATGCTATTCCAGTGTACYGAAC C (p.Leu678Pro) ATACTGTATACCTCGTGTTAG′] 1657 NM_000021.3(PSEN1): c.749T > 5663 PSEN1 [′CCTGAATGGACTGCGTGGCTCATCTYGGCTG C (p.Leu250Ser) TGATTTCAGTATATGGTAAA′] 1658 NM_000021.3(PSEN1): c.338T > 5663 PSEN1 [′TTTTATACCCGGAAGGATGGGCAGCYGTAC C (p.Leu113Pro) GTATGAGTTTTGTTTTATTAT′] 1659 NM_000021.3(PSEN1): c.497T > 5663 PSEN1 [′CTTTTCTAGGTCATCCATGCCTGGCBTATTAT C (p.Leu166Pro) ATCATCTCTATTGTTGCTG′] 1660 NM_000021.3(PSEN1): c.254T > 5663 PSEN1 [′TATGGCGCCAAGCATGTGATCATGCYCTTTG C (p.Leu85Pro) TCCCTGTGACTCTCTGCATG′] 1661 NM_001151.3(SLC25A4): c.293T > 291 SLC25A4 [′GCCTTCAAGGACAAGTACAAGCAGCYCTTC T > C (p.Leu98Pro) TTAGGGGGTGTGGATCGGCAT′] 1662 NM_001100.3(ACTA1): c.287T > 58 ACTA1 [′TGGCACCACACCTTCTACAACGAGCYTCGCG C (p.Leu96Pro) TGGCTCCCGAGGAGCACCCC′] 1663 NM_001100.3(ACTA1): c.668T > 58 ACTA1 [′AAGGAGAAGCTGTGCTACGTGGCCCYGGAC C (p.Leu223Pro) TTCGAGAACGAGATGGCGACG′] 1664 NM_000485.2(APRT): c.407T > C 353 APRT [′CTCACCCTCCCATCCCCAGGAACCAYGAAC (p.Met136Thr) GCTGCCTGTGAGCTGCTGGGC′] 1665 NM_000485.2(APRT): c.329T > C 353 APRT [′CACCACTTCCCACTCCAGGCTGAGCYGGAG (p.Leu110Pro) ATTCAGAAAGACGCCCTGGAG′] 1666 NM_001614.3(ACTG1): c.1109T > 71 ACTG1 [′TACGACGAGTCGGGCCCCTCCATCGYCCACC C (p.Val370Ala) GCAAATGCTTCTAAACGGAC′] 1667 NM_000751.2(CHRND): c.283T > 1144 CHRND [′CCGGCTGAAGTGGAATGCTGAAGAAYTTGG C (p.Phe95Leu) AAACATCAGTGTCCTGCGCCT′] 1668 NM_000751.2(CHRND): c.188T > 1144 CHRND [′GCCCTGGCCCTCACACTCTCCAACCYCATCT C (p.Leu63Pro) CCCTGGTGAGAGGCCCTCCG′] 1669 NM_001039523.2(CHRNA1): c. 1134 CHRNA1 [′CTCTGTCTTACTGTCTTTGACTGTGYTCCTTC 901T > C (p.Phe301Leu) TGGTCATCGTGGAGCTGAT′] 1670 NM_001004127.2(AL G11): c.257T > 440138 ALG11 [′GGAGAAAGAGTTTTATGGTGTGCTTYAAGA C (p.Leu86Ser) GCCCTGCAGAAAAAGTAGGTA′] 1671 NM_002036.3(ACKR1): c.- 2532 ACKR1 [′AGAGGGAGCTAGGAGGCTAGCATAGRAAGG - 67T > C AGARAAGGAAAAAGACTTTGA′, 1674 ′TATAAAGACTGAGGGGCAAACAGCASGGGA AATGAGGGGCATAGGGATAAG′, ′CGCAGACAGAAGGGCTGGGACGGCTRTCAG CGCCTGTGCTTCCAAGRTAAG′, ′ATCAGGAAGCCTTACCCCACGCCCAYTGCCT GCACAAGCCTCAGGCCTATG′] 1675 NM_014053.3(FLVCR1): c.574T > 28982 FLVCR1 [′CAACTGCCTGGGTGCCTGGATCAAGYGCGG C (p.Cys192Arg) CAGTGTGCAGCAGCATCTCTT′] 1676 NM_004268.4(MED17): c.1112T > 9440 MED17 [′TGTCCGGAGGACCACCTTTATGTCCYAGAGC C (p.Leu371Pro) ATAATTTGCATCTACTGATT′] 1677 NM_024411.4(PDYN): c.632T > C 5173 PDYN [′CTGTACAAACGCTATGGGGGCTTCTYGCGGC (p.Leu211Ser) GCATTCGTCCCAAGCTCAAG′] 1678 NM_000018.3(ACADVL): c.848T > 37 ACADVL [′GTGAAGGAGAAGATCACAGCTTTTGYGGTG C (p.Val283Ala) GAGAGGGGCTTCGGGGGCATT′] 1679 NM_000021.3(PSEN1): c.1175T > 5663 PSEN1 [′GGAGATTTCATTTTCTACAGTGTTCYGGTTG C (p.Leu392Pro) GTAAAGCCTCAGCAACAGCC′] 1680 NM_000083.2(CLCN1): c.857T > 1180 CLCN1 [′CCACACTTCTGTGCCCCTGCAGGAGYGCTAT C (p.Val286Ala) TTAGCATCGAGGTCACCTCC′] 1681 NM_000083.2(CLCN1): c.920T > 1180 CLCN1 [′GTTCGGAACTACTGGAGAGGATTCTYTGCA C (p.Phe307Ser) GCCACGTTCAGCGCCTTTGTG′] 1682 NM_000157.3(GBA): c.703T > C 2629 GBA [′CAATGGAGCGGTGAATGGGAAGGGGYCACT (p.Ser235Pro) CAAGGGACAGCCCGGAGACAT′] 1683 NM_000166.5(GJB1): c.145T > C 2705 GJB1 [′AGAGAGTGTGTGGGGTGATGAGAAAYCTTC (p.Ser49Pro) CTTCATCTGCAACACACTCCA′] 1684 NM_000186.3(CFH): c.3590T > C 3075 CFH [′ATATCCCCGTTTACACACAAATTCARCTGAT - (p.Val1197Ala) TCACCTGTTCTCGAATAAAG′, 1686 ′GAACTTATCATTGCTGCTTTTGATTaagatat tggtcaaagagtacaaact′, ′ttcagctataatatgaataagaacttggaatc aaacatagagatctagtga] 1687 NM_000195.4(HPS1): c.2003T > 3257 HPS1 [′GGGGATGACAGACAGGTGCAGGGCCRGCAG C (p.Leu668Pro) CTCGTAGCACCTGACAGCCTC′] 1688 NM_000195.4(HPS1): c.716T > C 3257 HPS1 [′GCTGGGGTAGAGGTCCTGAACCAGGRGGAT (p.Leu239Pro) GAGGGCAAGCAGGTCGGCCGG′] 1689 NM_000334.4(SCN4A): c.4468T > 6329 SCN4A [′CCTCTTCAACATCGGCCTCCTCCTCYTCCTG C (p.Phe1490Leu) GTCATGTTCATCTACTCCAT′] 1690 NM_000352.4(ABCC8): c.257T > 6833 ABCC8 [′ACCTTCATGCTGCTCTTCGTCCTGGBGTGTG C (p.Val86Ala) AGATTGCAGAGGGCATCCTG′] 1691 NM_000352.4(ABCC8): c.404T > 6833 ABCC8 [′ATCGAGACTTCCAACTTCCCCAAGCYGCTAA C (p.Leu135Pro) TTGGTAGGTGAGGTGTAGGA′] 1692 NM_000352.4(ABCC8): c.674T > 6833 ABCC8 [′TTCCTGCAGCCCTTCGTGAATCTGCYGTCCA C (p.Leu225Pro) AAGGCACCTACTGGTGGATG′] 1693 NM_000430.3(PAFAH1B1): c.569 − 5048 PAFAH1B1 [′ATTACTTCATAATATATTGCTGTTAYGTGTTT 10T > C TAGGCCATGACCACAATGT′] 1694 NM_000525.3(KCNJ11): c.103T > 3767 KCNJ11 [′CCGTGCCCGCCAGCGGAGGGCCCGCBTTGT C (p.Phe35Leu) GTCCAAGAAAGGCAACTGCAA′] 1695 NM_000525.3(KCNJ11): c.755T > 3767 KCNJ11 [′GTGGGTGGCAACAGCATCTTCCTGGYGGCC C (p.Val252Ala) CCGCTGATCATCTACCATGTC′] 1696 NM_000528.3(MAN2B1): c.2426T > 4125 MAN2B1 [′GGCAGCAGCCTGAGAGATGGCTCGCYGGAG C (p.Leu809Pro) CTCATGGTGAGTGGGTCAGAG′] 1697 NM_001034850.2(FAM134B): c. 54463 FAM134B [′GACTTTTCAGCTCTTTGTCCTAAGGYATTTTT 873 + 2T > C TGTTTAGTTTTCAATTTGT′] 1698 NM_001457.3(FLNB): c.4804T > 2317 FLNB [′CTACGGGGGTGACGACATCCCACTTYCTCCT C (p.Ser1602Pro) TATCGCATCCGAGCCACACA′] 1699 NM_002863.4(PYGL): c.2461T > 5836 PYGL [′CTCCAGTGACCGAACAATTAAAGAAYATGC C (p.Tyr821His) CCAAAACATCTGGAACGTGGA′] 1700 NM_003002.3(SDHD): c.284T > C 6392 SDHD [′CCTTGCTCTGCGATGGACTATTCCCYGGCTG (p.Leu95Pro) CAGCCCTCACTCTTCATGGT′] 1701 NM_004385.4(VCAN): c.4004 − 1462 VCAN [′TAAGTATTGTGAAAACTCTGTTTTTHTCAGG 5T > C TCGAATGAGTGATTTGAGTG′] 1702 NM_004519.3(KCNQ3): c.925T > 3786 KCNQ3 [′TGAGACCTATGCAGATGCCCTGTGGYGGGG C (p.Trp309Arg) CCTGGTGAGTCACTACCTTGG′] 1703 NM_004937.2(CTNS): c.473T > C 1497 CTNS [′TCCACCCCCTGCAGTGTCATTGGTCYGAGCT (p.Leu158Pro) TCGACTTCGTGGCTCTGAAC′] 1704 NM_006329.3(FBLN5): c.649T > 10516 FBLN5 [′GAACGAGTGTGCCACCGAGAACCCCYGCGT C (p.Cys217Arg) GCAAACCTGCGTCAACACCTA′] 1705 NM_006432.3(NPC2): c.295T > C 10577 NPC2 [′TGATGGTTGTAAGAGTGGAATTAACYGCCCT (p.Cys99Arg) ATCCAAAAAGACAAGACCTA′] 1706 NM_007375.3(TARDBP): c.*83T > 23435 TARDBP [′CATGGTAAGTATATTGTAAAATACAYATGTA C CTAAGAATTTTCAAAATTGG′] 1707 NM_013246.2(CLCF1): c.46T > C −1 — [′CTCGTGGGGGATGTTAGCGTGCCTGYGCAC (p.Cys16Arg) GGTGCTCTGGCACCTCCCTGC′] 1708 NM_013246.2(CLCF1): c.676T > −1 — [′CCTGCACCTGGGGGCTCATGGCTTCYGACTT C (p.Ter226Arg) CTGACCTTCTCCTCTTCGCT′] 1709 NM_024577.3(SH3TC2): c.1982T > 79628 SH3TC2 [′CTGCCCTTTGCCGAGCGCCTGCAGCYCCTCT C (p.Leu661Pro) CTGGACACCCTCCTGCCTCT′] 1710 NM_172107.2(KCNQ2): c.2T > C 3785 KCNQ2 [′CCGGGGCGCCTCCCGCCAGGCACCAYGGTG (p.Met1Thr) CAGAAGTCGCGCAACGGCGGC′] 1711 NM_004304.4(ALK): c.3749T > C 238 ALK [′TGCATTTCCTTTCTTCCCAGAGACAYTGCTG (p.Ile1250Thr) CCAGAAACTGCCTCTTGACC′] 1712 NM_000495.4(COL4A5): c.4690T > 1287 COL4A5 [′TTCCTTCTCCTTTTCCTTTACCAGAYGTGCAG C (p.Cys1564Arg) TATGTGAAGCTCCAGCTGT′] 1713 NM_000495.4(COL4A5): c.4699T > 1287 COL4A5 [′CTTTTCCTTTACCAGATGTGCAGTAYGTGAA C (p.Cys1567Arg) GCTCCAGCTGTGGTGATCGC′] 1714 NM_000495.4(COL4A5): c.4756T > 1287 COL4A5 [′CAGTCAGACGATCCAGATTCCCCATYGTCCT C (p.Cys1586Arg) CAGGGATGGGATTCTCTGTG′] 1715 NM_000495.4(COL4A5): c.4803 + 1287 COL4A5 [′TAAGAAGCTTAAACTTCAAACAGCTYCTATC 121T > C CAAGCACTGTGTTCCCCCTC′] 1716 NM_000495.4(COL4A5): c.5032T > 1287 COL4A5 [′AGACTTGAGGACACGAATTAGCCGAYGTCA C (p.Cys1678Arg) AGTGTGCATGAAGAGGACATA′] 1717 NM_005359.5(SMAD4): c.970T > 4089 SMAD4 [′ATTTCCTATAGCTCCTGAGTATTGGYGTTCC C (p.Cys324Arg) ATTGCTTACTTTGAAATGGA′] 1718 NM_005359.5(SMAD4): c.1087T > 4089 SMAD4 [′GGACCCTTCTGGAGGAGATCGCTTTYGTTTG C (p.Cys363Arg) GGTCAACTCTCCAATGTCCA′] 1719 NM_005359.5(SMAD4): c.1598T > 4089 SMAD4 [′ATTGAAATTCACTTACACCGGGCCCBCCAGC C (p.Leu533Pro) TCCTAGACGAAGTACTTCAT′] 1720 NM_020630.4(RET): c.1831T > C 5979 RET [′TAAAGCTGGCTATGGCACCTGCAACNGCTTC (p.Cys611Arg) CCTGAGGAGGAGAAGTGCTT′] 1721 NM_020630.4(RET): c.1888T > C 5979 RET [′ACCACCCCCACCCACAGATCCACTGYGCGA (p.Cys630Arg) CGAGCTGTGCCGCACGGTGAT′] 1722 NM_000060.3(BTD): c.248T > C 686 BTD [′GCTCTCATCAGCCGCCAAGAGGCCTYGGAG (p.Leu83Ser) CTCATGAACCAGAACCTTGAC′] 1723 NM_000060.3(BTD): c.445T > C 686 BTD [′GAACCCATGCCTGGAGCCTCACCGCYTCAAT (p.Phe149Leu) GACACAGAGGTGATTCCTGC′] 1724 NM_000060.3(BTD): c.743T > C 686 BTD [′TTTGGCATCTTCACATGCTTTGATAYATTGTT (p.Ile248Thr) CTTTGACCCTGCCATCAGA′] 1725 NM_000060.3(BTD): c.764T > C 686 BTD [′GATATATTGTTCTTTGACCCTGCCAYCAGAG (p.Ile255Thr) TCCTCAGAGACTACAAGGTG′] 1726 NM_000060.3(BTD): c.833T > C 686 BTD [′ACTGCCTGGATGAACCAGCTCCCACYCTTGG (p.Leu278Pro) CAGCAATTGAGATTCAGAAA′] 1727 NM_000060.3(BTD): c.1096T > C 686 BTD [′CCATAGTAAGTTTTTAAAAATTTTGYCAGGC (p.Ser366Pro) GATCCGTACTGTGAGAAGGA′] 1728 NM_000060.3(BTD): c.1214T > C 686 BTD [′GAGATGATGTATGACAATTTCACCCYGGTCC (p.Leu405Pro) CTGTCTGGGGAAAGGAAGGC′] 1729 NM_000060.3(BTD): c.1252T > C 686 BTD [′GGGAAAGGAAGGCTATCTCCACGTCYGTTC (p.Cys418Arg) CAATGGCCTCTGCTGTTATTT′] 1730 NM_000060.3(BTD): c.1267T > C 686 BTD [′TCTCCACGTCTGTTCCAATGGCCTCYGCTGT (p.Cys423Arg) TATTTACTTTACGAGAGGCC′] 1731 NM_000060.3(BTD): c.1459T > C 686 BTD [′CACGGGGATATTTGAGTTTCACCTGYGGGGC (p.Trp487Arg) AACTTCAGTACTTCCTATAT′] 1732 NM_000155.3(GALT): c.265T > C 2592 GALT [′TCCTTGTCGGTAGGTGAATCCCCAGBACGAT (p.Tyr89His) AGCACCTTCCTGTTTGACAA′] 1733 NM_000155.3 (GALT): c.328 + 2T > 2592 GALT [′TGCAGCCTGATGCCCCCAGTCCAGGYAACCT C GGCTCCAACTGCTGCTGGGG′] 1734 NM_000155.3(GALT): c.336T > C 2592 GALT [′GATACTCCTTTACCTCAGGACCCAGYGATCA (p.Ser112=) TCCCCTTTTCCAAGCAAAGT′] 1735 NM_000155.3(GALT): c.350T > C 2592 GALT [TCAGGACCCAGTGATCATCCCCTTTYCCAAG (p.Phe117Ser) CAAAGTCTGCTCGAGGAGTC′] 1736 NM_000155.3(GALT): c.374T > C 2592 GALT [′TTCCAAGCAAAGTCTGCTCGAGGAGYCTGG (p.Val125Ala) TAACTATGGATTTCCCCTCTT′] 1737 NM_000155.3(GALT): c.425T > C 2592 GALT [′TGGTCGGATGTAACGCTGCCACTCAHGTCGG (p.Met142Thr) TCCCTGAGATCCGGGCTGTT′] 1738 NM_000155.3(GALT): c.452T > C 2592 GALT [′TCGGTCCCTGAGATCCGGGCTGTTGYTGATG (p.Val151Ala) CATGGGCCTCAGTCACAGAG′] 1739 NM_000155.3(GALT): c.460T > C 2592 GALT [′TGAGATCCGGGCTGTTGTTGATGCABGGGCC (p.Trp154Arg) TCAGTCACAGAGGAGCTGGG′] 1740 NM_000155.3(GALT): c.482T > C 2592 GALT [′GCATGGGCCTCAGTCACAGAGGAGCYGGGT (p.Leu161Pro) GCCCAGTACCCTTGGGTGCAG′] 1741 NM_000155.3(GALT): c.499T > C 2592 GALT [′AGAGGAGCTGGGTGCCCAGTACCCTYGGGT (p.Trp167Arg) GCAGGTTTGTGAGGTCGCCCC′] 1742 NM_000155.3 (GALT): c.507 + 2T > 2592 GALT [′GGTGCCCAGTACCCTTGGGTGCAGGYTTGTG C AGGTCGCCCCTTCCCCTGGA′] 1743 NM_000155.3(GALT): c.509T > C 2592 GALT [′GAGCTCCGTATCCCTATCTGATAGAHCTTTG (p.Ile170Thr) AAAACAAAGGTGCCATGATG′] 1744 NM_000155.3(GALT): c.584T > C 2592 GALT [′TGACAGGTATGGGCCAGCAGTTTCCYGCCA (p.Leu195Pro) GATATTGCCCAGCGTGAGGAG′] 1745 NM_000155.3(GALT): c.650T > C 2592 GALT [′TATAAGAGTCAGCATGGAGAGCCCCYGCTA (p.Leu217Pro) ATGGAGTACAGCCGCCAGGAG′] 1746 NM_000155.3(GALT): c.677T > C 2592 GALT [′CTAATGGAGTACAGCCGCCAGGAGCYACTC (p.Leu226Pro) AGGAAGGTGGGAGAGAGCCAA′] 1747 NM_000155.3(GALT): c.687 + 2T > 2592 GALT [′AGCCGCCAGGAGCTACTCAGGAAGGYGGGA C GAGAGCCAAGCCCTGTGTCCC′] 1748 NM_000155.3(GALT): c.745T > C 2592 GALT [′AGTACTGGTCCCCTTCTGGGCAACAYGGCCC (p.Trp249Arg) TACCAGACACTGCTGCTGCC′] 1749 NM_000155.3(GALT): c.967T > C 2592 GALT [′CCATTGGCAGCTGCACGCTCATTACBACCCT (p.Tyr323His) CCGCTCCTGCGCTCTGCCAC′] 1750 NM_000155.3(GALT): c.980T > C 2592 GALT [′CACGCTCATTACTACCCTCCGCTCCYGCGCT (p.Leu327Pro) CTGCCACTGTCCGGAAATTC′] 1751 NM_000155.3(GALT): c.1138T > 2592 GALT [′GGACAGGGAGACAGCAACCATCGCCYGACC C (p.Ter380Arg) ACGCCGACCACAGGGCCTTGA′] 1752 NM_207346.2(TSEN54): c.277T > 283989 TSEN54 [′AGAAGAGGGCTTCGTGGAGTTGAAGYCTCC C (p.Ser93Pro) CGCGGTGAGCGGCGGGCTCGG′] 1753 NM_016269.4(LEF1): c.181T > C −1 — [′CATCAAGTCTTCCTTGGTGAACGAGYCTGAA (p.Ser61Pro) ATCATCCCGGCCAGCAACGG′] 1754 NM_001928.2(CFD): c.640T > C 1675 CFD [′GGGTGACTCCGGGGGCCCGCTGGTGYGCGG (p.Cys214Arg) GGGCGTGCTCGAGGGCGTGGT′] 1755 NM_001458.4(FLNC): c.752T > C 2318 FLNC [′CCCAACGTGGATGAGCATTCTGTTAYGACCT (p.Met251Thr) ACCTGTCCCAGTTCCCCAAG′] 1756 NM_000781.2(CYP11A1): c.665T > 1583 CYP11A1 [′ATTTTTGGGGAGCGCCAGGGGATGCYGGAG C (p.Leu222Pro) GAAGTAGTGAACCCCGAGGCC′] 1757 NM_000782.4(CYP24A1): c.1226T > 1591 CYP24A1 [ATAATAATGATGGTTGccaacatgcgctgagt - C (p.Leu409Ser) gcttaaaatgtgccaggta′, 1761 ′ataaataggtaaagggctttagaattgtgcac catactcacaaccaccctg′, ′cgcagcagattctgttattaaacccchttaca gacgaagttgaggctcac′, ′agagattatgtaactcactcaagacaacacag ataaaagatgaaactgaat′, ′ACAAATTCTACTTACTCCTTTGGGTRAAGCAT ATTCACCCAGAACTGTTGC′] 1762 NM_001145661.1(GATA2): c.1117T > 2624 GATA2 [′CAACGGGGACCCTGTCTGCAACGCCYGTGG C (p.Cys373Arg) CCTCTACTACAAGCTGCACAA′] 1763 NM_004523.3(KIF11): c.2547 + 2T > 3832 KIF11 [′CAGGAACTTCACAACTTATTGGAGGYAATA C ACTTTGTAAGTGGAACTTACT′] 1764 NM_000238.3(KCNH2): c.1831T > 3757 KCNH2 [′GGGCGGCCCCTCCATCAAGGACAAGBATGT C (p.Tyr611His) GACGGCGCTCTACTTCACCTT′] 1765 NM_002465.3(MYBPC1): c.706T > 4604 MYBPC1 [′GGAGGAAGAACCCCAGGTGGACGTAYGGGA C (p.Trp236Arg) GTTGCTGAAGAACGCGAAACC′] 1766 NM_002465.3(MYBPC1): c.2566T > 4604 MYBPC1 [′CATTCCAAGACACCTGAAGCAAACCYATAT C (p.Tyr856His) CCGCAGAGTTGGAGAAGCTGT′] 1767 NM_005211.3(CSF1R): c.2624T > 1436 CSF1R [′AAACTGGTGAAGGATGGATACCAAAYGGCC C (p.Met875Thr) CAGCCTGCATTTGCCCCAAAG′] 1768 NM_005211.3(CSF1R): c.2381T > 1436 CSF1R [′TTGACCAATGGTCATGTGGCCAAGAYTGGG C (p.Ile794Thr) GACTTCGGGCTGGCTAGGGAC′] 1769 NM_003392.4(WNT5A): c.544T > 7474 WNT5A [′GCCGCGGGACTGGCTCTGGGGCGGCYGCGG C (p.Cys182Arg) CGACAACATCGACTATGGCTA′] 1770 NM_005188.3(CBL): c.1150T > C 867 CBL [′CTCCACATTCCAACTATGTAAAATABGTGCT (p.Cys384Arg) GAAAATGATAAGGATGTAAA′] 1771 NM_005188.3(CBL): c.1186T > C 867 CBL [′TGATAAGGATGTAAAGATTGAGCCCYGTGG (p.Cys396Arg) ACACCTCATGTGCACATCCTG′] 1772 NM_006902.4(PRRX1): c.338T > 5396 PRRX1 [′CAGCTGCAGGCTTTGGAGCGTGTCTYTGAGC C (p.Phe113Ser) GGACACACTATCCTGATGCT′] 1773 NM_001111035.1(ACP5): c.602T > −1 — [′GCGGCGGCCAGGGAGGACTACGTGCYGGTG C (p.Leu201Pro) GCTGGCCACTACCCCGTGTGG′] 1774 NM_000329.2(RPE65): c.1102T > 6121 RPE65 [′GGCTCCCCAACCTGAAGTTAGGAGAYATGT C (p.Tyr368His) ACTTCCTTTGAATATTGACAA′] 1775 NM_003172.3(SURF1): c.679T > 6834 SURF1 [′TGAGAACAATCCAGAAAGGAACCACYGGCA C (p.Trp227Arg) TTATCGAGACCTGGAAGCTAT′] 1776 NM_002734.4(PRKAR1A): c.1117T > 5573 PRKAR1A [′CATCCTCAAACGAAACATCCAGCAGYACAA C (p.Tyr373His) CAGTTTTGTGTCACTGTCTGT′] 1777 NM_002734.4(PRKAR1A): c.980T > 5573 PRKAR1A [′CCATCTTTGCTTTCTCCAGGTGAAAYTGCAC C (p.Ile327Thr) TACTGATGAATCGTCCTCGT′] 1778 NM_003491.3(NAA10): c.109T > 8260 NAA10 [GAAATACTACTTCTACCATGGCCTTYCCTGG C (p.Ser37Pro) CCCCAGGTGGGCAGCTTCTG′] 1779 NM_006306.3(SMC1A): c.2351T > 8243 SMC1A [′GTGTTTGAAGAGTTTTGTCGGGAGAYTGGTG C (p.Ile784Thr) TGCGCAACATCCGGGAGTTT′] 1780 NM_000377.2(WAS): c.814T > C 7454 WAS [′CGACCCAGATCTGCGGAGTCTGTTCYCCAGG (p.Ser272Pro) GCAGGAATCAGCGAGGCCCA′] 1781 NM_000377.2(WAS): c.881T > C 7454 WAS [′ACCTCTAAACTTATCTACGACTTCAYTGAGG (p.Ile294Thr) ACCAGGGTGGGCTGGAGGCT′] 1782 m.12338T > C 4540 MT-NDS [′TTGGTGCAACTCCAAATAAAAGTAAYAACC ATGCACACTACTATAACCACC′] 1783 m.5814T > C 4511 MT-TC [′TCGAATTTGCAATTCAATATGAAAAYCACCT CGGAGCTGGTAAAAAGAGGC′] 1784 m.12201T > C 4564 MT-TH [′GACAACAGAGGCTTACGACCCCTTAYTTACC GAGAAAGCTCACAAGAACTG′] 1785 NM_001165899.1(PDE4D): c.494T > 5144 PDE4D [′GGAGATGACTTGATTGTGACTCCATBTGCTC C (p.Phe165Ser) AGGTAAGCACAGCTTGGTGA′] 1786 NM_139125.3(MASP1): c.1888T > 5648 MASP1 [′GTTACCCGTGGTGCCTCACGCTGAGYGCAA C (p.Cys630Arg) AACTAGCTATGAGTCCCGCTC′] 1787 NM_007315.3(STAT1): c.520T > 6772 STAT1 [′TTTACAAGATGAATATGACTTCAAAYGCAA C (p.Cys174Arg) AACCTTGCAGAACAGAGGTAA′] 1788 NM_053025.3(MYLK): c.5275T > -1 — [′TGCTGTGAGAGCCATTGGAAGACTGYCCTCT C (p.Ser1759Pro) ATGGCAATGATCTCAGGGCT′] 1789 NM_001235.3(SERPINH1): c.233T > 871 SERPINH1 [′TCACCCGTGGTGGTGGCCTCGTCGCYAGGGC C (p.Leu78Pro) TCGTGTCGCTGGGCGGCAAG′] 1790 NM_005921.1(MAP3K1): c.566T > 4214 MAP3K1 [′GAGGAACGAATGATCAGGGAGAAACBGAA C (p.Leu189Pro) GGCAACCTGTATGCCAGCCTGG′] 1791 NM_005359.5(SMAD4): c.1499T > 4089 SMAD4 [′GTTGATGACCTTCGTCGCTTATGCAYACTCA C (p.Ile500Thr) GGATGAGTTTTGTGAAAGGC′] 1792 NM_000287.3(PEX6): c.1601T > 5190 PEX6 [′CTGTTGCTCACAGCTGTGGACCTTCYGGGCC C (p.Leu534Pro) GGGACCGTGATGGGCTGGGT′] 1793 NM_004153.3(ORC1): c.266T > C 4998 ORC1 [′AAGAAACGTGCTCGAGTACAGTGGTYTGTC (p.Phe89Ser) CGATTCTGTGAAGTCCCTGCC′] 1794 NM_006225.3(PLCD1): c.562T > 5333 PLCD1 [′CTCGGCTGTCCTGGCTCTGCAGGAGYGTGAC C (p.Cys188Arg) CACTCCCAGACAGACTCCCT′] 1795 NM_021252.4(RAB18): c.619T > 22931 RAB 18 [′CTGTGGTGGTTATTGCTCTGTGTTAYAAACT C (p.Ter207Gln) CTGGGAAATTCCATCTCTTG′] 1796 NM_002242.4(KCNJ13): c.722T > −1 — [′TGGTGTAATGGAGTGATAGTACGTTDGTGG C (p.Leu241Pro) AAAGATGAAGAATGGACATTC′] 1797 NM_003072.3(SMARCA4): c.3032T > 6597 SMARCA4 [′CTGCAGCGAGTGCTCTACCGCCACAYGCAG C (p.Met1011Thr) GCCAAGGGCGTGCTGCTGACT′] 1798 NM_013339.3 (AL G6): c.391T > C 29929 ALG6 [′GAGATTTCTTTTAAGCAACAACAGTRCAAAA (p.Tyr131His) CCACTGCAGGTATGTAAATC′] 1799 NM_019885.3(CYP26B1): c.436T > 56603 CYP26B1 [′TTGCCTCTCTGCCTTCCAGGTCTTCYCCAAG C (p.Ser146Pro) ATCTTCAGCCACGAGGCCCT′] 1800 NM_020433.4(JPH2): c.421T > C 57158 JPH2 [′GTTCACCAACGGCATGCGCCATGGCYACGG (p.Tyr141His) AGTACGCCAGAGCGTGCCCTA′] 1801 NM_000199.3(SGSH): c.892T > C 6448 SGSH [′CCCCAGCGTTTTGGGTGCTCCGGGGRTGACA (p.Ser298Pro) CCAGTAAGGGTTCAGCAGTG′] 1802 NM_173170.1(IL36RN): c.80T > 26525 IL36RN [′CTTTATCTGCATAATAACCAGCTTCYAGCTG C (p.Leu27Pro) GAGGGCTGCATGCAGGGAAG′] 1803 NM_016599.4(MYOZ2): c.142T > 51778 MYOZ2 [′CAGAGACATCATGTTGGAAGAATTAYCCCA C (p.Ser48Pro) TCTCAGTAACCGTGGTGCCAG′] 1804 NM_020191.2(MRPS22): c.644T > 56945 MRPS22 [′CCAATAATTTTCAAGGAAGAAAATCYTAGG C (p.Leu215Pro) GTAAGGTGACTTAGGTTTTAT′] 1805 NM_004278.3(PIGL): c.500T > C 9487 PIGL [′TTGTCCTATCCCTCCTCCAGGGCCCYGCACT (p.Leu167Pro) CAGAAGGGAAGTTACCTAAA′] 1806 NM_022445.3(TPK1): c.119T > C 27010 TPK1 [′ATTTTGTTCTTTCTATTTGAAGCTCYTTTAAG (p.Leu40Pro) AGCCTGTGCCGATGGAGGT′] 1807 NM_020634.1(GDF3): c.914T > C 9573 GDF3 [′TGTCCCTTCTCACTGACCATCTCTCYCAACA (pLeu305Pro) GCTCCAATTATGCTTTCATG′] 1808 NM_017882.2(CLN6): c.200T > C 54982 CLN6 [′CCCATTCTTCCATTTGCTCCGCAGCYGGTAT (p.Leu67Pro) TCCCTCTCGAGTGGTTTCCA′] 1809 NM_024513.3(FYC01): c.4127T > 79443 FYCO1 [′TTTGTGAGGTCCAGCACCTACAGCCYGATCC C (p.Leu1376Pro) CCATCACTGTGGCCGAGGCA′] 1810 NM_006147.3(IRF6): c.65T > C 3664 1RF6 [′CTGGTGGCCCAGGTGGATAGTGGCCYCTAC (p.Leu22Pro) CCTGGGCTCATCTGGCTACAC′] 1811 NM_025132.3(WDR19): c.2129T > 57728 WDR19 [′GGAAATGTTGGCATAGTGATGTCCTYGGAA C (p.Leu710Ser) CAAATAAAGGTAAACAGCATG′] 1812 NM_025132.3(WDR19): c.20T > 57728 WDR19 [′TTTATTTTTTAGCGTATTTTCTCACYGCTAGA C (p.Leu7Pro) AAAGACTTGGCTTGGCGCA′] 1813 NM_153026.2(PRICKLE1): c.1414T > 144165 PRICKLE1 [′AAGTAAAAAATACCAGTCTGATATGYACTG C (p.Tyr472His) GGCACAGTCACAAGATGGACT′] 1814 NM_014874.3(MFN2): c.1392 + 9927 MFN2 [′GTAGTCCTCAAGGTTTATAAGAATGWGAGT 2T > C CATGGAGCAACAGGTCCTCTT′] 1815 NM_014874.3(MFN2): c.647T > C 9927 MFN2 [′GAGCTGGACAGCTGGATTGACAAGTYTTGT (p.Phe216Ser) CTGGATGCTGATGTGTTTGTG′] 1816 NM_016097.4(IER31P1): c.233T > 51124 IER3IP1 [′GTAAACTCAATTGCAATTGTGTTACYTTTAT C (p.Leu78Pro) TATTTGGATGAATATCAGTG′] 1817 NM_018718.2(CEP41): c.107T > 95681 CEP41 [′TTCGAGCTTCTCAGTATATTTAGTCRTACTGT C (p.Met36Thr) TACCTAAAAAGAAAATAAG′] 1818 NM_022489.3(INF2): c.310T > C 64423 INF2 [′CCTGCTGCAGCTCACCTGCGTCAGCYGCGTG (p.Cys104Arg) CGCGCCGTCATGAACTCGCG′] 1819 NM_022489.3(INF2): c.383T > C 64423 INF2 [′AGCAACCAGGGCTACGTGCGCCAGCYCTCC (p.Leu128Pro) CAGGGTGAGCCGCAGTGTGGG′] 1820 NM_018713.2(SLC30A10): c.266T > 55532 SLC30A10 [′GGCGCGCTGAGCAACGCGGTCTTCCYCACC C (p.Leu89Pro) GCGCTCTGCTTCACCATCTTC′] 1821 NM_058246.3(DNAJB6): c.277T > 10049 DNAJB6 [′CAGTCCATTTGAATTTGGCTTCACAYTCCGT C (p.Phe93Leu) AACCCAGATGATGTCTTCAG′] 1822 NM_024753.4(TTC21B): c.2384T > 79809 TTC21B [′CAAAAGAATTATCTTTGCTATGACCYGGCTG C (p.Leu795Pro) AGCTCTTATTAAAATTGAAA′] 1823 NM_032446.2(MEGF10): c.2320T > 84466 MEGF10 [′TGACTGCGACCACATTTCTGGGCAGYGTACT C (p.Cys774Arg) TGCCGCACTGGATTCATGGG′] 1824 NM_032446.2(MEGF10): c.976T > 84466 MEGF10 [′CGTTCTCTGTGCTGAGACCTGCCAGYGTGTC C (p.Cys326Arg) AACGGAGGGAAGTGTTACCA′] 1825 NM_024027.4(COLEC11): c.505T > 78989 COLEC11 [′GAAGCGCTACGCGGACGCCCAGCTGYCCTG C (p.Ser169Pro) CCAGGGCCGCGGGGGCACGCT′] 1826 NM_000035.3(ALDOB): c.1027T > 229 ALDOB [′GCCCCAGAAGAACCCGTGTGAACATRCTGT C (p.Tyr343His) CCTTTGGCCGCCTGGCAGTTA′] 1827 NM_012338.3(TSPAN12): c.734T > 23554 TSPAN12 [′CCTTCTATCATAATACAGAGCCCAGRGCAGA C (p.Leu245Pro) GTAATGGTGAGAATCATGGC′] 1828 NM_001159772.1(CANT1): c.671T > 124583 CANT1 [′CTCCTTGCCCAGGCCGCCCACGTACRGACGC C (p.Leu224Pro) TCGTCCTTCACTGCCAGCCA′] 1829 NM_001006657.1(WDR35): c.781T > 57539 WDR35 [′TGGCATGTACGTAGTAGGCATCCAGYGGAA C (p.Trp261Arg) CCACATGGGCAGCGTGTTAGC′] 1830 NM_000420.2(KEL): c.1790T > C 3792 KEL [′TCAACAGTACTGCCTGGGGGCTGCCYCGCCT (p.Leu597Pro) GTGACAACCATGCCCTCCAG′] 1831 NM_001243473.1(B9D1): c.400 + 27077 B9D1 [′AGGGCCCAGGTCAGAATGAGGACCTRCCGG 2T > C CCAGGTGAGAAGGGCACGTGC′] 1832 NM_015175.2(NBEAL2): c.1163T > 23218 NBEAL2 [′ATTGCAGTCCATGTAGTCAGAGTGCYGACCT C (p.Leu388Pro) GCATCATGAGTGACTCCCCC′] 1833 NM_000196.3(HSD11B2): c.1012T > 3291 HSD11B2 [′GCTGGCAGCTCGGCCCCGCCGCCGCYATTAC C (p.Tyr338His) CCCGGCCAGGGCCTGGGGCT′] 1834 NM_024599.5(RHBDF2): c.557T > 79651 RHBDF2 [′GCTTACCGCCCCCCTCCCTTCCAGAYTGTGG C (p.Ile186Thr) ATCCGCTGGCCCGGGGCCGG′] 1835 NM_001256714.1(DNAAF3): c. 352909 DNAAF3 [′GGCTCTGTGGATGGACGGCACCTGCYGCGG 386T > C (p.Leu129Pro) ACCCTGTCCCGAGCGAAGTTC′] 1836 NM_020894.2(UVSSA): c.94T > C 57654 UVSSA [′GAAAATGAAGGAACTGAAGAAAATTYGCAA (p.Cys32Arg) GTATGTCTTAGGGTTCAGTAA] 1837 NM_004453.3(ETFDH): c.1130T > 2110 ETFDH [′TTGTTTCCTCAGTCTATACCAAAACYCACCT C (p.Leu377Pro) TTCCTGGTGGTTTACTAATT′] 1838 NM_024306.4(FA2H): c.707T > C 79152 FA2H [′CTCATCGAGTACCTCATCCACCGCTYCCTGT (p.Phe236Ser) TCCACATGAAGCCCCCCAGC′] 1839 NM_001004127.2(ALG11): c.1142T > −1 — [′AAAATAAACATTCCATTTGATGAATYAAAG C (p.Leu381Ser) AATTATTTGTCTGAAGCAACA] 1840 NM_021167.4(GATAD1): c.304T > 57798 GATAD1 [′TCGGCTCAGAAACACTAAATACAAAYCTGC C (p.Ser102Pro) TCCGGCTGCTGAAAAGAAAGT′] 1841 NM_016042.3(EXOSC3): c.712T > 51010 EXOSC3 [′AGTATTTGGAATGAATGGAAGAATAYGGGT C (p.Trp238Arg) TAAGGCAAAAACCATCCAGCA′] 1842 NM_001018005.1(TPM1): c.515T > 7168 TPM1 [′CAGGTGGCCCGTAAGCTGGTCATCAYTGAG C (p.Ile172Thr) AGCGACCTGGAACGTGCAGAG′] 1843 NM_001018005.1(TPM1): c.842T > 7168 TPM1 [′GAGCTGGACCACGCTCTCAACGATAYGACT C (p.Met281Thr) TCCATGTAAACGTTCATCCAC′] 1844 NM_033360.3(KRAS): c.211T > C 3845 KRAS [′GGAGTACAGTGCAATGAGGGACCAGYACAT (p.Tyr71His) GAGGACTGGGGAGGGCTTTCT′] 1845 NM_006218.2(PEK3CA): c.1258T > 5290 PIK3CA [′TTTTTCTTTGTTTTTTAAGGAACACYGTCCAT C (p.Cys420Arg) TGGCATGGGGAAATATAAA′] 1846 NM_006265.2(RAD21): c.1753T > −1 — [′TGAATCTATCAGTTTGCTTGAGTTAYGTCGA C (p.Cys585Arg) AATACGAACAGAAAACAAGC′] 1847 NM_000222.2(KIT): c.1859T > C 3815 KIT [′ATTAAGTCAGATGCGGCCATGACTGYCGCT (p.Val620Ala) GTAAAGATGCTCAAGCGTAAG′] 1848 NM_000076.2(CDKN1C): c.827T > 1028 CDKN1C [′CGCGCGCTGTCGCCCGCAGATTTCTYCGCCA C (p.Phe276Ser) AGCGCAAGAGATCAGCGCCT′] 1849 NM_005691.3(ABCC9): c.3058T > 10060 ABCC9 [′AGACTATTGGCTGGCCACATGGACAYCGGA C (p.Ser1020Pro) GTACAGTATAAACAATACTGG′] 1850 NM_012343.3(NNT): c.2930T > C 23530 NNT [′ATGCCTGGTCAGCTTAATGTGCTGCYGGCTG (p.Leu977Pro) AGGCTGGTGTGCCATATGAC′] 1851 NM_024110.4(CARD14): c.467T > 79092 CARD14 [′GTGCTGCTGCGGCGGTGCCAGCAGCYGCAG C (p.Leu156Pro) GAGCACCTGGGCCTGGCCGAG′] 1852 NM_000492.3(CFTR): c.1400T > 1080 CFTR [′GATGGGTTTTATTTCCAGACTTCACYTCTAA C (p.Leu467Pro) TGGTGATTATGGGAGAACTG′] 1853 NM_000518.4(HBB): c.2T > C 3043 HBB [′CACTAGCAACCTCAAACAGACACCANGGTG (p.Met1Thr) CATCTGACTCCTGAGGAGAAG′] 1854 NM_000518.4(HBB): c.*110T > C 3043 HBB [′CCTTGAGCATCTGGATTCTGCCTAAHAAAAA ACATTTATTTTCATTGCAAT′] 1855 NM_000518.4(HBB): c.92 + 2T > C 3043 HBB [′AGTTGGTGGTGAGGCCCTGGGCAGGNTGGT ATCAAGGTTACAAGACAGGTT′] 1856 NM_000206.2(IL2RG): c.455T > 3561 IL2RG [′ACCACATATGCACACATATCTCCAGYGATCC C (p.Val152Ala) CCTGGGCTCCAGAGAACCTA′] 1857 NM_000244.3(MEN1): c.518T > C 4221 MEN1 [′GCTGTGGTTGGGGCCTGCCAGGCCCYGGGT (p.Leu173Pro) CTCCGGGATGTCCACCTCGCC′] 1858 NM_000256.3(MYBPC3): c.2374T > 4607 MYBPC3 [′CCGCCATCGTAGGCAGGCGGCTCCCVCTGTA C (p.Trp792Arg) CTGTGCAGGAGTCCTCTCCC′] 1859 NM_002501.3(NFIX): c.179T > C 4784 NFIX [′GAGGAGCGGGCGGTGAAGGACGAGCYGCTG (p.Leu60Pro) GGCGAGAAGCCCGAGATCAAG′] 1860 NM_005022.3(PFN1): c.341T > C 5216 PFN1 [′CCTCCTCCAGCGCTAGTCCTGCTGAYGGGCA (p.Met114Thr) AAGAAGGTGTCCACGGTGGT′] 1861 NM_001172567.1(MYD88): c.818T > 4615 MYD88 [′CTTGCAGGTGCCCATCAGAAGCGACYGATC C (p.Leu273Pro) CCCATCAAGTACAAGGCAATG′] 1862 NM_001161581.1(POC1A): c.398T > 25886 POC1A [′GCCAGTGATGACAAGACTGTTAAGCYGTGG C (p.Leu133Pro) GACAAGAGCAGCCGGGAATGT′] 1863 NM_005270.4(GLI2): c.4663T > C 2736 GLI2 [′CCCCCGAAACTCCTTGACCCTGCCCYCCATC (p.Ser1555Pro) CCCGCAGGCATCAGCAACAT′] 1864 NM_152296.4(ATP1A3): c.2431T > 478 ATP1A3 [′CTCCTCCGCCTAGGTCCCTGCCATCYCACTG C (p.Ser811Pro) GCGTACGAGGCTGCCGAAAG′] 1865 NM_022787.3(NMNAT1): c.838T > 64802 NMNAT1 [′GAGAAACACTGCAGAAGCTAAGACAYAGGA C (p.Ter280Gln) ATTCTACAGCATGATATTTCA′] 1866 NM_005340.6(HINT1): c.250T > 3094 HINT1 [′ACACTTAATGATTGTTGGCAAGAAAYGTGCT C (p.Cys84Arg) GCTGATCTGGGCCTGAATAA′] 1867 NM_000155.3(GALT): c.416T > C 2592 GALT [′TTCCACCCCTGGTCGGATGTAACGCYGCCAC (p.Leu139Pro) TCATGTCGGTCCCTGAGATC′] 1868 NM_000155.3(GALT): c.386T > C 2592 GALT [′CTCCCGTCACCACCCAGTAAGGTCAYGTGCT (p.Met129Thr) TCCACCCCTGGTCGGATGTA′] 1869 NM_000155.3(GALT): c.680T > C 2592 GALT [′ATGGAGTACAGCCGCCAGGAGCTACYCAGG (p.Leu227Pro) AAGGTGGGAGAGAGCCAAGCC′] 1870 NM_007294.3(BRCA1): c.4986 + 672 BRCA1 [′GCCTGACCCCAGAAGAATTTGTGAGBGTATC 6T > C CATATGTATCTCCCTAATGA′] 1871 NM_007294.3(BRCA1): c.5074 + 672 BRCA1 [′CTACTCATGTTGTTATGAAAACAGGYATACC 2T > C AAGAACCTTTACAGAATACC′] 1872 NM_007294.3(BRCA1): c.5207T > 672 BRCA1 [′GGTTTCTTTCAGCATGATTTTGAAGBCAGAG C (p.Val1736Ala) GAGATGTGGTCAATGGAAGA′] 1873 NM_002437.4(MPV17): c.186 + 4358 MPV17 [′TGAAGCCCTGTTGAGGGGAGAACTTRCCAC 2T > C AAAGCCACAGCCCAGGGACAC′] 1874 NM_002769.4(PRSS1): c.116T > −1 — [′GAGGAGAATTCTGTCCCCTACCAGGYGTCCC C (p.Val39Ala) TGAATTCTGGCTACCACTTC′] 1875 NM_004211.3(SLC6A5): c.1444T > 9152 SLC6A5 [′GATTTTCTTCTCTTTATCTGCTGCAYGGGGA C (p.Trp82Arg) GGCCTGATCACTCTCTCTTC′] 1876 NM_005211.3(CSF1R): c.2297T > 1436 CSF1R [′TTCTCCAGCCAAGTAGCCCAGGGCAYGGCC C (p.Met766Thr) TTCCTCGCTTCCAAGAATGTG′] 1877 NM_005211.3(CSF1R): c.2546T > 1436 CSF1R [TATGGCATCCTCCTCTGGGAGATCTYCTCAC C (p.Phe849Ser) TTGGTGAGCCACTGGGCCCA′] 1878 NM_005211.3(CSF1R): c.2603T > 1436 CSF1R [′CTGGTGAACAGCAAGTTCTATAAACBGGTG C (p.Leu868Pro) AAGGATGGATACCAAATGGCC′] 1879 NM_006796.2(AFG3L2): c.1997T > 10939 AFG3L2 [′ATTTTTCAGATTGTTCAGTTTGGCABGAATG C (p.Met666Thr) AAAAGGTTGGGCAAATCTCC′] 1880 NM_018713.2(SLC30A10): c.1046T > 55532 SLC30A10 [′GTAAGTGGAAAGATTATTGCCACCCYGCAC C (p.Leu349Pro) ATCAAGTATCCTAAGGACAGG′] 1881 NM_018713.2(SLC30A10): c.500T > 55532 SLC30A10 [′GCGGAGGGCTGTGTCCCCGGCGCTTYCGGG C (p.Phe167Ser) GGGCCTCAGGGCGCGGAGGAC′] 1882 NM_000060.3(BTD): c.212T > C 686 BTD [′TATGAGCATCCATCCATCCTGAGTCYGAACC (p.Leu71Pro) CTCTGGCTCTCATCAGCCGC′] 1883 NM_000495.4(COL4A5): c.438 + 1287 COL4A5 [′TTTCCTGGTTTACAGGGTCCTCCAGYAAGTT 2T > C ATAAAATTTGGGATTATGAT′] 1884 NM_000282.3(PCCA): c.491T > C 5095 PCCA [′CAGGCAGCAGAAGATGTCGTTTTCAYTGGA (p.Ile164Thr) CCTGACACACATGCTATTCAA′] 1885 NM_000532.4(PCCB): c.1556T > 5096 PCCB [′ACACGTGCCCGAATCTGCTGTGACCYGGAT C (p.Leu519Pro) GTCTTGGCCAGCAAGAAGGTA′] 1886 NM_001099274.1(TINF2): c.860T > 26277 TINF2 [′CATAAGGAGCGCCCCACAGTCATGCYGTTTC C (p.Leu287Pro) CCTTTAGGAATCTCGGCTCA′] 1887 NM_001099274.1(TINF2): c.862T > 26277 TINF2 [′TAAGGAGCGCCCCACAGTCATGCTGYTTCCC C (p.Phe288Leu) TTTAGGAATCTCGGCTCACC′] 1888 NM_001363.4(DKC1): c.1049T > 1736 DKC1 [′TCAATGCCTGTAGCTATTGCATTAAYGACCA C (p.Met350Thr) CAGCGGTCATCTCTACCTGC′] 1889 NM_001363.4(DKC1): c.1193T > 1736 DKC1 [′AAGCTGATGATCAAGCAGGGCCTTCYGGAC C (p.Leu398Pro) AAGCATGGGAAGCCCACAGAC′] 1890 NM_004614.4(TK2): c.156 + 2T > 7084 TK2 [′CAGGAAAAAGAGAAAAAATCAGTGGYAAG C TCCCTCTTTTATGTGTACTCTC′] 1891 NM_004614.4(TK2): c.644T > C 7084 TK2 [′GAATACCTGGAAGCAATTCACCATCYCCAT (p.Leu215Pro) GAGGAGTGGCTCATCAAAGGC′] 1892 NM_024312.4(GNPTAB): c.1208T > 79158 GNPTAB [′ATCGAAGGGCTGTCCCAGAAGTTTAYTTACC C (p.Ile403Thr) TAAATGATGATGTCATGTTT′] 1893 NM_024312.4(GNPTAB): c.3002T > 79158 GNPTAB [′TTTGCCTTCTCTTATTTTTATTATCYCATGAG C (p.Leu1001Pro) TGCAGTGCAGCCACTGAAT′] 1894 NM_198253.2(TERT): c.3043T > 7015 TERT [′CCGCCATCCTCTCAGGTTTCACGCAYGTGTG C (p.Cys1015Arg) CTGCAGCTCCCATTTCATCA′] 1895 NM_207352.3(CYP4V2): c.1021T > 285440 CYP4V2 [′TACAACTGCAGCTGCAATAAACTGGYCCTTA C (p.Ser341Pro) TACCTGTTGGGTTCTAACCC′] 1896 NM_207352.3(CYP4V2): c.655T > 285440 CYP4V2 [′TGCTCAAAGTAATGATGATTCCGAGYATGTC C (p.Tyr219His) CGTGCAGTTTATAGGTAAAT′] 1897 NM_000335.4(SCN5A): c.5504T > 6331 SCN5A [′GCCAAGCCCAACCAGATAAGCCTCAYCAAC C (p.Ile1835Thr) ATGGACCTGCCCATGGTGAGT′] 1898 NM_001111.4(ADAR): c.2615T > 103 ADAR [′CTCGGCCGCAAGATTCTGGCCGCCAYCATTA C (p.Ile872Thr) TGAAAAAAGACTCTGAGGAC′] 1899 NM_004281.3(BAG3): c.1385T > 9531 BAG3 [′ATCGAAGAGTATTTGACCAAAGAGCYGCTG C (p.Leu462Pro) GCCCTGGATTCAGTGGACCCC′] 1900 NM_183075.2(CYP2U1): c.784T > 113612 CYP2U1 [′TTTTATGTCACGAGGCCTAGAAATCYGTCTG C (p.Cys262Arg) AACAGTCAAGTCCTCCTGGT′] 1901 NM_006177.3(NRL): c.287T > C 4901 NRL [′TTGGGGCTGAGTCCTGAAGAGGCCAYGGAG (p.Met96Thr) CTGCTGCAGGGTCAGGGCCCA′] 1902 NM_000344.3(SMN1): c.388T > C 6606 SMN1 [′AACCTGTGTTGTGGTTTACACTGGAYATGGA (p.Tyr130His) AATAGAGAGGAGCAAAATCT′] 1903 NM_152692.4(C1GALT1C1): c. 29071 C1GALT1C1 [′AGGAGGAATTGTCTTAAGTGTAGAAYCAAT 577T > C (p.Ser193Pro) GAAAAGACTTAACAGCCTTCT′] 1904 NM_024531.4(SLC52A2): c.368T > 79581 SLC52A2 [′TTAGCACTGGCCTTTGTGCTGGCACYGGCAT C (p.Leu123Pro) GCTGTGCCTCGAATGTCACT′] 1905 NM_001253816.1(SLC52A2): c. 79581 SLC52A2 [′GCTCACTGCAGGTCCTTGGCAGGGCYGGGC 1016T > C (p.Leu339Pro) GGCCTCTCTCTGCTGGGCGTG′] 1906 NM_002609.3(PDGFRB): c.1973T > 5159 PDGFRB [′TCGGAGCTGAAGATCATGAGTCACCYTGGG C (p.Leu658Pro) CCCCACCTGAACGTGGTCAAC′] 1907 NM_020822.2(KCNT1): c.2386T > 57582 KCNT1 [′CAACAGCTATGAAGACGCCAAGGCCYACGG C (p.Tyr796His) GTTCAAGAACAAGCTGATCAT′] 1908 NM_001369.2(DNAH5): c.1121T > 1767 DNAH5 [′ACTATAGATCATTTTAATTGCATTTRTAAGT C (p.Ile374Thr) GTAGGAATAGCATCCATCAT′] 1909 NM_017802.3(DNAAF5): c.2384T > 54919 DNAAF5 [′GTCCAGTACCTGTACCGAGAGTTGCYGGTTC C (p.Leu795Pro) ACCTTGACGATCCAGAGAGG′] 1910 NM_006383.3(CIB2): c.272T > C 10518 C1B2 [′GAGGGGAACCTCACTTTCAACGACTYTGTG (p.Phe91Ser) GACATGTTTTCCGTGCTCTGC′] 1911 NM_006383.3(CIB2): c.368T > C 10518 C1B2 [′ACAGACTTCAACACTGACAACTTCAYCTGCA (p.Ile123Thr) AGGAGGACCTGGAGCTGACG′] 1912 NM_005334.2(HCFC1): c.- 3054 HCFC1 [′TTAGTTGTTACTTCTTCACACAAGAYGGCGG 970T > C CTCCCAGGGAGGAGGCATGA′] 1913 NM_178012.4(TUBB2B): c.350T > 347733 TUBB2B [′GGAGCCGAGCTGGTCGACTCGGTCCYGGAT C (p.Leu117Pro) GTGGTGAGGAAGGAGTCAGAG′] 1914 NM_000431.3(MVK): c.764T > C 4598 MVK [′GTGGCTGGCGTCAGAAACAGGCTGCYCAAG (p.Leu255Pro) GTGACTCTTGTTCCCTTCTTG′] 1915 NM_000431.3(MVK): c.122T > C 4598 MVK [′TTGAACTTGAGAACATTCCTCCGGCYTCAAC (p.Leu41Pro) CCCACAGCAATGGGAAAGTG′] 1916 NM_000431.3(MVK): c.1039 + 2T > 4598 MVK [′GTGGCATCACACTCCTCAAGCCAGGYATCCC C GGGGGTAGGTGGGCCAGGCT′] 1917 NM_000431.3(MVK): c.1094T > 4598 MVK [′AAGCAGGCCCTGACCAGCTGTGGCTYTGAC C (p.Phe365Ser) TGCTTGGAAACCAGCATCGGT′] 1918 NM_004055.4(CAPN5): c.731T > 726 CAPN5 [′ACAGCAGCTGACATGGAGGCCCGCCYGGCG C (p.Leu244Pro) TGCGGCCTGGTAAAGGGCCAC′] 1919 NM_133497.3(KCNV2): c.491T > 169522 KCNV2 [GCCGTCTTCCAGCTGGTCTACAATTYCTACC C (p.Phe164Ser) TGTCCGGGGTGCTGCTGGTG′] 1920 NM_006567.3(FARS2): c.986T > 10667 FARS2 [′ATGATCCTCTACGACATCCCTGATAYCCGTC C (p.Ile329Thr) TCTTCTGGTGTGAGGACGAG′] 1921 NM_020376.3(PNPLA2): c.757 + 57104 PNPLA2 [′GCCTGCGCTTTCTGCAGCGGAACGGYGCGC 2T > C GGACCCGGGCGGGAGAGGGCG′] 1922 NM_018344.5(SLC29A3): c.607T > 55315 SLC29A3 [′TATGAGGAACTCCCAGGCACTGATAYCAGG C (p.Ser203Pro) TGAGAGCCAGGGTCCGGGCAG′] 1923 NM_012275.2(IL36RN): c.115 + 26525 IL36RN [′TGCAGGGAAGGTCATTAAAGGTTGGYGATG 6T > C AAACATGACCCACTTTCCTTG′] 1924 m.9191T > C 4508 MT-ATP6 [′TTCACACTTCTAGTAAGCCTCTACCYGCACG ACAACACATAATGACCCACC′] 1925 NM_000108.4(DLD): c.140T > C 1738 DLD [′GTAGTTGATGCTGATGTAACAGTTAYAGGTT (p.Ile47Thr) CTGGTCCTGGAGGATATGTT′] 1926 NM_000080.3(CHRNE): c.223T > −1 — [′AGAGGAGACTCTCACCACTAGCGTCYGGAT C (p.Trp75Arg) TGGAATCGTGAGTCAAATCTG′] 1927 NM_004376.5(COX15): c.1030T > 1355 COX15 [′TGCCATTACAGTGCTCTACTTCCTCBCTCGG C (p.Ser344Pro) AGAATTCCCCTTCCTAGAAG′] 1928 NM_004333.4(BRAF): c.1403T > 673 BRAF [′GGACAAAGAATTGGATCTGGATCATYTGGA C (p.Phe468Ser) ACAGTCTACAAGGGAAAGTGG′] 1929 NM_004333.4(BRAF): c.1454T > 673 BRAF [TTAGGTGATGTGGCAGTGAAAATGTYGAAT C (p.Leu485Ser) GTGACAGCACCTACACCTCAG′] 1930 NM_005188.3(CBL): c.1201T > C 867 CBL [′GATTGAGCCCTGTGGACACCTCATGYGCAC (p.Cys401Arg) ATCCTGTCTTACATCCTGGCA′] 1931 NM_002834.3(PTPN11): c.211T > 5781 PTPN11 [′CTATGACCTGTATGGAGGGGAGAAABTTGC C (p.Phe71Leu) CACTTTGGCTGAGTTGGTCCA′] 1932 NM_002834.3(PTPN11): c.853T > 5781 PTPN11 [′AAATAGATATAAAAACATCCTGCCCBGTAA C (p.Phe285Leu) GTATCAATATTCCGCTCAGTA′] 1933 NM_002880.3(RAF1): c.769T > C 5894 RAF1 [′AGGTTCCCTCTCCCAGAGGCAGAGGYCGAC (p.Ser257Pro) ATCCACACCTAATGTCCACAT′] 1934 NM_002880.3(RAF1): c.1423T > 5894 RAF1 [′AGTCCTTAACAAGCATTGAGATATAYTTCTC C (p.Phe475Leu) CATGAAGGCTTAACAGTGAA′] 1935 NM_005633.3(SOS1): c.806T > C 6654 SOS1 [′GGCCATATAGAAGATACAGTAGAAABGACA (p.Met269Thr) GATGAAGGCAGTCCCCATCCA′] 1936 NM_005633.3(SOS1): c.1649T > 6654 SOS1 [′ATATCTTTACAGTACCGGAGTACACYGGAA C (p.Leu550Pro) AGGATGCTTGATGTAACAATG′] 1937 NM_005633.3(SOS1): c.2104T > 6654 SOS1 [′TCGGCACTGGGTAGAGCACCACTTCYATGAT C (p.Tyr702His) TTTGAAAGAGATGCATATCT′] 1938 NM_002755.3(MAP2K1): c.388T > 5604 MAP2K1 [′CAACTCTCCGTACATCGTGGGCTTCYATGGT C (p.Tyr130His) GCGTTCTACAGCGATGGCGA′] 1939 NC_012920.1: m.7505T > C 4574 MT-TS1 [′TTTCAAGCCAACCCCATGGCCTCCAYGACTT TTTCAAAAAGGTATTAGAAA′] 1940 NM_000084.4(CLCN5): c.674T > 1184 CLCN5 [′GTGGCTTGCTGCTGTGGGAACATCCYGTGCC C (p.Leu225Pro) ACTGCTTCAACAAATACAGG′] 1941 NM_000530.6(MPZ): c.89T > C 4359 MPZ [′GCAGTGCTGTCCCCGGCCCAGGCCAYCGTG (p.Ile30Thr) GTTTACACCGACAGGGAGGTC′] 1942 NM_000530.6(MPZ): c.244T > C 4359 MPZ [′CCCTCATTCCTCATAGATCTTCCACYATGCC (p.Tyr82His) AAGGGACAACCCTACATTGA′] 1943 NM_000530.6(MPZ): c.266T > C 4359 MPZ [′CACTATGCCAAGGGACAACCCTACAHTGAC (p.Ile89Thr) GAGGTGGGGACCTTCAAAGAG′] 1944 NM_000748.2(CHRNB2): c.923T > 1141 CHRNB2 [′TACCTCATGTTCACCATGGTGCTTGYCACCT C (p.Val308Ala) TCTCCATCGTCACCAGCGTG′] 1945 NM_003611.2(OFD1): c.111 + 2T > 8481 OFD1 [′CGGGGTATACTGGATACACTCAAGGYATCG C GATTTAGGCGTATCTGTGTCA′] 1946 NM_003611.2(OFD1): c.274T > C 8481 OFD1 MAGATGTGGCTATGAATATTCACTTYCTGTT (p.Ser92Pro) TTCTTTCCAGAAAGTGGTTT′] 1947 NM_024408.3(NOTCH2): c.1117T > 4853 NOTCH2 [′GCCCCACCCTGTGACAGGTCTCCTGYGTCAT C (p.Cys373Arg) CTGGATGATGCATGCATCAG′] 1948 NM_024408.3(NOTCH2): c.1438T > 4853 NOTCH2 [′TCTGGATAAGATTGGAGGCTTCACAYGTCTG C (p.Cys480Arg) TGCATGCCAGGTAAATGGGC′] 1949 NM_000540.2(RYR1): c.9242T > 6261 RYR1 [′CCCCGCTGCCCTTCTAGGACAGTGAYGAAGT C (p.Met3081Thr) CAGGCCCTGAGATCGTGAAG′] 1950 NM_000096.3(CP): c.650T > C 1356 CP [′AAAGAAAAACATATTGACCGAGAATYTGTG (p.Phe217Ser) GTGATGTTTTCTGTGGTGGAT′] 1951 NM_000096.3(CP): c.548T > C 1356 CP [′GTGACTAGGATTTACCATTCCCACABTGATG (p.Ile183Thr) CTCCAAAAGATATTGCCTCA′] 1952 NM_000540.2(RYR1): c.1205T > 6261 RYR1 [′CAGGAGGAGTCCCAGGCCGCCCGCAYGATC C (p.Met402Thr) CACAGCACCAATGGCCTATAC′] 1953 NM_006517.4(SLC16A2): c.1253T > 6567 SLC16A2 [′TTCGGGGGCCTTATCGTCGTCTGTCYTTTCCT C (p.Leu418Pro) GGGCCTTTGCGATGGCTTC′] 1954 NM_000096.3(CP): c.1123T > C 1356 CP [′CCGTGGGAAGCATGTTAGACACTACYACAT (p.Tyr375His) TGCCGCTGAGGAAATCATCTG′] 1955 NM_002739.3(PRKCG): c.391T > 5582 PRKCG [′CGGGCTTGTGCACCAGGGCATGAAAHGCTC C (p.Cys131Arg) CTGTGAGTGACCTGGGCCTTG′] 1956 NM_000116.4(TAZ): c.310T > C 6901 TAZ [′GACCCCTGCAGCTGCAGACATCTGCYTCACC (p.Phe104Leu) AAGGAGCTACACTCCCACTT′] 1957 NM_000138.4(FBN1): c.2341T > 2200 FBN1 [′CAGTCTCCTTTGTGACAATGGACAAYGTAGA C (p.Cys781Arg) AATACTCCTGGAAGTTTTGT′] 1958 NM_000138.4(FBN1): c.4222T > 2200 FBN1 [′CTTTCTGGCTGTAGACCTTGATGAGYGCTCT C (p.Cys1408Arg) GAGAACCTGAATCTCTGTGG′] 1959 NM_000256.3(MYBPC3): c.1351 + 4607 MYBPC3 [′GTAGCACGGAGCTCTTTGTGAAAGGYGGGC 2T > C CTGGGACCTGAGGATGTGGGA′] 1960 NM_000256.3(MYBPC3): c.3392T > 4607 MYBPC3 [′GACGCGGAAGTAGTAGCCATTGCCARTGAT C (p.Ile1131Thr) GAGCTCTGGCACCACGCAGTG′] 1961 NM_000256.3(MYBPC3): c.821 + 4607 MYBPC3 [′CCTCCTATCAGCCTTCCGCCGCACGYGAGTG 2T > C GCCATCCTCAGGGCCTGGGG′] 1962 NM_000257.3(MYH7): c.1370T > 4625 MYH7 [′ACCAAGCAGCCACGCCAGTACTTCAYAGGA C (p.Ile457Thr) GTCCTGGACATCGCTGGCTTC′] 1963 NM_000257.3(MYH7): c.2093T > 4625 MYH7 [′ATGCACCAGCTGCGCTGCAATGGTGYGCTG C (p.Val698Ala) GAGGGCATCCGCATCTGCAGG′] 1964 NM_000257.3(MYH7): c.2546T > 4625 MYH7 [′AAGAGTGCAGAAAGAGAGAAGGAGAYGGC C (p.Met849Thr) CTCCATGAAGGAGGAGTTCACA′] 1965 NM_000257.3(MYH7): c.2555T > 4625 MYH7 [′GAAAGAGAGAAGGAGATGGCCTCCAHGAA C (p.Met852Thr) GGAGGAGTTCACACGCCTCAAA′] 1966 NM_000257.3(MYH7): c.602T > 4625 MYH7 [′CAGTACTTTGCTGTTATTGCAGCCAYTGGGG C (p.Ile201Thr) ACCGCAGCAAGAAGGACCAG′] 1967 NM_000257.3(MYH7): c.788T > 4625 MYH7 [′ACAGGAAAGTTGGCATCTGCAGACAYAGAG C (p.Ile263Thr) ACCTGTGAGTGCCATGAATCT′] 1968 NM_000260.3(MY07A): c.5573T > 4647 MY07A [′CTCCTGCCCCACGTGCAGCGCTTCCYGCAGT C (p.Leu1858Pro) CCCGAAAGCACTGCCCACTC′] 1969 NM_000441.1(SLC26A4): c.164 + 5172 SLC26A4 [′GGAGAGCCTGGCCAAGTGCTGCAGGYAGCG 2T > C GCCGCGCGGGCCTGCGTAGAG′] 1970 NM_000441.1(SLC26A4): c.2T > −1 — [′TCGCTGTCCTCTGGCTCGCAGGTCABGGCAG C (p.Met1Thr) CGCCAGGCGGCAGGTCGGAG′] 1971 NM_000441.1(SLC26A4): c.765 + 5172 SLC26A4 [′AATGGAGTTCTCTCTATTATCTATGYAAGTG 2T > C TTGCTTCTTGCTCCAGGGAT′] 1972 NM_000551.3(VHL): c.497T > C 7428 VHL [′CTGAAAGAGCGATGCCTCCAGGTTGYCCGG (p.Val166Ala) AGCCTAGTCAAGCCTGAGAAT′] 1973 NM_001399.4(EDA): c.2T > C 1896 EDA [′AGAGAGTGGGTGTCTCCGGAGGCCAYGGGC (p.Met1Thr) TACCCGGAGGTGGAGCGCAGG′] 1974 NM_001943.3(DSG2): c.523 + 2T > 1829 DSG2 [′CTGTTGAAGAGTTGAGTGCAGCACGYAAGA C GTCTTTTTTTTTTTTTTTAAT′] 1975 NM_004572.3(PKP2): c.2062T > 5318 PKP2 [′TTGCGGACACTTTTGGCGATCAAGGRCAGAT C (p.Ser688Pro) ACATCCTTATAACAATGGAA′] 1976 NM_005633.3(SOS1): c.1310T > 6654 SOS1 [′ATTGATGGTTGGGAGGGAAAAGACABTGGA C (p.Ile437Thr) CAGTGTTGTAATGAATTTATA′] 1977 NM_170707.3(LMNA): c.799T > 4000 LMNA [GTATAAGAAGGAGCTGGAGAAGACTYATTC C (p.Tyr267His) TGCCAAGGTGCTTGCTCTCGA′] 1978 NM_206933.2(USH2A): c.10561T > 7399 USH2A [′TCTTGAAGATACAATTGTCTTAAACYGGAGA C (p.Trp3521Arg) AAACCTATACAATCAAATGG′] 1979 NM_206933.2(USH2A): c.1606T > 7399 USH2A [′CGACACAACAAGCCAGCCATATAGAYGCCT C (p.Cys536Arg) CTGCTCCCAGGAGAGCTTCAC′] 1980 NM_206933.2(USH2A): c.5857 + −1 — [′GTCGAGGACGTACAACAGGAGCAGGYAAAT 2T > C ACTTATCTTCAAATGCATATG′] 1981 NM_000492.3(CFTR): c.1090T > 1080 CFTR [′CTGGGCTGTACAAACATGGTATGACYCTCTT C (p.Ser364Pro) GGAGCAATAAACAAAATACA′] 1982 NM_000548.3(TSC2): c.5150T > 7249 TSC2 [′CCCTTCGTGGCCCGCCAGATGGCCCYGCACG C (p.Leu1717Pro) CAAATGTGAGTGGGGGTGGG′] 1983 NM_000548.3 (TSC2): c.2410T > 7249 TSC2 [′CCTCATCCACCGCTGTGCCAGCCAGYGCGTC C (p.Cys804Arg) GTGGCCTTGTCCATCTGCAG′] 1984 NM_000548.3(TSC2): c.3106T > 7249 TSC2 [′GGACATGATGGCTCGATACGTCTTCYCCAAC C (p.Ser1036Pro) TTCACGGCTGTCCCGAAGAG′] 1985 NM_000212.2(ITGB3): c.2231T > −1 — [′GCCGCCCTGCTCATCTGGAAACTCCYCATCA C (p.Leu744Pro) CCATCCACGACCGAAAAGAA′] 1986 NM_183415.2(UBE3B): c.1741 + 89910 UBE3B [′AGATGATCTGGGATGGAATTGTAGGYAAGA 2T > C GAAAAGGTGTCTGCTGTTGTT] 1987 NM_005120.2(MED12): c.3493T > 9968 MED12 [′CCTTTTAGCTTGTAGTGAACAGGACYCTGAG C (p.Ser1165Pro) CCAGGGGCCCGGCTTACCTG′] 1988 NM_001033053.2(NLRP1): c.230T > 22861 NLRP1 [′CTAGCCCTCCATACCTGGGAGCAGAYGGGG C (p.Met77Thr) CTGAGGTCACTGTGCGCCCAA′] 1989 NM_025152.2(NUBPL): c.815 − 80224 NUBPL [′ATGCCTATATGAACTTTTCTGGTTCYAATGG 27T > C ATGTCTGCTGGGCTCTTTTA′] 1990 NM_001288953.1(TTC7A): c.2366T > 57217 TTC7A [′GCGTGGCAGGGCCTGGGCGAGGTGCYGCAG C (p.Leu789Pro) GCCCAGGGCCAGAACGAGGCT′] 1991 NM_014845.5(FIG4): c.524T > C 9896 FIG4 [′TACAGCTATGATTTGTCCCACTCACYTCAAT (p.Leu175Pro) ATAATCTCACTGTCTTGCGA′] 1992 NM_000059.3(BRCA2): c.2T > C 675 BRCA2 [′ATTGGAGGAATATCGTAGGTAAAAABGCCT (p.Met1Thr) ATTGGATCCAAAGAGAGGCCA′] 1993 NM_000059.3(BRCA2): c.316 + 675 BRCA2 [′TAGATAAATTCAAATTAGACTTAGGYAAGT 2T > C AATGCAATATGGTAGACTGGG′] 1994 NM_000059.3(BRCA2): c.67 + 2T > 675 BRCA2 [′TTAAGACACGCTGCAACAAAGCAGGHATTG C ACAAATTTTATATAACTTTAT′] 1995 NM_000059.3(BRCA2): c.7958T > 675 BRCA2 [′AGATGCCTAAGCCCAGAAAGGGTGCYTCTT C (p.Leu2653Pro) CAACTAAAATACAGGCAAGTT′] 1996 NM_000218.2(KCNQ1): c.1016T > 3784 KCNQ1 [′TGCTTCTCTGTCTTTGCCATCTCCTHCTTTGC C (p.Phe339Ser) GCTCCCAGCGGTAGGTGCC′] NM_000218.2(KCNQ1): c.1045T > 3784 KCNQ1 [] C (p.Ser349Pro) NM_000218.2(KCNQ1): c.1052T > 3784 KCNQ1 [] C (p.Phe351Ser) NM_000218.2(KCNQ1): c.1058T > 3784 KCNQ1 [] C (p.Leu353Pro) NM_000218.2(KCNQ1): c.1541T > 3784 KCNQ1 [] C (p.Ile514Thr) NM_000218.2(KCNQ1): c.1661T > 3784 KCNQ1 [] C (p.Val554Ala) 1997 NM_000218.2(KCNQ1): c.1700T > 3784 KCNQ1 [′CTTCTCTCCAGGCTGGACCAGTCCABTGGGA C (p.Ile567Thr) AGCCCTCACTGTTCATCTCC′] NM_000218.2(KCNQ1): c.2T > C 3784 KCNQ1 [] (p.Met1Thr) NM_000218.2(KCNQ1): c.341T > 3784 KCNQ1 [] C (p.Leu114Pro) 1998 NM_000218.2(KCNQ1): c.560T > 3784 KCNQ1 [′GGCTGCCGCAGCAAGTACGTGGGCCYCTGG C (p.Leu187Pro) GGGCGGCTGCGCTTTGCCCGG′] NM_000218.2(KCNQ1): c.572T > 3784 KCNQ1 [] C (p.Leu191Pro) NM_000218.2(KCNQ1): c.716T > 3784 KCNQ1 [] C (p.Leu239Pro) NM_000218.2(KCNQ1): c.752T > 3784 KCNQ1 [] C (p.Leu251Pro) NM_000218.2(KCNQ1): c.797T > 3784 KCNQ1 [] C (p.Leu266Pro) NM_000218.2(KCNQ1): c.824T > 3784 KCNQ1 [] C (p.Phe275Ser) NM_000218.2(KCNQ1): c.910T > 3784 KCNQ1 [] C (p.Trp304Arg) NM_000218.2(KCNQ1): c.913T > 3784 KCNQ1 [] C (p.Trp305Arg) 1999 NM_000492.3(CFTR): c.1021T > 1080 CFTR [′CCTCCGGAAAATATTCACCACCATCYCATTC C (p.Ser341Pro) TGCATTGTTCTGCGCATGGC′] 2000 NM_000492.3(CFTR): c.1853T > 1080 CFTR [′TTAAAGAAAGCTGACAAAATATTAAYTTTG C (p.Ile618Thr) CATGAAGGTAGCAGCTATTTT′] 2001 NM_000492.3(CFTR): c.2780T > 1080 CFTR [′TACGTGGGAGTAGCCGACACTTTGCYTGCTA C (p.Leu927Pro) TGGGATTCTTCAGAGGTCTA′] 2002 NM_000492.3(CFTR): c.3230T > 1080 CFTR [′GGACGGCAGCCTTACTTTGAAACTCYGTTCC C (p.Leu1077Pro) ACAAAGCTCTGAATTTACAT′] 2003 NM_007294.3(BRCA1): c.115T > 672 BRCA1 [′GATCAAGGAACCTGTCTCCACAAAGNGTGA C (p.Cys39Arg) CCACATATTTTGCAAGTAAGT′] 2004 NM_007294.3(BRCA1): c.190T > 672 BRCA1 [′GAAAGGGCCTTCACAGTGTCCTTTABGTAAG C (p.Cys64Arg) AATGATATAACCAAAAGGTA′] 2005 NM_007294.3(BRCA1): c.212 + 672 BRCA1 [′ATGTAAGAATGATATAACCAAAAGGYATAT 2T > C AATTTGGTAATGATGCTAGGT′] 2006 NM_007294.3(BRCA1): c.2T > C 672 BRCA1 [′TAAAGTTCATTGGAACAGAAAGAAABGGAT (p.Met1Thr) TTATCTGCTCTTCGCGTTGAA′] 2007 NM_007294.3(BRCA1): c.4357 + 672 BRCA1 [′CAGAACAAAGCACATCAGAAAAAGGBGTGT 2T > C ATTGTTGGCCAAACACTGATA′] 2008 NM_007294.3 (BRCA1): c.5291T > 672 BRCA1 [′CTCTTCTTCCAGATCTTCAGGGGGCYAGAAA C (p.Leu1764Pro) TCTGTTGCTATGGGCCCTTC′] 2009 NM_007294.3(BRCA1): c.65T > C 672 BRCA1 [′GTCATTAATGCTATGCAGAAAATCTYAGAGT (p.Leu22Ser) GTCCCATCTGGTAAGTCAGC′] 2010 NM_005236.2(ERCC4): c.689T > 2072 ERCC4 [′ATGCTTGCTATACAGACTGCTATACYGGACA C (p.Leu230Pro) TTTTAAATGCATGTCTAAAG′] 2011 NM_005236.2(ERCC4): c.706T > 2072 ERCC4 [′TGCTATACTGGACATTTTAAATGCAYGTCTA C (p.Cys236Arg) AAGGAACTAAAATGCCATAA′] 2012 NM_000435.2(NOTCH3): c.4556T > 4854 NOTCH3 [′GGCGTGCTGGTGCTCACAGTGCTGCYGCCGC C (p.Leu1519Pro) CAGAGGAGCTACTGCGTTCC′] 2013 NM_153704.5(TMEM67): c.1046T > 91147 TMEM67 [′GGAAATTTTCTCAAGTGGCAAACTTYAGAA C (p.Leu349Ser) GGAGGTGTTTTACAGGTAAGC′] 2014 NM_001142519.1(FAM111A): c. 63901 FAM111A [′GTCTAAAAAAGCAGAAAGTCCAGAGYATGT 1531T > C (p.Tyr511His) CCATATGTATACTCAAAGAAG′] 2015 NM_014795.3(ZEB2): c.3211T > 9839 ZEB2 [′GCGCTTCTCACACTCGGGCTCGTACYCGCAG C (p.Ser1071Pro) CACATGAATCACAGGTATTC′] 2016 NM_001199107.1(TBC1D24): c. 57465 TBC1D24 [′CCCCTCTGCTACTTCGCCCGGGTCTYTGACG 686T > C (p.Phe229Ser) TCTTCCTGGTGGAGGGCTAC′] 2017 NM_022114.3 (PRDM16): c.2660T > 63976 PRDM16 [′GTGGGAGCCCTGAAGGAGAAGTACCYGCGG C (p.Leu887Pro) CCGTCCCCGCTGCTCTTCCAC′] 2018 NM_001130089.1(KARS): c.517T > 3735 KARS [′AGCTTCTGGGGGAAAGCTCATCTTCYATGAT C (p.Tyr173His) CTTCGAGGAGAGGGGGTGAA′] 2019 NM_005689.2(ABCB6): c.1067T > 10058 ABCB6 [′CTCATCTTCTCCCACCTGCACGAGCYCTCAC C (p.Leu356Pro) TGCGCTGGCACCTGGGGCGC′] 2020 NM_001070.4(TUBG1): c.1160T > 7283 TUBG1 [′CCCCTGTTTTCTGCACACCCCAAGCYCTTCG C (p.Leu387Pro) AGAGAACCTGTCGCCAGTAT′] 2021 NM_001283009.1(RTEL1): c.3730T > −1 — [′CGGGCCCCTCTCAGCAGGCTGTGTGYGCCA C (p.Cys1244Arg) GGGCTGTGGGGCAGAGGACGT′] 2022 NM_001135021.1(ELMOD3): c. 84173 ELMOD3 [′ATCACCCACATTGCCATCCAGGCCTYGAGA 794T > C (p.Leu265Ser) GAGGAGTGTCTCTCCAGGTGA′] 2023 NM_001382.3 (DPAGT1): c.503T > 1798 DPAGT1 [′CTACCATCTCTCCCCGCAGGAATCCYGTACT C (p.Leu168Pro) ATGTCTACATGGGGCTGCTG′] 2024 m.10237T > C 4537 MT-ND3 [′CCATAAAATTCTTCTTAGTAGCTATYACCTT CTTATTATTTGATCTAGAAA′] 2025 m.11253T > C 4538 MT-ND4 [′TCCCTTCCCCTACTCATCGCACTAAYTTACA CTCACAACACCCTAGGCTCA′] 2026 m.12811T > C 4540 MT-ND5 [′ATCCTTCTTGCTCATCAGTTGATGAYACGCC CGAGCAGATGCCAACACAGC′] 2027 m.14325T > C 4541 MT-ND6 [′ATTCAGCTTCCTACACTATTAAAGTYTACCA CAACCACCACCCCATCATAC′] 2028 NM_000142.4(FGFR3): c.2419T > 2261 FGFR3 [′ACCCAGCAGTGGGGGCTCGCGGACGNGAAG C (p.Ter807Arg) GGCCACTGGTCCCCAACAATG′] 2029 NM_000370.3 (TTPA): c.548T > C 7274 TTPA [′GTAGCCAAGAAGATTGCTGCTGTACYTACG (p.Leu183Pro) GTAAATGTATATTTTAACTGT′] 2030 NM_000375.2(UROS): c.139T > C 7390 UROS [′TGAGTTTTTGTCTCTTCCCAGTTTCYCTGAGA (p.Ser47Pro) AGGTAAGGCCTGTTGTGAC′] 2031 NM_001006657.1(WDR35): c. 57539 WDR35 [′TCTGGCACCATTCAGAGATACAGTCYACCTA 1592T > C (p.Leu531Pro) ATGTTGGTTTGATTCAAAAA′] 2032 NM_001876.3 (CPT1A): c.1451T > 1374 CPT1A [′GCAGATGCGCCGATCGTGGCCCACCYTTGG C (p.Leu484Pro) GAGGTGAGTTTTCACACTTTT′] 2033 NM_004595.4(SMS): c.449T > C 6611 SMS [′GACGAAGATTCACCTTATCAAAATAYAAAA (p.Ile150Thr) ATTCTACACTCGAAGCAGTTT′] 2034 NM_005211.3 (C SF1R): c.2483T > 1436 CSF1R [′AAGTGGATGGCCCCAGAGAGCATCTYTGAC C (p.Phe828Ser) TGTGTCTACACGGTTCAGAGC′] 2035 NM_170707.3 (LMNA): c.644T > 4000 LMNA [′TCCTTCCTCCAACCCTTCCAGGAGCYGCGTG C (p.Leu215Pro) AGACCAAGCGCCGTCATGAG′] 2036 NM_194248.2(0T0F): c.3413T > 9381 OTOF [′CCACCCTCCAACCTCTCCCAGGTGCYGTTCT C (p.Leu1138Pro) GGGGCCTACGGGACCTAAAG′] 2037 NM_000540.2(RYR1): c.14762T > 6261 RYR1 [TACAGGGTGGTCTTCGACATCACCTYCTTCT C (p.Phe4921Ser) TCTTCGTCATCGTCATCCTG′] 2038 NM_000540.2(RYR1): c.7358T > 6261 RYR1 [′GCCGGCAAGGGTGAGGCCCTGCGGAYCCGC C (p.Ile2453Thr) GCCATCCTCCGCTCCCTTGTG′] 2039 NM_000540.2(RYR1): c.10817T > [′GAAGTGTCAGCCGTGCTCTACTACCYGGACC C (p.Leu3606Pro) 6261 RYR1 AGGTGGGTGGGGCCGGAGGG′] 2040 NM_000540.2(RYR1): c.13703T > [′TTTTACACCCTGCGGTTCCTTGCCCYCTTCTT C (p.Leu4568Pro) 6261 RYR1 GGCATTTGCCATCAACTTC′] 2041 NM_000540.2(RYR1): c.13949T > [′CCCGCCCTGCGGTGTCTGAGCCTCCYGCATA C (p.Leu4650Pro) 6261 RYR1 CACTGGTGGCCTTTCTCTGC′] 2042 NM_000540.2(RYR1): c.14378T > [′GTGTGCCCACAGTCCTTCCTGTACCYGGGCT C (p.Leu4793Pro) 6261 RYR1 GGTATATGGTGATGTCCCTC′] 2043 NM_015896.3(ZMYND10): c.797T > [′CTCAGGGCTTAGCAGCAGGTTGTACRGGGC C (p.Leu266Pro) 51364 ZMYND10 GATCCACACTTGCCCGTCCAA′] 2044 NM_018127.6(ELAC2): c.460T > [′AAAATACCTCGAAGCAATCAAAATAYTTTCT C (p.Phe154Leu) 60528 ELAC2 GGTCCATTGAAAGGAATAGA′] 2045 NM_199355.2(ADAMTS18): c.605T > [′CCTGCGGGTCACCATCCTCACGTACYGTACA C (p.Leu202Pro) 170692 ADAMTS18 AAAGGACAGCAGAGGAGAAG] 2046 NM_023110.2(FGFR1): c.494T > [′TCCCCAGAAAAGATGGAAAAGAAATYGCAT C (p.Leu165Ser) 2260 FGFR1 GCAGTGCCGGCTGCCAAGACA′] 2047 NM_001059.2(TACR3): c.766T > [′CCATATTATCGTCATTATACTGGTGYACTGT C (p.Tyr256His) 6870 TACR3 TTCCCATTGCTCATCATGGG′] 2048 NM_002055.4(GFAP): c.1070T > [′TACCAGGACCTGCTCAATGTCAAGCHGGCC C (p.Leu357Pro) 2670 GFAP CTGGACATCGAGATCGCCACC′] 2049 NM_002055.4(GFAP): c.1076T > [′GACCTGCTCAATGTCAAGCTGGCCCYGGAC C (p.Leu359Pro) 2670 GFAP ATCGAGATCGCCACCTACAGG′] 2050 NM_002055.4(GFAP): c.1096T > [′GGCCCTGGACATCGAGATCGCCACCYACAG C (p.Tyr366His) 2670 GFAP GAAGCTGCTAGAGGGCGAGGA′] 2051 NM_002055.4(GFAP): c.221T > C [′CGGGCCAGTGAGCGGGCAGAGATGAYGGAG (p.Met74Thr) 2670 GFAP CTCAATGACCGCTTTGCCAGC] 2052 NM_002055.4(GFAP): c.247T > C [′GGAGCTCAATGACCGCTTTGCCAGCYACATC (p.Tyr83His) 2670 GFAP GAGAAGGTTCGCTTCCTGGA′] 2053 NM_002055.4(GFAP): c.269T > C [′AGCTACATCGAGAAGGTTCGCTTCCYGGAA (p.Leu90Pro) 2670 GFAP CAGCAAAACAAGGCGCTGGCT′] 2054 NM_002055.4(GFAP): c.290T > C [′TTCCTGGAACAGCAAAACAAGGCGCYGGCT (p.Leu97Pro) 2670 GFAP GCTGAGCTGAACCAGCTGCGG′] 2055 NM_002055.4(GFAP): c.302T > C [′CAAAACAAGGCGCTGGCTGCTGAGCYGAAC (p.Leu101Pro) 2670 GFAP CAGCTGCGGGCCAAGGAGCCC′] 2056 NM_002055.4(GFAP): c.704T > C [′GCCAAGCCAGACCTCACCGCAGCCCYGAAA (p.Leu235Pro) 2670 GFAP GAGATCCGCACGCAGTATGAG′] 2057 NM_002055.4(GFAP): c.739T > C [′CCGCACGCAGTATGAGGCAATGGCGYCCAG (p.Ser247Pro) 2670 GFAP CAACATGCATGAAGCCGAAGA′] 2058 NM_002055.4(GFAP): c.992T > C [′AGTTATCAGGAGGCGCTGGCGCGGCYGGAG (p.Leu331Pro) 2670 GFAP GAAGAGGGGCAGAGCCTCAAG′] 2059 NM_005554.3(KRT6A): c.1406T > [′GAGATCGCCACCTACCGCAAGCTGCBGGAG C (p.Leu469Pro) 3853 KRT6A GGTGAGGAGTGCAGGTGGGTA′] 2060 NM_005554.3(KRT6A): c.521T > 3853 KRT6A [′CAGATCAAGACCCTCAACAACAAGTBTGCC C (p.Phe174Ser) TCCTTCATCGACAAGGTGAGC′] 2061 NM_153490.2(KRT13): c.332T > 3860 KRT13 [′AATGAGAAGATCACCATGCAGAACCYCAAC C (p.Leu111Pro) GACCGCCTGGCTTCCTACCTG′] 2062 NM_170707.3(LMNA): c.1619T > 4000 LMNA [′CCATGTCCCCACCAGGAAGTGGCCAYGCGC C (p.Met540Thr) AAGCTGGTGCGCTCAGTGACT′] NM_000218.2(KCNQ1): c.1117T > 3784 KCNQ1 [] C (p.Ser373Pro) 2063 NM_000218.2(KCNQ1): c.1135T > 3784 KCNQ1 [′TCCCCCTGCCCGACCTCAGACCGCABGGAG C (p.Trp379Arg) GTGCTATGCTGCCGAGAACCC′] NM_000218.2(KCNQ1): c.1165T > 3784 KCNQ1 [] C (p.Ser389Pro) NM_000218.2(KCNQ1): c.1174T > 3784 KCNQ1 [] C (p.Trp392Arg) NM_000218.2(KCNQ1): c.1550T > 3784 KCNQ1 [] C (p.Ile517Thr) NM_000218.2(KCNQ1): c.1696T > 3784 KCNQ1 [] C (p.Ser566Pro) NM_000218.2(KCNQ1): c.1805T > −1 — [] C (p.Leu602Pro) NM_000218.2(KCNQ1): c.401T > 3784 KCNQ1 [] C (p.Leu134Pro) 2064 NM_000218.2(KCNQ1): c.550T > 3784 KCNQ1 [′CTGGTCCGCCGGCTGCCGCAGCAAGBACGT C (p.Tyr184His) GGGCCTCTGGGGGCGGCTGCG′] NM_000218.2(KCNQ1): c.608T > 3784 KCNQ1 [] C (p.Leu203Pro) NM_000218.2(KCNQ1): c.625T > 3784 KCNQ1 [] C (p.Ser209Pro) NM_000218.2(KCNQ1): c.742T > 3784 KCNQ1 [] C (p.Trp248Arg) 2065 NM_000218.2(KCNQ1): c.749T > 3784 KCNQ1 [′GACCGCCAGGGAGGCACCTGGAGGCHCCTG C (p.Leu250Pro) GGCTCCGTGGTCTTCATCCAC′] NM_000218.2(KCNQ1): c.829T > 3784 KCNQ1 [] C (p.Ser277Pro) NM_000218.2(KCNQ1): c.832T > 3784 KCNQ1 [] C (p.Tyr278His) NM_000218.2(KCNQ1): c.845T > 3784 KCNQ1 [] C (p.Leu282Pro) NM_000218.2(KCNQ1): c.908T > 3784 KCNQ1 [] C (p.Leu303Pro) 2066 NM_000238.3(KCNH2): c.122T > 3757 KCNH2 [′AACGCTCGGGTGGAGAACTGCGCCGBCATC C (p.Val41Ala) TACTGCAACGACGGCTTCTGC′] NM_000238.3(KCNH2): c.1238T > 3757 KCNH2 [] C (p.Leu413Pro) NM_000238.3(KCNH2): c.1279T > 3757 KCNH2 [] C (p.Tyr427His) NM_000238.3 (KCNH2): c.1387T > 3757 KCNH2 [] C (p.Phe463Leu) NM_000238.3 (KCNH2): c.160T > 3757 KCNH2 [] C (p.Tyr54His) 2067 NM_000238.3 (KCNH2): c.1655T > 3757 KCNH2 [′GAGTACGGCGCGGCCGTGCTGTTCTYGCTCA C (p.Leu552Ser) TGTGCACCTTTGCGCTCATC′] NM_000238.3 (KCNH2): c.1691T > 3757 KCNH2 [] C (p.Leu564Pro) NM_000238.3 (KCNH2): c.1700T > 3757 KCNH2 [] C (p.Ile567Thr) NM_000238.3 (KCNH2): c.1702T > 3757 KCNH2 [] C (p.Trp568Arg) NM_000238.3 (KCNH2): c.1705T > 3757 KCNH2 [] C (p.Tyr569His) NM_000238.3 (KCNH2): c.1736T > 3757 KCNH2 [] C (p.Met579Thr) 2068 NM_000238.3 (KCNH2): c.1778T > 3757 KCNH2 [′TGGCTGCACAACCTGGGCGACCAGANAGGC C (p.Ile593Thr) AAACCCTACAACAGCAGCGGC] NM_000238.3 (KCNH2): c.1816T > 3757 KCNH2 [] C (p.Ser606Pro) 2069 NM_000238.3 (KCNH2): c.1879T > 3757 KCNH2 [′CTTCAGCAGCCTCACCAGTGTGGGCHTCGGC C (p.Phe627Leu) AACGTCTCTCCCAACACCAM NM_000238.3 (KCNH2): c.1889T > 3757 KCNH2 [] C (p.Val630Ala) NM_000238.3 (KCNH2): c.1945T > 3757 KCNH2 [] C (p.Ser649Pro) NM_000238.3 (KCNH2): c.1985T > 3757 KCNH2 [] C (p.Ile662Thr) NM_000238.3 (KCNH2): c.202T > 3757 KCNH2 [] C (p.Phe68Leu) NM_000238.3(KCNH2): c.2033T > 3757 KCNH2 [] C (p.Leu678Pro) NM_000238.3 (KCNH2): c.206T > 3757 KCNH2 [] C (p.Leu69Pro) NM_000238.3(KCNH2): c.2078T > 3757 KCNH2 [] C (p.Leu693Pro) NM_000238.3(KCNH2): c.2309T > 3757 KCNH2 [] C (p.Val770Ala) 2070 NM_000238.3 (KCNH2): c.2414T > 3757 KCNH2 [′TGGCCTCCAGGGAAGAATGACATCTBTGGG C (p.Phe805Ser) GAGCCTCTGAACCTGTATGCA′] NM_000238.3(KCNH2): c.2452T > 3757 KCNH2 [] C (p.Ser818Pro) NM_000238.3(KCNH2): c.2573T > 3757 KCNH2 [] C (p.Ile858Thr) 2071 NM_000238.3(KCNH2): c.257T > 3757 KCNH2 [′GCTGCCGCGCAGATCGCGCAGGCACBGCTG C (p.Leu86Pro) GGCGCCGAGGAGCGCAAAGTG′] NM_000238.3(KCNH2): c.260T > 3757 KCNH2 [] C (p.Leu87Pro) NM_000238.3(KCNH2): c.287T > 3757 KCNH2 [] C (p.Ile96Thr) NM_000238.3(KCNH2): c.3146T> 3757 KCNH2 [] C (p.Leu1049Pro) NM_000238.3(KCNH2): c.322T > 3757 KCNH2 [] C (p.Cys108Arg) 2072 NM_000238.3(KCNH2): c.371T > 3757 KCNH2 [′AAGAACGAGGATGGGGCTGTCATCABGTTC C (p.Met124Thr) ATCCTCAATTTCGAGGTGGTG′] NM_000238.3(KCNH2): c.65T > 3757 KCNH2 [] C (p.Phe22Ser) NM_000238.3(KCNH2): c.86T > 3757 KCNH2 [] C (p.Phe29Ser) NM_000238.3 (KCNH2): c.872T > 3757 KCNH2 [] C (p.Met291Thr) NM_000238.3(KCNH2): c.89T > 3757 KCNH2 [] C (p.Ile30Thr) 2073 NM_000238.3 (KCNH2): c.92T > 3757 KCNH2 [′GCCCCCCTAGGCCGTAAGTTCATCABCGCCA C (p.Ile31Thr) ACGCTCGGGTGGAGAACTGC′] NM_000891.2(KCNJ2): c.301T > 3759 KCNJ2 [] C (p.Cys101Arg) NM_000891.2(KCNJ2): c.650T > 3759 KCNJ2 [] C (p.Leu217Pro) NM_000335.4(SCN5A): c.1187T > 6331 SCN5A [] C (p.Val396Ala) NM_000335.4(SCN5A): c.1190T > 6331 SCN5A [] C (p.Ile397Thr) NM_000335.4(SCN5A): c.2018T > 6331 SCN5A [] C (p.Leu673Pro) NM_000335.4(SCN5A): c.2516T > 6331 SCN5A [] C (p.Leu839Pro) NM_000335.4(SCN5A): c.2551T > 6331 SCN5A [] C (p.Phe851Leu) NM_000335.4(SCN5A): c.2743T > 6331 SCN5A [] C (p.Cys915Arg) NM_000335.4(SCN5A): c.2783T > 6331 SCN5A [] C (p.Leu928Pro) NM_000335.4(SCN5A): c.278T > 6331 SCN5A [] C (p.Phe93Ser) NM_000335.4(SCN5A): c.2804T > 6331 SCN5A [] C (p.Leu935Pro) 2074 NM_000335.4(SCN5A): c.2944T > 6331 SCN5A [′CAAGCGGACCACCTGGGATTTCTGCYGTGGT C (p.Cys982Arg) CTCCTGCGGCAGCGGCCTCA′] 2075 NM_198056.2(SCN5A): c.3010T > 6331 SCN5A [′TGCCGCCCAGGGCCAGCTGCCCAGCYGCAT C (p.Cys1004Arg) TGCCACCCCCTACTCCCCGCC′] 2076 NM_000335.4(SCN5A): c.3679T > 6331 SCN5A [′TTGTCTGCAGGCCTTCGAGGACATCYACCTA C (p.Tyr1227His) GAGGAGCGGAAGACCATCAA′] NM_000335.4(SCN5A): c.3713T > C (p.Leu1238Pro) 6331 SCN5A [] 2077 NM_000335.4(SCN5A): c.3745T > 6331 SCN5A [′TGCCGACAAGATGTTCACATATGTCYTCGTG C (p.Phe1249Leu) CTGGAGATGCTGCTCAAGTG′] NM_000335.4(SCN5A): c.3929T > 6331 SCN5A [] C (p.Leu1310Pro) NM_000335.4(SCN5A): c.4027T > 6331 SCN5A [] C (p.Phe1343Leu) NM_000335.4(SCN5A): c.4028T > 6331 SCN5A [] C (p.Phe1343Ser) NM_000335.4(SCN5A): c.4034T > 6331 SCN5A [] C (p.Leu1345Pro) NM_000335.4(SCN5A): c.4046T > 6331 SCN5A [] C (p.Ile1349T1ir) NM_000335.4(SCN5A): c.407T > 6331 SCN5A [] C (p.Leu136Pro) NM_000335.4(SCN5A): c.4340T > 6331 SCN5A [] C (p.Ile1447T1ir) 2078 NM_000335.4(SCN5A): c.4415T > 6331 SCN5A [′TTTATTGGTGTCATCATTGACAACTBCAACC C (p.Phe1472Ser) AACAGAAGAAAAAGATACGT′] NM_000335.4(SCN5A): c.4453T > 6331 SCN5A [] C (p.Phe1485Leu) NM_198056.2(SCN5A): c.4493T > 6331 SCN5A [] C (p.Met1498T1ir) NM_000335.4(SCN5A): c.4742T > 6331 SCN5A [] C (p.Leu1581Pro) NM_000335.4(SCN5A): c.4778T > 6331 SCN5A [] C (p.Phe1593Ser) 2079 NM_000335.4(SCN5A): c.4952T > 6331 SCN5A [′CGCACGCTGCTCTTTGCCCTCATGABGTCCC C (p.Met1651Thr) TGCCTGCCCTCTTCAACATC′] NM_000335.4(SCN5A): c.5111T > 6331 SCN5A [] C (p.Phe1704Ser) NM_000335.4(SCN5A): c.5179T > 6331 SCN5A [] C (p.Cys1727Arg) NM_000335.4(SCN5A): c.544T > 6331 SCN5A [] C (p.Cys182Arg) 2080 NM_000335.4(SCN5A): c.5471T > 6331 SCN5A [′TTTGCCGATGCCCTGTCTGAGCCACYCCGTA C (p.Leu1824Pro) TCGCCAAGCCCAACCAGATA′] 2081 NM_198056.2(SCN5A): c.5624T > 6331 SCN5A [′GAGATGGACGCCCTGAAGATCCAGAHGGAG C (p.Met1875Thr) GAGAAGTTCATGGCAGCCAAC′] 2082 NM_000335.4(SCN5A): c.635T > 6331 SCN5A [′AGATACACAACTGAATTTGTGGACCHGGGC C (p.Leu212Pro) AATGTCTCAGCCTTACGCACC′] NM_000335.4(SCN5A): c.689T > 6331 SCN5A [] C (p.Ile230Thr) NM_198056.2(SCN5A): c.944T > 6331 SCN5A [] C (p.Leu315Pro) 2083 NM_177976.2(ARL6): c.272T > C 84100 ARL6 [′TTACACAGAGAAGGCCAAGCTATTAYTTTTG (p.Ile91Thr) TCATTGATAGTAGTGATAGA′] 2084 NM_000487.5(ARSA): c.899T > C 410 ARSA [′TCCCGAGGCGGCTGCTCCGGTCTCTYGCGGT (p.Leu300Ser) GTGGAAAGGGAACGACCTAC′] 2085 NM_000267.3(NF1): c.1595T > C 4763 NF1 [′GAATTAATTACAGGGCTCGTCCAACYGGTCC (p.Leu532Pro) CTCAGTCACACATGCCAGAG′] 2086 NM_001042492.2(NF1): c.2288T > 4763 NF1 [′CTTCAGAAAAGAGTGATGGCACTGCBGAGG C (p.Leu763Pro) CGCATTGAGCATCCCACTGCA′] 2087 NM_004990.3(MARS): c.1108T > 4141 MARS [′CCTCACCAGAATCACCCAGGACATTYTCCAG C (p.Phe370Leu) CAGTTGCTGAAACGAGGTTT′] 2088 NM_004990.3(MARS): c.1568T > 4141 MARS [′TTCTATGTCTGGTTTGATGCCACTAYTGGCT C (p.Ile523Thr) ATCTGTCCATCACAGCCAAC′] 2089 NM_006920.4(SCN1A): c.4729T > −1 — [′GTTCATTGTGCTATTTACTGGAGAGYGTGTA C (p.Cys1577Arg) CTGAAACTCATCTCTCTACG′] 2090 NM_006920.4(SCN1A): c.5113T > −1 — [′TGAGACCTTTGGCAACAGCATGATCYGCCTA C (p.Cys1705Arg) TTCCAAATTACAACCTCTGC′] 2091 NM_006920.4(SCN1A): c.838T > 6323 SCN1A [′CAACCTGAGGAATAAATGTATACAAYGGCC C (p.Trp280Arg) TCCCACCAATGCTTCCTTGGA′] 2092 NM_006920.4(5CN1A): c.269T > 6323 SCN1A [′TGTTGTGTTCCTGTCTTACAGACTTYTATAGT C (p.Phe90Ser) ATTGAATAAAGGGAAGGCC′] 2093 NM_006920.4(5CN1A): c.272T > 6323 SCN1A [′TGTGTTCCTGTCTTACAGACTTTTAYAGTATT C (p.Ile91Thr) GAATAAAGGGAAGGCCATC′] 2094 NM_006920.4(SCN1A): c.3827T > −1 — [′TATTTCACCAATGCCTGGTGTTGGCYGGACT C (p.Leu1276Pro) TCTTAATTGTTGATGTAGGT′] 2095 NM_006920.4(5CN1A): c.5522T > −1 — [′CCAAACAAACTCCAGCTCATTGCCAYGGATT C (p.Met1841Thr) TGCCCATGGTGAGTGGTGAC′] 2096 NM_006920.4(SCN1A): c.568T > 6323 SCN1A [′AGATTTTACTTTCCTTCGGGATCCAYGGAAC C (p.Trp190Arg) TGGCTCGATTTCACTGTCAT′] 2097 NM_002608.2(PDGFB): c.356T > 5155 PDGFB [′ATAGACCGCACCAACGCCAACTTCCYGGTG C (p.Leu119Pro) TGGCCGCCCTGTGTGGAGGTG′] 2098 NM_000833.4(GRIN2A): c.2T > C 2903 GRIN2A [′TTGCAGGGACCGTCAGTGGCGACTAYGGGC (p.Met1Thr) AGAGTGGGCTATTGGACCCTG′] 2099 NM_002474.2(MYH11): c.3791T > 4629 MYH11 [′CTCCCCATCGCTGCACTTGGACTGCDGCTCC C (p.Leu1264Pro) TGCACCTGCGCCTCCAGCTT′] 2100 NM_014139.2(SCN11A): c.2432T > 11280 SCN11A [′CTCAACCTCTTCATTGCCTTACTGCYCAATT C (pLeu811Pro) CCTTTAGCAATGAGGAAAGA′] 2101 NM_006514.3(SCN10A): c.1661T > 6336 SCN10A [′GGAGTCAGGGTTGCTGGGTTGAGGARGAGG C (p.Leu554Pro) GCTTCTAGGGAGGGGGCCTTG′] 2102 NM_000179.2(MSH6): c.1346T > 2956 MSH6 [′CTTATTGGAGTCAGTGAACTGGGGCYGGTAT C (p.Leu449Pro) TCATGAAAGGCAACTGGGCC′] 2103 NM_000179.2(MSH6): c.4001 + 2956 MSH6 [′GAATCAGTCACTACGATTATTTCGGYAACTA 2T > C ACTAACTATAATGGAATTAT′] 2104 NM_000249.3(MLH1): c.1745T > 4292 MLHI [′GCTTCTTCCTAGGAGCCAGCACCGCYCTTTG C (p.Leu582Pro) ACCTTGCCATGCTTGCCTTA′] 2105 NM_000249.3(MLH1): c.2246T > 4292 MLHI [′CTGCAGCTTGCTAACCTGCCTGATCHATACA C (p.Leu749Pro) AAGTCTTTGAGAGGTGTTAA′] 2106 NM_000249.3(MLH1): c.229T > C 4292 MLHI [′ACAGAAAGAAGATCTGGATATTGTAYGTGA (p.Cys77Arg) AAGGTTCACTACTAGTAAACT′] 2107 NM_000249.3(MLH1): c.453 + 2T > 4292 MLHI [′GGCAATCAAGGGACCCAGATCACGGYAAGA C ATGGTACATGGGAGAGTAAAT′] 2108 NM_000249.3(MLH1): c.739T > C 4292 MLHI [′AGCCTTCAAAATGAATGGTTACATABCCAAT (p.Ser247Pro) GCAAACTACTCAGTGAAGAA′] 2109 NM_000249.3(MLH1): c.790 + 2T > 4292 MLHI [′GCATCTTCTTACTCTTCATCAACCGHAAGTT C AAAAAGAACCACATGGGAAA′] 2110 NM_000251.2(MSH2): c.1319T > 4436 MSH2 [′TTGTTGGCAGTTTTTGTGACTCCTCYTACTG C (p.Leu440Pro) ATCTTCGTTCTGACTTCTCC′] 2111 NM_000251.2(MSH2): c.2005 + 2T > 4436 MSH2 [′AACAGATGTTCCACATCATTACTGGYAAAA C AACCTGGTTTTTGGGCTTTGT′] 2112 NM_000251.2(MSH2): c.2089T > 4436 MSH2 [′GGCCCAAATTGGGTGTTTTGTGCCAYGTGAG C (p.Cys697Arg) TCAGCAGAAGTGTCCATTGT′] 2113 NM_000251.2(MSH2): c.560T > C 4436 MSH2 [′CCTGATAATGATCAGTTCTCCAATCBTGAGG (p.Leu187Pro) CTCTCCTCATCCAGATTGGA′] 2114 NM_000251.2(MSH2): c.595T > C 4436 MSH2 [′CCTCATCCAGATTGGACCAAAGGAAYGTGT (p.Cys199Arg) TTTACCCGGAGGAGAGACTGC′] 2115 NM_000251.2(MSH2): c.929T > C 4436 MSH2 [′TTGGATATTGCAGCAGTCAGAGCCCBTAACC (p.Leu310Pro) TTTTTCAGGTAAAAAAAAAA′] 2116 NM_007294.3(BRCA1): c.134 + 2T > 672 BRCAI [′AAAGTGTGACCACATATTTTGCAAGBAAGTT C TGAATGTGTTATGTGGCTCC′] 2117 NM_001194998.1(CEP152): c. 22995 CEP152 [′TATGAGGAAGACATCCTGACTGTACYTGGG 3149T > C (p.Leu1050Pro) GTTCTTTTAAGTGATACCCAA′] 2118 NM_001271723.1(FBX038): c. 81545 FBX038 [′AGGGGTGAATGTTCCTGAAATTCCTYGTATC 616T > C (p.Cys206Arg) CCAATGCTAAGGCACCTTTA′] 2119 NM_014495.3(ANGPTL3): c.883T > −1 — [′ACATCGAATAGATGGATCACAAAACYTCAA C (p.Phe295Leu) TGAAACGTGGGAGAACTACAA′] 2120 NM_003184.3(TAF2): c.1945T > 6873 TAF2 [′CTCTCATAGCGGAGCTGATACTGCCRCATAA C (p.Trp649Arg) AATCAGCTTGCTCAAATTCT′] 2121 NM_000016.5(ACADM): c.233T > 34 ACADM [′TTCTTCTAGTATCCAGTCCCCCTAAYTAGAA C (p.Ile78Thr) GAGCCTGGGAACTTGGTTTA′] 2122 NM_000019.3(ACAT1): c.730 + 2T 38 ACATI [′TTCCTGTCACAGTTACAGTAAAAGGYAGAG C ATAATGTTCCAAAAAGGATGA′] 2123 NM_000143.3(FH): c.1255T > C 2271 FH [′GAAGCATCCCCCAGCAGCCTGGCTGRGTGT (p.Ser419Pro) AACACATTTTTAATCTTTGAG′] 2124 NM_000169.2(GLA): c.899T > C −1 — [′GCTCCTTTATTCATGTCTAATGACCYCCGAC (p.Leu300Pro) ACATCAGCCCTCAAGCCAAA′] 2125 NM_000202.6(IDS): c.587T > C 3423 IDS [GTGCTGGATGTTCCCGAGGGCACCTYGCCTG (p.Leu196Ser) ACAAACAGAGCACTGAGCAA′] 2126 NM_000252.2(MTM1): c.688T > 4534 MTM1 [′TCTGACTTAACCATAGGTGCTGTCAYGGATT C (p.Trp230Arg) CATCCAGAAAATAAGACGGT′] 2127 NM_000277.1(PAH): c.638T > C 5053 PAH [′GAGTACAATCACATTTTTCCACTTCYTGAAA (p.Leu213Pro) AGTACTGTGGCTTCCATGAA′] 2128 NM_000404.2(GLB1): c.457 + 2T > 2720 GLB1 [′TTCTTCTCCGCTCCTCCGACCCAGGYAGGTT C GTTACAGATGTCTTGAGAAG′] 2129 NM_000528.3(MAN2B1): c.2436 + 4125 MAN2B1 [′AGAGATGGCTCGCTGGAGCTCATGGYGAGT 2T > C GGGTCAGAGCCCCATCCGAGC′] 2130 NM_000531.5(OTC): c.540 + 2T > 5009 OTC [′CTGGCTGATTACCTCACGCTCCAGGHTGGTT C TATTTATTTGTCTTACAAAA′] 2131 NM_001005741.2(GBA): c.667T > 2629 GBA [′TTCACCGCTCCATTGGTCTTGAGCCRAGTGG C (p.Trp223Arg) GTGATGTCCAGGGGCTGGCA′] 2132 NM_000402.4(G6PD): c.473T > C 2539 G6PD [′CGCCTCAACAGCCACATGAATGCCCYCCAC (p.Leu158Pro) CTGGGGTCACAGGCCAACCGC′] 2133 NM_000402.4(G6PD): c.188T > C −1 — [′CACCGATGCACCCATGATGATGAATRTGTGT (p.Ile63Thr) GTATCCGACTGATGGAAGGC′] 2134 NM_006920.4(SCN1A): c.4251 + −1 — [′GGGTATCTCTCTTTGCTTCAAGTTGYAAGTG 2T > C AACACTATTTTCTCTGAATA′] 2135 NM_002225.3(IVD): c.465+2T > 3712 IVD [′CAGAAAGAGAAGTATCTCCCGAAGGYGAGG C AAATGGAAATGTAATACACGC′] 2136 NM_003494.3(DYSF): c.1284 + 2T > 8291 DYSF [′GAGGTCAGCTTTGCGGGGAAAATGGYAAGG C AGCAAGGGAGCAGGAGGGTTC′] 2137 NM_004006.2(DMD): c.2380 + 2T > 1756 DMD [′CCCTGGTGGAACAGATGGTGAATGGYAATT C ACACGAGTTGATTTAGATAAT′] 2138 NM_012463.3(ATP6V0A2): c.825 + 23545 ATP6V0A2 [′ACCCGCATCCAGGATCTCTACACTGYGAGTA 2T > C AGCTGGAAGTGGATTGCCTC′] 2139 NM_014795.3(ZEB2): c.73+2T > 9839 ZEB2 [′AAGCCAATCCCAGGAGGAAAAACGGYAAG C AAGCAGCCCGAACCAAACTTTT′] 2140 NM_016725.2(FOLR1): c.493 + 2T > 2348 FOLR1 [′ACAAGGGCTGGAACTGGACTTCAGGYGAGG C GCTGGGGTGGGCAGGAATGGA′] 2141 NM_000056.3(BCKDHB): c.752T > 594 BCKDHB [′CCTGTTCTGTATTTAGCGGAAGAAGYCCCTA C (p.Val251Ala) TAGAACCATACAACATCCCA′] 2142 NM_175053.3(KRT74): c.821T > 121391 KRT74 [′TACTGCATCATACAGACACTTGAGGRACTTG C (p.Phe274Ser) ATTTCTTTGTCCAGTGAGTC′] 2143 NM_003764.3(STX11): c.173T > 8676 STX11 [′GACATTCAGGATGAAAACCAGCTGCYGGTG C (p.Leu58Pro) GCCGACGTGAAGCGGCTGGGA′] 2144 NM_001044.4(SLC6A3): c.671T > 6531 SLC6A3 [′CTCTGCAGACGTGGCGTGCTGCACCYCCACC C (p.Leu224Pro) AGAGCCATGGCATCGACGAC′] 2145 NM_015662.2(IFT172): c.5179T > −1 — [′CTGATGAATTTCAGCACGTCCTGGCRCACTG C (p.Cys1727Arg) GGCTGTGGGAGGTCTGTGAG′] 2146 NM_015662.2(IFT172): c.4607T > 26160 IFT172 [′GCGCGTGGCATAGTAATGAGCGATCRGCAG C (p.Leu1536Pro) CATCGTCTTGAACTCCTCATG′] 2147 NM_014754.2(PTDSS1): c.794T > 9791 PTDSS1 [′ACCGGGAAGATCAAGAGAGCTGTTCYGCAG C (p.Leu265Pro) TTCACTCCTGCTAGCTGGACC′] 2148 NM_014714.3(IFT140): c.4078T > 9742 IFT140 [′GGACCCCAAGGAGTCCATCAAGCAGYGTGA C (p.Cys1360Arg) GCTGCTCCTGGAGGAACCAGA′] 2149 NM_000531.5(OTC): c.1005 + 2T > 5009 OTC [′GAAAACAGAAAGTGGACAATCATGGYAAGC C AAGAAACAAGGAATGGAGGAT′] 2150 NM_000531.5(OTC): c.1018T > C 5009 OTC [′GTTGTGTCATCAGGCTGTCATGGTGYCCCTG (p.Ser340Pro) CTGACAGATTACTCACCTCA′] 2151 NM_000531.5(OTC): c.1022T > C 5009 OTC [′TGTCATCAGGCTGTCATGGTGTCCCYGCTGA (p.Leu341Pro) CAGATTACTCACCTCAGCTC′] 2152 NM_000531.5(OTC): c.1033T > C 5009 OTC [′TGTCATGGTGTCCCTGCTGACAGATBACTCA (p.Tyr345His) CCTCAGCTCCAGAAGCCTAA′] 2153 NM_000531.5(OTC): c.143T > C 5009 OTC [′CGTGACCTTCTCACTCTAAAAAACTYTACCG (p.Phe48Ser) GAGAAGAAATTAAATATATG′] 2154 NM_000531.5(OTC): c.158T > C 5009 OTC [′CTAAAAAACTTTACCGGAGAAGAAABTAAA (p.Ile53Thr) TATATGCTATGGCTATCAGCA′] 2155 NM_000531.5(OTC): c.167T > C 5009 OTC [′TTTACCGGAGAAGAAATTAAATATAYGCTA (p.Met56Thr) TGGCTATCAGCAGATCTGAAA′] 2156 NM_000531.5(OTC): c.188T > C 5009 OTC [′TATATGCTATGGCTATCAGCAGATCYGAAAT (p.Leu63Pro) TTAGGATAAAACAGAAAGGA′] 2157 NM_000531.5(OTC): c.227T > C 5009 OTC [′GTCCTTGATTTATAGTATTTGCCTTYATTGCA (p.Leu76Ser) AGGGAAGTCCTTAGGCATG′] 2158 NM_000531.5(OTC): c.284T > C 5009 OTC [′GAGAAAAGAAGTACTCGAACAAGATYGTCT (p.Leu95Ser) ACAGAAACAGGTAAGTCCACT′] 2159 NM_000531.5(OTC): c.2T > C 5009 OTC [′CGTCCTTTACACAATTAAAAGAAGAYGCTGT (p.Met1Thr) TTAATCTGAGGATCCTGTTA′] 2160 NM_000531.5(OTC): c.386 + 2T > 5009 OTC [′TGAAAGTCTCACGGACACGGCCCGGYTTGT C AAATATTTTCTTCTCTCCAAA′] 2161 NM_000531.5(OTC): c.392T > C 5009 OTC [′TTTTTCTTGGTTTGCCACAGTGTATYGTCTAG (p.Leu131Ser) CATGGCAGATGCAGTATTG′] 2162 NM_000531.5(OTC): c.394T > C 5009 OTC [′TTTCTTGGTTTGCCACAGTGTATTGYCTAGC (p.Ser132Pro) ATGGCAGATGCAGTATTGGC′] 2163 NM_000531.5(OTC): c.416T > C 5009 OTC [HIGTCTAGCATGGCAGATGCAGTATYGGCTC (p.Leu139Ser) GAGTGTATAAACAATCAGAT′] 2164 NM_000531.5(OTC): c.443T > C 5009 OTC [′GCTCGAGTGTATAAACAATCAGATTBGGAC (p.Leu148Ser) ACCCTGGCTAAAGAAGCATCC′] 2165 NM_000531.5(OTC): c.476T > C 5009 OTC [′CTGGCTAAAGAAGCATCCATCCCAAYTATC (p.Ile159Thr) AATGGGCTGTCAGATTTGTAC′] 2166 NM_000531.5(OTC): c.479T > C 5009 OTC [′GCTAAAGAAGCATCCATCCCAATTANCAAT (p.Ile160Thr) GGGCTGTCAGATTTGTACCAT′] 2167 NM_000531.5(OTC): c.490T > C 5009 OTC [′ATCCATCCCAATTATCAATGGGCTGYCAGAT (p.Ser164Pro) TTGTACCATCCTATCCAGAT′] 2168 NM_000531.5(OTC): c.526T > C 5009 OTC [′CCATCCTATCCAGATCCTGGCTGATYACCTC (p.Tyr176His) ACGCTCCAGGTTGGTTTATT′] 2169 NM_000531.5(OTC): c.536T > C 5009 OTC [′CAGATCCTGGCTGATTACCTCACGCYCCAGG (p.Leu179Pro) TTGGTTTATTTATTTGTCTT′] 2170 NM_000531.5(OTC): c.577T > C 5009 OTC [′CTCTCTGAAAGGTCTTACCCTCAGCBGGATC (p.Trp193Arg) GGGGATGGGAACAATATCCT′] 2171 NM_000531.5(OTC): c.602T > C 5009 OTC [′TGGATCGGGGATGGGAACAATATCCYGCAC (p.Leu201Pro) TCCATCATGATGAGCGCAGCG′] 2172 NM_000531.5(OTC): c.663 + 2T > 5009 OTC [′CACCTTCAGGCAGCTACTCCAAAGGYAGGG C AAACTTTTTGCCTTGAAACTA′] 2173 NM_000531.5(OTC): c.779T > C 5009 OTC [′GAAGCAGCGCATGGAGGCAATGTATYAATT (p.Leu260Ser) ACAGACACTTGGATAAGCATG′] 2174 NM_000531.5(OTC): c.793T > C 5009 OTC [′AGGCAATGTATTAATTACAGACACTYGGAT (p.Trp265Arg) AAGCATGGGACAAGAAGAGGA′] 2175 NM_000531.5(OTC): c.803T > C 5009 OTC [′TTAATTACAGACACTTGGATAAGCAYGGGA (p.Met268Thr) CAAGAAGAGGAGAAGAAAAAG′] 2176 NM_000531.5(OTC): c.907T > C 5009 OTC [′TGCCTCTGACTGGACATTTTTACACBGCTTG (p.Cys303Arg) CCCAGAAAGCCAGAAGAAGT′] 2177 NM_000531.5(OTC): c.947T > C 5009 OTC [′CCAGAAGAAGTGGATGATGAAGTCTYTTAT (p.Phe316Ser) TCTCCTCGATCACTAGTGTTC′] 2178 NM_000322.4(PRPH2): c.637T > 5961 PRPH2 [′CCTGGTGGACGGCGTCCCTTTCAGCYGCTGC C (p.Cys213Arg) AATCCTAGCTCGCCACGGCC′] 2179 NM_000322.4(PRPH2): c.736T > 5961 PRPH2 [′CCACCAGACGGAGGAGCTCAACCTGYGGGT C (p.Trp246Arg) GCGTGGCTGCAGGGCTGCCCT′] NM_017415.2(KLHL3): c.1160T > 26249 KLHL3 [] C (p.Leu387Pro) NM_017415.2(KLHL3): c.1280T > 26249 KLHL3 [] C (p.Met427Thr) 2180 NM_003859.1(DPM1): c.742T > C −1 — [′AGAGTCAATAATCCTTTCAAGAAAGRTACTA (p.Ser248Pro) TTTCATTTCCTCCCAACTTG] 2181 NM_004826.3(ECEL1): c.2278T > 9427 ECEL1 [′GGGTTCATGGGTGAGTCCTTGGGACRGTGG C (p.Cys760Arg) AAAGCCCGGCCAAACTCCTCA′] 2182 NM_014908.3(DOLK): c.2T > C 22845 DOLK [′GGCCGGAGATGGGCACTCTCGGGTCRTATCT (p.Met1Thr) CTAGACCTGGGGCTTCACGG′] 2183 NM_000404.2(GLB1): c.922T > C 2720 GLB1 [′TCTTTTGTTTCCTTGTAGGTACATGYTTATAG (p.Phe308Leu) GTGGGACCAATTTTGCCTA′] 2184 NM_012434.4(SLC17A5): c.500T > 26503 SLC17A5 [′TTAGGAGTTGGACCACTCATTGTACYCAGAG C (p.Leu167Pro) CACTAGAAGGACTAGGAGAG′] 2185 NM_000211.4(ITGB2): c.1877 + 3689 ITGB2 [′CCCCTCACCCTGTGGCAAGTACATGYGAGTG 2T > C CAGGCGGAGCAGGCAGGGCG′] 2186 NM_006702.4(PNPLA6): c.3053T > 10908 PNPLA6 [′ACCTCCATGTTCACTGGGTCTGCCTYTAACC C (p.Phe1018Ser) GCAGCATCCATCGGGTCTTC′] 2187 NM_176787.4(PIGN): c.808T > C 23556 PIGN [′TCTGAAGGATGACCAGCCCCATGGGRACCT (p.Ser270Pro) ACAAATAAGATATAAAGAATA′] 2188 NM_001165899.1(PDE4D): c.1850T > 5144 PDE4D [′CCATGTCTCCCAGAGGGGATGAACARTATA C (p.Ile617Thr) GTCTATGAAGCCCACCTAGTT′] 2189 NM_005017.3(PCYT1A): c.571T > 5130 PCYT1A [′CCTTCTGTCCTCTGTGTTGGAGCAARCATGC C (p.Phe191Leu) CTAACTCAGAAACACATACA′] 2190 NM_000090.3(COL3A1): c.951 + 1281 COL3A1 [′CAGGACTTCCTGGGGCTGCAGTGAGHATAG 6T > C (p.Gly300_Ala317del) CTGCTAACATCACACAATTAC′] 2191 NM_000090.3(COL3A1): c.2022 + 1281 COL3A1 [′GCACCTGGAGCTCCAGGAGGCAAGGYAGTA 2T > C (p.Gly660_Lys674del) TTTCAATTTATTCTCTACCTT′] 2192 NM_000090.3(COL3A1): c.951 + 1281 COL3A1 [′CGGCCAGGACTTCCTGGGGCTGCAGHGAGT 2T > C (p.Gly300_Ala317del) ATAGCTGCTAACATCACACAA′] 2193 NM_000090.3(COL3A1): c.3093 + 1281 COL3A1 [′CGAGATGGATCTCCTGGTGGCAAGGBATAA 2T > C (p.Gly1014_Lys1031del) TAAACACATGTGCAATTGATT′] 2194 NM_000090.3(COL3A1): c.2337 + 1281 COL3A1 [′CCAGCTGGCCAGCCTGGAGATAAGGYAACC 2T > C (p.Gly762_Lys779del) CTTAATACTACCTGGATATAA′] 2195 NM_000090.3(COL3A1): c.1761 + 1281 COL3A1 [′TTCCCCGGTCCTAAAGGAAATGATGBGAGTT 2T > C (p.Gly555_Asp587del) CCTTCATTAATTTCTTCAAT′] 2196 NM_000090.3(C0L3A1): c.3039 + 1281 COL3A1 [′CTGGTGAACCTGGAAGAGATGTGAGYAGCA 6T > C GTTTTTATTCAACCAGCCAGG′] (p.Asp1013_Gly1014insVSSSFY STSQ) 2197 NM_000090.3(COL3A1): c.4399T > 1281 COL3A1 [′GGACGTTGGCCCTGTTTGCTTTTTAYAAACC C (p.Ter1467Gln) AAACTCTATCTGAAATCCCA′] 2198 NM_000090.3(COL3A1): c.2553 + 1281 COL3A1 [′CCCCCTGGAGGTTCTGGACCTGCTGYAAGTT 2T > C (p.Gly816_Ala851del) CCTTCCTCTTTCTCTGTCTA′] 2199 NM_001278503.1(STT3A): c.1877T > 3703 STT3A [′TATACTCCAACTGGGGAGTTCCGTGYGGACC C (p.Val626Ala) GTGAAGGTTCTCCAGTGCTG′] 2200 NM_000277.1(PAH): c.691T > C 5053 PAH [′TAACATTCCCCAGCTGGAAGACGTTYCTCAG (p.Ser231Pro) TTCCTGCAGAGTAAGTCCAC′] 2201 NM_007294.3(BRCA1): c.5467 + 672 BRCA1 [′GGACAGAGGACAATGGCTTCCATGGBAAGG 2T > C TGCCTGCATGTACCTGTGCTA′] 2202 NM_020347.3(LZTFL1): c.260T > 54585 LZTFL1 [′ATACCACTTCTCAGCTTGTGCAAACRGCTGT C (p.Leu87Pro) CGCAGAAGTAACACATTGGT′] 2203 NM_016381.5(TREX1): c.530T > 11277 TREX1 [′CGCCAGCCACAGCCCTGGTGCCTGGYGGCA C (p.Val177Ala) CACAATGGTGACCGCTACGAC′] 2204 NM_015474.3(SAMHD1): c.1106T > 25939 SAMHD1 [′TTTGTGTTGATAAGCTCTACGGTGTRAAGAG C (p.Leu369Ser) TTGCGAGTGTGGAACATGTC′] 2205 NM_005654.5(NR2F1): c.755T > 7025 NR2F1 [′CAGATCACCGACCAGGTGTCCCTGCYACGC C (p.Leu252Pro) CTCACCTGGAGCGAGCTGTTC′] 2206 NM_003638.2(ITGA8): c.2982 + 2T > 8516 ITGA8 [′TGCCTATATATATTTAAAGATACTCRCTACT C ATGCTTCCTTCTGGGAGTTT′] 2207 NM_000321.2(RB1): c.2663+2T > 5925 RB1 [′AGGATCAGATGAAGCAGATGGAAGGYAGG C AACCAGTTTTGAATGTTTTCCA′] 2208 NM_000321.2(RB1): c.1472T > C 5925 RB1 [′CATATGTCTTTATTGGCGTGCGCTCYTGAGG (p.Leu491Pro) TTGTAATGGCCACATATAGC′] 2209 NM_015884.3(MBTPS2): c.1391T > 51360 MBTPS2 [′GCTATTGTTAATGCAGTACCCTGCTYTGCTT C (p.Phe464Ser) TGGATGGACAATGGATTCTA′] 2210 NM_001739.1(CA5A): c.697T > C 763 CASA [′TCGGTCAGCGGCGGGGTGGTGAGCGRGCCC (p.Ser233Pro) GCGTAGGTCCAGTAATCCCAG′] 2211 NM_005051.2(QARS): c.169T > C 5859 QARS [′TCCCTGAGTCGGGAGGCCAAGCCATRTAAC (p.Tyr57His) AGGATCCCGGTAGCTTTGTCA′] 2212 NM_005356.4(LCK): c.1022T > C 3932 LCK [′GGCATCAAGTTGACCATCAACAAACYCCTG (p.Leu341Pro) GACATGGCAGCCCAAGTAAGG′] 2213 NM_021803.3(IL21): c.146T > C 59067 IL21 [′TACCAAGTCATTCACATAATTTTTCRGCTGA (p.Leu49Pro) TCAACAATATCTATAAGTTG′] 2214 NM_005861.3(STUB1): c.719T > −1 — [′TGTGGCAAGATCAGCTTTGAGCTGAYGCGG C (p.Met240Thr) GAGCCGTGCATCACGCCCAGT′] 2215 NM_032575.2(GLIS2): c.523T > C 84662 GLIS2 [′TCAGAACACTTCCCATCCTCCGCAGYGTAAC (p.Cys175Arg) CAGCTCTTTGAGCTCCTGCA′] 2216 NM_001101.3(ACTB): c.224T > C 60 ACTB [′CTGAAGTACCCCATCGAGCACGGCAYCGTC (p.Ile75Thr) ACCAACTGGGACGACATGGAG′] 2217 NM_001101.3(ACTB): c.356T > C 60 ACTB [′AACCCCAAGGCCAACCGCGAGAAGAYGACC (p.Met119Thr) CAGGTGAGTGGCCCGCTACCT′] 2218 NM_003401.3(XRCC4): c.127T > 7518 XRCC4 [′TACACTTACTGATGGTCATTCAGCAYGGACT C (p.Trp43Arg) GGGACAGGTAATACTAAAAA′] 2219 NM_000051.3(ATM): c.2638 + 2T > 472 ATM [′CTGGAGAGAGCCAAAGTACCATAGGYAAAT C ACATATTTACTACTTGGGATT′] 2220 NM_000535.5(PMS2): c.2T > C 5395 PMS2 [′CGAGGCGGATCGGGTGTTGCATCCAHGGAG (p.Met1Thr) CGAGCTGAGAGCTCGAGGTGA′] 2221 NM_015713.4(RRM2B): c.190T > 50484 RRM2B [′ATGCCACGTACCTCTTCTGCTGTCCRGAAGG C (p.Trp64Arg) AAGCCTGTGCCTGTTTATAC′] 2222 NM_015713.4(RRM2B): c.368T > 50484 RRM2B [′CTCGATGAGAATTTGAAAGCCATAGRAACA C (p.Phe123Ser) GCGAGCCTCTGGAACCTGCAC′] 2223 NM_003122.4(SPINK1): c.194 + 6690 SPINK1 [′TTAAAAGAAACTCAAGTTTGTACTCRCCGAT 2T > C TTTCAAAACATAACACGCAT′] 2224 NM_003122.4(SPINK1): c.160T > 6690 SPINK1 [′AAACATAACACGCATTCATTGGGATRAGTAT C (p.Tyr54His) TTCCATCAGTCCCACAGACA′] NM_000219.5(KCNE1): c.158T > 3753 KCNE1 [] C (p.Phe53Ser) 2225 NM_000219.5(KCNE1): c.176T > 3753 KCNE1 [′GCGGATGTAGCTCAGCATGATGCCCRGGGT C (p.Leu59Pro) GAAGAAGCCGAAGAATCCCAG′] NM_000219.5(KCNE1): c.259T > 3753 KCNE1 [] C (p.Trp87Arg) 2226 NM_017890.4(VPS13B): c.11119 + 157680 VPS13B [′CGTTTGTAAAGCACATCTCCAAAGGYAGCG 2T > C GGTTCCGTTCCTTGTAATAAT′] 2227 NM_005026.3(PIK3CD): c.1246T > 5293 PIK3CD [′CACCTGCCCTGTCCTTCTGCAGGACYGCCCC C (p.Cys416Arg) ATTGCCTGGGCCAACCTCAT′] 2228 NM_000271.4(NPC1): c.2054T > 4864 NPC1 [′TACATTGGGTTGCCCTTGACCCTCAYTGTGA C (p.Ile685Thr) TTGAAGTCATCCCGTTCCTG′] 2229 NM_002633.2(PGM1): c.1547T > 5236 PGM1 [′GGGAGTGCCGGGGCCACCATTCGGCYGTAC C (p.Leu516Pro) ATCGATAGCTATGAGAAGGAC′] 2230 NM_015599.2(PGM3): c.248T > C 5238 PGM3 [′TTGGTTGATCCTTTGGGTGAAATGTYGGCAC (p.Leu83Ser) CATCCTGGGAGGAACATGCC′] 2231 NM_002136.2(HNRNPA1): c.817T > 3178 HNRNPA1 [′GAATTACAACAATCAGTCTTCAAATBTTGGA C (p.Phe273Leu) CCCATGAAGGGAGGAAATTT′] 2232 NM_002136.2(HNRNPA1): c.841T > 3178 HNRNPA1 [′TTTTGGACCCATGAAGGGAGGAAATYTTGG C (p.Phe281Leu) AGGCAGAAGCTCTGGCCCCTA′] 2233 NM_001159287.1(TPI1): c.833T > 7167 TPI1 [′GGTGGTGCTTCCCTCAAGCCCGAATYCGTGG C (p.Phe278Ser) ACATCATCAATGCCAAACAA′] 2234 NM_130838.1(UBE3A): c.2T > C 7337 UBE3A [′TCTGACGACATTGAAGCTAGCCGAAYGTAA (p.Met1Thr) GTGTAACTTGGTTGAGACTGT′] 2235 NM_130838.1(UBE3A): c.710T > 7337 UBE3A [′ATTGAAACTGCCTTTCTCAATGCACHTGTAT C (p.Leu237Pro) ATTTGTCACCTAACGTGGAA′] 2236 NM_000414.3(HSD17B4): c.1547T > 3295 HSD17B4 [′AGTGGAGACTGGAATCCCTTACACAYTGAT C (p.Ile516Thr) CCTAACTTTGCTAGTCTAGCA′] 2237 NM_022068.3(PIEZO2): c.8215T > 63895 PIEZO2 [′GTCCATTTGATCATTGTCTCTGGTGRGCGAT C (p.Ser2739Pro) ATAGGAATATTAATTTGGCA′] 2238 NM_012079.5(DGAT1): c.751 + 8694 DGAT1 [′GCACCTCAGGCCCACAGAGGTCCTCRCCGC 2T > C GGTAGGTCAGATTGTCCGGGT′] 2239 NM_003108.3(S0X11): c.178T > 6664 SOX11 [′GCCGATGAACGCGTTCATGGTATGGYCCAA C (p.Ser60Pro) GATCGAACGCAGGAAGATCAT′] 2240 NM_021072.3(HCN1): c.814T > C 348980 HCN1 [′TGATGTATGTATCTAATTAACCTTGRAAGTC (p.Ser272Pro) GTAATAAACGCAAGAGACTG′] 2241 NM_001079867.1(PEX2): c.739T > 5828 PEX2 [′CACCAGTGGCAAAGAATGCGCTCTAYGTGG C (p.Cys247Arg) AGAGTGGCCCACCATGCCTCA′] 2242 NM_022552.4(DNMT3A): c.1943T > 1788 DNMT3A [′CTGAATGCCCAAGTCCTTCAGCACCRGGAGC C (p.Leu648Pro) CCTGCACCAGCCAGCAGACA′] 2243 NM_022552.4(DNMT3A): c.2705T > 1788 DNMT3A [′CGCAAAATACTCCTTCAGCGGAGCGRAGAG C (p.Phe902Ser) GTGGCGGATGACTGGCACGCT′] 2244 NM_018400.3(SCN3B): c.482T > 55800 SCN3B [′GAGGAAGACCAGAAGGATGTACATCRTGAT C (p.Met161Thr) TTCTGAGACCACAGAGGTGAA′] 2245 NM_000406.2(GNRHR): c.392T > 2798 GNRHR [′TTCTCCATGTATGCCCCAGCCTTCAYGATGG C (p.Met131Thr) TGGTGATCAGCCTGGACCGC′] 2246 NM_001030001.2(RPS29): c.149T > 6235 RPS29 [′GCAGACGCCTACCTTAATGAAACCGRTATCC C (p.Ile50Thr) TTCGCGTACTGACGGAAACA′] 2247 NM_177550.4(SLC13A5): c.1463T > 284111 SLC13A5 [′CAGGGTACAGGGCAGCATGATGTACRGCGG C (p.Leu488Pro) ATTGAGGCCGATGGAGCGAGA′] 2248 NM_004464.3(FGF5): c.520T > C 2250 FGF5 [′TTTTCAAGAAAATAGCTATAATACCBATGCC (p.Tyr174His) TCAGCAATACATAGAACTGA′] 2249 NM_000051.3(ATM): c.4776 + 2T > 472 ATM [′AGAGGACCCTTTTCACTCTTGGAGGYAATAA C AAATTTCATCATCTACTATT′] 2250 NM_002880.3(RAF1): c.1808T > 5894 RAF1 [′GTGTTGGAGCAGCTCAATGGAAGACRGGAT C (p.Leu603Pro) CTGAAACAAAGCCCAAGAATG′] 2251 NM_000251.2(MSH2): c.942 + 2T > 4436 MSH2 [′GTCAGAGCCCTTAACCTTTTTCAGGBAAAAA C AAAAAAAAAAAAAAAAAAAA′] 2252 NM_001077620.2(PRCD): c.2T > −1 — [′GGGAGGGGATGGGGCAGCTGCGCCAYGTGC C (p.Met1Thr) ACCACCCTTTTCCTGCTCAGC′] 2253 NM_001142800.1(EYS): c.9209T > 346007 EYS [′AGCCACAAAGTTTTTATGTGGATCARTATCC C (p.Ile3070Thr) TCGGAAAGAATTAGACTGTT′] 2254 NM_206933.2(USH2A): c.9751T > 7399 USH2A [′TCCTAAATTGACAGGTGAAGTATGCYGTCCA C (p.Cys3251Arg) GATGAACAGCACAATCGGGT′] 2255 NM_000257.3(MYH7): c.4442T > −1 — [′GAGGCTCGCTCCCTCAGCACAGAGCYCTTCA C (p.Leu1481Pro) AACTCAAGAACGCCTATGAG′] 2256 NM_000257.3(MYH7): c.4835T > −1 — [′GAGACACGCAGCCGCAACGAGGCCCYGAGG C (p.Leu1612Pro) GTGAAGAAGAAGATGGAAGGA′] 2257 NM_000257.3(MYH7): c.4937T > −1 — [′GAGGCCCAGAAGCAAGTCAAGAGCCYCCAG C (p.Leu1646Pro) AGCTTGTTGAAGGTACTCACC′] 2258 NM_001199138.1(NLRC4): c. 58484 NLRC4 [′AATCTCATGAAGACCCCTCTCTTTGYGGTCA 1022T > C (p.Val341Ala) TCACTTGTGCAATCCAGATG′] 2259 NM_000076.2(CDKN1C): c.*5 + 1028 CDKN1C [′GCGCAAGAGGCTGCGGTGAGCCAAGYGAGT 2T > C ACAGCGCACCTGGGGGGGCGC′] 2260 NM_003159.2(CDKL5): c.659T > 6792 CDKL5 [′CCTGGAGAAAGTGAAATTGACCAACYTTTT C (p.Leu220Pro) ACTATTCAGAAGGTGCTAGGA′] 2261 NM_005027.3(PEK3R2): c.1202T > 5296 PIK3R2 [′CTCACCTTCTGCTCCGTTGTGGACCYCATCA C (p.Leu401Pro) ATCACTACCGCCACGAGTCT′] 2262 NM_173596.2(SLC39A5): c.911T > 283375 SLC39A5 [′TTCCTGCTCTTTGTGCTGGAGAACAYGCTGG C (p.Met304Thr) GGCTTTTGCGGCACCGAGGG′] 2263 NM_153334.6(SCARF2): c.190T > 91179 SCARF2 [′CCTTGCTGCCTCCAGCCAGCGCAGCRCGTGG C (p.Cys64Arg) GCACCTGGGAGCTGCGAGCA′] 2264 NM_001288767.1(ARMC5): c.1928T > 79798 ARMC5 [′GGGGCACTTGTGACCGGCCCGGCGCYGTAC C (p.Leu643Pro) GGCCTGCTGACCTATGTGACC′] 2265 NM_001288767.1(ARMC5): c.1379T > 79798 ARMC5 [′CGTGAGGCCATCAACCGGGCCCGACYGCGG C (p.Leu460Pro) GATGCTGGTGGCTTGGATCTA′] 2266 NC_012920.1: m.9478T > C 4514 MT-CO3 [′ATAATCCTATTTATTACCTCAGAAGYTTTTTT CTTCGCAGGATTTTTCTGA′] 2267 NC_012920.1: m.11984T > C 4538 MT-ND4 [′ACTAGTCACAGCCCTATACTCCCTCYACATA TTTACCACAACACAATGGGG′] 2268 NM_014845.5(FIG4): c.50T > C 9896 FIG4 [′ATCAGCTCGGTCCAGAAGCTGGTTCYGTATG (p.Leu17Pro) AGACTAGAGCTGTGAGTACC′] 2269 NM_003159.2(CDKL5): c.145 + 6792 CDKL5 [′TCAAGAAATTCAAGGACAGTGAAGGYAGAT 2T > C ATATATATATATATATATATA′] 2270 NM_002775.4(HTRA1): c.1091T > 5654 HTRA1 [′CCATCTGATAAGATTAAAAAGTTCCYCACG C (p.Leu364Pro) GAGTCCCATGACCGACAGGCC′] 2271 NM_021870.2(FGG): c.1210T > C 2266 FGG [′ATCTTCATAGTGGTTTTCTTCATGGRATACC (p.Ser404Pro) ACCGGGTTTTCCAAGTGGCC′] 2272 NM_017617.3(NOTCH1): c.1285T > 4851 NOTCH1 [′TCGAAGGAGCCCAGCGTGTTGATGCRCTTGC C (p.Cys429Arg) CCGCATGCTCGCAGGGGTTG′] 2273 NM_024915.3(GRHL2): c.1192T > 79977 GRHL2 [′GATGATTCAGATTGACACATACAGTYATAA C (p.Tyr398His) CAATCGTAGCAATAAACCCAT′] 2274 NM_002049.3(GATA1): c.2T > C 2623 GATA1 [′CGCAGGTTAATCCCCAGAGGCTCCAYGGAG (p.Met1Thr) TTCCCTGGCCTGGGGTCCCTG′] 2275 NM_002049.3(GATA1): c.1240T > 2623 GATA1 [′TACTGTGGTGGCTCCGCTCAGCTCAYGAGGG C (p.Ter414Arg) CACAGAGCATGGCCTCCAGA′] 2276 NM_005859.4(PURA): c.299T > C 5813 PURA [′GGCGCGGGCGGCAACAAGAGCCGCCYTACT (p.Leu100Pro) CTCTCCATGTCAGTGGCCGTG′] 2277 NM_032374.4(APOPT1): c.353T > 84334 APOPT1 [′TTCTGGGCAAACCAGAATTTGACTTYTAGTA C (p.Phe118Ser) AGGTAAGTTTAAGTTTTAGA′] 2278 NM_032551.4(KISS1R): c.937T > 84634 KISS1R [′TAAGACCTGGGCTCACTGCATGTCCYACAGC C (p.Tyr313His) AACTCCGCGCTGAACCCGCT′] 2279 NM_017696.2(MCM9): c.1732 + 254394 MCM9 [′AAAGCTTGATACGATTAGCAGAAGGYCTAT 2T > C TTCATTCAGCGAATGATGCTT′] 2280 NM_003159.2(CDKL5): c.602T > 6792 CDKL5 [′GACATGTGGTCGGTGGGCTGTATTCYTGGGG C (p.Leu201Pro) AGCTTAGCGATGGACAGCCT′] 2281 NM_005740.2(DNAL4): c.153 + 10126 DNAL4 [′GAGAAATTCTCCAACAACAACGAGGYATTG 2T > C CCAGCAGTGCAGGCGGCCCCT′] 2282 NM_201631.3(TGM5): c.763T > C 9333 TGM5 [′TTCAGGATGGCCACGCTGCCCGTCCRCTCCG (p.Trp255Arg) CAGGGTTGGCGCCGTCTGTG′] 2283 NM_201631.3(TGM5): c.122T > C 9333 TGM5 [′GCTCCGGTTCCTGAAGTACAGGGTGRGGTTG (p.Leu41Pro) AAGGCCTGGCCCCGGCGAAC′] 2284 NM_001287223.1(SCN11A): c. 11280 SCN11A [′TACCTTCATTCCTTCAAACTGGGACRGCGCA 3473T > C (p.Leu1158Pro) CGAAGAGGCCTCAGTGCTCG′] 2285 NM_001287223.1(SCN11A): c. 11280 SCN11A [′GGGCTCTACTCAGTCTTCTTCTTCAYTGTGG 1142T > C (p.Ile381Thr) TCATTTTCCTGGGCTCCTTC′] 2286 NM_001302946.1(TRNT1): c.668T > 51095 TRNT1 [′CATGATCCTGAGACTTTGGAAGCAAYTGCA C (p.Ile223Thr) GAAAATGCAAAAGGCTTGGCT′] 2287 NM_001302946.1(TRNT1): c.497T > 51095 TRNT1 [′TAATGAATAGGTTTTGATGGCACTTYATTTG C (p.Leu166Ser) ACTACTTTAATGGTTATGAA′] 2288 NM_018136.4(ASPM): c.2419 + 259266 ASPM [′ATAGACACCTATGGAAAGATGTGGGYAAGA 2T > C AGACTGCAGAAATCTTGACAT′] 2289 NM_022455.4(NSD1): c.5885T > 64324 NSD1 [′TGGGGTCTACGGACAAAAACAGATAYTAAA C (p.Ile1962Thr) AAGGTTAGAAAAAGCTAAATT′] 2290 NM_022455.4(NSD1): c.5989T > 64324 NSD1 [′TCAAGAACATGATATCACTAATTTCYATATG C (p.Tyr1997His) CTCACCCTAGACAAAGTAAG′] 2291 NM_133433.3(NIPBL): c.7062 + 25836 NIPBL [′AAAAAATATGCTGGATTCATTCATGYATGTA 2T > C TTTTAACATTTTATAACCTA′] 2292 NM_000525.3(KCNJ11): c.988T > 3767 KCNJ11 [′GGAGGACGGACGTTACTCTGTGGACYACTC C (p.Tyr330His) CAAGTTTGGCAACACCGTCAA′] 2293 NM_004004.5(GJB2): c.107T > C 2706 GJB2 [′CTCTTCATTTTTCGCATTATGATCCYCGTTGT (p.Leu36Pro) GGCTGCAAAGGAGGTGTGG′] 2294 NM_130838.1(UBE3A): c.2485T > 7337 UBE3A [′CTTTAATGTGCTTTTACTTCCGGAAYACTCA C (p.Tyr829His) AGCAAAGAAAAACTTAAAGA′] 2295 NM_005682.6(ADGRG1): c.1460T > 9289 ADGRG1 [′GCCATCTTCCTGCACTTCTCCCTGCYCACCT C (p.Leu487Pro) GCCTTTCCTGGATGGGCCTC′] 2296 NM_000430.3(PAFAH1B1): c.841T > 5048 PAFAH1B1 [′CCGAGAGCATGAGCATGTGGTAGAAYGCAT C (p.Cys281Arg) TTCCTGGGCTCCAGAAAGCTC′] 2297 NM_001005360.2(DNM2): c.1862T > 1785 DNM2 [′GTGGACAGCTGGAAGGCCTCGTTCCYCCGA C (p.Leu621Pro) GCTGGCGTCTACCCCGAGAAG′] 2298 NM_178151.2(DCX): c.683T > C 1641 DCX [′CTGGAGACCGGGGTTGTCAAAAAACYCTAC (p.Leu228Pro) ACTCTGGATGGAAAACAGGTA′] 2299 NM_178151.2(DCX): c.641T > C 1641 DCX [′TCTTTTGAGCAAGTCCTCACTGATAYCACAG (p.Ile214Thr) AAGCCATCAAACTGGAGACC′] 2300 NM_178151.2(DCX): c.412T > C 1641 DCX [′AGACAACTTCTTTAAAAAGGTGGAGYACAC (p.Tyr138His) CAAGAATGTCAATCCCAACTG′] 2301 NM_178151.2(DCX): c.272T > C 1641 DCX [′TTGCTGGCTGACCTGACGCGATCTCYGTCTG (p.Leu91Pro) ACAACATCAACCTGCCTCAG′] 2302 NM_178151.2(DCX): c.128T > C 1641 DCX [′TGTAGCTTCTACCGAACCAGAACCTYGCAG (p.Leu43Ser) GCACTGAGTAATGAGAAGAAA′] 2303 NM_178151.2(DCX): c.2T > C 1641 DCX [′AGGTCTCTGAGGTTCCACCAAAATAYGGAA (p.Met1Thr) CTTGATTTTGGACACTTTGAC′] 2304 NM_000252.2(MTM1): c.260T > 4534 MTM1 [′TCTTCTCTAATACTTGATGTTCCTCYGGGTGT C (p.Leu87Pro) GATCTCGAGAATTGAAAAA′] 2305 NM_000252.2(MTM1): c.683T > 4534 MTM1 [′AACTTTCTGACTTAACCATAGGTGCYGTCAT C (p.Leu228Pro) GGATTCATCCAGAAAATAAG′] 2306 NM_000252.2(MTM1): c.958T > 4534 MTM1 [′TCATAATATTCATGTTATGCGGGAAYCTTTA C (p.Ser320Pro) AAAAAAGTGAAGGACATTGT′] 2307 NM_000252.2(MTM1): c.1353 + 4534 MTM1 [′TGTGTGTGGCAAATGTCAAAACAGGYAAGG 2T > C AATATGAGGGATGAAAATACA′] 2308 NM_000252.2(MTM1): c.1367T > 4534 MTM1 [GTTTTTGTTTAGTTCCCTACAGCTTYTGAATT C (p.Phe456Ser) CAATGAACAATTTTTGATT′] 2309 NM_000252.2(MTM1): c.1433T > 4534 MTM1 [′CTGTATAGTTGCCGATTTGGTACTTYCTTATT C (p.Phe478Ser) CAACTGTGAATCTGCTCGA′] 2310 NM_000252.2(MTM1): c.1495T > 4534 MTM1 [′GGTTACAGAAAGGACTGTTTCTTTAYGGTCA C (p.Trp499Arg) CTGATAAACAGTAATAAAGA′] 2311 NM_006579.2(EBP): c.310T > C 10682 EBP [′TCTCTTCTTTTCTTCAGGGAAAGAGYATGCC (p.Tyr104His) AAGGGAGACAGCCGATACAT′] 2312 NM_152296.4(ATP1A3): c.2270T > 478 ATP1A3 [′GAGCCTGCCTGTGCCACAGGCCGCCYGATCT C (p.Leu757Pro) TCGACAACCTAAAGAAGTCC′] 2313 NM_152296.4(ATP1A3): c.1250T > 478 ATP1A3 [′GTGGCCCTGTCTCACATCGCTGGGCYCTGCA C (p.Leu417Pro) ATCGCGCTGTCTTCAAGGGT′] 2314 NM_152296.4(ATP1A3): c.1144T > 478 ATP1A3 [′GAACCGCATGACAGTCGCCCACATGYGGTT C (p.Trp382Arg) TGACAACCAGATCCACGAGGC′] 2315 NM_152296.4(ATP1A3): c.1112T > 478 ATP1A3 [′ATCTGCTCAGATAAGACAGGGACCCYCACT C (p.Leu371Pro) CAGAACCGCATGACAGTCGCC′] 2316 NM_004963.3(GUCY2C): c.2782T > −1 — [′CTTTCTGTCCTTCATTTCAGGTCCCYGTGCTG C (p.Cys928Arg) CTGGAGTTGTGGGAATCAA′] 2317 NM_000109.3(DMD): c.1700T > 1756 DMD [′GTTCACTGCATCTTCTTTTTCTGAARGCCATG C (p.Leu567Pro) CACTAAAAAGGCACTGCAA′] 2318 NM_001543.4(NDST1): c.1918T > 3340 NDST1 [′CAGCAACTACCCCAGCTCTGAGACCYTTGA C (p.Phe640Leu) GGAGATCCAGTTTTTTAATGG′] 2319 NM_001999.3(FBN2): c.3740T > 2201 FBN2 [′GGTGTCACAGCCTCCGTTCATTATCRTACAT C (p.Met1247Thr) TCATCAATATCTGTGAAAAC′] 2320 NM_005154.4(USP8): c.2152T > 9101 USP8 [′ACCTTCCAAACTGAAGCGCTCCTACYCCTCC C (p.Ser718Pro) CCAGATATAACCCAGGCTAT′] 2321 NM_000734.3(CD247): c.2T > C 919 CD247 [′CCTCTTTCTGAGGGAAAGGACAAGAYGAAG (p.Met1Thr) TGGAAGGCGCTTTTCACCGCG′] 2322 NM_000663.4(ABAT): c.1433T > 18 ABAT [′AAATCCATTCGTTTCCGTCCCACGCYGGTCT C (p.Leu478Pro) TCAGGGATCACCACGCTCAC′] 2323 NM_005211.3(CSF1R): c.2717T > 1436 CSF1R [′ACCCACAGACCCACCTTCCAGCAGAYCTGCT C (p.Ile906Thr) CCTTCCTTCAGGAGCAGGCC′] 2324 NM_005211.3(CSF1R): c.2566T > 1436 CSF1R [′CTTGGCCTTTGCAGGGCTGAATCCCYACCCT C (p.Tyr856His) GGCATCCTGGTGAACAGCAA′] 2325 NM_005211.3(CSF1R): c.2480T > 1436 CSF1R [′GTGAAGTGGATGGCCCCAGAGAGCAYCTTT C (p.Ile827Thr) GACTGTGTCTACACGGTTCAG′] 2326 NM_005211.3(CSF1R): c.2450T > 1436 CSF1R [′TGGGGACTGTCATCCCAGGCCCGCCYGCCTG C (p.Leu817Pro) TGAAGTGGATGGCCCCAGAG′] 2327 NM_005211.3(CSF1R): c.1957T > 1436 CSF1R [′GAACATCGTCAACCTTCTGGGAGCCYGTACC C (p.Cys653Arg) CATGGAGGTAAGGGCCTTGG′] 2328 NM_005211.3(CSF1R): c.1745T > 1436 CSF1R [′AAGTGGGAGTTCCCCCGGAACAACCYGCAG C (p.Leu582Pro) TTTGGTGAGATGGCAGCTCAT′] 2329 NM_001563.3 (EVIPG1): c.461T > 3617 EVIPG1 [′AATTCCCAGGAGCACCTGGATCTTCYCCAGC C (p.Leu154Pro) AGGTGAGCCTAAACACCACA′] NM_001130823.1(DNMT1): c.1531T > 1786 DNMT1 [] C (p.Tyr511His) 2330 NM_152515.4(CKAP2L): c.2T > C 150468 CKAP2L [′AGCAGCGGTAGGCCCGGGCCCCACCRTGAC (p.Met1Thr) TCTTCAGTGACAGTTTTTCTT′] 2331 NM_170707.3(LMNA): c.1968 + 4000 LMNA [′GGCAACTCCAGCCCCCGAACCCAGGNGAGT 2T > C TGTCTCTGCTTTGTCTCCAAA′] 2332 NM_153818.1(PEX10): c.890T > 5192 PEX10 [′AGAAACCCCCTGTGCACCCTGTGCCYGGAG C (p.Leu297Pro) GAGCGCAGGCACCCAACAGCC′] 2333 NM_153818.1(PEX10): c.2T > C 5192 PEX10 [′TCGGGACCACCCGAACCCGCGGCCAYGGCC (p.Met1Thr) CCGGCCGCCGCCAGCCCCCCG′] 2334 NM_014305.3(TGDS): c.700T > C 23483 TGDS [′AATGCTTCTACAACATCAGTAGCATRAAGG (p.Tyr234His) AAGTTTCTTGTTTGAAGCCCT′] 2335 NM_000256.3(MYBPC3): c.2994 + 4607 MYBPC3 [′GTGAACCTTCTCATCCCTTTCCAGGYGGGAC 2T > C TGGCCCCCTTCCCTGTCCCC′] 2336 NM_000256.3(MYBPC3): c.1624 + 4607 MYBPC3 [′CTCATGTGCCCCCCCAGCCAGGCTCDCCCTG 2T > C CACAATGAGCTCAGCCAGCG′] 2337 NM_000257.3(MYH7): c.2207T > 4625 MYH7 [′GCGGCCATCCCTGAGGGACAGTTCAHTGAT C (p.Ile736Thr) AGCAGGAAGGGGGCAGAGAAG′] 2338 NM_000138.4(FBN1): c.7754T > 2200 FBN1 [′TGCCAGCATGGCTGCCAGAACATCAYTGGG C (p.Ile2585Thr) GGCTACAGGTGCAGCTGCCCC′] 2339 NM_000371.3(TTR): c.190T > C 7276 TTR [′GGCTGCTGATGACACCTGGGAGCCAYTTGC (p.Phe64Leu) CTCTGGGTAAGTTGCCAAAGA′] 2340 NM_000126.3(ETFA): c.2T > C 2108 ETFA [′GAGGTTGCGGCGGAAGCGGAGACCAYGTTC (p.Met1Thr) CGAGCGGCGGCTCCGGGGCAG′] 2341 NM_006493.2(CLN5): c.2T > C 1203 CLN5 [′GCGGGCCGGGCGCGGGGAGGTGTCAYGCGC (p.Met1Thr) CGGAACCTGCGCTTGGGGCCA′] 2342 NM_000543.4(SMPD1): c.475T > 6609 SMPD1 [′CTCAGTGCTGAGCCCATCTGAGGCCYGTGGC C (p.Cys159Arg) CTGCTCCTGGGCTCCACCTG′] 2343 NM_000501.3(ELN): c.889 + 2T > 2006 ELN [′TTCCTGGAATTGGAGGCATCGCAGGYAACA C TCTGTCCCAGCAGGGGGCGGG′] 2344 NM_000169.2(GLA): c.758T > C −1 — [′TGGACATCTTTTAACCAGGAGAGAAYTGTTG (p.Ile253Thr) ATGTTGCTGGACCAGGGGGT′] 2345 NM_152743.3(BRAT1): c.176T > 221927 BRAT1 [′GAGCACCCCTGCCTGGTGGAGCTGCYGTCCC C (p.Leu59Pro) ATGTGCTGAAAGTCCAGGAC′] 2346 NM_000152.3(GAA): c.896T > C 2548 GAA [′CTCTACGGGTCTCACCCTTTCTACCBGGCGC (p.Leu299Pro) TGGAGGACGGCGGGTCGGCA′] 2347 NM_015384.4(NIPBL): c.7637T > 25836 N1PBL [′AATGTGTCCCAGGGTATTTTATTACYTCTCA C (p.Leu2546Pro) TGTTAAAACAACATTTGAAG′] 2348 NM_005641.3(TAF6): c.212T > C 6878 TAF6 [′ATTCTTTAGCTTCAAGGCGTAGTCADTGTCA (p.Ile71Thr) CTGGTGGTGAGCTTCTGCCG′] 2349 NM_170707.3(LMNA): c.710T > 4000 LMNA [′ATTGACAATGGGAAGCAGCGTGAGTYTGAG C (p.Phe237Ser) AGCCGGCTGGCGGATGCGCTG′] 2350 NM_000527.4(LDLR): c.1468T > 3949 LDLR [′GGACTGGATCCACAGCAACATCTACYGGAC C (p.Trp490Arg) CGACTCTGTCCTGGGCACTGT′] 2351 NM_000081.3(LYST): c.772T > C 1130 LYST [′CATGAACAATTCTCCATTTGACTTAYGTCAT (p.Cys258Arg) GTTTTGTTATCTTTATTAGA′] 2352 NM_000256.3(MYBPC3): c.3796T > 4607 MYBPC3 [′CTTACAGGGCGAGGCACGGTGTGAGYGCCG C (p.Cys1266Arg) CCTGGAGGTGCGAGGTGAGGA′] 2353 NM_000256.3(MYBPC3): c.3713T > 4607 MYBPC3 [′TTCAGCAAGCAGGGAGTGTTGACTCYGGAG C (p.Leu1238Pro) ATTAGAAAGCCCTGCCCCTTT′] 2354 NM_000256.3(MYBPC3): c.3330 + 4607 MYBPC3 [′CAGAAAGCCGACAAGAAGACCATGGBGAGC 2T > C CCAGGGTCTGGGGTCCCCACG′] 2355 NM_000256.3(MYBPC3): c.1696T > 4607 MYBPC3 [′CGCAAAGGACCAGGCGGTGTTCAAAYGTGA C (p.Cys566Arg) GGTCTCAGATGAGAATGTTCG′] 2356 NM_000256.3(MYBPC3): c.1456T > 4607 MYBPC3 [′ATCGGAGGAGGGGGCGCAAGTCAAABGGTG C (p.Trp486Arg) AGTTCCAGAAGCACGGGGCAT′] 2357 NM_000256.3(MYBPC3): c.709T > 4607 MYBPC3 [′TGCCCAGCCTGCCTTCACTGGCAGCYACCGC C (p.Tyr237His) TGTGAGGTGTCCACCAAGGA′] 2358 NM_000256.3(MYBPC3): c.467T > 4607 MYBPC3 [′GGAGCCCCCGATGACCCCATTGGCCYCTTCG C (p.Leu156Pro) TGATGCGGCCACAGGATGGC′] 2359 NM_000257.3(MYH7): c.2723T > 4625 MYH7 [′GATGCTGAGGAGCGCTGTGATCAGCYGATC C (p.Leu908Pro) AAAAACAAGATTCAGCTGGAG′] 2360 NM_000257.3(MYH7): c.2479T > 4625 MYH7 [′TCGGGCCTTCATGGGGGTCAAGAATYGGCC C (p.Trp827Arg) CTGGATGAAGCTCTACTTCAA′] 2361 NM_000257.3(MYH7): c.1400T > 4625 MYH7 [′GTCCTGGACATCGCTGGCTTCGAGAYCTTCG C (p.Ile467Thr) ATGTGAGTTGGGACCCCTGG′] 2362 NM_000257.3(MYH7): c.1048T > 4625 MYH7 [′CACTTCAGAGGAGAAAAACTCCATGYATAA C (p.Tyr350His) GCTGACAGGCGCCATCATGCA′] 2363 NM_000257.3(MYH7): c.730T > 4625 MYH7 [′CGTCCGGAACGACAACTCCTCCCGCYTCGTG C (p.Phe244Leu) AGTGGTCCCTGACCTTGGCC′] 2364 NM_005159.4(ACTC1): c.755T > −1 — [′CTGCCTGATGGCCAAGTCATCACTAYTGGCA C (p.Ile252Thr) ATGAGCGCTTCCGCTGTCCT′] 2365 NM_030662.3(MAP2K2): c.169T > 5605 MAP2K2 [′GCAGCAGAAGAAGCGGCTGGAAGCCNTTCT C (p.Phe57Leu) CACCCAGAAAGCCAAGGTCGG′] 2366 NM_000169.2(GLA): c.41T > C −1 — [′CCAGAACTACATCTGGGCTGCGCGCYTGCG (p.Leu14Pro) CTTCGCTTCCTGGCCCTCGTT′] 2367 NM_002294.2(LAMP2): c.864 + 3920 LAMP2 [′TATCTAGACTTTGTCTTTGCTGTGGYGAGTA 2T > C ACAGATTTTTTAAAGTTAGG′] 2368 NM_000551.3(VHL): c.227T > C 7428 VHL [′TCGCGCGAGCCCTCCCAGGTCATCTYCTGCA (p.Phe76Ser) ATCGCAGTCCGCGCGTCGTG′] 2369 NM_000551.3(VHL): c.266T > C 7428 VHL [′CCGCGCGTCGTGCTGCCCGTATGGCHCAACT (p.Leu89Pro) TCGACGGCGAGCCGCAGCCC′] 2370 NM_000551.3(VHL): c.473T > C 7428 VHL [′GTTTTTGCCCTTCCAGTGTATACTCHGAAAG (p.Leu158Pro) AGCGATGCCTCCAGGTTGTC′] 2371 NM_000051.3(ATM): c.8584 + 2T > −1 — [′GCAGTGTAGCTACTTCTTCTATTGGYAATCT C TCTTGTACATATAGTAGATT′] 2372 NM_058216.2(RAD51C): c.404 + 5889 RAD51C [′AGGTGTTGGAAAAACACAATTATGGYAAAA 2T > C TAAAGTGTTCTCCTTTTAAGG′] 2373 NM_000455.4(STK11): c.545T > 6794 STK11 [′CACAAGGACATCAAGCCGGGGAACCYGCTG C (p.Leu182Pro) CTCACCACCGGTGGCACCCTC′] 2374 NM_001199107.1(TBC1D24): c. 57465 TBC1D24 [′GGACAACACGCAGGTGCCCAGCTACYGCCT 313T > C (p.Cys105Arg) GAATGCACGCGGCGAGGGGGC′] 2375 NM_000899.4(KITLG): c.98T > C 4254 KITLG [′ACTGAAGGGATCTGCAGGAATCGTGYGACT (p.Val33Ala) AATAATGTAAAAGACGTCACT′] 2376 NM_001195129.1(PRSS56): c.1183T > 646960 PRSS56 [′GCAGTGCCTGCAGCGCCGGCGGCGAYGCGG C (p.Cys395Arg) TCAGTTCTGTTCACCCGGACC′] 2377 NM_006888.4(CALM1): c.268T > 801 CALM1 [′TAGTGAAGAAGAAATCCGTGAGGCAYTCCG C (p.Phe90Leu) AGTCTTTGACAAGGTAATCCA′] 2378 NM_017534.5(MYH2): c.5609T > −1 — [′GAAGATAGAAAGAATATTCTCAGGCYTCAA C (p.Leu1870Pro) GATTTGGTAGATAAACTTCAG′] 2379 NM_003000.2(SDHB): c.574T > C 6390 SDHB [′CTACGAGTGCATTCTCTGTGCCTGCYGTAGC (p.Cys192Arg) ACCAGCTGCCCCAGCTACTG′] 2380 NM_000314.6(PTEN): c.406T > C 5728 PTEN [′AAAGGGACGAACTGGTGTAATGATAYGTGC (p.Cys136Arg) ATATTTATTACATCGGGGCAA′] 2381 NM_000051.3(ATM): c.2T > C 472 ATM [′ATGTGTGTTCTGAAATTGTGAACCAYGAGTC (p.Met1Thr) TAGTACTTAATGATCTGCTT′] 2382 NM_052867.2(NALCN): c.1526T > 259232 NALCN [′GGTCCTGGAAAAAAGCTTGGGAGTTYGGTT C (p.Leu509Ser) GTATTTACTGCCAGCCTCTTG′] 2383 NM_000314.6(PTEN): c.545T > C 5728 PTEN [′TATGTGTATTATTATAGCTACCTGTYAAAGA (p.Leu182Ser) ATCATCTGGATTATAGACCA′] 2384 NM_005957.4(MTHFR): c.1969T > 4524 MTHFR [′ACGCAGGGCGTCAGGACGCAGGGTCRTGGA C (p.Ter657Arg) GCCTCCGTTTCTCTCGCATTC′] 2385 NM_005957.4(MTHFR): c.1883T > 4524 MTHFR [′AACCTGGTGGACAATGACTTCCCACYGGAC C (p.Leu628Pro) AACTGCCTCTGGCAGGTGGTG′] 2386 NM_005957.4(MTHFR): c.1793T > 4524 MTHFR [′CTGTGGATTGAGCGGTGGGGAAAGCYGTAT C (p.Leu598Pro) GAGGAGGAGTCCCCGTCCCGC′] 2387 NM_005957.4(MTHFR): c.1530 + 4524 MTHFR [′AGCGGGGGCTATGTCTTCCAGAAGGYGTGG 2T > C TAGGGAGGCACGGGGTGCCCC′] 2388 NM_005957.4(MTHFR): c.388T > 4524 MTHFR [′CCTGGAGACCATCCTGCACATGACCYGCTGC C (p.Cys130Arg) CGTCAGCGCCTGGAGGAGAT′] 2389 NM_000264.3(PTCH1): c.3168 + 5727 PTCH1 [′AACCCCTGGACGGCCGGGATCATTGYGAGT 2T > C GTATTATAAGGGGCTTTGTGG′] 2390 NM_000030.2(AGXT): c.302T > C 189 AGXT [′GTGCTGGAGCCTGGGGACTCCTTCCYGGTTG (p.Leu101Pro) GGGCCAATGGCATTTGGGGG′] 2391 NM_000030.2(AGXT): c.322T > C 189 AGXT [′CTTCCTGGTTGGGGCCAATGGCATTYGGGGG (p.Trp108Arg) CAGCGAGCCGTGGACATCGG′] 2392 NM_000023.2(SGCA): c.371T > C 6442 SGCA [′ACTCGGCAGAGGCTGGTGCTGGAGAYTGGG (p.Ile124Thr) GACCCAGAAGGTACCTCTAGC′] 2393 NM_016035.4(COQ4): c.155T > C 51117 COQ4 [′CGCCATCGCCGCGGAGCCGGCGGCCRACAG (p.Leu52Ser) CCCTTTCTGCAGCGGGGAGGT′] 2394 NM_000510.2(FSHB): c.298T > C 2488 FSHB [′GTATACATACCCAGTGGCCACCCAGYGTCA (p.Cys100Arg) CTGTGGCAAGTGTGACAGCGA′] 2395 NM_007374.2(SIX6): c.110T > C 4990 SIX6 [′CGCCTGGGTCGCTTCCTCTGGTCGCYGCCCG (p.Leu37Pro) TGGCCCCTGCGGCCTGCGAG′] 2396 NM_000314.6(PTEN): c.202T > C 5728 PTEN [′AAAGCATAAAAACCATTACAAGATAHACAA (p.Tyr68His) TCTGTAAGTATGTTTTCTTAT′] 2397 NM_001103.3(ACTN2): c.683T > 88 ACTN2 [′GAGAAGCACCTGGATATTCCTAAAAYGTTG C (p.Met228Thr) GATGCTGAAGGTGAGATGAAA′] 2398 NM_005249.4(FOXG1): c.700T > 2290 FOXG1 [′GCAGAACTCCATCCGCCACAATCTGYCCCTC C (p.Ser234Pro) AACAAGTGCTTCGTGAAGGT′] 2399 NM_001165963.1(SCN1A): c.4055T > −1 — [′ATTCCATCCATCATGAATGTGCTTCYGGTTT C (p.Leu1352Pro) GTCTTATATTCTGGCTAATT′] 2400 NM_001165963.1(SCN1A): c.2690T > 6323 SCN1A [′GCTCTGGGAAATTTAACCCTCGTCTYGGCCA C (p.Leu897Ser) TCATCGTCTTCATTTTTGCC′] 2401 NM_001165963.1(SCN1A): c.1265T > 6323 SCN1A [′CTAATAAATTTGATCCTGGCTGTGGHGGCCA C (p.Val422Ala) TGGCCTACGAGGAACAGAAT′] 2402 NM_001165963.1(SCN1A): c.1033T > 6323 SCN1A [′TTTCTTTTACCCCACTTGCAGCCAAYGTCCA C (p.Cys345Arg) GAGGGATATATGTGTGTGAA′] 2403 NM_001165963.1(SCN1A): c.769T > 6323 SCN1A [′AGATGTAATGATCCTGACTGTGTTCYGTCTG C (p.Cys257Arg) AGCGTATTTGCTCTAATTGG′] 2404 NM_001165963.1(SCN1A): c.323T > 6323 SCN1A [′TTCCGGTTCAGTGCCACCTCTGCCCYGTACA C (p.Leu108Pro) TTTTAACTCCCTTCAATCCT′] 2405 NM_000426.3(LAMA2): c.8282T > 3908 LAMA2 [′GCAGAATCAGAACCAGCTCTTTTGAYAGGG C (p.Ile2761Thr) AGCAAGCAGTTCGGGCTTTCA′] 2406 NM_001199.3(BMP1): c.*241T > 649 BMP1 [′GTCTGTGACATTTCCTGTTGTGAAGYAAAAG C AGGGACCCCTGCGTCCTGCT′] 2407 NM_001987.4(ETV6): c.1046T > 2120 ETV6 [′CTTTGGGATTACGTCTATCAGTTGCYTTCTG C (p.Leu349Pro) ACAGCCGGTACGAAAACTTC′] 2408 NM_004974.3(KCNA2): c.788T > 3737 KCNA2 [′ATGAACATCATTGACATTGTGGCCAYCATCC C (p.Ile263Thr) CCTACTTCATCACCCTGGGG′] 2409 NM_002055.4(GFAP): c.791T > C 2670 GFAP [′GTGTCCCCCACCTAGTTTGCAGACCYGACAG (p.Leu264Pro) ACGCTGCTGCCCGCAACGCG′] 2410 NM_002055.4(GFAP): c.239T > C 2670 GFAP [′GAGATGATGGAGCTCAATGACCGCTYTGCC (p.Phe80Ser) AGCTACATCGAGAAGGTTCGC′] 2411 NM_002055.4(GFAP): c.218T > C 2670 GFAP [′ACCCGGGCCAGTGAGCGGGCAGAGADGATG (p.Met73Thr) GAGCTCAATGACCGCTTTGCC′] 2412 NM_001288953.1(TTC7A): c.1912T > 57217 TTC7A [′TTTGCCTGATGCCCATGATGCAGACYCTGGT C (p.Ser638Pro) AAGAACGAGCTCCTTGGGCC′] 2413 NM_000257.3(MYH7): c.5117T > −1 — [′TCCCGGAAGCTGGCGGAGCAGGAGCYGATT C (p.Leu1706Pro) GAGACTAGTGAGCGGGTGCAG′] 2414 NM_000257.3(MYH7): c.4772T > −1 — [′ATGGAACAGGCCAAGCGCAACCACCWGCG C (p.Leu1591Pro) GGTGGTGGACTCGCTGCAGACC′] 2415 NM_016218.2(POLK): c.609T > C 51426 POLK [′TGAGTCTTGATGAAGCCTACTTGAAYATAAC (p.Asn203=) AAAGCACTTAGAAGAAAGAC′] 2416 NM_016218.2(POLK): c.*66T > C 51426 POLK [′TATTTTATAATCAATGAATTTGTTCYTTCTGA TTTTAAGTTTGCAGATTTA′] 2417 NM_015662.2(1FT172): c.770T > 26160 1FT172 [′AGTCCTGGGGGCCAGTCTGTTGTGCYAGGA C (p.Leu257Pro) AGTTATGACAGGTAAGTCCCC′] 2418 NM_178454.4(DRAM2): c.79T > 128338 DRAM2 [′GACATCTGCTGCTTTCATATTTTCAYACATT C (p.Tyr27His) ACTGCAGTAACACTCCACCA′] 2419 NM_032790.3(ORAI1): c.581T > 84876 ORAI1 [′CTCTTCCTAGCTGAGGTGGTGCTGCYCTGCT C (p.Leu194Pro) GGGTCAAGTTCTTGCCCCTC′] 2420 NM_001135669.1(XPR1): c.434T > 9213 XPR1 [′GAGTTCTACCTCAGTCTAATCCTGCYGCAGA C (p.Leu145Pro) ACTATCAGGTACTTAGATTC′] 2421 NM_001135669.1(XPR1): c.419T > 9213 XPR1 [′AAACTGGCCTTCAGTGAGTTCTACCYCAGTC C (p.Leu140Pro) TAATCCTGCTGCAGAACTAT′] 2422 NM_001135669.1(XPR1): c.653T > 9213 XPR1 [′GACAGACAAAAGGCTATGAAGCGTTYACGT C (p.Leu218Ser) GTCCCCCCTTTGGGAGCTGCT′] 2423 NM_005859.4(PURA): c.218T > C 5813 PURA [CGGGTGGACATCCAGAACAAGCGCTYCTAC (p.Phe73Ser) CTGGACGTGAAGCAGAACGCC′] 2424 NM_005859.4(PURA): c.563T > C 5813 PURA [′CTGGGCTCCACGCAGGGCCAGACCAYTGCG (p.Ile 188Thr) CTGCCCGCGCAGGGGCTCATC′] 2425 NM_001399.4(EDA): c.396+2T > 1896 EDA [′TCTGACTCCCAGGACGGGCACCAGGKGAGT C CACCTAGTAGGGGCGGCGGCG′] 2426 NM_001848.2(COL6A1): c.957 + 1291 COL6A1 [′TCCAGGGGACCCAAGGGCTACAAGGYGAGC 2T > C GTGGGCTGCTGGGAGGGGGGA′] 2427 NM_000454.4(SOD1): c.341T > C 6647 SOD1 [′TCACTCTCAGGAGACCATTGCATCAYTGGCC (p.Ile114Thr) GCACACTGGTGGTAAGTTTT′] 2428 NM_020451.2(SEPN1): c.872 + 2T > 57190 SEPN1 [′CTTCTACTACACTGTGATGTTCCGGYGAGTG C GGCCACACTGGCTGGCCTGG′] 2429 NM_004453.3 (ETFDH): c.1001T > 2110 ETFDH [′GGTCTAGACTATCAGAATCCATACCYGAGTC C (p.Leu334Pro) CATTTAGAGAGTTCCAAAGG′] 2430 NM_198056.2(SCN5A): c.4299 + 6331 SCN5A [′CAGCTGTGGACTCCAGGGGGGTAGGYTGCC 6T > C ACAGTGGCTTCTTCCACCAAG′] 2431 NM_198056.2(SCN5A): c.2291T > 6331 SCN5A [′TTCACAGGGATTTTCACAGCAGAGABGACCT C (p.Met764Thr) TCAAGATCATTGCCCTCGAC′] 2432 NM_198056.2(SCN5A): c.2047T > 6331 SCN5A [′AGAGTTAGAGGAGTCTCGCCACAAGBGTCC C (p.Cys683Arg) ACCATGCTGGAACCGTCTCGC′] 2433 NM_004415.2(DSP): c.4961T > C 1832 DSP [′GAAAAGCAGAGGACCCAGGAAGAGCYGAG (p.Leu1654Pro) GAGGCTCTCTTCTGAGGTCGAG′] 2434 NM_000238.3(KCNH2): c.2396T > 3757 KCNH2 [′CGGGGCGACGTCGTCGTGGCCATCCYGGGT C (p.Leu799Pro) ATGGGGTGGGGGGCGGGCACT′] 2435 NM_000238.3(KCNH2): c.2366T > 3757 KCNH2 [′TTCATCTCCCGGGGCTCCATCGAGAYCCTGC C (p.Ile789Thr) GGGGCGACGTCGTCGTGGCC′] 2436 NM_000238.3(KCNH2): c.1945 + 3757 KCNH2 [′CTGCGTCATGCTCATTGGCTGTGAGYGTGCC 6T > C CAGGGGCGGGCGGCGGGGAG′] 2437 NM_000238.3(KCNH2): c.1918T > 3757 KCNH2 [′TCCCAACACCAACTCAGAGAAGATCBTCTCC C (p.Phe640Leu) ATCTGCGTCATGCTCATTGG′] 2438 NM_000238.3(KCNH2): c.1282T > 3757 KCNH2 [′CTACACGGCTGTCTTCACACCCTACYCGGCT C (p.Ser428Pro) GCCTTCCTGCTGAAGGAGAC′] 2439 NM_000238.3(KCNH2): c.125T > 3757 KCNH2 [′GCTCGGGTGGAGAACTGCGCCGTCAHCTAC C (p.Ile42Thr) TGCAACGACGGCTTCTGCGAG′] 2440 NM_000218.2(KCNQ1): c.1025T > 3784 KCNQ1 [′GTCTTTGCCATCTCCTTCTTTGCGCYCCCAGC C (p.Leu342Pro) GGTAGGTGCCCCGTGGGTG′] 2441 NM_000218.2(KCNQ1): c.1251 + 3784 KCNQ1 [′CCCAAACCCAAGAAGTCTGTGGTGGYGAGT 2T > C AGCCCACCTGCCACCAGGGCA′] 2442 NM_130799.2(MEN1): c.547T > C 4221 MEN1 [′CCTCGCCCTGTCTGAGGATCATGCCYGGGTA (p.Trp183Arg) GTGTTTGGGCCCAATGGGGA′] 2443 NM_000138.4(FBN1): c.7531T > 2200 FBN1 [′CACCATTGGCGGCTTCACATGCAAAYGTCCT C (p.Cys2511Arg) CCCGGATTTACCCAACACCA′] 2444 NM_000138.4(FBN1): c.7111T > 2200 FBN1 [′ATGCTGCTGTGACGGAGGGAGAGGCYGGGG C (p.Trp2371Arg) TCCCCACTGTGAGATCTGCCC′] 2445 NM_000138.4(FBN1): c.6274T > 2200 FBN1 [′CTGCTGTGCCTTGAAGGGAGAAGGCYGGGG C (p.Trp2092Arg) AGACCCCTGCGAGCTCTGCCC′] 2446 NM_000138.4(FBN1): c.5746T > 2200 FBN1 [′CACAATTGGTTCCTTCAACTGCCGCYGCAAT C (p.Cys1916Arg) CATGGTTTCATCCTTTCTCA′] 2447 NM_000138.4(FBN1): c.5726T > 2200 FBN1 [′GGGAATGGAACTTGCCGGAACACAAYTGGT C (p.Ile1909Thr) TCCTTCAACTGCCGCTGCAAT′] 2448 NM_000138.4(FBN1): c.1468 + 2T > 2200 FBN1 [′TGGACCTCCGTGGGGAGTGTATTGGYACGT C GATCCATCCTAGGTTGGCACC′] 2449 NM_004572.3(PKP2): c.2386T > 5318 PKP2 [′TCTCATTGAAACTACAGCCTCTGCCYGTTAC C (p.Cys796Arg) ACATTGAACAACATAATCCA′] 2450 NM_004333.4(BRAF): c.1783T > 673 BRAF [′AGACCTCACAGTAAAAATAGGTGATYTTGG C (p.Phe595Leu) TCTAGCTACAGTGAAATCTCG′] 2451 NM_000543.4(SMPD1): c.416T > 6609 SMPD1 [′GCCGTGTGCCAATCCATTGTCCACCYCTTTG C (p.Leu139Pro) AGGATGACATGGTGGAGGTG′] 2452 NM_000016.5(ACADM): c.698T > 34 ACADM [′GAAGCAGATACCCCAGGAATTCAGAHTGGG C (p.Ile233Thr) AGAAAGGTAAAGTATTTATTA′] 2453 NM_004453.3(ETFDH): c.1852T > 2110 ETFDH [′AGGAGGACCTGCTTACAATGGAATGYAAAC C (p.Ter618Gln) TGCAGCTAGCCAGTTTCTTTC′] 2454 NM_000255.3(MUT): c.842T > C 4594 MUT [GCCATTCTGGAGCTGGCCTATACTTYAGCAG (p.Leu281Ser) ATGGATTGGAGTACTCTAGA′] 2455 NM_000277.1(PAH): c.2T > C 5053 PAH [′AGACCTCACTCCCGGGGAGCCAGCABGTCC (p.Met1Thr) ACTGCGGTCCTGGAAAACCCA′] 2456 NM_000017.3(ACADS): c.1057T > 35 ACADS [′GGCAGCCATGGCCAAGCTGGCCGCCYCGGA C (p.Ser353Pro) GGCCGCGACCGCCATCAGCCA′] 2457 NM_002225.3(IVD): c.295 + 2T > 3712 IVD [′GCGTATTGGGCATCACAGCCCCTGGYGAGT C ATAGTGTCTTTCCCTAAAAAG′] 2458 NM_000030.2(AGXT): c.2T > C 189 AGXT [′CGAGCGGCAGGTTGGGTGCGGACCAYGGCC (p.Met1Thr) TCTCACAAGCTGCTGGTGACC′] 2459 NM_000030.2(AGXT): c.77T > C 189 AGXT [′CTCTCCATCCCCAACCAGCTCCTGCYGGGGC (p.Leu26Pro) CTGGTCCTTCCAACCTGCCT′] 2460 NM_000030.2(AGXT): c.449T > C 189 AGXT [′GGCCTGGCCCAGCACAAGCCAGTGCYGCTG (p.Leu150Pro) TTCTTAACCCACGGGGAGTCG′] 2461 NM_000030.2(AGXT): c.497T > C 189 AGXT [′TCGTCCACCGGCGTGCTGCAGCCCCHTGATG (p.Leu166Pro) GCTTCGGGGAACTCTGCCAC′] 2462 NM_000030.2(AGXT): c.661T > C 189 AGXT [′CGCCCCTCCAGGGACCTCGCTCATCYCCTTC (p.Ser221Pro) AGTGACAAGGCCAAGTGAGT′] 2463 NM_000030.2(AGXT): c.757T > C 189 AGXT [′CAAGTGGCTGGCCAACTTCTGGGGCYGTGA (p.Cys253Arg) CGACCAGCCCAGGATGTGAGG′] 2464 NM_000030.2(AGXT): c.806T > C 189 AGXT [′CATCACACAATCCCCGTCATCAGCCYGTACA (p.Leu269Pro) GCCTGAGAGAGAGCCTGGCC′] 2465 NM_000030.2(AGXT): c.851T > C 189 AGXT [′CCAGCGCCATCTCCCACACAGGGCCYGGAG (p.Leu284Pro) AACAGCTGGCGCCAGCACCGC′] 2466 NM_000030.2(AGXT): c.893T > C 189 AGXT [′CAGCACCGCGAGGCCGCGGCGTATCYGCAT (p.Leu298Pro) GGGCGCCTGCAGGCACTGGGG′] 2467 NM_000030.2(AGXT): c.947T > C 189 AGXT [′TGAGCCAGGCCCCTCCTGCAGGCGCYCCGG (p.Leu316Pro) CTTCCCACAGTCACCACTGTG′] 2468 NM_000030.2(AGXT): c.1076T > 189 AGXT [′AGCCCGCCCTGTGCCCCCCAGGTGCYGCGG C (p.Leu359Pro) ATCGGCCTGCTGGGCTGCAAT′] 2469 NM_000030.2(AGXT): c.1151T > 189 AGXT [′GTGACGGAGGCCCTGAGGGCGGCCCYGCAG C (p.Leu384Pro) CACTGCCCCAAGAAGAAGCTG′] 2470 NM_012203.1(GRHPR): c.203T > 9380 GRHPR [′TCCGACCACGTGGACAAGAGGATCCYGGAT C (p.Leu68Pro) GCTGCAGGTGCACACTGGGTG′] 2471 NM_012203.1(GRHPR): c.965T > 9380 GRHPR [′CTGGCTGGCCTGAGAGGGGAGCCGABGCCT C (p.Met322Thr) AGTGAACTCAAGCTGTAGCCA′] 2472 NM_138413.3(HOGA1): c.533T > 112817 HOGA1 [′GTCCCAGCCAACACAGGGCTGGACCYGCCT C (p.Leu178Pro) GTGGATGCAGTGGTCACGCTT′] 2473 NM_138413.3(HOGA1): c.875T > 112817 HOGA1 [′GGGATCCCAGGGCTGAAGAAAATCAYGGAC C (p.Met292Thr) TGGTTTGGCTACTATGGAGGC′] 2474 NM_004370.5(COL12A1): c.7001T > 1303 COL12A1 [′TTCTTGACTGATGCCTCCTGGAGCAYTGGGG C (p.Ile2334Thr) ACGATAATTTTAACAAAGTT′] 2475 NM_019074.3(DLL4): c.583T > C 54567 DLL4 [′CCTGTGCAAGAAGCGCAATGACCACYTCGG (p.Phe195Leu) CCACTATGTGTGCCAGCCAGA′] 2476 NM_019074.3(DLL4): c.1168T > 54567 DLL4 [′CCAGGGGGCCAACTATGCTTGTGAAYGTCC C (p.Cys390Arg) CCCCAACTTCACCGGCTCCAA′] 2477 NM_015909.3(NBAS): c.3164T > 51594 NBAS [′TCAGAGCTTTTGGAAAAACATGGACYCGAG C (p.Leu1055Pro) AAACCAATTTCATTTGTTAAA′] 2478 NM_000263.3(NAGLU): c.1208T > 4669 NAGLU [′GCCTCCTTCCAGGGCCAGCCCTTCAYCTGGT C (p.Ile403Thr) GCATGCTGCACAACTTTGGG′] 2479 NM_203290.2(POLR1C): c.436T > 9533 POLR1C [′TCTACAGTTTCGTCTCCAGGTCAGAYGCACT C (p.Cys146Arg) CGGAACCCCCATGCTGCTAA′] 2480 NM_018359.3(UFSP2): c.868T > −1 — [CCAGGGCATATATGGCTATCATCATYATATG C (p.Tyr290His) CAGGATCGCATAGATGACAA′] 2481 NM_000310.3(PPT1): c.2T > C 5538 PPT1 [′CCGCGGTCATGTGACACAGCGAAGAYGGCG (p.Met1Thr) TCGCCCGGCTGCCTGTGGCTC′] 2482 NM_021007.2(SCN2A): c.1271T > 6326 SCN2A [′CTAATAAATTTGATCTTGGCTGTGGYGGCCA C (p.Val424Ala) TGGCCTATGAGGAACAGAAT′] 2483 NM_021007.2(SCN2A): c.2306T > 6326 SCN2A [′GTTGACCTGGCCATCACCATCTGCAYTGTCT C (p.Ile769Thr) TAAATACACTCTTCATGGCT′] 2484 NM_021007.2(SCN2A): c.4308 + 6326 SCN2A [′TATGCAGCTGTTGATTCACGAAATGYAAGTC 2T > C TAGTTAGAGGGAAATTGTTT′] 2485 NM_021007.2(SCN2A): c.4718T > 6326 SCN2A [′TGGATTAATCTGGTGTTTATTGTTCYGTTCA C (p.Leu1573Pro) CTGGAGAATGTGTGCTGAAA′] 2486 NM_001165963.1(SCN1A): c. −1 — [′CAGCTCATTGCCATGGATTTGCCCAHGGTGA 5567T > C (p.Met1856Thr) GTGGTGACCGGATCCACTGT′] 2487 NM_001165963.1(SCN1A): c. 6323 SCN1A [′AATCCCAATTATGGCTACACAAGCTYTGATA 1094T > C (p.Phe365Ser) CCTTCAGTTGGGCTTTTTTG′] 2488 NM_001165963.1(SCN1A): c. 6323 SCN1A [′TCGGCATTGAGAACATTCAGAGTTCYCCGA 662T > C (p.Leu221Pro) GCATTGAAGACGATTTCAGTC′] 2489 NM_000806.5(GABRA1): c.788T > 2554 GABRA1 [′ATTCAAACATACCTGCCATGCATAAYGACA C (p.Met263Thr) GTGATTCTCTCACAAGTCTCC′] 2490 NM_014191.3(SCN8A): c.4889T > 6334 SCN8A [′GCCCGTATTGGGCGCATCTTGCGTCYGATCA C (p.Leu1630Pro) AAGGCGCCAAAGGGATTCGT′] 2491 NM_005249.4(FOXG1): c.673T > 2290 FOXG1 [′TTACTACCGCGAGAACAAGCAGGGCYGGCA C (p.Trp225Arg) GAACTCCATCCGCCACAATCT′] 2492 NM_017882.2(CLN6): c.486 + 2T > 54982 CLN6 [′AAGAATCTCAAGCCGGAGACGCTGGYGAGG C CCACCTCCTGCTCCCTGCCTG′] 2493 NM_002693.2(POLG): c.1808T > 5428 POLG [′AGGGAAGCCATCCCAGGTAAGTGCCRTGAG C (p.Met603Thr) TTTAGGTGTGACCCGCATCTG′] 2494 NM_002693.2(POLG): c.1283T > 5428 POLG [′AGGCAGGTAGGAGACACCCATCTCCRGCAT C (p.Leu428Pro) GCCGGCCAGAGTCACTGGGTG′] 2495 NM_018129.3(PNPO): c.2T > C 55163 PNPO [′GGGTCACGTGGCCGGCGGCCCCCCAYGACG (p.Met1Thr) TGCTGGCTGCGGGGCGTCACG′] 2496 NM_007254.3(PNKP): c.1029 + 11284 PNKP [′ACCCCCACCCCCGCCCCAGGGCCTCRCCGGA 2T > C TCAAAGGCTGGGAGCTCGAA′] 2497 NM_172107.2(KCNQ2): c.583T > 3785 KCNQ2 [′CTCCCAGGGCAACGTCTTTGCCACAYCTGCG C (p.Ser195Pro) CTCCGGAGCCTGCGCTTCCT′] 2498 NM_000026.2(ADSL): c.340T > C 158 ADSL [′TATTCACCTTGGTGCTACTTCTTGCYATGTTG (p.Tyr114His) GAGACAATACTGTAGGCGC′] 2499 NM_000026.2(ADSL): c.1339T > 158 ADSL [CCAGTTGGATCATTTACTGGATCCTYCTTCT C (p.Ser447Pro) TTCACTGGTCGTGCCTCCCA′] 2500 NM_001356.4(DDX3X): c.704T > 1654 DDX3X [′GGGTCTGGAAAAACTGCAGCATTTCYGTTGC C (p.Leu235Pro) CCATCTTGAGTCAGATTTAT′] 2501 NM_001356.4(DDX3X): c.1175T > 1654 DDX3X [′TAAAAATTTTTTTTCTTTCAGATGCYGGCTC C (p.Leu392Pro) GTGATTTCTTAGATGAATAT′] 2502 NM_001356.4(DDX3X): c.1541T > 1654 DDX3X [′GACATTTCAAATGTGAAACATGTTAYCAATT C (p.Ile514Thr) TTGACTTGCCAAGTGATATT′] 2503 NM_020533.2(MCOLN1): c.317T > 57192 MCOLN1 [′ACCATCGCCTTCCGACACCTCTTCCYGCTGG C (p.Leu106Pro) GCTACTCGGACGGAGCGGAT′] 2504 NM_020533.2(MCOLN1): c.1340T > 57192 MCOLN1 [′TACTGCTTCTGTGGCTGGATCGTGCYGGGGC C (p.Leu447Pro) CCTATCATGTGAAGGTACAT′] 2505 NM_000083.2(CLCN1): c.1283T > 1180 CLCN1 [′CCCCGCGAAGCCATCAGTACTTTGTYTGACA C (p.Phe428Ser) ACAATACATGGGTGAAACAC′] 2506 NM_003995.3(NPR2): c.226T > C 4882 NPR2 [′CAGCTCCGAACTGGAAGGCGCCTGCYCTGA (p.Ser76Pro) GTACCTGGCACCGCTGAGCGC′] 2507 NM_004700.3(KCNQ4): c.808T > 9132 KCNQ4 [′GGACGCCAACTCCGACTTCTCCTCCYACGCC C (p.Tyr270His) GACTCGCTCTGGTGGGGGAC′] 2508 NM_004700.3(KCNQ4): c.823T > 9132 KCNQ4 [′CTTCTCCTCCTACGCCGACTCGCTCYGGTGG C (p.Trp275Arg) GGGACGGTGCGTGAGGGTCT′] 2509 NM_005097.3(LGI1): c.124T > C 9211 LGI1 [′GGGGAAGAAACCAGCGAAGCCAAAABGCCC (p.Cys42Arg) TGCCGTGTGTACTTGTACCAA′] 2510 NM_005572.3(LMNA): c.936 + 2T > 4000 LMNA [′CAGCTCAGCCAGCTCCAGAAGCAGGYGATA C CCCCACCTCACCCCTCTCTCC′] 2511 NM_006009.3(TUBA1A): c.1226T > 7846 TUBA [′AAACGTGCCTTTGTTCACTGGTACGYTGGGG C (p.Val409Ala) ′ AAGGGGATGGAGGAAGGTGAG′] 2512 NM_001081550.1(THOC2): c.1313T > 57187 THOC2 [′GACGTGTTCAATATGTTCTGTTACCYTGGTC C (p.Leu438Pro) CTCACCTTTCTCACGATCCC] 2513 NM_001081550.1(THOC2): c.3034T > 57187 THOC2 [′ACATCAACAGAAAACTCCAAATTTTYCCAC C (p.Ser1012Pro) ACTTCTTTGCTATGATCGAGT′] 2514 NM_001081550.1(THOC2): c.2399T > 57187 THOC2 [′GATTATATAAAGCGAGTGCCTTCAAYTGATG C (p.Ile800Thr) TACTCTGTAATGAATTTCAT′] 2515 NM_001356.4(DDX3X): c.1520T > 1654 DDX3X [′CAGGTAGCAGCAAGAGGACTGGACAYTTCA C (p.Ile507Thr) AATGTGAAACATGTTATCAAT′] 2516 NM_000158.3(GBE1): c.691 + 2T  > 2632 GBE1 [′TGAATAAAAATCACAGTTATTACTTRCCAAG C GCCTTTGATTCTTGGTAGTA′] 2517 NM_000719.6(CACNA1C): c.3497T > 775 CACNA1C [′ATGAACATCTTCGTGGGCTTCGTCAYCGTCA C (p.Ile1166Thr) CCTTTCAGGAGCAGGGGGAG′] 2518 NM_001105.4(ACVR1): c.587T > 90 ACVR1 [′TGTACATCAGGAAGTGGCTCTGGTCYTCCTT C (p.Leu196Pro) TTCTGGTACAAAGAACAGTG′] 2519 NM_024531.4(SLC52A2): c.935T > 79581 SLC52A2 [′CCCTACGGGCGTCTGGCCTACCACCYGGCTG C (p.Leu312Pro) TGGTGCTGGGCAGTGCTGCC′] 2520 NM_033409.3(SLC52A3): c.224T > 113278 SLC52A3 [′AGCTGCCTTTCCGAAGTGCCCATCAYCTTCA C (p.Ile75Thr) CCCTGCTGGGCGTGGGAACC′] 2521 NM_033409.3(SLC52A3): c.49T > 113278 SLC52A3 [′GGTCTGCGTCTTCGGAATGGGCTCCYGGGTG C (p.Trp17Arg) ACCATCAATGGGCTCTGGGT′] 2522 NM_133433.3(NIPBL): c.6108 + 25836 NIPBL [′CCATACCTTACCACTAAATGTAGTGYAAGTA 2T > C TAGAGCTGTCTTATTCTTGT′] 2523 NM_002397.4(MEF2C): c.2T > C 4208 MEF2C [′CGTAATCTGAATCTTTTTTCTCCCCDTAGTCC (p.Met1Thr) CCGTTTTTCTTCTCTCTCT′] 2524 NM_000124.3(ERCC6): c.2551T > 2074 ERCC6 [′AATACTCGCTGACCCTGCTTGTGCCRTATTT C (p.Trp851Arg) TCAACAAAGACTCAACAACA′] 2525 NM_000208.2(INSR): c.1610 + 2T > 3643 INSR [′GTTCATGCTGTTCTACAAAGAGGCGYAAGT C AGAAGAGTTAGAGAGACGCTG′] 2526 NM_000071.2(CBS): c.325T > C 875 CBS [′CTCCCGCCCGCGTTGAAGAACTCACRCTTGG (p.Cys109Arg) CCACTGGGAGGCAGAGATGA′] 2527 NM_004550.4(NDUFS2): c.875T > 4720 NDUFS2 [′CATTATGCTCTCCACAGTGGAGTGAYGCTTC C (p.Met292Thr) GGGGCTCAGGCATCCAGTGG′] 2528 NM_003119.3(SPG7): c.2228T > 6687 SPG7 [′AAGGAAGTGATAAACTATGAGGACAYTGAG C (p.Ile743Thr) GCTCTCATTGGCCCGCCGCCC′] 2529 NM_000546.5(TP53): c.584T > C 7157 TP53 [′CACACGCAAATTTCCTTCCACTCGGRTAAGA (p.Ile195Thr) TGCTGAGGAGGGGCCAGACC′] 2530 NM_018972.2(GDAP1): c.347T > 54332 GDAP1 [′AGGTTAATGCCTGATAAAGAAAGCAKGTAT C (p.Met116Thr) TACCCACGGGTACAACATTAC′] 2531 NM_000070.2(CAPN3): c.566T > 825 CAPN3 [′TGCCTGCCAACGTACAACAATCAACYGGTTT C (p.Leu189Pro) TCACCAAGTCCAACCACCGC′] 2532 NM_000070.2(CAPN3): c.1117T > 825 CAPN3 [′TTAATTCCTCCATTTTCCCACCAGAYGGAAG C (p.Trp373Arg) GACTGGAGCTTTGTGGACAA′] 2533 NM_181534.3(KRT25): c.950T > 147183 KRT25 [′AAATTGTTCTTTTCATACCGTGGCTRGGAGA C (p.Leu317Pro) GACTGAAGTTCAATTTCCAG′] 2534 NM_001002294.2(FMO3): c.560T > 2328 FMO3 [′CCAGGTGTATTCAATGGAAAGCGTGBCCTG C (p.Val187Ala) GTGGTTGGCCTGGGGAATTCG′] 2535 NM_001080522.2(CC2D2A): c. 57545 CC2D2A [′GAAATTCAAGCTGAAATAAGTGAACYGTTA 1676T > C (p.Leu559Pro) GAAGAGCACACGGAGGAGTAC′] 2536 NM_001080522.2(CC2D2A): c. 57545 CC2D2A [′TACCAAGTCATATTTTCTTCACAGAYTGATG 3596T > C (p.Ile1199Thr) GAACATTTAAAATAGATATT′] 2537 NM_025114.3(CEP290): c.2343T > 80184 CEP290 [′CCTGTAACAAATGTATTAAATATTCRTTCTG C (p.Asn781=) AGAATTAATGATACTGGCAC′] 2538 NM_030578.3(B9D2): c.107T > C 80776 B9D2 [′CGTTTGGCCCTCCCGCACGCCTGACRGGAGC (p.Leu36Pro) TTCCATGCCGCCCCTGCAGT′] 2539 NM_004304.4(ALK): c.3520T > C 238 ALK [′ACATCCCTCTCTGCTCTGCAGCAAAKTCAAC (p.Phe1174Leu) CACCAGAACATTGTTCGCTG′]

TABLE 7 Diseases/disorders containging A to G Changes.The table includes human gene mutations that may be corrected by changing a guanine (G) to adenine (A).The gene name, gene symbol, and Gene ID are indicated. SEQ ID Gene Gene NO: Name ID Symbol Flanks 3144 NM_017547.3(FOXRED1): c.1289A > 55572 FOXRED1 [′GTGGGCCCCCACCCGCTAGTTGTCAVCATG G (p.Asn430Ser) TACTTTGCTACTGGCTTCAGT′] 3145 NM_001006657.1(WDR35): c.25 − 57539 WDR35 [′CTAGTGCGGCTGTGGTTGCTTTTTCVGATT 2A > G TCCATTCCCAATAACGTGAAG′] 3146 NM_001006657.1(WDR35): c.1877A > 57539 WDR35 [′TATGTTTTCAGAAACTTGGATCCTGRGGTA G (p.Glu626Gly) AAAACAAGAAATGAGTGTTAA′] 3147 NM_000374.4(UROD): c.932A > G 7389 UROD [′GGCAACCTGGACCCCTGTGCCTTGTRTGCA (p.Tyr311Cys) TCTGAGGTAACAGCCAGGGCC′] 3148 NM_004315.4(ASAH1): c.155A > G 427 ASAH1 [′ACAGAGGACTGCAGAAAATCAACCTRTCC (p.Tyr52Cys) TCCTTCAGGACCAACGTGAGTA′] 3149 NM_004315.4(ASAH1): c.1006A > 427 ASAH1 [′GGGTAGATGGTATGTGGTACAAACARATT G (p.Asn336Asp) ATGACCGTTGGAAACATCCCTT′] 3150 NM_000071.2(CBS): c.1150A > G 875 CBS [′GGTGACTCCCCCATCCCGCAGGACCRAGTT (p.Lys384Glu) CCTGAGCGACAGGTGGATGCT′] 3151 NM_000274.3(OAT): c.734A > G 4942 OAT [′GGCGTTGTTGTTCCGGATCCAGGTTRCCTA (p.Tyr245Cys) ATGGGAGTGCGAGAGCTCTGC] 3152 NM_001385.2(DPYS): c.1001A > G 1807 DPYS [′GATAACTGCACTTTCAACACCTGCCRGAA (p.Gln334Arg) AGCTCTTGGGAAGGATGATTTT′] 3153 NN1_024598.3(USB1): c.502A > G 79650 USB1 [′GATTTACACCAATCAAGAGAAAACCRGGT (p.Arg168Gly) GGGTCCTCCCAACCCCCAATCA′] 3154 NM_001194958.2(KCNJ18): c.1097A > 100134444 KCNJ18 [′AGTGCGAAGGATCTGGTAGAGAACARGTT G (p.Lys366Arg) CCTGCTGCCCAGTGCCAACTCC′] 3155 NM_001914.3(CYB5A): c.130 − 1528 CYB5A [′TTTTTATTCTACCTCTCCTTTGAACRGCATC 2A > G CTGGTGGGGAAGAAGTTTTA′] 3156 NM_000398.6(CYB5R3): c.719A > 1727 CYB5R3 [′CGCTTCAAGCTCTGGTACACGCTGGRCAG G (p.Asp240Gly) AGCCCCTGAAGGTGAGTGAGGG′] 3157 NM_004628.4(XPC): c.413 − 24A > G 7508 XPC [′ATTATTATTGTTATTACTATTACTGRTTTTT AAAAATGCTTGTTGATAGAA′] 3158 NM_001134363.2(RBM20): c.1909A > 282996 RBM20 [′TGGCCCAGAAAGGCCGCGGTCTCGTRGTC  G (p.Ser637Gly) CGGTGAGCCGGTCACTCTCCCC′] 3159 NM_000552.3(VWF): c.2384A > G 7450 VWF [′GAGTGTACCAAAACGTGCCAGAACTRTGA (p.Tyr795Cys) CCTGGAGTGCATGAGCATGGGC′] 3160 NM_000552.3(VWF): c.1583A > G 7450 VWF [′ACCTGCGGCCTGTGTGGGAATTACARTGG (p.Asn528Ser) CAACCAGGGCGACGACTTCCTT′] 3161 NM_144573.3(NEXN): c.1955A > G 91624 NEXN [′ACTTTCCCAGAAGATGGAGGAGAGTRTAT (p.Tyr652Cys) GTGTAAAGCAGTCAACAATAAA′] 3162 NM_000267.3(NF1): c.4267A > G 4763 NF1 [′CGAAAGGGGCTTGAAGTTAATGTCARAGG (p.Lys1423Glu) TGAATTATTTTGATAATCTAGC′] 3163 NM_000267.3(NF1): c.1642 − 8A > G 4763 NF1 [′TCTCTTTGTCTTTCTCTTTTTTAAARAATTC AGGCTCTGCTGGTTCTTCAT′] 3164 NM_000267.3(NF1): c.1466A > G 4763 NF1 [′CCTACAGACCTGGAGACAAGAAGCTRTAA (p.Tyr489Cys) GTATCTTCTCTTGTCCATGGTG′] 3165 NM_000267.3(NF1): c.5944 − 5A > G 4763 NF1 [′AAAAACATGTTATTTTCCTTCTTCARCTAG ATTACAGATCTGCTTGATGTT′] 3166 NM_000308.2(CTSA): c.746 + 3A > G 5476 CTSA [′TACCATGGCCTTCTGGGGAACAGGTRTGG GATAGGGCAGTTGGGCAATCTC′] 3167 NM_000308.2(CTSA): c.200A > G 5476 CTSA [′CTGGCCAAGCAGCCGTCTTTCCGCCRGTAC (p.Gln67Arg) TCCGGCTACCTCAAAGGCTCC′] 3168 NM_000308.2(CTSA): c.1238A > G 5476 CTSA [′CTTTAGAAATACCAGATCCTATTATRTAAT (p.Tyr413Cys) GGAGATGTAGACATGGCCTGC′] 3169 NM_000308.2(CTSA): c.1411A > G 5476 CTSA [′CTCCCACATCGCCTTTCTCACGATCRAGGT (p.Lys471Glu) AGGGACTGGGCCTGCTGAGAG′] 3170 NM_000353.2(TAT): c.236 − 5A > G 6898 TAT [′TTCCAAACACAGTAGGGTCCCCTTTYTATG GGAGGAAAACACAAAAGGAGC′] 3171 NM_000097.5(CPOX): c.1210A > G 1371 CPOX [′TAATCTGCTGTATGATCGGGGCACARAGTT (p.Lys404Glu) TGGCCTCTTCACTCCAGGATC′] 3172 NM_000317.2(PTS): c.155A > G 5805 PTS [′AACAATCCAAATGGCCATGGGCACARTTA (p.Asn52Ser) TAAAGGTGAGAGAAAAACTGAT′] 3173 NM_000317.2(PTS): c.139A > G 5805 PTS [′ACTGTTTGGGAAATGCAACAATCCARATG (p.Asn47Asp) GCCATGGGCACAATTATAAAGG′] 3174 NM_000317.2(PTS): c.347A > G 5805 PTS [′GAAAATGTAGCTGTTTATATCTGGGRCAAC (p.Asp116Gly) CTCCAGAAAGTTCTTCCTGTA′] 3175 NM_000320.2(QDPR): c.449A > G 5860 QDPR [′CCCCATTTCCCAGGTATGATCGGGTRCGGC (p.Tyr150Cys) ATGGCCAAGGGTGCTGTTCAC′] 3176 NM_173560.3(RFX6): c.224 − 222546 RFX6 [′TCTATTTTTCTTTATCATCCCTTCARCTGGC 12A > G AATCAGAAATGCACTTAAAC′] 3177 NM_001999.3(FBN2): c.3725 − 2201 FBN2 [′TTCATCAATATCTGTGAAAACAGCAYTGC 15A > G AACCACATTGTCAGGTCTGCTT] 3178 NM_001040613.2(TMEM70): c.*7 − 54968 TMEM70 [′GATCCTCTCTCTTTTTTTCCCATTTRGGTGT 2A > G GAAATGTTTCTCTTATTCTA′] 3179 NM_152416.3(NDUFAF6): c.296A > 137682 NDUFAF6 [′AGGGCCTTTAATGTGGAACTGGCTCRGGCT G (p.Gln99Arg) GGTATTAAGATACCTTAAAAT′] 3180 NM_000140.3(FECH): c.1137 + 3A > 2235 FECH [′ACTAAAACGATTGTAACACTGTAGAYACC G TTAGAGAACAATGGATTTCCAT′] 3181 NM_000277.1(PAH): c.1A > G 5053 PAH [′GAGACCTCACTCCCGGGGAGCCAGCDTGT (p.Met1Val) CCACTGCGGTCCTGGAAAACCC′] 3182 NM_000277.1(PAH): c.611A > G 5053 PAH [′TCCTTGTATAAAACCCATGCTTGCTRTGAG (p.Tyr204Cys) TACAATCACATTTTTCCACTT] 3183 NM_000277.1(PAH): c.1241A > G 5053 PAH [′ATACCTCGGCCCTTCTCAGTTCGCTRCGAC (p.Tyr414Cys) CCATACACCCAAAGGATTGAG′] 3184 NM_000277.1(PAH): c.662A > G 5053 PAH [′CTTGAAAAGTACTGTGGCTTCCATGRAGAT (p.Glu221Gly) AACATTCCCCAGCTGGAAGAC] 3185 NM_000277.1(PAH): c.916A > G 5053 PAH [′TTCTATTTTCCCCCAATTACAGGAARTTGG (p.Ile306Val) CCTTGCCTCTCTGGGTGCACC′] 3186 NM_000277.1(PAH): c.1169A > G 5053 PAH [′TTCCAGCCCCTGTATTACGTGGCAGRGAGT (p.Glu390Gly) TTTAATGATGCCAAGGAGAAA′] 3187 NM_000277.1(PAH): c.1065 + 3A > G 5053 PAH [′TGTCATCCTTTGGTGAATTACAGGTVTGAC CTTCACAGGAACCAAGGATAG′] 3188 NM_000277.1(PAH): c.227A > G 5053 PAH [′AGACCTTCTCGTTTAAAGAAAGATGVGTA (p.Glu76Gly) TGAATTTTTCACCCATTTGGAT′] 3189 NM_000130.4(F5): c.1000A > G 2153 F5 [′CATTAAAAACTGCCCAAAGAAAACCRGGA (p.Arg334Gly) ATCTTAAGAAAATAACTCGTGA′] 3190 NM_000130.4(F5): c.5189A > G 2153 F5 [′GGCTCTGCCTGTCGGGCTTGGGCCTRCTAC (p.Tyr1730Cys) TCAGCTGTGAACCCAGTAGGT′] 3191 NM_000512.4(GALNS): c.1460A > 2588 GALNS [′TTGGTCCCCGCGCAGCCCCAGCTCARCGTG G (p.Asn487Ser) TGCAACTGGGCGGTCATGGTA′] 3192 NM_015702.2(MMADHC): c.746A 27249 MMADHC [′ACTCTTTTTGAAACTGATGAACGCTRCCGA > G (p.Tyr249Cys) CATTTAGGATTCTCTGTTGAT′] 3193 NM_015141.3(GPD1L): c.370A > G 23171 GPD1L [′TAACTTCTTGGCATCCTTGTAGGGCRTAGA (p.Ile124Val) CGAGGGCCCCGAGGGGCTGAA′] 3194 NM_013319.2(UBIAD1): c.305A > G 29914 UBIAD1 [′GTGCACGGGGCCGGTAATTTGGTCARCAC (p.Asn102Ser) TTACTATGACTTTTCCAAGGGC′] 3195 NM_013319.2(UBIAD1): c.355A > G 29914 UBIAD1 [′CATTGACCACAAAAAGAGTGATGACRGGA (p.Arg119Gly) CACTTGTGGACCGAATCTTGGA′] 3196 NM_013319.2(UBIAD1): c.695A > G 29914 UBIAD1 [′AGCACCGAGGCCATTCTCCATTCCARCAAC (p.Asn232Ser) ACCAGGGACATGGAGTCCGAC′] 3197 NM_013319.2(UBIAD1): c.335A > G 29914 UBIAD1 [′TACTATGACTTTTCCAAGGGCATTGRCCAC (p.Asp112Gly) AAAAAGAGTGATGACAGGACA] 3198 NM_000046.3(ARSB): c.629A > G 411 ARSB [′GTTGCAACAGGATATAAAAATATGTRTTC (p.Tyr210Cys) AACAAACATATTCACCAAAAGG] 3199 NM_006331.7(EMGl): c.257A > G 10436 EMG1 [′CGGGACCCTGGGGAAGCGCGGCCAGRTAT (p.Asp86Gly) CACCCACCAGGTAACTCCAGGG′] 3200 NM_000181.3(GUSB): c.1484A > G 2990 GUSB [′CTGCTGCTCTGGTCCTAGGCTCCGTRTGTG (p.Tyr495Cys) GATGTGATCTGTTTGAACAGC′] 3201 NM_000067.2(CA2): c.754A > G 760 CA2 [′CTGGCGCCCAGCTCAGCCACTGAAGRACA (p.Asn252Asp) GGCAAATCAAAGCTTCCTTCAA′] 3202 NM_000067.2(CA2): c.52A > G 760 CA2 [′TCCCCCAGGACCTGAGCACTGGCATVAGG (p.Lys18Glu) ACTTCCCCATTGCCAAGGGAGA′] 3203 NM_000404.2(GLB1): c.947A > G 2720 GLB1 [′TTTATAGGTGGGACCAATTTTGCCTRTTGG (p.Tyr316Cys) AATGGTAAGAGCACTTTAATA′] 3204 NM_000404.2(GLB1): c.1498A > G 2720 GLB1 [′TTTGCAGGGTTTGGTTTCTAACCTGRCTCT (p.Thr500Ala) CAGTTCCAATATCCTCACGGA′] 3205 NM_000404.2(GLB1): c.1480 − 2720 GLB1 [′GAGAGTCAGGTTAGAAACCAAACCCYGCA 2A > G AAGCAGAAACAGAGCACAGTGA′] 3206 NM_000404.2(GLB1): c.1772A > G 2720 GLB1 [′ATTAATGGCTTTAACCTTGGCCGCTRTTGG (p.Tyr591Cys) CCAGCCCGGGGCCCTCAGTTG′] 3207 NM_203447.3(DOCK8): c.1418A > 81704 DOCK8 [′ACTCTGAGCGTTAGCAGCTTTTTCARGCAG G (p.Lys473Arg) GTATCTCTTCACATTACAGTG′] 3208 NM_000275.2(OCA2): c.1465A > G 4948 OCA2 [′TGCCACTGCCATCGGGGACCCTCCARATGT (p.Asn489Asp) CATTATTGTTTCCAACCAAGA′] 3209 NM_152778.2(MFSD8): c.362A > G 256471 MFSD8 [′ATTTCCGTGGCAGCCAACTGCCTCTVTGCA (p.Tyr121Cys) TATCTCCACATCCCAGCTTCT′] 3210 NM_139241.3(FGD4): c.1762 − 121512 FGD4 [′ACTGTTCATTTTTCTTTTAAATTTARGGTGG 2A > G TTTGTTGGAAATGCTCCGAC′] 3211 NM_020223.3(FAM20C): c.1364 − 56975 FAM20C [′TGTGACACTTTCTGCCTCTCTCCGCRGGAA 2A > G ACATGGACCGTCACCACTACG′] 3212 NM_138387.3(G6PC3): c.346A > G 92579 G6PC3 [′GGCAGGCAGCCCTTCTGGACACTGCRTGA (p.Met116Val) TCACAGGAGCAGCCCTCTGGCC′] 3213 NM_018122.4(DARS2): c.133A > G 55157 DARS2 [′TTTTTTTTTTTTTTTTAAAGAATTCRGTAGC (p.Ser45Gly) TTTGTTGTCCGGACCAACAC′] 3214 NM_000642.2(AGL): c.4260 − 178 AGL [′CATCTTTTATTTAACTTAAATTTCARTCATT 12A > G TTGCAGTGATATGGTTTACT′] 3215 NM_000642.2(AGL): c.3439A > G 178 AGL [′TCTACTGGGTGAAGGAATTTATGCCRGATA (p.Arg1147Gly) CAATTGTCGGGATGCTGTGTG′] 3216 NM_145014.2(HYLS1): c.632A > G −1 — [′TTAAGCCGAAACCGGGGCAAGACAGRCCG (p.Asp211Gly) GGTAGCCCGGTATTTTGAGTAC′] 3217 NM_014362.3(HIBCH): c.365A > G 26275 HIBCH [′GCTCCAGTTTTCTTCAGAGAAGAATRTATG (p.Tyr122Cys) CTGAATAATGCTGTTGGTATG′] 3218 NM_014846.3(KIAA0196): c.1411A > 9897 KIAA0196 [′TTTTCTTATTTCTGTATTACTAGAARACCTT G (p.Asn471Asp) CAAGCTTGGTTCAGAGAGAT′] 3219 NM_000505.3(F12): c.158A > G 2161 F12 [′GAGCCCTGCCACTTCCCCTTCCAGTRCCAC (p.Tyr53Cys) CGGCAGCTGTACCACAAATGT′] 3220 NM_001029871.3(RSPO4): c.194A > 343637 RSPO4 [′TTCATCCGCCGGGAAGGCATCCGCCRGTA G (p.Gln65Arg) CGGCAAGTGCCTGCACGACTGT′] 3221 NM_017654.3(SAMD9): c.4483A > 54809 SAMD9 [′TAAAAGACTGGAAAGACTTGTTCACRAAG G (p.Lys1495Glu) GAAAAATTGACCAGTGCTTTAA′] 3222 NM_152419.2(HGSNAT): c.372 − 138050 HGSNAT [′TGTCTTAATTTTACCTAATGTTTGTRGGTTG 2A > G GAATACAGATTTGGAGAATT′] 3223 NM_001174089.1(SLC4A11): c.2518A > 83959 SLC4A11 [′CATGAGCTCCCTGCCCTACATGAAGRTGAT G (p.Met840Val) CTTTCCCCTCATCATGATCGC′] 3224 NM_012073.4(CCT5): c.440A > G 22948 CCT5 [′CAGGCTGCTCGTGTTGCTATTGAACRCCTG (p.His147Arg) GACAAGATCAGCGATAGCGTC′] 3225 NM_025114.3(CEP290): c.2991 + 80184 CEP290 [′CACCTGGCCCCAGTTGTAATTGTGARTATC 1655A > G TCATACCTATCCCTATTGGCA′] 3226 NM_153704.5(TMEM67): c.870 − 91147 TMEM67 [′TATTAAAACAGTTGTAACTGTTTATRGGAG 2A > G ACAGAATCTTCCTTGGCTGTT′] 3227 NM_153704.5(TMEM67): c.1538A > 91147 TMEM67 [′TATCAGGTTTCTTTCTCAGTCACATDTGAA G (p.Tyr513Cys) ATGGATCATGGAGAAGCACAT′] 3228 NM_001041.3(SI): c.350A > G 6476 SI [′GTTGATAATCATGGTTATAACGTTCRAGAC (p.Gln117Arg) ATGACAACAACAAGTATTGGT′] 3229 NM_015697.7(COQ2): c.890A > G 27235 COQ2 [′TCTGGAGTTATGTGGACACTAATATRTGAT (p.Tyr297Cys) ACTATTTATGCCCATCAGGTA′] 3230 NM_015697.7(COQ2): c.683A > G 27235 COQ2 [′GCACTGGGTGTTCTTCTGTGTCTAARTTAC (p.Asn228Ser) TACAGGTATATTAAACGTTTT′] 3231 NM_000190.3(HMBS): c.1A > G 3145 HMBS [′CACAGCCTACTTTCCAAGCGGAGCCRTGTC (p.Met1Val) TGGTAACGGCAATGCGGCTGC′] 3232 NM_004035.6(ACOX1): c.832A > G 51 ACOX1 [′GAGTAACAAGCTGACTTACGGGACCRTGG (p.Met278Val) TGTTTGTCAGGTCCTTCCTTGT′] 3233 NM_004035.6(ACOX1): c.926A > G 51 ACOX1 [′ATCCGATACAGCGCTGTGAGGCACCRGTC (p.Gln309Arg) TGAAATCAAGCCAGGGTAAGGA′] 3234 NM_000237.2(LPL): c.548A > G 4023 LPL [′TGCTTTTTTCCCTTTTAAGGCCTCGRTCCAG (p.Asp183Gly) CTGGACCTAACTTTGAGTAT′] 3235 NM_000237.2(LPL): c.953A > G 4023 LPL [′TGCAACAATCTGGGCTATGAGATCARTAA (p.Asn318Ser) AGTCAGAGCCAAAAGAAGCAGC′] 3236 NM_001171993.1(HPD): c.362A > G 3242 HPD [′TACATCGGCCAATTCTTGCCTGGATRTGAG (p.Tyr121Cys) GCCCCAGCGTTCATGGACCCC′] 3237 NM_139281.2(WDR36): c.1064A > 134430 WDR36 [′CTTCTTGTCACAAATGGCGCTGACARTGCT G (p.Asn355Ser) CTTAGGGTATTATGATTATTG′] 3238 NM_001127255.1(NLRP7): c.2738A > −1 — [′CTCACAAACCTGGACTTGAGTATCARCCA G (p.Asn913Ser) GATAGCTCGTGGATTGTGGATT′] 3239 NM_016417.2(GLRX5): c.294A > G 51218 GLRX5 [′TGCTGGACGACCCGGAGCTCCGACARGGT (p.Gln98=) CAGGCCAGTGTGCCGGGCAGGC′] 3240 NM_024884.2(L2HGDH): c.293A > 79944 L2HGDH [′ACTGGACATAACAGTGGTGTCATACRTAG G (p.His98Arg) TGGAATTTATTATAAACCTGAG′] 3241 NM_000382.2(ALDH3A2): c.1157A > 224 ALDH3A2 [′ACATCCAGTGGAGGTGTCACAGGCARTGA G (p.Asn3865er) CGTCATTATGCACTTCACGCTC′] 3242 NM_018105.2(THAP1): c.266A > G 55145 THAP1 [′TTTCTTTGTACTGAGCCACATGACARGGTA (p.Lys89Arg) ATATGCATTTTAAAATATTGG′] 3243 NM_000785.3(CYP27B1): c.566A > 1594 CYP27B1 [′GCCCTGGTTCGGGACGTGGCGGGGGRATT G (p.Glu189Gly) TTACAAGTTCGGACTGGAAGGT′] 3244 NM_182548.3(LHFPL5): c.380A > G 222662 LHFPL5 [′TTCATCTGCAACACGGCCACAGTCTRTAAG (p.Tyr127Cys) ATCTGTGCATGGATGCAGCTG′] 3245 NM_015040.3(PIKFYVE): c.3308A > 200576 PIKFYVE [′TTCAAAGAAATGGAGAACAGGAGGARGA G (p.Lys1103Arg) AACAGCTGCTCAGGGATCTCTCT′] 3246 NM_138701.3(MPLKIP): c.430A > G 136647 MPLKIP [′GTTGGAAAATTATTTCAAGCCTTCARTGCT (p.Met144Val) TGAAGATCCTTGGGCTGGCCT′] 3247 NM_152783.4(D2HGDH): c.1315A > 728294 D2HGDH [′TGCCCTTGTCCCTCCAGGAGATGGTRACCT G (p.Asn439Asp) GCACCTCAATGTGACGGCGGA′] 3248 NM_022912.2(REEP1): c.183 − 65055 REEP1 [′AACTTGTGTTTTCATCTCTCTCTCCRGGTTT 2A > G CCATTCTATTATGAACTAAA′] 3249 NM_000060.3(BTD): c.755A > G 686 BTD [′ACATGCTTTGATATATTGTTCTTTGRCCCT (p.Asp252Gly) GCCATCAGAGTCCTCAGAGAC′] 3250 NM_022132.4(MCCC2): c.1309A > 64087 MCCC2 [′TGTGGCCTGTGCCCAAGTGCCTAAGDTAA G (p.Ile437Val) CCCTCATCATTGGGGGCTCCTA′] 3251 NM_022132.4(MCCC2): c.569A > G 64087 MCCC2 [′GCAGATGTGTTTCCAGATCGAGACCRCTTT (p.His190Arg) GGCCGTACATTCTATAATCAG′] 3252 NM_198578.3(LRRK2): c.5096A > G 120892 LRRK2 [′ATCATCCGACTATATGAAATGCCTTRTTTT (p.Tyr1699Cys) CCAATGGGATTTTGGTCAAGA′] 3253 NM_198578.3(LRRK2): c.3364A > G 120892 LRRK2 [′TGTGACTAGAAATAAAATATCAGGGRTAT (p.Ile1122Val) GCTCCCCCTTGAGACTGAAGGA′] 3254 NM_000022.2(ADA): c.219 − 2A > G 100 ADA [′TTCCCAACCCCTTTCTTCCCTTCCCRGGGG CTGCCGGGAGGCTATCAAAAG′] 3255 NM_000022.2(ADA): c.290A > G 100 ADA [′ATGAAGGCCAAAGAGGGCGTGGTGTDTGT (p.Tyr97Cys) GGAGGTGCGGTACAGTCCGCAC′] 3256 NM_017780.3(CHD7): c.3082A > G 55636 CHD7 [′TTTAGTAATTGCCCCATTGTCCACARTCCC (p.Ile1028Val) CAACTGGGAAAGGGAATTCCG′] 3257 NM_017780.3(CHD7): c.164A > G 55636 CHD7 [′TTTGCCTCTTTACAGCCATCCCTTCRTCATC (p.His55Arg) CTTCAACTAATCAAAATCAA′] 3258 NM_152443.2(RDH12): c.677A > G 145226 RDH12 [′GTCCCAGGCACCGGGGTCACCACCTRCGC (p.Tyr226Cys) AGTGCACCCAGGCGTCGTCCGC′] 3259 NM_015335.4(MED13L): c.752A > 23389 MED13L [′TACCCGATGGTGCTAAAAAAGAAAGRAGA G (p.Glu251Gly) ATCGAAAGAGGAAGACGAGTTG′] 3260 NM_015335.4(MED13L): c.6068A > 23389 MED13L [′GCTTTTTTTCCCCCCTTCCCTCTAGRTGATA G (p.Asp2023Gly) TGTTTGTTGACCTTCCATTC′] 3261 NM_025265.3(TSEN2): c.926A > G 80746 TSEN2 [′TATTTTCAGGCCTTTTTCTTGGTCTRTGCTC (p.Tyr309Cys) TGGGATGTTTAAGTATTTAC′] 3262 NM_015384.4(NIPBL): c.7289A > G 25836 NIPBL [′AAAACAGACGTGACTATGCTCTTGTRTATA (p.Tyr2430Cys) GCAGACAATCTAGCCTGTTTT′] 3263 NM_207352.3(CYP4V2): c.1091 − 285440 CYP4V2 [′GTCATCTTATCTACTTGCTTTCATCRGGGA 2A > G AGTCTGACCGTCCCGCTACAG′] 3264 NM_024006.5(VKORC1): c.172A > 79001 VKORC1 [′CAGCTGTTCGCGCGTCTTCTCCTCCRGGTG G (p.Arg58Gly) TGCACGGGAGTGGGAGGCGTG′] 3265 NM_000551.3(VHL): c.491A > G 7428 VHL [′TATACTCTGAAAGAGCGATGCCTCCRGGTT (p.Gln164Arg) GTCCGGAGCCTAGTCAAGCCT] 3266 NM_000101.3(CYBA): c.281A > G 1535 CYBA [′AATTACTATGTTCGGGCCGTCCTGCRTCTC (p.His94Arg) CTGTGAGTCCCCGTCCCGCAC′] 3267 NM_014874.3(MFN2): c.827A > G 9927 MFN2 [′TTGTTTGGGCTCCAGGTGCGGCGGCRGCAC (p.Gln276Arg) ATGGAGCGTTGTACCAGCTTC′] 3268 NM_015046.5(SETX): c.1807A > G 23064 SETX [′GCAGAAGTCAGATCCACAAAAGTGTYACA (p.Asn603Asp) TGGAGGTGCTTTGAATTTTATG′] 3269 NM_005609.2(PYGM): c.1A > G 5837 PYGM [′CAGTCCGGCCCGCCCTCCTGCAGCCVTGTC (p.Met1Val) CCGGCCCCTGTCAGACCAAGA′] 3270 NM_033071.3(SYNE1): c.15705 − 23345 SYNE1 [′ACTTCACTTCTGTTTTATCCCTGTARTGTTT 12A > G CTGAAGCATGCAGGAGAAAG′] 3271 NM_130468.3(CHST14): c.878A > G 113189 CHST14 [′CTGTGCCAGCCTTGTGCCGTGCACTRTGAC (p.Tyr293Cys) TTTGTGGGCTCCTATGAGAGG′] 3272 NM_206933.2(USH2A): c.14020A > 7399 USH2A [′AAAAGTTTTGTATTACGAATTATACRGAAG G (p.Arg4674Gly) ACAAATAGCAACTCAGCCTAG′] 3273 NM_000048.3(ASL): c.857A > G 435 ASL [′AGCACGGGAAGCAGCCTGATGCCCCRGAA (p.Gln286Arg) GAAAAACCCCGACAGTTTGGAG′] 3274 NM_001875.4(CPS1): c.1010A > G 1373 CPS1 [′CAGGCTTTCATTACTGCTCAGAATCRTGGC (p.His337Arg) TATGCCTTGGACAACACCCTC′] 3275 NM_001173464.1(KIF21A): c.2839A > 55605 KIF21A [′GACCATTTCCAACATGGAGGCAGATRTGA G (p.Met947Val) ATAGACTCCTCAAGGTGTGGAA] 3276 NM_000026.2(ADSL): c.736A > G 158 ADSL [′CATCACAGGGCAGACATATACACGARAAG (p.Lys246Glu) TGGATATTGAAGTACTGTCTGT′] 3277 NM_024577.3(SH3TC2): c.530 − 79628 SH3TC2 [′AGCCATTGCTTTTCTCATTATCCACRGGCC 2A > G ACTTCTTCTGCAGAGCCCTGT′] 3278 NM_018249.5(CDK5RAP2): c.4005 − 55755 CDK5RAP2 [′AGCATGGGAGATGATTTATCTTTCARTCTC 15A > G AATGGTTAAGGATTGAGGAAG′] 3279 NM_000346.3(SOX9): c.517A > G 6662 SOX9 [′GCACAAGAAGGACCACCCGGATTACRAGT (p.Lys173Glu) ACCAGCCGCGGCGGAGGAAGTC′] 3280 NM_144596.3(TTC8): c.115 − 2A > G 123016 TTC8 [′GTTTACTGCCTTCTTAATGCTTTCCRGGAA CCAGATCCTGAATTGCCAGTG′] 3281 NM_000243.2(MEFV): c.2080A > G 4210 MEFV [′GAATGGCTACTGGGTGGTGATAATGDTGA (p.Met694Val) AGGAAAATGAGTACCAGGCGTC′] 3282 NM_000243.2(MEFV): c.2084A > G 4210 MEFV [′GGCTACTGGGTGGTGATAATGATGADGGA (p.Lys695Arg) AAATGAGTACCAGGCGTCCAGC′] 3283 NM_006493.2(CLN5): c.1121A > G 1203 CLN5 [′GTGCACAAACAGTTCTATTTGTTTTRTAAT (p.Tyr374Cys) TTTGAATATTGGTTTTTACCT′] 3284 NM_000483.4(APOC2): c.1A > G −1 — [′TCAATGTTCCAGGTCTCTGGACACTRTGGG (p.Met1Val) CACACGACTCCTCCCAGCTCT′] 3285 NM_058172.5(ANTXR2): c.1142A > 118429 ANTXR2 [′TGGCCAACTGTGGATGCTTCCTATTDTGGT G (p.Tyr381Cys) GGTCGAGGGGTTGGAGGAATT′] 3286 NM_001128085.1(ASPA): c.692A > −1 — [′TATAAAATTATAGAGAAAGTTGATTRCCCC G (p.Tyr231Cys) CGGGATGAAAATGGAGAAATT′] 3287 NM_001128085.1(ASPA): c.71A > G −1 — [′ATCTTTGGAGGAACCCATGGGAATGRGCT (p.Glu24Gly) AACCGGAGTATTTCTGGTTAAG′] 3288 NM_014365.2(HSPB8): c.421A > G 26353 HSPB8 [′TGGCATTGTTTCTAAGAACTTCACARAGAA (p.Lys141Glu) AATCCAGTAAGTAACCTGGAG′] 3289 NM_000391.3(TPP1): c.857A > G 1200 TPP1 [′CAGTACCTGATGAGTGCTGGTGCCARCATC (p.Asn286Ser) TCCACCTGGGTCTACAGTAGC′] 3290 NM_000391.3(TPP1): c.887 − 10A > G 1200 TPP1 [′TGTCCCTCATGCCGGCCTGGATTTTYTTTTT TTTTTTTTTTGAGGGATGGG′] 3291 NM_182760.3(SUMF1): c.1A > G 285362 SUMF1 [′CGGGTCACATGGCCCGCGGGACAACRTGG (p.Met1Val) CTGCGCCCGCACTAGGGCTGGT′] 3292 NM_031443.3(CCM2): c.1A > G 83605 CCM2 [′GGCCGCGGGAGCCGCACGCGGCGATRTGG (p.Met1Val) AAGAGGAGGGCAAGAAGGGCAA′] 3293 NM_004183.3(BEST1): c.707A > G 7439 BEST1 [′GACTGGATTAGTATCCCACTGGTGTRTACA (p.Tyr236Cys) CAGGTGAGGACTAGGCTGGTG′] 3294 NM_004183.3(BEST1): c.680A > G 7439 BEST1 [′ACTCAGTGTGGACACCTGTATGCCTRCGAC (p.Tyr227Cys) TGGATTAGTATCCCACTGGTG′] 3295 NM_000158.3(GBE1): c.1634A > G 2632 GBE1 [′TGTAATGCAGGTAATGAATTTGGGCRTCCT (p.His545Arg) GAATGGTTAGACTTCCCAAGA′] 3296 NM_000158.3(GBE1): c.1883A > G 2632 GBE1 [′GGTCTTCTTTTCATTTTCAACTTCCRTCCAA (p.His628Arg) GCAAGAGCTACACTGACTAC′] 3297 NM_017890.4(VPS13B): c.8978A > 157680 VPS13B [′CTTCTGCCCTGGGCCCTGCTTATCARTGAA G (p.Asn2993Ser) TCCAAATGGGACCTCTGGCTA′] 3298 NM_000019.3(ACAT1): c.278A > G 38 ACAT1 [′GAAGTGAAAGAAGCATACATGGGTARTGT (p.Asn93Ser) TCTACAAGGAGGTGAAGGACAA′] 3299 NM_173076.2(ABCA12): c.4139A > 26154 ABCA12 [′CATATTACTTCATTGCTGGGGCCCARTGGA G (p.Asn1380Ser) GCTGGGAAAACTACTACCATG′] 3300 NM_030665.3(RAH): c.4685A > G 10743 RAH [′AAGGGGCGTGCCAAGCGACGACGACRGCA (p.Gln1562Arg) GCAGGTGCTGCCCCTGGATCCC] 3301 NM_000271.4(NPC1): c.3467A > G 4864 NPC1 [′AGTCTGAACGCTGTATCCTTGGTCARCCTG (p.Asn1156Ser) GTGATGGTGAGTCCTCATACA′] 3302 NM_000271.4(NPC1): c.3263A > G 4864 NPC1 [′CCCTCCAGTGTGTTTTATGTCTTCTRCGAA (p.Tyr1088Cys) CAGTACCTGACCATCATTGAC′] 3303 NM_000543.4(SMPD1): c.1154A > 6609 SMPD1 [′CTCCGCCTCATCTCTCTCAATATGARTTTTT G (p.Asn385Ser) GTTCCCGTGAGAACTTCTGG′] 3304 NM_000226.3(KRT9): c.469A > G 3857 KRT9 [′TCTGACTGCTAATGAGAAGAGCACCRTGC (p.Met157Val) AGGAACTCAATTCTCGGCTGGC′] 3305 NM_000226.3(KRT9): c.482A > G 3857 KRT9 [′GAGAAGAGCACCATGCAGGAACTCADTTC (p.Asn161Ser) TCGGCTGGCCTCTTACTTGGAT] 3306 NM_176824.2(BBS7): c.968A > G 55212 BBS7 [′ACAGGTCTGACAACAGAGCCCATTCRTAA (p.His323Arg) GGAAAGTGGACCAGGAGAAGAA′] 3307 NM_000051.3(ATM): c.3118A > G 472 ATM [′GAGGAAATATATATTCTCTGTAAGARTGG (p.Met1040Val) CCCTAGTAAATTGCCTTAAAAC′] 3308 NM_000051.3(ATM): c.7268A > G 472 ATM [′GCTCTCCTGAAAAGAGCCAAAGAGGRAGT (p.Glu2423Gly) AGGTCTCCTTAGGGAACATAAA′] 3309 NM_000051.3(ATM): c.8030A > G −1 — [′TATTAGGTGGACCACACAGGAGAATRTGG (p.Tyr2677Cys) AAATCTGGTGACTATACAGTCA] 3310 NM_000487.5(ARSA): c.*96A > G 410 ARSA [′GAGGGGGTTTGTGCCTGATAACGTARTAA CACCAGTGGAGACTTGCAGATG′] 3311 NM_000487.5(ARSA): c.1055A > G 410 ARSA [′GCCCTGGCTGGGGCCCCACTGCCCARTGTC (p.Asn352Ser) ACCTTGGATGGCTTTGACCTC′] 3312 NM_000218.2(KCNQ1): c.418A > G 3784 KCNQ1 [′CATCGTCCTGGTCTGCCTCATCTTCRGCGT (p.Ser140Gly) GCTGTCCACCATCGAGCAGTA′] 3313 NM_172250.2(MMAA): c.620A > G 166785 MMAA [′GAGTTATCAAGAGATATGAATGCATRCAT (p.Tyr207Cys) CAGGCCATCTCCTACTAGAGGA′] 3314 NM_000187.3(HGD): c.1112A >   3081 HGD [′AGTCTACACAGCACAATGACCCCCCRTGG (p.His371Arg) ACCTGATGCTGACTGCTTTGAG′] 3315 NM_000187.3(HGD): c.1102A > G 3081 HGD [′AGGGGGAGGGAGTCTACACAGCACARTGA (p.Met368Val) CCCCCCATGGACCTGATGCTGA′] 3316 NM_138477.2(CDAN1): c.1796A > 146059 CDAN1 [′CTGAGCTTGAAGATCCAGGAGCTCARTGG G (p.Asn599Ser) TCTTGCCCTGCCCCAGCATGAG′] 3317 NM_001079802.1(FKTN): c.1112A > 2218 FKTN [′GTAAAACTTGATGTTTTTTTCTTCTRTGAA G (p.Tyr371Cys) GAAACTGATCACATGTGGAAT′] 3318 NM_013382.5(POMT2): c.1997A > 29954 POMT2 [′GCTTGAGAAGAGCATGGCTGGGAAGYAGT G (p.Tyr666Cys) GGTGGAAGTAGAGGACCCGGCC′] 3319 NM_000529.2(MC2R): c.761A > G 4158 MC2R [′CCAAGTAACCCCTACTGCGCCTGCTRCATG (p.Tyr254Cys) TCTCTCTTCCAGGTGAACGGC] 3320 NM_000383.3(AIRE): c.247A > G 326 AIRE [′CCTGGACTTCTGGAGGGTGCTGTTCRAGGA (p.Lys83Glu) CTACAACCTGGAGCGCTATGG′] 3321 NM_020436.3(SALL4): c.2663A > G 57167 SALL4 [′TCGTCTGCTAGCGCTCTTCAGATCCRCGAG (p.His888Arg) CGGACTCACACTGGAGAGAAG′] 3322 NM_203288.1(RP9): c.509A > G 6100 RP9 [′CAGTTACTGGAGGATTCTACCTCAGRTGAA (p.Asp170Gly) GATAGGAGCAGCTCCAGTTCC′] 3323 NM_000348.3(SRD5A2): c.692A > G 6716 SRD5A2 [′TTCCTTGGGCTGCGAGCTTTTCACCRCCAT (p.His231Arg) AGGTAAATTTTTCAATAAAAG′] 3324 NM_006445.3(PRPF8): c.6926A > G 10594 PRPF8 [′CCCAAAGAGTTCTACCACGAGGTGCVCAG (p.His2309Arg) GCCCTCTCACTTCCTCAACTTT′] 3325 NM_018319.3(TDP1): c.1478A > G 55775 TDP1 [′TCTGGCCGCAGCAATGCCATGCCACRTATT (p.His493Arg) AAGACATATATGAGGCCTTCT′] 3326 NM_144773.2(PROKR2): c.629A > 128674 PROKR2 [′AGCCAGGAGAAGATCTTCTGTGGCCRGAT G (p.Gln210Arg) CTGGCCTGTGGATCAGCAGCTC′] 3327 NM_001604.5(PAX6): c.1075 − 5080 PAX6 [′ACCACAGGTTTGCCTCTCTCCTCACRGCCC 2A > G CCAGTCCCCAGCCAGACCTCC′] 3328 NM_000280.4(PAX6): c.1171A > G 5080 PAX6 [′CAGTCAGCCAATGGGCACCTCGGGCRCCA (p.Thr391Ala) CTTCAACAGGTGAGCCACTGCT′] 3329 NM_024426.4(WT1): c.1391A > G 7490 WT1 [′TGTCAGCGAAAGTTCTCCCGGTCCGRCCAC (p.Asp464Gly) CTGAAGACCCACACCAGGACT′] 3330 NM_024426.4(WT1): c.1021A > G 7490 WT1 [′TTGCAGCCACAGCACAGGGTACGAGRGCG (p.Ser341Gly) ATAACCACACAACGCCCATCCT′] 3331 NM_005957.4(MTHFR): c.971A > G 4524 MTHFR [′CCAGGCCTCCACTTCTACACCCTCARCCGC (p.Asn324Ser) GAGATGGCTACCACAGAGGTG′] 3332 NM_001127328.2(ACADM): c.997A > 34 ACADM [′TAACTCATTCTAGCTAGTTCAACTTYCATT G (p.Lys333Glu) GCCATTTCAGCCAGCATAAAT′] 3333 NM_001127328.2(ACADM): c.589A > 34 ACADM [′TATTAATGGTCAGAAGATGTGGATARCCA G (p.Thr197Ala) ACGGAGGAAAAGCTAATTGGTA′] 3334 NM_000155.3(GALT): c.940A > G 2592 GALT [′AGGATCAGAGGCTGGGGCCAACTGGRACC (p.Asn314Asp) ATTGGCAGCTGCACGCTCATTA′] 3335 NM_000155.3(GALT): c.563A > G 2592 GALT [′TGTTCTAACCCCCACCCCCACTGCCRGGTA (p.Gln188Arg) AGGGTGTCAGGGGCTCCAGTG′] 3336 NM_000250.1(MPO): c.518A > G 4353 MPO [′GTTGCACATCCCGGTGATGGTGCGGYATTT (p.Tyr173Cys) GTCCTGCTCCGGGCAAGTCAC′] 3337 NM_020247.4(ADCK3): c.1541A > 56997 ADCK3 [′TTGGATTTTGGGGCAACGCGGGAATRTGA G (p.Tyr514Cys) CAGATCCTTCACCGACCTCTAC′] 3338 NM_000229.1(LCAT): c.463A > G 3931 LCAT [′CACACTGGTGCAGAACCTGGTCAACRATG (p.Asn155Asp) GCTACGTGCGGGACGAGACTGT′] 3339 NM_000403.3(GALE): c.101A > G 2582 GALE [′GGCTACTTGCCTGTGGTCATCGATARCTTC (p.Asn34Ser) CATAATGCCTTCCGTGGTGAG′] 3340 NM_001008216.1(GALE): c.308A > 2582 GALE [′GGCGAGTCGGTGCAGAAGCCTCTGGRTTA G (p.Asp103Gly) TTACAGAGTTAACCTGACCGGG′] 3341 NM_000403.3(GALE): c.770A > G 2582 GALE [′AAGGGCCACATTGCAGCCTTAAGGARGCT (p.Lys257Arg) GAAAGAACAGTGTGGCTGCCGG′] 3342 NM_000527.4(LDLR): c.2483A > G 3949 LDLR [′AGCATCAACTTTGACAACCCCGTCTRTCAG (p.Tyr828Cys) AAGACCACAGAGGATGAGGTC′] 3343 NM_024740.2(ALG9): c.860A > G 79796 ALG9 [′ATTGCACCACTCAACATTGTTTTGTRTAAT (p.Tyr287Cys) GTCTTTACTCCTCATGGACCT′] 3344 NM_000375.2(UROS): c.184A > G 7390 UROS [′TGAAGATTACGGGGGACTCATTTTTRCCAG (p.Thr62Ala) CCCCAGAGCAGTGGAAGCAGC′] 3345 NM_000372.4(TYR): c.125A > G 7299 TYR [′GAATGCTGTCCACCGTGGAGCGGGGRCAG (p.Asp42Gly) GAGTCCCTGTGGCCAGCTTTCA′] 3346 NM_000372.4(TYR): c.1A > G 7299 TYR [′AGACCTTGTGAGGACTAGAGGAAGARTGC (p.Met1Val) TCCTGGCTGTTTTGTACTGCCT′] 3347 NM_000053.3(ATP7B): c.3809A > G 540 ATP7B [′GTCGCCATGGTGGGGGATGGGGTCADTGA (p.Asn1270Ser) CTCCCCGGCCTTGGCCCAGGCA′] 3348 NM_000520.4(HEXA): c.1A > G 3073 HEXA [′CTCCGAGAGGGGAGACCAGCGGGCCVTGA (p.Met1Val) CAAGCTCCAGGCTTTGGTTTTC′] 3349 NM_000520.4(HEXA): c.611A > G 3073 HEXA [′AATAAATTGAACGTGTTCCACTGGCRTCTG (p.His204Arg) GTAGATGATCCTTCCTTCCCA′] 3350 NM_000356.3(TCOF1): c.149A > G 6949 TCOF1 [′CAGCCCGTAACCCTTCTGGACATCTRTACA (p.Tyr50Cys) CACTGGCAACAGTAAGTGGTG′] 3351 NM_000144.4(FXN): c.385 − 2A > G 2395 FXN [′ATGCTTTTTTTCCACCTAATCCCCTRGAGT GGTGTCTTAACTGTCAAACTG′] 3352 NM_016335.4(PRODH): c.1562A > 5625 PRODH [′GCCTAGCAGCTGTCCAAAGTACACCYGGT G (p.Gln521Arg) GGTCAGCAGGATGCAGGCCCAG′] 3353 NM_015474.3(SAMHD1): c.760A > 25939 SAMHD1 [′TAATTCTAATGGAATTAAGCCTGTCRTGGA G (p.Met254Val) ACAATATGGTCTCATCCCTGA′] 3354 NM_012464.4(TLL1): c.1885A > G 7092 TLL1 [′ACTTCTTACCAAACTTAACGGCACCRTAAC (p.Ile629Val) CACCCCTGGCTGGCCCAAGGA′] 3355 NM_000112.3(SLC26A2): c.1273A > 1836 SLC26A2 [′GGAAATGTATGCCATTGGCTTTTGTRATAT G (p.Asn425Asp) CATCCCTTCCTTCTTCCACTG′] 3356 NM_138691.2(TMC1): c.1960A > G 117531 TMC1 [′CCTGTCCACAATGCCTGTCTTGTACRTGAT (p.Met654Val) CGTGTCCCTCCCACCATCTTT′] 3357 NM_000173.6(GP1BA): c.763A > G 2811 GP1BA [′GAAGCAAGGTGTGGACGTCAAGGCCRTGA (p.Met255Val) CCTCTAATGTGGCCAGTGTGCA′] 3358 NM_024996.5(GFM1): c.521A > G 85476 GFM1 [′AACGTTCCGTTTCTAACTTTTATTARCAAA (p.Asn174Ser) TTGGACCGAATGGGCTCCAAC′] 3359 NM_024301.4(FKRP): c.926A > G 79147 FKRP [′GTGGGCGACACGCCCGCCTACCTCTRCGA (p.Tyr309Cys) GGAGCGCTGGACGCCCCCCTGC′] 3360 NM_024301.4(FKRP): c.1387A > G 79147 FKRP [′TGCCGGCTTCGTGGCGCAGGCGCCTRACA (p.Asn463Asp) ACTACCGCCGCTTCCTGGAGCT′] 3361 NM_021020.3(LZTS1): c.355A > G 11178 LZTS1 [′TCCTTTTCCTCTACAGGGCTCCGAGRAGGG (p.Lys119Glu) TGCAGTGAGGCCCACAGCCTT′] 3362 NM_001005741.2(GBA): c.1226A > 2629 GBA [′CCAGCCGACCACATGGTACAGGAGGBTCT G (p.Asn409Ser) AGGGTAAGGACAAAGGCAAAGA′] 3363 NM_000157.3(GBA): c.680A > G 2629 GBA [′ACATCACCCACTTGGCTCAAGACCARTGG (p.Asn227Ser) AGCGGTGAATGGGAAGGGGTCA′] 3364 NM_001005741.2(GBA): c.1049A > 2629 GBA [′AAATATGTTCATGGCATTGCTGTACRTTGG G (p.His350Arg) TACCTGGACTTTCTGGCTCCA′] 3365 NM_014239.3(E1F2B2): c.638A > G 8892 EIF2B2 [′GTGAATTTGTCCAAAGCAGGTATTGRGAC (p.Glu213Gly) AACTGTCATGACTGATGCTGCC′] 3366 NM_015915.4(ATL1): c.773A > G 51062 ATL1 [′CTACAGAACGTCAGAAAACACATCCRTTC (p.His258Arg) CTGTTTCACCAACATTTCCTGT′] 3367 NM_015915.4(ATL1): c.1222A > G 51062 ATL1 [′GAAGCTATTCCGAGGGGTGAAGAAGRTGG (p.Met408Val) GTGGGGAAGAATTTAGCCGGCG′] 3368 NM_022051.2(EGLN1): c.1121A > G 54583 EGLN1 [′TTCTGGTCTGACCGTCGCAACCCTCRTGAA (p.His374Arg) GTACAACCAGCATATGCTACA′] 3369 NM_015629.3(PRPF31): c.527 + 3A > 26121 PRPF31 [′GTCACCGCCTCCACCACCCAGGGGTRTGTC G CGCTTCGAGGGAGGCGCCGGG′] 3370 NM_001243133.1(NLRP3): c.1880A > 114548 NLRP3 [′CTGCAGATCCAGCCCAGCCAGCTGGRATT G (p.Glu627Gly) GTTCTACTGTTTGTACGAGATG′] 3371 NM_020919.3(ALS2): c.2980 − 57679 ALS2 [′CTTATTGACGTTACTTTTTGCTCCTRGACA 2A > G AAGTGGCTACGAGCTATAAGC′] 3372 NM_175073.2(APTX): c.602A > G 54840 APTX [′GATAAATACCCAAAGGCCCGTTACCRTTG (p.His201Arg) GCTGGTCTTACCGTGGACCTCC′] 3373 NM_001002010.2(NT5C3A): c.686A > 51251 NT5C3A [′CATCCCAATGTCAAAGTTGTGTCCARTTTT G (p.Asn229Ser) ATGGATTTTGATGAAACTGTA′] 3374 NM_020451.2(SEPN1): c.1A > G 57190 SEPN1 [′CCGGCAGCCGCCGCCAGCCGCAGCCRTGG (p.Met1Val) GCCGGGCCCGGCCGGGCCAACG′] 3375 NM_032667.6(BSCL2): c.263A > G −1 — [′TCACTCTGCTCCTTCCCTGTTGCCARTGTCT (p.Asn88Ser) CGCTGACTAAGGGTGGACGT′] 3376 NM_153638.2(PANK2): c.700A > G 80025 PANK2 [′GGTATATTTTGAACCCAAAGACATCRCTGC (p.Thr234Ala) TGAAGAAGAAGAGGAAGAAGT′] 3377 NM_025243.3(SLCl9A3): c.1264A > 80704 SLC19A3 [′TGCCTTGGTGATTCAGACCATCATGRCTGT G (p.Thr422Ala) GATTGTAGTAGATCAGAGAGG′] 3378 NM_025243.3(SLCl9A3): c.130A > 80704 SLC19A3 [′TATCCCATATTTATCTGGACCAGATRAAAA G (p.Lys44Glu) CCTGACCAGTGCAGAGGTAAG′] 3379 NM_031885.3(BBS2): c.209A > G 583 BBS2 [′CAGACAGCTGACTGCCTGGTTAATGYTGA (p.Asn70Ser) GAAGAGAAACATCAGATTCCAG′] 3380 NM_032383.4(HPS3): c.2482 − 2A > G −1 — [′TTTATGAGAAATTCTTTTATGTTTTRGATA AATGCCTGTAGTCATTATGGC′] 3381 NM_021830.4(C10orf2): c.955A > G 56652 C10orf2 [′TGACCTTCGGTCCTGGGAAGCCGCCVAGTT (p.Lys319Glu) GTTTGCACGAAAACTGAACCC′] 3382 NM_021830.4(C10orf2): c.1523A > 56652 C10orf2 [′ACAATGCAACATGCAGTCTACGTCTRTGAC G (p.Tyr508Cys) ATTTGTCATGTGATCATCGAC′] 3383 NM_006492.2(ALX3): c.608A > G 257 ALX3 [′ACCTGCCCCCAGGTCTGGTTCCAGARCCGC (p.Asn203Ser) AGAGCCAAGTGGCGGAAGCGC′] 3384 NM_174916.2(UBR1): c.407A > G 197131 UBR1 [′TTCCAGGACAGTGTTCATAAAAATCRTCGT (p.His136Arg) TACAAGGTAAGAAAATATAAC′] 3385 NM_015166.3(MLC1): c.422A > G 23209 MLC1 [′CTAGTCCTGAACCCATCAGCAATAARCGT (p.Asn141Ser) GAGTTCACACGAGCTTCGCGCA′] 3386 NM_013391.3(DMGDH): c.326A > 29958 DMGDH [′CCTGGAATAAACTTGAAGAAAATACRTTA G (p.His109Arg) TGATAGCATCAAACTTTATGAG′] 3387 NM_014795.3(ZEB2): c.3356A > G 9839 ZEB2 [′GCTTACTTGCAGAGCATTACCCCTCRGGGG (p.Gln1119Arg) TACTCTGACTCGGAGGAGAGG′] 3388 NM_000441.1(SLC26A4): c.1151A > 5172 SLC26A4 [′GTTGTCATCCAGTCTCTTCCTTAGGRATTC G (p.Glu384Gly) ATTGCCTTTGGGATCAGCAAC′] 3389 NM_000441.1(SLC26A4): c.1105A > 5172 SLC26A4 [′TTATGCTATTGCAGTGTCAGTAGGARAAGT G (p.Lys369Glu) ATATGCCACCAAGTATGATTA′] 3390 NM_000441.1(SLC26A4): c.2168A > 5172 SLC26A4 [′AAGGACACATTCTTTTTGACGGTCCRTGAT G (p.His723Arg) GCTATACTCTATCTACAGAAC′] 3391 NM_000441.1(SLC26A4): c.919 − 5172 SLC26A4 [′TTTTTAACATCTTTTGTTTTATTTCRGACGA 2A > G TAATTGCTACTGCCATTTCA′] 3392 NM_000197.1(HSD17B3): c.703A > 3293 HSD17B3 [′GACCCCATATGCTGTCTCGACTGCARTGAC G (p.Met235Val) AAAGTATCTAAATACAAATGT′] 3393 NM_000197.1(HSD17B3): c.389A > 3293 HSD17B3 [′TTGTTTTGCTTCTTTCTGCCAGTCARCAATG G (p.Asn130Ser) TCGGAATGCTTCCAAACCTT′] 3394 NM_022458.3(LMBR1): c.423 + 5252A > 64327 LMBR1 [′GTTTTGATCTTAGTGTTTATTACAGRAAAT G GAAGCCATATCTCACTAACTA′] 3395 NM_020366.3(RPGRIP1): c.3341A > 57096 RPGRIP1 [′CTAAATAAACATTTTCCTTATCAGGRTTCA G (p.Asp1114Gly) GAGAAGATGTGCATTGAAATT′] 3396 NM_021625.4(TRPV4): c.998A > G 59341 TRPV4 [′CTGCATGCGCTGGTGGCCATTGCTGRCAAC (p.Asp333Gly) ACCCGTGAGAACACCAAGTTT′] 3397 NM_004897.4(MINPP1): c.809A > G 9562 MINPP1 [′TTAAAAAAAGTTGCAGCTACTTTGCRAGTG (p.Gln270Arg) CCAGTAAATGATTTAAATGCA′] 3398 NM_020638.2(FGF23): c.211A > G 8074 FGF23 [′TGGCGCACCCCATCAGACCATCTACRGTG (p.Ser71Gly) AGTAGGGCTTCAGGCTGGGAAG′] 3399 NM_015071.4(ARHGAP26): c.1250A > 23092 ARHGAP26 [′GGGCTGTATCGAATTGTGGGTGTCAVCTCC  G (p.Asn417Ser) AGAGTGCAGAAGTTGCTGAGT′] 3400 NM_021615.4(CHST6): c.521A > G 4166 CHST6 [′CGCTCCTACAGCCACGTGGTGCTCARGGA (p.Lys174Arg) GGTGCGCTTCTTCAACCTGCAG′] 3401 NM_021615.4(CHST6): c.329A > G 4166 CHST6 [′TGCGACATGGACGTGTTTGATGCCTRTCTG (p.Tyr110Cys) CCTTGGCGCCGCAACCTGTCC′] 3402 NM_015560.2(OPA1): c.1745A > G 4976 OPA1 [′CTTGAAACTGAATGGAAGAATAACTRTCC (p.Tyr582Cys) TCGCCTGCGGGAACTTGACCGG] 3403 NM_000368.4(TSC1): c.1760A > G 7248 TSC1 [′AGTATCTTCACTCCCAGTCCTTGTARAATT (p.Lys587Arg) CCACCTCCGACGAGAGTGGGC′] 3404 NM_020661.2(AICDA): c.415A > G 57379 AICDA [′CCGCGCCGGGGTGCAAATAGCCATCRTGA (p.Met139Val) CCTTCAAAGGTGCGAAAGGGCC′] 3405 NM_020533.2(MCOLN1): c.406 − 57192 MCOLN1 [′ACAGGCCCTCCCCTTCTCTGCCCACRGTAC 2A > G CTGGCGTTGCCTGACGTGTCA′] 3406 NM_021102.3(SPINT2): c.488A > G 10653 SPINT2 [′AGGAACTCCTGCAATAACTTCATCTRTGGA (p.Tyr163Cys) GGCTGCCGGGGCAATAAGAAC′] 3407 NM_006610.3(MASP2): c.359A > G 10747 MASP2 [′GGGAGGGCTGGGGTTTCTCAGGGTCRGGG - (p.Asp120Gly) GGTCCCCAAGGAGTAGCCAGGG′, 3410 ′AGGGGCCAGGCTTGGCCAGGAGGGASATC AGGCCTGGGTCTTGCCTTCACT′, ′GGGCAGGAGAGCACAGACACGGAGCRGGC CCCTGGCAAGGACACTTTCTAC′, ′AGCCTGGACATTACCTTCCGCTCCGRCTAC TCCAACGAGAAGCYGTTCACG′] 3411 NM_018965.3(TREM2): c.401A > G 54209 TREM2 [′GCTCTGCCTCCCATAGACCCCCTGGRTCAC (p.Asp134Gly) CGGGATGCTGGAGATCTCTGG′] 3412 NM_007375.3(TARDBP): c.1009A > 23435 TARDBP [′AGCACTACAGAGCAGTTGGGGTATGRTGG G (p.Met337Val) GCATGTTAGCCAGCCAGCAGAA′] 3413 NM_007375.3(TARDBP): c.506A > 23435 TARDBP [′GTAATGTCACAGCGACATATGATAGRTGG G (p.Asp169Gly) ACGATGGTGTGACTGCAAACTT′] 3414 NM_007375.3(TARDBP): c.1028A > 23435 TARDBP [′GGTATGATGGGCATGTTAGCCAGCCRGCA G (p.Gln343Arg) GAACCAGTCAGGCCCATCGGGT′] 3415 NM_007375.3(TARDBP): c.787A > 23435 TARDBP [′CGTTCATATATCCAATGCCGAACCTRAGCA G (p.Lys263Glu) CAATAGCAATAGACAGTTAGA′] 3416 NM_015247.2(CYLD): c.2240A > G 1540 CYLD [′ATCAACAGTAACCTGAAATTTGCAGRGGT (p.Glu747Gly) TAGTGATACTCACCTGTGGTAT′] 3417 NM_001128425.1(MUTYH): c.536A > 4595 MUTYH [′AATCAACTCTGGGCTGGCCTGGGCTRCTAT G (p.Tyr179Cys) TCTCGTGGCCGGCGGCTGCAG′] 3418 NM_001128425.1(MUTYH): c.1241A > 4595 MUTYH [′TCCGTGACCTGGGAGCCCTCAGAGCRGCTT G (p.Gln414Arg) CAGCGCAAGGCCCTGCTGCAG′] 3419 NM_170784.2(MKKS): c.110A > G 8195 MKKS [′TTGAAAAGAATTGTAACATCATGCTRTGGC (p.Tyr37Cys) CCCTCAGGTAGGCTGAAGCAG′] 3420 NM_170784.2(MKKS): c.169A > G 8195 MKKS [′TGGCTTTGGAGGTTACGTGTGTACARCCTC (p.Thr57Ala) ACAGTCCTCAGCTCTGCTCAG′] 3421 NM_004795.3(KL): c.578A > G 9365 KL [′GTGCAGCCCGTGGTCACCCTGTACCRCTGG (p.His193Arg) GACCTGCCCCAGCGCCTGCAG′] 3422 NM_014625.3(NPHS2): c.479A > G 7827 NPHS2 [′CTTTTCTTTTTTTTGCCCTGCCTGGRTACCT (p.Asp160Gly) ACCACAAGGTTGACCTTCGT′] 3423 NM_015713.4(RRM2B): c.322 − 50484 RRM2B [′CACCTCCTGACTAAAGCGCTCCACCBAAG 2A > G AAGATAAGGAAAATAGATATAT′] 3424 NM_006343.2(MERTK): c.1605 − 10461 MERTK [′GAAGTATCTTTGTTTTCATTCACCCRGGAA 2A > G TGCATTCACAGAGGAGGATTC′] 3425 NM_014585.5(SLC40A1): c.470A > 30061 SLC40A1 [′GCTACTGCAATCACAATCCAAAGGGRTTG G (p.Asp157Gly) GATTGTTGTTGTTGCAGGAGAA′] 3426 NM_004924.4(ACTN4): c.763A > G 81 ACTN4 [′CGTGAACACGGCCCGGCCCGACGAGRAGG (p.Lys255Glu) CCATAATGACCTATGTGTCCAG′] 3427 NM_012193.3(FZD4): c.1024A > G −1 — [′CAAATGGGGTCATGAAGCCATTGAARTGC (p.Met342Val) ACAGCTCTTATTTCCACATTGC′] 3428 NM_012193.3(FZD4): c.766A > G −1 — [′GTTTTCCTACCCTGAGCGCCCCATCRTATT (p.Ile256Val) TCTCAGTATGTGCTATAATAT′] NM_004239.3(TRIP11): c.202 − 9321 TRIP11 2A > G 3429 NM_004239.3(TRIP11): c.2102A > G 9321 TRIP11 [′AGTGTTTTTTTCCAGAGAAAGCTGAYTGTT (p.Asn701Ser) ACCAGCAAGACATTCTTCTAA′] 3430 NM_012434.4(SLC17A5): c.548A > 26503 SLC17A5 [′CAGGGTGTTACATTTCCAGCCATGCRTGCC G (p.His183Arg) ATGTGGTCTTCTTGGGCTCCC′] 3431 NM_001099274.1(TINF2): c.838A > 26277 TINF2 [′ATGGGCCTCCACTAGGGGAGGCCATDAGG G (p.Lys280Glu) AGCGCCCCACAGTCATGCTGTT′] 3432 NM_012415.3(RAD54B): c.1778A > 25788 RAD54B [′AGAGTTGAACAAAAGGCAGGGGTGAYTGC G (p.Asn593Ser) ACAGTTTTTTAAGAGCTCCTAT′] 3433 NM_014946.3(SPAST): c.1688 − 6683 SPAST [′TAAGTGCCTGACTTTTATGTTTTACRGAAC 2A > G TAAAACCAGAACAGGTGAAGA′] 3434 NM_014946.3(SPAST): c.1322A > G 6683 SPAST [′AGTCTTATACTTGTATTTCCTCTAGRTGAA (p.Asp441Gly) GTTGATAGCCTTTTGTGTGAA′] 3435 NM_014946.3(SPAST): c.1245 + 4A > 6683 SPAST [′TGCAAGTTTAACTTCAAAATACGTGRGTGC G TCTGTTTCCAATATTGTCGTA′] 3436 NM_014946.3(SPAST): c.1157A > G 6683 SPAST [′CTGTTACTCTTTGGTCCACCTGGGARTGGG (p.Asn386Ser) AAGACAATGCTGGTAAGGGTT′] 3437 NM_014946.3(SPAST): c.1216A > G 6683 SPAST [′AGAATCGAATGCAACCTTCTTTAATRTAAG (p.Ile406Val) TGCTGCAAGTTTAACTTCAAA′] 3438 NM_182643.2(DLC1): c.2875A > G 10395 DLC1 [′ACACCTGGATGTGGACAACGACCGARCCA (p.Thr959Ala) CACCCAGCGACCTGGACAGCAC′] 3439 NM_194456.1(KRIT1): c.410A > G 889 KRIT1 [′TGCCCAATTTTTTACTGCTTACAAGRTATT (p.Asp137Gly) ATGCGAGTCTGTAGTGAATCC′] 3440 NM_005094.3(SLC27A4): c.899A > 10999 SLC27A4 [′CCAGGAAACATCGTGGGAATCGGCCRGTG G (p.Gln300Arg) CCTGCTGCATGGCATGACGGTG′] 3441 NM_004211.3(SLC6A5): c.1472A > 9152 SLC6A5 [′GGAGGCCTGATCACTCTCTCTTCTTRCAAC G (p.Tyr491Cys) AAATTCCACAACAACTGCTAC′] 3442 NM_004211.3(SLC6A5): c.1526A > 9152 SLC6A5 [′GACACTCTAATTGTCACCTGCACCARCAGT G (p.Asn509Ser) GCCACAAGCATCTTTGCCGGC′] 3443 NM_014270.4(SLC7A9): c.695A > G 11136 SLC7A9 [′GCGTTTTACAATGGACTCTGGGCCTRTGAT (p.Tyr232Cys) GGATGGTGAGGTGTCCTGAGA′] 3444 NM_139025.4(ADAMTS13): c.1582A > 11093 ADAMTS13 [′GAGCCTGTGTGTGTCGGGCAGCTGCRGGG G (p.Arg528Gly) TAGGCGTGTGTGGACATTGGCG′] 3445 NM_002894.2(RBBP8): c.1009A > G 5932 RBBP8 [′TGTATTTGGAGCTACCTCTAGTATCRAAAG (p.Lys337Glu) TGGTTTAGATTTGAATACAAG′] 3446 NM_005682.6(ADGRGl): c.263A > 9289 ADGRGl [′TCCTTCCCTGACCCCAGGGGCCTCTRCCAC G (p.Tyr88Cys) TTCTGCCTCTACTGGAACCGA′] 3447 NM_006502.2(POLH): c.1603A > G 5429 POLH [′AGGAACTGAGCCCTTCTTTAAGCAGRAAA (p.Lys535Glu) GTCTGCTTCTAAAGCAGAAACA′] 3448 NM_000334.4(SCN4A): c.4774A > G 6329 SCN4A [′CATCTCCTTCCTCATCGTGGTCAACRTGTA (p.Met1592Val) CATCGCCATCATCCTGGAGAA′] 3449 NM_000334.4(SCN4A): c.3478A > G 6329 SCN4A [′CGCCCTCCTAGGCGCCATCCCCTCCRTCAT (p.Ile1160Val) GAATGTGCTGCTTGTCTGCCT′] 3450 NM_000334.4(SCN4A): c.421A > G 6329 SCN4A [′GTTCAGCATGTTCATCATGATCACCRTCTT (p.Ile141Val) GACCAACTGCGTATTCATGAC′] 3451 NM_003907.2(EIF2B5): c.271A > G 8893 EIF2B5 [′CCTGACTGCCACAGGTGTACAGGAARCAT (p.Thr91Ala) TTGTCTTTTGTTGCTGGAAAGC′] 3452 NM_001128227.2(GNE): c.604A > G 10020 GNE [′CAGTGCAGAGCAGCACCTGATATCCRTGT (p.Met202Val) GTGAGGACCATGATCGCATCCT′] 3453 NM_001029.3(RPS26): c.1A > G 6231 RPS26 [′CCTCTCTCCGGTCCGTGCCTCCAAGDTGGT (p.Met1Val) GAGTCTTCTTGCGTGGTGAGG′] 3454 NM_012082.3(ZFPM2): c.1969A > G −1 — [′TCAAACTAAGAAGCTCTCCACCTCCRGTAA (p.Ser657Gly) CAATGATGACAAAATTAATGG′] 3455 NM_012082.3(ZFPM2): c.89A > G 23414 ZFPM2 [′GATGAGGAAGAAGAATGTCCATCAGRGGA (p.Glu30Gly) AACAGACATCATCTCCAAAGGA′] 3456 NM_012082.3(ZFPM2): c.2527A > G −1 — [′AAAGTGTTTATCTCAGTCTGAGCGGRCGAC (p.Thr843Ala) CACGTCTCCCAAAAGGCTGCT′] 3457 NM_194248.2(OTOF): c.766 − 2A > G 9381 OTOF [′TCTGGCTCCCCCTTCTCCTGCCTGCRGGTC AGCATCACGGTGATCGAGGCC′] 3458 NM_003705.4(SLC25A12): c.1769A > 8604 SLC25A12 [′GCTCGAGTGTTTCGATCCTCTCCCCRGTTT G (p.Gln590Arg) GGTGTTACCTTGGTCACTTAT′] 3459 NM_004621.5(TRPC6): c.428A > G 7225 TRPC6 [′AATGCCCTACAGTTGGCAGTGGCCARTGA (p.Asn143Ser) GCATCTGGAAATTACAGAACTT′] 3460 NM_152384.2(BBS5): c.522 + 3A > G 129880 BBS5 [′TTTGGAATTTATCCAGTGATCAGGTRTTGT GCAAAGAGCTAGTGAACCTTT′] 3461 NM_152384.2(BBS5): c.547A > G 129880 BBS5 [′GGGCAATTTAGGAACCTTTTTTATTRCCAA (p.Thr183Ala) TGTGAGAATTGTGTGGCATGC′] 3462 NM_001257342.1(BCS1L): c.232A > 617 BCS1L [′CCACAGTACCCGTACTCAGCACCTCRGTGT G (p.Ser78Gly) CGAGACTTCGTACCTTCAGCA′] 3463 NM_004328.4(BCS1L): c.148A > G 617 BCS1L [′GGCATTCCGGCGCCATTACATGATCRCACT (p.Thr50Ala) GGAAGTCCCTGCTCGAGACAG′] 3464 NM_003839.3(TNFRSF11A): c.508A > 8792 TNFRSF11A [′TGCCTTTTCCTCCACGGACAAATGCRGACC G (p.Arg170Gly) CTGGACCAAGTAAGTAACAAC′] 3465 NM_030761.4(WNT4): c.647A > G 54361 WNT4 [′TGCCACGGGGTGTCAGGCTCCTGTGRGGT (p.Glu216Gly) AAAGACGTGCTGGCGAGCCGTG′] 3466 NM_022817.2(PER2): c.1984A > G 8864 PER2 [′GCTGGCACTGCCGGGCAAGGCAGAGRGTG (p.Ser662Gly) TGGCGTCGCTCACCAGCCAGTG′] 3467 NM_002977.3(SCN9A): c.1964A > G −1 — [′TAGGGCACGACCAATCAAATACACARGAA (p.Lys655Arg) AAGGCGTTGTAGTTCCTATCTC′] 3468 NM_002977.3(SCN9A): c.184A > G 6335 SCN9A [′GGAAGCTGGCAAACAGCTGCCCTTCRTCT (p.Ile62Val) ATGGGGACATTCCTCCCGGCAT′] 3469 NM_002977.3(SCN9A): c.29A > G 6335 SCN9A [′GCAATGTTGCCTCCCCCAGGACCTCRGAGC (p.Gln10Arg) TTTGTCCATTTCACAAAACAG′] 3470 NM_001457.3(FLNB): c.604A > G 2317 FLNB [′GCCTGTGGATAATGCACGAGAAGCCRTGC (p.Met202Val) AGCAGGCAGATGACTGGCTGGG′] 3471 NM_003060.3(SLC22A5): c.632A > 6584 SLC22A5 [′GTAGGCATGGGCCAGATCTCCAACTRTGT G (p.Tyr211Cys) GGCAGCATTTGTCCTGGGTATG′] 3472 NM_000369.2(TSHR): c.1856A > G 7253 TSHR [′CCGCAGTACAACCCAGGGGACAAAGRTAC (p.Asp619Gly) CAAAATTGCCAAGAGGATGGCT′] 3473 NM_000369.2(TSHR): c.548A > G 7253 TSHR [′TCTCTTCTCTCTGTTGGTTGTAGGARGCTG (p.Lys183Arg) TACAACAATGGCTTTACTTCA′] 3474 NM_001430.4(EPAS1): c.1603A > G 2034 EPAS1 [′GGAGACACTGGCACCCTATATCCCCRTGG (p.Met535Val) ACGGGGAAGACTTCCAGCTAAG′] 3475 NM_024009.2(GJB3): c.421A > G 2707 GJB3 [′GTTCAGCCTCATCTTCAAGCTCATCRTTGA (p.Ile141Val) GTTCCTCTTCCTCTACCTGCT′] 3476 NM_024009.2(GJB3): c.497A > G 2707 GJB3 [′ATGCCGCGCCTGGTGCAGTGTGCCADCGT (p.Asn166Ser) GGCCCCCTGCCCCAACATCGTG′] 3477 NM_001080463.1(DYNC2H1): c.11284A > 79659 DYNC2H1 [′TGTTTTTTTGCTTTTGTAGGTTGCCRTGGGT G (p.Met3762Val) CAAGGTCAAGCTGATTTAGC′] 3478 NM_001080463.1(DYNC2H1): c.9044A > 79659 DYNC2H1 [′CGCATGCCACCTGATGTAATTAGAGRTATT G (p.Asp3015Gly) CTTGAAGGAGTTTTAAGGTTG′] 3479 NM_001080463.1(DYNC2H1): c.4610A > 79659 DYNC2H1 [′GTTGACCCATCTCTGTTCCCTTCACRGGTA G (p.Gln1537Arg) AGGGGGCTTACGTGTAGAAGC′] 3480 NM_001080463.1(DYNC2H1): c.5959A > 79659 DYNC2H1 [′TGGTCCAAGTGGTGCTGGAAAATCARCGC G (p.Thr1987Ala) TTTGGAGAATGTTAAGGGCTGC′] 3481 NM_007035.3(KERA): c.740A > G 11081 KERA [′GTGGCCTTTTTGAGACTAAATCACARCAAA (p.Asn247Ser) CTGTCAGATGAGGGTCTCCCA′] 3482 NM_003722.4(TP63): c.697A > G 8626 TP63 [′TATCCGCGCCATGCCTGTCTACAAARAAGC (p.Lys233Glu) TGAGCACGTCACGGAGGTGGT′] 3483 NM_003722.4(TP63): c.1052A > G 8626 TP63 [′CGGATCTGTGCTTGCCCAGGAAGAGRCAG (p.Asp351Gly) GAAGGCGGATGAAGATAGCATC′] 3484 NM_003722.4(TP63): c.1054A > G 8626 TP63 [′GATCTGTGCTTGCCCAGGAAGAGACRGGA (p.Arg352Gly) AGGCGGATGAAGATAGCATCAG′] 3485 NM_003742.2(ABCB11): c.890A > 8647 ABCB11 [′AGAACAGTGGCTGCTTTTGGTGGTGRGAA G (p.Glu297Gly) AAGAGAGGTTGAAAGGTTGGTT′] 3486 NM_006702.4(PNPLA6): c.3034A > 10908 PNPLA6 [′GGACCTCACGTACCCAGTCACCTCCRTGTT G (p.Met1012Val) CACTGGGTCTGCCTTTAACCG′] 3487 NM_006412.3(AGPAT2): c.589 − 10555 AGPAT2 [′GTACACCACGGGGACGATGGGCACCYGCA 2A > G GGCAGGGAGACGCACAGCTGAG′] 3488 NM_001084.4(PLOD3): c.668A > G 8985 PLOD3 [′AAGTCTCGGATCTTTCAGAACCTCARCGGG (p.Asn223Ser) GCTTTAGGTGAGGAGGAAGAC′] 3489 NM_138554.4(TLR4): c.896A > G 7099 TLR4 [′GCATACTTAGACTACTACCTCGATGRTATT (p.Asp299Gly) ATTGACTTATTTAATTGTTTG′] 3490 NM_001130978.1(DYSF): c.3892A > 8291 DYSF [′GGGCCAGGTGCAGGAGACATCAAGGRTCC G (p.Ile1298Val) TGGATGAGGTGAGCTGGGCGTG′] 3491 NM_001130978.1(DYSF): c.5264A > 8291 DYSF [′CTTTTTTGTCTTCTCTCTGGGGCAGRGGCT G (p.Glu1755Gly) GGCAGGATCCCAAACCCACAC′] 3492 NM_003494.3(DYSF): c.3443 − 8291 DYSF [′CAGCTCTTAACCACTCCAGCCACTCRCTCT 33A > G GGCACCTCTGTTTTTTCCCTT′] 3493 NM_003494.3(DYSF): c.1285 − 8291 DYSF [′AACTTGTCCCCTCCCTGTGTCTTCTRGCTGT 2A > G GCAGCAAGATCTTGGAGAAG′] 3494 NM_005450.4(NOG): c.665A > G 9241 NOG [′CGCTGCGGCTGGATTCCCATCCAGTRCCCC (p.Tyr222Cys) ATCATTTCCGAGTGCAAGTGC′] 3495 NM_004629.1(FANCG): c.925 − 2189 FANCG [′GATACAATTTTTTTCTTTCTCTTTTRGGCCT 2A > G TGAATGTCCCATGCAGTTCC′] 3496 NM_001701.3(BAAT): c.226A > G 570 BAAT [′AGGGGATTATATGGGAGTCCACCCCRTGG (p.Met76Val) GTCTCTTCTGGTCTCTGAAACC′] 3497 NM_006892.3(DNMT3B): c.2450A > 1789 DNMT3B [′TTTGGCTTTCCTGTGCACTACACAGRCGTG G (p.Asp817Gly) TCCAACATGGGCCGTGGTGCC′] 3498 NM_001360.2(DHCR7): c.839A > G 1717 DHCR7 [′GCTGCTTCTGTCTTGCAGGCCATCTRCGTG (p.Tyr280Cys) ATTGACTTCTTCTGGAACGAA′] 3499 NM_001360.2(DHCR7): c.1A > G 1717 DHCR7 [′AAGGTTCTCTTTCTTGCAGGGCCCARTGGC (p.Met1Val) TGCAAAATCGCAACCCAACAT′] 3500 NM_032119.3(ADGRV1): c.18131A > 84059 ADGRV1 [′TATCATCAGAGCATGTCACAGATCTRTGGA G (p.Tyr6044Cys) CTCATTCATGGTGACCTGTAA′] 3501 NM_004984.2(KIF5A): c.767A > G 3798 KIF5A [′GTGCTGGACGAGGCAAAGAATATCARCAA (p.Asn256Ser) GTCACTGTCAGCTCTGGGCAAT′] 3502 NM_004984.2(KIF5A): c.827A > G 3798 KIF5A [′GTCTCCTTCCTCCCCCAGAAAAGCTRTGTT (p.Tyr276Cys) CCATATCGTGACAGCAAAATG′] 3503 NM_007373.3(SHOC2): c.4A > G 8036 SHOC2 [′TTTTGTCCAGGCTTGAGTCACCATGRGTAG (p.Ser2Gly) TAGTTTAGGAAAAGAAAAAGA′] 3504 NM_016203.3(PRKAG2): c.1148A > 51422 PRKAG2 [′TACTCCTTGATCAAAAATAAAATCCRCAG G (p.His383Arg) ATTGCCCGTTATTGACCCTATC′] 3505 NM_016203.3(PRKAG2): c.1589A > 51422 PRKAG2 [′GTCCTGCTTTTCTCTTTGCAGGTCCRTCGG G (p.His530Arg) CTGGTGGTGGTAAATGAAGCA′] 3506 NM_003816.2(ADAM9): c.411 − 8754 ADAM9 [′TTAAAATTTGTATACGTGTAATGCARCATT 8A > G CAGAGGATTGCTGCATTTAGA′] 3507 NM_003002.3(SDHD): c.341A > G 6392 SDHD [′GGCCTTGGACAAGTTGTTACTGACTRTGTT (p.Tyr114Cys) CATGGGGATGCCTTGCAGAAA′] 3508 NM_003002.3(SDHD): c.149A > G 6392 SDHD [′GAATGGTGTGGAGTGCAGCACATACRCTT (p.His50Arg) GTCACCGAGCCACCATTGTATG′] 3509 NM_003002.3(SDHD): c.1A > G 6392 SDHD [′ATGACCTTGAGCCCTCAGGAACGAGDTGG (p.Met1Val) CGGTTCTCTGGAGGCTGAGTGC′] 3510 NM_002485.4(NBN): c.511A > G 4683 NBN [′TCAGTAAAATATTCTGGCTTTACAAYTGGA (p.Ile171Val) CGTCCACAAATGAGTGCACAT′] 3511 NM_170695.3(TGIF1): c.838A > G 7050 TGIF1 [′TTCCTGTACAGCTGGGCCAAACCCARCCCT (p.Thr280Ala) AGGGAGGCCACTGTCTCCTAA′] 3512 NM_002408.3(MGAT2): c.785A > G 4247 MGAT2 [′CTTATACTTTTCCTAGAAGAGGATCRCTAC (p.His262Arg) TTAGCCCCAGACTTTTACCAT′] 3513 NM_002408.3(MGAT2): c.952A > G 4247 MGAT2 [′GAAAACTTGGAAATCCACAGAGCACRATA (p.Asn318Asp) TGGGTCTAGCCTTGACCCGGAA′] 3514 NM_005422.2(TECTA): c.5609A > 7007 TECTA [′CAGTCCAATGGCACGCATATCATGTRTAA G (p.Tyr1870Cys) AAACACACTCTGGATCGAAAGC′] 3515 NM_012233.2(RAB3GAP1): c.649 − 22930 RAB3GAP1 [′GGTATTGTCTTTGCATGTATTTCCTRGGGA 2A > G TGTCCTTTAACTCCATTGCCT′] 3516 NM_005025.4(SERPINI1): c.1013A > 5274 SERPINI1 [′GAGATTTTTCTTTCCAAAGCAATTCRCAAG G (p.His338Arg) TCCTTCCTAGAGGTTAATGAA′] 3517 NM_001040667.2(HSF4): c.256A > 3299 HSF4 [′AGACGGTTTTCGGAAGGTGGTGAGCRTCG G (p.Ile86Val) AGCAGGGCGGCCTGCTTAGGCC′] 3518 NM_001008211.1(OPTN): c.1433A > 10133 OPTN [′GTTTACTGTTCTGATTTTCATGCTGRAAGA G (p.Glu478Gly) GCAGCGAGAGAGAAAATTCAT′] 3519 NM_000492.3(CFTR): c.2738A > G 1080 CFTR [′GTGATTATCACCAGCACCAGTTCGTRTTAT (p.Tyr913Cys) GTGTTTTACATTTACGTGGGA′] 3520 NM_000492.3(CFTR): c.3717 + 4A > 1080 CFTR [′CTCAATAAGTCCTGGCCAGAGGGTGRGAT G TTGAACACTGCTTGCTTTGTTA′] 3521 NM_000492.3(CFTR): c.1666A > G 1080 CFTR [′ACTGAGTGGAGGTCAACGAGCAAGARTTT (p.Ile556Val) CTTTAGCAAGGTGAATAACTAA′] 3522 NM_000492.3(CFTR): c.326A > G 1080 CFTR [′TTACTGGGAAGAATCATAGCTTCCTRTGAC (p.Tyr109Cys) CCGGATAACAAGGAGGAACGC′] 3523 NM_000492.3(CFTR): c.273 + 4A > G 1080 CFTR [′TGGAATCTTTTTATATTTAGGGGTARGGAT CTCATTTGTACATTCATTATG′] 3524 NM_000492.3(CFTR): c.3254A > G 1080 CFTR [′CTGTTCCACAAAGCTCTGAATTTACRTACT (p.His1085Arg) GCCAACTGGTTCTTGTACCTG′] 3525 NM_000492.3(CFTR): c.3700A > G 1080 CFTR [′CATATTAGAGAACATTTCCTTCTCANTAAG (p.Ile1234Val) TCCTGGCCAGAGGGTGAGATT′] 3526 NM_000492.3(CFTR): c.650A > G 1080 CFTR [′GCACTCCTCATGGGGCTAATCTGGGRGTTG (p.Glu217Gly) TTACAGGCGTCTGCCTTCTGT′] 3527 NM_001005360.2(DNM2): c.1684A > 1785 DNM2 [′CCTCCACCCTCAGGAGAAAGAGAAGRAGT G (p.Lys562Glu) ACATGCTGCCTCTGGACAACCT′] 3528 NM_001814.4(CTSC): c.857A > G 1075 CTSC [′TCTCAGACCCCAATCCTAAGCCCTCRGGAG (p.Gln286Arg) GTTGTGTCTTGTAGCCAGTAT′] 3529 NM_001814.4(CTSC): c.1235A > G 1075 CTSC [′AATCATGCTGTTCTGCTTGTGGGCTRTGGC (p.Tyr412Cys) ACTGACTCAGCCTCTGGGATG] 3530 NM_001814.4(CTSC): c.1040A > G 1075 CTSC [′CGTTATTACTCCTCTGAGTACCACTRTGTA (p.Tyr347Cys) GGAGGTTTCTATGGAGGCTGC′] 3531 NM_005144.4(HR): c.-218A > G 55806 HR [′TCCGACCCCTCCAACCTGCGGCCCTRGAGC GCCCCCGCCGCCCCGGGGGAA′] 3532 NM_172107.2(KCNQ2): c.851A > G 3785 KCNQ2 [′ACCACCATTGGCTACGGGGACAAGTRCCC (p.Tyr284Cys) CCAGACCTGGAACGGCAGGCTC′] 3533 NM_001303.3(COX10): c.1007A > G 1352 COX10 [′GCCCTGAGCTGGGGCCTCCGTGAAGDCTA (p.Asp336Gly) CTCCCGGGGCGGCTACTGCATG′] 3534 NM_006214.3(PHYH): c.135 − 2A > G 5264 PHYH [′AGAACGTTATTATCCAGAGTATACCYAAA GGAGAAAAAGAATCCCAAAATA′] 3535 NM_002284.3(KRT86): c.340A > G −1 — [′GGAGAAGGAGCAGATCAAGTCCCTCVACA (p.Asn114Asp) GCAGGTTCGCGGCCTTCATCGA′] 3536 NM_003865.2(HESX1): c.541A > G 8820 HESX1 [′TCTAATGGCGAAAAAAAATTTCAACRCAA (p.Thr181Ala) ATCTGCTGGAATAGATAGAAAA′] 3537 NM_000303.2(PMM2): c.563A > G 5373 PMM2 [′TTTGATGTCTTTCCTGATGGATGGGRCAAG (p.Asp188Gly) AGATACTGTCTGCGACATGTG′] 3538 NM_000288.3(PEX7): c.340 − 5191 PEX7 [′TCTTTTGCTTTCTAAACACTTTTCARTGTTT 10A > G TTAGGTGTATAGTGTTGATT′] 3539 NM_004044.6(ATIC): c.1277A > G 471 ATIC [′CTCATCGTAGCCACCATTGCTGTCARGTAC (p.Lys426Arg) ACTCAGTCTAACTCTGTGTGC′] 3540 NM_000314.6(PTEN): c.368A > G 5728 PTEN [′GATGACAATCATGTTGCAGCAATTCRCTGT (p.His123Arg) AAAGCTGGAAAGGGACGAACT′] 3541 NM_000314.6(PTEN): c.278A > G 5728 PTEN [′GTTGCACAATATCCTTTTGAAGACCRTAAC (p.His93Arg) CCACCACAGCTAGAACTTATC′] 3542 NM_000314.6(PTEN): c.755A > G 5728 PTEN [′CCTCAGCCGTTACCTGTGTGTGGTGRTATC (p.Asp252Gly) AAAGTAGAGTTCTTCCACAAA′] 3543 NM_018488.2(TBX4): c.1592A > G 9496 TBX4 [′TCCTTGTCCCGAGAATCTTCCTTACRGTAC (p.Gln531Arg) CATTCAGGAATGGGGACTGTG′] 3544 NM_000223.3(KRT12): c.403A > G 3859 KRT12 [′AGAAACTATGCAAAATCTTAATGATRGAT (p.Arg135Gly) TAGCTTCCTACCTGGATAAGGT′] 3545 NM_000503.5(EYA1): c.1639A > G 2138 EYA1 [′TGAGAGAATAATTCAAAGGTTTGGARGAA (p.Arg547Gly) AAGTGGTGTATGTTGTTATAGG′] 3546 NM_000261.1(MYOC): c.1010A > G 4653 MYOC [′GTGTACTCGGGGAGCCTCTATTTCCRGGGC (p.Gln337Arg) GCTGAGTCCAGAACTGTCATA′] 3547 NM_000261.1(MYOC): c.1267A > G 4653 MYOC [′ACAAACCTGGGAGACAAACATCCGTRAGC (p.Lys423Glu) AGTCAGTCGCCAATGCCTTCAT′] 3548 NM_000474.3(TWIST1): c.466A > G 7291 TWIST1 [′GACCCTCAAGCTGGCGGCCAGGTACRTCG (p.Ile156Val) ACTTCCTCTACCAGGTCCTCCA′] 3549 NM_001089.2(ABCA3): c.1702A > 21 ABCA3 [′ACAGATCACCGTCCTGCTGGGCCACRACG G (p.Asn568Asp) GTGCCGGGAAGACCACCACCCT′] 3550 NM_005055.4(RAPSN): c.-210A > G 5913 RAPSN [′ATTCCTCAGAGGCCATGTGGCCCCARCTGG CAGCGACAGCTGCAGACGGGC′] 3551 NM_198217.2(ING1): c.515A > G 3621 ING1 [′AACGAACCCACGTACTGTCTGTGCARCCA (p.Asn172Ser) GGTCTCCTATGGGGAGATGATC′] 3552 NM_000430.3(PAFAH1B1): c.446A > 5048 PAFAH1B1 [′GATTTTGAACGAACTCTTAAAGGACRTAC G (p.His149Arg) AGACTCTGTACAGGACATTTCA′] 3553 NM_000579.3(CCR5): c.-301 + −1 — [′TACGGGGAGAGTGGAGAAAAAGGGGRCA 246A > G CAGGGTTAATGTGAAGTCCAGGA′] 3554 NM_000264.3(PTCH1): c.2479A > G −1 — [′ACTTTACGACCTACACAGGAGTTTCRGTAA (p.Ser827Gly) CGTGAAGTATGTCATGTTGGA′] 3555 NM_001184.3(ATR): c.2022A > G 545 ATR [′TCTGCTGCTGCAATAAGATAAAAAAYCCA (p.Gly674=) CTAACACAACTAGCCCGGATTA′] 3556 NM_005982.3(SIX1): c.386A > G 6495 SIX1 [′ATCTGGGACGGCGAGGAGACCAGCTRCTG (p.Tyr129Cys) CTTCAAGGAGAAGTCGAGGGGT′] 3557 NM_006267.4(RANBP2): c.1966A > 5903 RANBP2 [′AGAAGACGCACACATAACTTTTGCTRTATT G (p.Ile656Val) GGATGCAGTAAATGGAAATAT′] 3558 NM_001001557.2(GDF6): c.1271A > 392255 GDF6 [′CCGCCCAGCTGCTGCGTGCCCACCARATTG > G (p.Lys424Arg) ACTCCCATCAGCATTCTATAC′] 3559 NM_000557.4(GDF5): c.517A > G 8200 GDF5 [′ACCCCCCATCACACCCCACGAGTACVTGCT (p.Met173Val) CTCGCTGTACAGGACGCTGTC′] 3560 NM_000392.4(ABCC2): c.4145A > 1244 ABCC2 [′CGAGAGAAGCTGACCATCATCCCCCRGGT G (p.Gln1382Arg) GAGCTCTAGAACTTACTCGGGC′] 3561 NM_000396.3(CTSK): c.990A > G 1513 CTSK [′ACCTGGCCAGCTTCCCCAAGATGTGRCTCC (p.Ter330Trp) AGCCAGCCAAATCCATCCTGC′] 3562 NM_001127221.1(CACNA1A): c.4151A > 773 CACNA1A [′AACGTCTTCAACATCCTCATCGTCTRCATG G (p.Tyr1384Cys) CTATTCATGTTCATCTTCGCC′] 3563 NM_000178.2(GSS): c.656A > G 2937 GSS [′CAAGAGAAGGAAAGAAACATATTTGRCCA (p.Asp219Gly) GCGTGCCATAGAGAATGAGCTA′] 3564 NM_000901.4(NR3C2): c.2327A > G 4306 NR3C2 [′ACGCTCAACCGCTTAGCAGGCAAACRGAT (p.Gln776Arg) GATCCAAGTCGTGAAGTGGGCA′] 3565 NM_000901.4(NR3C2): c.2915A > G 4306 NR3C2 [′ATCAGCGACCAGCTGCCCAAGGTGGRGTC (p.Glu972Gly) GGGGAACGCCAAGCCGCTCTAC′] 3566 NM_004407.3(DMP1): c.1A > G 1758 DMP1 [′CCAGGTAGAGGTATCACACCCAACTRTGA (p.Met1Val) AGATCAGCATCCTGCTCATGTT′] 3567 NM_004999.3(MYO6): c.737A > G 4646 MYO6 [′CAAGGCAAAGAGGAAAGAAATTATCRTAT (p.His246Arg) CTTTTATAGGTTGTGTGCTGGT′] 3568 NM_000256.3(MYBPC3): c.175A > 4607 MYBPC3 [′CGCCAGCAACAAGTACGGCCTGGCCRCAG G (p.Thr59Ala) AGGGCACACGGCATACGCTGAC′] 3569 NM_000163.4(GHR): c.594A > G 2690 GHR [′TGGAGTATGAACTTCAATACAAAGARGTA (p.Glu198=) AATGAAACTAAATGGAAAATGG′] 3570 NM_000525.3(KCNJ11): c.509A > G 3767 KCNJ11 [′ATCATGCTTGGCTGCATCTTCATGARGACT (p.Lys170Arg) GCCCAAGCCCACCGCAGGGCT′] 3571 NM_000525.3(KCNJ11): c.776A > G 3767 KCNJ11 [′CTGGTGGCCCCGCTGATCATCTACCRTGTC (p.His259Arg) ATTGATGCCAACAGCCCACTC′] 3572 NM_000182.4(HADHA): c.180 + 3A > 3030 HADHA [′GAAAAAAACTGCAAATTAAATGAGAYACC G TTTGAATTGGGAGAGTTAATTC′] 3573 NM_005263.3(GFI1): c.1145A > G 2672 GFI1 [′GGCAAGGCATTCAGCCAGAGCTCCARCCT (p.Asn382Ser) CATCACCCACAGCCGCAAACAC′] 3574 NM_005263.3(GFI1): c.1208A > G 2672 GFI1 [′CCCTTCGGCTGCGACCTCTGTGGGARGGGT (p.Lys403Arg) TTCCAGAGGAAGGTGGACCTC′] 3575 NM_001893.4(CSNK1D): c.130A > 1453 CSNK1D [′TGCCATCAAGCTTGAATGTGTCAAARCCA G (p.Thr44Ala) AACACCCTCAGCTCCACATTGA′] 3576 NM_006204.3(PDE6C): c.1363A > G 5146 PDE6C [′AAACAGAAAGGACATTGCTCAGGAARTGC (p.Met455Val) TCATGAACCAAACCAAAGCCAC′] 3577 NM_006204.3(PDE6C): c.1483 − 5146 PDE6C [′TGAAACAACCCATCCTTATTTCAACRGAAA 2A > G GAGGACTTGCCAGACCCACGC′] 3578 NM_003476.4(CSRP3): c.206A > G 8048 CSRP3 [′TGCTATGGGCGCAGATATGGCCCCARAGG (p.Lys69Arg) GATCGGGTATGGACAAGGCGCT′] 3579 NM_005591.3(MRE11A): c.350A > 4361 MRE11A [′GTGAACTATCAAGATGGCAACCTCARCAT G (p.Asn117Ser) TTCAATTCCAGTGTTTAGTATT′] 3580 NM_000107.2(DDB2): c.730A > G 1643 DDB2 [′TTGGAATCTCAGAATGCACAAAAAGRAAG (p.Lys244Glu) TGACGCATGTGGCCCTGAACCC′] 3581 NM_001204.6(BMPR2): c.1454A > 659 BMPR2 [′AAGGAGACAATCGAAGACTGTTGGGRCCA G (p.Asp485Gly) GGATGCAGAGGCTCGGCTTACT′] 3582 NM_000336.2(SCNN1B): c.863A > 6338 SCNN1B [′ACAGAGAAGGCACTTCCTTCGGCCARCCC G (p.Asn288Ser) TGGAACTGAATTCGGTGAGTTT′] 3583 NM_000447.2(PSEN2): c.715A > G 5664 PSEN2 [′CTACCTCATCATGATCAGTGCGCTCRTGGC (p.Met239Val) CCTAGTGTTCATCAAGTACCT′] 3584 NM_002181.3(IHH): c.284A > G 3549 IHH [′CCAGACATCATCTTCAAGGACGAGGRGAA (p.Glu95Gly) CACAGGCGCCGACCGCCTCATG′] 3585 NM_000310.3(PPT1): c.236A > G 5538 PPT1 [′ACTTCTCTCTGGCTTCTTTTTTAGGRCGTGG (p.Asp79Gly) AGAACAGCTTCTTCTTGAAT′] 3586 NM_003051.3(SLCl6A1): c.610A > 6566 SLC16A1 [′GCGACCAATCGGGCCCAAGCCAACCRAGG G (p.Lys204Glu) CAGGGAAAGATAAGTCTAAAGC′] 3587 NM_005587.2(MEF2A): c.788A > G 4205 MEF2A [′TCTCCCCCTCCACCAGGTGGTGGTARTCTT (p.Asn263Ser) GGAATGAACAGTAGGAAACCA′] 3588 NM_000098.2(CPT2): c.638A > G 1376 CPT2 [′TACCTGGTCAATGCGTATCCCCTGGRTATG (p.Asp213Gly) TCCCAGTATTTTCGGCTTTTC′] 3589 NM_000098.2(CPT2): c.359A > G 1376 CPT2 [′TTTTTAGGACCCTGGTTTGATATGTRCCTA (p.Tyr120Cys) TCTGCTCGAGACTCCGTTGTT′] 3590 NM_001079668.2(NKX2-1): c.464 − 7080 NKX2-1 [′TCGCCGGGCCCATGAAGCGGGAGACHGTA 2A > G AGCGACAAACGCACAGCGTCGG′] 3591 NM_004387.3(NKX2-5): c.896A > G 1482 NKX2-5 [′AACTTCGTGAACTTCGGCGTCGGGGRCTTG (p.Asp299Gly) AATGCGGTTCAGAGCCCCGGG′] 3592 NM_004387.3(NKX2-5): c.547A > G 1482 NKX2-5 [′GCTGAAACTCACGTCCACGCAGGTCRAGA (p.Lys183Glu) TCTGGTTCCAGAACCGGCGCTA′] 3593 NM_178138.4(LHX3): c.332A > G 8022 LHX3 [′GTGCGCCGCGCCCAGGACTTCGTGTRCCAC (p.Tyr111Cys) CTGCACTGCTTTGCCTGCGTC′] 3594 NM_001698.2(AUH): c.263 − 2A > G 549 AUH [′CTTGTACTTTTTTTCCCCTTTAACTRGGAAT TGTGGTGCTTGGAATAAACA′] 3595 NM_001698.2(AUH): c.943 − 2A > G 549 AUH [′ACATATTTAATATTTTGTTTTTCTTRGACCA TTCCAACAAAAGACAGACTT′] 3596 NM_001876.3(CPT1A): c.1361A > G 1374 CPT1A [′ATGTGTGTTTCACGTAGGTGGTTTGRCAAG (p.Asp454Gly) TCGTTCACGTTTGTTGTCTTC′] 3597 NM_001876.3(CPT1A): c.1079A > G 1374 CPT1A [′CGGCTGCTGAAGCCCCGGGAGATGGRGCA (p.Glu360Gly) GCAGATGCAGAGGATCCTGGAC′] 3598 NM_001876.3(CPT1A): c.1493A > G 1374 CPT1A [′TCCATTGACAGCCTCCAGCTGGGCTRTGCG (p.Tyr498Cys) GAGGATGGGCACTGCAAAGGC′] 3599 NM_000352.4(ABCC8): c.4270A > 6833 ABCC8 [′CACCCTGCGCTCACGCCTCTCCATCRTCCT G (p.Ile1424Val) GCAGGACCCCGTCCTCTTCAG′] 3600 NM_000352.4(ABCC8): c.215A > G 6833 ABCC8 [′ACATGGCTTCATTTCCCTGGGCACARCCTG (p.Asn72Ser) CGGTGGATCCTGACCTTCATG′] 3601 NM_002180.2(IGHMBP2): c.638A > 3508 IGHMBP2 [′TCTCAGAAAGAACTTGCCATCATCCRTGGA G (p.His213Arg) CCTCCTGGCACTGGGAAAACC′] 3602 NM_033163.3(FGF8): c.298A > G 2253 FGF8 [′GAAGCACGTGCAGGTCCTGGCCAACRAGC (p.Lys100Glu) GCATCAACGCCATGGCAGAGGA′] 3603 NM_006180.4(NTRK2): c.2165A > 4915 NTRK2 [′TCCCGGGACGTGTACAGCACTGACTRCTAC G (p.Tyr722Cys) AGGGTGAGTAGCTGTGCAGAT′] 3604 NM_033028.4(BBS4): c.157 − 2A > G 585 BBS4 [′TCAGAAGCATTTTTCTCCCTCTTTCRGGCT GTTATCAAAGAACAGCTTCAA′] 3605 NM_000409.3(GUCA1A): c.296A > 2978 GUCA1A [′AAGCTCCGCTGGTACTTCAAGCTCTRTGAT G (p.Tyr99Cys) GTAGATGGCAACGGCTGCATT′] 3606 NM_000344.3(SMN1): c.815A > G 6606 SMN1 [′TTAATTTCATGGTACATGAGTGGCTRTCAT (p.Tyr272Cys) ACTGGCTATTATATGGTAAGT′] 3607 NM_000344.3(SMN1): c.784A > G 6606 SMN1 [′TCTTGATGATGCTGATGCTTTGGGARGTAT (p.Ser262Gly) GTTAATTTCATGGTACATGAG′] 3608 NM_000095.2(COMP): c.1358A > G 1311 COMP [′CGGGACAACTGTCCCACGGTGCCTARCAG (p.Asn453Ser) TGCCCAGGAGGACTCAGACCAC] 3609 NM_000095.2(COMP): c.1418A > G 1311 COMP [′GGTGATGCCTGCGACGACGACGACGRCAA (p.Asp473Gly) TGACGGAGTCCCTGACAGTCGG] 3610 NM_002047.2(GARS): c.374A > G 2617 GARS [′GATATTGTAGACCGAGCAAAAATGGRAGA (p.Glu125Gly) TACCCTGAAGAGGAGGTTTTTC′] 3611 NM_001166107.1(HMGCS2): c.500A > 3158 HMGCS2 [′GGCATAGATACCACCAATGCCTGCTRCGG G (p.Tyr167Cys) TGGTACTGCCTCCCTCTTCAAT′] 3612 NM_000161.2(GCH1): c.671A > G 2643 GCH1 [′CGAGGTGTACAGAAAATGAACAGCARAAC (p.Lys224Arg) TGTGACCAGCACAATGTTGGGT′] 3613 NM_001024630.3(RUNX2): c.598A > 860 RUNX2 [′ATCTGCAGGCAAGAGTTTCACCTTGRCCAT G (p.Thr200Ala) AACCGTCTTCACAAATCCTCC′] 3614 NM_000423.2(KRT2): c.556A > G 3849 KRT2 [′AGAGCGTGAGCAGATCAAAACTCTCDACA (p.Asn186Asp) ACAAATTTGCCTCCTTCATTGA′] 3615 NM_000215.3(JAK3): c.299A > G 3718 JAK3 [′GATGCCAGCACCCAAGTCCTGCTGTRCAG (p.Tyr100Cys) GATTCGGTAGGAAGTGCCCCCC′] 3616 NM_000335.4(SCN5A): c.3971A > G 6331 SCN5A [′CTCTGTCCACTTGAGGTGGTGGTCARTGCC (p.Asn1324Ser) CTGGTGGGCGCCATCCCGTCC′] 3617 NM_000335.4(SCN5A): c.5381A > G 6331 SCN5A [′AGTGAGGACGACTTCGATATGTTCTRTGAG (p.Tyr1794Cys) ATCTGGGAGAAATTTGACCCA] 3618 NM_001014797.2(KCNMA1): c.1301A > 3778 KCNMA1 [′AAGGACTTTCTGCACAAGGACCGGGRTGA G (p.Asp434Gly) CGTCAATGTGGAGATCGTTTTT′] 3619 NM_004380.2(CREBBP): c.3524A > 1387 CREBBP [′TATAATCGCAAGACATCCCGAGTCTRTAA G (p.Tyr1175Cys) GTTTTGCAGTAAGCTTGCAGAG′] 3620 NM_000023.2(SGCA): c.410A > G 6442 SGCA [′GGCCCCCTGCTGCCATACCAAGCCGRGTTC (p.Glu137Gly) CTGGTGCGCAGCCACGATGCG′] 3621 NM_002835.3(PTPN12): c.182A > G 5782 PTPN12 [′GGAGAAAAAGAAGAAAATGTTAAAARGA (p.Lys61Arg) ACAGATACAAGGACATACTGCCA′] 3622 NM_000211.4(ITGB2): c.1052A > G 3689 ITGB2 [′GGGGAGCTGTCTGAGGACTCCAGCARTGT (p.Asn351Ser) GGTCCAACTCATTAAGAATGCT′] 3623 NM_005502.3(ABCA1): c.1790A > 19 ABCA1 [′GTCTGGGGGGGCTTCGCCTACTTGCRGGAT G (p.Gln597Arg) GTGGTGGAGCAGGCAATCATC′] 3624 NM_005502.3(ABCA1): c.2804A > 19 ABCA1 [′CAGATCACCTCCTTCCTGGGCCACARTGGA G (p.Asn935Ser) GCGGGGAAGACGACCACCATG′] 3625 m.5843A > G 4579 MT-TY [′CTCGGAGCTGGTAAAAAGAGGCCTARCCC CTGTCTTTAGATTTACAGTCCA′] 3626 m.7445A > G −1 — [′AAGAACCCGTATACATAAAATCTAGVCAA AAAAGGAAGGAATCGAACCCCC′] 3627 m.8344A > G 4566 MT-TK [′ACCTTTTAAGTTAAAGATTAAGAGARCCA ACACCTCTTTACAGTGAAATGC′] 3628 m.8296A > G 4566 MT-TK [′ACCCCCTCTACCCCCTCTAGAGCCCRCTGT AAAGCTAACTTAGCATTAACC′] 3629 m.12320A > G 4568 MT-TL2 [′TCTTAGGCCCCAAAAATTTTGGTGCRACTC CAAATAAAAGTAATAACCATG′] 3630 m.3243A > G 4567 MT-TL1 [′AGAACAGGGTTTGTTAAGATGGCAGRGCC CGGTAATCGCATAAAACTTAAA′] 3631 m.3252A > G 4567 MT-TL1 [′TTTGTTAAGATGGCAGAGCCCGGTARTCGC ATAAAACTTAAAACTTTACAG′] 3632 m.3251A > G 4567 MT-TL1 [′GTTTGTTAAGATGGCAGAGCCCGGTRATC GCATAAAACTTAAAACTTTACA′] 3633 m.3260A > G 4567 MT-TL1 [′GATGGCAGAGCCCGGTAATCGCATARAAC TTAAAACTTTACAGTCAGAGGT′] 3634 m.3274A > G 4567 MT-TL1 [′GTAATCGCATAAAACTTAAAACTTTRCAGT CAGAGGTTCAATTCCTCTTCT′] 3635 m.4317A > G 4565 MT-TI [′GATAGAGTAAATAATAGGAGCTTAARCCC CCTTATTTCTAGGACTATGAGA′] 3636 m.4269A > G 4565 MT-TI [′GCATTCCCCCTCAAACCTAAGAAATRTGTC TGATAAAAGAGTTACTTTGAT′] 3637 m.4295A > G 4565 MT-TI [′TGTCTGATAAAAGAGTTACTTTGATRGAGT AAATAATAGGAGCTTAAACCC′] 3638 m.4300A > G 4565 MT-TI [′GATAAAAGAGTTACTTTGATAGAGTRAAT AATAGGAGCTTAAACCCCCTTA′] 3639 m.10044A > G 4563 MT-TG [′CTTCCAATTAACTAGTTTTGACAACRTTCA AAAAAGAGTAATAAACTTCGC′] 3640 m.7526A > G 4555 MT-TD [′TCCATGACTTTTTCAAAAAGGTATTRGAAA AACCATTTCATAACTTTGTCA′] 3641 m.10438A > G 4573 MT-TR [′AGTTTAAACAAAACGAATGATTTCGRCTC ATTAAATTATGATAATCATATT′] 3642 m.1555A > G 4549 MT-RNR1 [′ACCCCTACGCATTTATATAGAGGAGRCAA GTCGTAACATGGTAAGTGTACT′] 3643 m.827A > G 4549 MT-RNR1 [′CACCCCCACGGGAAACAGCAGTGATNARC CTTTAGCAATAAACGAAAGTTT′] 3644 m.15579A > G 4519 MT-CYB [′CCCGAATGATATTTCCTATTCGCCTRCACA ATTCTCCGATCCGTCCCTAAC′] 3645 m.14495A > G 4541 MT-ND6 [′TCCAAAGACAACCATCATTCCCCCTRAATA AATTAAAAAAACTATTAAACC′] 3646 m.12770A > G 4540 MT-ND5 [′CTATTCCAACTGTTCATCGGCTGAGRGGGC GTAGGAATTATATCCTTCTTG′] 3647 m.11084A > G 4538 MT-ND4 [′CTCCCTACAAATCTCCTTAATTATARCATT CACAGCCACAGAACTAATCAT′] 3648 m.3397A > G 4535 MT-ND1 [′TACCGAACGAAAAATTCTAGGCTATRTAC AACTACGCAAAGGCCCCAACGT′] 3649 m.4136A > G 4535 MT-ND1 [′CTGTTCTTATGAATTCGAACAGCATRCCCC CGATTCCGCTACGACCAACTC′] 3650 m.3796A > G 4535 MT-ND1 [′CCGTTTACTCAATCCTCTGATCAGGNTGAG - CATCAAACTCAAACTACGCCC′, 3654 ′AATAAGTGGCTCCTTTAACCTCTCCDCCCTT ATCACAACACAAGAACACCT′, ′ACCCCCTTGACCTTGCCGAAGGGGANTCCG AACTAGTCTCAGGCTTCAACA′, ′CGAATACGCCGCAGGCCCCTTCGCCNTATT CTTCATAGCCGAATACACAAA′, ′ATTTTGTCACCAAGACCCTACTTCTNACCTC CCTGTTCTTATGAATTCGAA′] 3655 NM_003334.3(UBA1): c.1639A > G 7317 UBA1 [′AATGAATCCACATATCCGGGTGACARGCC (p.Ser547Gly) ACCAGAACCGTGTGGGTCCTGA′] 3656 NM_000354.5(SERPINA7): c.623 − 6906 SERPINA7 [′GATCAAAAGGATTTGCCCACTGGGCYTAA 2A > G AATACAAAGAAAAGAGAGGTGT′] 3657 NM_000044.3(AR): c.2291A > G 367 AR [′ACCAATGTCAACTCCAGGATGCTCTRCTTC (p.Tyr764Cys) GCCCCTGATCTGGTTTTCAAT′] 3658 NM_000044.3(AR): c.2362A > G 367 AR [′CCGGATGTACAGCCAGTGTGTCCGARTGA (p.Met788Val) GGCACCTCTCTCAAGAGTTTGG′] 3659 NM_000044.3(AR): c.2632A > G 367 AR [′GATTGCGAGAGAGCTGCATCAGTTCRCTTT (p.Thr878Ala) TGACCTGCTAATCAAGTCACA′] 3660 NM_000044.3(AR): c.2708A > G 367 AR [′ATGATGGCAGAGATCATCTCTGTGCRAGT (p.Gln903Arg) GCCCAAGATCCTTTCTGGGAAA′] 3661 NM_002764.3(PRPS1): c.341A > G 5631 PRPS1 [′CCAATCTCAGCCAAGCTTGTTGCAARTATG (p.Asn114Ser) CTATCTGTAGCAGGTGCAGAT′] 3662 NM_000194.2(HPRT1): c.602A > G 3251 HPRT1 [′CTTGACTATAATGAATACTTCAGGGRTTTG (p.Asp201Gly) AATGTAAGTAATTGCTTCTTT′] 3663 NM_000194.2(HPRT1): c.155A > G 3251 HPRT1 [′TGTAGGACTGAACGTCTTGCTCGAGRTGTG (p.Asp52Gly) ATGAAGGAGATGGGAGGCCAT′] 3664 NM_000132.3(F8): c.872A > G 2157 F8 [′CCTGAAGTGCACTCAATATTCCTCGRAGGT (p.Glu291Gly) CACACATTTCTTGTGAGGAAC′] 3665 NM_000132.3(F8): c.5183A > G 2157 F8 [′GCTGCAGTGGAGAGGCTCTGGGATTRTGG (p.Tyr1728Cys) GATGAGTAGCTCCCCACATGTT′] 3666 NM_000132.3(F8): c.5821A > G 2157 F8 [′TTCTCTGTGTCCTTCTCCAGCAATCRATGG (p.Asn1941Asp) CTACATAATGGATACACTACC] 3667 NM_000132.3(F8): c.328A > G 2157 F8 [′TACAGTGGTCATTACACTTAAGAACRTGGC (p.Met110Val) TTCCCATCCTGTCAGTCTTCA′] 3668 NM_000132.3(F8): c.398A > G 2157 F8 [′TTTCTTCCTGCTATAGGAGCTGAATRTGAT (p.Tyr133Cys) GATCAGACCAGTCAAAGGGAG′] 3669 NM_000132.3(F8): c.404A > G 2157 F8 [′CCTGCTATAGGAGCTGAATATGATGRTCA (p.Asp135Gly) GACCAGTCAAAGGGAGAAAGAA′] 3670 NM_000132.3(F8): c.940A > G 2157 F8 [′GGAAATCTCGCCAATAACTTTCCTTRCTGC (p.Thr314Ala) TCAAACACTCTTGATGGACCT′] 3671 NM_000132.3(F8): c.1226A > G 2157 F8 [′TGGGTACATTACATTGCTGCTGAAGRGGA (p.Glu409Gly) GGACTGGGACTATGCTCCCTTA′] 3672 NM_000132.3(F8): c.1331A > G 2157 F8 [′CAGCGGATTGGTAGGAAGTACAAAARAGT (p.Lys444Arg) CCGATTTATGGCATACACAGAT′] 3673 NM_000132.3(F8): c.1418A > G 2157 F8 [′TCAGGAATCTTGGGACCTTTACTTTRTGGG (p.Tyr473Cys) GAAGTTGGAGACACACTGTTG′] 3674 NM_000132.3(F8): c.1660A > G 2157 F8 [′TCGGTGCCTGACCCGCTATTACTCTRGTTT (p.Ser554Gly) CGTTAATATGGAGAGAGATCT′] 3675 NM_000132.3(F8): c.1682A > G 2157 F8 [′TCTAGTTTCGTTAATATGGAGAGAGRTCTA (p.Asp561Gly) GCTTCAGGACTCATTGGCCCT′] 3676 NM_000132.3(F8): c.1892A > G 2157 F8 [′GAGGATCCAGAGTTCCAAGCCTCCARCAT (p.Asn631Ser) CATGCACAGTGAGTAAAGCAGC′] 3677 NM_000132.3(F8): c.5600A > G 2157 F8 [′TTCCCTCCCTAGGAAAAAGATGTGCRCTCA (p.His1867Arg) GGCCTGATTGGACCCCTTCTG′] 3678 NM_000132.3(F8): c.5822A > G 2157 F8 [′TCTCTGTGTCCTTCTCCAGCAATCARTGGC (p.Asn1941Ser) TACATAATGGATACACTACCT′] 3679 NM_000132.3(F8): c.6113A > G 2157 F8 [′AGCACACTTTTTCTGGTGTACAGCARTAGT (p.Asn2038Ser) GAGTAGCAATGTGGGCAGAGG′] 3680 NM_000132.3(F8): c.6278A > G 2157 F8 [′AAGTGTTATTTTAATTGGTAGGTGGRTCTG (p.Asp2093Gly) TTGGCACCAATGATTATTCAC′] 3681 NM_000132.3(F8): c.6371A > G 2157 F8 [′TACATCTCTCAGTTTATCATCATGTRTAGT CTTGATGGGAAGAAGTGGCAG′] 3682 NM_000132.3(F8): c.6794A > G 2157 F8 [′ATGAAAGTCACAGGAGTAACTACTCRGGG (p.Gln2265Arg) AGTAAAATCTCTGCTTACCAGC′] 3683 NM_000132.3(F8): c.104A > G 2157 F8 [′GGTGCAGTGGAACTGTCATGGGACTRTAT (p.Tyr35Cys) GCAAAGTGATCTCGGTGAGCTG′] 3684 NM_000402.4(G6PD): c.466A > G 2539 G6PD [′CAACTCCTATGTGGCTGGCCAGTACRATGA (p.Asn156Asp) TGCAGCCTCCTACCAGCGCCT′] 3685 NM_000402.4(G6PD): c.583A > G 2539 G6PD [′GCTCCGGGCTCCCAGCAGAGGCTGGRACC (p.Asn195Asp) GCATCATCGTGGAGAAGCCCTT′] 3686 NM_002049.3(GATA1): c.653A > G 2623 GATA1 [′ACAGCCACTCCACTGTGGCGGAGGGRCAG (p.Asp218Gly) GACAGGCCACTACCTATGCAAC′] 3687 NM_001097642.2(GJB1): c.194A > 2705 GJB1 [′CAGCCTGGCTGCAACAGCGTTTGCTRTGAC G (p.Tyr65Cys) CAATTCTTCCCCATCTCCCAT′] 3688 NM_000166.5(GJB1): c.614A > G 2705 GJB1 [′GCCTCTGGCATCTGCATCATCCTCARTGTG (p.Asn205Ser) GCCGAGGTGGTGTACCTCATC′] 3689 NM_000032.4(ALAS2): c.1702A > G 212 ALAS2 [′CCGTCCTGTACACTTTGAGCTCATGRGTGA (p.Ser568Gly) GTGGGAACGTTCCTACTTCGG′] 3690 NM_000202.6(IDS): c.404A > G 3423 IDS [′GGCTATGTGACCATGTCGGTGGGAARAGT (p.Lys135Arg) CTTTCACCCTGGTACTGCTCCA′] 3691 NM_000292.2(PHKA2): c.896A > G 5256 PHKA2 [′TATGGATGCTGTCGCTTCCTTCGAGRTGGT (p.Asp299Gly) TATAAAACTCCAAGAGAGGTT′] 3692 NM_000292.2(PHKA2): c.565A > G 5256 PHKA2 [′TTATGGAATGTGGGAGCGTGGAGATRAGA (p.Lys189Glu) CTAATCAGGGCATCCCGGAATT′] 3693 NM_000351.4(STS): c.1331A > G 412 STS [′CGCTCCGATCATGAGTTTCTCTTCCRTTAC (p.His444Arg) TGCAACGCCTACTTAAATGCT′] 3694 NM_000133.3(F9): c.278A > G 2158 F9 [′TTCAATTTCTTAACCTATCTCAAAGRTGGA (p.Asp93Gly) GATCAGTGTGAGTCCAATCCA′] 3695 NM_000133.3(F9): c.329A > G 2158 F9 [′TGTTTAAATGGCGGCAGTTGCAAGGRTGA (p.Asp110Gly) CATTAATTCCTATGAATGTTGG′] 3696 NM_000133.3(F9): c.917A > G 2158 F9 [′CGAATTATTCCTCACCACAACTACARTGCA (p.Asn306Ser) GCTATTAATAAGTACAACCAT′] 3697 NM_000133.3(F9): c.1180A > G 2158 F9 [′TACAAAGTTCACCATCTATAACAACRTGTT (p.Met394Val) CTGTGCTGGCTTCCATGAAGG′] 3698 NM_000133.3(F9): c.1231A > G 2158 F9 [′AGGTAGAGATTCATGTCAAGGAGATRGTG (p.Ser411Gly) GGGGACCCCATGTTACTGAAGT′] 3699 NM_000266.3(NDP): c.131A > G 4693 NDP [′CCTCGACGCTGCATGAGGCACCACTRTGTG (p.Tyr44Cys) GATTCTATCAGTCACCCATTG′] 3700 NM_000266.3(NDP): c.1A > G 4693 NDP [′TCTAGAGAAGTTTTTCCTTACAACARTGAG (p.Met1Val) AAAACATGTACTAGCTGCATC′] 3701 NM_000266.3(NDP): c.125A > G 4693 NDP [′TCGGACCCTCGACGCTGCATGAGGCRCCA (p.His42Arg) CTATGTGGATTCTATCAGTCAC′] 3702 NM_000169.2(GLA): c.886A > G −1 — [′GGCTATCATGGCTGCTCCTTTATTCRTGTC (p.Met296Val) TAATGACCTCCGACACATCAG′] 3703 NM_000169.2(GLA): c.101A > G −1 — [′ATCCCTGGGGCTAGAGCACTGGACARTGG (p.Asn34Ser) ATTGGCAAGGACGCCTACCATG′] 3704 NM_000169.2(GLA): c.644A > G −1 — [′GAGTCATATCTGTTTTCACAGCCCARTTAT (p.Asn215Ser) ACAGAAATCCGACAGTACTGC′] 3705 NM_000169.2(GLA): c.1228A > G −1 — [′AAGGTTAAGAAGTCACATAAATCCCRCAG (p.Thr410Ala) GCACTGTTTTGCTTCAGCTAGA′] 3706 NM_000169.2(GLA): c.815A > G −1 — [′TCTTTTTCTCAGTTAGTGATTGGCARCTTTG (p.Asn272Ser) GCCTCAGCTGGAATCAGCAA′] 3707 NM_153252.4(BRWD3): c.4786A > 254065 BRWD3 [′AGAAGATAAAGAGAAAAAAGAAACARAA G (p.Lys1596Glu) GAGAAATCTCATTTATCCACCTC′] 3708 NM_004463.2(FGD1): c.1396A > G 2245 FGD1 [′GCAGAAACTGGCCCCCTTCCTCAAGRTGTA (p.Met466Val) TGGTGAGTATGTGAAGAACTT′] 3709 NM_000054.4(AVPR2): c.614A > G 554 AVPR2 [′GCGGAGCCCTGGGGCCGTCGCACCTRTGT (p.Tyr205Cys) CACCTGGATTGCCCTGATGGTG′] 3710 NM_000054.4(AVPR2): c.839A > G 554 AVPR2 [′ATGACGCTAGTGATTGTGGTCGTCTRTGTG (p.Tyr280Cys) CTGTGCTGGGCACCCTTCTTC′] 3711 NM_000276.3(OCRL): c.1436A > G 4952 OCRL [′ATCAAGTTCATCCCCACTTATAAGTRTGAC (p.Tyr479Cys) TCTAAAACAGACCGGTGGGAT] 3712 NM_000397.3(CYBB): c.302A > G 1536 CYBB [′CAACTGGACAGGAATCTCACCTTTCRTAAA (p.His101Arg) ATGGTGGCATGGATGATTGCA′] 3713 NM_000397.3(CYBB): c.1499A > G 1536 CYBB [′GCTGTGCACCATGATGAGGAGAAAGRTGT (p.Asp500Gly) GATCACAGGCCTGAAACAAAAG′] 3714 NM_001205019.1(GK): c.880A > G 2710 GK [′TGGAACAGGATGTTTCTTACTATGTRATAC (p.Asn294Asp) AGGCCATAAGGTTGGTTTTTT′] 3715 NM_005710.2(PQBP1): c.194A > G 10084 PQBP1 [′CGGCCCCACAGCGGGCTCCCTTACTRCTGG (p.Tyr65Cys) AATGCAGACACAGACCTTGTA′] 3716 NM_198270.3(NHS): c.853  − 2A > G 4810 NHS [′CTGAACCTGATTGTACTTTGTTTGCRGTCC CATCCCCCAGAGGATGAAGAT′] 3717 NM_000252.2(MTM1): c.566A > G 4534 MTM1 [′CACCATTGGAGAATAACTTTTATTARTAAG (p.Asn189Ser) TGCTATGAGCTCTGTGACACT′] 3718 NM_000252.2(MTM1): c.1190A > G 4534 MTM1 [′GCCATGCTGATGTTGGATAGCTTCTRTAGG (p.Tyr397Cys) AGCATTGAAGGGTTCGAAATA′] 3719 NM_000252.2(MTM1): c.1261 − 4534 MTM1 [′TAATTAAAACAAATTATCTTCATCARTTTA 10A > G TTCAGCGAATAGGTCATGGTG′] 3720 NM_001015877.1(PHF6): c.700A > 84295 PHF6 [′AGGAAAACTGCATATATTTAATGCCRAGA G (p.Lys234Glu) AGGCAGCTGCCCATTATAAGTG′] 3721 NM_001015877.1(PHF6): c.686A > 84295 PHF6 [′GAAAATGAAGCACGAGGAAAACTGCRTAT G (p.His229Arg) ATTTAATGCCAAGAAGGCAGCT′] 3722 NM_001015877.1(PHF6): c.769A > 84295 PHF6 [′CACAGTCCAGCTCACAACAACATCARGAG G (p.Arg257Gly) CAGAATTTGGAGACTTTGATAT′] 3723 NM_000117.2(EMD): c.1A > G 2010 EMD [′GCCTCCGCCTGAGCCCGCACCCGCCRTGG (p.Met1Val) ACAACTACGCAGATCTTTCGGA′] 3724 NM_004006.2(DMD): c.2317A > G 1756 DMD [′GGCCATAGAGCGAGAAAAAGCTGAGRAGT (p.Lys773Glu) TCAGAAAACTGCAAGATGCCAG′] 3725 NM_004006.2(DMD): c.8734A > G 1756 DMD [′TCTACGAAAGCAGGCTGAGGAGGTCRATA (p.Asn2912Asp) CTGAGTGGGAAAAATTGAACCT′] 3726 NM_004006.2(DMD): c.8762A > G 1756 DMD [′ACTGAGTGGGAAAAATTGAACCTGCRCTC (p.His2921Arg) CGCTGACTGGCAGAGAAAAATA′] 3727 NM_004006.2(DMD): c.835A > G 1756 DMD [′TCCCCCAAACCCTTCTCTGCAGATCRCGGT (p.Thr279Ala) CAGTCTAGCACAGGGATATGA′] 3728 NM_004006.2(DMD): c.9225 − 1756 DMD [′TCGATCGCACTTCAGTTATGATAAAYTGAC 285A > G CTTGTTATGTGATCAATAATC′] 3729 NM_000033.3(ABCD1): c.443A > G 215 ABCD1 [′ATCGCCCTCCCTGCTACCTTCGTCARCAGT (p.Asn148Ser) GCCATCCGTTACCTGGAGGGC′] 3730 NM_003588.3(CUL4B): c.901 − 8450 CUL4B [′TTTGATTTCTTTTTTTTTCATTGGCRGATCA 2A > G TGATCAGGAGCATTTTTTTG′] 3731 NM_000061.2(BTK): c.1288A > G 695 BTK [′GAGAGGCCAGTACGACGTGGCCATCRAGA (p.Lys430Glu) TGATCAAAGAAGGCTCCATGTC′] 3732 NM_000061.2(BTK): c.1082A > G 695 BTK [′AGCACCATCCCTGAGCTCATTAACTRCCAT (p.Tyr361Cys) CAGCACAACTCTGCAGGTGAG′] 3733 NM_000061.2(BTK): c.919A > G 695 BTK [′GGGGAAAGAAGGAGGTTTCATTGTCRGAG (p.Arg307Gly) ACTCCAGCAAAGCTGGCAAATA′] 3734 NM_000061.2(BTK): c.1766A > G 695 BTK [′CACCTTCTAGGGGTTTTGATGTGGGRAATT (p.Glu589Gly) TACTCCCTGGGGAAGATGCCA′] 3735 NM_003413.3(ZIC3): c.1213A > G 7547 ZIC3 [′CTACACGCACCCGAGCTCCCTGCGCRAAC (p.Lys405Glu) ACATGAAGGTAATTACCTCTTT′] 3736 NM_001037811.2(HSD17B10): c.71  3028 HSD17B10 [′GCCATCATCGAGAACCCATTCCTCARTGGA 3A > G (p.Asn238Ser) GAGGTCATCCGGCTGGATGGG′] 3737 NM_003639.4(IKBKG): c.1219A > G 8517 IKBKG [′CAAGTGCCAGTATCAGGCCCCTGATRTGG (p.Met407Val) ACACCCTGCAGATACATGTCAT′] 3738 NM_003639.4(IKBKG): c.1259A > G 8517 IKBKG [′ATACATGTCATGGAGTGCATTGAGTRGGG (p.Ter420Trp) CCGGCCAGTGCAAGGCCACTGC′] 3739 NM_005448.2(BMP15): c.704A > G 9210 BMP15 [′TTGGACATTGCCTTCTTGTTACTCTRTTTCA (p.Tyr235Cys) ATGATACTCATAAAAGCATT′] 3740 NM_005120.2(MED12): c.3020A > 9968 MED12 [′AAGGTGAAGAACACCATCTACTGCARCGT G (p.Asn1007Ser) GGAGCCATCGGAATCAAATATG′] 3741 NM_003688.3(CASK): c.2129A > G 8573 CASK [′GGCAAGAAAAAGAAGCAGTACAAAGRTA (p.Asp710Gly) AATATTTGGCAAAGCACAATGCA′] 3742 NM_001363.4(DKC1): c.361A > G 1736 DKC1 [′ACTTCGGGTGGAGAAGACAGGGCACRGTG (p.Ser121Gly) GTACTCTGGATCCCAAGGTGAC′] 3743 NM_001363.4(DKC1): c.1069A > G 1736 DKC1 [′ATTAATGACCACAGCGGTCATCTCTRCCTG (p.Thr357Ala) CGACCATGGTATAGTAGCCAA′] 3744 NM_000307.4(POU3F4): c.1000A > 5456 POU3F4 [′GTTCTGTAATCGAAGACAAAAAGAGRAAA G (p.Lys334Glu) GAATGACTCCGCCAGGGGATCA′] 3745 NM_004429.4(EFNB1): c.472A > G 1947 EFNB1 [′GGGCGGTGTGTGCCGCACACGCACCRTGA (p.Met158Val) AGATCATCATGAAGGTTGGGCA′] 3746 NM_000489.4(ATRX): c.4826A > G 546 ATRX [′TTCTTATAGGTGGTAAGTTTTCTTCRTACA (p.His1609Arg) GTTCTTTTGTGTGACAAACTG′] 3747 NM_000489.4(ATRX): c.5579A > G 546 ATRX [′TTCTTTTGATCAGGTGTGGGCAATAVTAGT (p.Asn1860Ser) GAAGGTGGAAGAGGAAAGGCA′] 3748 NM_000489.4(ATRX): c.6488A > G 546 ATRX [′GGACAAACTAAGCCTGTTTATGTATRTAGG (p.Tyr2163Cys) TTCTTAGCTCAGGTAGGTTTA] 3749 NM_000489.4(ATRX): c.6811A > G 546 ATRX [′AGAAGAAGAGTTGACTGAAGAAGAARGA (p.Arg2271Gly) AAAGCAGCTTGGGCTGAGTATGA′] 3750 NM_000052.6(ATP7A): c.3911A > G 538 ATP7A [′GTAGCAATGGTGGGAGATGGAATCARTGA (p.Asn1304Ser) CTCCCCAGCTCTGGCAATGGCT′] 3751 NM_004992.3(MECP2): c.410A > G 4204 MECP2 [′GGAAAAGCCTTTCGCTCTAAAGTGGRGTT (p.Glu137Gly) GATTGCGTACTTCGAAAAGGTA′] 3752 NM_000137.2(FAH): c.1141A > G 2184 FAH [′CATAGACCTGGGGAATGGTCAGACCRGGA (p.Arg381Gly) AGTTTCTGCTGGACGGGGATGA′] 3753 NM_000137.2(FAH): c.836A > G 2184 FAH [′CCCTTTGCTGTGCCCAACCCGAAGCRGGTA (p.Gln279Arg) AGCACATTCTCTGCAGGAAGC′] 3754 NM_002769.4(PRSS1): c.68A > G −1 — [′GCTGCCCCCTTTGATGATGATGACARGATC (p.Lys23Arg) GTTGGGGGCTACAACTGTGAG′] 3755 NM_002769.4(PRSS1): c.161A > G −1 — [′CACTTCTGTGGTGGCTCCCTCATCARCGAA (p.Asn54Ser) CAGTGGGTGGTATCAGCAGGC′] 3756 NM_000373.3(UMPS): c.286A > G 7372 UMPS [′AACCAATCAAATTCCAATGCTTATTRGAAG (p.Arg96Gly) GAAAGAAACAAAGGATTATGG′] 3757 NM_001918.3(DBT): c.1355A > G 1629 DBT [′ATGAATGTGAGCTGGTCAGCTGATCRCAG (p.His452Arg) AGTTATTGATGGTGCTACAATG′] 3758 NM_000108.4(DLD): c.214A > G 1738 DLD [′TTGGTTGTAGACAGTCTGCATTGAGRAAA (p.Lys72Glu) ATGAAACACTTGGTGGAACATG′] 3759 NM_000108.4(DLD): c.1483A > G 1738 DLD [′CTTGCAGACCTTATCAGAAGCTTTTRGAGA (p.Arg495Gly) AGCAAATCTTGCTGCGTCATT′] 3760 NM_000108.4(DLD): c.1081A > G 1738 DLD [′CATTGGTGATGTAGTTGCTGGTCCARTGCT (p.Met361Val) GGCTCACAAAGCAGAGGATGA′] 3761 NM_000481.3(AMT): c.125A > G 275 AMT [′CGCAGGACACCGCTCTATGACTTCCRCCTG (p.His42Arg) GCCCACGGCGGGAAAATGGTG′] 3762 NM_000151.3(G6PC): c.230 + 4A > G 2538 G6PC [′GGCTCAACCTCGTCTTTAAGTGGTARGAAC CATATAGAGAGGAGATCAGCA′] 3763 NM_000532.4(PCCB): c.1304A > G 5096 PCCB [′CAGCATTTGGATCTGTTTTAGGCCTRTGGA (p.Tyr435Cys) GGTGCCTATGATGTCATGAGC′] 3764 NM_000387.5(SLC25A20): c.713A > 788 SLC25A20 [′CCAGATGTGCTCAAGTCTCGATTCCRGACT G (p.Gln238Arg) GGTGAGTGGAAGGTAGTGGGG′] 3765 NM_004360.3(CDH1): c.2512A > G 999 CDH1 [′TCTGCTCGTGTTTGACTATGAAGGARGCGG (p.Ser838Gly) TTCCGAAGCTGCTAGTCTGAG′] 3766 NM_004360.3(CDH1): c.1018A > G 999 CDH1 [′GTCGATCTCTCTGCAGAGTTTCCCTDCGTA (p.Thr340Ala) TACCCTGGTGGTTCAAGCTGC′] 3767 NM_003361.3(UMOD): c.383A > G 7369 UMOD [′TGCCACGCCCTGGCCACATGTGTCARTGTG (p.Asn128Ser) GTGGGCAGCTACTTGTGCGTA′] 3768 NM_000463.2(UGT1A1): c.992A > −1 — [′GCTGATGCTTTGGGCAAAATCCCTCRGACA G (p.Gln331Arg) GTAAGAAGATTCTATACCATG′] 3769 NM_000463.2(UGT1A1): c.1085 − −1 — [′TGACATCCTCCCTATTTTGCATCTCRGGTC 2A > G ACCCGATGACCCGTGCCTTTA′] 3770 NM_000463.2(UGT1A1): c.1070A > −1 — [′ACGATACTTGTTAAGTGGCTACCCCRAAAC G (p.Gln357Arg) GATCTGCTTGGTATGTTGGGC′] 3771 NM_000463.2(UGT1A1): c.1198A > −1 — [′GCCCTTGTTTGGTGATCAGATGGACVATGC G (p.Asn400Asp) AAAGCGCATGGAGACTAAGGG′] 3772 NM_001382.3(DPAGT1): c.509A > 1798 DPAGT1 [′TCTCTCCCCGCAGGAATCCTGTACTRTGTC G (p.Tyr170Cys) TACATGGGGCTGCTGGCAGTG′] 3773 NM_001007792.1(NTRK1): c.986A > 4914 NTRK1 [′CCCACCCACGTCAACAACGGCAACTRCAC G (p.Tyr329Cys) GCTGCTGGCTGCCAACCCCTTC′] 3774 NM_001007792.1(NTRK1): c.1651A > 4914 NTRK1 [′CTGCACCGAGGGCCGCCCCCTGCTCRTGGT G (p.Met551Val) CTTTGAGTATATGCGGCACGG′] 3775 NM_000363.4(TNNI3): c.569A > G 7137 TNNI3 [′CTCCAGGAAAACCGGGAGGTGGGAGRCTG (p.Asp190Gly) GCGCAAGAACATCGATGCACTG′] 3776 NM_000363.4(TNNI3): c.532A > G 7137 TNNI3 [′GCGGGCCCACCTCAAGCAGGTGAAGRAGG (p.Lys178Glu) AGGACACCGAGAAGGTGAGTGT′] 3777 NM_001018005.1(TPM1): c.539A > 7168 TPM1 [′ATTGAGAGCGACCTGGAACGTGCAGDGGA G (p.Glu180Gly) GCGGGCTGAGCTCTCAGAAGGG′] 3778 NM_001159287.1(TPI1): c.622A > G 7167 TPI1 [′CCTGGCCTATGAGCCTGTGTGGGCCRTTGG (p.Ile208Val) TACTGGCAAGACTGCAACACC′] 3779 NM_000359.2(TGM1): c.1469A > G 7051 TGM1 [′AATGGCCTGGTCTACATGAAGTACGRCAC (p.Asp490Gly) GCCTTTCATTTTTGCTGAGGTG′] 3780 NM_001024847.2(TGFBR2): c.1472 − 7048 TGFBR2 [′CCCTGTGTTTGCTGGCTTTCTTCACRGAAG 2A > G TAAAAGATTATGAGCCTCCAT′] 3781 NM_003242.5(TGFBR2): c.1273A > 7048 TGFBR2 [′GCTATAGGTGGGAACTGCAAGATACRTGG G (p.Met425Val) CTCCAGAAGTCCTAGAATCCAG′] 3782 NM_004612.3(TGFBR1): c.1199A > 7046 TGFBR1 [′CATTTTGAATCCTTCAAACGTGCTGRCATC G (p.Asp400Gly) TATGCAATGGGCTTAGTATTC′] 3783 NM_001128177.1(THRB): c.1324A > 7068 THRB [′CTGCCATGCCAGCCGCTTCCTGCACRTGAA G (p.Met442Val) GGTGGAATGCCCCACAGAACT′] 3784 NM_001128177.1(THRB): c.1327A 7068 THRB [′CCATGCCAGCCGCTTCCTGCACATGRAGGT > G (p.Lys443Glu) GGAATGCCCCACAGAACTCTT′] 3785 NM_001128177.1(THRB): c.1009A 7068 THRB [′AACCTTGAATGGGGAAATGGCAGTGRCAC > G (p.Thr337Ala) GGGGCCAGCTGAAAAATGGGGG′] 3786 NM_033360.3(KRAS): c.13A > G 3845 KRAS [′AGGCCTGCTGAAAATGACTGAATATRAAC (p.Lys5Glu) TTGTGGTAGTTGGAGCTGGTGG′] 3787 NM_005343.2(HRAS): c.350A > G −1 — [′GTGCCCATGGTGCTGGTGGGGAACARGTG (p.Lys117Arg) TGACCTGGCTGCACGCACTGTG′] 3788 NM_001063.3(TF): c.956A > G 7018 TF [′GACCTGCTGTTTAAGGACTCTGCCCRCGGG (p.His319Arg) TTTTTAAAAGTCCCCCCCAGG′] 3789 NM_001063.3(TF): c.1936A > G 7018 TF [′CTTTTGTTTGTTCCGGTCGGAAACCRAGGA (p.Lys646Glu) CCTTCTGTTCAGAGATGACAC′] 3790 NM_212472.2(PRKAR1A): c.891 + 3A > 5573 PRKAR1A [′ATGAGTTCTTCATTATTTTAGAGGTRAAGA  G ACTCAGAATTTAATACTTGAA′] 3791 NM_212472.2(PRKAR1A): c.1 A > G 5573 PRKAR1A [′GTGTGTTTTTTTCTCGCAGAGAACCRTGGA (p.Met1Val) GTCTGGCAGTACCGCCGCCAG′] 3792 NM_000362.4(TIMP3): c.572A > G −1 — [′GCCTGCATCCGGCAGAAGGGCGGCTRCTG (p.Tyr191Cys) CAGCTGGTACCGAGGATGGGCC′] 3793 NM_000367.3(TPMT): c.719A > G 7172 TPMT [′GATTACAGGTGTGAGCCACCGCACCNAGC - (p.Tyr240Cys) CAATTTTGAGTATTTTTAAAAG′, 3795 ′TAACATGTTACTCTTTCTTGTTTCARGTAAA ATATGCAATATACRTYGTCT′, ′AGGTTGATGCTTTTGAAGAACGACAKAAAA GTTGGGGAATTGACTGTCTTT′] 3796 NM_198253.2(TERT): c.2315A > G 7015 TERT [′TCTACCTTGACAGACCTCCAGCCGTRCATG (p.Tyr772Cys) CGACAGTTCGTGGCTCACCTG′] 3797 NM_000073.2(CD3G): c.1A > G 917 CD3G [′CTTTTGCCGGAGGACAGAGACTGACRTGG (p.MetlVal) AACAGGGGAAGGGCCTGGCTGT′] 3798 NM_000899.4(KITLG): c.107A > G 4254 KITLG [′ATCTGCAGGAATCGTGTGACTAATARTGTA (p.Asn36Ser) AAAGACGTCACTAAATTGGTA′] 3799 NM_001024858.2(SPTB): c.1A > G 6710 SPTB [′GCGGAGCTGCTAAGAGCCTGCTGACRTGA (p.MetlVal) CATCGGCCACAGAGTTTGAAAA′] 3800 NM_003126.2(SPTA1): c.143A > G 6708 SPTA1 [′GAGCGGGTCGCTGAGAGGGGTCAGARGCT (p.Lys48Arg) TGAGGATTCCTATCACTTACAA′] 3801 NM_005633.3(SOS1): c.1654A > G 6654 SOS1 [′TTTACAGTACCGGAGTACACTGGAARGGA (p.Arg552Gly) TGCTTGATGTAACAATGCTACA′] 3802 NM_003041.3(SLC5A2): c.1961A > −1 — [′GACCCGAGCTGGGCCCGTGTGGTCARCCT G (p.Asn654Ser) CAATGCCCTGCTCATGATGGCA′] 3803 NM_000343.3(SLC5A1): c.83A > G 6523 SLC5A1 [′CACGAGCTCATTCGCAATGCAGCCGRTATC (p.Asp28Gly) TCCATCATCGTTATCTACTTC′] 3804 NM_000702.3(ATP1A2): c.1033A > 477 ATP1A2 [′TCTCTACCAGGTGTGCCTGACCCTGRCAGC G (p.Thr345Ala) CAAGCGCATGGCACGGAAGAA′] 3805 NM_003124.4(SPR): c.448A > G 6697 SPR [′CTTCCCGGACAGTCCTGGCCTCAACRGAAC (p.Arg150Gly) CGTGGTTAACATCTCGTCCCT′] 3806 NM_001161766.1(AHCY): c.344A > 191 AHCY [′CTCACCAACCTCATCCACACCAAGTRCCCG G (p.Tyr115Cys) CAGCTTCTGCCAGGTGAGCAG′] 3807 NM_001035.2(RYR2): c.12602A > G 6262 RYR2 [′TGCGAGGACACCATCTTTGAAATGCRGCT (p.Gln4201Arg) GGCGGCTCAGATCTCGGAGTCG′] 3808 NM_000540.2(RYR1): c.14387A > G 6261 RYR1 [′CAGTCCTTCCTGTACCTGGGCTGGTRTATG (p.Tyr4796Cys) GTGATGTCCCTCTTGGGACAC′] 3809 NM_000540.2(RYR1): c.14647 − 6261 RYR1 [′CTCTGTCTCAAAAAAAAAAAAAAACRTAT 1449A > G GTAAAGTTGTTCCCAAATGCCA′] 3810 NM_000540.2(RYR1): c.13909A > G 6261 RYR1 [′GGTGTACTACTTCCTGGAGGAAAGCRCAG (p.Thr4637Ala) GCTACATGGAACCCGCCCTGCG′] 3811 NM_000539.3(RHO): c.533A > G 6010 RHO [′TCTCCCTACCTGCCTGTCCTCAGGTRCATC (p.Tyr178Cys) CCCGAGGGCCTGCAGTGCTCG′] 3812 NM_000539.3(RHO): c.569A > G 6010 RHO [′GGCCTGCAGTGCTCGTGTGGAATCGRCTAC (p.Asp190Gly) TACACGCTCAAGCCGGAGGTC′] 3813 NM_000539.3(RHO): c.886A > G 6010 RHO [′CATGACCATCCCAGCGTTCTTTGCCRAGAG (p.Lys296Glu) CGCCGCCATCTACAACCCTGT′] 3814 NM_000539.3(RHO): c.44A > G 6010 RHO [′CCTAACTTCTACGTGCCCTTCTCCARTGCG (p.Asn15Ser) ACGGGTGTGGTACGCAGCCCC′] 3815 NM_000321.2(RB1): c.2490 − 5925 RB1 [′AAGGGTGCTGTGGATCAGGGAAATGRTGA 1398A > G GTATGAAGCTGTTTTAAATTCT′] 3816 NM_000536.3(RAG2): c.115A > G 5897 RAG2 [′CTTTGGACAAAAAGGCTGGCCCAAARGAT (p.Arg39Gly) CCTGCCCCACTGGAGTTTTCCA′] 3817 NM_000448.2(RAG1): c.2735A > G 5896 RAG1 [′GAGTGCCCAGAATCCCTCTGCCAGTRCAGT (p.Tyr912Cys) TTCAATTCACAGCGTTTTGCT] 3818 NM_000448.2(RAG1): c.1286A > G 5896 RAG1 [′TTTGCTGACAAAGAAGAAGGTGGAGRTGT (p.Asp429Gly) GAAGTCCGTGTGCATGACCTTG′] 3819 NM_000925.3(PDHB): c.395A > G 5162 PDHB [′ATAAACTCAGCTGCCAAGACCTACTRCAT (p.Tyr132Cys) GTCTGGTGGCCTTCAGCCTGTG′] 3820 NM_000055.2(BCHE): c.293A > G 590 BCHE [′GCAAATTCTTGCTGTCAGAACATAGRTCAA (p.Asp98Gly) AGTTTTCCAGGCTTCCATGGA′] 3821 NM_000055.2(BCHE): c.467A > G 590 BCHE [′ACTGGAACATCATCTTTACATGTTTDTGAT (p.Tyr156Cys) GGCAAGTTTCTGGCTCGGGTT′] 3822 NM_005360.4(MAF): c.890A > G 4094 MAF [′CTGAAGCAGAAGAGGCGGACCCTGARAAA (p.Lys297Arg) CCGCGGCTATGCCCAGTCCTGC′] 3823 NM_002739.3(PRKCG): c.380A > G 5582 PRKCG [′TCCCTCCTCTACGGGCTTGTGCACCRGGGC (p.Gln127Arg) ATGAAATGCTCCTGTGAGTGA′] 3824 NM_002739.3(PRKCG): c.1081A > 5582 PRKCG [′CTTCCTCATGGTTCTAGGAAAAGGCRGTTT G (p.Ser361Gly) TGGGAAGGTTGGATTCCTGGG′] 3825 NM_000141.4(FGFR2): c.983A > G 2263 FGFR2 [′ACGGACAAAGAGATTGAGGTTCTCTRTATT (p.Tyr328Cys) CGGAATGTAACTTTTGAGGAC′] 3826 NM_000141.4(FGFR2): c.1124A > G 2263 FGFR2 [′AAGGAGATTACAGCTTCCCCAGACTRCCT (p.Tyr375Cys) GGAGATAGCCATTTACTGCATA′] 3827 NM_000141.4(FGFR2): c.874A > G 2263 FGFR2 [′TGCCCAGCCCCACATCCAGTGGATCRAGC (p.Lys292Glu) ACGTGGAAAAGAACGGCAGTAA′] 3828 NM_000141.4(FGFR2): c.1576A > G 2263 FGFR2 [′ACTGTTCATAGATGATGCCACAGAGRAAG (p.Lys526Glu) ACCTTTCTGATCTGGTGTCAGA′] 3829 NM_000313.3(PROS1): c.773A > G 5627 PROS1 [′AACATGTGTGCTCAGCTTTGTGTCARTTAC (p.Asn258Ser) CCTGGAGGTTACACTTGCTAT′] 3830 NM_000313.3(PROS1): c.586A > G 5627 PROS1 [′AAATGGTTTTGTTATGCTTTCAAATRAGAA (p.Lys196Glu) AGATTGTAAAGGTAAGAGCAG′] 3831 NM_002834.3(PTPN11): c.922A > G 5781 PTPN11 [′GCCTGTTTCAGATTACATCAATGCARATAT (p.Asn308Asp) CATCATGGTAAGCTTTGCTTT′] 3832 NM_002834.3(PTPN11): c.923A > G 5781 PTPN11 [′CCTGTTTCAGATTACATCAATGCAAVTATC (p.Asn308Ser) ATCATGGTAAGCTTTGCTTTT′] 3833 NM_002834.3(PTPN11): c.836A > G 5781 PTPN11 [′CAAGAAAACAAAAACAAAAATAGATVTA (p.Tyr279Cys) AAAACATCCTGCCCTGTAAGTAT′] 3834 NM_002834.3(PTPN11): c.182A > G 5781 PTPN11 [′CACATCAAGATTCAGAACACTGGTGNTTA (p.Asp61Gly) CTATGACCTGTATGGAGGGGAG′] 3835 NM_002834.3(PTPN11): c.188A > G 5781 PTPN11 [′AAGATTCAGAACACTGGTGATTACTRTGA (p.Tyr63Cys) CCTGTATGGAGGGGAGAAATTT′] 3836 NM_002834.3(PTPN11): c.227A > G 5781 PTPN11 [′GGGGAGAAATTTGCCACTTTGGCTGNGTT (p.Glu76Gly) GGTCCAGTATTACATGGAACAT′] 3837 NM_002834.3(PTPN11): c.236A > G 5781 PTPN11 [′TTTGCCACTTTGGCTGAGTTGGTCCRGTAT (p.Gln79Arg) TACATGGAACATCACGGGCAA′] 3838 NM_002834.3(PTPN11): c.1529A > 5781 PTPN11 [′TCAGGGATGGTCCAGACAGAAGCACVGTA G (p.Gln510Arg) CCGATTTATCTATATGGCGGTC′] 3839 NM_002755.3(MAP2K1): c.389A > 5604 MAP2K1 [′AACTCTCCGTACATCGTGGGCTTCTRTGGT G (p.Tyr130Cys) GCGTTCTACAGCGATGGCGAG′] 3840 NM_006006.4(ZBTB16): c.1849A > 7704 ZBTB16 [′CCAGCGCTCCCGGGACTACTCGGCCRTGAT G (p.Met617Val) CAAGCACCTGAGAACGCACAA′] 3841 NM_000311.3(PRNP): c.385A > G 5621 PRNP [′AGTGGTGGGGGGCCTTGGCGGCTACRTGC (p.Met129Val) TGGGAAGTGCCATGAGCAGGCC′] 3842 NM_000311.3(PRNP): c.650A > G 5621 PRNP [′GTGGTTGAGCAGATGTGTATCACCCRGTAC (p.Gln217Arg) GAGAGGGAATCTCAGGCCTAT′] 3843 NM_000311.3(PRNP): c.547A > G 5621 PRNP [′CTTTGTGCACGACTGCGTCAATATCRCAAT (p.Thr183Ala) CAAGCAGCACACGGTCACCAC′] 3844 NM_000311.3(PRNP): c.560A > G 5621 PRNP [′TGCGTCAATATCACAATCAAGCAGCRCAC (p.His187Arg) GGTCACCACAACCACCAAGGGG′] 3845 NM_000371.3(TTR): c.401A > G 7276 TTR [′ACCATTGCCGCCCTGCTGAGCCCCTRCTCC (p.Tyr134Cys) TATTCCACCACGGCTGTCGTC′] 3846 NM_000371.3(TTR): c.238A > G 7276 TTR [′GTCTGGAGAGCTGCATGGGCTCACARCTG (p.Thr80Ala) AGGAGGAATTTGTAGAAGGGAT′] 3847 NM_000371.3(TTR): c.185A > G 7276 TTR [′AGAAAGGCTGCTGATGACACCTGGGRGCC (p.Glu62Gly) ATTTGCCTCTGGGTAAGTTGCC′] 3848 NM_000371.3(TTR): c.205A > G 7276 TTR [′CCAGACTTTCACACCTTATAGGAAARCCA (p.Thr69Ala) GTGAGTCTGGAGAGCTGCATGG′] 3849 NM_000371.3(TTR): c.379A > G 7276 TTR [′CGACTCCGGCCCCCGCCGCTACACCRTTGC (p.Ile127Val) CGCCCTGCTGAGCCCCTACTC′] 3850 NM_000371.3(TTR): c.113A > G 7276 TTR [′TGTCCTCTGATGGTCAAAGTTCTAGRTGCT (p.Asp38Gly) GTCCGAGGCAGTCCTGCCATC′] 3851 NM_000217.2(KCNA1): c.676A > G 3736 KCNA1 [′CACAGACCCCTTCTTCATCGTGGAARCGCT (p.Thr226Ala) GTGTATCATCTGGTTCTCCTT′] 3852 NM_000217.2(KCNA1): c.763A > G 3736 KCNA1 [′GACGGACTTCTTCAAAAACATCATGRACTT (p.Asn255Asp) CATAGACATTGTGGCCATCAT′] 3853 NM_002693.2(POLG): c.2864A > G 5428 POLG [′AAAATCTTCAACTACGGCCGCATCTRTGGT (p.Tyr955Cys) GCTGGGCAGCCCTTTGCTGAG′] 3854 NM_002693.2(POLG): c.2591A > G 5428 POLG [′GAGCCCACATGGCTCACCGCCAGCARTGC (p.Asn864Ser) CCGGGTATGTGACCTCTGTACC′] 3855 NM_000174.4(GP9): c.182A > G 2815 GP9 [′ACCCGCCACCTTCTGCTGGCCAACARCAGC (p.Asn61Ser) CTTCAGTCCGTGCCCCCGGGA′] 3856 NM_000174.4(GP9): c.110A > G 2815 GP9 [′CTGGAAACCATGGGGCTGTGGGTGGRCTG (p.Asp37Gly) CAGGGGCCACGGACTCACGGCC′] 3857 NM_006206.4(PDGFRA): c.1664A > 5156 PDGFRA [′CCTGGTCATTTATAGAAACCGAGGTRTGA G (p.Tyr555Cys) AATTCGCTGGAGGGTCATTGAA′] 3858 NM_000301.3(PLG): c.112A > G 5340 PLG [′GGGGGCTTCACTGTTCAGTGTCACTRAGAA (p.Lys38Glu) GCAGCTGGGAGCAGGAAGTAT′] 3859 NM_000293.2(PHKB): c.306 − 2A > G 5257 PHKB [′GTTTCATGAGTTATCTCTCTCACCCRGGCG AATTGATGATGACAAGGGAAG′] 3860 NM_000175.3(GPI): c.1028A > G 2821 GPI [′ACACACGCCATGCTGCCCTATGACCRGTAC (p.Gln343Arg) CTGCACCGCTTTGCTGCGTAC′] 3861 NM_002633.2(PGM1): c.343A > G 5236 PGM1 [′CAAAGCCATTGGTGGGATCATTCTGRCAG (p.Thr115Ala) CCAGTCACAACCCAGGGGGCCC′] 3862 NM_006218.2(PIK3CA): c.3140A > 5290 PIK3CA [′TTCATGAAACAAATGAATGATGCACDTCA G (p.His1047Arg) TGGTGGCTGGACAACAAAAATG′] 3863 NM_006218.2(PIK3CA): c.1634A > 5290 PIK3CA [′CGAGATCCTCTCTCTGAAATCACTGVGCAG G (p.Glu545Gly) GAGAAAGATTTTCTATGGAGT′] 3864 NM_000478.4(ALPL): c.1250A > G 249 ALPL [′CCCTTCACTGCCATCCTGTATGGCARTGGG (p.Asn417Ser) CCTGGCTACAAGGTGGTGGGC′] 3865 NM_018849.2(ABCB4): c.523A > G 5244 ABCB4 [′AGAAATAAACAGGTATAAGATGTGARTTC - (p.Thr175Ala) AGTCCTCAAATAAACCTACTAT′, 3867 ′TTCTATATGAAAGTGTGACATTAACRATGT ACCTACTCTGTTAGCCGCGYA′, ′TTTCCTGTCGTAGAATAGCATGAAARAACT TCTGCCTAATTTTCCTGATCT′] 3868 NM_001083116.1(PRF1): c.755A > 5551 PRF1 [′CTGGCCCTGGAAGGGCTCACGGACARCGA G (p.Asn252Ser) GGTGGAGGACTGCCTGACTGTC′] 3869 NM_198965.1(PTHLH): c.534A > G 5744 PTHLH [′TTCCTTCTTTTTGCAGGAGGCATTGRAATT (p.Ter178T1p) TTCAGCAGAGACCTTCCAAGG′] 3870 NM_000316.2(PTH1R): c.668A > G 5745 PTH1R [′CTGCACTGCACGCGCAACTACATCCRCATG (p.His223Arg) CACCTGTTCCTGTCCTTCATG′] 3871 NM_003122.4(SPINK1): c.101A > G 6690 SPINK1 [′TTCCATTTTTAGGCCAAATGTTACARTGAA (p.Asn34Ser) CTTAATGGATGCACCAAGATA′] 3872 NM_006194.3(PAX9): c.271A > G 5083 PAX9 [′CGTGGTGAAACACATCCGGACCTACRAGC (p.Lys91Glu) AGAGAGACCCCGGCATCTTCGC′] 3873 NM_013953.3(PAX8): c.160A > G −1 — [′CGACATCTCTCGCCAGCTCCGCGTCRGCCA (p.Ser54Gly) TGGCTGCGTCAGCAAGATCCT′] 3874 NM_005188.3(CBL): c.1144A > G 867 CBL [′GATGGGCTCCACATTCCAACTATGTRAAAT (p.Lys382Glu) ATGTGCTGAAAATGATAAGGA′] 3875 NM_000222.2(KIT): c.2459A > G 3815 KIT [′GGTCTAGCCAGAGACATCAAGAATGRTTC (p.Asp820Gly) TAATTATGTGGTTAAAGGAAAC′] 3876 NM_000222.2(KIT): c.2386A > G 3815 KIT [′GTGTATTCACAGAGACTTGGCAGCCRGAA (p.Arg796Gly) ATATCCTCCTTACTCATGGTCG′] 3877 NM_000222.2(KIT): c.1924A > G 3815 KIT [′ACGGGAAGCCCTCATGTCTGAACTCRAAG (p.Lys642Glu) TCCTGAGTTACCTTGGTAATCA′] 3878 NM_001005862.2(ERBB2): c.2480A > 2064 ERBB2 [′CGGAACGTGCTGGTCAAGAGTCCCARCCA G (p.Asn827Ser) TGTCAAAATTACAGACTTCGGG′] 3879 NM_001127500.1(MET): c.3743A > 4233 MET [′GATTTTGGTCTTGCCAGAGACATGTRTGAT G (p.Tyr1248Cys) AAAGAATACTATAGTGTACAC′] 3880 NM_001127500.1(MET): c.3335A > 4233 MET [′ACAGGGCATTTTGGTTGTGTATATCVTGGG G (p.His1112Arg) ACTTTGTTGGACAATGATGGC′] 3881 NM_001127500.1(MET): c.3785A > 4233 MET [′AGTGTACACAACAAAACAGGTGCAARGCT G (p.Lys1262Arg) GCCAGTGAAGTGGATGGCTTTG′] 3882 NM_002524.4(NRAS): c.182A > G 4893 NRAS [′TTGGACATACTGGATACAGCTGGACNAGA (p.Gln6lArg) AGAGTACAGTGCCATGAGAGAC′] 3883 NM_004333.4(BRAF): c.1801A > G 673 BRAF [′AGGTGATTTTGGTCTAGCTACAGTGVAATC (p.Lys601Glu) TCGATGGAGTGGGTCCCATCA′] 3884 NM_004333.4(BRAF): c.1781A > G 673 BRAF [′GAAGACCTCACAGTAAAAATAGGTGDTTT (p.Asp594Gly) TGGTCTAGCTACAGTGAAATCT′] 3885 NM_004333.4(BRAF): c.770A > G 673 BRAF [′GACTTTTGTCGAAAGCTGCTTTTCCRGGGT (p.Gln257Arg) TTCCGCTGTCAAACATGTGGT′] 3886 NM_004333.4(BRAF): c.1495A > G 673 BRAF [′TACACCTCAGCAGTTACAAGCCTTCRAAA (p.Lys499Glu) ATGAAGTAGGAGTACTCAGGTG′] 3887 NM_004333.4(BRAF): c.1502A > G 673 BRAF [′CAGCAGTTACAAGCCTTCAAAAATGNAGT (p.Glu501Gly) AGGAGTACTCAGGTGAGCTTGT′] 3888 NM_004333.4(BRAF): c.1741A > G 673 BRAF [′CATCCACAGAGACCTCAAGAGTAATVGTA (p.Asn581Asp) TCCTTCCTGAAATTTGTCTGCG′] 3889 NM_000270.3(PNP): c.383A > G 4860 PNP [′CTGAACCCCAAGTTTGAGGTTGGAGRTATC (p.Asp128Gly) ATGCTGATCCGTGACCATATC′] 3890 NM_000270.3(PNP): c.575A > G 4860 PNP [′CAACGTGAGCTACAGGAAGGCACCTRTGT (p.Tyr192Cys) GATGGTGGCAGGCCCCAGCTTT′] 3891 NM_000258.2(MYL3): c.445A > G 4634 MYL3 [′CGACAAGGAGGGCAATGGCACTGTCRTGG (p.Met149Val) GTGCTGAGCTTCGCCACGTGCT′] 3892 NM_000257.3(MYH7): c.2333A > G 4625 MYH7 [′CTGGGGCTGCTGGAGGAAATGAGGGRCGA (p.Asp778Gly) GAGGCTGAGCCGCATCATCACG′] 3893 NM_000257.3(MYH7): c.2717A > G 4625 MYH7 [′CTGGCAGATGCTGAGGAGCGCTGTGRTCA (p.Asp906Gly) GCTGATCAAAAACAAGATTCAG′] 3894 NM_002470.3(MYH3): c.1385A > G 4621 MYH3 [′AGACAACACTTCATTGGTGTTTTGGRCATT (p.Asp462Gly) GCAGGCTTTGAAATCTTTGAG′] 3895 NM_000530.6(MPZ): c.286A > G 4359 MPZ [′CTACATTGACGAGGTGGGGACCTTCRAAG (p.Lys96Glu) AGCGCATCCAGTGGGTAGGGGA′] 3896 NM_000530.6(MPZ): c.242A > G 4359 MPZ [′TCCCCTCATTCCTCATAGATCTTCCRCTAT (p.His81Arg) GCCAAGGGACAACCCTACATT′] 3897 NG_012123.1: g.2493A > G 6347 CCL2 [′GAAAAGAAAGTCTTCTGGAAAGTGAYAGC TGTCTGCCTCCCACTTCTGCTC′] 3898 RMRP: n.71A > G 6023 RMRP [′TGTTCCTCCCCTTTCCGCCTAGGGGRAAGT CCCCGGACCTCGGGCAGAGAG′] 3899 NM_005006.6(NDUFS1): c.755A > 4719 NDUFS1 [′TTTCTCAGAAAGACAGAATCCATTGRTGTA G (p.Asp252Gly) ATGGATGCGGTTGGAAGTAAT′] 3900 NM_005912.2(MC4R): c.508A > G 4160 MC4R [′GACAGTTAAGCGGGTTGGGATCATCRTAA (p.Ile170Val) GTTGTATCTGGGCAGCTTGCAC′] 3901 NM_005912.2(MC4R): c.821A > G 4160 MC4R [′ATATTCTACATCTCTTGTCCTCAGARTCCA (p.Asn274Ser) TATTGTGTGTGCTTCATGTCT′] 3902 NM_005912.2(MC4R): c.289A > G 4160 MC4R [′GGCTGATATGCTGGTGAGCGTTTCARATGG (p.Asn97Asp) ATCAGAAACCATTGTCATCAC′] 3903 NM_005912.2(MC4R): c.185A > G 4160 MC4R [′CTGGGTGTCATCAGCTTGTTGGAGARTATC (p.Asn62Ser) TTAGTGATTGTGGCAATAGCC′] 3904 NM_000900.3(MGP): c.62 − 2A > G 4256 MGP [′AAGATTCCATGCTTTCATGTGATTCHGAAA TTAAAAAAAAAAGATTCATTA′] 3905 NM_012064.3(MEP): c.401A > G 4284 MEP [′AGCGTGGGCCAGGCAACCACAGTGGRGAT (p.Glu134Gly) CTTCCTGACGCTCCAGTTCGTG′] 3906 NM_000233.3(LHCGR): c.1733A > −1 — [′AAAATGGCAATCCTCATCTTCACCGRTTTC G (p.Asp578Gly) ACCTGCATGGCACCTATCTCT′] 3907 NM_000233.3(LHCGR): c.1691A > −1 — [′CCAGAATTAATGGCTACCAATAAAGRTAC G (p.Asp564Gly) AAAGATTGCTAAGAAAATGGCA′] 3908 NM_000894.2(LHB): c.221A > G 3972 LHB [′CAGGCGGTCCTGCCGCCCCTGCCTCRGGTG (p.Gln74Arg) GTGTGCACCTACCGTGATGTG′] 3909 NM_000238.3(KCNH2): c.1408A > 3757 KCNH2 [′CATGTTCATTGTGGACATCCTCATCRACTT G (p.Asn470Asp) CCGCACCACCTACGTCAATGC′] 3910 NM_001754.4(RUNX1): c.328A > G 861 RUNX1 [′GCTGCCTACGCACTGGCGCTGCAACRAGA (p.Lys110Glu) CCCTGCCCATCGCTTTCAAGGT′] 3911 NM_170707.3(LMNA): c.608A > G 4000 LMNA [′AACAGGCTGCAGACCATGAAGGAGGDACT (p.Glu203Gly) GGACTTCCAGAAGAACATCTAC′] 3912 NM_000421.3(KRT10): c.1315A > G −1 — [′TGAATACCAACAACTCCTGGATATTDAGA (p.Lys439Glu) TCCGACTGGAGAATGAAATTCA′] 3913 NM_000421.3(KRT10): c.1374 − −1 — [′CCGCCGCGTCCGCCGCCTCCGGAACYAAA 2A > G CGGGGTGAGGTCACATTCGGTT′] 3914 NM_000422.2(KRT17): c.274A > G 3872 KRT17 [′TGAGAAGGCCACCATGCAGAACCTCVATG (p.Asn92Asp) ACCGCCTGGCCTCCTACCTGGA′] 3915 NM_000422.2(KRT17): c.275A > G 3872 KRT17 [′GAGAAGGCCACCATGCAGAACCTCARTGA (p.Asn92Ser) CCGCCTGGCCTCCTACCTGGAC′] 3916 NM_005557.3(KRT16): c.374A > G 3868 KRT16 [′GAGAAGGTGACCATGCAGAACCTCARTGA (p.Asn125Ser) CCGCCTGGCCTCCTACCTGGAC′] 3917 NM_000526.4(KRT14): c.368A > G 3861 KRT14 [′GAGAAGGTGACCATGCAGAACCTCARTGA (p.Asn123Ser) CCGCCTGGCCTCCTACCTGGAC′] 3918 NM_000424.3(KRT5): c.1424A > G 3852 KRT5 [′ATCGCCACTTACCGCAAGCTGCTGGRGGG (p.Glu475Gly) CGAGGAATGCAGGTGAGTAGAC′] 3919 NM_000418.3(IL4R): c.223A > G 3566 IL4R [′CTGTGTCTGCAGAGCCCACACGTGTNTCCC (p.Ile75Val) TGAGAACAACGGAGGCGCGGG′] 3920 NM_001079817.1(INSR): c.1459A > 3643 INSR [′CCAGGAGAGAAACGACATTGCCCTGRAGA G (p.Lys487Glu) CCAATGGGGACCAGGCATCCTG′] 3921 NM_000208.2(INSR): c.707A > G 3643 INSR [′TGCACCGCCGAAGGCCTCTGTTGCCRCAGC (p.His236Arg) GAGTGCCTGGGCAACTGTTCT′] 3922 NM_000208.2(INSR): c.1466A > G 3643 INSR [′AGAAACGACATTGCCCTGAAGACCARTGG (p.Asn489Ser) GGACCAGGCATCCTGTAAGTCA′] 3923 NM_000208.2(INSR): c.1124 − 2A > G  3643 INSR [′CTTCTAGCTCAGCTGCCAGATTGTCYAAGG AAAGGAGAGAATATCCAGTGG′] 3924 NM_000208.2(INSR): c.1372A > G 3643 INSR [′TCAGGGGAAACTCTTCTTCCACTATRACCC (p.Asn458Asp) CAAACTCTGCTTGTCAGAAAT′] 3925 NM_000454.4(SOD1): c.131A > G 6647 SOD1 [′ATTAAAGGACTGACTGAAGGCCTGCRTGG (p.His44Arg) ATTCCATGTTCATGAGTTTGGA′] 3926 NM_000454.4(SOD1): c.302A > G 6647 SOD1 [′GATGGTGTGGCCGATGTGTCTATTGRAGAT (p.Glu101Gly) TCTGTGATCTCACTCTCAGGA′] 3927 NM_000454.4(SOD1): c.140A > G 6647 SOD1 [′CTGACTGAAGGCCTGCATGGATTCCRTGTT (p.His47Arg) CATGAGTTTGGAGATAATACA′] 3928 NM_000454.4(SOD1): c.242A > G 6647 SOD1 [′ATCTGATGCTTTTTCATTATTAGGCRTGTT (p.His81Arg) GGAGACTTGGGCAATGTGACT′] 3929 NM_000183.2(HADHB): c.788A > G 3032 HADHB [′GCACAGGATGAAGGACTCCTTTCTGRTGTG (p.Asp263Gly) GTACCCTTCAAAGTACCAGGT′] 3930 NM_000523.3(HOXD13): c.974A > 3239 HOXD13 [′GAGAGACAAGTGACCATTTGGTTTCRGAA G (p.Gln325Arg) CCGAAGAGTGAAGGACAAGAAA′] 3931 NM_000545.6(HNF1A): c.365A > G 6927 HNF1A [′CGTGTGGCGAAGATGGTCAAGTCCTRCCT (p.Tyr122Cys) GCAGCAGCACAACATCCCACAG′] 3932 NM_000519.3(HBD): c.-81A > G 3045 HBD [′CTGGAGCAGGGAGGACAGGACCAGCRTAA AAGGCAGGGCAGAGTCGACTGT′] 3933 NM_000518.4(HBB): c.199A > G 3043 HBB [′CCCTAAGGTGAAGGCTCATGGCAAGRAAG (p.Lys67Glu) TGCTCGGTGCCTTTAGTGATGG′] 3934 NM_000518.4(HBB): c.59A > G 3043 HBB [′GTTACTGCCCTGTGGGGCAAGGTGARCGT (p.Asn20Ser) GGATGAAGTTGGTGGTGAGGCC′] 3935 NM_000518.4(HBB): c.-81A > G 3043 HBB [′GGGAGGGCAGGAGCCAGGGCTGGGCVTA AAAGTCAGGGCAGAGCCATCTAT′] 3936 NM_000518.4(HBB): c.-50 − 29A > G 3043 HBB [′GAGGGCAGGAGCCAGGGCTGGGCATRAAA GTCAGGGCAGAGCCATCTATTG′] 3937 NM_000518.4(HBB): c.-78A > G 3043 HBB [′AGGGCAGGAGCCAGGGCTGGGCATAVAA GTCAGGGCAGAGCCATCTATTGC′] 3938 NM_000518.4(HBB): c.*113A > G 3043 HBB [′TGAGCATCTGGATTCTGCCTAATAARAAAC ATTTATTTTCATTGCAATGAT′] 3939 NM_000518.4(HBB): c.*112A > G 3043 HBB [′TTGAGCATCTGGATTCTGCCTAATADAAAA CATTTATTTTCATTGCAATGA′] 3940 NM_000518.4(HBB): c.*111A > G 3043 HBB [′CTTGAGCATCTGGATTCTGCCTAATRAAAA ACATTTATTTTCATTGCAATG′] 3941 NM_000518.4(HBB): c.80A > G 3043 HBB [′GTGAACGTGGATGAAGTTGGTGGTGNGGC (p.Glu27Gly) CCTGGGCAGGTTGGTATCAAGG′] 3942 NM_000518.4(HBB): c.247A > G 3043 HBB [′TGGCCTGGCTCACCTGGACAACCTCVAGG (p.Lys83Glu) GCACCTTTGCCACACTGAGTGA′] 3943 NM_000517.4(HBA2): c.1A > G 3040 HBA2 [′CCCACAGACTCAGAGAGAACCCACCRTGG (p.Met1Val) TGCTGTCTCCTGCCGACAAGAC′] 3944 NM_000517.4(HBA2): c.*92A > G 3040 HBA2 [′CACCGGCCCTTCCTGGTCTTTGAATRAAGT CTGAGTGGGCAGCAGCCTGTG′] 3945 NM_000517.4(HBA2): c.96 − 2A > G 3040 HBA2 [′CACCCCTCACTCTGCTTCTCCCCGCRGGAT GTTCCTGTCCTTCCCCACCAC′] 3946 NM_006121.3(KRT1): c.1445A > G 3848 KRT1 [′GCCCTGGATCTGGAGATTGCCACCTRCAG (p.Tyr482Cys) GACCCTCCTGGAGGGAGAAGAA′] 3947 NM_001077488.3(GNAS): c.1A > G 2778 GNAS [′GCGCCCCGCCGCCGCCGCCGCCGCCRTGG (p.Met1Val) GCTGCCTCGGGAACAGTAAGAC′] 3948 NM_000516.5(GNAS): c.680A > G 2778 GNAS [′ACCAGCATGTTTGACGTGGGTGGCCDGCG (p.Gln227Arg) CGATGAACGCCGCAAGTGGATC′] 3949 NM_000515.4(GH1): c.413A > G 2688 GH1 [′GGCGCCTCTGACAGCAACGTCTATGRCCTC (p.Asp138Gly) CTAAAGGACCTAGAGGAAGGC′] 3950 NM_000823.3(GHRHR): c.985A > G 2692 GHRHR [′TTGTCTTTCCTGCAGGCGTCTCTCCRAGTC (p.Lys329Glu) GACACTTTTCCTGATCCCACT′] 3951 NM_002890.2(RASA1): c.1198A > G 5921 RASA1 [′GACCAATGAAAATATTCAGCGATTTRAAA (p.Lys400Glu) TATGTCCAACGCCAAACAATCA′] 3952 NM_002890.2(RASA1): c.1201A > G 5921 RASA1 [′CAATGAAAATATTCAGCGATTTAAARTAT (p.Ile401Val) GTCCAACGCCAAACAATCAGTT′] 3953 NM_000406.2(GNRHR): c.317A > G 2798 GNRHR [′GATGGGATGTGGAACATTACAGTCCRATG (p.Gln106Arg) GTATGCTGGAGAGTTACTCTGC′] 3954 NM_000406.2(GNRHR): c.851A > G 2798 GNRHR [′TTTACTGTCTGCTGGACTCCCTACTRTGTC (p.Tyr284Cys) CTAGGAATTTGGTATTGGTTT′] 3955 NM_000407.4(GP1BB): c.338A > G −1 — [′GCCGGCCGCCCCGAGCGTGCGCCCTDCCG (p.Tyr113Cys) CGACCTGCGTTGCGTGGCGCCC′] 3956 NM_021957.3(GYS2): c.116A > G 2998 GYS2 [′TTTGAAGTTGCTTGGGAAGTGACCARTAA (p.Asn39Ser) AGGTTTGTACTGCTCCTGAAGG′] 3957 NM_001146040.1(GLRA1): c.920A > 2741 GLRA1 [′CCTCCACCCCCACTCTAGGTGTCCTVTGTG G (p.Tyr307Cys) AAAGCCATTGACATTTGGATG′] 3958 NM_001146040.1(GLRA1): c.910A > 2741 GLRA1 [′CTCCGGCTCTCGAGCATCTCTGCCCRAGGT G (p.Lys304Glu) AAGTCCCATTGCCCAAGAGCA′] 3959 NM_000171.3(GLRA1): c.523A > G 2741 GLRA1 [′CCCCATGGACTTGAAGAATTTCCCCRTGGA (p.Met175Val) TGTCCAGACATGTATCATGCA′] 3960 NM_000162.3(GCK): c.641A > G 2645 GCK [′ACGGTGGCCACGATGATCTCCTGCTRCTAC (p.Tyr214Cys) GAAGACCATCAGTGCGAGGTC′] 3961 NM_000145.3(FSHR): c.1345A > G 2492 FSHR [′AGGCTGTGATGCTGCTGGCTTTTTCRCTGT (p.Thr449Ala) CTTTGCCAGTGAGCTGTCAGT′] 3962 NM_182925.4(FLT4): c.3104A > G 2324 FLT4 [′CGCCTCCCCGCACCCCAGTGCATCCRCAGA (p.His1035Arg) GACCTGGCTGCTCGGAACATT′] 3963 NM_023110.2(FGFR1): c.1121A > G 2260 FGFR1 [′CCGGCAGTGATGACCTCGCCCCTGTRCCTG (p.Tyr374Cys) GAGATCATCATCTATTGCACA′] 3964 NM_006894.5(FMO3): c.182A > G 2328 FMO3 [′AGCATTTACAAATCAGTCTTTTCCARCTCT (p.Asn61Ser) TCCAAAGAGATGATGTGTTTC′] 3965 NM_001002294.2(FMO3): c.923A > 2328 FMO3 [′AAGCCTAACGTGAAGGAATTCACAGRGAC G (p.Glu308Gly) CTCGGCCATTTTTGAGGATGGG′] 3966 NM_212482.1(FN1): c.2918A > G 2335 FN1 [′ACCGGGCTGTCCCCTGGGGTCACCTRTTAC (p.Tyr973Cys) TTCAAAGTCTTTGCAGTGAGC′] 3967 NM_000142.4(FGFR3): c.1948A > G 2261 FGFR3 [′CGTGCACAACCTCGACTACTACAAGVAGA (p.Lys650Glu) CAACCAACGTGAGCCCGGCCCT′] 3968 NM_000142.4(FGFR3): c.1118A > G 2261 FGFR3 [′GAGGCTGACGAGGCGGGCAGTGTGTRTGC (p.Tyr373Cys) AGGCATCCTCAGCTACGGGGTG] 3969 NM_000142.4(FGFR3): c.1612A > G 2261 FGFR3 [′GTGCAGGCGCCCAGCAGGTTGATGAYGTT (p.Ile538Val) TTTGTGTTTCCCGATCATCTTC′] 3970 NM_000142.4(FGFR3): c.1619A > G 2261 FGFR3 [′GCCCTGCGTGCAGGCGCCCAGCAGGBTGA (p.Asn540Ser) TGATGTTTTTGTGTTTCCCGAT′] 3971 NM_000142.4(FGFR3): c.833A > G 2261 FGFR3 [′GACGTGGAGTTCCACTGCAAGGTGTRCAG (p.Tyr278Cys) TGACGCACAGCCCCACATCCAG] 3972 NM_000138.4(FBN1): c.6431A > G 2200 FBN1 [′GTCTGTAAACATGGACAGTGCATCARTAC (p.Asn2144Ser) AGATGGTTCCTATCGCTGCGAG] 3973 NM_000138.4(FBN1): c.3128A > G 2200 FBN1 [′ATACCCAGCCTCTGCACCCACGGCARGTG (p.Lys1043Arg) CAGAAACACCATTGGCAGCTTT′] 3974 NM_000138.4(FBN1): c.2261A > G 2200 FBN1 [′TATAAATGTATATGCAATTCAGGATRTGAA (p.Tyr754Cys) GTGGATTCAACTGGGAAAAAC′] 3975 NM_000146.3(FTL): c.-160A > G 2512 FTL [′CCGCGGGTCTGTCTCTTGCTTCAACRGTGT TTGGACGGAACAGATCCGGGG′] 3976 NM_000043.4(FAS): c.695A > G 355 FAS [′TTTTCAGATGTTGACTTGAGTAAATRTATC (p.Tyr232Cys) ACCACTATTGCTGGAGTCATG] 3977 NM_000043.4(FAS): c.763A > G 355 FAS [′AGGCTTTGTTCGAAAGAATGGTGTCRATG (p.Asn255Asp) AAGCCAAAATAGATGAGATCAA′] 3978 NM_000043.4(FAS): c.353A > G 355 FAS [′TTTCTAGGCTTAGAAGTGGAAATAARCTGC (p.Asn118Ser) ACCCGGACCCAGAATACCAAG′] 3979 NM_000129.3(F13A1): c.851A > G 2162 F13A1 [′CTCGTTGGATCCTGGGACAATATCTRTGCC (p.Tyr284Cys) TATGGCGTCCCCCCATCGGCC′] 3980 NM_000121.3(EPOR): c.1460A > G 2057 EPOR [′GGCTTATCCGATGGCCCCTACTCCARCCCT (p.Asn487Ser) TATGAGAACAGCCTTATCCCA′] 3981 NM_207034.2(EDN3): c.335A > G 1908 EDN3 [′GACAAGGAGTGTGTCTACTATTGCCRCCTG (p.His112Arg) GACATCATTTGGATCAACACT′] 3982 NM_001257988.1(TYMP): c.665A > 1890 TYMP [′CAGAGCGGACAGCCCCTCCACGAGTYTCT G (p.Lys222Arg) TACTGAGAATGGAGGCTTTGGG′] 3983 NM_003286.2(TOP1): c.1598A > G −1 — [′GTAGAGTTTGACTTCCTCGGGAAGGRCTCC (p.Asp533Gly) ATCAGATACTATAACAAGGTC′] 3984 NM_001943.3(DSG2): c.797A > G 1829 DSG2 [′GTTCAGATTCGTATTTTGGATGTCARTGAC (p.Asn266Ser) AATATACCTGTAGTAGAAAAT′] 3985 NM_001943.3(DSG2): c.1880 − 1829 DSG2 [′TCTGTGTTCAATTTTGTGTCTGTACRGTGG 2A > G TACCACTTTTACTGCTGATGT′] 3986 NM_024422.4(DSC2): c.631 − 2A > G 1824 DSC2 [′TTTCTCCTTATTTTACTCTCTTCACRGATAA TTGCCTTTGCAACAACTCCA′] 3987 NG_012088.1: g.2209A > G 3586 IL10 [′CCAGGATGGTCTCGATCTCCTGACCYTATG ATCCGCCCGCCTTGGCCTCCC′] 3988 NM_000498.3(CYP11B2): c.1492A > −1 — [′GAGGCCTGGCACGTCCCCCCTCCTCRCTTT G (p.Thr498Ala) CAGAGCGATTAACTAGTCTTG′] 3989 NM_000941.2(POR): c.1733A > G 5447 POR [′CGCCGCTCGGATGAGGACTACCTGTRCCG (p.Tyr578Cys) GGAGGAGCTGGCGCAGTTCCAC′] 3990 NM_000941.2(POR): c.15A > G 5447 POR [′CTAACAGTTTCATGATCAACATGGGRGACT (p.Gly5=) CCCACGTGGACACCAGCTCCA′] 3991 NM_001885.2(CRYAB): c.358A > G 1410 CRYAB [′TGGTTTCATCTCCAGGGAGTTCCACRGGAA (p.Arg120Gly) ATACCGGATCCCAGCTGATGT′] 3992 NM_021954.3(GJA3): c.188A > G 2700 GJA3 [′AACACCCAGCAGCCGGGCTGCGAGARCGT (p.Asn63Ser) CTGCTACGACAGGGCCTTCCCC′] 3993 NM_004004.5(GJB2): c.218A > G 2706 GJB2 [′TACGATCACTACTTCCCCATCTCCCRCATC (p.His73Arg) CGGCTATGGGCCCTGCAGCTG′] 3994 NM_001735.2(C5): c.1115A > G 727 C5 [′CGTCTACCCCCTCACCCAATCTACCYTGAT (p.Lys372Arg) GGGATATGGAATCCCAGGCTT′] 3995 NM_001848.2(COL6A1): c.362A > 1291 COL6A1 [′CTCAAAAGCAGCGTGGACGCGGTCARGTA G (p.Lys121Arg) CTTTGGGAAGGGCACCTACACC′] 3996 NM_000093.4(COL5A1): c.655 − 1289 COL5A1 [′TTTTCATGAGCGTCTCTTCTTTTCCRGGGTG 2A > G ACATCCAGCAGCTGCTCTTT′] 3997 NM_000089.3(COL1A2): c.226 − 1278 COL1A2 [′TATAATTTTTTTTTTTTACTTCTCTRGAACT 2A > G TTGCTGCTCAGTATGATGGA′] 3998 NM_000089.3(COL1A2): c.70 + 717A > 1278 COL1A2 [′GAGTGGGTACATTCTGAAAAGTAATRTAA G GTGTCTCAATTCACTTTCTAGT′] 3999 NM_001844.4(COL2A1): c.4172A > 1280 COL2A1 [′ACGGAAGGCTCCCAGAACATCACCTRCCA G (p.Tyr1391Cys) CTGCAAGAACAGCATTGCCTAT′] 4000 NM_001844.4(COL2A1): c.2974A > 1280 COL2A1 [′CGGTCTGCCTGGGCAACGTGGTGAGRGAG G (p.Arg992Gly) GATTCCCTGGCTTGCCTGGCCC′] 4001 NM_000493.3(COL10A1): c.1790A > −1 — [′TTTACTTGTCAGATACCAGGAATATRCTAT G (p.Tyr597Cys) TTTTCATACCACGTGCATGTG′] 4002 NM_004385.4(VCAN): c.4004 − 1462 VCAN [′GTATTGTGAAAACTCTGTTTTTTTCDGGTC 2A > G GAATGAGTGATTTGAGTGTAA′] 4003 NM_020549.4(CHAT): c.1444A > G 1103 CHAT [′CGTCAGCGAGCTCCCCGCCCCCCGGVGGC (p.Arg482Gly) TGCGGTGGAAATGCTCCCCGGA′] 4004 NM_000078.2(CETP): c.1376A > G 1071 CETP [′AACAGCAAAGGCGTGAGCCTCTTCGRCAT (p.Asp459Gly) CATCAACCCTGAGATTATCACT′] 4005 NM_000083.2(CLCN1): c.1655A > G 1180 CLCN1 [′GTGATTTGCTTCGAATTAACGGGTCDGATT (p.Gln552Arg) GCTCACATCCTGCCCATGATG′] 4006 NM_000083.2(CLCN1): c.382A > G 1180 CLCN1 [′CTTTCTGGTGCTTCTGGGACTGCTGRTGGC (p.Met128Val) TCTGGTCAGCTGGAGCATGGA′] 4007 NM_199440.1(HSPD1): c.86A > G 3329 HSPD1 [′CATCTCACTCGGGCTTATGCCAAAGRTGTA (p.Asp29Gly) AAATTTGGTGCAGATGCCCGA′] 4008 NM_001904.3(CTNNB1): c.95A > G 1499 CTNNB1 [′CACTGGCAGCAACAGTCTTACCTGGNCTCT (p.Asp32Gly) GGAATCCATTCTGGTGCCACT′] 4009 NM_001904.3(CTNNB1): c.121A > 1499 CTNNB1 [′CTCTGGAATCCATTCTGGTGCCACTNCCAC G (p.Thr41Ala) AGCTCCTTCTCTGAGTGGTAA′] 4010 NM_007294.3(BRCA1): c.211A > G 672 BRCA1 [′TTTATGTAAGAATGATATAACCAAARGGT (p.Arg71Gly) ATATAATTTGGTAATGATGCTA′] 4011 NM_001202.3(BMP4): c.278A > G 652 BMP4 [′GATCTTTACCGGCTTCAGTCTGGGGRGGAG (p.Glu93Gly) GAGGAAGAGCAGATCCACAGC′] 4012 NM_000342.3(SLC4A1): c.166A > G 6521 SLC4A1 [′CACCACATCACACCCGGGTACCCACRAGG (p.Lys56Glu) TGAGGACCCCAGCCTCCTCCGT′] 4013 NM_000342.3(SLC4A1): c.2509A > 6521 SLC4A1 [′GAAGACCTGGCGCATGCACTTATTCRCGG G (p.Thr837Ala) GCATCCAGATCATCTGCCTGGC′] 4014 NM_001681.3(ATP2A2): c.2300A > 488 ATP2A2 [′TTCATCCGCTACCTCATCTCGTCCARCGTC G (p.Asn767Ser) GGGGAAGTTGTCTGGTAGGTC′] 4015 NM_000486.5(AQP2): c.203A > G 359 AQP2 [′GGCCACATAAGCGGGGCCCACATCAVCCC (p.Asn68Ser) TGCCGTGACTGTGGCCTGCCTG′] 4016 NM_000041.3(APOE): c.237 − 2A > G 348 APOE [′GACACCCTCCCGCCCTCTCGGCCGCRGGGC GCTGATGGACGAGACCATGAA′] 4017 NM_000041.3(APOE): c.490A > G 348 APOE [′CGCCTCCCACCTGCGCAAGCTGCGTVAGC (p.Lys164Glu) GGCTCCTCCGCGATGCCGATGA′] 4018 NM_000041.3(APOE): c.178A > G 348 APOE [′TTGGGATTACCTGCGCTGGGTGCAGRCACT (p.Thr60Ala) GTCTGAGCAGGTGCAGGAGGA′] 4019 NM_000040.1(APOC3): c.280A > G 345 APOC3 [′GGATTTGGACCCTGAGGTCAGACCARCTTC (p.Thr94Ala) AGCCGTGGCTGCCTGAGACCT′] 4020 NM_000040.1(APOC3): c.232A > G 345 APOC3 [′CCTGAAAGACTACTGGAGCACCGTTRAGG (p.Lys78Glu) ACAAGTTCTCTGAGTTCTGGGA′] 4021 NM_001042425.1(TFAP2A): c.751A > 7020 TFAP2A [′GTCGCTGCTGGGCGGAGTGCTCCGGVGGT G (p.Arg251Gly) GAGGCCCGGCACGGCCCCGCCC′] 4022 NM_000488.3(SERPINC1): c.655A > 462 SERPINC1 [′TGCAGAGCAATCCAGAGCGGCCATCRACA G (p.Asn219Asp) AATGGGTGTCCAATAAGACCGA′] 4023 NM_001085.4(SERPINA3): c.1240A > 12 SERPINA3 [′TACAGACACCCAGAACATCTTCTTCRTGAG G (p.Met414Val) CAAAGTCACCAATCCCAAGCA′] 4024 NM_001148.4(ANK2): c.4373A > G 287 ANK2 [′TGCATGGCATCTTGGGGCGGAAAGGRATC (p.Glu1458Gly) AGAGTCAGATCAAGAACAGGAG′] 4025 NM_001145.4(ANG): c.121A > G −1 — [′CTTCCTGACCCAGCACTATGATGCCRAACC (p.Lys41Glu) ACAGGGCCGGGATGACAGATA′] 4026 NM_001145.4(ANG): c.208A > G −1 — [′ACCCTGCAAAGACATCAACACATTTRTTCA (p.Ile70Val) TGGCAACAAGCGCAGCATCAA′] 4027 NM_000484.3(APP): c.2146A > G 351 APP [′GGGCGGTGTTGTCATAGCGACAGTGRTCG (p.Ile716Val) TCATCACCTTGGTGATGCTGAA′] 4028 NM_000484.3(APP): c.2078A > G 351 APP [′TTTGTTTTCAAGGTGTTCTTTGCAGRAGAT (p.Glu693Gly) GTGGGTTCAAACAAAGGTGCA′] 4029 NM_000484.3(APP): c.2140A > G 351 APP [′CATGGTGGGCGGTGTTGTCATAGCGRCAG (p.Thr714Ala) TGATCGTCATCACCTTGGTGAT′] 4030 NM_000021.3(PSEN1): c.488A > G 5663 PSEN1 [′AAATGCTTTCTTTTCTAGGTCATCCRTGCC (p.His163Arg) TGGCTTATTATATCATCTCTA′] 4031 NM_000021.3(PSEN1): c.415A > G 5663 PSEN1 [′GCACTCAATTCTGAATGCTGCCATCRTGAT (p.Met139Val) CAGTGTCATTGTTGTCATGAC′] 4032 NM_000021.3(PSEN1): c.436A > G 5663 PSEN1 [′CATCATGATCAGTGTCATTGTTGTCNTGAC (p.Met146Val) TATCCTCCTGGTGGTTCTGTA′] 4033 NM_000021.3(PSEN1): c.839A > G 5663 PSEN1 [′GTTGAAACAGCTCAGGAGAGAAATGVAAC (p.Glu280Gly) GCTTTTTCCAGCTCTCATTTAC′] 4034 NM_000021.3(PSEN1): c.998A > G 5663 PSEN1 [′CAAGACACTGTTGCAGAGAATGATGRTGG (p.Asp333Gly) CGGGTTCAGTGAGGAATGGGAA′] 4035 NM_000034.3(ALDOA): c.386A > G 226 ALDOA [′CCCCTTCCTCTTCTCTTAGGGTTGGRTGGG (p.Asp129Gly) CTGTCTGAGCGCTGTGCCCAG′] 4036 NM_000477.5(ALB): c.714 − 2A > G 213 ALB [′CATTTTGATTGGCGATTTTCTTTTTDGGGC AGTAGCTCGCCTGAGCCAGAG′] 4037 NM_001151.3(SLC25A4): c.311A > 291 SLC25A4 [′AAGCAGCTCTTCTTAGGGGGTGTGGRTCG G (p.Asp104Gly) GCATAAGCAGTTCTGGCGCTAC′] 4038 NM_013411.4(AK2): c.1A > G 204 AK2 [′GTGGCAGTGAGAGACTTCGGCGGACRTGG (p.Met1Val) CTCCCAGCGTGCCAGCGGCAGA′] 4039 NM_013411.4(AK2): c.494A > G 204 AK2 [′AACCCTCCAAAAGAGCCCATGAAAGRTGA (p.Asp165Gly) CGTATGTAAACTCAGGACAAAA′] 4040 NM_000476.2(AK1): c.491A > G 203 AK1 [′GCCACAGAACCCGTCATCGCCTTCTRTGAG (p.Tyr164Cys) AAACGTGGCATTGTGCGCAAG′] 4041 NM_001100.3(ACTA1): c.350A > G 58 ACTA1 [′GAGGCCCCCCTCAATCCCAAGGCCARCCG (p.Asn117Ser) CGAGAAGATGACCCAGATCATG′] 4042 NM_001103.3(ACTN2): c.26A > G 88 ACTN2 [′ATGAACCAGATAGAGCCCGGCGTGCRGTA (p.Gln9Arg) CAACTACGTGTACGACGAGGAT′] 4043 NM_005159.4(ACTC1): c.1088A > G −1 — [′CAGCAAATGTGGATTAGCAAGCAAGRGTA (p.Glu363Gly) CGATGAGGCAGGCCCATCCATT′] 4044 NM_005159.4(ACTC1): c.373A > G −1 — [′CAACCGGGAGAAGATGACTCAGATCRTGT (p.Met125Val) TTGAGACCTTCAATGTCCCTGC′] 4045 NM_018109.3(MTPAP): c.1432A > 55149 MTPAP [′TGATTCTTCTCCTCTGTACATTCAGRATCC G (p.Asn478Asp) ATTTGAAACTTCTCTCAACAT′] 4046 NM_016955.3(SEPSECS): c.1001A > 51091 SEPSECS [′TTATTGTCACTTGGATCAAATGGCTRTAAG G (p.Tyr334Cys) AAGCTACTAAAAGAAAGAAAG′] 4047 NM_014053.3(FLVCR1): c.361A > 28982 FLVCR1 [′GATCTTCAGCCTGTACTCGCTGGTCRACGC G (p.Asn121Asp) CTTTCAGTGGATCCAGTACAG′] 4048 NM_000021.3(PSEN1): c.697A > G 5663 PSEN1 [′ATATCTCATTATGATTAGTGCCCTCDTGGC (p.Met233Val) CCTGGTGTTTATCAAGTACCT′] 4049 NM_000207.2(INS): c.323A > G −1 — [′TGCTCCCTCTACCAGCTGGAGAACTRCTGC (p.Tyr108Cys) AACTAGACGCAGCCCGCAGGC′] 4050 NM_000334.4(SCN4A): c.4078A > G 6329 SCN4A [′GAAGCAGGCCTTCGACATCACCATCRTGA (p.Met1360Val) TCCTCATCTGCCTCAACATGGT′] 4051 NM_000334.4(SCN4A): c.4108A > G 6329 SCN4A [′CCTCATCTGCCTCAACATGGTCACCRTGAT (p.Met1370Val) GGTGGAGACAGACAACCAGAG′] 4052 NM_000518.4(HBB): c.316 − 2A > G 3043 HBB [′TTCATACCTCTTATCTTCCTCCCACVGCTCC TGGGCAACGTGCTGGTCTGT′] 4053 NM_000525.3(KCNJ11): c.155A > G 3767 KCNJ11 [′GTGGCCCACAAGAACATCCGGGAGCRGGG (p.Gln52Arg) CCGCTTCCTGCAGGACGTGTTC′] 4054 NM_000525.3(KCNJ11): c.544A > G 3767 KCNJ11 [′AGCCCACCGCAGGGCTGAGACCCTCRTCTT (p.Ile182Val) CAGCAAGCATGCGGTGATCGC′] 4055 NM_000525.3(KCNJ11): c.886A > G 3767 KCNJ11 [′GGAAGGCGTGGTGGAAACCACGGGCVTCA (p.Ile296Val) CCACCCAGGCCCGCACCTCCTA′] 4056 NM_000525.3(KCNJ11): c.989A > G 3767 KCNJ11 [′GAGGACGGACGTTACTCTGTGGACTRCTCC (p.Tyr330Cys) AAGTTTGGCAACACCGTCAAA′] 4057 NM_000528.3(MAN2B1): c.1831 − 4125 MAN2B1 [′CCCATCTGTGGACCCTTTTCTGCCCRGCAC 2A > G ATCCGGGCAACGTTTGATCCT′] 4058 NM_000639.2(FASLG): c.466A > G 356 FASLG [′ATTTATTTCAGGCAAGTCCAACTCARGGTC (p.Arg156Gly) CATGCCTCTGGAATGGGAAGA′] 4059 NM_000920.3(PC): c.1705A > G 5091 PC [′CCCGGGGCTGCTGCTGATGGACACGRCCTT (p.Thr569Ala) CAGGGACGCCCACCAGTCACT′] 4060 NM_001017420.2(ESCO2): c.1132 − 157570 ESCO2 [′AATGGGTTTCTTTTTTTACCCCCCARTTATA 7A > G GGACGCTGGTCAGAAACATT′] 4061 NM_001017420.2(ESCO2): c.1674 − 157570 ESCO2 [′ATTAAATCATCTTTTCTTCTCTTTTRGGAAT 2A > G TGCTTCATGTTTGGCTGTTT′] 4062 NM_001129765.1(NSDHL): c.1046A > 50814 NSDHL [′GAGAGAGCCAAAAAGGCCATGGGCTVCCA G (p.Tyr349Cys) GCCACTAGTGACCATGGATGAT′] 4063 NM_001701.3(BAAT): c.967A > G 570 BAAT [′AGAGGCCCAGGGGCAATTCCTCTTCRTTGT (p.Ile323Val) AGGAGAAGGTGATAAGACTAT′] 4064 NM_002977.3(SCN9A): c.406A > G 6335 SCN9A [′ATTCAGCATGCTCATCATGTGCACTRTTCT (p.Ile136Val) GACAAACTGCATATTTATGAC′] 4065 NM_004004.5(GJB2): c.487A > G 2706 GJB2 [′CTATGTCATGTACGACGGCTTCTCCRTGCA (p.Met163Val) GCGGCTGGTGAAGTGCAACGC′] 4066 NM_004519.3(KCNQ3): c.1403A > 3786 KCNQ3 [′GAACCAAAGCCTGTTGGCTTAAACARTAA G (p.Asn468Ser) AGAGCGTTTCCGCACGGCCTTC′] 4067 NM_004519.3(KCNQ3): c.2462A > 3786 KCNQ3 [′AGAGATGATTATGTGTTCGGCCCCARTGG G (p.Asn821Ser) GGGGTCGAGCTGGATGAGGGAG′] 4068 NM_004519.3(KCNQ3): c.914A > G 3786 KCNQ3 [′AAAGAGGAGTTTGAGACCTATGCAGRTGC (p.Asp305Gly) CCTGTGGTGGGGCCTGGTGAGT′] 4069 NM_004525.2(LRP2): c.770 − 2A > G 4036 LRP2 [′TGTAACTCTCTCTTTTTCCCCCCACRGAAA GCGGTCCTCATGATGTTCATA′] 4070 NM_007375.3(TARDBP): c.1055A > 23435 TARDBP [′CAGAACCAGTCAGGCCCATCGGGTARTAA G (p.Asn352Ser) CCAAAACCAAGGCAACATGCAG′] 4071 NM_007375.3(TARDBP): c.800A > 23435 TARDBP [′AATGCCGAACCTAAGCACAATAGCARTAG G (p.Asn267Ser) ACAGTTAGAAAGAAGTGGAAGA′] 4072 NM_012434.4(SLCl7A5): c.406A > 26503 SLC17A5 [′ATATGTTGCCAGCAAAATAGGGGGGDAAA G (p.Lys136Glu) TGCTGCTAGGATTTGGGATCCT′] 4073 NM_012463.3(ATP6V0A2): c.732 − 23545 ATP6V0A2 [′AGACTGTGTTCAACTCTTGTCTTCCRGCTA 2A > G CCACTGCCACGTGTACCCCTA′] 4074 NM_014043.3(CHMP2B): c.85A > G 25978 CHMP2B [′GTTACGAGGTACACAGAGGGCTATARTCA (p.Ile29Val) GAGATCGAGCAGCTTTAGAGAA′] 4075 NM_018136.4(ASPM): c.2761 − 259266 ASPM [′AGCAAAATTATAATATTGGAATATARTATC 25A > G TGGAACTTATTTCTTTATAGG′] 4076 NM_022081.5(HPS4): c.461A > G 89781 HPS4 [′ATTCTGAAAAACACCAGTGATCTGCRTAA (p.His154Arg) GATTTTCAATTCCCTCTGGAAC′] 4077 NM_024649.4(BBS1): c.1340 − −1 — [′GTCAGCCTCTGGGACCCTTCTCCACRGCCA 2A > G TGCACCGGGCCTTCCAGACAG′] 4078 NM_032520.4(GNPTG): c.610 − 84572 GNPTG [′TGCTGCCCCTGCATCCTCCACCTTCRGGGC 2A > G CATGAGAAGTTGCTGAGGACA′] 4079 NM_172107.2(KCNQ2): c.1A > G 3785 KCNQ2 [′CCCGGGGCGCCTCCCGCCAGGCACCRTGG (p.MetlVal) TGCAGAAGTCGCGCAACGGCGG′] 4080 NM_172107.2(KCNQ2): c.356A > G 3785 KCNQ2 [′GTGTTTTCCACCATCAAGGAGTATGRGAA (p.Glu119Gly) GAGCTCGGAGGGGGCCCTCTAC′] 4081 NM_172107.2(KCNQ2): c.622A > G 3785 KCNQ2 [′CCTGCGCTTCCTGCAGATTCTGCGGRTGAT (p.Met208Val) CCGCATGGACCGGCGGGGAGG′] 4082 NM_172107.2(KCNQ2): c.635A > G 3785 KCNQ2 [′CAGATTCTGCGGATGATCCGCATGGRCCG (p.Asp212Gly) GCGGGGAGGCACCTGGAAGCTG′] 4083 NM_172107.2(KCNQ2): c.773A > G 3785 KCNQ2 [′GTGTACTTGGCAGAGAAGGGGGAGARCGA (p.Asn258Ser) CCACTTTGACACCTACGCGGAT′] 4084 NM_172107.2(KCNQ2): c.1764 − 3785 KCNQ2 [′ATGCTTTGGGGTCTCTGTTCCCGGTRGAGT 2A > G GGACCAGATCGTGGGGCGGGG′] 4085 NM_000495.4(COL4A5): c.1A > G 1287 COL4A5 [′AAGGAGCTGCGGGAGCCGGAGAAGARTG (p.Met1Val) AAACTGCGTGGAGTCAGCCTGGC′] 4086 NM_000495.4(COL4A5): c.466 − 1287 COL4A5 [′AGAACTTCCATTGATGGCTTCTTTTRGGGT 2A > G GAACCAGGTAGTATAATTATG′] 4087 NM_000495.4(COL4A5): c.547 − 1287 COL4A5 [′TCATTTTCTTTACTCACTTTATAACRGGGC 2A > G CTACCTGGTCCCACTGGTATA′] 4088 NM_000495.4(COL4A5): c.610 − 1287 COL4A5 [′ATTTTCTCTTTTGTCTTCTCTTCTTRGGGCC 2A > G CTCCTGGTCCACCAGGACTT′] 4089 NM_000495.4(COL4A5): c.892 − 1287 COL4A5 [′GCCCTATCATTTCTTTGTATCCTATRGGGT 2A > G AAACCAGGCAAAGATGGAGAA′] 4090 NM_000495.4(COL4A5): c.1340 − 1287 COL4A5 [′TTGCTATCCTTTCTTTATCTTACTCRGGTGA 2A > G TGAGATATGTGAACCAGGCC′] 4091 NM_000495.4(COL4A5): c.2042 − 1287 COL4A5 [′TTCTTTGAACGTTTTCCTTTCAATARCTGCT 18A > G GTTTCTCCATAGGTGACCCT′] 4092 NM_000495.4(COL4A5): c.2147 − 1287 COL4A5 [′GTTAAAAAATGACTTATCATTTTACRGGCT 2A > G TTCCTGGAATTCCAGGACCTC′] 4093 NM_000495.4(COL4A5): c.2394A > 1287 COL4A5 [′GCTTAGATGGGCTCCCTGGACCAAARGGT G (p.Lys798=) ATGGAGGCTGTCACTGCATCTC] 4094 NM_000495.4(COL4A5): c.2510 − 1287 COL4A5 [′CAGATACATCTTTTAAAACTGCTTCRGTAC 33A > G TTATTAATATTGATATTGTAT′] 4095 NM_000495.4(COL4A5): c.2692A > 1287 COL4A5 [′ATTCTTCAAAGGTACCAAAGGTGAARTGG G (p.Met898Val) GTATGATGGGACCTCCAGGCCC′] 4096 NM_000495.4(COL4A5): c.2746A > 1287 COL4A5 [′AGGACCTTTGGGAATTCCTGGCAGGRGTG G (p.Ser916Gly) GTGTACCTGGTCTTAAAGGTAA′] 4097 NM_000495.4(COL4A5): c.3107 − 1287 COL4A5 [′ATATTATATATCACATATTTTCAACRGGGC 2A > G CTCAGGGTGTGGAAGGGCCTC′] 4098 NM_000495.4(COL4A5): c.3455 − 1287 COL4A5 [′AACTGGGTGTAACCTGCTGTACTCARTTTT 9A > G TTAGGTGGTGGAGGTCATCCT′] 4099 NM_000495.4(COL4A5): c.3605 − 1287 COL4A5 [′TCTTCTAATTATACTTTACTTTCATRGGCCA 2A > G AAAGGGTGATGGAGGATTAC′] 4100 NM_000495.4(COL4A5): c.3925 − 1287 COL4A5 [′GTAACATTAATGATTTTATTTATTCRGGGT 2A > G AATCCTGGCCGGCCGGGTCTC′] 4101 NM_000495.4(COL4A5): c.3998 − 1287 COL4A5 [′ATAAAACTGTATGTACCTTCTGTGCDGGCA 2A > G TGAAAGGACCCAGTGGAGTAC′] 4102 NM_000495.4(COL4A5): c.4790A > 1287 COL4A5 [′GGATGGGATTCTCTGTGGATTGGTTRTTCC G (p.Tyr1597Cys) TTCATGATGGTATTTTACACT′] 4103 NM_000495.4(COL4A5): c.4977 − 1287 COL4A5 [′CTGATTGTCTTATTTCTTATTTCCCRGTAAA 2A > G CCTCAGTCAGAAACGCTGAA′] 4104 NM_005359.5(SMAD4): c.425 − 4089 SMAD4 [′TTTCATTTGTTTTCCCCTTTAAACARTTAAG 6A > G ATCTCTCAGGATTAACACTG′] 4105 NM_005359.5(SMAD4): c.989A > G 4089 SMAD4 [′TATTGGTGTTCCATTGCTTACTTTGRAATG (p.Glu330Gly) GATGTTCAGGTAGGAGAGACA′] 4106 NM_020630.4(RET): c.1996A > G 5979 RET [′CCACTGCTACCACAAGTTTGCCCACVAGCC (p.Lys666Glu) ACCCATCTCCTCAGCTGAGAT′] 4107 NM_020630.4(RET): c.2342A > G 5979 RET [′CTGTCAGAGTTCAACGTCCTGAAGCRGGTC (p.Gln781Arg) AACCACCCACATGTCATCAAA′] 4108 NM_000060.3(BTD): c.194A > G 686 BTD [′TATTATGTGGCTGCCGTGTATGAGCRTCCA (p.His65Arg) TCCATCCTGAGTCTGAACCCT′] 4109 NM_000060.3(BTD): c.278A > G 686 BTD [′CTCATGAACCAGAACCTTGACATCTRTGAA (p.Tyr93Cys) CAGCAAGTGATGACTGCAGCC′] 4110 NM_000060.3(BTD): c.356A > G 686 BTD [′CCAGAAGATGGCATTCATGGATTCARCTTT (p.Asn119Ser) ACAAGAACATCCATTTATCCA′] 4111 NM_000060.3(BTD): c.364A > G 686 BTD [′TGGCATTCATGGATTCAACTTTACARGAAC (p.Arg122Gly) ATCCATTTATCCATTTTTGGA′] 4112 NM_000060.3(BTD): c.515A > G 686 BTD [′AGGGGAGATATGTTCTTGGTGGCCARTCTT (p.Asn172Ser) GGGACAAAGGAGCCTTGTCAT′] 4113 NM_000060.3(BTD): c.583A > G 686 BTD [′CCCAAAAGATGGGAGATACCAGTTCRACA (p.Asn195Asp) CAAATGTCGTGTTCAGCAATAA′] 4114 NM_000060.3(BTD): c.584A > G 686 BTD [′CCAAAAGATGGGAGATACCAGTTCARCAC (p.Asn195Ser) AAATGTCGTGTTCAGCAATAAT′] 4115 NM_000060.3(BTD): c.641A > G 686 BTD [′CTTGTTGACCGCTACCGTAAACACARCCTC (p.Asn214Ser) TACTTTGAGGCAGCATTCGAT′] 4116 NM_000060.3(BTD): c.880A > G 686 BTD [′GAAAGCTTTTGCTGTTGCCTTTGGCRTCAA (p.Ile294Val) CGTTCTGGCAGCTAATGTCCA′] 4117 NM_000060.3(BTD): c.1205A > G 686 BTD [′TTTCACTCTGAGATGATGTATGACARTTTC (p.Asn402Ser) ACCCTGGTCCCTGTCTGGGGA′] 4118 NM_000060.3(BTD): c.1313A > G 686 BTD [′AGGCCCACCTTATCCAAAGAGCTGTRTGCC (p.Tyr438Cys) CTGGGGGTCTTTGATGGGCTT′] 4119 NM_000060.3(BTD): c.1619A > G 686 BTD [′ACGGCGGCTCTCTATGGGCGCTTGTRTGAG (p.Tyr540Cys) AGGGACTAGGAAAAGTGTGTG] 4120 NM_000155.3(GALT): c.1A > G 2592 GALT [′TTCCAGCGGATCCCCCGGTGGCCTCRTGTC (p.Met1Val) GCGCAGTGGAACCGATCCTCA′] 4121 NM_000155.3(GALT): c.67A > G 2592 GALT [′GTCAGAGGCGGACGCCGCAGCAGCARCCT (p.Thr23Ala) TCCGGGCAAACGGTAACTGCAC′] 4122 NM_000155.3(GALT): c.253 − 2A > G 2592 GALT [′GTGCTTCTAGCCTATCCTTGTCGGTRGGTG AATCCCCAGTACGATAGCACC′] 4123 NM_000155.3(GALT): c.290A > G 2592 GALT [′TACGATAGCACCTTCCTGTTTGACARCGAC (p.Asn97Ser) TTCCCAGCTCTGCAGCCTGAT′] 4124 NM_000155.3(GALT): c.308A > G 2592 GALT [′TTTGACAACGACTTCCCAGCTCTGCRGCCT (p.Gln103Arg) GATGCCCCCAGTCCAGGTAAC′] 4125 NM_000155.3(GALT): c.379A > G 2592 GALT [′ATCTTTTCTCCCGTCACCACCCAGTRAGGT (p.Lys127Glu) CATGTGCTTCCACCCCTGGTC′] 4126 NM_000155.3(GALT): c.424A > G 2592 GALT [′CTGGTCGGATGTAACGCTGCCACTCRTGTC (p.Met142Val) GGTCCCTGAGATCCGGGCTGT′] 4127 NM_000155.3(GALT): c.565 − 2A > G 2592 GALT [′TTCTTCTCTGCTTTTGCCCCTTGACRGGTAT GGGCCAGCAGTTTCCTGCCA′] 4128 NM_000155.3(GALT): c.574A > G 2592 GALT [′TTTTGCCCCTTGACAGGTATGGGCCRGCAG (p.Ser192Gly) TTTCCTGCCAGATATTGCCCA′] 4129 NM_000155.3(GALT): c.626A > G 2592 GALT [′CGTGAGGAGCGATCTCAGCAGGCCTVTAA (p.Tyr209Cys) GAGTCAGCATGGAGAGCCCCTG′] 4130 NM_000155.3(GALT): c.752A > G 2592 GALT [′GTCCCCTTCTGGGCAACATGGCCCTVCCAG (p.Tyr251Cys) ACACTGCTGCTGCCCGTCGGC′] 4131 NM_000155.3(GALT): c.812A > G 2592 GALT [′CGGCTACCTGAGCTGACCCCTGCTGRGCGT (p.Glu271Gly) GATGGTCAGTCTCCCAAGTAG′] 4132 NM_000155.3(GALT): c.821 − 2A > G 2592 GALT [′AGGCTGAGAGTCAGGCTCTGATTCCRGAT CTAGCCTCCATCATGAAGAAGC′] 4133 NM_000155.3(GALT): c.854A > G 2592 GALT [′TCCATCATGAAGAAGCTCTTGACCARGTAT (p.Lys285Arg) GACAACCTCTTTGAGACGTCC′] 4134 NM_000155.3(GALT): c.968A > G 2592 GALT [′CATTGGCAGCTGCACGCTCATTACTRCCCT (p.Tyr323Cys) CCGCTCCTGCGCTCTGCCACT′] 4135 NM_000155.3(GALT): c.1001A > G 2592 GALT [′CTCCTGCGCTCTGCCACTGTCCGGARATTC (p.Lys334Arg) ATGGTTGGCTACGAAATGCTT′] 4136 NM_000155.3(GALT): c.1048A > G 2592 GALT [′GCTTGCTCAGGCTCAGAGGGACCTCRCCCC (p.Thr350Ala) TGAGCAGGTCAGGACTCAGAA′] 4137 NM_000155.3(GALT): c.1132A > G 2592 GALT [′GCAGAAGGACAGGGAGACAGCAACCRTCG (p.Ile378Val) CCTGACCACGCCGACCACAGGG′] 4138 NM_000553.4(WRN): c.403A > G 7486 WRN [′GTTGCTTGAAAATAAAGCAGTTAAARAGG (p.Lys135Glu) CAGGTGTAGGAATTGAAGGAGA′] 4139 NM_000433.3(NCF2): c.481A > G 4688 NCF2 [′CTTACCCAGACACACTCCATCGCCTYGTCG (p.Lys161Glu) ATTTTGGAATGTCTGGGCTCA′] 4140 NM_198056.2(SCN5A): c.1673A > G 6331 SCN5A [′ACAGCGGGGGAGAGCGAGAGCCACCRCAC (p.His558Arg) ATCACTGCTGGTGCCCTGGCCC′] 4141 NM_000074.2(CD40LG): c.386A > 959 CD40LG [′CAAATTGCGGCACATGTCATAAGTGRGGC G (p.Glu129Gly) CAGCAGTAAAACAACATCTGGT′] 4142 NM_002863.4(PYGL): c.1016A > G 5836 PYGL [′CCCACCCAGGTGGCCATCCAGCTGARTGA (p.Asn339Ser) CACTCACCCTGCACTCGCGATC′] 4143 NM_000142.4(FGFR3): c.1454A > G 2261 FGFR3 [′CCCCTTGGGGAGGGCTGCTTCGGCCRGGT (p.Gln485Arg) GGTCATGGCGGAGGCCATCGGC′] 4144 NM_001101.3(ACTB): c.34A > G 60 ACTB [′TGATATCGCCGCGCTCGTCGTCGACVACG (p.Asn12Asp) GCTCCGGCATGTGCAAGGCCGG′] 4145 NM_001202.3(BMP4): c.362A > G 652 BMP4 [′TGGGGGAAGAGACTGACCTTCGTGGYGGA (p.His121Arg) AGCTCCTCACGGTGTTGGCCCG′] 4146 NM_000094.3(COL7A1): c.425A > 1294 COL7A1 [′CAGCTGGCCCGACCTGGTGTCCCCARGGT G (p.Lys142Arg) GATCCCTACCCCTACCATGCCT′] NM_001130823.1(DNMT1): c.1532A > 1786 DNMT1 [] G (p.Tyr511Cys) 4147 NM_000138.4(FBN1): c.5096A > G 2200 FBN1 [′AGAAGAAGTTTGTGCTACAGAAACTRCTA (p.Tyr1699Cys) TGCTGACAACCAGACCTGTGAT] 4148 NM_000138.4(FBN1): c.5087A > G 2200 FBN1 [′CTAGATATGAGAAGAAGTTTGTGCTRCAG (p.Tyr1696Cys) AAACTACTATGCTGACAACCAG′] 4149 NM_000138.4(FBN1): c.5099A > G 2200 FBN1 [′AGAAGTTTGTGCTACAGAAACTACTRTGCT (p.Tyr1700Cys) GACAACCAGACCTGTGATGGA′] 4150 NM_001244710.1(GFPT1): c.43A > 2673 GFPT1 [′TTACTTAAACTACCATGTTCCTCGARCGAG G (p.Thrl5Ala) ACGAGAAATCCTGGAGACCCT′] 4151 NM_002292.3(LAMB2): c.440A > G 3913 LAMB2 [′CTGGAGGCTGAGTTTCATTTCACACRCCTC (p.His147Arg) ATTATGACCTTCAAGGTGCCT′] 4152 NM_005211.3(CSF1R): c.1754 − 1436 CSF1R [′GACTTAAGGGACCTGTGTGCGTGGCRGGT 2A > G AAGACCCTCGGAGCTGGAGCCT′] 4153 NM_005247.2(FGF3): c.146A > G 2248 FGF3 [′GGGGCGCCCCGGCGCCGCAAGCTCTRCTG (p.Tyr49Cys) CGCCACGAAGTACCACCTCCAG′] 4154 NM_005247.2(FGF3): c.317A > G 2248 FGF3 [′GCCATGAACAAGAGGGGACGACTCTRTGC (p.Tyr106Cys) TTCGGTGAGTCCAGGCTGTCAC′] 4155 NM_005188.3(CBL): c.1112A > G 867 CBL [′TAATCAAAGGAACAATATGAATTATRCTG (p.Tyr371Cys) TGAGATGGGCTCCACATTCCAA′] 4156 NM_000313.3(PROS1): c.701A > G 5627 PROS1 [′TGTGAATGCCCCGAAGGCTACAGATRTAA (p.Tyr234Cys) TCTCAAATCAAAGTCTTGTGAA′] 4157 NM_000329.2(RPE65): c.1292A > G 6121 RPE65 [′TACCAGAAGTATTGTGGGAAACCTTRCAC (p.Tyr431Cys) ATATGCGTATGGACTTGGCTTG′] 4158 NM_001040142.1(SCN2A): c.4419A > 6326 SCN2A [′TGAATCTTTTCATTGGTGTCATCATRGATA G (p.Ile1473Met) ACTTCAACCAACAGAAAAAGA′] 4159 NM_001040142.1(SCN2A): c.754A > 6326 SCN2A [′GTCAGTGAAGAAGCTTTCTGATGTCRTGAT G (p.Met252Val) CTTGACTGTGTTCTGTCTAAG′] 4160 NM_004612.3(TGFBR1): c.134A > 7046 TGFBR1 [′TTCTGCCACCTCTGTACAAAAGACARTTTT G (p.Asn45Ser) ACTTGTGTGACAGATGGGCTC′] 4161 NM_003688.3(CASK): c.2168A > G 8573 CASK [′TTTGATCAATTAGATCTTGTCACATRTGAA (p.Tyr723Cys) GAAGTAGTAAAACTGCCAGCA′] 4162 NM_015884.3(MBTPS2): c.1523A > 51360 MBTPS2 [′GGTGGCAGTGTACTTTTGGCTGCCARTGTG G (p.Asn508Ser) ACCCTGGGACTCTGGATGGTT′] 4163 m.10450A > G 4573 MT-TR [′ACGAATGATTTCGACTCATTAAATTRTGAT AATCATATTTACCAAATGCCC′] 4164 m.5816A > G 4511 MT-TC [′GAATTTGCAATTCAATATGAAAATCRCCTC GGAGCTGGTAAAAAGAGGCCT′] 4165 m.608A > G 4558 MT-TF [′GTAGCTTACCTCCTCAAAGCAATACRCTGA AAATGTTTAGACGGGCTCACA′] 4166 NM_172337.2(OTX2): c.674A > G 5015 OTX2 [′CTGATTGAGATGGCTGGTGACTGCAYTGG (p.Asn225Ser) TACCCATGGGACTGAGTGTGGC′] 4167 NM_001376.4(DYNC1H1): c.917A > 1778 DYNC1H1 [′CTCCTGACTCTGGATATCTTGAAACRTGGC G (p.His306Arg) AAGCGCTTCCATGCCACCGTC′] 4168 NM_001376.4(DYNC1H1): c.2011A > 1778 DYNC1H1 [′GAAGCGGGTGGAAGATGTCCTTGGCRAGG G (p.Lys671Glu) GCTGGGAGAATCACGTGGAGGG′] 4169 NM_001376.4(DYNC1H1): c.2909A > 1778 DYNC1H1 [′CTAAGAATAACCAATCAGGTAATCTRCTTG G (p.Tyr970Cys) AATCCACCAATTGAAGAGTGC′] 4170 NM_004380.2(CREBBP): c.2728A > 1387 CREBBP [′ACTGTAGGGGTGCTCTGGGTTTGGGHAGC G (p.Thr910Ala) ACTGGGCACTGAGCCAGGAGTC′] 4171 NM_000459.4(TEK): c.2690A > G 7010 TEK [′ATGCTCTCTTCCTTCCCTCCAGGCTVCTTGT (p.Tyr897Cys) ACCTGGCCATTGAGTACGCG] 4172 NM_001354.5(AKR1C2): c.235A > 1646 AKR1C2 [′AGATGGCAGTGTGAAGAGAGAAGACRTAT G (p.Ile79Val) TCTACACTTCAAAGGTACTGTG′] 4173 NM_007315.3(STAT1): c.604A > G 6772 STAT1 [′ACAAGAACAGCTGTTACTCAAGAAGRTGT (p.Met202Val) ATTTAATGCTTGACAATAAGAG′] 4174 NM_007315.3(STAT1): c.494A > G 6772 STAT1 [′GAGCATGAAATCAAGAGCCTGGAAGRTTT (p.Asp165Gly) ACAAGATGAATATGACTTCAAA′] 4175 NM_007315.3(STAT1): c.862A > G 6772 STAT1 [′GTTGGAGGAATTGGAACAGAAATACRCCT (p.Thr288Ala) ACGAACATGACCCTATCACAAA′] 4176 NM_002052.4(GATA4): c.928A > G 2626 GATA4 [′CCTCCAGGTCCCCAGGCCTCTTGCARTGCG (p.Met310Val) GAAAGAGGGGATCCAAACCAG′] 4177 NM_014191.3(SCN8A): c.5302A > G 6334 SCN8A [′CATGTACATTGCCATCATCCTGGAGRACTT (p.Asn1768Asp) CAGTGTAGCCACAGAGGAAAG′] 4178 NM_000209.3(PDX1): c.533A > G 3651 PDX1 [′TACATCTCACGGCCGCGCCGGGTGGRGCT (p.Glu178Gly) GGCTGTCATGTTGAACTTGACC′] 4179 NM_000890.3(KCNJ5): c.472A > G 3762 KCNJ5 [′CATTGGGTATGGCTTCCGAGTCATCRCAGA (p.Thr158Ala) GAAGTGTCCAGAGGGGATTAT′] 4180 NM_000256.3(MYBPC3): c.2234A 4607 MYBPC3 [′ACGGTCGAGGGGGCAGAGAAGGAAGRTG > G (p.Asp745Gly) AGGGCGTCTACACGGTCACAGTG′] 4181 NM_005359.5(SMAD4): c.1498A > 4089 SMAD4 [′TGTTGATGACCTTCGTCGCTTATGCRTACT G (p.Ile500Val) CAGGATGAGTTTTGTGAAAGG′] 4182 NM_005359.5(SMAD4): c.1500A > 4089 SMAD4 [′TTGATGACCTTCGTCGCTTATGCATRCTCA G (p.Ile500Met) GGATGAGTTTTGTGAAAGGCT′] 4183 NM_001184.3(ATR): c.6431A > G 545 ATR [′TATCAATTTTTGACTGCTTTTTCACRATTGA (p.Gln2144Arg) TCTCTCGAATTTGTCATTCT′] 4184 NM_153427.2(PITX2): c.262A > G 5308 PITX2 [′CCCCAACCGCCCCCAGGTTTGGTTCRAGAA (p.Lys88Glu) TCGTCGGGCCAAATGGAGAAA′] 4185 NM_005257.5(GATA6): c.1354A > 2627 GATA6 [′CTGTGCCAACTGTCACACCACAACTRCCAC G (p.Thr452Ala) CTTATGGCGCAGAAACGCCGA′] 4186 NM_005257.5(GATA6): c.1396A > 2627 GATA6 [′AAACGCCGAGGGTGAACCCGTGTGCVATG G (p.Asn466Asp) CTTGTGGACTCTACATGAAACT′] 4187 NM_000414.3(HSD17B4): c.650A > 3295 HSD17B4 [′CTTGTGGAAGCCCTGAAGCCAGAGTRTGT G (p.Tyr217Cys) GGCACCTCTTGTCCTTTGGCTT′] 4188 NM_004153.3(ORC1): c.380A > G 4998 ORC1 [′GCCTGTGACAGCAACATTAATGCGGRGAC (p.Glu127Gly) CATCATTGGCCTTGTTCGGGTA′] 4189 NM_002552.4(ORC4): c.521A > G 5000 ORC4 [′CATCATAAAAACCAAACACTTCTCTRTAAT (p.Tyr174Cys) CTTTTTGACATTTCTCAGTCT′] 4190 NM_004656.3(BAP1): c.2057 − 8314 BAP1 [′GCTCCACTAGGTTGGCCAGCATGCCYGCG 2A > G AAGAGGTAGAGACCCTTGAGCA′] 4191 NM_004656.3(BAP1): c.438 − 2A > G 8314 BAP1 [′TCAGGGAGGTGGCGTGGCTCGGGCCYGGG GAAAAACAGAGTCAGGGCCCAA′] NM_003590.4(CUL3): c.1238A > G 8452 CUL3 [] (p.Asp413Gly) 4192 NM_004813.2(PEX16): c.992A > G 9409 PEX16 [′TACTTGCCCACCTGGCAGAAAATCTRCTTC (p.Tyr331Cys) TACAGTTGGGGCTGACAGACC′] 4193 NM_004544.3(NDUFA10): c.1A > G 4705 NDUFA10 [′TTGATCCTGAGCTGACCGGGTAGCCRTGGC (p.Met1Val) CTTGCGGCTCCTGAAGCTGGC′] 4194 NM_004544.3(NDUFA10): c.425A > 4705 NDUFA10 [′TGGTTGTACAGCAGTCGCCTGCTGCRGTAC G (p.Gln142Arg) TCAGATGCCTTGGAGCACTTG′] 4195 NM_006796.2(AFG3L2): c.1996A > 10939 AFG3L2 [′TATTTTTCAGATTGTTCAGTTTGGCDTGAA G (p.Met666Val) TGAAAAGGTTGGGCAAATCTC′] 4196 NM_006796.2(AFG3L2): c.1847A > 10939 AFG3L2 [′TATTTACCAAAAGAACAATACCTCTRTACC G (p.Tyr616Cys) AAAGAGCAGCTCTTGGATAGG′] 4197 NM_001199397.1(NEK1): c.869 − 4750 NEK1 [′CAAAATTCTTTGTATCTTTTCATCTRGCTA 2A > G AAAGACCAGCTTCAGGACAAA′] 4198 NM_006587.3(CORIN): c.949A > G 10699 CORIN [′GAATCTGTTTCACTGTCACACAGGCRAGTG (p.Lys317Glu) CCTTAATTACAGCCTTGTGTG′] 4199 NM_006587.3(CORIN): c.1414A > G 10699 CORIN [′CATGAATTTGCCCTACAACAGTACARGTTA (p.Ser472Gly) TCCAAATTATTTTGGCCACAG′] 4200 NM_015560.2(OPA1): c.1294A > G 4976 OPA1 [′AGCTTACATGCAGAATCCTAATGCCRTCAT (p.Ile432Val) ACTGTGTATTCAAGGTAAATC′] 4201 NM_021625.4(TRPV4): c.1805A > G 59341 TRPV4 [′CGTGGGCTGAAGCTGACGGGGACCTRTAG (p.Tyr602Cys) CATCATGATCCAGAAGGTACGG′] 4202 NM_021625.4(TRPV4): c.590A > G 59341 TRPV4 [′TCTACGGGGAAGACCTGCCTGCCCARGGC (p.Lys197Arg) CTTGCTGAACCTGAGCAATGGC′] 4203 NM_021625.4(TRPV4): c.826A > G 59341 TRPV4 [′GGCCCGTGGGCGCTTCTTCCAGCCCRAGG (p.Lys276Glu) ATGAGGGGGGCTACTTCTACTT′] 4204 NM_024022.2(TMPRSS3): c.308A > 64699 TMPRSS3 [′GTCTCGGATTGCAAAGACGGGGAGGRCGA G (p.Asp103Gly) GTACCGCTGTGGTAAGGTCATG′] 4205 NM_016599.4(MYOZ2): c.738A > G 51778 MYOZ2 [′GATGGATATCTGAGAATATTCCTATRGTGA (p.Ile246Met) TAACAACCGAACCTACAGATG′] NM_017415.2(KLHL3): c.1670A > G 26249 KLHL3 [] (p.Tyr557Cys) 4206 NM_019109.4(ALG1): c.1129A > G 56052 ALG1 [′GTCCTCCAGTGGCCTGGACCTGCCCRTGAA (p.Met377Val) GGTGGTGGACATGTTCGGGTG′] 4207 NM_031885.3(BBS2): c.472 − 2A > G 583 BBS2 [′GCTAATGGTTTGGGGTTTTATTTTCRGGTT ACTGGAGACAATGTTAATTCC′] 4208 NM_006886.3(ATP5E): c.35A > G −1 — [′TACCTTCTCTCATTTTCTCCCAGCTRCATCC (p.Tyr12Cys) GATACTCCCAGATCTGTGCA′] 4209 NM_022445.3(TPK1): c.656A > G 27010 TPK1 [′CACAACACCAGACCCGTCGTAGGTAYTGG (p.Asn219Ser) AAGTACTGACCAATGTTCCAAA′] 4210 NM_000017.3(ACADS): c.1031A > 35 ACADS [′CTCTGACTGTACCCCCATGTTTAGGRGGCA G (p.Glu344Gly) GCCATGGCCAAGCTGGCCGCC′] 4211 NM_016013.3(NDUFAF1): c.758A > 51103 NDUFAF1 [′GGGGGACCCTACTGGCAGGAGGTCARGGT G (p.Lys253Arg) AACAGCATAAATCTTCATTGTT′] 4212 NM_032580.3(HES7): c.172A > G 84667 HES7 [′GAACCCGAAGCTGGAGAAAGCGGAGRTAT (p.Ile58Val) TGGAGTTCGCCGTGGGCTACTT′] 4213 NM_024887.3(DHDDS): c.124A > G 79947 DHDDS [′GGACGGGAACCGTCGCTATGCCAAGRAGT (p.Lys42Glu) GCCAGGTGGAGCGGCAGGAAGG′] 4214 NM_024700.3(SNEP1): c.1097A > G 79753 SNIP1 [′CTCAAATTTGGATTCAGTAGCAGAGRATA (p.Glu366Gly) CGTCTTGCTCCATGAGTCGTCG′] 4215 NM_206933.2(USH2A): c.7595 − 7399 USH2A [′AATTGAACACCTCTCCTTTCCCAAGRTAAG 2144A > G AGATCATCTTTAAGAAAAGGC′] 4216 NM_016952.4(CDON): c.2368A > G 50937 CDON [′GTTTTTGTTTTCCCTCAAAGGTTCARCATA (p.Thr790Ala) CAAATTTAGGGTCATTGCCAT′] 4217 NM_020435.3(GJC2): c.-167A > G 57165 GJC2 [′AGACCCTGAGGCCGAGGGGGGAACARTGG GGCCCTTGAGGGCCCCTCCTCC′] 4218 NM_024549.5(TCTN1): c.221 − 79600 TCTN1 [′TGGATCCTACCCCTCTTTTTTCTGCRGTTGC 2A > G TGTTCTCTGTGTCTGTGACT′] 4219 NM_031427.3(DNAL1): c.449A > G 83544 DNAL1 [′CTCGAAGACCTGGTGTTTGTAGGCARTCCC (p.Asn150Ser) TTGGAAGAGAAACATTCTGCT′] 4220 NM_145046.4(CALR3): c.245A > G 125972 CALR3 [′AGTTTTCCCTTTATTGCTGAACGGTYTGAA (p.Lys82Arg) GCGTGCAGAGATGGCATAGAA′] 4221 NM_020320.3(RARS2): c.1024A > G 57038 RARS2 [′AATGGACAAGTATAATTTTGATACARTGAT (p.Met342Val) ATATGTGGTAAGTAATCAGAA′] 4222 NM_020320.3(RARS2): c.35A > G 57038 RARS2 [′CTCTGCGCGCTCCGGGATCCATACCYGGC (p.Gln12Arg) AAGCAATAGCGCGGCGAAAGCC′] 4223 NM_001017361.2(KHDC3L): c.1A > 154288 KHDC3L [′TGTCTCCTGCAGGACCGGCCGCAGCRTGG G (p.MetlVal) ACGCTCCCAGGCGGTTTCCGAC′] 4224 NM_024753.4(TTC21B): c.2758 − 79809 TTC21B [′TAATCGTGCCAGTTCCAACATAATCYGTAG 2A > G AGCAAAGGGCTAGATTCATCA′] 4225 NM_001145901.1(SARS2): c.1175A > 54938 SARS2 [′TTCGTCCTTGACCGCAGGGTCCTGGRTATG G (p.Asp392Gly) CCCACCCAAGAACTGGGCCTC′] 4226 NM_000552.3(VWF): c.3437A > G 7450 VWF [′AACGGGTATGAGTGTGAGTGGCGCTRTAA (p.Tyr1146Cys) CAGCTGTGCACCTGCCTGTCAA′] 4227 NM_014985.3(CEP152): c.2000A > 22995 CEP152 [′ACCTTACCTATCCACAGCTTCTTGTYTGTC G (p.Lys667Arg) ATGGTCAAAATCTTGTACCAT′] 4228 NM_024809.4(TCTN2): c.1506 − 79867 TCTN2 [′GCTGAGAAATGTCTTACTCTCTTGCRGGGA 2A > G GAATGCTGTTGAAAGACTTGA′] 4229 NM_198994.2(TGM6): c.980A > G 343641 TGM6 [′CGGACCCTGGAGGACCTGACAGAAGRCAG (p.Asp327Gly) CATGTGGTGGGTCCTGCCCCCA′] 4230 NM_052873.2(IFT43): c.1A > G 112752 IFT43 [′GAAGTGACGTCAGGCGGCCGCGGAGRTGG (p.Met1Val) AGGATTTGCTCGACTTGGACGA′] 4231 NM_201269.2(ZNF644): c.2014A > 84146 ZNF644 [′AACATTTGGATCAACCTCACAATCARGTA G (p.Ser672Gly) GTTTTTCAAAAATTCATAAGCG′] 4232 NM_201269.2(ZNF644): c.1759A > 84146 ZNF644 [′GTAGTAAAAGGACACATCTTACATAYGTA G (p.Ile587Val) GGTAGCTGATTTTTTGGATGAT′] 4233 NM_018699.3(PRDM5): c.320A > G 11107 PRDM5 [′TTTCAGGAAGGAGAAAACATTTTCTRTTTG (p.Tyr107Cys) GCAGTTGAAGATATAGAAACA′] 4234 NM_001198799.2(ASCC1): c.953A > 51008 ASCC1 [′CTATAGTTCTCTGGGGGACTTACCAYTGGG G (p.Asn318Ser) GTCTTTCCTGAATAGTGTATT′] 4235 NM_007055.3(POLR3A): c.2554A > 11128 POLR3A [′ACCAACTGAGTTTTTCTTCCACACARTGGC G (p.Met852Val) CGGCCGGGAAGGTCTAGTCGA′] 4236 NM_001256047.1(C19orf12): c.391A > 83636 C19orf12 [′TACTGGATCTCGGCCCGCAGCTCCTYGGTG G (p.Lys131Glu) ACGTAGTTCACCAGCATGGCC′] 4237 NM_016464.4(TMEM138): c.287A > 51524 TMEM138 [′TACTTTGCCCTCAGCATCTCCCTTCRTGTCT G (p.His96Arg) GGGTCATGGTAAGAGTGGCA′] 4238 NM_016464.4(TMEM138): c.389A > 51524 TMEM138 [′TTCCTCCCCACAGCAGCAGTGTTGTRCTGC G (p.Tyr130Cys) TACTTCTATAAACGGACAGCC′] 4239 NM_177965.3(C8orf37): c.545A > G 157657 C8orf37 [′AAAGGAACACGGGCATATGCCTGCCRGTG (p.Gln182Arg) TAGCTGGAGAACTATTGAAGAA′] 4240 NM_001004334.3(GPR179): c.659A > 440435 GPR179 [′CCCAAGTGGCCGCAGGCAGATGGATRTGT G (p.Tyr220Cys) GGGGGACACGCAGCAGGTGAGG′] 4241 NM_004963.3(GUCY2C): c.1160A > 2984 GUCY2C [′AGCAGTGAGACATACTTTCTTGGTGYCCAC G (p.Asp387Gly) AGAGGTATACAGAAGCACCAT′] 4242 NM_024110.4(CARD14): c.425A > 79092 CARD14 [′AGCCTGCAGGAGGAGCTGAACCAGGRAAA G (p.Glu142Gly) GGGGCAGAAGGAGGTGCTGCTG′] 4243 NM_018105.2(THAP1): c.70A > G 55145 THAP1 [′CAAGGACAAGCCCGTTTCTTTCCACRAGTG (p.Lys24Glu) AGGACCCTGCGCGCCTCGCGG′] 4244 NM_000933.3(PLCB4): c.1868A > G 5332 PLCB4 [′AACAAACGGCAAATGAGTCGCATTTRCCC (p.Tyr623Cys) CAAGGGAGGCCGAGTCGATTCC′] 4245 NM_001172646.1(PLCB4): c.986A > 5332 PLCB4 [′CACTACTTCATCAGTTCTTCCCATAVCACT G (p.Asn329Ser) TATCTCACTGGCAGACAGTTC′] 4246 NM_005850.4(SF3B4): c.1A > G 10262 SF3B4 [′GGGAGACGGCGGGATCTCTTTCGCCRTGG (p.Met1Val) CTGCCGGGCCGATCTCCGAGCG′] 4247 NM_014714.3(IFT140): c.932A > G 9742 IFT140 [′TGGGACATAGAACGAGGAGAGAATTRTAT (p.Tyr311Cys) ACTGAGTCCAGATGAGAAGTTT′] 4248 NM_000258.2(MYL3): c.517A > G 4634 MYL3 [′GACAGAAGACGAAGTGGAGAAGTTGRTGG (p.Met173Val) CTGGGCAAGAGGACTCCAATGG′] 4249 NM_032578.3(MYPN): c.59A > G 84665 MYPN [′ATATCTCAGCTTCTAAGAGAGAGCTRTTTA (p.Tyr20Cys) GCTGAAACCAGACATCGGGGA′] 4250 NM_001018005.1(TPM1): c.742A > 7168 TPM1 [′TGAGTTTGCGGAGAGGTCAGTAACTRAAT G (p.Lys248Glu) TGGAGAAAAGCATTGATGACTT′] 4251 NM_005006.6(NDUFS1): c.1783A > 4719 NDUFS1 [′AGCTGCTTACACAGAGAAGTCTGCTRCAT G (p.Thr595Ala) ATGTCAACACTGAGGGTAGAGC′] 4252 NM_033360.3(KRAS): c.439A > G 3845 KRAS [′AATTCCTTTTATTGAAACATCAGCARAGAC (p.Lys147Glu) AAGACAGGTAAGTAACACTGA′] 4253 NM_014236.3(GNPAT): c.1556A > 8443 GNPAT [′GAAGATGAACTCATCTGCAAAAACAHCAC G (p.Asp519Gly) GTAGGAAGCGAAAGCAACTGTA′] 4254 NM_017565.3(FAM20A): c.813 − −1 — [′GGCACCCGTCGGAAGTCCAGAATCCYGCA 2A > G AGAGAGGAAGCTCTGTTCCATC′] 4255 NM_017565.3(FAM20A): c.590 − 54757 FAM20A [′AGGCTTTCTCATCTTGACTGTAATCYGCAA 2A > G AGGAGGAGAAGGGCAATGAGA′] 4256 NM_017636.3(TRPM4): c.2741A > 54795 TRPM4 [′CTGCTTCACATCTTCACGGTCAACARACAG G (p.Lys914Arg) CTGGGGCCCAAGATCGTCATC′] 4257 NM_000076.2(CDKN1C): c.832A > 1028 CDKN1C [′GCTGTCGCCCGCAGATTTCTTCGCCRAGCG G (p.Lys278Glu) CAAGAGATCAGCGCCTGAGAA′] 4258 NM_022912.2(REEP1): c.304 − 65055 REEP1 [′AGTGTGCCTCTGTTTTTCCTTTGACRGGAA 2A > G ATCGATGATTGTCTGGTCCAA′] 4259 NM_000053.3(ATP7B): c.2305A > G 540 ATP7B [′TGTGACATTCTTCGACACGCCCCCCRTGCT (p.Met769Val) CTTTGTGTTCATTGCCCTGGG′] 4260 NM_000053.3(ATP7B): c.3620A > G 540 ATP7B [′GTCCACACCCATGCTCTGCAGCGTGYGCA (p.His1207Arg) CAGCCAGGGCAGCCTCCTGCTT′] 4261 NM_000492.3(CFTR): c.3140 − 1080 CFTR [′ACATTTTGTGTTTATGTTATTTGCARTGTTT 26A > G TCTATGGAAATATTTCACAG′] 4262 NM_000570.4(FCGR3B): c.194A > 2215 FCGR3B [′GCTCGAGGCCTGGCTTGAGATGAGGYTCT G (p.Asn65Ser) CATTGTGAAACCACTGTGTGGA′] 4263 NM_198253.2(TERT): c.2705A > G 7015 TERT [′TATGGCTGCGTGGTGAACTTGCGGARGAC (p.Lys902Arg) AGTGGTGAACTTCCCTGTAGAA′] 4264 NM_005022.3(PFN1): c.350A > G 5216 PFN1 [′GTTGATCAAACCACCGTGGACACCTYCTTT (p.Glu117Gly) GCCCATCAGCAGGACTAGCGC′] 4265 NM_005349.3(RBPJ): c.188A > G 3516 RBPJ [′GTTGCACAGAAGTCATATGGAAATGRAAA (p.Glu63Gly) AAGGTAAGATTATTTTTCTGGT′] 4266 NM_005349.3(RBPJ): c.505A > G 3516 RBPJ [′GATAAAAGTCATCTCCAAACCTTCCRAAA (p.Lys169Glu) AGAAGCAGTCATTGAAAAATGC′] 4267 NM_022787.3(NMNAT1): c.817A > 64802 NMNAT1 [′GGTCATCCTGGCCCCTTTGCAGAGARACAC G (p.Asn273Asp) TGCAGAAGCTAAGACATAGGA′] 4268 NM_020921.3(NIN): c.3665A > G 51199 NIN [′CACAGAAACATCAAAAAGTAGGTCCYGTT (p.Gln1222Arg) TCTTTTCAGAAGCTCGATCACA′] 4269 NM_020921.3(NIN): c.5126A > G 51199 NIN [′AAAATCTCTAGTGTTCTAAGCTACARCGAA (p.Asn1709Ser) AAACTGCTGAAAGAAAAGGAA′] 4270 NM_005340.6(HINT1): c.152A > G 3094 HINT1 [′GACATTTCCCCTCAAGCACCAACACRTTTT (p.His51Arg) CTGGTGATACCCAAGAAACAT′] 4271 NM_005050.3(ABCD4): c.956A > G 5826 ABCD4 [′GAGCTGGGTGAAGCAGCTGATGAGGYAGA (p.Tyr319Cys) TGCACACAAAGGCATTCTGGAC′] 4272 NM_000155.3(GALT): c.950A > G 2592 GALT [′GCTGGGGCCAACTGGAACCATTGGCRGCT (p.Gln317Arg) GCACGCTCATTACTACCCTCCG′] 4273 NM_007294.3(BRCA1): c.213 − 672 BRCA1 [′ACTTGCTGAGTGTGTTTCTCAAACARTTTA 12A > G ATTTCAGGAGCCTACAAGAAA′] 4274 NM_007294.3(BRCA1): c.4096 + 3A > 672 BRCA1 [′GCAAAGCATGGATTCAAACTTAGGTRTTG G GAACCAGGTTTTTGTGTTTGCC′] 4275 NM_007294.3(BRCA1): c.5194 −  72 BRCA1 [′TGATGGGTTGTGTTTGGTTTCTTTCVGCAT 2A > G GATTTTGAAGTCAGAGGAGAT′] 4276 NM_007294.3(BRCA1): c.5453A > 672 BRCA1 [′GTGCAGCCAGATGCCTGGACAGAGGRCAA G (p.Asp1818Gly) TGGCTTCCATGGTAAGGTGCCT′] 4277 NM_000059.3(BRCA2): c.1799A > 675 BRCA2 [′TATGCTATACATGATGAAACATCTTRTAAA G (p.Tyr600Cys) GGAAAAAAAATACCGAAAGAC′] 4278 NM_000059.3(BRCA2): c.476 − 675 BRCA2 [′ACAATTTTCCCCTTTTTTTACCCCCRGTGGT 2A > G ATGTGGGAGTTTGTTTCATA′] 4279 NM_000059.3(BRCA2): c.8168A > 675 BRCA2 [′AAAGTGGCCATTATTGAACTTACAGNTGG G (p.Asp2723Gly) GTGGTATGCTGTTAAGGCCCAG′] 4280 NM_000059.3(BRCA2): c.8633 − 675 BRCA2 [′GAATTAATAATCCTTTTGTTTTCTTDGAAA 2A > G ACACAACAAAACCATATTTAC′] 4281 NM_000060.3(BTD): c.629A > G 686 BTD [′AATAATGGAACCCTTGTTGACCGCTRCCGT (p.Tyr210Cys) AAACACAACCTCTACTTTGAG] 4282 NM_000060.3(BTD): c.968A > G 686 BTD [′ACCCCTCTGGAGTCCTTTTGGTACCRTGAC (p.His323Arg) ATGGAAAATCCCAAAAGTCAC′] 4283 NM_000155.3(GALT): c.857A > G 2592 GALT [′ATCATGAAGAAGCTCTTGACCAAGTRTGA (p.Tyr286Cys) CAACCTCTTTGAGACGTCCTTT′] 4284 NM_000060.3(BTD): c.128A > G 686 BTD [′TGTTACGTGGTTGCCCTGGGAGCCCRCACC (p.His43Arg) GGGGAGGAGAGCGTGGCTGAC′] 4285 NM_002437.4(MPV17): c.262A > G 4358 MPV17 [′CACCACCAAAGTGGATGCACTGAAGRAGA (p.Lys88Glu) TGTTGTTGGATCAGGTGAGCAG′] 4286 NM_002769.4(PRSS1): c.65A > G −1 — [′GTTGCTGCCCCCTTTGATGATGATGRCAAG (p.Asp22Gly) ATCGTTGGGGGCTACAACTGT′] 4287 NM_005211.3(CSF1R): c.2320 − 1436 CSF1R [′GACTAACCCTGCAGTGCTTTCCCTCRGTGC 2A > G ATCCACCGGGACGTGGCAGCG′] 4288 NM_024312.4(GNPTAB): c.118 − 79158 GNPTAB [′GAAAACGTTTCTTTTTCTTTGTTCTRGGTG 2A > G GTTCTGGAATGGAGCCGAGAT′] 4289 NM_000495.4(COL4A5): c.3107 − 1287 COL4A5 [′AAATATTATATATCACATATTTTCARCAGG 4A > G GCCTCAGGGTGTGGAAGGGCC′] 4290 NM_000282.3(PCCA): c.862A > G 5095 PCCA [′TGGGAATGCTTTATGGCTTAATGAANGAG (p.Arg288Gly) AGTGCTCAATTCAGAGAAGAAA′] 4291 NM_000288.3(PEX7): c.854A > G 5191 PEX7 [′TCTCTTCTTGAAACAGTGGAGCATCRTACA (p.His285Arg) GAGTTTACTTGTGGTTTAGAC′] 4292 NM_000532.4(PCCB): c.1606A > G 5096 PCCB [′ACAACGTCCTTGGAGAAAACATGCARATA (p.Asn536Asp) TTCCATTGTAAACAAATCAAAG′] 4293 NM_000553.4(WRN): c.2089 − 7486 WRN [′TCAAAGGAAAAATACATTTAAGATTVTAA 3024A > G GTCTGGTTATAAGCTTAAAAAG′] 4294 NM_001039958.1(MESP2): c.271A > 145873 MESP2 [′GCGGCAGAGCGCCAGCGAGCGGGAGRAA G (p.Lys91Glu) CTGCGCATGCGCACGCTGGCCCG′] 4295 NM_001099274.1(TINF2): c.850A > 26277 TINF2 [′TAGGGGAGGCCATAAGGAGCGCCCCRCAG G (p.Thr284Ala) TCATGCTGTTTCCCTTTAGGAA′] 4296 NM_001099274.1(TINF2): c.871A > 26277 TINF2 [′CCCCACAGTCATGCTGTTTCCCTTTRGGAA G (p.Arg291Gly) TCTCGGCTCACCAACCCAGGT′] 4297 NM_001363.4(DKC1): c.115A > G 1736 DKC1 [′ACAACACGCTGAAGAATTTCTTATCRAACC (p.Lys39Glu) TGAATCCAAAGTTGCTAAGTT′] 4298 NM_001363.4(DKC1): c.127A > G 1736 DKC1 [′AGAATTTCTTATCAAACCTGAATCCRAAGT (p.Lys43Glu) TGCTAAGTTGGACACGTCTCA′] 4299 NM_001363.4(DKC1): c.196A > G 1736 DKC1 [′GAATTTTGATAAGCTGAATGTAAGGRCAA (p.Thr66Ala) CACACTATACACCTCTTGCATG′] 4300 NM_001363.4(DKC1): c.941A > G 1736 DKC1 [′GTAAATGCCATCTGCTATGGGGCCARGATT (p.Lys314Arg) ATGCTTCCAGGTGTTCTTCGA′] 4301 NM_004614.4(TK2): c.173A > G 7084 TK2 [′GCATGTCGTCTTCCCACTTGCAATAYTGCC (p.Asn58Ser) CTCGACACAGATCTGGCAAAA′] 4302 NM_004614.4(TK2): c.278A > G 7084 TK2 [′AACCAACAACCCACTCACCAGAGGAYTGT (p.Asn93Ser) GGCCACGGACATTTCTCCACTT′] 4303 NM_004614.4(TK2): c.562A > G 7084 TK2 [′AGTTTACCTTCGGACCAATCCTGAGRCTTG (p.Thr188Ala) TTACCAGAGGTTAAAGAAGAG′] 4304 NM_024312.4(GNPTAB): c.1285 − 79158 GNPTAB [′TTTATTTTCTCTTTGGTTTTATCGTRGGTTT 2A > G ATTTGACATGGCCTGTGCCA′] 4305 NM_024312.4(GNPTAB): c.2867A > 79158 GNPTAB [′TTCACATCGCGGAAAGTCCCTGCTCRCATG G (p.His956Arg) CCTCACATGATTGACCGGATT′] 4306 NM_024312.4(GNPTAB): c.3458A > 79158 GNPTAB [′AGGAAGTTTGTTTGCCTGAATGACARCATT G (p.Asn1153Ser) GACCACAATCATAAAGATGCT′] 4307 NM_024312.4(GNPTAB): c.2783A > 79158 GNPTAB [′AGCAAAAATACTGGGAGGCAACTAARAGA G (p.Lys928Arg) TACATTTGCAGATTCCCTCAGA′] 4308 NM_198253.2(TERT): c.2537A > G 7015 TERT [′TCCACGCTGCTCTGCAGCCTGTGCTRCGGC (p.Tyr846Cys) GACATGGAGAACAAGCTGTTT′] 4309 NM_198578.3(LRRK2): c.3342A > G 120892 LRRK2 [′TAGAGAAACTGGAGCAGCTCATTTTRGAA (p.Leu1114=) GGGTAAGAAAGAGCTCATTAAA′] 4310 NM_198578.3(LRRK2): c.5605A > G 120892 LRRK2 [′TTTGGCTGACCTGCCTAGAAATATTRTGTT (p.Met1869Val) GAATAATGATGAGTTGGAATT′] 4311 NM_207352.3(CYP4V2): c.367A > G 285440 CYP4V2 [′TTCAAAGCAAATTGACAAATCCTCTRTGTA (p.Met123Val) CAAGTTTTTAGAACCATGGCT′] 4312 NM_207352.3(CYP4V2): c.761A > G 285440 CYP4V2 [′CTTATGTTTAAAGAAGGATGGGAACRCAA (p.His254Arg) AAAGAGCCTTCAGATCCTACAT′] 4313 NR_001566.1(TERC): n.37A > G 7012 TERC [′GGTGGGCCTGGGAGGGGTGGTGGCCDTTT TTTGTCTAACCCTAACTGAGAA′] 4314 NR_001566.1(TERC): n.48A > G 7012 TERC [′GAGGGGTGGTGGCCATTTTTTGTCTRACCC TAACTGAGAAGGGCGTAGGCG′] 4315 NM_007171.3(POMT1): c.430A > G 10585 POMT1 [′CATGTGTTTCCTCTTTGAAACAGAGRATGC (p.Asn144Asp) TCTCATCACTCAGTCAAGGCT′] 4316 NM_003863.3(DPM2): c.68A > G 8818 DPM2 [′GCCGTTAGCCTGATCATCTTCACCTRCTAC (p.Tyr23Cys) ACCGCCTGGGTGATTCTCTTG′] 4317 NM_000030.2(AGXT): c.1020A > G 189 AGXT [′GGAGAGACATCGTCAGCTACGTCATRGAC (p.Ile340Met) CACTTCGACATTGAGATCATGG′] 4318 NM_031418.2(ANO3): c.2053A > G 63982 ANO3 [′TTATGTTCTATTTCAGTGTCATCCTRGTGG (p.Ser685Gly) CTGTTTGATAGACCTCTGCCT′] 4319 NM_183075.2(CYP2U1): c.1139A > 113612 CYP2U1 [′CCTTTTTACATAGAAAAGGTTCATGRAGAA G (p.Glu380Gly) ATTGAAAGAGTCATTGGCGCC′] 4320 NM_003094.3(SNRPE): c.1A > G 6635 SNRPE [′GGTGTGCTCTTTGTGAAATTCCACCRTGGC (p.Met1Val) GTACCGTGGCCAGGGTCAGAA′] 4321 NM_000344.3(SMN1): c.389A > G 6606 SMN1 [′ACCTGTGTTGTGGTTTACACTGGATRTGGA (p.Tyr130Cys) AATAGAGAGGAGCAAAATCTG] 4322 NM_012082.3(ZFPM2): c.2209A > G −1 — [′CATGCAGAGAACCATGCGCACACGCRAGC (p.Lys737Glu) GCAGAAAGATGTATGAGATGTG′] 4323 NM_006364.2(SEC23A): c.2104A > 10484 SEC23A [′TGTTCAGTGTCAATGTATCTTGGCAYTGGA G (p.Met702Val) AATCTGGAGTGAAGAATTTCC′] 4324 NM_001139.2(ALOX12B): c.1562A > 242 ALOX12B [′ACCCTCCACGGCTGCGTCACTCGGGYAAT G (p.Tyr521Cys) AATAGGTGATGATCTCCGTCAC′] 4325 NM_001004434.2(SLC30A2): c.161A > 7780 SLC30A2 [′GTGACTGTCAGGACCCTTCTGAGCAYGGC > G (p.His54Arg) AGTGATGGTTGCTCTGGGCAGC′] 4326 NM_001168272.1(ITPR1): c.1759A 3708 ITPR1 [′CACTATCACTGCCCTGCTCCACAATRATCG > G (p.Asn587Asp) GAAACTCCTGGAAAAACACAT′] 4327 m.15923A > G −1 — [′ATAAACTAATACACCAGTCTTGTAARCCG GAGATGAAAACCTTTTTCCAAG′] 4328 NM_007332.2(TRPA1): c.2564A > G −1 — [′TTTTTTTACTTTTCTAGATTTGAAARTTGTG (p.Asn855Ser) GAATTTTTATTGTTATGTTG′] 4329 NM_014254.2(TMEM5): c.1016A > 10329 TMEM5 [′CCGGTCGGAGTAAACACAGAATGCTRTCG G (p.Tyr339Cys) AATCTATGAGGCTTGCTCCTAT′] 4330 NM_144577.3(CCDC114): c.487 − 93233 CCDC114 [′ACTGATTTTTTTCTCCTCCCCCCACRGGAG 2A > G ATCCACCACCTGCATCACCTG′] 4331 NM_000834.3(GRIN2B): c.2172 − 2904 GRIN2B [′GGATTTGTTTGCTTTTTTCCTGTACRGGAA 2A > G ACTGGATGCCTTCATCTATGA′] 4332 NM_018486.2(HDAC8): c.539A > G 55869 HDAC8 [′ATTCTCTACGTGGATTTGGATCTGCRCCAT (p.His180Arg) GGAGATGGTAAGCCCTTTGGT′] 4333 NM_018486.2(HDAC8): c.1001A > 55869 HDAC8 [′ACACTATCCTCTGAGATCCCAGATCRTGAG G (p.His334Arg) GTAAGTAAGGCTCTGACAAGT′] 4334 NM_178012.4(TUBB2B): c.767A > 347733 TUBB2B [′GCAGACCTGCGCAAGCTGGCGGTGARCAT G (p.Asn256Ser) GGTGCCCTTCCCTCGCCTGCAC′] 4335 NM_006888.4(CALM1): c.293A > G 801 CALM1 [′ATTGCTGAATGTTCACAGGATGGCARTGGT (p.Asn98Ser) TATATCAGTGCAGCAGAACTA′] 4336 NM_172107.2(KCNQ2): c.1636A > 3785 KCNQ2 [′GCCCCACCCACCCCCCTGCAGTGTCRTGCG G (p.Met546Val) GTTCCTGGTGTCCAAGCGGAA′] 4337 NM_001866.2(COX7B): c.41 − 2A > G 1349 COX7B [′TGTATTCTTTTTTCGTTTTCCTGTARGTTCG AAGCATTCAGCAAACAATGG′] 4338 NM_001099922.2(ALGl3): c.339A > 79868 ALGl3 [′TGAACAATCATCAGCTGGAACTGGCRAAG G (p.Ala113=) CAGCTACACAAAGAGGGTCATC′] 4339 NM_001083614.1(EARS2): c.502A > 124454 EARS2 [′CCCCATCAGGTATGACAATCGGTGCRGGA G (p.Arg168Gly) ACATGAGCCAGGAGCAGGTGGC′] 4340 NM_001083614.1(EARS2): c.193A > 124454 EARS2 [′TGCCTTGTACAACTACATCTTTGCTRAGAA G (p.Lys65Glu) GTACCAGGGGAGCTTCATCCT′] 4341 NM_033109.4(PNPT1): c.1160A > G 87178 PNPT1 [′ACCCTTCATGGATCAGCATTATTTCRAAGA (p.Gln387Arg) GGACAAACACAGGTAATTTAT′] 4342 NM_033109.4(PNPT1): c.1424A > G 87178 PNPT1 [′CCTTTCACCATAAGAGTTACATCTGRAGTC (p.Glu475Gly) CTAGAGTCAAATGGTATGGTA′] 4343 NM_000390.2(CHM): c.1520A > G 1121 CHM [′ATTTTTTTTTTAACAGATTTGGTTCRTTTGA (p.His507Arg) CTTGCACATCTTCTAAAACA′] 4344 NM_181690.2(AKT3): c.686A > G 10000 AKT3 [′TTGTGTTTTGTGATGGAATATGTTARTGGG (p.Asn229Ser) GGCGAGGTGAGTCAAGAAGTA′] 4345 NM_006567.3(FARS2): c.431A > G 10667 FARS2 [′CCCAGCAGGAAGAAGGGGGACAACTRTTA (p.Tyr144Cys) CCTGAATCGGACTCACATGCTG′] 4346 NM_001410.2(MEGF8): c.7099A > 1954 MEGF8 [′CAAGTGCCGGGAATCATTTCACGGGRGTC G (p.Ser2367 Gly) CGCTGGGCGGCCAGCAGTGCTA′] 4347 NM_003124.4(SPR): c.596 − 2A > G 6697 SPR [′CCTCATCGTCTCCTTTTCATCCTCTRGGTCC TCTGGACACAGACATGCAGC′] 4348 NM_005609.2(PYGM): c.152A > G 5837 PYGM [′GACCGCAATGTGGCCACCCCACGAGRCTA (p.Asp51Gly) CTACTTTGCTCTGGCCCATACC′] 4349 NM_004247.3(EFTUD2): c.623 A > 9343 EFTUD2 [′TTACTTAGTATTTGCTTTTCAGGACRTGTG G (p.His208Arg) AATTTCTCTGATGAGGTCACA′] 4350 NM_001310338.1(MGME1): c.743 92667 MGME1 [′GCTGTTCAACATGAAACCTTAAACTRTATA A > G (p.Tyr248Cys) GGTCTGCTGGACTGTGTGGCT′] 4351 NM_001128085.1(ASPA): c.433 − −1 — [′AAGAAAGACGTTTTTGATTTTTTTCRGACT 2A > G TCTCTGGCTCCACTACCCTGC′] 4352 NM_000108.4(DLD): c.1444A > G 1738 DLD [′TGGAGCATCCTGTGAAGATATAGCTRGAG (p.Arg482Gly) TCTGTCATGCACATCCGGTAAT′] 4353 NM_007215.3(POLG2): c.1105A > G 11232 POLG2 [′ACTAACATTAAGAACAAACAAACCCNTAT (p.Arg369Gly) TTTTAGTTTCCCAAGTCTATCT′] 4354 NM_004333.4(BRAF): c.2126A > G 673 BRAF [′AGAGATGAGAGACCACTCTTTCCCCRAGT (p.Gln709Arg) AAGTAAAAGCTTCATGCTATCC′] 4355 NM_004985.4(KRAS): c.65A > G 3845 KRAS [′GTAGGCAAGAGTGCCTTGACGATACDGCT (p.Gln22Arg) AATTCAGAATCATTTTGTGGAC′] 4356 NM_004985.4(KRAS): c.458A > G 3845 KRAS [′ACTTTTTATGTATTTCAGGGTGTTGDTGAT (p.Asp153Gly) GCCTTCTATACATTAGTTCGA′] 4357 NM_002834.3(PTPN11): c.124A > G 5781 PTPN11 [′TAGTAAAAGTAACCCTGGAGACTTCRCAC (p.Thr42Ala) TTTCCGTTAGGTAAGTTGGAAT′] 4358 NM_002834.3(PTPN11): c.172A > G 5781 PTPN11 [′AGCTGTCACCCACATCAAGATTCAGNACA (p.Asn58Asp) CTGGTGATTACTATGACCTGTA′] 4359 NM_002834.3(PTPN11): c.767A > G 5781 PTPN11 [′TTTCTTTCTTTCCAGACACTACAACRACAG (p.Gln256Arg) GAGTGCAAACTTCTCTACAGC′] 4360 NM_002834.3(PTPN11): c.844A > G 5781 PTPN11 [′CAAAAACAAAAATAGATATAAAAACRTCC (p.Ile282Val) TGCCCTGTAAGTATCAATATTC′] 4361 NM_002834.3(PTPN11): c.1510A > 5781 PTPN11 [′GATGGTGCGGTCTCAGAGGTCAGGGRTGG G (p.Met504Val) TCCAGACAGAAGCACAGTACCG′] 4362 NM_002880.3(RAF1): c.524A > G 5894 RAF1 [′ACTTGTGGCTACAAATTTCATGAGCRCTGT (p.His175Arg) AGCACCAAAGTACCTACTATG′] 4363 NA4_002880.3(RAF1): c.1279A > G 5894 RAF1 [′AATTGTGACCCAGTGGTGCGAGGGCRGCA (p.Ser427Gly) GCCTCTACAAACACCTGCATGT′] 4364 NM_005633.3(SOS1): c.508A >   6654 SOS1 [′TATTAAAGTGGCAATGTGTGCTGACRAGG (p.Lys170Glu) TAGGAAACTGAGCTTTTCTATT′] 4365 NM_030662.3(MAP2K2): c.181A > 5605 MAP2K2 [′GCGGCTGGAAGCCTTTCTCACCCAGRAAG G (p.Lys61Glu) CCAAGGTCGGCGAACTCAAAGA′] 4366 NM_024334.2(TMEM43): c.271A > 79188 TMEM43 [′GGAGAATGAAGGAAGGCTGGTGCACRTCA G (p.Ile91Val) TTGGCGCCTTACGGACATCCAA′] 4367 NM_000084.4(CLCN5): c.1637A > G 1184 CLCN5 [′CTGATGGCTGCAGCCATGACAAGCARGTG (p.Lys546Arg) GGTGGCAGATGCTCTTGGGCGG′] 4368 NM_000084.4(CLCN5): c.815A > G 1184 CLCN5 [′CCTTCTTTCTTCTAGGTCAGCTACTRTTTTC (p.Tyr272Cys) CCCTCAAAACATTGTGGCGT′] 4369 NM_000095.2(COMP): c.1760A > G 1311 COMP [′GGCGTGGACTTCGAGGGCACGTTCCRTGT (p.His587Arg) GAACACGGTCACGGATGACGAC′] 4370 NM_000530.6(MPZ): c.389A > G 4359 MPZ [′AATGGCACGTTCACTTGTGACGTCARAAA (p.Lys130Arg) CCCTCCAGACATAGTGGGCAAG′] 4371 NM_000530.6(MPZ): c.347A > G 4359 MPZ [′AAGGATGGCTCCATTGTCATACACARCCTA (p.Asn116Ser) GACTACAGTGACAATGGCACG′] 4372 NA4_003611.2(OFD1): c.260A > G 8481 OFD1 [′GCAGATCACTTACAAAGATGTGGCTDTGA (p.Tyr87Cys) ATATTCACTTTCTGTTTTCTTT′] 4373 NA4_003611.2(OFD1): c.290A > G 8481 OFD1 [′TATTCACTTTCTGTTTTCTTTCCAGRAAGTG (p.Glu97Gly) GTTTGGCAAAAGAAAAGGTA′] 4374 NA4_003611.2(OFD1): c.382 − 2A > G 8481 OFD1 [′TAAAAGTGAAATATTTTCTTTTAACRGGTT TCAGGATCTGATAAAGAAAAT′] 4375 NM_003977.3(AIP): c.721A > G 9049 AIP [′GCTGCTGCTCAACTACTGCCAGTGCDAGCT (p.Lys241Glu) GGTGGTCGAGGAGTACTACGA′] 4376 NM_006158.4(NEFL): c.293A > G 4747 NEFL [′GAGAAGGCGCAGCTCCAGGACCTCARTGA (p.Asn98Ser) CCGCTTCGCCAGCTTCATCGAG′] 4377 NA4_024312.4(GNPTAB): c.3053A > 79158 GNPTAB [′ATATCTCAAGTCTTTGATGAAGTTGRTACA G (p.Asp1018Gly) GATCAATCTGGTGTCTTGTCT′] 4378 NA4_025137.3(SPGl1): c.1457 − 80208 SPG11 [′TAAAGCTAACTTTTATTTTTCCTATRGAGA 2A > G ATGGACTCTCTCTGATTTTGT′] 4379 NM_025137.3(SPGl1): c.2608A > G 80208 SPG11 [′ACAAGAATCCATCCTTCTCCCCAGGRTAAG (p.Ile870Val) TCCAGAAGGCAAGTGTGAGAG′] 4380 NM_025137.3(SPGl1): c.2833A > G 80208 SPG11 [′GAATGAAATTTTAGATAAGCTGGCCRGGT (p.Arg945Gly) ATTATAACTGTTGAACTAATAC′] 4381 NA4_025137.3(SPGl1): c.6477 + 4A > 80208 SPG11 [′CAGTGAGGAGTATGGGCTGGTGGTARGTA G GCCCCCTCAACCCCAGTCTCCA′] 4382 NM_212472.2(PRKAR1A): c.178 − 5573 PRKAR1A [′ATTTTGCAAACTCGTAATTTCTTTCRGGAG 2A > G GAGGCAAAACAGATTCAGAAT′] 4383 NM_006231.3(POLE): c.4444 + 3A > 5426 POLE [′CCAGTTCAGCTACCTGGAACCAGGTRTGG G CCTGCACCAGCCGCCCATCATG′] 4384 NM_003156.3(STEV11): c.251A > G 6786 STEV11 [′GACGATGATGCCAATGGTGATGTGGRTGT (p.Asp84Gly) GGAAGAAAGTGATGAGGTGAGC′] 4385 NM_003156.3(STEV11): c.326A > G 6786 STEV11 [′CCAACAGTGAAACACAGCACCTTCCRTGG (p.His109Arg) TGAGGATAAGCTCATCAGCGTG′] 4386 NM_001128425.1(MUTYH): c.934 − 4595 MUTYH [′GGCTAAGAGCTGTTCCTGCTCCACCYGAG 2A > G AGGCACAGGGTTGAGTGTCATA′] 4387 NM_133499.2(SYN1): c.1699A > G 6853 SYN1 [′GGCGGAGCCGGGCCAGAGACGGATGYCTG (p.Thr567Ala) ACGGGTAGCCTGTGGGGGGCCC′] 4388 NM_021629.3(GNB4): c.265A > G 59345 GNB4 [′TATTTGGGATAGCTATACAACAAATRAGG (p.Lys89Glu) TAGAATTTCTTCATAATTCTTT′] 4389 NM_138425.3(C12orf57): c.1A > G 113246 C12orf57 [′CTGAACCTAGAGCTTCAGACGCCCTRTGGC (p.Met1Val) GTCCGCCTCGACCCAACCGGC′] 4390 NM_000096.3(CP): c.2953A > G 1356 CP [′GGGAGATGAAGTCAACTGGTATCTGRTGG (p.Met985Val) GAATGGGCAATGAAATAGACTT′] 4391 NM_000057.3(BLM): c.1088 − 2A > G 641 BLM [′AATATTAACAACATAATTATTTTATRGCTA GACAGATAAGTTTACAGCAGC′] 4392 NM_000257.3(MYH7): c.5807A > G 4625 MYH7 [′TTTCAAAAGGGCTTGAATGAGGAGTRGCT (p.Ter1936Trp) TTGCCACATCTTGATCTGCTCA′] 4393 NM_152263.3(TPM3): c.505A > G 7170 TPM3 [′GGATCTTTTCCTGTAGGTGGCTCGTRAGTT (p.Lys169Glu) GGTGATCATTGAAGGAGACTT′] 4394 NM_152263.3(TPM3): c.733A > G 7170 TPM3 [′AGAGACCCGTGCTGAGTTTGCTGAGRGAT (p.Arg245Gly) CGGTAGCCAAGCTGGAAAAGAC′] 4395 NM_000096.3(CP): c.1209 − 2A > G 1356 CP [′GCATTAAACACTTTTTTCCCCCTGCRGTGA CTCAGCGGTGTTTTTTGAACA′] 4396 NM_002739.3(PRKCG): c.76A > G 5582 PRKCG [′CCTGTTTTGCAGAAAGGGGGCCCTGRGGC (p.Arg26Gly) AGAAGGTGGTCCACGAAGTCAA′] 4397 NM_000138.4(FBN1): c.1148 − 2200 FBN1 [′GTGTTTTGTTTTGTTGTGTTTTTCTRGAGGA 2A > G TTTCAACAAGCTGTGCTCTG′] 4398 NM_000138.4(FBN1): c.3058A > G 2200 FBN1 [′ACCCGGATTTGCCACAAAAGAAATTRCAA (p.Thr1020Ala) ATGGAAAGCCTTTCTTCAAAGG′] 4399 NM_000169.2(GLA): c.1153A > G −1 — [′GGCCTGTAATCCTGCCTGCTTCATCRCACA (p.Thr385Ala) GCTCCTCCCTGTGAAAAGGAA′] 4400 NM_000256.3(MYBPC3): c.1224 − 4607 MYBPC3 [′ACTTCAACGGCCCCTTCTGTTCTACRGCAA 2A > G GTAAGTTCCCCTCTGGATGGC′] 4401 NM_000256.3(MYBPC3): c.1814A > 4607 MYBPC3 [′GTCAGCCTCGTCGGCAGGTGTGACGYCGT G (p.Asp605Gly) CAATGGTCAGTTTGTGGACCCT′] 4402 NM_000256.3(MYBPC3): c.1928 − 4607 MYBPC3 [′CTCTGAACTACATTGTGTCTTCTGCRGAAC 2A > G CTCCCAAGATCCACCTGGACT′] 4403 NM_000256.3(MYBPC3): c.2309 − 4607 MYBPC3 [′GGGCCGCAGGTGCGTCTGGCACGTCBGGA 2A > G TGGGGTGGGATGGACCCACATC′] 4404 NM_000256.3(MYBPC3): c.26 − 4607 MYBPC3 [′GTGGCTTCTTGCTAAAAGCTGAGACYGAA 2A > G GGGCCAGGTGGAGGCTACAGCG′] 4405 NM_000256.3(MYBPC3): c.927 − 4607 MYBPC3 [′GGCCACAGCCTAGACTGCGGGACACRGGG 2A > G ACTCGAAGCTGGAGGCACCAGC′] 4406 NM_000257.3(MYH7): c.2206A > G 4625 MYH7 [′AGCGGCCATCCCTGAGGGACAGTTCRTTG (p.Ile736Val) ATAGCAGGAAGGGGGCAGAGAA′] 4407 NM_000257.3(MYH7): c.2681A > G 4625 MYH7 [′CTCCCCTCTGTTCCTCACCTTCAGGDACAA (p.Glu894Gly) GACAACCTGGCAGATGCTGAG′] 4408 NM_000260.3(MYO7A): c.1344 − 4647 MYO7A [′CCTTACCCCATCCCTGTGCCCCTGCRGCTT 2A > G TGAGCAGCTCTGCATCAACTT′] 4409 NM_000260.3(MYO7A): c.6029A > 4647 MYO7A [′GTGCCAGGGAAGGATCCCATGGCCGRTTC G (p.Asp2010Gly) CATCTTCCACTATTACCAGGTG′] 4410 NM_000260.3(MYO7A): c.6439 − 4647 MYO7A [′CTCTCTATGCCCTTTCTGCTCCCCCRGGAT 2A > G ATCCTCACCACTCATCCCTTC′] 4411 NM_000441.1(SLC26A4): c.-3 − −1 — [′CCCTCCTCGCTGTCCTCTGGCTCGCRGGTC 2A > G ATGGCAGCGCCAGGCGGCAGG′] 4412 NM_000441.1(SLC26A4): c.1149 + 3A > 5172 SLC26A4 [′ATTACACCATCGATGGGAACCAGGTDTGG G GTGCCCTTTTGCTGAACTGGTT′] 4413 NM_000551.3(VHL): c.467A > G 7428 VHL [′GATTTGGTTTTTGCCCTTCCAGTGTRTACTC (p.Tyr156Cys) TGAAAGAGCGATGCCTCCAG] 4414 NM_002294.2(LAMP2): c.65 − 2A > G 3920 LAMP2 [′AAATATTATTTTTTTAAATGAATCCRGGAG CTGTGCGGTCTTATGCATTGG′] 4415 NM_004004.5(GJB2): c.1A > G 2706 GJB2 [′CAGAGCAAACCGCCCAGAGTAGAAGRTGG (p.Met1Val) ATTGGGGCACGCTGCAGACGAT′] 4416 NM_004004.5(GJB2): c.617A > G 2706 GJB2 [′GTGTCTGGAATTTGCATCCTGCTGARTGTC (p.Asn206Ser) ACTGAATTGTGTTATTTGCTA′] 4417 NM_033360.3(KRAS): c.182A > G 3845 KRAS [′TTGGATATTCTCGACACAGCAGGTCNAGA (p.Gln6lArg) GGAGTACAGTGCAATGAGGGAC′] 4418 NM_006218.2(PIK3CA): c.1637A > 5290 PIK3CA [′GATCCTCTCTCTGAAATCACTGAGCRGGAG G (p.Gln546Arg) AAAGATTTTCTATGGAGTCAC′] 4419 NM_006218.2(PIK3CA): c.3073A > 5290 PIK3CA [′TGATGACATTGCATACATTCGAAAGRCCCT G (p.Thr1025Ala) AGCCTTAGATAAAACTGAGCA′] 4420 NM_032119.3(ADGRV1): c.14973 − 84059 ADGRV1 [′TTCTTCATGATTTAATTTTTTTCCCRGATCA 2A > G GGTTTCATTGTTGCTGAAAT′] 4421 NM_033056.3(PCDH15): c.1998 − 65217 PCDH15 [′TTATTTGTTTGTTTGTTTTGTCACTRGCACG 2A > G GGGATTCTAACCTTAGGGAA′] 4422 NM_138691.2(TMC1): c.1763 + 3A > 117531 TMC1 [′ATCTTCAACCAAGGCATGATCTGGTRGGCC G AGCTGTTGGACAGCTTATCAC′] 4423 NM_206933.2(USH2A): c.12067 − 7399 USH2A [′CAACTTAACCTGTTAATTTTCTTACRGGGA 2A > G ACAAGCCATCAAGCCCACCTG′] 4424 NM_206933.2(USH2A): c.12295 − 7399 USH2A [′CCCGTCACTGAAGATGTTGTATGTCYACAG 2A > G AAGGACAGAAGCAAAAGGGAT′] 4425 NM_206933.2(USH2A): c.1841 − 7399 USH2A [′ATAATGCATAACCTTTCCCTGATGCRGGAA 2A > G GGAACTGTGAGCTGTGCAAGG′] 4426 NM_206933.2(USH2A): c.8559 − 7399 USH2A [′GATGTTCCTGCTTGTCTTTTGCTTTRGATAT 2A > G GAGCTTCTGAGACGTAAAAT′] 4427 NM_03241S.5(CARD11): c.401A > 84433 CARD11 [′GGCCTCACGCACTTCCTGATGAACGRGGTC G (p.Glu134Gly) ATCAAGCTGCAGCAGCAGATG′] 4428 NM_000548.3(TSC2): c.226 − 2A > G 7249 TSC2 [′CACCGCTGTCCCCTCTGCTGGTGACRGCAC GCAGTGGAAGCACTCTGGAAG′] 4429 NM_000548.3(TSC2): c.1444 − 2A > G 7249 TSC2 [′TCATTGGCCTCCCTTGTGCCTGTGCRGGAG GAGCTGATTAACTCAGTGGTC′] 4430 NM_000548.3(TSC2): c.2546 − 2A > G 7249 TSC2 [′CCCTGACCACCCTCTCCATTACCGCDGCTC TGGCCAGGCTGCCGCACCTCT′] 4431 NM_130466.3(UBE3B): c.545 − 89910 UBE3B [′TTTGTTCTCACTGTTTTCTTTCTTTRGGTGA 2A > G AAGTCTTCGACCAGCGATGA′] 4432 NM_005430.3(WNT1): c.624 + 4A > 7471 WNT1 [′CAACAACGAGGCAGGCCGTACGGTGRGCT G TTGAGAGGCTCCGCACCCTAAG′] 4433 NM_001893.4(CSNK1D): c.137A > 1453 CSNK1D [′AAGCTTGAATGTGTCAAAACCAAACRCCC G (p.His46Arg) TCAGCTCCACATTGAGAGCAAA′] 4434 NM_018972.2(GDAP1): c.368A > G 54332 GDAP1 [′AGCATGTATTACCCACGGGTACAACRTTAC (p.His123Arg) CGAGAGCTGCTTGACTCCTTG′] 4435 NM_032237.4(POMK): c.773A > G 84197 POMK [′CATGGGGATTTCGTGGCTCCAGAGCRACT (p.Gln258Arg) GTGGCCCTATGGAGAGGACGTG′] 4436 NM_201647.2(STAMBP): c.125A > 10617 STAMBP [′CGTCGGTACTTCCGCTCTGGAGTTGRGATT G (p.Glu42Gly) ATCCGAATGGCATCCATTTAC′] 4437 NM_001946.3(DUSP6): c.566A > G 1848 DUSP6 [′ACTACCATCCGAGTCTGTTGCACTAYTGGG (p.Asn189Ser) GTCTCGGTCAAGGTCAGACTC′] 4438 NM_003867.3(FGF17): c.560A > G 8822 FGF17 [′TACCAAGGCCAGCTGCCCTTCCCCARCCAC (p.Asn187Ser) GCCGAGAAGCAGAAGCAGTTC′] 4439 NM_013281.3(FLRT3): c.1016A > G −1 — [′GGGCTCATGTGCCAAGCCCCAGAAARGGT (p.Lys339Arg) TCGTGGGATGGCTATTAAGGAT′] 4440 NM_015560.2(OPA1): c.1146A > G 4976 OPA1 [′TTTTTATTTTTCCTGAGTAGACCATRTCCTT (p.Ile382Met) AAATGTAAAAGGCCCTGGAC′] 4441 NM_030964.3(SPRY4): c.530A > G 81848 SPRY4 [′CCGGCGGTCCCACCCGAGCTGGACARGCA (p.Lys177Arg) CTTCTTGCTGTGCGAGGCCTGT′] 4442 NM_002972.3(SBF1): c.1249A > G 6305 SBF1 [′AAGGCCATGCCCTCCAGCACCTTCAYCAG (p.Met417Val) GAAATCGTCCTCTACCAGCCCA′] 4443 NM_002972.3(SBF1): c.4768A > G 6305 SBF1 [′TACATGTAATTGTGGAACACAGGCGYCCT (p.Thr1590Ala) CTTGCTCAGCCGGTCCACATAC′] 4444 NM_006876.2(B4GAT1): c.1168A > 11041 B4GAT1 [′GTTCCATCCCCAAAAGGAGGCTGAARATC G (p.Asn390Asp) AGCACAATAAGATCCTATATCG′] 4445 NM_000059.3(BRCA2): c.517 − 675 BRCA2 [′AAAATAAACTATTTTCTTTCCTCCCVGGGT 2A > G CGTCAGACACCAAAACATATT′] 4446 NM_000059.3(BRCA2): c.6938 − 675 BRCA2 [′ATGTAATATAAAATAATTGTTTCCTRGGCA 2A > G CAATAAAAGATCGAAGATTGT′] 4447 NM_000059.3(BRCA2): c.7008 − 675 BRCA2 [′AACTTATATATTTTCTCCCCATTGCDGCAC 2A > G AACTAAGGAACGTCAAGAGAT′] 4448 NM_000059.3(BRCA2): c.7806 − 675 BRCA2 [′GATAATATTCTACTTTTATTTGTTCRGGGC 2A > G TCTGTGTGACACTCCAGGTGT′] 4449 NM_000059.3(BRCA2): c.8754 + 4A > 675 BRCA2 [′AGCAGACCCAGCTTACCTTGAGGTGRGAG G AGTAAGAGGACATATAATGAGG′] 4450 NM_000059.3(BRCA2): c.9118 − 675 BRCA2 [′GTTGAATTTTTGTTTTGTTTTCTGTRGGTTT 2A > G CAGATGAAATTTTATTTCAG′] 4451 NM_000059.3(BRCA2): c.9649 − 675 BRCA2 [′TAGGCTACGTTTTCATTTTTTTATCRGATGT 2A > G CTTCTCCTAATTGTGAGATA′] NM_000218.2(KCNQ1): c.1070A > 3784 KCNQ1 [] G (p.Gln357Arg) 4452 NM_000218.2(KCNQ1): c.1085A > 3784 KCNQ1 [′AAGGTGCAGCAGAAGCAGAGGCAGARGC G (p.Lys362Arg) ACTTCAACCGGCAGATCCCGGCG] 4453 NM_000218.2(KCNQ1): c.1576A > 3784 KCNQ1 [′TCGACGCATGCAGTACTTTGTGGCCVAGA G (p.Lys526Glu) AGAAATTCCAGGTAAGCCCTGT′] NM_000218.2(KCNQ1): c.1756A > 3784 KCNQ1 [] G (p.Asn586Asp) 4454 NM_000218.2(KCNQ1): c.332A > G 3784 KCNQ1 [′CGCACCCACGTCCAGGGCCGCGTCTRCAA (p.Tyr111Cys) CTTCCTCGAGCGTCCCACCGGC′] NM_000218.2(KCNQ1): c.344A > G 3784 KCNQ1 [] (p.Glu115Gly) 4455 NM_000218.2(KCNQ1): c.773A > G 3784 KCNQ1 [′CTCCTGGGCTCCGTGGTCTTCATCCVCCGC (p.His258Arg) CAGGTGGGTGGCCCGGGTTAG′] 4456 NM_000218.2(KCNQ1): c.944A > G 3784 KCNQ1 [′CAGGTCACAGTCACCACCATCGGCTNTGG (p.Tyr315Cys) GGACAAGGTGCCCCAGACGTGG′] NM_000218.2(KCNQ1): c.964A > G 3784 KCNQ1 [] (p.Thr322Ala) 4457 NM_000492.3(CFTR): c.1393 − 1080 CFTR [′CCTAATAATGATGGGTTTTATTTCCRGACT 2A > G TCACTTCTAATGGTGATTATG′] 4458 NM_000492.3(CFTR): c.1766 + 3A > 1080 CFTR [′ACAGAAAAAGAAATATTTGAAAGGTVTGT G TCTTTGAATACCTTACTTATAA′] 4459 NM_000492.3(CFTR): c.1A > G 1080 CFTR [′CAGGGACCCCAGCGCCCGAGAGACCRTGC (p.Met1Val) AGAGGTCGCCTCTGGAAAAGGC′] 4460 NM_000492.3(CFTR): c.2989 − 1080 CFTR [′ACCAACATGTTTTCTTTGATCTTACDGTTG 2A > G TTATTAATTGTGATTGGAGCT′] 4461 NM_000492.3(CFTR): c.579 + 3A > G 1080 CFTR [′ACAACCTGAACAAATTTGATGAAGTNTGT ACCTATTGATTTAATCTTTTAG′] 4462 NM_007294.3(BRCA1): c.122A > G 672 BRCA1 [′GAACCTGTCTCCACAAAGTGTGACCRCAT (p.His41Arg) ATTTTGCAAGTAAGTTTGAATG′] 4463 NM_007294.3(BRCA1): c.1A > G 672 BRCA1 [′TTAAAGTTCATTGGAACAGAAAGAARTGG (p.Met1Val) ATTTATCTGCTCTTCGCGTTGA′] 4464 NM_007294.3(BRCA1): c.212 + 3A > 672 BRCA1 [′TGTAAGAATGATATAACCAAAAGGTRTAT G AATTTGGTAATGATGCTAGGTT′] 4465 NM_007294.3(BRCA1): c.4097 − 672 BRCA1 [′TCTGAACCTCTGTTTTTGTTATTTAVGGTG 2A > G AAGCAGCATCTGGGTGTGAGA′] 4466 NM_007294.3(BRCA1): c.4485 − 672 BRCA1 [′GTTTTCTCCTTCCATTTATCTTTCTRGGTCA 2A > G TCCCCTTCTAAATGCCCATC′] 4467 NM_007294.3(BRCA1): c.4987 − 672 BRCA1 [′ATAATGGAATATTTGATTTAATTTCRGATG 2A > G CTCGTGTACAAGTTTGCCAGA′] 4468 NM_007294.3(BRCA1): c.5053A > 672 BRCA1 [′AACTAATCTAATTACTGAAGAGACTRCTCA G (p.Thr1685Ala) TGTTGTTATGAAAACAGGTAT′] 4469 NM_007294.3(BRCA1): c.5407 − 672 BRCA1 [′AATGCTCTTTCCTTCCTGGGGATCCDGGGT 2A > G GTCCACCCAATTGTGGTTGTG′] 4470 NM_000540.2(RYR1): c.97A > G 6261 RYR1 [′CGCTACCGTGCTCAAGGAGCAGCTCRAGC (p.Lys33Glu) TCTGCCTGGCCGCCGAGGGCTT′] 4471 NM_015250.3(BICD2): c.2321A > G 23299 BICD2 [′CGGCAGCTGGCGGCTGCTGAGGACGRGAA (p.Glu774Gly) GAAGACGCTGAACTCGCTGCTG′] 4472 NM_006012.2(CLPP): c.270 + 4A > G 8192 CLPP [′CATCGTGTGCGTCATGGGCCCGGTGRGCG CCCCGCGCCGGGACCCTCCCCA′] 4473 NM_004656.3(BAP1): c.277A > G 8314 BAP1 [′AGGAGCACGCTCAGCAAGGCATGAGHTGC (p.Thr93Ala) ACAAGAGTTGGGTATCAGCTGT′] 4474 NM_004315.4(ASAH1): c.965 + 4A > 427 ASAH1 [′AGGAATCATTGGATGTATATGAGTARGTA G GGTTTGTTAAAGCAAAAGAAGT′] 4475 NM_000100.3(CSTB): c.169 − 2A > G 1476 CSTB [′GCTTCGCTCACTCCGCTCTCTTCCCRGGTG CACGTCGGCGACGAGGACTTC′] 4476 NM_015268.3(DNAJC13): c.2564A > 23317 DNAJC13 [′TTAAAATCTAGGTATGAATTTTTCARTGAG G (p.Asn855Ser) CTTTATCATCGCTTCTTGCTC′] 4477 NM_001003722.1(GLE1): c.433 − 2733 GLE1 [′CTTAAAAAAAAAAAAAAAAAAAAAARCCT 10A > G TTTCAGGAGGGCCTGAGGCTAT′] 4478 NM_001142519.1(FAM111A): c.1583A > 63901 FAM111A [′TTCCAGAAAATAGTTCACAACCCTGDTGTG G (p.Asp528Gly) ATTACCTATGACACTGAATTT′] 4479 NM_001142519.1(FAM111A): c.1012A > 63901 FAM111A [′GCATAGAACAACGTTTGGGAAAGTARCAA G (p.Thr338Ala) AAAATTCTTCTTCGATTAAAGT′] 4480 NM_001927.3(DES): c.1289 − 2A > G 1674 DES [′CTGAGTGTGCGATGGACCCTGTTACRGAA ACCAGCCCTGAGCAAAGGGGTT′] 4481 NM_014795.3(ZEB2): c.3134A > G 9839 ZEB2 [′AAACACAAGCACCACCTTATCGAGCRCTC (p.His1045Arg) AAGGCTTCACTCGGGCGAGAAG′] 4482 NM_002163.2(IRF8): c.322A > G 3394 IRF8 [′CCAACTGGACATTTCCGAGCCATACRAAG (p.Lys108Glu) TTTACCGAATTGTTCCTGAGGA′] 4483 NM_002163.2(IRF8): c.238A > G 3394 IRF8 [′CAAAGCTGAACCAGCCACTTGGAAGRCGA (p.Thr80Ala) GGTTACGCTGTGCTTTGAATAA′] 4484 NM_001287.5(CLCN7): c.296A > G 1186 CLCN7 [′TGTCCCGGCCTGCAGAGCTTGGACTRTGAC (p.Tyr99Cys) AACAGTGAGAACCAGCTGTTC′] 4485 NM_001127217.2(SMAD9): c.127A > 4093 SMAD9 [′AGAGAAGGCAGTGGACTCTCTAGTGRAGA G (p.Lys43Glu) AGTTAAAGAAGAAGAAGGGAGC′] 4486 NM_002246.2(KCNK3): c.575A > G 3777 KCNK3 [′TGGACCTTCTTCCAGGCCTACTACTRCTGC (p.Tyr192Cys) TTCATCACCCTCACCACCATC′] 4487 NM_080605.3(B3GALT6): c.1A > G 126792 B3GALT6 [′CGCCACGCCCGCCGCAGCAGCTTCAYGGC (p.Met1Val) GCCCGCGCCGGGCCGGCGGCCC′] 4488 NM_080605.3(B3GALT6): c.193A > 126792 B3GALT6 [′CGGCGCTCGGCGGCGCGGGGCGCGCYGGC G (p.Ser65Gly) CACCAGCACTGCCAGGAAGGCG′] 4489 NM_000388.3(CASR): c.85A > G 846 CASR [′CGGGCCAGACCAGCGAGCCCAAAAGRAGG (p.Lys29Glu) GGGACATTATCCTTGGGGGGCT′] 4490 NM_003793.3(CTSF): c.962A > G 8722 CTSF [′GGGACCCTGCTCTCCCTCTCTGAACRGGGT (p.Gln321Arg) GAGCATCTCGCTCTACTCCTC′] 4491 NM_003793.3(CTSF): c.692A > G 8722 CTSF [′GAGATCACTGAACTTGGTGACTCCAYACT (p.Tyr231Cys) GAGCTGTGCCACGGTCCAGGGC′] 4492 NM_022114.3(PRDM16): c.2447A > 63976 PRDM16 [′GGCAGCCGGGCCCGTGCCAGCCAAARCGG G (p.Asn816Ser) CGGCGGGCGGGAGCCCCGCAAG′] 4493 NM_173551.4(ANKS6): c.1322A > 203286 ANKS6 [′GGGCAGCACTGGGATGCTCCAGGGCYGTC G (p.Gln441Arg) GGACCTTCGAGTGGGGCAGGGG′] 4494 NM_005689.2(ABCB6): c.508A > G 10058 ABCB6 [′GAACTTGGCCCTGGTGTCTTGGAACRGCCC (p.Ser170Gly) ACAGTGGTGGTGGGCAAGGGC′] 4495 NM_001070.4(TUBG1): c.275A > G 7283 TUBG1 [′AAGCTCTACAACCCAGAGAACATCTRCCT (p.Tyr92Cys) GTCGGAACATGGAGGAGGAGCT′] 4496 NM_001613.2(ACTA2): c.145A > G 59 ACTA2 [′ACCTTTTTAGGGGGTGATGGTGGGARTGG (p.Met49Val) GACAAAAAGACAGCTACGTGGG′] 4497 NM_031157.2(HNRNPA1): c.956A > 3178 HNRNPA1 [′AGCTACAATGATTTTGGGAATTACARCAAT G (p.Asn319Ser) CAGTCTTCAAATTTTGGACCC′] 4498 NM_020117.9(LARS): c.1118A > G 51520 LARS [′CTTAATAGTTAGCATTGGGAGAACAYAGA (p.Tyr373Cys) TCACCTTGTATGATGTTAAAGG′] 4499 NM_001651.3(AQP5): c.367A > G 362 AQP5 [′CTAACCCGCTATCCCCTTGCAGCTCRACAA (p.Asn123Asp) CAACACAACGCAGGGCCAGGC′] 4500 m.13637A > G 4540 MT-NDS [′ATAATTCTTCTCACCCTAACAGGTCRACCT CGCTTCCCCACCCTTACTAAC′] 4501 NM_000081.3(LYST): c.10127A > G 1130 LYST [′GGGAAGGCTTCTGTTCAAGCGATCARTGTT (p.Asn3376Ser) TTTCATCCTGCTGTAAGTGAC′] 4502 NM_000142.4(FGFR3): c.2421A > G 2261 FGFR3 [′CCAGCAGTGGGGGCTCGCGGACGTGNAGG (p.Ter807Trp) GCCACTGGTCCCCAACAATGTG′] 4503 NM_000207.2(INS): c.*59A > G −1 — [′TCCTGCACCGAGAGAGATGGAATAARGCC CTTGAACCAGCCCTGCTGTGCC′] 4504 NM_000370.3(TTPA): c.191A > G 7274 TTPA [′CTGCGCGCCCGGGATTTCGATCTGGRCCTG (p.Asp64Gly) GCCTGGCGGGTAAGCGTGCGT′] 4505 NM_001006657.1(WDR35): c.2912A > 57539 WDR35 [′AAACCTTTACGTGTCAAGAAGCTCTRTGTA G (p.Tyr971Cys) CTGTCAGCCTTACTTATAGAG′] 4506 NM_004595.4(SMS): c.443A > G 6611 SMS [′GTATATGACGAAGATTCACCTTATCRAAAT (p.Gln148Arg) ATAAAAATTCTACACTCGAAG′] 4507 NM_005259.2(MSTN): c.458A > G −1 — [′AAAATACAATACAATAAAGTAGTAARGGC (p.Lys153Arg) CCAACTATGGATATATTTGAGA′] 4508 NM_000784.3(CYP27A1): c.1061A > 1593 CYP27A1 [′TGGGCCCTGTACCACCTCTCAAAGGRCCCT G (p.Asp354Gly) GAGATCCAGGAGGCCTTGCAC′] 4509 NM_000784.3(CYP27A1): c.776A > 1593 CYP27A1 [′TCACTCTATGCCACCTTCCTCCCCARGTGG G (p.Lys259Arg) ACTCGCCCCGTGCTGCCTTTC′] 4510 NM_000540.2(RYR1): c.10100A > G 6261 RYR1 [′ATCCCAACTATCGGGCGGCTGCGCARGAG (p.Lys3367Arg) GGCAGGGAAGGTGGTGTCCGAG′] 4511 NM_000540.2(RYR1): c.14572A > G 6261 RYR1 [′CTACCTGTACACCGTGGTGGCCTTCRACTT (p.Asn4858Asp) CTTCCGCAAGTTCTACAACAA′] 4512 NM_000540.2(RYR1): c.14740A > G 6261 RYR1 [′CGCGGGTGACGAATACGAGCTCTACRGGG (p.Arg4914Gly) TGGTCTTCGACATCACCTTCTT′] 4513 NM_000540.2(RYR1): c.14591A > G 6261 RYR1 [′GCCTTCAACTTCTTCCGCAAGTTCTRCAAC (p.Tyr4864Cys) AAGAGCGAGGATGAGGATGAA′] 4514 NM_002336.2(LRP6): c.1298A > G 4040 LRP6 [′GATCGAATAGAAGTGACAAGGCTCARTGG (p.Asn433Ser) GACCATGAGGAAGATCTTGATT′] 4515 NM_001257235.1(ALGl3): c.8A > G 79868 ALG13 [′GTTATAAACGAAAAGTTGATGAACARTCA (p.Asn3Ser) TCAGCTGGAACTGGCAAAGCAG′] 4516 NM_001015879.1(AURKC): c.379 − 6795 AURKC [′CAGGGTGACTTTTCTTTGCACCCACRGATA 2A > G ATAGAGGAGTTGGCAGATGCC′] 4517 NM_012160.4(FBXL4): c.1694A > G 26235 FBXL4 [′AATTGTACCAGGTTACAGCAGCTGGRCAT (p.Asp565Gly) ATTAGGTAAGGTTACAATATAT′] 4518 NM_020988.2(GNAO1): c.521A > G 2775 GNAO1 [′GCCGACTACCAGCCCACCGAGCAGGRCAT (p.Asp174Gly) CCTCCGAACCAGGGTCAAAACC′] 4519 NM_001927.3(DES): c.1024A > G 1674 DES [′GACCTGGGTTCCCCCTCTCCTGCAGRACGA (p.Asn342Asp) TTCCCTGATGAGGCAGATGCG′] 4520 NM_001927.3(DES): c.1333A > G 1674 DES [′GGGTTCTGAGGTCCATACCAAGAAGRCGG (p.Thr445Ala) TGATGATCAAGACCATCGAGAC′] 4521 NM_001927.3(DES): c.735 + 3A > G 1674 DES [′TCCTTAAGAAAGTGCATGAAGAGGTRTAC CTTGGCCCCTCTTCCTGGGGTC′] 4522 NM_002055.4(GFAP): c.1097A > G 2670 GFAP [′GCCCTGGACATCGAGATCGCCACCTRCAG (p.Tyr366Cys) GAAGCTGCTAGAGGGCGAGGAG′] 4523 NM_002055.4(GFAP): c.1112A > G 2670 GFAP [′ATCGCCACCTACAGGAAGCTGCTAGDGGG (p.Glu371Gly) CGAGGAGAACCGGTGAGCCCTC′] 4524 NM_002055.4(GFAP): c.1121A > G 2670 GFAP [′TACAGGAAGCTGCTAGAGGGCGAGGRGAA (p.Glu374Gly) CCGGTGAGCCCTCATCACAGCC′] 4525 NM_002055.4(GFAP): c.230A > G 2670 GFAP [′GAGCGGGCAGAGATGATGGAGCTCARTGA (p.Asn77Ser) CCGCTTTGCCAGCTACATCGAG′] 4526 NM_002055.4(GFAP): c.770A > G 2670 GFAP [′AACATGCATGAAGCCGAAGAGTGGTRCCG (p.Tyr257Cys) CTCCAAGGTAGCCCTGCCTGTG′] 4527 NM_002055.4(GFAP): c.835A > G 2670 GFAP [′CAACGCGGAGCTGCTCCGCCAGGCCRAGC (p.Lys279Glu) ACGAAGCCAACGACTACCGGCG′] 4528 NM_005554.3(KRT6A): c.511A > G 3853 KRT6A [′GGAGCGTGAACAGATCAAGACCCTCDACA (p.Asn171Asp) ACAAGTTTGCCTCCTTCATCGA′] 4529 NM_005554.3(KRT6A): c.512A > G 3853 KRT6A [′GAGCGTGAACAGATCAAGACCCTCAVCAA (p.Asn171Ser) CAAGTTTGCCTCCTTCATCGAC′] 4530 NM_005557.3(KRT16): c.373A > G 3868 KRT16 [′TGAGAAGGTGACCATGCAGAACCTCRATG (p.Asn125Asp) ACCGCCTGGCCTCCTACCTGGA′] 4531 NM_170707.3(LMNA): c.640 − 4000 LMNA [′TTTTGGTTTCTGTGTCCTTCCTCCAVCCCTT 10A > G CCAGGAGCTGCGTGAGACCA′] NM_000218.2(KCNQ1): c.1061A > 3784 KCNQ1 [] G (p.Lys354Arg) NM_000218.2(KCNQ1): c.1138A > 3784 KCNQ1 [] G (p.Arg380Gly) NM_000218.2(KCNQ1): c.1193A > 3784 KCNQ1 [] G (p.Lys398Arg) NM_000218.2(KCNQ1): c.1640A > 3784 KCNQ1 [] G (p.Gln547Arg) NM_000218.2(KCNQ1): c.1669A > 3784 KCNQ1 [] G (p.Lys557Glu) NM_000218.2(KCNQ1): c.1705A > 3784 KCNQ1 [] G (p.Lys569Glu) NM_000218.2(KCNQ1): c.1793A > 3784 KCNQ1 [] G (p.Lys598Arg) NM_000218.2(KCNQ1): c.430A > G 3784 KCNQ1 [] (p.Thr144Ala) NM_000218.2(KCNQ1): c.440A > G 3784 KCNQ1 [] (p.Gln147Arg) NM_000218.2(KCNQ1): c.548A > G 3784 KCNQ1 [] (p.Lys183Arg) 4532 NM_000218.2(KCNQ1): c.592A > G 3784 KCNQ1 [′GCGGCTGCGCTTTGCCCGGAAGCCCRTTTC (p.Ile198Val) CATCATCGGTGAGTCATGCCT′] 4533 NM_000218.2(KCNQ1): c.652A > G 3784 KCNQ1 [′CATGGTGGTCCTCTGCGTGGGCTCCRAGGG (p.Lys218Glu) GCAGGTGTTTGCCACGTCGGC′] 4534 NM_000218.2(KCNQ1): c.820A > G 3784 KCNQ1 [′CCTGTACATCGGCTTCCTGGGCCTCRTCTT (p.Ile274Val) CTCCTCGTACTTTGTGTACCT′] NM_000218.2(KCNQ1): c.842A > G 3784 KCNQ1 [] (p.Tyr281Cys) NM_000218.2(KCNQ1): c.931A > G 3784 KCNQ1 [] (p.Thr311Ala) NM_000218.2(KCNQ1): c.950A > G 3784 KCNQ1 [] (p.Asp317Gly) NM_000238.3(KCNH2): c.1205A > 3757 KCNH2 [] G (p.His402Arg) NM_000238.3(KCNH2): c.1259A > 3757 KCNH2 [] G (p.Tyr420Cys) 4535 NM_000238.3(KCNH2): c.1280A > 3757 KCNH2 [′ATCTACACGGCTGTCTTCACACCCTVCTCG G (p.Tyr427Cys) GCTGCCTTCCTGCTGAAGGAG′] NM_000238.3(KCNH2): c.128A > G 3757 KCNH2 [] (p.Tyr43Cys) NM_000238.3(KCNH2): c.1424A > 3757 KCNH2 [] G (p.Tyr475Cys) 4536 NM_000238.3(KCNH2): c.1478A > 3757 KCNH2 [′CACCCCGGCCGCATCGCCGTCCACTNCTTC G (p.Tyr493Cys) AAGGGCTGGTTCCTCATCGAC′] NM_000238.3(KCNH2): c.1502A > 3757 KCNH2 [] G (p.Asp501Gly) 4537 NM_000238.3(KCNH2): c.1685A > 3757 KCNH2 [′ATGTGCACCTTTGCGCTCATCGCGCVCTGG G (p.His562Arg) CTAGCCTGCATCTGGTACGCC′] 4538 NM_000238.3(KCNH2): c.1711A > 3757 KCNH2 [′CTGGCTAGCCTGCATCTGGTACGCCVTCGG G (p.Ile571Val) CAACATGGAGCAGCCACACAT′] 4539 NM_000238.3(KCNH2): c.1720A > 3757 KCNH2 [′CTGCATCTGGTACGCCATCGGCAACVTGG G (p.Met574Val) AGCAGCCACACATGGACTCACG′] NM_000238.3(KCNH2): c.1724A > 3757 KCNH2 [] G (p.Glu575Gly) 4540 NM_000238.3(KCNH2): c.173A > G 3757 KCNH2 [′GAGCTGTGCGGCTACTCGCGGGCCGVGGT (p.Glu58Gly) GATGCAGCGACCCTGCACCTGC′] NM_000238.3(KCNH2): c.1747A > 3757 KCNH2 [] G (p.Ile583Val) NM_000238.3(KCNH2): c.1762A > 3757 KCNH2 [] G (p.Asn588Asp) NM_000238.3(KCNH2): c.1777A > 3757 KCNH2 [] G (p.Ile593Val) NM_000238.3(KCNH2): c.1783A > 3757 KCNH2 [] G (p.Lys595Glu) NM_000238.3(KCNH2): c.1790A > 3757 KCNH2 [] G (p.Tyr597Cys) NM_000238.3(KCNH2): c.1826A > 3757 KCNH2 [] G (p.Asp609Gly) NM_000238.3(KCNH2): c.1847A > 3757 KCNH2 [] G (p.Tyr616Cys) NM_000238.3(KCNH2): c.1885A > 3757 KCNH2 [] G (p.Asn629Asp) 4541 NM_000238.3(KCNH2): c.1886A > 3757 KCNH2 [′AGCCTCACCAGTGTGGGCTTCGGCANCGT G (p.Asn629Ser) CTCTCCCAACACCAACTCAGAG′] NM_000238.3(KCNH2): c.1897A > 3757 KCNH2 [] G (p.Asn633Asp) 4542 NM_000238.3(KCNH2): c.1898A > 3757 KCNH2 [′GTGGGCTTCGGCAACGTCTCTCCCADCACC G (p.Asn633Ser) AACTCAGAGAAGATCTTCTCC′] NM_000238.3(KCNH2): c.1903A > 3757 KCNH2 [] G (p.Asn635Asp) NM_000238.3(KCNH2): c.1910A > 3757 KCNH2 [] G (p.Glu637Gly) NM_000238.3(KCNH2): c.1912A > 3757 KCNH2 [] G (p.Lys638Glu) 4543 NM_000238.3(KCNH2): c.1933A > 3757 KCNH2 [′AGAGAAGATCTTCTCCATCTGCGTCDTGCT G (p.Met645Val) CATTGGCTGTGAGTGTGCCCA′] NM_000238.3(KCNH2): c.209A > G 3757 KCNH2 [] (p.His70Arg) 4544 NM_000238.3(KCNH2): c.2131A > 3757 KCNH2 [′GCACGCCTGGTCCTACACCAACGGCRTCG G (p.Ile711Val) ACATGAACGCGGTGAGGCCACC′] NM_000238.3(KCNH2): c.2266A > 3757 KCNH2 [] G (p.Met756Val) NM_000238.3(KCNH2): c.2510A > 3757 KCNH2 [] G (p.Asp837Gly) NM_000238.3(KCNH2): c.2591A > 3757 KCNH2 [] G (p.Asp864Gly) NM_000238.3(KCNH2): c.286A > G 3757 KCNH2 [] (p.Ile96Val) 4545 NM_000238.3(KCNH2): c.301A > G 3757 KCNH2 [′CAAAGTGGAAATCGCCTTCTACCGGDAAG (p.Lys101Glu) ATGGTAGGAGCGGGCCGGGGCG′] 4546 NM_000238.3(KCNH2): c.3118A > 3757 KCNH2 [′TCGGCGGCCCCGGGGCGACGTGGAGRGCA G (p.Ser1040Gly) GGCTGGATGCCCTCCAGCGCCA′] NM_000238.3(KCNH2): c.3233A > 3757 KCNH2 [] G (p.Tyr1078Cys) NM_000238.3(KCNH2): c.3343A > 3757 KCNH2 [] G (p.Met1115Val) NM_000238.3(KCNH2): c.652A > G 3757 KCNH2 [] (p.Met218Val) NM_000238.3(KCNH2): c.82A > G 3757 KCNH2 [] (p.Lys28Glu) NM_000891.2(KCNJ2): c.220A > G 3759 KCNJ2 [] (p.Thr74Ala) NM_000891.2(KCNJ2): c.223A > G 3759 KCNJ2 [] (p.Thr75Ala) NM_000891.2(KCNJ2): c.233A > G 3759 KCNJ2 [] (p.Asp78Gly) NM_000891.2(KCNJ2): c.574A > G 3759 KCNJ2 [] (p.Thr192Ala) 4547 NM_000891.2(KCNJ2): c.913A > G 3759 KCNJ2 [′CATACTGGAAGGCATGGTGGAAGCCVCTG (p.Thr305Ala) CCATGACGACACAGTGCCGTAG′] NM_172201.1(KCNE2): c.269A > G 9992 KCNE2 [] (p.Glu90Gly) 4548 NM_172201.1(KCNE2): c.281A > G 9992 KCNE2 [′CAGTACATTGTAGAGGACTGGCAGGRAAA (p.Glu94Gly) GTACAAGAGCCAAATCTTGAAT′] NM_000335.4(SCN5A): c.1217A > G 6331 SCN5A [] (p.Asn406Ser) NM_000335.4(SCN5A): c.1502A > G 6331 SCN5A [] (p.Asp501Gly) NM_000335.4(SCN5A): c.2249A > G 6331 SCN5A [] (p.Gln750Arg) NM_198056.2(SCN5A): c.2527A > G 6331 SCN5A [] (p.Thr843Ala) NM_000335.4(SCN5A): c.2780A > G 6331 SCN5A [] (p.Asn927Ser) NM_000335.4(SCN5A): c.3164A > G 6331 SCN5A [] (p.Asp1055Gly) NM_000335.4(SCN5A): c.343A > G 6331 SCN5A [] (p.Ser115Gly) NM_000335.4(SCN5A): c.3755A > G 6331 SCN5A [] (p.Glu1252Gly) 4549 NM_000335.4(SCN5A): c.376A > G 6331 SCN5A [′AAAGGATATGAGTGAACCAGAATCTYCAC (p.Lys126Glu) AGCCGCTCTCCGGATGGGGTGG′] 4550 NM_198056.2(SCN5A): c.4000A > G 6331 SCN5A [′TGCCCTGGTGGGCGCCATCCCGTCCRTCAT (p.Ile1334Val) GAACGTCCTCCTCGTCTGCCT′] NM_000335.4(SCN5A): c.4223A > G 6331 SCN5A [] (p.Tyr1408Cys) NM_000335.4(SCN5A): c.4252A > G 6331 SCN5A [] (p.Lys1418Glu) NM_000335.4(SCN5A): c.4291A > G 6331 SCN5A [] (p.Arg1431Gly) NM_198056.2(SCN5A): c.4346A > G 6331 SCN5A [] (p.Tyr1449Cys) NM_000335.4(SCN5A): c.4412A > G 6331 SCN5A [] (p.Asn1471Ser) 4551 NM_198056.2(SCN5A): c.4478A > G 6331 SCN5A [′ATCTTCATGACAGAGGAGCAGAAGARGTA (p.Lys1493Arg) CTACAATGCCATGAAGAAGCTG′] NM_000335.4(SCN5A): c.4489A > G 6331 SCN5A [] (p.Met1497Val) NM_000335.4(SCN5A): c.4577A > G 6331 SCN5A [] (p.Lys1526Arg) 4552 NM_198056.2(SCN5A): c.4978A > G 6331 SCN5A [′GATGTCCCTGCCTGCCCTCTTCAACRTCGG (p.Ile1660Val) GCTGCTGCTCTTCCTCGTCAT′] NM_000335.4(SCN5A): c.5138A > G 6331 SCN5A [] (p.Asp1713Gly) NM_000335.4(SCN5A): c.5161A > G 6331 SCN5A [] (p.Asn1721Asp) NM_000335.4(SCN5A): c.5297A > G 6331 SCN5A [] (p.Tyr1766Cys) NM_198056.2(SCN5A): c.5302A > G 6331 SCN5A [] (p.Ile1768Val) NM_000335.4(SCN5A): c.5317A > G 6331 SCN5A [] (p.Asn1773Asp) NM_000335.4(SCN5A): c.5318A > G 6331 SCN5A [] (p.Asn1773Ser) NM_000335.4(SCN5A): c.5366A > G 6331 SCN5A [] (p.Asp1789Gly) NM_000335.4(SCN5A): c.5402A > G 6331 SCN5A [] (p.Asp1801Gly) NM_000335.4(SCN5A): c.5513A > G 6331 SCN5A [] (p.Asp1838Gly) NM_198056.2(SCN5A): c.5726A > G 6331 SCN5A [] (p.Gln1909Arg) 4553 NM_000335.4(SCN5A): c.688A > G 6331 SCN5A [′CCGAGTCCTCCGGGCCCTGAAAACTRTATC (p.Ile230Val) AGTCATTTCAGGTGAAAATCA′] NM_000335.4(SCN5A): c.715A > G 6331 SCN5A [] (p.Ile239Val) NM_000335.4(SCN5A): c.89A > G 6331 SCN5A [] (p.Glu30Gly) 4554 NM_000383.3(AIRE): c.254A > G 326 AIRE [′TTCTGGAGGGTGCTGTTCAAGGACTRCAAC (p.Tyr85Cys) CTGGAGCGCTATGGCCGGCTG′] 4555 NM_006920.4(SCN1A): c.1876A > G 6323 SCN1A [′ACGCAACAGCAACCTGAGTCAGACCRGTA (p.Ser626Gly) GGTCATCCCGGATGCTGGCAGT′] 4556 NM_006920.4(SCN1A): c.4352A > G −1 — [′GAAGAAAGTCTGTACATGTATCTTTRCTTT (p.Tyr1451Cys) GTTATTTTCATCATCTTTGGG′] 4557 NM_000165.4(GJA1): c.617A > G 2697 GJA1 [′TGTTTCCTCTCTCGCCCCACGGAGARAACC (p.Lys206Arg) ATCTTCATCATCTTCATGCTG′] 4558 NM_000833.4(GRIN2A): c.1123 − 2903 GRIN2A [′AGCTGTGTCTTTGTGTTTGTGCTGCRGGTG 2A > G GGCAAGTGGGAGAACCATACG′] 4559 NC_000007.14: g.62535490A > G −1 — [′GGAGAGTTTTGAGGCCTGTGGTTGARATG GAAATATCTTCACAGAAAAACT′] 4560 NM_004595.4(SMS): c.983A > G 6611 SMS [′AATCTGACAGAAGCACTGTCGCTCTVTGA (p.Tyr328Cys) AGAACAGCTGGGGCGCCTGTAT′] 4561 NM_001256864.1(DNAJC6): c.801 − 9829 DNAJC6 [′CAAGAGAGTGCCAACCTTCTGTTTCRGATA 2A > G CCTGGGCTATATGTGTGACCT′] 4562 NM_001204316.1(PRLR): c.635A > 5618 PRLR [′GTCCAGGTTCGCTGCAAACCAGACCRTGG G (p.His212Arg) ATACTGGAGTGCATGGAGTCCA′] 4563 NM_012243.2(SLC35A3): c.886A > 23443 SLC35A3 [′TTGGCTTCAAGATTTTGTGCCAACCRGGTA G (p.Ser296Gly) AAATGTTCTTTTCTATTTTTT′] 4564 NM_000179.2(MSH6): c.3439 − 2956 MSH6 [′AAAGACCTTTTCCTCCCTCATTCACRGGCT 2A > G GGCTTATTAGCTGTAATGGCC′] 4565 NM_000249.3(MLH1): c.113A > G 4292 MLH1 [′AATGCTATCAAAGAGATGATTGAGARCTG (p.Asn38Ser) GTACGGAGGGAGTCGAGCCGGG′] 4566 NM_000249.3(MLH1): c.122A > G 4292 MLH1 [′TTTTCTGTTTGATTTGCCAGTTTAGRTGCA (p.Asp41Gly) AAATCCACAAGTATTCAAGTG′] 4567 NM_000249.3(MLH1): c.1559 − 4292 MLH1 [′TTTTTGGTTTTATTTTTTGTTTTGCNGTTCT 2A > G CCGGGAGATGTTGCATAACC′] 4568 NM_000249.3(MLH1): c.1990 − 4292 MLH1 [′TTGAGGTATTGAATTTCTTTGGACCRGGTG 2A > G AATTGGGACGAAGAAAAGGAA′] 4569 NM_000249.3(MLH1): c.208 − 2A > G 4292 MLH1 [′TTTACTCATCTTTTTGGTATCTAACVGAAA GAAGATCTGGATATTGTATGT′] 4570 NM_000249.3(MLH1): c.544A > G 4292 MLH1 [′TGGGAAAATTTTGGAAGTTGTTGGCRGGT (p.Arg182Gly) ACAGTCCAAAATCTGGGAGTGG′] 4571 NM_000249.3(MLH1): c.545 + 3A > 4292 MLH1 [′AAAATTTTGGAAGTTGTTGGCAGGTRCAGT G CCAAAATCTGGGAGTGGGTCT′] 4572 NM_000249.3(MLH1): c.546 − 2A > G 4292 MLH1 [′TCTTACTCTTTTGTTTTTCTTTTCCVGGTAT TCAGTACACAATGCAGGCAT′] 4573 NM_000249.3(MLH1): c.589 − 2A > G 4292 MLH1 [′TTGTGTCTTCTGCTGTTTGTTTATCRGCAAG GAGAGACAGTAGCTGATGTT′] 4574 NM_000249.3(MLH1): c.677 + 3A > 4292 MLH1 [′GATTTTTTTATATAGGTTATCGACABACCG G ACTAACAGCATTTCCAAAGAT′] 4575 NM_000249.3(MLH1): c.791 − 2A > G 4292 MLH1 [′ACTGGTTGCTTTCTTTTTATTGTTTRGATCG TCTGGTAGAATCAACTTCCT′] 4576 NM_000249.3(MLH1): c.883A > G 4292 MLH1 [′AAACACACACCCATTCCTGTACCTCVGGTA (p.Ser295Gly) ATGTAGCACCAAACTCCTCAA′] 4577 NM_000249.3(MLH1): c.884 + 4A > 4292 MLH1 [′CACACCCATTCCTGTACCTCAGGTARTGTA G GCACCAAACTCCTCAACCAAG′] 4578 NM_000251.2(MSH2): c.1277 − 4436 MSH2 [′TTTGTTTGTTTTACTACTTTCTTTTVGGAAA 2A > G ACACCAGAAATTATTGTTGG′] 4579 NM_000251.2(MSH2): c.1511 − 4436 MSH2 [′CTTTTTCTTTTCTTCTTGATTATCARGGCTT 2A > G GGACCCTGGCAAACAGATTA′] 4580 NM_000251.2(MSH2): c.1660A > G 4436 MSH2 [′GAAGAATGGTGTTAAATTTACCAACNGGT (p.Ser554Gly) TTGCAAGTCGTTATTATATTTT′] 4581 NM_000535.5(PMS2): c.1A > G 5395 PMS2 [′CCGAGGCGGATCGGGTGTTGCATCCDTGG (p.Met1Val) AGCGAGCTGAGAGCTCGAGGTG′] 4582 NM_000535.5(PMS2): c.989 − 2A > G 5395 PMS2 [′ATAAATATGTTTTCTTTTTTGCCTTRGAATG CGTTGATATCAATGTTACTC′] 4583 NM_000059.3(BRCA2): c.426 − 675 BRCA2 [′TAAGGGATTTGCTTTGTTTTATTTTRGTCCT 2A > G GTTGTTCTACAATGTACACA′] 4584 NM_024876.3(ADCK4): c.857A > G 79934 ADCK4 [′CAGCAGGAGCTGGCTTGGGAGTGTGRCTA (p.Asp286Gly) CCGTCGTGAGGCGGCTTGTGCC′] 4585 NM_006005.3(WFS1): c.1385A > G 7466 WFS1 [′TACACGCGCAGGGCCCTGGCCACCGRGGT (p.Glu462Gly) CACCGCCGGCCTGCTATCGCTG′] 4586 NM_000019.3(ACAT1): c.472A > G 38 ACAT1 [′GGCAGGTGGGATGGAGAGCATGTCCRATG (p.Asn158Asp) TTCCATATGTAATGAACAGAGG′] 4587 NM_000050.4(ASS1): c.496 − 2A > G 445 ASS1 [′TCCACCTGTGCTGTCTCTTTCCTGCRGCAA CACGGGATTCCCATCCCGGTC′] 4588 NM_000117.2(EMD): c.450 − 2A > G 2010 EMD [′CCCACTTGCTCCCCTCTTTTGCCTCRGGGA ACGCCCCATGTACGGCCGGGA′] 4589 NM_000153.3(GALC): c.334A > G 2581 GALC [′GCATAATGCATGTGGGAGGGCTCAGBGCC (p.Thr112Ala) GTCTGAATAGAGGAGAGCAAAA′] 4590 NM_000155.3(GALT): c.905 − 2A > G 2592 GALT [′CCCCACTGTCTCTCTTCTTTCTGTCRGGGG CTCCCACAGGATCAGAGGCTG′] 4591 NM_000159.3(GCDH): c.542A > G 2639 GCDH [′CTCCTGGGCTGCTTCGGGCTCACAGRGCCC (p.Glu181Gly) AACAGCGGAAGTGACCCCAGC′] 4592 NM_000169.2(GLA): c.509A > G −1 — [′TGGGGAGTAGATCTGCTAAAATTTGRTGGT (p.Asp170Gly) TGTTACTGTGACAGTTTGGAA′] 4593 NM_000169.2(GLA): c.548 − 2A > G −1 — [′TATTTTACCCATTGTTTTCTCATACRGGTTA TAAGCACATGTCCTTGGCCC′] 4594 NM_000169.2(GLA): c.647A > G −1 — [′TCATATCTGTTTTCACAGCCCAATTRTACA (p.Tyr216Cys) GAAATCCGACAGTACTGCAAT′] 4595 NM_000232.4(SGCB): c.1A > G 6443 SGCB [′GCTCGGCGGCGGCGGGCGCGGGAAGRTGG (p.Met1Val) CGGCAGCGGCGGCGGCGGCTGC′] 4596 NM_000255.3(MUT): c.1445 − 2A > G 4594 MUT [′TAAAATTTTTCTTTGGGAAATTACCRGGTT CTGAAGTAATTGTTGGAGTAA′] 4597 NM_000277.1(PAH): c.533A > G 5053 PAH [′AGTGGGCAGCCCATCCCTCGAGTGGDATA (p.Glu178Gly) CATGGAGGAAGAAAAGAAAACA′] 4598 NM_000277.1(PAH): c.974A > G 5053 PAH [′CAGATTGACTTTCCATTCCAGATTTRCTGG (p.Tyr325Cys) TTTACTGTGGAGTTTGGGCTC] 4599 NM_000328.2(RPGR): c.155 − 2A > G 6103 RPGR [′TGATTATTTCTTTTTCCCTCCTACCRGGAA ATAATAAACTTTACATGTTTG′] 4600 NM_000350.2(ABCA4): c.4540 − 24 ABCA4 [′ATTAACGTGGGTGTCTCATTGCCTCRGAGA 2A > G ACACAGCGCAGCACGGAAATT′] 4601 NM_000350.2(ABCA4): c.67 − 2A > G 24 ABCA4 [′TTCTCTCTTTTTGTTTTGTTTTTCCRGATTC GCTTTGTGGTGGAACTCGTG′] 4602 NM_000487.5(ARSA): c.1108 − 410 ARSA [′CCCCGTGACCCCTGACTCTGCCCCCRGAGC 2A > G CCTCGGCAGTCTCTCTTCTTC′] 4603 NM_000489.4(ATRX): c.134 − 2A > G 546 ATRX [′TAATGTTCTTTATTTCTTCTTTTTTRGATAA AATCAGTGGTTCTGGAAGTA′] 4604 NM_000489.4(ATRX): c.536A > G 546 ATRX [′TGCACTGCTTGTGGACAACAGGTCARTCAT (p.Asn179Ser) TTTCAAAAAGATTCCATTTAT′] 4605 NM_000512.4(GALNS): c.1171A > 2588 GALNS [′CTTCTATTACCGTGGCGACACGCTGRTGGC G (p.Met391Val) GGCCACCCTCGGGCAGCACAA′] 4606 NM_000521.3(HEXB): c.1243 − 3074 HEXB [′ATGTATTGCAATTTGTAACGTTAATRGCTT 2A > G GCGCCGGGCACAATAGTTGAA′] 4607 NM_000531.5(OTC): c.238A > G 5009 OTC [′ATAGTATTTGCCTTTATTGCAAGGGRAGTC (p.Lys80Glu) CTTAGGCATGATTTTTGAGAA′] 4608 NM_000551.3(VHL): c.233A > G 7428 VHL [′GAGCCCTCCCAGGTCATCTTCTGCARTCGC (p.Asn78Ser) AGTCCGCGCGTCGTGCTGCCC′] 4609 NM_001848.2(COL6A1): c.805 − 1291 COL6A1 [′CCAACCTTGACCTGTTTTGTGTTCCRGGGA 2A > G GAACGAGGCAAGCCGGGGCTC′] 4610 NM_001918.3(DBT): c.773 − 2A > G 1629 DBT [′AATGAATAACAATTTAATGCTTTTCRGGCT TTCAAAAAGCAATGGTCAAGA′] 4611 NM_003482.3(KMT2D): c.5645 − 8085 KMT2D [′TCAACAGTGTCCTTCATTCCCCCACRGAAC 2A > G TGCCCAAGATGGAATCCAAGG′] 4612 NM_003494.3(DYSF): c.1398 − 8291 DYSF [′CAGGCCCTCTCTGCTCCCTTGCTCTRGGGA 2A > G CCGCCTGACTCACAATGACAT′] 4613 NM_004006.2(DMD): c.1332 − 9A > G 1756 DMD [′AAGAGGTCATAATAGGCTTCTTTCAVATTT TCAGTTTACATAGAGTTTTAA′] 4614 NM_004006.2(DMD): c.3432 + 3A > 1756 DMD [′AGTGGGATCACATGTGCCAACAGGTRTAG G ACAATCTCTTTCACTGTGGCTT′] 4615 NM_004006.2(DMD): c.6763 − 2A > G 1756 DMD [′AAACGTTGTTGCATTTGTCTGTTTCRGTTA CTGGTGGAAGAGTTGCCCCTG′] 4616 NM_004006.2(DMD): c.9224 + 61934A > 1756 DMD [′AGCTGTGAATGCTTCATTCAGGCCCRAGTA G AATATAGGAAGAGGTGTAGTG′] 4617 NM_004006.2(DMD): c.9225 − 1756 DMD [′GCTGTGAATGCTTCATTCAGGCCCARGTAA 647A > G ATATAGGAAGAGGTGTAGTGG′] 4618 NM_004006.2(DMD): c.9650 − 2A > G 1756 DMD [′TGTTGCAATTTTCTTCTTCCTTTGTRGACCT TTTCAAGCAAGTGGCAAGTT′] 4619 NM_015560.2(OPA1): c.983A > G 4976 OPA1 [′GAGATGATGACACGTTCTCCAGTTAVGGT (p.Lys328Arg) AAGAACATAGGCCGTCTCAGTG′] 4620 NM_144997.5(FLCN): c.1433 − 201163 FLCN [′TTGCTCTGCCCCTGCCCTTCTCCCCRGTGG 2A > G GCCCCACCATCCTGAATAAGA′] 4621 NM_144997.5(FLCN): c.250 − 2A > G 201163 FLCN [′AGATTTACTTTTCCTTTTCATGGACRGGGC TGCCGGTCACTTGCTGCAGGG′] 4622 NM_000146.3(FTL): c.1A > G 2512 FTL [′GTTAGCTCCTTCTTGCCAACCAACCRTGAG (p.Met1Val) CTCCCAGATTCGTCAGAATTA′] 4623 NM_024531.4(SLC52A2): c.914A > 79581 SLC52A2 [′GTGCAGAGCTTTTCCTGCTTACCCTRCGGG G (p.Tyr305Cys) CGTCTGGCCTACCACCTGGCT′] 4624 NM_001743.5(CALM2): c.293A > G 805 CALM2 [′TACTAAATTTTTTGCTAGGATGGCADTGGC (p.Asn98Ser) TATATTAGTGCTGCAGAACTT′] 4625 NM_014754.2(PTDSS1): c.1058A > 9791 PTDSS1 [′ACACAGTGCAAGCGCGTAGGAACACRATG G (p.Gln353Arg) CTGGGTGTTTGGGTGAGTAATC′] 4626 NM_052844.3(WDR34): c.1307A > 89891 WDR34 [′GGACCAGCGCACAGCAAACAGATACYTGA G (p.Lys436Arg) GGGAGAGCTGCAGCGAAGTCAA′] 4627 NM_001130010.2(C15orf41): c.281A > 84529 C15orf41 [′TACTTCTGTTTTATAAAGGTGGACTVTGCG G (p.Tyr94Cys) CCCTCATTAATGGCTCGGCTT′] 4628 NM_001290048.1(ATL3): c.521A > 25923 ATL3 [′AATTTCATCCATTGCCAGACGACCGYATTC G (p.Tyr174Cys) TGTGAAGAGCTTTAAAAAAGA′] 4629 NM_000531.5(OTC): c.1034A > G 5009 OTC [′GTCATGGTGTCCCTGCTGACAGATTRCTCA (p.Tyr345Cys) CCTCAGCTCCAGAAGCCTAAA′] 4630 NM_000531.5(OTC): c.122A > G 5009 OTC [′AATAAAGTGCAGCTGAAGGGCCGTGRCCT (p.Asp41Gly) TCTCACTCTAAAAAACTTTACC′] 4631 NM_000531.5(OTC): c.155A > G 5009 OTC [′ACTCTAAAAAACTTTACCGGAGAAGRAAT (p.Glu52Gly) TAAATATATGCTATGGCTATCA′] 4632 NM_000531.5(OTC): c.1A > G 5009 OTC [′TCGTCCTTTACACAATTAAAAGAAGDTGCT (p.Met1Val) GTTTAATCTGAGGATCCTGTT′] 4633 NM_000531.5(OTC): c.268A > G 5009 OTC [′CTTAGGCATGATTTTTGAGAAAAGARGTA (p.Ser90Gly) CTCGAACAAGATTGTCTACAGA′] 4634 NM_000531.5(OTC): c.277A > G 5009 OTC [′GATTTTTGAGAAAAGAAGTACTCGARCAA (p.Thr93Ala) GATTGTCTACAGAAACAGGTAA′] 4635 NM_000531.5(OTC): c.350A > G 5009 OTC [′TGTTTTCTTACCACACAAGATATTCDTTTG (p.His117Arg) GGTGTGAATGAAAGTCTCACG′] 4636 NM_000531.5(OTC): c.377A > G 5009 OTC [′TTGGGTGTGAATGAAAGTCTCACGGRCAC (p.Asp126Gly) GGCCCGGTTTGTAAATATTTTC′] 4637 NM_000531.5(OTC): c.387 − 2A > G 5009 OTC [′GATTATCTTTTTCTTGGTTTGCCACNGTGT ATTGTCTAGCATGGCAGATGC′] 4638 NM_000531.5(OTC): c.481A > G 5009 OTC [′TAAAGAAGCATCCATCCCAATTATCRATG (p.Asn161Asp) GGCTGTCAGATTTGTACCATCC′] 4639 NM_000531.5(OTC): c.482A > G 5009 OTC [′AAAGAAGCATCCATCCCAATTATCARTGG (p.Asn161Ser) GCTGTCAGATTTGTACCATCCT′] 4640 NM_000531.5(OTC): c.503A > G 5009 OTC [′ATCAATGGGCTGTCAGATTTGTACCVTCCT (p.His168Arg) ATCCAGATCCTGGCTGATTAC′] 4641 NM_000531.5(OTC): c.524A > G 5009 OTC [′TACCATCCTATCCAGATCCTGGCTGDTTAC (p.Asp175Gly) CTCACGCTCCAGGTTGGTTTA′] 4642 NM_000531.5(OTC): c.527A > G 5009 OTC [′CATCCTATCCAGATCCTGGCTGATTRCCTC (p.Tyr176Cys) ACGCTCCAGGTTGGTTTATTT] 4643 NM_000531.5(OTC): c.541 − 2A > G 5009 OTC [′TCATCTCCTTCATCCCGTGCCTTTTRGGAA CACTATAGCTCTCTGAAAGGT′] 4644 NM_000531.5(OTC): c.542A > G 5009 OTC [′TCTCCTTCATCCCGTGCCTTTTAGGRACAC (p.Glu181Gly) TATAGCTCTCTGAAAGGTCTT′] 4645 NM_000531.5(OTC): c.548A > G 5009 OTC [′TCATCCCGTGCCTTTTAGGAACACTRTAGC (p.Tyr183Cys) TCTCTGAAAGGTCTTACCCTC] 4646 NM_000531.5(OTC): c.595A > G 5009 OTC [′CCTCAGCTGGATCGGGGATGGGAACRATA (p.Asn199Asp) TCCTGCACTCCATCATGATGAG′] 4647 NM_000531.5(OTC): c.596A > G 5009 OTC [′CTCAGCTGGATCGGGGATGGGAACARTAT (p.Asn199Ser) CCTGCACTCCATCATGATGAGC′] 4648 NM_000531.5(OTC): c.613A > G 5009 OTC [′TGGGAACAATATCCTGCACTCCATCRTGAT (p.Met205Val) GAGCGCAGCGAAATTCGGAAT′] 4649 NM_000531.5(OTC): c.716A > G 5009 OTC [′AAGTTGGCAGAGCAGTATGCCAAAGDGGT (p.Glu239Gly) ATGCTCTTTACATGTAAAGCTA′] 4650 NM_000531.5(OTC): c.717 + 3A > G 5009 OTC [′TGGCAGAGCAGTATGCCAAAGAGGTRTGC TCTTTACATGTAAAGCTATTAT′] 4651 NM_000531.5(OTC): c.718 − 2A > G 5009 OTC [′TTTAACCAGCGTGTTTATGTATGCTRGAAT GGTACCAAGCTGTTGCTGACA′] 4652 NM_000531.5(OTC): c.788A > G 5009 OTC [′CATGGAGGCAATGTATTAATTACAGRCAC (p.Asp263 Gly) TTGGATAAGCATGGGACAAGAA′] 4653 NM_000531.5(OTC): c.790A > G 5009 OTC [′TGGAGGCAATGTATTAATTACAGACRCTTG (p.Thr264Ala) GATAAGCATGGGACAAGAAGA′] 4654 NM_000531.5(OTC): c.905A > G 5009 OTC [′GCTGCCTCTGACTGGACATTTTTACDCTGC (p.His302Arg) TTGCCCAGAAAGCCAGAAGAA′] 4655 NM_000531.5(OTC): c.929A > G 5009 OTC [′CACTGCTTGCCCAGAAAGCCAGAAGRAGT (p.Glu310Gly) GGATGATGAAGTCTTTTATTCT′] 4656 NM_000531.5(OTC): c.988A > G 5009 OTC [′ACTAGTGTTCCCAGAGGCAGAAAACRGAA (p.Arg330Gly) AGTGGACAATCATGGTAAGCAA′] 4657 NM_000322.4(PRPH2): c.422A > G 5961 PRPH2 [′GGGCTCAAGAACGGCATGAAGTACTRCCG (p.Tyr141Cys) GGACACAGACACCCCTGGCAGG′] NM_003590.4(CUL3): c.1207 − 8452 CUL3 [] 26A > G NM_003590.4(CUL3): c.1376A > G 8452 CUL3 [] (p.Lys459Arg) NM_003590.4(CUL3): c.1377 + 3A > 8452 CUL3 [] G 4658 NM_017415.2(KLHL3): c.232A > G 26249 KLHL3 [′AGCCTGCAGCCCCTACTTCTGTGCGDTGTT (p.Met78Val) CACAGGTATGGTGAGTGGCCA] NM_017415.2(KLHL3): c.926A > G 26249 KLHL3 [] (p.Gln309Arg) 4659 NM_005763.3(AASS): c.874A > G 10157 AASS [′CTCACATCAGTATTAAAACGACTTAYGTA (p.Ile292Val) GCGCTCCGGATGTTTGTCATAC′] 4660 NM_000203.4(IDUA): c.1874A > G 3425 IDUA [′TCCTACCGAGTTCGAGCCCTGGACTRCTGG (p.Tyr625Cys) GCCCGACCAGGCCCCTTCTCG′] 4661 NM_000546.5(TP53): c.736A > G 7157 TP53 [′GTGTAACAGTTCCTGCATGGGCGGCVTGA (p.Met246Val) ACCGGAGGCCCATCCTCACCAT] 4662 NM_194442.2(LBR): c.1639A > G 3930 LBR [′GCCATGATGAGATCACCCAAGTAATYGGG (p.Asn547Asp) GTGGCGAACAAAGCCCCACCAT′] 4663 NM_000090.3(COL3A1): c.2284 − 1281 COL3A1 [′ATTAAAAAATATTTTTATTTCCTCTRGGGT 2A > G (p.Gly762_Lys779del) CCTACTGGTCCTATTGGTCCT′] 4664 NM_000090.3(COL3A1): c.997 − 1281 COL3A1 [′TCTTGAAATTGTATTTAATTTTTTCRGGGC 2A > G (p.Gly333_Lys350del+) CCTCCTGGTCCTCCTGGAACT′] 4665 NM_000090.3(COL3A1): c.3418 − 1281 COL3A1 [′CATTGTGATGTCATGATACTTTCTTDGGGA 2A > G CCTGTTGGACCCAGTGGACCT′] (p.Arg1139_Gly1140insVSSTERY YRSTCFRCLHFRKIFWHCDVMI LSW) 4666 NM_000090.3(COL3A1): c.997 − 1281 COL3A1 [′AAAAGAGCTCTTGAAATTGTATTTARTTTT 10A > G (p.Pro332_Gly333insFFQ) TTCAGGGCCCTCCTGGTCCTC′] 4667 NM_000090.3(COL3A1): c.1870 − 1281 COL3A1 [′ACAGAGTGTATCATTATACTTTTCTDGGGG 2A > G (p.Gly624_Gln641del) CCTGGTGGTGACAAAGGAGAC′] 4668 NM_000090.3(COL3A1): c.3202 − 1281 COL3A1 [′CTTCTCAATTGAATGTTTTCATCTTRGGGC 2A > G (p.Gly1068_Pro1085del) CCTGCTGGCCCTGCTGGTGCT′] 4669 NM_000090.3(COL3A1): c.1762 − 1281 COL3A1 [′AGTAAATACCGACCACTTCTTCTTTRGGGT 2A > G (p.Gly588_Gln605del) GCTCCTGGTAAGAATGGAGAA′] 4670 NM_003611.2(OFD1): c.935 + 706A > 8481 OFD1 [′CTTTTTGGCAATAATGAAAAGGTAARTTGA G TCAAGAGAGAGGAAATAGGCA′] 4671 NM_014740.3(EIF4A3): c.809A > G 9775 EIF4A3 [′AGTGATGGTCAGTGTGTCGTAGAGGYCAC (p.Asp270Gly) ACAGAGTGTCAAATTTCCACTC′] 4672 NM_001040436.2(YARS2): c.1303A > 51067 YARS2 [′TTTGTTACTTGTTGGTGATTTATGCYGACT G (p.Ser435Gly) CCGCCTTCTGTTATCATTCGA′] 4673 NM_000277.1(PAH): c.1157A > G 5053 PAH [′ACTGTCACGGAGTTCCAGCCCCTCTRTTAC (p.Tyr386Cys) GTGGCAGAGAGTTTTAATGAT′] 4674 NM_000277.1(PAH): c.812A > G 5053 PAH [′TTCCACTGCACACAGTACATCAGACDTGG (p.His271Arg) ATCCAAGCCCATGTATACCCCC′] 4675 NM_004820.3(CYP7B1): c.889A > G 9420 CYP7B1 [′ATTGCCCAGAACATAGTTGGAATAGYGTT (p.Thr297Ala) TGCCACAGAGGCCCAGAGAAAG′] 4676 NM_024301.4(FKRP): c.1A > G 79147 FKRP [′CCAGCTAGCCCCAGACTTCGGCCCCRTGCG (p.Met1Val) GCTCACCCGCTGCCAGGCTGC′] 4677 NM_001955.4(EDN1): c.271A > G 1906 EDN1 [′GTATGGACTTGGAAGCCCTAGGTCCRAGA (p.Lys91Glu) GAGCCTTGGAGAATTTACTTCC′] 4678 NM_198947.3(FAM111B): c.1879A > 374393 FAM111B [′TAATGTATACTGTATGTTTACCCAARGAAG G (p.Arg627Gly) TTTCCTATCAGAGGTTTGGAA′] 4679 NM_001282227.1(CECR1): c.1232A > 51816 CECR1 [′GCCCATGAAGACCTCATAGAAATCAYAGG G (p.Tyr411Cys) ACAAGCCTTTGGCACCAAACAT′] 4680 NM_033419.4(PGAP3): c.914A > G 93210 PGAP3 [′TGATTCCTTCAGCAGGTACAGGCTGYCATC (p.Asp305Gly) TTCCAGAAAGCTGTGGGCCAA′] 4681 NM_007294.3(BRCA1): c.135 − 672 BRCA1 [′TGTTCTTTCTTTCTTTATAATTTATRGATTT 2A > G TGCATGCTGAAACTTCTCAA′] 4682 NM_007294.3(BRCA1): c.4676 − 672 BRCA1 [′AAATTAAACTTCTCCCATTCCTTTCRGAGG 2A > G GAACCCCTTACCTGGAATCTG′] 4683 NM_015474.3(SAMHD1): c.1153A > 25939 SAMHD1 [′GGAAATGACAATCAAGTTTCTTACAYTGT G (p.Met385Val) ATCAATAATGTTGCCAACTTTG′] 4684 NM_015474.3(SAMHD1): c.1411 − 25939 SAMHD1 [′TTTTGGAAGAGATTCATAGTCCTCCYGGAA 2A > G AACACAAGACTCCCCATGTTA′] 4685 NM_000097.5(CPDX): c.980A > G 1371 CPDX [′ACCAATGCCCCGCCGTTCTCCACGAYGGG (p.His327Arg) CTATAAAGAAGTAATCATCACA′] 4686 NM_012338.3(TSPAN12): c.413A > 23554 TSPAN12 [′AAGCCACCGATATCTAGGTAATCCAYAAT G (p.Tyr138Cys) TTGTCATCCTGGCTTTCAAAGT′] 4687 NM_000406.2(GNRHR): c.94A > G 2798 GNRHR [′GTCACTCGGATCTTTCCAGACAAGGYCAG (p.Thr32Ala) AGTGGGGAGGTTGCCCTGCATC′] 4688 NM_199189.2(MATR3): c.1864A > 9782 MATR3 [′TGGTTCCCAGAAGACTGAGAGTTCARCCG G (p.Thr622Ala) AAGGTAAAGAACAAGAAGAGAA′] 4689 NM_003181.3(T): c.512A > G 6862 T [′CTGTGGACCCCCAACTCTCACTATGYGGAT (p.His171Arg) TCGAGGCTCATACTTATGCAA′] 4690 NM_000321.2(RB1): c.1927A > G 5925 RB1 [′AGCCTTCCAGACCCAGAAGCCATTGRAAT (p.Lys643 Glu) CTACCTCTCTTTCACTGTTTTA′] 4691 NM_002234.3(KCNA5): c.143A > G 3741 KCNA5 [′GCTGGGCTCAGCGATGGGCCCAAGGRGCC (p.Glu48Gly) GGCGCCAAAGGGGCGCGGCGCG′] 4692 NM_178014.3(TUBB): c.895A > G 203068 TUBB [′CCAGCAGGTCTTCGATGCCAAGAACRTGA (p.Met299Val) TGGCTGCCTGTGACCCCCGCCM 4693 NM_000136.2(FANCC): c.-78 − 2176 FANCC [′TGCTTCTATTTGTTCCCTTTCTTACRGATTT 2A > G AATGTGTGCCGACCATTTCC′] 4694 NM_000546.5(TP53): c.488A > G 7157 TP53 [′CTCCGTCATGTGCTGTGACTGCTTGYAGAT (p.Tyr163Cys) GGCCATGGCGCGGACGCGGGT′] 4695 NM_000546.5(TP53): c.659A > G 7157 TP53 [′TTTCGACATAGTGTGGTGGTGCCCTVTGAG (p.Tyr220Cys) CCGCCTGAGGTCTGGTTTGCA′] 4696 NM_000546.5(TP53): c.701A > G 7157 TP53 [′GGCTCTGACTGTACCACCATCCACTRCAAC (p.Tyr234Cys) TACATGTGTAACAGTTCCTGC′] 4697 NM_002878.3(RAD51D): c.1A > G −1 — [′CACAGTCCGACCCTGAGCACGCCCAYGTT (p.Met1Val) CCCCGCAGGCCGGAACAGCCCC′] 4698 NM_058216.2(RAD51C): c.706 − 5889 RAD51C [′ATCTAATATTATCTCTTCTGTATTTRGGTTC 2A > G GACTAGTGATAGTGGATGGT′] 4699 NM_015713.4(RRM2B): c.556A > G 50484 RRM2B [′TCTACAGCAGCAAAGGCCACCACTCYTTC (p.Arg186Gly) CCCTGGGAGACATAAAATCGTT′] 4700 NM_015713.4(RRM2B): c.581A > G 50484 RRM2B [′AAAAGATCCTGAGAAGAAAACTCCTYCTA (p.Glu194Gly) CAGCAGCAAAGGCCACCACTCT′] 4701 NM_000219.5(KCNE1): c.242A > G 3753 KCNE1 [′CACTCGAACGACCCATTCAACGTCTDCATC (p.Tyr81Cys) GAGTCCGATGCCTGGCAAGAG′] 4702 NM_000271.4(NPC1): c.1832A > G 4864 NPC1 [′TTCACTGCTGAACGAAGTATTGAAGRTGA (p.Asp611Gly) ACTAAATCGTGAAAGTGACAGT′] 4703 NM_000257.3(MYH7): c.1952A > G 4625 MYH7 [′CTGGGGCTGTGTCCCACTCACCCTGYGCAG (p.His651Arg) AGCTGACACAGTCTGAAAGGA′] 4704 NM_000540.2(RYR1): c.7043A > G 6261 RYR1 [′GGGTGGCCAGGCGAGAGCGTGGAGGRGA (p.Glu2348Gly) ACGCCAATGTGGTGGTGCGGCTG′] 4705 NM_058216.2(RAD51C): c.1027 − 5889 RAD51C [′TTTGTATATATATTTTTTATCTTTCRGCCTC 2A > G AGGGATTTAGAGATACTGTT′] 4706 NM_130838.1(UBE3A): c.1694 − 7337 UBE3A [′TGTATTTTTAAAAATCATTTCTTATRGGTA 2A > G TGTTCACATACGATGAATCTA′] 4707 NM_022168.3(IFIH1): c.1009A > G 64135 IFIH1 [′TCCTTGGCAATGTAAACAGCCACTCYGGTT (p.Arg337Gly) TTTCCACTCCCTGTAGGGAGG′] 4708 NM_022068.3(PIEZO2): c.2134A > 63895 PIEZO2 [′GCCACACAGAACAGGAACAGCACCAYGTA G (p.Met712Val) GATGATTTTGTACATTACGATT′] 4709 NM_001164342.2(ZBTB20): c.1787A > 26137 ZBTB20 [′ACCGCCAAACAGAACTACGTCAAGCRCAT G (p.His596Arg) GTTCGTACACACAGGTGAGTGT′] 4710 NM_001128159.2(VPS53): c.2084A > 55275 VPS53 [′TGCCTGATACTACGTCCATCTCACCYGTTC G (p.Gln695Arg) TGCTCCCACCATGCTAATTGG′] 4711 NM_003108.3(SOX11): c.347A > G 6664 SOX11 [′AAGCACATGGCCGACTACCCCGACTRCAA (p.Tyr116Cys) GTACCGGCCCCGGAAAAAGCCC′] 4712 NM_020435.3(GJC2): c.-170A > G 57165 GJC2 [′TTCAGACCCTGAGGCCGAGGGGGGARCAA TGGGGCCCTTGAGGGCCCCTCC′] 4713 NM_004817.3(TJP2): c.1992 − 2A > G 9414 TJP2 [′GATTTACTTCCCGTGGTTTCTTCTCRGAGC TGAACAAATGGCCAGTGTTCA′] 4714 NM_017565.3(FAM20A): c.720 − −1 — [′GGTGTCTCCTCATCTCGCTGCTGTCYGGAA 2A > G GGAAGGAAGGAATCACGCCCT′] 4715 NM_002764.3(PRPS1): c.343A > G 5631 PRPS1 [′AATCTCAGCCAAGCTTGTTGCAAATRTGCT (p.Met115Val) ATCTGTAGCAGGTGCAGATCA′] 4716 NM_000216.2(ANOS1): c.1A > G 3730 ANOS1 [′TCGCCCTCGCCCTCGACCCGCAGCCRTGGT (p.Met1Val) GCCCGGGGTGCCCGGCGCGGT′] 4717 NM_031229.2(RBCK1): c.1160A > 10616 RBCK1 [′TGGTGCTTCTTTGAGGATGATGTCARTGAG G (p.Asn387Ser) TTCACCTGCCCTGTGTGTTTC′] 4718 NM_015599.2(PGM3): c.737A > G 5238 PGM3 [′CACAAAGTCAGCTCCACATAAATGAYTGA (p.Asn246Ser) GTTTGCCCTTGGACCCATCATT′] 4719 NM_015599.2(PGM3): c.1352A > G 5238 PGM3 [′TTTCTCTCACACCTGAACTTTAAGTYGTCT (p.Gln451Arg) GTTTGGAAGATCTGTATAGAG′] 4720 NM_000535.5(PMS2): c.904 − 2A > G 5395 PMS2 [′CTTTTCTACTCCTTGTATTTTGTGCRGGTCT GCAGACTCGTGAATGAGGTC′] 4721 NM_000546.5(TP53): c.358A > G 7157 TP53 [′GGGCTTCTTGCATTCTGGGACAGCCDAGTC (p.Lys120Glu) TGTGACTTGCACGGTCAGTTG′] 4722 NM_000546.5(TP53): c.842A > G 7157 TP53 [′CGTGTTTGTGCCTGTCCTGGGAGAGDCCGG (p.Asp281Gly) CGCACAGAGGAAGAGAATCTC′] 4723 NM_001128425.1(MUTYH): c.1187 − 4595 MUTYH [′CCCCTGCCTGGCTGCCCTCCCTCTCRGGTC 2A > G TGCTGGCAGGACTGTGGGAGT′] 4724 NM_000546.5(TP53): c.1101 − 2A > G  7157 TP53 [′TCTCCTCCCTGCTTCTGTCTCCTACRGCCAC CTGAAGTCCAAAAAGGGTCA′] 4725 NM_000314.6(PTEN): c.493  − 2A > G 5728 PTEN [′GGCTTCTCTTTTTTTTCTGTCCACCRGGGA GTAACTATTCCCAGTCAGAGG′] 4726 NM_000051.3(ATM): c.3994 − 2A > G 472 ATM [′ATATATTTTAATTTTGTGCCCTTGCRGATT GATCACTTATTCATTAGTAAT′] 4727 NM_000314.6(PTEN): c.403A > G 5728 PTEN [′TGGAAAGGGACGAACTGGTGTAATGRTAT (p.Ile135Val) GTGCATATTTATTACATCGGGG′] 4728 NM_000249.3(MLH1): c.117 − 2A > G 4292 MLH1 [′TAAATTATTTTCTGTTTGATTTGCCDGTTTA GATGCAAAATCCACAAGTAT′] 4729 NM_025150.4(TARS2): c.695 + 3A > 80222 TARS2 [′GGTCCAACAGCAACAGTATATGGGTRAGA G GTTGTCAAGATTAAGGCAAACA′] 4730 NM_206933.2(USH2A): c.10544A > 7399 USH2A [′TGGACCAAAATAGACAATCTTGAAGVTAC G (p.Asp3515Gly) AATTGTCTTAAACTGGAGAAAA′] 4731 NM_000834.3(GRIN2B): c.1238A > 2904 GRIN2B [′CATCTGAGCATTGTGACCCTGGAGGRGGC G (p.Glu413Gly) ACCATTTGTCATTGTGGAAAGT′] 4732 NM_004830.3(MED23): c.3638A > 9439 MED23 [′TGTAGCTATACGTTAGCTCTTGCACRTGCT G (p.His1213Arg) GTGTGGCACCATTCTAGCATC′] 4733 NM_003159.2(CDKL5): c.125A > G 6792 CDKL5 [′GAAACACATGAAATTGTGGCGATCARGAA (p.Lys42Arg) ATTCAAGGACAGTGAAGGTAGA′] 4734 NM_003159.2(CDKL5): c.380A > G 6792 CDKL5 [′CTAATCAAGGCTATTCACTGGTGCCRTAAG (p.His127Arg) AATGATATTGTCCATCGAGGT′] 4735 NM_003159.2(CDKL5): c.578A > G 6792 CDKL5 [′AGCGCTCCCTATGGAAAGTCCGTGGRCAT (p.Asp193Gly) GTGGTCGGTGGGCTGTATTCTT′] 4736 NM_198282.3(TMEM173): c.461A < 340061 TMEM173 [′TGACCATGCCAGCCCATGGGCCACGYTGA G (p.Asn154Ser) AATTCCCTTTTTCACACACTGC′] 4737 NM_032018.6(SPRTN): c.350A > G 83932 SPRTN [′CTCCTGCATGAAATGATACATGCCTRTTTA (p.Tyr117Cys) TTTGTCACTAATAACGACAAA′] 4738 NM_001759.3(CCND2): c.838A > G 894 CCND2 [′GGAGGATGAACTGGACCAAGCCAGCRCCC (p.Thr280Ala) CTACAGACGTGCGGGATATCGA′] 4739 NM_007315.3(STAT1): c.854A > G 6772 STAT1 [′AGGGTCATGTTCGTAGGTGTATTTCYGTTC (p.Gln285Arg) CAATTCCTCCAACTTTTTAAG′] 4740 NM_139276.2(STAT3): c.1175A > G 6774 STAT3 [′GTTGGATTCTTCCATGTTCATCACTYTTGT (p.Lys392Arg) GTTTGTGCCCAGAATGTTAAA′] 4741 NM_001037811.2(HSD17B10): c.257A > 3028 HSD17B10 [′GATGCCTGCACAGTTGACAGCTACAYCCA G (p.Asp86Gly) CACGGCCAAACTTTCCTTTTGC′] 4742 NM_005726.5(TSFM): c.57 + 4A > G 10102 TSFM [′CGCGCGGACCGGGAGCTACCCGGTGRGAA GTCCTGGTGCTGGTACCGACCT′] 4743 NM_000166.5(GJB1): c.580A > G 2705 GJB1 [′CGAGAAAACCGTCTTCACCGTCTTCRTGCT (p.Met194Val) AGCTGCCTCTGGCATCTGCAT′] 4744 NM_001077494.3(NFKB2): c.2594A > 4791 NFKB2 [′TTACCCCCAGCAGAGGTGAAGGAAGRCAG G (p.Asp865Gly) TGCGTACGGGAGCCAGTCAGTG′] 4745 NC_012920.1: m.5559A > G 4578 MT-TW [′TGTTACAGAAATTAAGTATTGCAACYTACT GAGGGCTTTGAAGGCTCTTGG′] 4746 NC_012920.1: m.13514A > G 4540 MT-NDS [′TTCCTCACAGGTTTCTACTCCAAAGRCCAC ATCATCGAAACCGCAAACATA′] 4747 NM_007315.3(STAT1): c.2018A > G 6772 STAT1 [′GTAATACTTTCCAAAGGCATGGTCTYTGTC (p.Lys673Arg) AATATTTGGATACAGATACTT′] 4748 NM_007315.3(STAT1): c.1909A > G 6772 STAT1 [′GGGAAAGTAACAGCAGAAAGTTCTTYCTT (p.Lys637 Glu) CGTGTAGGGTTCAACCGCATGG′] 4749 NM_000060.3(BTD): c.683A > G 686 BTD [′GCATTCGATGTTCCTCTTAAAGTGGRTCTC (p.Asp228Gly) ATCACCTTTGATACCCCCTTT′] 4750 NM_004992.3(MECP2): c.27 − 2A > G 4204 MECP2 [′TACTTACATACTTGTTTAACACTTCRGGGA AGAAAAGTCAGAAGACCAGGA′] 4751 NM_004992.3(MECP2): c.378 − 4204 MECP2 [′CTTGTGTCTTTCTGTTTGTCCCCACNGTCCC 2A > G CAGGGAAAAGCCTTTCGCTC′] 4752 NM_003159.2(CDKL5): c.100 − 6792 CDKL5 [′TTTCCTTCTGCTTCTTTTCCCTTGCRGGAAA 2A > G CACATGAAATTGTGGCGATC′] 4753 NM_003159.2(CDKL5): c.464 − 6792 CDKL5 [′ACAACTTTGGACTTTGCTATCTTTCRGGTT 2A > G TTGCTCGTAATCTGTCAGAAG′] 4754 NM_003159.2(CDKL5): c.978 − 6792 CDKL5 [′GATATACTTCTTTTGTTTTTAACATRGAAA 2A > G CCAAGCCGGCAAAAGTACTGC′] 4755 NM_032492.3(JAGN1): c.485A > G 84522 JAGN1 [′TTGGCAGTGCAAGTGCATGCCTGGCRGTTG (p.Gln162Arg) TACTACAGCAAGAAGCTCCTA′] 4756 NM_178517.3(PIGW): c.499A > G 284098 PIGW [′AACTGAGCTCTATGGGACAGGAGCARTGG (p.Met167Val) ATTTTGGAGTAGGTGGCTTTGT′] 4757 NM_004387.3(NKX2-5): c.461A > G 1482 NKX2-5 [′TCGCAGGCGCAGGTCTATGAGCTGGRGCG (p.Glu154Gly) GCGCTTCAAGCAGCAGCGGTAC′] 4758 NM_005859.4(PURA): c.289A > G 5813 PURA [′CGCCGAGGTGGGCGCGGGCGGCAACRAGA (p.Lys97Glu) GCCGCCTTACTCTCTCCATGTC′] 4759 NM_004046.5(ATP5A1): c.962A > G 498 ATP5A1 [′GCGGAGCAACAGAGACATCTGACGGHAAG (p.Tyr321Cys) CAACAGCCTATGGTACAGAATA′] 4760 NM_000038.5(APC): c.1744 − 2A > G 324 APC [′GATGACCCATATTCTGTTTCTTACTRGGAA TCAACCCTCAAAAGCGTATTG′] 4761 NM_000751.2(CHRND): c.1319A > 1144 CHRND [′TTCAATGAGCTGAAGCCAGCTGTGGWTGG G (p.Asp440Gly) GGCAAACTTCATTGTTAACCAC′] 4762 NM_130466.3(UBE3B): c.1A > G 89910 UBE3B [′GCAGGGTTTGTGCAAGTTTGCAAACRTGTT (p.Met1Val) CACCCTGTCTCAGACCTCGAG′] 4763 NM_003159.2(CDKL5): c.211A > G 6792 CDKL5 [′AATGCTTCGGACTCTCAAGCAGGAARACA (p.Asn7lAsp) TTGTGGAGTTGAAGGAAGCATT′] 4764 NM_003159.2(CDKL5): c.404 − 6792 CDKL5 [′AATTTTTTTTTTATCTTGACACTCCRGATAT 2A > G AAAACCAGAAAATCTCTTAA′] 4765 NM_003159.2(CDKL5): c.449A > G 6792 CDKL5 [′TTAATCAGCCACAATGATGTCCTAARACTG (p.Lys150Arg) TGTGACTTTGGTAAGTTAAAA′] 4766 NM_001813.2(CENPE): c.4063A > G 1062 CENPE [′ACTTCAAGGGCTTCTTTTATCGTTTYAAGG (p.Lys1355Glu) TTGTCTCTTTCCTTGGTTAGA′] 4767 NM_023073.3(C5orf42): c.3290 − 65250 C5orf42 [′ATTTTACCAAATTTTTGTATTTGATRGATC 2A > G CCATTGAAGAGGAAGATGCAA′] 4768 NM_002977.3(SCN9A): c.2215A > G −1 — [′GTGTTTAAAACTATGCAAATGGTAAYTGC (p.Ile739Val) AAGATCTACAAAAGGATCCATT′] 4769 NM_002354.2(EPCAM): c.556 − 4072 EPCAM [′TATTAGTATTAATTTGTATTATTCANTTTTT 14A > G TTCCCCAGTATGAGAATAAT′] 4770 NM_002354.2(EPCAM): c.492 − 4072 EPCAM [′TTAATACAGATTTTAAATTCTTTACRGTGC 2A > G ACTTCAGAAGGAGATCACAAC′] 4771 NM_000053.3(ATP7B): c.122A > G 540 ATP7B [′ATCCAGACCACCTTCATAGCCAACAYTGTC (p.Asn41Ser) AAAAGCAAAACTCTTCTTCAT′] 4772 NM_022455.4(NSD1): c.4498 − 64324 NSD1 [′ATGTGGGACATTATTTTTTCTTTGCRAGGG 3A > G AGAACTAATGCCTCACAGGAC′] 4773 NM_022455.4(NSD1): c.5893 − 64324 NSD1 [′TAAGTCAGGAGGTATTTCTTGTTCTRGGGT 2A > G GAATTTGTGAATGAGTATGTG′] 4774 NM_022455.4(NSD1): c.6059A > G 64324 NSD1 [′AAAGGAAACTATGCTCGGTTCATGARTCA (p.Asn2020Ser) TTGCTGCCAGCCCAACTGTGAA′] 4775 NM_022455.4(NSD1): c.6356A > G 64324 NSD1 [′GGTGAAATCACAAAGGAGCGAGAAGRTGA (p.Asp2119Gly) GTGTTTTAGTTGTGGGGATGCT′] 4776 NM_133433.3(NIPBL): c.737A > G 25836 NIPBL [′AATCCTAGACATGGTTCAAGTGAGGRCTA (p.Asp246 Gly) CCTACACATGGTGCACAGGCTA′] 4777 NM_133433.3(NIPBL): c.5428 − 25836 NIPBL [′TGTTTTTTTCTCTTCATTTTTCTTTRGCTTG 2A > G ATATGCAACGAGGTGTTCAT′] 4778 NM_005249.4(FOXG1): c.757A > G 2290 FOXG1 [′CCACTACGACGACCCGGGCAAGGGCRACT (p.Asn253Asp) ACTGGATGCTGGACCCGTCGAG′] 4779 NM_004380.2(CREBBP): c.4508A > 1387 CREBBP [′AAGCCAAAACGACTGCAGGAGTGGTRCAA G (p.Tyr1503Cys) AAAGATGCTGGACAAGGCGTTT′] 4780 NM_004380.2(CREBBP): c.3983 − 1387 CREBBP [′CTCACCTGCTCCTTCTGGACTTCCTRGGGC 2A > G TGCAGACCACAAGACTGGGAA′] 4781 NM_001083962.1(TCF4): c.991 − 6925 TCF4 [′TTTTTTTTTTCTCCTTATTGTTTTARGATCT 2A > G ATTCTCCAGATCACACTAAC′] 4782 NM_178151.2(DCX): c.1027 − 2A > G 1641 DCX [′TTAACTTTGTCTCTTCTCTTCTTATRGGACC TGTACCTGCCTCTGTCCTTG′] 4783 NM_178151.2(DCX): c.607A > G 1641 DCX [′TGTGCGTGTGCTTCTGAACAAGAAGRCAG (p.Thr203Ala) CCCACTCTTTTGAGCAAGTCCT′] 4784 NM_178151.2(DCX): c.538A > G 1641 DCX [′GGAGAACAAGGACTTTGTGCGCCCCRAGC (p.Lys180Glu) TGGTTACCATCATCCGCAGTGG′] 4785 NM_178151.2(DCX): c.520A > G 1641 DCX [′CAACAGTGCACAGGCCAGGGAGAACRAGG (p.Lys174Glu) ACTTTGTGCGCCCCAAGCTGGT′] 4786 NM_178151.2(DCX): c.451A > G 1641 DCX [′CAATCCCAACTGGTCTGTCAACGTADAAA (p.Lys151Glu) CATCTGCCAATATGAAAGCCCC′] 4787 NM_178151.2(DCX): c.413A > G 1641 DCX [′GACAACTTCTTTAAAAAGGTGGAGTRCAC (p.Tyr138Cys) CAAGAATGTCAATCCCAACTGG′] 4788 NM_000252.2(MTM1): c.301A > G 4534 MTM1 [′AATTGAAAAAATGGGAGGCGCGACARGTA (p.Ser101Gly) GAGGAGAAAATTCCTATGGTCT′] 4789 NM_000252.2(MTM1): c.343 − 4534 MTM1 [′ACTGTCATACTTCTCCTTTGCCCCCRGGAC 2A > G ATGAGAAACCTGAGGTTCGCT′] 4790 NM_000252.2(MTM1): c.529 − 4534 MTM1 [′GACTTGAATTTCTTTTTTTCCTCACRGGGCT 2A > G TGCCCAATCACCATTGGAGA′] 4791 NM_000252.2(MTM1): c.550A > G 4534 MTM1 [′ACAGGGCTTGCCCAATCACCATTGGRGAA (p.Arg184Gly) TAACTTTTATTAATAAGTGCTA′] 4792 NM_000252.2(MTM1): c.629A > G 4534 MTM1 [′TTGGTGGTTCCGTATCGTGCCTCAGRTGAT (p.Asp210Gly) GACCTCCGGAGAGTTGCAACT′] 4793 NM_000252.2(MTM1): c.1406A > G 4534 MTM1 [′CAATTTTTGATTATAATTTTGGATCRTCTGT (p.His469Arg) ATAGTTGCCGATTTGGTACT′] 4794 NM_003159.2(CDKL5): c.62A > G 6792 CDKL5 [′TTTGAGATCCTTGGGGTTGTAGGTGRAGGT (p.Glu21Gly) AAGTTGGAATTTTTGCGTTCC′] 4795 NM_006306.3(SMC1A): c.3254A > 8243 SMC1A [′GTGGCTACCAACATTGATGAGATCTRTAA G (p.Tyr1085Cys) GGCCCTGTCCCGCAATAGCAGT′] 4796 NM_006306.3(SMC1A): c.616 − 8243 SMC1A [′CCTGCCACCATTCCCCTGTTTGCACRGGCT 2A > G GACCGGTACCAGCGCCTGAAG′] 4797 NM_003937.2(KYNU): c.592A > G 8942 KYNU [′GCCTAACTTGATTTAGGGGGAAGAARCCT (p.Thr198Ala) TAAGAATAGAGGATATCCTTGA′] 4798 NM_005343.2(HRAS): c.182A > G −1 — [′TTGGACATCCTGGATACCGCCGGCCNGGA (p.Gln6lArg) GGAGTACAGCGCCATGCGGGAC′] 4799 NM_012275.2(IL36RN): c.104A > G 26525 IL36RN [′CTAGCTGGAGGGCTGCATGCAGGGARGGT (p.Lys35Arg) CATTAAAGGTTGGTGATGAAAC′] 4800 NM_152296.4(ATP1A3): c.2318A > 478 ATP1A3 [′TCCATTGCCTACACCCTGACCAGCADTATC G (p.Asn773Ser) CCGGAGATCACGCCCTTCCTG′] 4801 NM_000891.2(KCNJ2): c.953A > G 3759 KCNJ2 [′CAGTGCCGTAGCTCTTATCTAGCAARTGAA (p.Asn318Ser) ATCCTGTGGGGCCACCGCTAT] 4802 NM_017909.3(RMND1): c.713A > G 55005 RMND1 [′TACACTTACAGTTTTGTCTTTCACAYTCCA (p.Asn238Ser) AAACACAGCAGCTCCTTCCCT] 4803 NM_005154.4(USP8): c.2150A > G 9101 USP8 [′GAACCTTCCAAACTGAAGCGCTCCTDCTCC (p.Tyr717Cys) TCCCCAGATATAACCCAGGCT′] 4804 NM_014191.3(SCN8A): c.667A > G 6334 SCN8A [′CAATGTTTCAGCTCTACGCACTTTCRGGGT (p.Arg223Gly) ACTGAGGGCTTTGAAAACTAT′] 4805 NM_021830.4(C10orf2): c.1754A > 56652 C10orf2 [′CCCCAGGCAAGCCAGGAAGCAGACARTGT G (p.Asn585Ser) TCTGATCCTGCAGGACAGGAAG′] 4806 NM_001164405.1(BHLHA9): c.211A > 727857 BHLHA9 [′GTCCAAGGCGCGGCGCATGGCCGCCRACG G (p.Asn7lAsp) TGCGGGAGCGCAAGCGCATCCT′] 4807 NM_002887.3(RARS): c.5A > G 5917 RARS [′GAGTGAGACGCTGATGGGAGGATGGRCGT (p.Asp2 Gly) ACTGGTGTCTGAGTGCTCCGCG′] 4808 NM_002887.3(RARS): c.1A > G 5917 RARS [′TGGCGAGTGAGACGCTGATGGGAGGRTGG (p.Met1Val) ACGTACTGGTGTCTGAGTGCTC′] 4809 NM_005861.3(STUB1): c.194A > G 10273 STUB1 [′CCGCTGGTGGCCGTGTATTACACCARCCGG (p.Asn65Ser) GCCTTGTGCTACCTGAAGATG′] 4810 NM_005211.3(CSF1R): c.2655 − 1436 CSF1R [′ATGGGCATCCTCTGTCCTATCTCCCRGATA 2A > G CAGCATCATGCAGGCCTGCTG′] 4811 NM_032228.5(FAR1): c.1094A > G 84188 FAR1 [′CATAAGGCCCCAGCATTCCTGTATGRTATC (p.Asp365Gly) TACCTCAGGATGACTGGAAGA′]   4812 NM_020461.3(TUBGCP6): c.2546A > 85378 TUBGCP6 [′GCCATCCCAGGCAGGCGAGTGTTGCYCTG G (p.Glu849Gly) CAGACCCAGAATCACAGCCTTG′] 4813 NM_014305.3(TGDS): c.269A > G 23483 TGDS [′TTTGTGAAACTGCTTTTTGAAACAGRGAAA (p.Glu90Gly) ATAGATATAGTACTACATTTT′] 4814 NM_014305.3(TGDS): c.892A > G 23483 TGDS [′TCTGTTTTGTTTTCCAAGACCCACCRATGA (p.Asn298Asp) CATGAGATACCCAATGAAGTC′] 4815 NM_000118.3(ENG): c.1273 − 2A > G 2022 ENG [′GCTCGACAGGATATTGACCACCGCCYGCG GGGATAAAGCCAGGGAGCTGGT′] 4816 NM_005360.4(MAF): c.172A > G 4094 MAF [′CATCGCCGGGGGCTCGCTGTCCTCCRCCCC (p.Thr58Ala) CATGAGCACGCCGTGCAGCTC′] 4817 NM_002238.3(KCNH1): c.1399A > 3756 KCNH1 [′ACCTCTAGCACTTCTCTATGCCACCRTCTT G (p.Ile467Val) CGGGAATGTGACGACTATTTT′] 4818 NM_172362.2(KCNH1): c.1508A > 3756 KCNH1 [′TTCGGGAATGTGACGACTATTTTCCRACAG G (p.Gln503Arg) ATGTATGCCAACACCAACAGA′] 4819 NM_003392.4(WNT5A): c.257A > G 7474 WNT5A [′GGACAGAAGAAACTGTGCCACTTGTRTCA (p.Tyr86Cys) GGACCACATGCAGTACATCGGA′] 4820 NM_001199252.2(SGOL1): c.67A > −1 — [GCCAAGTTTTTATTCCTTTTCTCTTYCATTC G (p.Lys23Glu) GCTTCTTTATGTCTTCAAGA′] 4821 NM_000256.3(MYBPC3): c.2906 − 4607 MYBPC3 [′ACTTAGCTACCCACTCTATACCCACRGAAC 2A > G GGCCACGGCTTCAGCTGCCCA′] 4822 NM_000256.3(MYBPC3): c.1213A > 4607 MYBPC3 [′GCTCAAGAATGGCCAGGAGATCCAGRTGA G (p.Met405Val) GCGGCAGGTGCAGCCTGGGGTG′] 4823 NM_005188.3(CBL): c.1228 − 2A > G 867 CBL [′TGTTACTATCTTTTGCTTCTTCTGCRGGAAT CAGAAGGTCAGGGCTGTCCT′] 4824 NM_000257.3(MYH7): c.5326A > G 4625 MYH7 [′TTCTTCATGCGCTCCAGGTGGGCGCYGGTG (p.Ser1776Gly) TCCTGCTCCTTCTTCAGCTCC′] 4825 NM_000257.3(MYH7): c.2708A > G 4625 MYH7 [′CAAGACAACCTGGCAGATGCTGAGGRGCG (p.Glu903Gly) CTGTGATCAGCTGATCAAAAAC′] 4826 NM_000257.3(MYH7): c.1727A > G 4625 MYH7 [′AATATCAAGGGGAAGCCTGAAGCCCRCTT (p.His576Arg) CTCCCTGATCCACTATGCCGGC′] 4827 NM_000257.3(MYH7): c.1496A > G 4625 MYH7 [′CACCACATGTTTGTGCTGGAGCAGGRGGA (p.Glu499Gly) GTACAAGAAGGAGGGCATCGAG′] 4828 NM_000257.3(MYH7): c.2539A > G 4625 MYH7 [′GCTGCTGAAGAGTGCAGAAAGAGAGRAGG (p.Lys847Glu) AGATGGCCTCCATGAAGGAGGA′] 4829 NM_000257.3(MYH7): c.1954A > G 4625 MYH7 [′CTTTCAGACTGTGTCAGCTCTGCACRGGGT (p.Arg652Gly) GAGTGGGACACAGCCCCAGCC′] 4830 NM_000257.3(MYH7): c.1157A > G 4625 MYH7 [′CTTCCAGAGGCTGACAAGTCTGCCTRCCTC (p.Tyr386Cys) ATGGGGCTGAACTCAGCCGAC′] 4831 NM_000501.3(ELN): c.800 − 2A > G 2006 ELN [′TACATTGCACTGTCCCCATCTCAACRGGTG CTGGAGCAGCCGGAGTCCTCC′] 4832 NM_000117.2(EMD): c.266 − 2A > G 2010 EMD [′TCTGCTACCGCTGCCCCCCTTCCCARGGCT ACAATGACGACTACTATGAAG′] 4833 NM_000191.2(HMGCL): c.698A > G 3155 HMGCL [′GTGCCTCTGGCTGCCCTGGCTGTCCRCTGC (p.His233Arg) CATGACACCTATGGTCAAGCC′] 4834 NM_207352.3(CYP4V2): c.1393A > 285440 CYP4V2 [′CTACGTGCCCTTCTCTGCTGGCCCCRGGAA G (p.Arg465Gly) CTGTATAGGTTTGTATCCATC′] 4835 NM_013382.5(POMT2): c.1726 − 29954 POMT2 [′GTCAGCAGGGTGGTCTCTATTCCACRGGGC 2A > G CTACGCTTCTCAGGGGTCAAT′] 4836 NM_000169.2(GLA): c.802 − 2A > G −1 — [′TTTGAATTATTTCATTCTTTTTCTCRGTTAG TGATTGGCAACTTTGGCCTC′] 4837 NM_020166.4(MCCC1): c.137 − 56922 MCCC1 [′GTGTGATTTTCATGGTGTTTTAAACRGGAA 2A > G GAAACATTACCAAGGTCCTCA′] 4838 NM_006920.4(SCN1A): c.2557 − 6323 SCN1A [′AATATATATTAATCTTTCATTTTCCRGCTG 2A > G CGAGTTTTCAAGTTGGCAAAA′] 4839 NM_000501.3(ELN): c.890 − 2A > G 2006 ELN [′CCTCACCCTCTGTGGCTGTGTTTTCRGGCG TTGGGACTCCAGCTGCAGCTG′] 4840 NM_006306.3(SMC1A): c.2974 − 8243 SMC1A [′TGACATTGCTGGGCCTGGGGCTTACRGGAT 2A > G GCCCAGGCTGAGGAAGAGATC′] 4841 NM_001110792.1(MECP2): c.520A > 4204 MECP2 [′TGATTTTGACTTCACGGTAACTGGGRGAGG G (p.Arg174Gly) GAGCCCCTCCCGGCGAGAGCA′] 4842 NM_004646.3(NPHS1): c.1756A > G 4868 NPHS1 [′CTTGTCCTGGGACAAGGAAGGGGAGRGGT (p.Arg586Gly) GGGAGTGCGAGGGATCCCTCCC′] 4843 NM_003002.3(SDHD): c.275A > G 6392 SDHD [′TATTTGAATCCTTGCTCTGCGATGGRCTAT (p.Asp92 Gly) TCCCTGGCTGCAGCCCTCACT′] 4844 NM_004793.3(LONP1): c.2353A > G 9361 LONP1 [′CACGCTGTTTGTGGAGACATCCCTGRGACG (p.Arg785Gly) GCCACAGGACAAGGATGCCAA′] 4845 NC_012920.1: m.4279A > G 4565 MT-TI [′TCAAACCTAAGAAATATGTCTGATARAAG AGTTACTTTGATAGAGTAAATA′] 4846 NM_005633.3(SOS1): c.1430A > G 6654 SOS1 [′ATTTGCTGTAAATCAAATCATGGGCRGCCA (p.Gln477Arg) AGACTTCCTGGTGCTAGCAAT′] 4847 NM_000256.3(MYBPC3): c.1227 − 4607 MYBPC3 [′TGCCACTTCCCTGCGGCCCCCACCCRGGTA 2A > G CATCTTTGAGTCCATCGGTGC′] 4848 NM_002834.3(PTPN11): c.661A > G 5781 PTPN11 [′CGATCAGCCCCTTAACACGACTCGTDTAA (p.Ile221Val) ATGCTGCTGAAATAGAAAGCAG′] 4849 NM_000257.3(MYH7): c.4664A > G −1 — [′TCCCAGGCCTCCCTGGAGCACGAGGRGGG (p.Glu1555Gly) CAAGATCCTCCGGGCCCAGCTG′] 4850 NM_000257.3(MYH7): c.2792A > G 4625 MYH7 [′AACGAGAGGCTGGAGGATGAGGAGGRGA (p.Glu931Gly) TGAATGCTGAGCTCACTGCCAAG′] 4851 NM_000257.3(MYH7): c.2087A > G 4625 MYH7 [′CTGGTCATGCACCAGCTGCGCTGCARTGGT (p.Asn696Ser) GTGCTGGAGGGCATCCGCATC′] 4852 NM_000257.3(MYH7): c.1805A > G 4625 MYH7 [′CTGCAGAAGAACAAGGATCCTCTCARTGA (p.Asn602Ser) GACTGTCGTGGGCTTGTATCAG′] 4853 NM_000257.3(MYH7): c.1615A > G 4625 MYH7 [′CATGTCCATCCTGGAAGAGGAGTGCRTGTT (p.Met539Val) CCCCAAGGCCACCGACATGAC′] 4854 NM_000257.3(MYH7): c.1477A > G 4625 MYH7 [′GCTGCAGCAGTTCTTCAACCACCACRTGTT (p.Met493Val) TGTGCTGGAGCAGGAGGAGTA′] 4855 NM_000257.3(MYH7): c.1331A > G 4625 MYH7 [′TTCAACTGGATGGTGACGCGCATCAVTGC (p.Asn444Ser) CACCCTGGAGACCAAGCAGCCA′] 4856 NM_000257.3(MYH7): c.1315A > G 4625 MYH7 [′AGGGTGGCATTGATGCGCGTCACCAHCCA (p.Met439Val) GTTGAACATCCTCTCATACACT′] 4857 NM_000257.3(MYH7): c.1051A > G 4625 MYH7 [′TTCAGAGGAGAAAAACTCCATGTATRAGC (p.Lys351Glu) TGACAGGCGCCATCATGCACTT′] 4858 NM_000257.3(MYH7): c.789A > G 4625 MYH7 [′CAGGAAAGTTGGCATCTGCAGACATRGAG (p.Ile263Met) ACCTGTGAGTGCCATGAATCTG′] 4859 NM_000257.3(MYH7): c.617A > G 4625 MYH7 [′ATTGCAGCCATTGGGGACCGCAGCARGAA (p.Lys206Arg) GGACCAGAGCCCGGGCAAGGTA′] 4860 NM_000363.4(TNNI3): c.616A > G 7137 TNNI3 [′ACTGAGTGGAATGGAGGGCCGCAAGVAAA (p.Lys206Glu) AGTTTGAGAGCTGAGCCTTCCT] 4861 NM_000363.4(TNNI3): c.547A > G 7137 TNNI3 [′GCAGGTGAAGAAGGAGGACACCGAGRAG (p.Lys183Glu) GTGAGTGTGGGCTAAGGCCAGGA′] 4862 NM_000169.2(GLA): c.370 − 2A > G −1 — [′TGACTCTTTTCCTCCCTCTCATTTCRGGTTC ACAGCAAAGGACTGAAGCTA′] 4863 NM_000051.3(ATM): c.1A > G 472 ATM [′GATGTGTGTTCTGAAATTGTGAACCVTGAG (p.Met1Val) TCTAGTACTTAATGATCTGCT′] 4864 NM_000051.3(ATM): c.3154 − 2A > G 472 ATM [′TATTTAACCACAGTTCTTTTCCCGTRGGCT GATCCTTATTCAAAATGGGCC′] 4865 NM_024675.3(PALB2): c.212 − 79728 PALB2 [′AGTTATATACATTTTTTTCCTCCTCRGAAC 2A > G CTAAAAATAAAATATGTGTTT′] 4866 NM_024675.3(PALB2): c.109 − 79728 PALB2 [′TTCTGGGGCTGTTTTTGTCTCCTCTRGCGTG 2A > G CCCAAAGAGCTGAAAAGATT′] 4867 NM_000546.5(TP53): c.709A > G 7157 TP53 [′CTGTACCACCATCCACTACAACTACRTGTG (p.Met237Val) TAACAGTTCCTGCATGGGCGG′] 4868 NM_000455.4(STK11): c.889A > G 6794 STK11 [′GATGCTTGAGTACGAACCGGCCAAGRGGT (p.Arg297Gly) TCTCCATCCGGCAGATCCGGCA′] 4869 NM_017777.3(MKS1): c.1382A > G 54903 MKS1 [′TCTCTGGAACTGGAGGACCTCTCCTRTGTA (p.Tyr461Cys) CGGATACCAGGATCCTTCAAG′] 4870 NM_001231.4(CASQ1): c.731A > G 844 CASQ1 [′ATGGAAGAGCCTGTGACCATCCCAGRCAA (p.Asp244Gly) GCCCAATAGCGAAGAGGAGATT′] 4871 NM_001242896.1(DEPDC5): c.2355 − 9681 DEPDC5 [′GTATGAGCAATCATCTGTTGTTTTCRGGAG 2A > G (p.Arg785_Gly839del) GGACGAAGATGGTGTGCAGAT′] 4872 NM_001039550.1(DNAJB2): c.14A > 3300 DNAJB2 [′TGACCAGTTGCCATGGCATCCTACTRCGAG G (p.Tyr5Cys) ATCCTAGACGTGCCGCGAAGT′] 4873 NM_007294.3(BRCA1): c.5057A > 672 BRCA1 [′AATCTAATTACTGAAGAGACTACTCRTGTT G (p.His1686Arg) GTTATGAAAACAGGTATACCA′] 4874 NM_006888.4(CALM1): c.389A > G 801 CALM1 [′GTAGATGAAATGATCAGAGAAGCAGRTAT (p.Asp130Gly) TGATGGAGACGGACAAGTCAAC′] 4875 NM_001082538.2(TCTN1): c.342 − 79600 TCTN1 [′TTGTATTATTATTTTTTTAATTTTCRGGGGC 2A > G GACAGCCAGTTTTGTAGTCA′] 4876 NM_003000.2(SDHB): c.541 − 2A > G 6390 SDHB [′TCCTGCCTCTCTTTTCTCCCCATACRGGAC GGGCTCTACGAGTGCATTCTC′] 4877 NM_000143.3(FH): c.700A > G 2271 FH [′AGTGGAACAGCATCCTGAGTATGAGYACG (p.Thr234Ala) TCCAATCTTGATGATCTGTGCA′] 4878 NM_000038.5(APC): c.221 − 2A > G 324 APC [′ATAAAAACTTGTTTCTATTTTATTTRGAGC TTAACTTAGATAGCAGTAATT′] 4879 NM_000314.6(PTEN): c.527A > G 5728 PTEN [′ATTCCCAGTCAGAGGCGCTATGTGTRTTAT (p.Tyr176Cys) TATAGCTACCTGTTAAAGAAT′] 4880 NM_030813.5(CLPB): c.1850A > G 81570 CLPB [′CTGGTCGACGGCTACAATGTGCACTRTGGC (p.Tyr617Cys) GCCCGCTCCATCAAACATGAG′] 4881 NM_030813.5(CLPB): c.1222A > G 81570 CLPB [′TCCTGGAACTCGGACATGTCCAGCCHGAT (p.Arg408Gly) GAAGCCCTGTGTGGAAACAAGC′] 4882 NM_016069.9(PAM16): c.226A > G −1 — [′CTCACCCGTCCCCTCTCCTCTGCAGRACTA (p.Asn76Asp) TGAACACTTATTTAAGGTGAA′] 4883 NM_058163.1(TSR2): c.191A > G 90121 TSR2 [′TACTTAGCTGACTTGGAGCTAGATGRGGTG (p.Glu64Gly) GAAGACTTCCTTGGAGAGCTG′] 4884 NM_001031.4(RPS28): c.1A > G 6234 RPS28 [′ATAGGCTGCACACGGCTGGTGTCCAYGAT (p.Met1Val) GGCGGCGCGGCGGCGGTCTGGC′] 4885 NM_005957.4(MTHFR): c.1114A > 4524 MTHFR [′TCCCATCTTCTGGGCCTCCAGACCARAGAG G (p.Lys372Glu) TTACATCTACCGTACCCAGGA′] 4886 NM_014946.3(SPAST): c.1165A > G 6683 SPAST [′CTTTGGTCCACCTGGGAATGGGAAGRCAA (p.Thr389Ala) TGCTGGTAAGGGTTCTCTTCAA′] 4887 NM_000228.2(LAMB3): c.565 − 3914 LAMB3 [′TAAATCCATAAGGTTAAGTTGGACCYACA 2A > G GAGGGAAGGGAAAGAGAAGCGC′] 4888 NM_000492.3(CFTR): c.3368 − 1080 CFTR [′TCATTTACGTCTTTTGTGCATCTATDGGAG 2A > G AAGGAGAAGGAAGAGTTGGTA′] 4889 NM_006785.3(MALT1): c.1019 − 10892 MALT1 [′AACACCCCCTTTCTTTTTTTTTCAARGCGA 2A > G AGGACAAGGTTGCCCTTTTGA′] 4890 NM_004771.3(MMP20): c.611A > G 9313 MMP20 [′GGAGAAGGCCTGGGAGGAGATACACRTTT (p.His204Arg) CGACAATGCTGAGAAGTGGACT′] 4891 NM_000918.3(P4HB): c.1178A > G 5034 P4HB [′CACCCTAGAACTGCTTTCTTTTCAGRTGCC (p.Tyr393Cys) CCATGGTGTGGTCACTGCAAA′] 4892 NM_177405.2(CECR1): c.355A > G 51816 CECR1 [′TGCTCGCATCCCGCAGGCTCACCTGYTTCT (p.Thr119Ala) CCGGCGTGGAAGAAGTAAGGC′] 4893 NM_004990.3(MARS): c.1031A > G 4141 MARS [′TACCACATCATCCATGCTGACATCTRCCGC (p.Tyr344Cys) TGGTTTAACATTTCGTTTGAT′] 4894 NM_025132.3(WDR19): c.407 − 57728 WDR19 [′TCTGTATAAAAATAATCTCTTTTTCRGGAA 2A > G AACATACTAAGAGAATCACTT′] 4895 NM_000314.6(PTEN): c.139A > G 5728 PTEN [′TGCAGAAAGACTTGAAGGCGTATACRGGA (p.Arg47Gly) ACAATATTGATGATGTAGTAAG′] 4896 NM_000314.6(PTEN): c.182A > G 5728 PTEN [′GTTTTAAGGTTTTTGGATTCAAAGCDTAAA (p.His61Arg) AACCATTACAAGATATACAAT′] 4897 NM_000314.6(PTEN): c.254 − 2A > G 5728 PTEN [′TATTCTGAGGTTATCTTTTTACCACRGTTG CACAATATCCTTTTGAAGACC′] 4898 NM_000314.6(PTEN): c.320A > G 5728 PTEN [′GAACTTATCAAACCCTTTTGTGAAGRTCTT (p.Asp107Gly) GACCAATGGCTAAGTGAAGAT′] 4899 NM_000314.6(PTEN): c.512A > G 5728 PTEN [′CACCAGGGAGTAACTATTCCCAGTCRGAG (p.Gln171Arg) GCGCTATGTGTATTATTATAGC′] 4900 NM_000314.6(PTEN): c.802 − 2A > G 5728 PTEN [′TTTTCTTTTCTTTTTTTTTTTTTTTDGGACAA AATGTTTCACTTTTGGGTA′] 4901 NM_001103.3(ACTN2): c.1883A > 88 ACTN2 [′ATCCGCGATCAATCCCTGCAGGAGGRGCT G (p.Glu628Gly) GGCTCGCCAGCATGCTAACGAG′] 4902 NM_003159.2(CDKL5): c.462 − 6792 CDKL5 [′GCTTTTAATTGTGTTTGTTTTTTTCRGGGAG 2A > G TCATTTAATACTTCATGATT′] 4903 NM_003159.2(CDKL5): c.91A > G 6792 CDKL5 [′AGCCTATGGAGTTGTACTTAAATGCRGAC (p.Arg31Gly) ACAAGGCAAGTACATTATTTTT′] 4904 NM_003159.2(CDKL5): c.458A > G 6792 CDKL5 [′CACAATGATGTCCTAAAACTGTGTGRCTTT (p.Asp153Gly) GGTAAGTTAAAAAGAAATTAA′] 4905 NM_003159.2(CDKL5): c.2277 − 6792 CDKL5 [′ATATGATAAAAATGTCTTCTCATTTRGGAA 2A > G AAGTCCTGAAAATATTAGTCA′] 4906 NM_021098.2(CACNA1H): c.4645A > 8912 CACNA1H [′CATCGTCAGCTTCTTCGTGCTCAACRTGTT G (p.Met1549Val) CGTGGGCGTCGTGGTCGAGAA′] 4907 NM_003816.2(ADAM9): c.1396 − 8754 ADAM9 [′TGTTTAATTTGAATTCTATTTCACTRGTTCC 2A > G TTCCAGGAGGTACTTTATGC′] 4908 NM_212472.2(PRKAR1A): c.177 + 3A > 5573 PRKAR1A [′AATACTTTGAGAGGTTGGAGAAGGTRAAA G ATAAATGTGGGGAGATGATGAG′] 4909 NM_001204830.1(LIPT1): c.535A > −1 — [′CCGGACTACTGCCTATCACCATTGCRCTTT G (p.Thr179Ala) ATTATGTAGTACTGATGGGAC′] 4910 NM_001165963.1(SCN1A): c.5264A > −1 — [′AACCCTGGAAGCTCAGTTAAGGGAGRCTG G (p.Asp1755Gly) TGGGAACCCATCTGTTGGAATT′] 4911 NM_001165963.1(SCN1A): c.3880 − −1 — [′TAATTGTTATTATTTTTGTGTGTGCRGGTTT 2A > G CATTGGTCAGTTTAACAGCA′] 4912 NM_001165963.1(SCN1A): c.2537A > 6323 SCN1A [′TTTATTGTGACGCTTAGCCTGGTAGRACTT G (p.Glu846Gly) GGACTCGCCAATGTGGAAGGA′] 4913 NM_001165963.1(SCN1A): c.2353A > 6323 SCN1A [′GTCATTGGATAGTGCTCCATGGCCAHGAA G (p.Met785Val) AAGAGTATTTAAGACAATACAG′] 4914 NM_001165963.1(SCN1A): c.1662 + 6323 SCN1A [′AGAGGTACTCCTCCCCACACCAGGTRTGG 3A > G CACTGCTGAGTTTACTGATGCA′] 4915 NM_001165963.1(SCN1A): c.1076A > 6323 SCN1A [′TGTGTGAAAGCTGGTAGAAATCCCADTTA G (p.Asn359Ser) TGGCTACACAAGCTTTGATACC] 4916 NM_001165963.1(SCN1A): c.1048A > 6323 SCN1A [′TTGCAGCCAATGTCCAGAGGGATATRTGT G (p.Met350Val) GTGTGAAAGCTGGTAGAAATCC′] 4917 NM_001165963.1(SCN1A): c.1046A > 6323 SCN1A [′ACTTGCAGCCAATGTCCAGAGGGATRTAT G (p.Tyr349Cys) GTGTGTGAAAGCTGGTAGAAAT′] 4918 NM_001165963.1(SCN1A): c.433A > 6323 SCN1A [′CACTATTTTGACAAACTGTGTGTTTRTGAC G (p.Met145Val) AATGAGTAACCCTCCTGATTG′] 4919 NM_001165963.1(SCN1A): c.383 + 6323 SCN1A [′TAGCTATTAAGATTTTGGTACATTCRTATC 1A > G CTTTTTCAAGTGATTAATATT′] 4920 NM_001605.2(AARS): c.2251A > G 16 AARS [′TCGGCACCTGTGACAGCCACAATCCYCCG (p.Arg751Gly) GATACCCTTGGCAATGGCTTCT′] 4921 NM_000124.3(ERCC6): c.2830 − 2074 ERCC6 [′TATTCTCCATGCTCGCTCCCGGGCCYGCAA 2A > G CAGAGAGAGAGAGACCTCTCA′] 4922 NM_000124.3(ERCC6): c.2599 − 2074 ERCC6 [′ACTGGGAATGTGTATTTGCTTTGCAVACTC 26A > G CTATCCCCCACCTCCAAACAG′] 4923 NM_001110556.1(FLNA): c.1829 − 2316 FLNA [′ACTGAGGGGACTGGTGGCTGTTGTCRGGC 2A > G TTCTCGGTGGAAGGGCCATCGC′] 4924 NM_006129.4(BMP1): c.808A > G 649 BMP1 [′GGAGACCTATGACTTCGACAGCATCRTGC (p.Met270Val) ATTACGCTCGGAACACATTCTC′] 4925 NM_001987.4(ETV6): c.1252A > G 2120 ETV6 [′GGAGCCAGGACAAAGGCTTTTGTTCRGGT (p.Arg418Gly) AGCACTTCCTTTTTCTCCTTTC′] 4926 NM_014423.3(AFF4): c.760A > G 27125 AFF4 [′GTCCAATTCAATGTTACAGAAACCCRCTGC (p.Thr254Ala) CTATGTGCGGCCCATGGACGG′] 4927 NM_002055.4(GFAP): c.1085A > G 2670 GFAP [′AATGTCAAGCTGGCCCTGGACATCGRGAT (p.Glu362Gly) CGCCACCTACAGGAAGCTGCTA′] 4928 NM_002055.4(GFAP): c.256A > G 2670 GFAP [′TGACCGCTTTGCCAGCTACATCGAGRAGGT (p.Lys86Glu) TCGCTTCCTGGAACAGCAAAA′] 4929 NM_006017.2(PROM1): c.2077 − 8842 PROM1 [′ATTTGCTGTTCTTCACAGCTTTAGGRTATG 521A > G TATCCAATGTTTTCTTCAGGT′] 4930 NM_002755.3(MAP2K1): c.305A > 5604 MAP2K1 [′ACCTTTCTCCAGCTAATTCATCTGGRGATC G (p.Glu102Gly) AAACCCGCAATCCGGAACCAG′] 4931 NM_001288953.1(TTC7A): c.1715A > 57217 TTC7A [′CGTCCCCACAGCCTGATGTTCACCARGGTG G (p.Lys572Arg) AAGCTGGAGCAGGTGCTGAAA′] 4932 NM_016218.2(POLK): c.181A > G 51426 POLK [′TGAGCTCAAGAAAGAAAAGCAAGTCRACC (p.Asn6lAsp) AACGAATTGAAAATATGATGCA′] 4933 NM_016218.2(POLK): c.1385A > G 51426 POLK [′AGAACTGTTACCATTAAGTTGAAGARTGT (p.Asn462Ser) GAATTTTGAAGTAAAAACTCGT′] 4934 NM_016218.2(POLK): c.1477A > G 51426 POLK [′ATTTGCCATTGCTAAGGAATTGCTARAAAC (p.Lys493Glu) AGAAATTGATGCTGATTTTCC′] 4935 NM_001040431.2(COA3): c.215A > 28958 COA3 [′GGAAATCGAGTAGAAGGTGTAACCAYCTG G (p.Tyr72Cys) GGGAGGTAGGTTCAGGAAACCA′] 4936 NM_000891.2(KCNJ2): c.901A > G 3759 KCNJ2 [′TGAAATCGTGGTCATACTGGAAGGCRTGG (p.Met301Val) TGGAAGCCACTGCCATGACGAC′] 4937 NM_144499.2(GNAT1): c.386A > G 2779 GNAT1 [′GACATCATCCAGCGGCTGTGGAAGGRCTC (p.Asp129Gly) CGGTATCCAGGCCTGTTTTGAG′] 4938 NM_018965.3(TREM2): c.113A > G 54209 TREM2 [′CAGTCCCTGCAGGTGTCTTGCCCCTRTGAC (p.Tyr38Cys) TCCATGAAGCACTGGGGGAGG′] 4939 NM_194277.2(FRMD7): c.556A > G 90167 FRMD7 [′ACTGGACATAGCAAGGAAGCTGGATRTGT (p.Met186Val) ATGGCATCAGGCCTCACCCCGC′] 4940 NM_001003811.1(TEX11): c.511A > 56159 TEX11 [′TCACTCTCAACAGTAATCTTCTCCAYGGTC G (p.Met171Val) AAGTCAGCCTCAGGGGAGCTC′] 4941 NM_000921.4(PDE3A): c.1333A > G 5139 PDE3A [′AGTTTCTTCCACTTGGACCACCACCRCCTC (p.Thr445Ala) GGCCACAGGTCTACCCACCTT′] 4942 NM_000033.3(ABCD1): c.887A > G 215 ABCD1 [′GCCAACTCGGAGGAGATCGCCTTCTRTGG (p.Tyr296Cys) GGGCCATGAGGTGGGGCAGGTT′] 4943 NM_000169.2(GLA): c.137A > G −1 — [′AGGACGCCTACCATGGGCTGGCTGCDCTG (p.His46Arg) GGAGCGCTTCATGTGCAACCTT′] 4944 NM_000033.3(ABCD1): c.1992 − 215 ABCD1 [′CCCTGACCCTGTCCCTCTCCTGGCCRGGAA 2A > G ATACCACACACACTTGCTACA′] 4945 NM_000182.4(HADHA): c.919 − 3030 HADHA [′TTGCTCAATTCCAGTCTTTACCACCYAAAA 2A > G AACATATAAAGCACTTGCTCA′] 4946 NM_000159.3(GCDH): c.1213A > G 2639 GCDH [′CGAGTATCACGTGATCCGGCACGCCRTGA (p.Met405Val) ACCTGGAGGCCGTGAACACCTA′] 4947 NM_004006.2(DMD): c.1150 − 2A > G 1756 DMD [′ACAATTGTTAACTTCCTTCTTTGTCRGGGG TACATGATGGATTTGACAGCC′] 4948 NM_019109.4(ALG1): c.1188 − 56052 ALG1 [′CTCAGGCTCCCTTGGTTCTCTCTGCRGTTT 2A > G ACATGAGCTGGTGAAACATGA′] 4949 NM_004463.2(FGD1): c.2016 − 2245 FGD1 [′TCTTTCTTTTTTATTCCCCACCCCARGGACT 2A > G GAGGAGGAGAAGAAAGACTG′] 4950 NM_198525.2(KIF7): c.2981A > G 374654 KIF7 [′CTGCTGCTGGCTCTGGGCGCTGCCCYGCCG (p.Gln994Arg) CAGCTGCCCGCTCTTCTCGGA′] 4951 NM_020366.3(RPGRIP1): c.3749 − 57096 RPGRIP1 [′ATGCTGTTTTTTTCCCTTTCCCAACRGTTGT 2A > G TAGCCCTGAAGATCTGGCTA′] 4952 NM_003494.3(DYSF): c.3041A > G 8291 DYSF [′GTTCCCCCTCCCCCAGGCTGGGAGTRTAGC (p.Tyr1014Cys) ATCACCATCCCCCCGGAGCGG′] 4953 NM_003494.3(DYSF): c.3349 − 8291 DYSF [′CTGCCATAACCAGCTTCGTGTCTCCRGGGC 2A > G GGCGTGATGGATGACAAGAGT′] 4954 NM_004006.2(DMD): c.4675 − 2A > G 1756 DMD [′ATTACATTTCATTATAATTCTTTTCRGGTA ACAGAAAGAAAGCAACAGTTG′] 4955 NM_000169.2(GLA): c.620A > G −1 — [′GTGTACTCCTGTGAGTGGCCTCTTTRTATG (p.Tyr207Cys) TGGCCCTTTCAAAAGGTGAGA′] 4956 NM_022124.5(CDH23): c.146 − −1 — [′CTCTGCTCTCTCCCTTGGCTACTCCRGGTTC 2A > G TTCTGTGACCCAGTTGCTGG′] 4957 NM_000169.2(GLA): c.801 + 3A > G −1 — [′GGGGTTGGAATGACCCAGATATGGTRAAA ACTTGAGCCCTCCTTGTTCAAG′] 4958 NM_000019.3(ACAT1): c.473A > G 38 ACAT1 [′GCAGGTGGGATGGAGAGCATGTCCARTGT (p.Asn158Ser) TCCATATGTAATGAACAGAGGA′] 4959 NM_000553.4(WRN): c.561A > G 7486 WRN [′GTCTGGTTAAACACCTCTTAGGTAARCAGC (p.Lys187=) TCCTGAAAGACAAGTCTATCC′] 4960 NM_000050.4(ASS1): c.421 − 2A > G 445 ASS1 [′GGCTCTGACCCCTTGTCCTATGTCCDGGTC ATTGCTCCCTGGAGGATGCCT′] 4961 NM_020166.4(MCCC1): c.640 − 56922 MCCC1 [′TTCTGATCTAACAATCCTCATTCCCYAAGA 2A > G GAGAAAAGATGATTATGACTA′] 4962 NM_004006.2(DMD): c.10554 − 1756 DMD [′TCTTTTTTACTTTTTTGATGCCAATRGGAAT 2A > G CTGCAAGCAGAATATGACCG′] 4963 NM_017653.3(DYM): c.621 − 2A > G 54808 DYM [′ACAAGTTTGCTGGTGTATGGAAGACYATA CAAAAAGGAAAAAAAAATCAAA′] 4964 NM_001098398.1(COPA): c.728A > −1 — [′TCAGAATCAAAGGCATGGGAGGTTGRTAC G (p.Asp243Gly) CTGCCGGGGCCATTACAACAAT′] 4965 NM_005045.3(RELN): c.2288A > G 5649 RELN [′CGTCAGCTAATTACATCTTTCCTTGRCAGC (p.Asp763Gly) TCACAATCCAGGTGAGTGAAG′] 4966 NM_207111.3(RNF216): c.1616A > 54476 RNF216 [′TGCCATCTCTTTGATTTTCTGCTCAHAGAA G (p.Tyr539Cys) CTCCTGCTCTTGTTGCACAGC′] 4967 NM_001035.2(RYR2): c.568A > G 6262 RYR2 [′CATCTTAGTTAGCGTGTCCTCTGAARGGTA (p.Arg190Gly) CTTGGTAAGTGTGGAAAGTAG′] 4968 NM_001035.2(RYR2): c.11965A > G 6262 RYR2 [′AAATGCAACTGCTTTACCACCAGGTVATGT (p.Asn3989Asp) TGTTAATGGAACGATTGGCAA′] 4969 NM_001035.2(RYR2): c.12290A > G 6262 RYR2 [′GAACCTGCGAAGGACATCGGCTTCARCGT (p.Asn4097Ser) CGCCGTCCTTCTGACAAACCTC′] 4970 NM_001035.2(RYR2): c.12533A > G 6262 RYR2 [′AGACAGTTCATATTTGACGTGGTCARCGA (p.Asn4178Ser) AGGCGGAGAGAAAGAGAAGATG′] 4971 NM_000090.3(COL3A1): c.2338 − 1281 COL3A1 [′ACAGTGACATGGCTTCTCTTTTTCCRGGGT 2A > G GAAGGTGGTGCCCCCGGACTT′] 4972 NM_198056.2(SCN5A): c.4462A > G 6331 SCN5A [′CTTAGGGGGCCAGGACATCTTCATGRCAG (p.Thr1488Ala) AGGAGCAGAAGAAGTACTACAA′] 4973 NM_198056.2(SCN5A): c.4453A > G 6331 SCN5A [′CTCTTTGCACTTAGGGGGCCAGGACRTCTT (p.Ile1485Val) CATGACAGAGGAGCAGAAGAA′] 4974 NM_198056.2(SCN5A): c.2788 − 6331 SCN5A [′GGTGAGCCTGACCCATTATCTCGACRGGTC 2A > G CTGAATCTCTTCCTGGCCTTG′] 4975 NM_198056.2(SCN5A): c.1247A > G 6331 SCN5A [′GATGGTGGCTTGGTTTTGCTCCTCAYAGGC (p.Tyr416Cys) CATTGCGACCACGGCCAGGAT′] 4976 NM_004415.2(DSP): c.1141 − 2A > G 1832 DSP [′TTCACTGATCACTCTCATCCTTCACRGTTTT TTGAAGAGGCGCAGTCTACT′] 4977 NM_000238.3(KCNH2): c.2582A > 3757 KCNH2 [′TGGTCCAGCCTGGAGATCACCTTCANCCTG G (p.Asn861Ser) CGAGATGTGAGTTGGCTGCCC′] 4978 NM_000238.3(KCNH2): c.1913A > 3757 KCNH2 [′GTCTCTCCCAACACCAACTCAGAGARGAT G (p.Lys638Arg) CTTCTCCATCTGCGTCATGCTC′] 4979 NM_000238.3(KCNH2): c.1904A > 3757 KCNH2 [′TTCGGCAACGTCTCTCCCAACACCADCTCA G (p.Asn635Ser) GAGAAGATCTTCTCCATCTGC′] 4980 NM_000238.3(KCNH2): c.1900A > 3757 KCNH2 [′GGGCTTCGGCAACGTCTCTCCCAACRCCAA G (p.Thr634Ala) CTCAGAGAAGATCTTCTCCAT′] 4981 NM_000238.3(KCNH2): c.1129 − 3757 KCNH2 [′CCACACCTCCGCCTTCCCCGGGTGCRGGTC 2A > G CTGTCCCTGGGCGCCGACGTG′] 4982 NM_000238.3(KCNH2): c.296A > G 3757 KCNH2 [′GAGCGCAAAGTGGAAATCGCCTTCTVCCG (p.Tyr99Cys) GAAAGATGGTAGGAGCGGGCCG′] 4983 NM_000238.3(KCNH2): c.133A > G 3757 KCNH2 [′GGAGAACTGCGCCGTCATCTACTGCDACG (p.Asn45Asp) ACGGCTTCTGCGAGCTGTGCGG′] 4984 NM_001613.2(ACTA2): c.1A > G 59 ACTA2 [′AGAATCCTGTGAAGCAGCTCCAGCTRTGT (p.Met1Val) GTGAAGAAGAGGACAGCACTGC′] 4985 NM_000218.2(KCNQ1): c.605A > G 3784 KCNQ1 [′GCTCCCCCTCTCCTGCACTCCACAGRCCTC (p.Asp202Gly) ATCGTGGTCGTGGCCTCCATG′] 4986 NM_000218.2(KCNQ1): c.1033 − 3784 KCNQ1 [′GGGAGCCTCCTGTCCATTCCTTCCCVGGGG 2A > G ATTCTTGGCTCGGGGTTTGCC′] 4987 NM_000218.2(KCNQ1): c.1515 − 3784 KCNQ1 [′CAATCTCCTCTCCTCTCTCCACTGCRGGCT 2A > G GCGGGAACACCATCGGGCCAC′] 4988 NM_000218.2(KCNQ1): c.1787A > 3784 KCNQ1 [′ATCGGCGCCCGCCTGAACCGAGTAGRAGA G (p.Glu596Gly) CAAGGTAGGCTCACGCGCCGGC′] 4989 NM_000138.4(FBN1): c.7916A > G 2200 FBN1 [′TGCATGTGTCCCGCCGGCTTCCAGTRTGAA (p.Tyr2639Cys) CAGTTCAGTGGAGGATGCCAA′] 4990 NM_000138.4(FBN1): c.4337 − 2200 FBN1 [′TTTTGCTTTTTTCTCCCTCCCCCCARGATAT 2A > G TGATGAGTGCTCCCTTCCGA′] 4991 NM_000138.4(FBN1): c.3344A > G 2200 FBN1 [′GGTCATTTCCATTTTGCAGATATTGRTGAG (p.Asp1115Gly) TGTCAGAGAGATCCTCTCCTA′] 4992 NM_001943.3(DSG2): c.880A > G 1829 DSG2 [′AGTCAACGTAGAAGTTACGCGCATARAAG (p.Lys294Glu) TGTTCGATGCAGATGAAATAGG′] 4993 NM_001927.3(DES): c.1324A > G 1674 DES [′TGAGCAAAGGGGTTCTGAGGTCCATRCCA (p.Thr442Ala) AGAAGACGGTGATGATCAAGAC′] 4994 NM_004572.3(PKP2): c.1171 − 2A > G 5318 PKP2 [′AGAAATATGCATCTGCTTCTTCCCCRGGTT AACCAGCTTCGTGGCATCCTC′] 4995 NM_001256850.1(TTN): c.45629 − −1 — [′GATATGAATATTTCACTCTTTTCTCRGGTC 2A > G CTCCCTCACCACCCCTTGACC′] 4996 NM_013254.3(TBK1): c.1201A > G 29110 TBK1 [′ACTTCATATTTCAGTTTCCCTCCCTRAAGT (p.Lys401Glu) ACATCCACGTTATGATTTAGA′] 4997 NM_030973.3(MED25): c.116A > G 81857 MED25 [′TACTTCGAGGGGCTCCGCAAGCACTRCCTG (p.Tyr39Cys) CTCCCGGCCATCGAGTGAGTG′] 4998 NM_145207.2(SPATA5): c.1883A > 166378 SPATA5 [′TCTATTTTTCAGGTATCCTGGTCAGRTATA G (p.Asp628Gly) GGAGGACTGGAAAGTATCAAA′] 4999 NM_000016.5(ACADM): c.329A > 34 ACADM [′ACTTTTGATGCTTGTTTAATTAGTGRAGAA G (p.Glu110Gly) TTGGCTTATGGATGTACAGGG′] 5000 NM_000016.5(ACADM): c.797A > 34 ACADM [′CCTAAAGAAAATGTTTTAATTGGTGRCGG G (p.Asp266Gly) AGCTGGTTTCAAAGTTGCAATG′] 5001 NM_000532.4(PCCB): c.655 − 2A > G 5096 PCCB [′GACTGTTCTGGAAATCTTTTATTTCRGGAC ACCTCCTACCTGTTCATCACT′] 5002 NM_004453.3(ETFDH): c.929A > G 2110 ETFDH [′CATACCTATGGAGGATCTTTCCTCTRTCAT (p.Tyr310Cys) TTGAATGAAGGTGAACCCCTA′] 5003 NM_000255.3(MUT): c.1885A > G 4594 MUT [′AAAAATGGGACAAGATGGCCATGACRGAG (p.Arg629Gly) GAGCAAAAGTTATTGCTACAGG′] 5004 NM_000255.3(MUT): c.329A > G 4594 MUT [′TTTAGGCCCTGGACCATCCGCCAGTRTGCT (p.Tyr110Cys) GGTTTTAGTACTGTGGAAGAA′] 5005 NM_000017.3(ACADS): c.1108A > 35 ACADS [′TCAGGCCATCCAGATCCTGGGCGGCRTGG G (p.Met370Val) GCTACGTGACAGAGATGCCGGC′] 5006 NM_174917.4(ACSF3): c.1A > G 197322 ACSF3 [′TCCAGCTCGGCCGCCTGTCAGTGCARTGCT (p.Met1Val) GCCCCATGTGGTGCTCACCTT′] 5007 NM_000531.5(OTC): c.919A > G 5009 OTC [′GACATTTTTACACTGCTTGCCCAGARAGCC (p.Lys307Glu) AGAAGAAGTGGATGATGAAGT′] 5008 NM_000030.2(AGXT): c.248A > G 189 AGXT [′ACACTGGTCATCTCTGGCTCGGGACRCTGT (p.His83Arg) GCCCTGGAGGCCGCCCTGGTC′] 5009 NM_000030.2(AGXT): c.424 − 2A > G 189 AGXT [′CACCCACAGCCGTCCCTGCTTCCTCRGGGC (p.Gly_142Gln145del) CTGGCCCAGCACAAGCCAGTG′] 5010 NM_000030.2(AGXT): c.596 − 2A > G 189 AGXT [′CGTCCCGAGCAAACCACCCATCTACRGGC ATCGACATCCTGTACTCGGGCT′] 5011 NM_000030.2(AGXT): c.777 − 2A > G 189 AGXT [′TGGACCAAGCCCCCTCGTGTCTTCCRGGTA CCATCACACAATCCCCGTCAT′] 5012 NM_012203.1(GRHPR): c.84 − 2A > G 9380 GRHPR [′CTCCTGAGGGCCTCCCTTTCCCCGCRGCTG TGAGGTGGAGCAGTGGGACTC′] 5013 NM_012203.1(GRHPR): c.934A > G 9380 GRHPR [′CACCATGTCCTTGTTGGCAGCTAACRACTT (p.Asn312Asp) GCTGGCTGGCCTGAGAGGGGA′] 5014 NM_203290.2(POLR1C): c.221A > 9533 POLR1C [′GTGGGAATTGACGCAGCCATTGCCARTGC G (p.Asn74Ser) TTTTCGACGAATTCTGCTAGCT′] 5015 NM_006516.2(SLC2A1): c.848A > G 6513 SLC2A1 [′GCTGTGGTGCTGCAGCTGTCCCAGCRGCTG (p.Gln283Arg) TCTGGCATCAACGCTGTGAGT′] 5016 NM_006516.2(SLC2A1): c.19 − 6513 SLC2A1 [′ATAACAGTGTGGTTTGTTTCTCCGCRGAAG 2A > G CTGACGGGTCGCCTCATGCTG′] 5017 NM_021007.2(SCN2A): c.387 − 6326 SCN2A [′ACTTTGTCTTCCTTGACGATATTCTRCTTTA 2A > G TTCAATATGCTCATTATGTG′] 5018 NM_021007.2(SCN2A): c.851A > G 6326 SCN2A [′AATAAATGTTTGCAATGGCCTCCAGRTAAT (p.Asp284Gly) TCTTCCTTTGAAATAAATATC′] 5019 NM_021007.2(SCN2A): c.4036A > G 6326 SCN2A [′CATGAATGTACTTCTGGTTTGTCTGRTCTTT (p.Ile1346Val) TGGCTAATATTCAGTATCAT′] 5020 NM_001165963.1(SCN1A): c.4476 + −1 — [′TAATTTCAACCAGCAGAAAAAGAAGDTAA 1A > G GTATTTCTAATATTTTCTCTCC′] 5021 NM_001165963.1(SCN1A): c.1277A > 6323 SCN1A [′ATCCTGGCTGTGGTGGCCATGGCCTRCGAG G (p.Tyr426Cys) GAACAGAATCAGGCCACCTTG′] 5022 NM_000391.3(TPP1): c.833A > G 1200 TPP1 [′GGGATTGAGGCCAGTCTAGATGTGCRGTA (p.Gln278Arg) CCTGATGAGTGCTGGTGCCAAC′] 5023 NM_017882.2(CLN6): c.767A > G 54982 CLN6 [′GGAGAAGAGGAAGAGGCCGTTGCTGYCCA (p.Asp256Gly) GGAAGAGGCGCTTGCGCTTCTG′] 5024 NM_002693.2(POLG): c.3470A > G 5428 POLG [′GGGCCCCGCATACCTGGTCAAGAGGYTGG (p.Asn1157Ser) TGATCTGCAAGGCCAGGGCAGC′] 5025 NM_002693.2(POLG): c.2840A > G 5428 POLG [′GTGGGCATCAGCCGTGAGCATGCCARAAT (p.Lys947Arg) CTTCAACTACGGCCGCATCTAT′] 5026 NM_002693.2(POLG): c.2636A > G 5428 POLG [′CACAAGGGTGTAGCCAGGTGGGGCCYGCA (p.Gln879Arg) CCATGGCTTTCAACTCACTGCC′] 5027 NM_000833.4(GRIN2A): c.2449A > 2903 GRIN2A [′GAGCAGCCAGCTGGACATTGACAACRTGG G (p.Met817Val) CGGGCGTATTCTACATGCTGGC′] 5028 NM_000833.4(GRIN2A): c.1930A > 2903 GRIN2A [′CTTCTTCGCTGTCATATTCCTGGCTRGCTA G (p.Ser644Gly) CACAGCCAATCTGGCTGCCTT′] 5029 NM_000156.5(GAMT): c.1A > G 2593 GAMT [′GTCGGGTCGCCGTCCAGCCTGCAGCRTGA (p.Met1Val) GCGCCCCCAGCGCGACCCCCAT′] 5030 NM_172107.2(KCNQ2): c.848A > G 3785 KCNQ2 [′CTGACCACCATTGGCTACGGGGACARGTA (p.Lys283Arg) CCCCCAGACCTGGAACGGCAGG′] 5031 NM_172107.2(KCNQ2): c.611A > G 3785 KCNQ2 [′GCGCTCCGGAGCCTGCGCTTCCTGCRGATT (p.Gln204Arg) CTGCGGATGATCCGCATGGAC′] 5032 NM_172107.2(KCNQ2): c.297 − 3785 KCNQ2 [′CTGAGAGCGCGCGTTCCCTGCCCCCRGGTT 2A > G CCTCCTGGTTTTCTCCTGCCT′] 5033 NM_001105243.1(PCDH19): c.1019A > 57526 PCDH19 [′GTCACCGTCAGCGTGCTGGACACCARTGA G (p.Asn340Ser) CAATCCGCCGGTCATCAACCTG′] 5034 NM_001105243.1(PCDH19): c.695A > 57526 PCDH19 [′CTTAGTATCAAGGTGACCGACTCCADTGA G (p.Asn232Ser) CAACAACCCGGTGTTTAGCGAG′] 5035 NM_052859.3(RFT1): c.454A > G 91869 RFT1 [′TTCACAGCAGAAGGTGCACTGACCTBGAG (p.Lys152Glu) CTTCACAAACATATGTGCTTGT′] 5036 NM_052859.3(RFT1): c.1222A > G 91869 RFT1 [′TCTCCTCCCCAGGTACAATTTTGTGRTGCT (p.Met408Val) GGCCCTGTCCTCCTCATTCCT′] 5037 NM_020533.2(MCOLN1): c.1406A > 57192 MCOLN1 [′TCTGAGTGCCTGTTCTCGCTCATCARTGGG G (p.Asn469Ser) GACGACATGTTTGTGACGTTC′] 5038 NM_004456.4(EZH2): c.458A > G 2146 EZH2 [′TTCATTGAAGAACTAATAAAAAATTRTGAT (p.Tyr153Cys) GGGAAAGTACACGGGGATAGA′] 5039 NM_005045.3(RELN): c.2168A > G 5649 RELN [′ACCACGGATAGAGTAAAAGTTATGGYAAG (p.Tyr723Cys) AGGAGAGCCTGGAACTGCCAAA] 5040 NM_006772.2(SYNGAP1): c.388 − 8831 SYNGAP1 [′CCCACCCCATCCCCATTTCCCCCCCRGCAA 2A > G GGCTTCCTGAGCCGACGGCTA′] 5041 NM_001080508.2(TBX18): c.487A > 9096 TBX18 [′GATAGGCACTGAGATGATCATCACCRAGG G (p.Lys163Glu) CCGGCAGGTAATGGGCAAGCTG′] 5042 NM_004964.2(HDAC1): c.461A > G 3065 HDAC1 [′GAGGCATCTGGCTTCTGTTACGTCARTGAT (p.Asn154Ser) ATCGTCTTGGCCATCCTGGAA′] 5043 NM_014946.3(SPAST): c.1168A > G 6683 SPAST [′TGGTCCACCTGGGAATGGGAAGACARTGC (p.Met390Val) TGGTAAGGGTTCTCTTCAAATT′] 5044 NM_000314.6(PTEN): c.71A > G 5728 PTEN [′TATCAAGAGGATGGATTCGACTTAGRCTTG (p.Asp24Gly) ACCTGTATCCATTTCTGCGGC′] 5045 NM_004380.2(CREBBP): c.4409A > 1387 CREBBP [′GGGTCCTGCAGGTATGTGACAGGGCRCAT G (p.His1470Arg) CTGGGCCTGTCCTCCAAGTGAA′] 5046 NM_018206.4(VPS35): c.1463A > G 55737 VPS35 [′GATCCAGAAGATTTTGCTGATGAGCRGAG (p.Gln488Arg) CCTTGTGGGCCGCTTCATTCAT′] 5047 NM_152296.4(ATP1A3): c.971A > G 478 ATP1A3 [′GGCATCATCGTGGCCAATGTCCCAGRGGG (p.Glu324Gly) TCTGCTGGCCACTGTCACTGTA′] 5048 NM_172107.2(KCNQ2): c.710A > G 3785 KCNQ2 [′TCTCAGGAGCTGGTCACTGCCTGGTRCATC (p.Tyr237Cys) GGCTTCCTTTGTCTCATCCTG′] 5049 NM_001017980.3(VMA21): c.163 + 203547 VMA21 [′ACTAAATCTTACATATTTGAAGGTARTCTT 4A > G AGACCCATTAAAACAAGATGT′] 5050 NM_000489.4(ATRX): c.740A > G 546 ATRX [′TTCTGCAAGAAATGCATTCTACGCARCCTT (p.Asn247Ser) GGTCGAAAGGAGTTGTCCACA′] 5051 NM_021147.4(CCNO): c.716A > G 10309 CCNO [′CCCACCATTAGCTTCTTCCTGGAGCRTTTC (p.His239Arg) ACGCACGCTCGCGTGGAGGCG′] 5052 NM_031924.4(RSPH3): c.631 − 83861 RSPH3 [′CTCTAGAGAATCAGGCCGTCCGAGCYAAC 2A > G AGTGATAGAAAATACTTCTAGA′] 5053 NM_000069.2(CACNA1S): c.3526 − 779 CACNA1S [′TCGCTTTCCCATCCTTTTCCTTCCCRGGGCT 2A > G ACTTTGGAGACCCCTGGAAT′] 5054 NM_000533.4(PLP1): c.1A > G 5354 PLP1 [′CAATTGGAGTCAGAGTCCCAAAGACRTGG (p.Met1Val) GTAAGTTTCAAAAACTTTAGCA′] 5055 NM_207352.3(CYP4V2): c.1396A > 285440 CYP4V2 [′CGTGCCCTTCTCTGCTGGCCCCAGGRACTG G (p.Asn466Asp) TATAGGTTTGTATCCATCTGA′] 5056 NM_033409.3(SLC52A3): c.403A > 113278 SLC52A3 [′ACAAAGAAGGTGGTGAGGTAGTAGGYGG G (p.Thr135Ala) GCAGCCGGCTCATGAACGGCAGG′] 5057 NM_033409.3(SLC52A3): c.62A > G 113278 SLC52A3 [′CAGGGGCAGCTCTACCCAGAGCCCAYTGA (p.Asn21Ser) TGGTCACCCAGGAGCCCATTCC′] 5058 NM_017662.4(TRPM6): c.3173A > 140803 TRPM6 [′CAAGCTGTCTACCTCTTCGTGCAATRTATC G (p.Tyr1058Cys) ATCATGGTGAACCTGTTGATT′] 5059 NM_006642.3(SDCCAG8): c.221 − 10806 SDCCAG8 [′AATAAACCCTCTGCTTTTGCTCTATRGTTA 2A > G ATCAGCTCAAAGATTTGTTGC′] 5060 NM_022455.4(NSD1): c.5990A > G 64324 NSD1 [′CAAGAACATGATATCACTAATTTCTRTATG (p.Tyr1997Cys) CTCACCCTAGACAAAGTAAGT′] 5061 NM_000352.4(ABCC8): c.563A > G 6833 ABCC8 [′GGGATGCTGCTCCTCGTGGAGGTCARTGTC (p.Asn188Ser) ATCAGGGTGAGGGTAAGCAGG] 5062 NM_000275.2(OCA2): c.1427A > G 4948 OCA2 [′GATGGCAGTGGCAGCTCCTCCAATGYTTGT (p.Asn476Ser) GAAGATCACTTCTGCAATCAG′] 5063 NM_001848.2(COL6A1): c.1003 − 1291 COL6A1 [′ATTTTCTAGTTTTCTTCCTCTTTCCRGGGGG 2A > G AGATGGGGTACCCAGGCCTG′] 5064 NM_003560.2(PLA2G6): c.1349 − 8398 PLA2G6 [′CAGCATGCCCTGCTCTGTGCCTCACRGAAC 2A > G TACAGGATCTCATGCACATCT′] 5065 NM_178151.2(DCX): c.280A > G 1641 DCX [′TGACCTGACGCGATCTCTGTCTGACRACAT (p.Asn94Asp) CAACCTGCCTCAGGGAGTGCG′] 5066 NM_000425.4(L1CAM): c.2351A > 3897 L1CAM [′TCCAACACGTCCACCTTCGTGCCCTRTGAG G (p.Tyr784Cys) ATCAAAGTCCAGGCCGTCAAC′] 5067 NM_000052.6(ATP7A): c.2173 − 538 ATP7A [′TCAATGATTATCATTCCTATATTGCRGTTTT 2A > G TCGGAGGCTGGTACTTCTAC′] 5068 NM_020247.4(ADCK3): c.1286A > 56997 ADCK3 [′CTGCTGAAGGGCCACCCCTTCTTCTRTGTG G (p.Tyr429Cys) CCTGAGATTGTGGATGAGCTC′] 5069 NM_000143.3(FH): c.554A > G 2271 FH [′AAACAGCAAAGCTCACATACTGACCYGGC (p.Gln185Arg) TTTTATTAACATGATCGTTGGG′] 5070 NM_033109.4(PNPT1): c.1453A > G 87178 PNPT1 [′AAACTTCCGCCACATGCAGATGCCAKAGA (p.Met485Val) AGATGACCCTATAGAAAGAAAA′] 5071 NM_005888.3(SLC25A3): c.158 − 5250 SLC25A3 [′TACTTACTTGATTTTTTTTTTTCCARTCAAA 9A > G CAGAGCAGTATAGCTGTGAC′] 5072 NM_181426.1(CCDC39): c.610 − 339829 CCDC39 [′TTGTGCTGCTTTATCCAATTCTAACYGTCA 2A > G AACAGAGAGCAAAGAACATTT′] 5073 NM_153704.5(TMEM67): c.725A > 91147 TMEM67 [′ATTGTTCTGTTGTAGGTATATGCCARTCTA G (p.Asn242Ser) ACATCTTGTCAAGCTCTTGGA′] 5074 NM_000166.5(GJB1): c.305A > G 2705 GJB1 [′CACGTGGCTCACCAGCAACACATAGRGAA (p.Glu102Gly) GAAAATGCTACGGCTTGAGGGC′] 5075 NM_019098.4(CNGB3): c.1193A > 54714 CNGB3 [′AATTAAAGTTCGAACTGCCCAATAAYAAC G (p.Tyr398Cys) ATCTCAGATACCTGTGAAAACA′] 5076 NM_000070.2(CAPN3): c.1194 − 825 CAPN3 [′CCATATGGCTCTCTCTCTTCTTCCARCCTCT 9A > G CAGGATGTCCTATGAGGATT′] 5077 NM_002860.3(ALDH18A1): c.2345A > 5832 ALDH18A1 [′AGGAATAGGGAGGTTCTCATGAAGABATT G (p.Tyr782Cys) TTAAACTTCCATGCTCTGAGAA′] 5078 NM_001414.3(EIF2B1): c.824A > G 1967 EIF2B1 [′CAGAGTGATTAAGGAAGGGGCAGTGYAGT (p.Tyr275Cys) CGACCCACGGATGCTCCTCTTT′] 5079 NM_182896.2(ARL13B): c.461A > G 200894 ARL13B [′TGTCTATCTCTGGAAAAATTGGTCARTGAG (p.Asn154Ser) CACAAGTGCCTGTGTCAGATA′] 5080 NM_001134831.1(AHI1): c.1152 − 54806 AHI1 [′TAGTAAGATGAAACAGGCCGTCCACYGTA 2A > G CAAAAAAAGATACTTCCATTAA′] 5081 NM_153704.5(TMEM67): c.978 + 3A > 91147 TMEM67 [′TCAGTTTTAAAGGAGAAAACCAGGTRAAA G GTGTCTAATATCATTAGAGGAT′] 5082 NM_015681.3(B9D1): c.95A > G 27077 B9D1 [′CCAGTCCTGGCCGTACACAAAGCAGYACT (p.Tyr32Cys) TGCAGTAGAGGTCATCATACTC′] 5083 NM_014049.4(ACAD9): c.1A > G 28976 ACAD9 [′CTGAGGCTGGGGAACATCGGGCAGCWTGA (p.Met1Val) GCGGCTGCGGGCTCTTCCTGCG′]

TABLE 8 xCas9v3 Mutations (K294R/Q1256K Series) xCas9 3.0 xCas9 3.1 xCas9 3.2 xCas9 3.3 xCas9 3.4 N175T P230F D257N S267G S267G S267G K294R K294R K294R K294R K294R T466A E480K E480K E480K E480K E480K E543D E543D E543D E543D E543D A711E E1207G I1063V E1219V E1219V E1219V E1219V E1219V Q1256K Q1256K Q1256K Q1256K Q1256K

TABLE 9 xCas9v3 Mutations (A262T/S409I Series) xCas9 3.5 xCas9 3.6 xCas9 3.7 xCas9 3.8 xCas9 3.9 xCas9 3.10 xCas9 3.11 xCas9 3.12 E108G E108G E108G E108G S217A A262T A262T A262T A262T A262T A262T A262T A262T R324L S409I S409I S409I S409I S409I S409I S409I S409I E480K E480K E480K E480K E480K E480K E480K E480K E543D E543D E543D E543D E543D E543D E543D E543D D605D D605D K673E K673E M694I M694I M694I M694I M694I M694I M694I M694I E1219V E1219V E1219V E1219V E1219V E1219V E1219V E1219V H1264Y L1365I L1365I L1365I L1365I

TABLE 10 PAM Depletion Scores (xCas9v3.0-3.6 Mutations) 22-4 22-10 22-11 22-14 22-21 22-65 22-66 xCas9 3.0 xCas9 3.1 xCas9 3.2 xCas9 3.3 xCas9 3.4 xCas9 3.5 xCas9 3.6 AAA 0.61 0.69 1.38 0.90 0.98 1.29 1.47 AAC 0.67 0.77 0.78 0.71 0.93 0.80 1.55 AAG 13.88 15.70 32.70 28.75 22.79 48.60 92.02 AAT 0.52 0.59 0.87 0.63 0.76 0.87 0.84 ACA 0.52 0.52 0.48 0.51 0.57 0.59 0.72 ACC 0.54 0.50 0.48 0.48 0.47 0.46 0.55 ACG 10.15 30.07 41.38 108.50 32.19 25.52 158.44 ACT 0.46 0.47 0.39 0.41 0.44 0.40 0.50 AGA 13.22 28.30 34.42 57.31 38.26 31.93 170.85 AGC 7.55 5.84 11.17 8.69 7.63 13.13 5.54 AGG 12.23 188.82 37.97 182.49 194.93 82.99 355.32 AGT 13.19 9.75 17.03 16.09 10.90 19.84 22.47 ATA 0.58 0.56 0.51 0.52 0.51 0.53 0.49 ATC 0.52 0.50 0.45 0.45 0.47 0.42 0.38 ATG 11.38 14.85 23.55 40.11 20.48 30.27 108.77 ATT 0.48 0.46 0.43 0.42 0.43 0.41 0.39 CAA 1.55 2.00 6.63 5.06 3.42 6.29 4.66 CAC 0.57 0.65 0.99 0.75 0.86 0.93 3.20 CAG 15.05 11.83 26.90 26.86 22.15 32.07 137.31 CAT 4.41 6.06 17.67 7.55 6.30 8.13 7.87 CCA 0.48 0.46 0.41 0.43 0.42 0.40 0.39 CCC 0.53 0.51 0.46 0.45 0.45 0.43 0.44 CCG 3.35 2.67 6.87 6.98 4.69 8.56 5.44 CCT 0.53 0.50 0.45 0.49 0.46 0.43 0.38 CGA 9.23 49.45 43.50 106.20 62.04 27.17 116.32 CGC 13.79 8.59 17.62 15.06 12.16 21.40 11.61 CGG 16.85 223.84 46.41 346.14 96.28 90.82 252.73 CGT 12.66 23.87 36.06 42.02 20.18 27.33 179.69 CTA 0.54 0.50 0.45 0.47 0.47 0.45 0.49 CTC 0.51 0.48 0.43 0.44 0.45 0.42 0.38 CTG 5.39 3.40 6.92 8.28 5.95 11.34 4.20 CTT 0.52 0.52 0.46 0.46 0.44 0.44 0.82 GAA 10.98 7.67 14.45 11.25 9.75 11.86 6.86 GAC 0.88 1.35 4.85 2.92 2.40 5.25 4.15 GAG 11.85 55.33 39.91 118.83 109.84 85.83 390.44 GAT 7.69 5.68 10.55 9.22 6.41 11.03 9.53 GCA 0.49 0.49 0.47 0.52 0.53 0.59 0.61 GCC 0.53 0.52 0.50 0.50 0.49 0.46 0.52 GCG 13.68 13.44 22.56 46.72 32.75 35.86 97.69 GCT 0.51 0.50 0.45 0.48 0.46 0.45 0.61 GGA 9.74 82.32 39.45 172.39 102.30 85.53 77.46 GGC 12.21 10.04 17.84 15.18 14.04 27.07 17.46 GGG 18.90 381.76 70.36 281.12 164.21 111.87 718.40 GGT 10.87 22.11 32.25 88.31 40.66 40.16 257.92 GTA 0.56 0.59 0.77 0.72 0.76 1.03 1.55 GTC 0.52 0.51 0.46 0.52 0.49 0.45 0.58 GTG 11.43 16.42 28.54 72.25 33.31 47.65 382.48 GTT 0.48 0.45 0.41 0.43 0.43 0.42 0.76 TAA 0.58 0.67 1.59 0.99 1.04 1.69 1.53 TAC 0.56 0.54 0.51 0.51 0.52 0.50 0.44 TAG 11.34 10.19 15.99 22.04 15.24 27.89 35.59 TAT 0.56 0.64 1.26 0.85 0.89 1.21 2.60 TCA 0.50 0.48 0.43 0.45 0.46 0.45 0.44 TCC 0.50 0.48 0.43 0.46 0.44 0.42 0.47 TCG 10.38 8.24 14.95 15.64 9.96 15.57 34.80 TCT 0.52 0.50 0.45 0.44 0.46 0.41 0.46 TGA 9.68 61.64 44.45 188.12 99.42 46.89 186.41 TGC 13.64 9.07 15.11 11.69 11.22 15.01 8.15 TGG 12.90 223.88 63.67 259.65 115.56 80.51 210.65 TGT 11.53 18.90 31.99 36.30 18.72 21.90 122.87 TTA 0.61 0.57 0.52 0.54 0.51 0.52 0.30 TTC 0.53 0.50 0.44 0.45 0.44 0.43 0.27 TTG 13.57 6.61 10.15 13.27 7.38 14.26 12.24 TTT 0.53 0.49 0.44 0.45 0.48 0.44 0.28 PAM Depletion Scores (xCas9v3.7-3.12 Mutations) 22-68 22-75 22-78 22-82 22-87 22-90 xCas9 3.7 xCas9 3.8 xCas9 3.9 xCas9 3.10 xCas9 3.11 xCas9 3.12 AAA 0.92 1.00 4.30 0.63 0.48 1.67 AAC 8.72 3.59 1.55 1.59 0.94 1.34 AAG 23.44 20.16 16.46 10.63 26.46 22.44 AAT 1.03 0.89 1.22 1.07 0.76 0.96 ACA 0.80 0.92 0.52 0.68 1.09 0.83 ACC 0.42 1.37 0.32 1.03 0.81 0.53 ACG 11.20 6.65 21.80 1.38 1.23 0.88 ACT 0.27 0.38 0.24 0.88 0.54 0.66 AGA 11.63 11.95 14.60 0.89 1.42 1.78 AGC 1.03 11.25 8.05 1.54 1.77 2.39 AGG 8.05 11.61 21.28 1.37 33.28 113.98 AGT 11.10 6.85 12.73 0.39 2.70 0.91 ATA 0.73 0.79 0.58 1.04 0.70 0.94 ATC 0.43 0.47 0.28 0.63 0.86 0.87 ATG 15.46 13.03 4.76 2.21 2.39 1.73 ATT 0.62 0.30 0.53 0.59 0.91 0.74 CAA 8.96 6.08 1.97 1.46 0.53 0.67 CAC 1.65 1.31 0.81 0.60 0.75 0.73 CAG 3.46 1.99 1.44 23.50 9.51 13.76 CAT 20.52 44.57 18.70 16.29 15.96 0.36 CCA 0.40 0.31 0.64 0.87 0.55 0.64 CCC 0.35 0.54 0.55 1.12 0.56 0.69 CCG 4.35 5.28 5.57 1.37 0.94 0.83 CCT 0.40 1.04 0.36 1.22 0.67 0.59 CGA 4.47 7.60 5.26 1.35 1.36 2.02 CGC 13.35 10.98 5.45 4.03 5.37 3.66 CGG 6.06 5.52 25.23 31.85 19.44 34.65 CGT 17.13 7.41 17.31 0.63 1.44 0.66 CTA 0.76 0.50 0.50 1.97 1.45 1.05 CTC 0.40 0.26 0.65 0.58 0.45 0.81 CTG 5.64 9.49 6.82 1.35 1.41 2.11 CTT 0.50 0.63 0.68 0.54 0.73 0.88 GAA 9.81 6.07 4.68 2.07 0.87 1.09 GAC 3.91 3.95 2.82 0.44 0.86 0.56 GAG 23.26 21.32 9.88 16.93 18.42 40.73 GAT 5.06 9.85 1.07 0.75 1.06 0.63 GCA 0.56 0.81 0.79 1.46 0.44 0.89 GCC 0.35 0.52 0.71 0.65 3.67 0.73 GCG 16.11 12.53 9.43 1.59 1.98 2.22 GCT 0.42 0.88 0.27 0.66 1.14 0.76 GGA 3.01 18.40 33.07 27.28 16.20 46.82 GGC 6.03 3.83 11.69 8.03 22.56 3.92 GGG 12.16 10.26 15.08 17.30 25.85 128.03 GGT 11.25 13.77 2.94 12.71 16.11 16.22 GTA 1.38 1.08 1.24 0.75 3.52 1.09 GTC 0.96 0.31 0.50 0.93 0.72 0.81 GTG 6.68 17.93 26.86 9.93 9.61 2.72 GTT 0.45 0.33 0.99 0.65 1.61 1.01 TAA 3.04 2.16 1.84 1.21 1.08 0.68 TAC 0.61 0.52 0.68 0.95 0.68 0.86 TAG 4.76 15.71 1.10 8.15 8.25 8.87 TAT 1.62 2.10 1.01 0.73 0.74 0.66 TCA 0.41 0.66 0.58 0.46 0.77 0.68 TCC 0.54 0.29 0.38 1.36 0.86 0.82 TCG 1.41 4.20 9.11 0.84 0.46 0.69 TCT 0.47 0.44 0.38 0.60 1.00 0.94 TGA 22.42 5.39 15.72 1.78 3.13 3.69 TGC 6.78 6.81 11.35 2.41 6.55 4.08 TGG 20.36 11.36 11.56 9.97 21.64 19.87 TGT 10.32 8.82 21.17 0.94 0.79 0.73 TTA 0.65 0.29 0.77 0.94 1.35 0.75 TTC 0.62 1.07 0.55 0.86 1.07 0.58 TTG 8.41 6.07 9.07 0.67 0.56 0.72 TTT 0.43 0.56 0.44 0.68 0.60 0.75

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All publications, patents, patent applications, publication, and database entries (e.g., sequence database entries) mentioned herein, e.g., in the Background, Summary, Detailed Description, Examples, and/or References sections, are hereby incorporated by reference in their entirety as if each individual publication, patent, patent application, publication, and database entry was specifically and individually incorporated herein by reference. In case of conflict, the present application, including any definitions herein, will control.

EQUIVALENTS AND SCOPE

Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents of the embodiments described herein. The scope of the present disclosure is not intended to be limited to the above description, but rather is as set forth in the appended claims.

Articles such as “a,” “an,” and “the” may mean one or more than one unless indicated to the contrary or otherwise evident from the context. Claims or descriptions that include “or” between two or more members of a group are considered satisfied if one, more than one, or all of the group members are present, unless indicated to the contrary or otherwise evident from the context. The disclosure of a group that includes “or” between two or more group members provides embodiments in which exactly one member of the group is present, embodiments in which more than one members of the group are present, and embodiments in which all of the group members are present. For purposes of brevity those embodiments have not been individually spelled out herein, but it will be understood that each of these embodiments is provided herein and may be specifically claimed or disclaimed.

It is to be understood that the invention encompasses all variations, combinations, and permutations in which one or more limitation, element, clause, or descriptive term, from one or more of the claims or from one or more relevant portion of the description, is introduced into another claim. For example, a claim that is dependent on another claim can be modified to include one or more of the limitations found in any other claim that is dependent on the same base claim. Furthermore, where the claims recite a composition, it is to be understood that methods of making or using the composition according to any of the methods of making or using disclosed herein or according to methods known in the art, if any, are included, unless otherwise indicated or unless it would be evident to one of ordinary skill in the art that a contradiction or inconsistency would arise.

Where elements are presented as lists, e.g., in Markush group format, it is to be understood that every possible subgroup of the elements is also disclosed, and that any element or subgroup of elements can be removed from the group. It is also noted that the term “comprising” is intended to be open and permits the inclusion of additional elements or steps. It should be understood that, in general, where an embodiment, product, or method is referred to as comprising particular elements, features, or steps, embodiments, products, or methods that consist, or consist essentially of, such elements, features, or steps, are provided as well. For purposes of brevity those embodiments have not been individually spelled out herein, but it will be understood that each of these embodiments is provided herein and may be specifically claimed or disclaimed.

Where ranges are given, endpoints are included. Furthermore, it is to be understood that unless otherwise indicated or otherwise evident from the context and/or the understanding of one of ordinary skill in the art, values that are expressed as ranges can assume any specific value within the stated ranges in some embodiments, to the tenth of the unit of the lower limit of the range, unless the context clearly dictates otherwise. For purposes of brevity, the values in each range have not been individually spelled out herein, but it will be understood that each of these values is provided herein and may be specifically claimed or disclaimed. It is also to be understood that unless otherwise indicated or otherwise evident from the context and/or the understanding of one of ordinary skill in the art, values expressed as ranges can assume any subrange within the given range, wherein the endpoints of the subrange are expressed to the same degree of accuracy as the tenth of the unit of the lower limit of the range.

In addition, it is to be understood that any particular embodiment of the present invention may be explicitly excluded from any one or more of the claims. Where ranges are given, any value within the range may explicitly be excluded from any one or more of the claims. Any embodiment, element, feature, application, or aspect of the compositions and/or methods of the invention, can be excluded from any one or more claims. For purposes of brevity, all of the embodiments in which one or more elements, features, purposes, or aspects is excluded are not set forth explicitly herein. 

1. A recombinant Cas9 protein comprising an amino acid sequence that is at least 80%, identical to the amino acid sequence of a Cas9 as provided by any one of SEQ ID NOs: 9-262, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation in an amino acid residue selected from the group consisting of amino acid residues 262, 267, 294, 405, 409, 480, 543, 694, 1219, 1224, 1256, and 1362 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein.
 2. The Cas9 protein of claim 1 comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of a Cas9 as provided by any one of SEQ ID NOs: 9-262.
 3. (canceled)
 4. The Cas9 protein of claim 1, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation selected from the group consisting of X262T, X267G, X294R, X405I, X409I, X480K, X543D, X694I, X1219V, X1224K, X1256K, and X1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.
 5. The Cas9 protein of claim 1, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation selected from the group consisting of A262T, S267G, K294R, F405I, S409I, E480K, E543D, M694I, E1219V, N1224K, Q1256K, and L1362P of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. 6-92. (canceled)
 93. A recombinant Cas9 protein comprising an amino acid sequence that is at least 80%, identical to the amino acid sequence of a Cas9 as provided by any one of SEQ ID NOs: 9-262, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation in an amino acid residue selected from the group consisting of amino acid residues 122, 137, 182, 262, 294, 409, 480, 543, 660, 694, 1219, and 1329 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. 94-123. (canceled)
 124. A recombinant Cas9 protein comprising an amino acid sequence that is at least 80% identical to the amino acid sequence of a Cas9 as provided by any one of SEQ ID NOs: 9-262, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation in an amino acid residue selected from the group consisting of amino acid residues 23, 108, 115, 141, 180, 230, 257, 262, 267, 284, 294, 324, 409, 455, 466, 474, 480, 543, 554, 654, 694, 711, 727, 763, 1063, 1100, 1219, 1244, 1256, 1289, and 1323 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein.
 125. (canceled)
 126. The Cas9 protein of claim 124, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation selected from the group consisting of X23N, X108G, X115H, X141Q, X180N, X230S, X257N, X262T, X267G, X284N, X294R, X324L, X409I, X455F, X466A, X474I, X480K, X543D, X554R, X654L, X694I, X711E, X727P, X763I, X1063V, X1100I, X1219V, X1244N, X1256K, X1289Q, and X1323S of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262, wherein X represents any amino acid.
 127. The Cas9 protein of any one of claim 124, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation selected from the group consisting of D23N, E108G, R115H, K141Q, D180N, P230S, D257N, A262T, S267G, D284N, K294R, R324L, S409I, L455F, T466A, T474I, E480K, E543D, K554R, R654L, M694I, A711E, L727P, M763I, I1063V, V1100I, E1219V, K1244N, Q1256K, K1289Q, and A1323S of the amino acid sequence provided in SEQ ID NO: 9, or a corresponding mutation in any of the amino acid sequences provided in SEQ ID NOs: 10-262. 128-275. (canceled)
 276. A recombinant Cas9 protein comprising an amino acid sequence that is at least 80% identical to the amino acid sequence of a Cas9 as provided by any one of SEQ ID NOs: 9-262, wherein the amino acid sequence of the Cas9 protein comprises at least one mutation in an amino acid residue selected from the group consisting of amino acid residues 108, 217, 262, 324, 409, 480, 543, 673, 694, 1219, 1264, and 1365 of the amino acid sequence provided in SEQ ID NO: 9, or in a corresponding amino acid residue in any of the amino acid sequences provided in SEQ ID NOs: 10-262, and wherein the amino acid sequence of the recombinant Cas9 protein is not identical to the amino acid sequence of a naturally occurring Cas9 protein. 277-345. (canceled)
 346. A fusion protein comprising the Cas9 protein of claim 1, wherein the Cas9 protein or fragment is fused to an effector domain, thus forming a fusion protein. 347-352. (canceled)
 353. The fusion protein of any one of claim 346, wherein the effector domain comprises an enzyme domain.
 354. The fusion protein of any one of claim 346, wherein the effector domain comprises a nuclease domain, a nickase domain, a recombinase domain, a deaminase domain, a methyltransferase domain, a methylase domain, an acetylase domain, an acetyltransferase domain, a transcriptional activator domain, or a transcriptional repressor domain. 355-370. (canceled)
 371. The fusion protein of claim 353, wherein the enzyme domain is a nuclease domain.
 372. (canceled)
 373. A dimer of the fusion protein of claim
 371. 374-381. (canceled)
 382. A fusion protein comprising the Cas9 protein of claim 124 fused to a second Cas9 protein. 383-390. (canceled)
 391. A complex comprising the Cas9 protein of claim 124, and a guide RNA bound to the Cas9 protein or the Cas9 fusion protein. 392-398. (canceled)
 399. A complex comprising the fusion protein of claim 382, a first guide RNA bound to the Cas9 protein of the fusion protein, and a second guide RNA bound to the second Cas9 protein of the fusion protein. 400-410. (canceled)
 411. A method comprising contacting a DNA molecule: (a) with the Cas9 protein of claim 93 and with a guide RNA, wherein the guide RNA is about 15-100 nucleotides long and comprises a sequence of at least 10 contiguous nucleotides that is complementary to a target sequence. 412-428. (canceled)
 429. A polynucleotide encoding the Cas9 protein of claim
 124. 430. A vector comprising a polynucleotide of claim
 429. 431. (canceled)
 432. A cell comprising the Cas9 protein of claim
 124. 433. A mutated Cas9 protein that recognizes a non-canonical PAM sequence selected from the group consisting of AAA, AAC, AAG, AAT, CAA, CAC, CAG, CAT, GAA, GAC, GAG, GAT, TAA, TAC, TAG, TAT, ACA, ACC, ACG, ACT, CCA, CCC, CCG, CCT, GCA, GCC, GCG, GCT, TCA, TCC, TCG, TCT, AGA, AGC, AGT, CGA, CGC, CGT, GGA, GGC, GGT, TGA, TGC, TGT, ATA, ATC, ATG, ATT, CTA, CTC, CTG, CTT, GTA, GTC, GTG, GTT, TTA, TTC, TTG, and TTT.
 434. (canceled) 